NAT8L cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NAT8L
Entrez Gene ID	339983
Full Name	N-acetyltransferase 8 like
Synonyms	CML3,NACED,NAT8-LIKE
General protein information	Preferred Names N-acetyltransferase 8 like Names N-acetylaspartate synthetase N-acetyltransferase 8-like (GCN5-related, putative) NAA synthetase Shati camello-like protein 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4p16.3
Summary	This gene encodes a single-pass membrane protein, which contains a conserved sequence of the GCN5 or NAT superfamily of N-acetyltransferases and is a member of the N-acyltransferase (NAT) superfamily. This protein is a neuron-specific protein and is the N-acetylaspartate (NAA) biosynthetic enzyme, catalyzing the NAA synthesis from L-aspartate and acetyl-CoA. NAA is a major storage and transport form of acetyl coenzyme A specific to the nervous system. The gene mutation results in primary NAA deficiency (hypoacetylaspartia). [provided by RefSeq, Dec 2010].
Disorder MIM:	610647
Disorder Html:	?N-acetylaspartate deficiency, 614063 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_178557.3	NP_848652.2	N-acetylaspartate synthetase
NM_178557.4	NP_848652.2	N-acetylaspartate synthetase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NAT8L	XP_853267.2
Homo sapiens (human)	NAT8L	NP_848652.2
Mus musculus (house mouse)	Nat8l	NP_001001985.3
Xenopus tropicalis (tropical clawed frog)	nat8l	NP_001096350.1
Rattus norvegicus (Norway rat)	Nat8l	NP_001178610.1
Gallus gallus (chicken)	NAT8L	XP_420835.4
Danio rerio (zebrafish)	nat8l	NP_001077308.1

Gene Ontology
Bibliography

Related articles in PubMed

Purification and characterization of aspartate N-acetyltransferase: A critical enzyme in brain metabolism.
Wang Q, Zhao M, Parungao GG, Viola RE
Protein expression and purification11911-8(2016 Mar)

Decreased DNA Methylation in the Shati/Nat8l Promoter in Both Patients with Schizophrenia and a Methamphetamine-Induced Murine Model of Schizophrenia-Like Phenotype.
Uno K, Kikuchi Y, Iwata M, Uehara T, Matsuoka T, Sumiyoshi T, Okamoto Y, Jinno H, Takada T, Furukawa-Hibi Y, Nabeshima T, Miyamoto Y, Nitta A
PloS one11(6)e0157959(2016)

Cancer-Specific Production of N-Acetylaspartate via NAT8L Overexpression in Non-Small Cell Lung Cancer and Its Potential as a Circulating Biomarker.
Lou TF, Sethuraman D, Dospoy P, Srivastva P, Kim HS, Kim J, Ma X, Chen PH, Huffman KE, Frink RE, Larsen JE, Lewis C, Um SW, Kim DH, Ahn JM, DeBerardinis RJ, White MA, Minna JD, Yoo H
Cancer prevention research (Philadelphia, Pa.)9(1)43-52(2016 Jan)

Deletion of SHATI/NAT8L increases dopamine D1 receptor on the cell surface in the nucleus accumbens, accelerating methamphetamine dependence.
Toriumi K, Kondo M, Nagai T, Hashimoto R, Ohi K, Song Z, Tanaka J, Mouri A, Koseki T, Yamamori H, Furukawa-Hibi Y, Mamiya T, Fukushima T, Takeda M, Nitta A, Yamada K, Nabeshima T
The international journal of neuropsychopharmacology17(3)443-53(2014 Mar)

NAT8L (N-acetyltransferase 8-like) accelerates lipid turnover and increases energy expenditure in brown adipocytes.
Pessentheiner AR, Pelzmann HJ, Walenta E, Schweiger M, Groschner LN, Graier WF, Kolb D, Uno K, Miyazaki T, Nitta A, Rieder D, Prokesch A, Bogner-Strauss JG
The Journal of biological chemistry288(50)36040-51(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Characterization and recombinant expression of the nat8l gene that codes for ANAT.
Title: Purification and characterization of aspartate N-acetyltransferase: A critical enzyme in brain metabolism.

Nat8l CpG island methylation ratios were lower in the patients with schizophrenia than in the healthy controls.
Title: Decreased DNA Methylation in the Shati/Nat8l Promoter in Both Patients with Schizophrenia and a Methamphetamine-Induced Murine Model of Schizophrenia-Like Phenotype.

NAA is produced specifically in NSCLC tumors through NAT8L overexpression, and its extracellular secretion can be detected in blood
Title: Cancer-Specific Production of N-Acetylaspartate via NAT8L Overexpression in Non-Small Cell Lung Cancer and Its Potential as a Circulating Biomarker.

This study demonistrated that the human NAT8L gene related to reward dependence, a personality trait, and grey matter volume in the caudate nucleus in healthy subjects, suggesting that NAT8L might also affect human personality
Title: Deletion of SHATI/NAT8L increases dopamine D1 receptor on the cell surface in the nucleus accumbens, accelerating methamphetamine dependence.

Nat8l impacts on the brown adipogenic phenotype
Title: NAT8L (N-acetyltransferase 8-like) accelerates lipid turnover and increases energy expenditure in brown adipocytes.

The following NAT8L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAT8L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22412	NM_178557.4 Latest version!	Homo sapiens N-acetyltransferase 8 like (NAT8L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu22412	NM_178557.3	Homo sapiens N-acetyltransferase 8 like (NAT8L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAT8L ( NM_178557.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_848652.2 Homo sapiens N-acetyltransferase 8 like (NAT8L), mRNA.

Price & Availability↑

CloneID	OHu22412
Clone ID Related Accession (Same CDS sequence)	NM_178557.3 , NM_178557.4
Accession Version	NM_178557.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	N-acetylaspartate synthetase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL132868.24, BC103748.1 and BC010045.2. This sequence is a reference standard in the RefSeqGene project. On or before Aug 31, 2013 this sequence version replaced XM_003403774.3, XM_003119921.1, NM_178557.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on a combination of same-species and homologous transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK094797.1, BC103748.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATTGTG GGCCTCCCGA CATGGTCTGC GAGACGAAGA TCGTGGCCGC CGAGGACCAT 
GAGGCGCTGC CGGGGGCCAA GAAGGACGCG CTGCTCGCCG CCGCCGGCGC CATGTGGCCC 
CCGCTGCCCG CCGCGCCCGG GCCGGCCGCC GCGCCCCCCG CGCCCCCACC TGCCCCGGTG 
GCTCAGCCTC ACGGCGGGGC GGGGGGCGCG GGGCCGCCGG GGGGGCGCGG CGTGTGCATC 
CGCGAGTTCC GTGCGGCCGA GCAGGAGGCG GCGCGCCGCA TCTTCTACGA CGGCATCATG 
GAGCGCATCC CTAACACGGC CTTCCGCGGC CTGCGGCAGC ACCCGCGCGC GCAGCTGCTC 
TACGCCCTGC TGGCGGCGCT GTGCTTCGCC GTGAGCCGCT CGCTGCTGCT GACGTGCCTG 
GTGCCGGCCG CGCTGCTGGG CCTGCGCTAC TACTACAGCC GCAAGGTGAT CCGCGCCTAC 
CTGGAGTGCG CGCTGCACAC GGACATGGCG GACATCGAGC AGTACTACAT GAAGCCGCCC 
GGCTCCTGCT TCTGGGTGGC CGTGCTGGAT GGCAACGTGG TGGGCATTGT GGCTGCACGG 
GCCCACGAGG AGGACAACAC GGTGGAGCTG CTGCGGATGT CTGTGGACTC ACGTTTCCGA 
GGCAAGGGCA TCGCCAAGGC GCTGGGCCGG AAGGTGCTGG AGTTCGCCGT GGTGCACAAC 
TACTCCGCGG TGGTGCTGGG CACGACGGCC GTCAAGGTGG CCGCCCACAA GCTCTACGAG 
TCGCTGGGCT TCAGACACAT GGGCGCCAGT GACCACTACG TGCTGCCGGG CATGACCCTC 
TCGCTGGCTG AGCGCCTCTT CTTCCAGGTC CGCTACCACC GCTACCGCCT GCAGCTGCGC 
GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848652.2
CDS	1..909
Translation	MHCGPPDMVCETKIVAAEDHEALPGAKKDALLAAAGAMWPPLPAAPGPAAAPPAPPPAPVAQPHGGAGGAGPPGGRGVCIREFRAAEQEAARRIFYDGIMERIPNTAFRGLRQHPRAQLLYALLAALCFAVSRSLLLTCLVPAALLGLRYYYSRKVIRAYLECALHTDMADIEQYYMKPPGSCFWVAVLDGNVVGIVAARAHEEDNTVELLRMSVDSRFRGKGIAKALGRKVLEFAVVHNYSAVVLGTTAVKVAAHKLYESLGFRHMGASDHYVLPGMTLSLAERLFFQVRYHRYRLQLREE

Target ORF information:

RefSeq Version	NM_178557.3
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acetyltransferase 8 like (NAT8L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178557.3

ORF Insert Sequence:

ATGCATTGTG GGCCTCCCGA CATGGTCTGC GAGACGAAGA TCGTGGCCGC CGAGGACCAT 
GAGGCGCTGC CGGGGGCCAA GAAGGACGCG CTGCTCGCCG CCGCCGGCGC CATGTGGCCC 
CCGCTGCCCG CCGCGCCCGG GCCGGCCGCC GCGCCCCCCG CGCCCCCACC TGCCCCGGTG 
GCTCAGCCTC ACGGCGGGGC GGGGGGCGCG GGGCCGCCGG GGGGGCGCGG CGTGTGCATC 
CGCGAGTTCC GTGCGGCCGA GCAGGAGGCG GCGCGCCGCA TCTTCTACGA CGGCATCATG 
GAGCGCATCC CTAACACGGC CTTCCGCGGC CTGCGGCAGC ACCCGCGCGC GCAGCTGCTC 
TACGCCCTGC TGGCGGCGCT GTGCTTCGCC GTGAGCCGCT CGCTGCTGCT GACGTGCCTG 
GTGCCGGCCG CGCTGCTGGG CCTGCGCTAC TACTACAGCC GCAAGGTGAT CCGCGCCTAC 
CTGGAGTGCG CGCTGCACAC GGACATGGCG GACATCGAGC AGTACTACAT GAAGCCGCCC 
GGCTCCTGCT TCTGGGTGGC CGTGCTGGAT GGCAACGTGG TGGGCATTGT GGCTGCACGG 
GCCCACGAGG AGGACAACAC GGTGGAGCTG CTGCGGATGT CTGTGGACTC ACGTTTCCGA 
GGCAAGGGCA TCGCCAAGGC GCTGGGCCGG AAGGTGCTGG AGTTCGCCGT GGTGCACAAC 
TACTCCGCGG TGGTGCTGGG CACGACGGCC GTCAAGGTGG CCGCCCACAA GCTCTACGAG 
TCGCTGGGCT TCAGACACAT GGGCGCCAGT GACCACTACG TGCTGCCGGG CATGACCCTC 
TCGCTGGCTG AGCGCCTCTT CTTCCAGGTC CGCTACCACC GCTACCGCCT GCAGCTGCGC 
GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAT8L ( NM_178557.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_848652.2 Homo sapiens N-acetyltransferase 8 like (NAT8L), mRNA.

Price & Availability↑

CloneID	OHu22412
Clone ID Related Accession (Same CDS sequence)	NM_178557.3 , NM_178557.4
Accession Version	NM_178557.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	N-acetylaspartate synthetase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL132868.24, BC103748.1 and BC010045.2. This sequence is a reference standard in the RefSeqGene project. On Aug 27, 2018 this sequence version replaced NM_178557.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK094797.1, BC103748.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCATTGTG GGCCTCCCGA CATGGTCTGC GAGACGAAGA TCGTGGCCGC CGAGGACCAT 
GAGGCGCTGC CGGGGGCCAA GAAGGACGCG CTGCTCGCCG CCGCCGGCGC CATGTGGCCC 
CCGCTGCCCG CCGCGCCCGG GCCGGCCGCC GCGCCCCCCG CGCCCCCACC TGCCCCGGTG 
GCTCAGCCTC ACGGCGGGGC GGGGGGCGCG GGGCCGCCGG GGGGGCGCGG CGTGTGCATC 
CGCGAGTTCC GTGCGGCCGA GCAGGAGGCG GCGCGCCGCA TCTTCTACGA CGGCATCATG 
GAGCGCATCC CTAACACGGC CTTCCGCGGC CTGCGGCAGC ACCCGCGCGC GCAGCTGCTC 
TACGCCCTGC TGGCGGCGCT GTGCTTCGCC GTGAGCCGCT CGCTGCTGCT GACGTGCCTG 
GTGCCGGCCG CGCTGCTGGG CCTGCGCTAC TACTACAGCC GCAAGGTGAT CCGCGCCTAC 
CTGGAGTGCG CGCTGCACAC GGACATGGCG GACATCGAGC AGTACTACAT GAAGCCGCCC 
GGCTCCTGCT TCTGGGTGGC CGTGCTGGAT GGCAACGTGG TGGGCATTGT GGCTGCACGG 
GCCCACGAGG AGGACAACAC GGTGGAGCTG CTGCGGATGT CTGTGGACTC ACGTTTCCGA 
GGCAAGGGCA TCGCCAAGGC GCTGGGCCGG AAGGTGCTGG AGTTCGCCGT GGTGCACAAC 
TACTCCGCGG TGGTGCTGGG CACGACGGCC GTCAAGGTGG CCGCCCACAA GCTCTACGAG 
TCGCTGGGCT TCAGACACAT GGGCGCCAGT GACCACTACG TGCTGCCGGG CATGACCCTC 
TCGCTGGCTG AGCGCCTCTT CTTCCAGGTC CGCTACCACC GCTACCGCCT GCAGCTGCGC 
GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848652.2
CDS	186..1094
Translation	MHCGPPDMVCETKIVAAEDHEALPGAKKDALLAAAGAMWPPLPAAPGPAAAPPAPPPAPVAQPHGGAGGAGPPGGRGVCIREFRAAEQEAARRIFYDGIMERIPNTAFRGLRQHPRAQLLYALLAALCFAVSRSLLLTCLVPAALLGLRYYYSRKVIRAYLECALHTDMADIEQYYMKPPGSCFWVAVLDGNVVGIVAARAHEEDNTVELLRMSVDSRFRGKGIAKALGRKVLEFAVVHNYSAVVLGTTAVKVAAHKLYESLGFRHMGASDHYVLPGMTLSLAERLFFQVRYHRYRLQLREE

Target ORF information:

RefSeq Version	NM_178557.4
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acetyltransferase 8 like (NAT8L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178557.4

ORF Insert Sequence:

ATGCATTGTG GGCCTCCCGA CATGGTCTGC GAGACGAAGA TCGTGGCCGC CGAGGACCAT 
GAGGCGCTGC CGGGGGCCAA GAAGGACGCG CTGCTCGCCG CCGCCGGCGC CATGTGGCCC 
CCGCTGCCCG CCGCGCCCGG GCCGGCCGCC GCGCCCCCCG CGCCCCCACC TGCCCCGGTG 
GCTCAGCCTC ACGGCGGGGC GGGGGGCGCG GGGCCGCCGG GGGGGCGCGG CGTGTGCATC 
CGCGAGTTCC GTGCGGCCGA GCAGGAGGCG GCGCGCCGCA TCTTCTACGA CGGCATCATG 
GAGCGCATCC CTAACACGGC CTTCCGCGGC CTGCGGCAGC ACCCGCGCGC GCAGCTGCTC 
TACGCCCTGC TGGCGGCGCT GTGCTTCGCC GTGAGCCGCT CGCTGCTGCT GACGTGCCTG 
GTGCCGGCCG CGCTGCTGGG CCTGCGCTAC TACTACAGCC GCAAGGTGAT CCGCGCCTAC 
CTGGAGTGCG CGCTGCACAC GGACATGGCG GACATCGAGC AGTACTACAT GAAGCCGCCC 
GGCTCCTGCT TCTGGGTGGC CGTGCTGGAT GGCAACGTGG TGGGCATTGT GGCTGCACGG 
GCCCACGAGG AGGACAACAC GGTGGAGCTG CTGCGGATGT CTGTGGACTC ACGTTTCCGA 
GGCAAGGGCA TCGCCAAGGC GCTGGGCCGG AAGGTGCTGG AGTTCGCCGT GGTGCACAAC 
TACTCCGCGG TGGTGCTGGG CACGACGGCC GTCAAGGTGG CCGCCCACAA GCTCTACGAG 
TCGCTGGGCT TCAGACACAT GGGCGCCAGT GACCACTACG TGCTGCCGGG CATGACCCTC 
TCGCTGGCTG AGCGCCTCTT CTTCCAGGTC CGCTACCACC GCTACCGCCT GCAGCTGCGC 
GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Purification and characterization of aspartate N-acetyltransferase: A critical enzyme in brain metabolism.
Protein expression and purification11911-8(2016 Mar)
Wang Q,Zhao M,Parungao GG,Viola RE

Decreased DNA Methylation in the Shati/Nat8l Promoter in Both Patients with Schizophrenia and a Methamphetamine-Induced Murine Model of Schizophrenia-Like Phenotype.
PloS one11(6)e0157959(2016)
Uno K,Kikuchi Y,Iwata M,Uehara T,Matsuoka T,Sumiyoshi T,Okamoto Y,Jinno H,Takada T,Furukawa-Hibi Y,Nabeshima T,Miyamoto Y,Nitta A

Cancer-Specific Production of N-Acetylaspartate via NAT8L Overexpression in Non-Small Cell Lung Cancer and Its Potential as a Circulating Biomarker.
Cancer prevention research (Philadelphia, Pa.)9(1)43-52(2016 Jan)
Lou TF,Sethuraman D,Dospoy P,Srivastva P,Kim HS,Kim J,Ma X,Chen PH,Huffman KE,Frink RE,Larsen JE,Lewis C,Um SW,Kim DH,Ahn JM,DeBerardinis RJ,White MA,Minna JD,Yoo H

Deletion of SHATI/NAT8L increases dopamine D1 receptor on the cell surface in the nucleus accumbens, accelerating methamphetamine dependence.
The international journal of neuropsychopharmacology17(3)443-53(2014 Mar)
Toriumi K,Kondo M,Nagai T,Hashimoto R,Ohi K,Song Z,Tanaka J,Mouri A,Koseki T,Yamamori H,Furukawa-Hibi Y,Mamiya T,Fukushima T,Takeda M,Nitta A,Yamada K,Nabeshima T

NAT8L (N-acetyltransferase 8-like) accelerates lipid turnover and increases energy expenditure in brown adipocytes.
The Journal of biological chemistry288(50)36040-51(2013 Dec)
Pessentheiner AR,Pelzmann HJ,Walenta E,Schweiger M,Groschner LN,Graier WF,Kolb D,Uno K,Miyazaki T,Nitta A,Rieder D,Prokesch A,Bogner-Strauss JG

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NAT8L cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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