B4GALNT4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	B4GALNT4
Entrez Gene ID	338707
Full Name	beta-1,4-N-acetyl-galactosaminyltransferase 4
General protein information	Preferred Names beta-1,4-N-acetyl-galactosaminyltransferase 4 Names N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1 N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N- acetylgalactosaminyltransferase NGalNAc-T1 beta-1,4-N-acetyl-galactosaminyl transferase 4 beta-1,4-N-acetylgalactosaminyltransferase IV beta1,4-N-acetylgalactosaminyltransferases IV beta4GalNAc-T4 beta4GalNAcT4 betaGT4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.5

mRNA and Protein(s)

mRNA	Protein	Name
XM_017017654.1	XP_016873143.1	N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1 isoform X1
NM_178537.4	NP_848632.2	N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1
NM_178537.5	NP_848632.2	N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	B4GALNT4	XP_003312892.1
Rattus norvegicus (Norway rat)	B4galnt4	NP_001101032.1
Gallus gallus (chicken)	B4GALNT4	XP_001233941.3
Danio rerio (zebrafish)	LOC559305	XP_687729.4
Danio rerio (zebrafish)	LOC565184	XP_001920875.4
Mus musculus (house mouse)	B4galnt4	NP_808565.2
Xenopus tropicalis (tropical clawed frog)	b4galnt4	NP_001090669.1
Homo sapiens (human)	B4GALNT4	NP_848632.2

Gene Ontology
Bibliography

Related articles in PubMed

Enhanced expression of the ?4-N-acetylgalactosaminyltransferase 4?gene impairs tumor growth of human breast cancer cells.
Hirano K, Matsuda A, Kuji R, Nakandakari S, Shirai T, Furukawa K
Biochemical and biophysical research communications461(1)80-5(2015 May)

Molecular basis for protein-specific transfer of N-acetylgalactosamine to N-linked glycans by the glycosyltransferases ?1,4-N-acetylgalactosaminyl transferase 3 (?4GalNAc-T3) and ?4GalNAc-T4.
Fiete D, Beranek M, Baenziger JU
The Journal of biological chemistry287(34)29194-203(2012 Aug)

Peptide-specific transfer of N-acetylgalactosamine to O-linked glycans by the glycosyltransferases ?1,4-N-acetylgalactosaminyl transferase 3 (?4GalNAc-T3) and ?4GalNAc-T4.
Fiete D, Beranek M, Baenziger JU
The Journal of biological chemistry287(34)29204-12(2012 Aug)

A necessary and sufficient determinant for protein-selective glycosylation in vivo.
Miller E, Fiete D, Blake NM, Beranek M, Oates EL, Mi Y, Roseman DS, Baenziger JU
The Journal of biological chemistry283(4)1985-91(2008 Jan)

Molecular cloning and characterization of beta1,4-N-acetylgalactosaminyltransferases IV synthesizing N,N'-diacetyllactosediamine.
Gotoh M, Sato T, Kiyohara K, Kameyama A, Kikuchi N, Kwon YD, Ishizuka Y, Iwai T, Nakanishi H, Narimatsu H
FEBS letters562(1-3)134-40(2004 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate that the expression of the LacdiNAc group is quite important for the suppression of malignancies of the MDA-MB-231 cells.
Title: Enhanced expression of the ?4-N-acetylgalactosaminyltransferase 4?gene impairs tumor growth of human breast cancer cells.

Molecular basis for protein-specific transfer of N-acetylgalactosamine to N-linked glycans by the glycosyltransferases beta1,4-N-acetylgalactosaminyl transferase 3 (beta4GalNAc-T3) and beta4GalNAc-T4.
Title: Molecular basis for protein-specific transfer of N-acetylgalactosamine to N-linked glycans by the glycosyltransferases ?1,4-N-acetylgalactosaminyl transferase 3 (?4GalNAc-T3) and ?4GalNAc-T4.

Peptide-specific transfer of N-acetylgalactosamine to O-linked glycans by the glycosyltransferases beta1,4-N-acetylgalactosaminyl transferase 3 (beta4GalNAc-T3) and beta4GalNAc-T4.
Title: Peptide-specific transfer of N-acetylgalactosamine to O-linked glycans by the glycosyltransferases ?1,4-N-acetylgalactosaminyl transferase 3 (?4GalNAc-T3) and ?4GalNAc-T4.

betaGT3 and betaGT4, that are able to transfer GalNAc to GlcNAc in beta1,4-linkage display the necessary glycoprotein specificity in vivo.
Title: A necessary and sufficient determinant for protein-selective glycosylation in vivo.

molecular cloning and characterization; specificity toward oligosaccharide acceptor substrates was quite similar to beta4GalNAc-T3 in vitro but the tissue distributions of the two enzymes were quite different
Title: Molecular cloning and characterization of beta1,4-N-acetylgalactosaminyltransferases IV synthesizing N,N'-diacetyllactosediamine.

The following B4GALNT4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B4GALNT4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31346	NM_178537.5 Latest version!	Homo sapiens beta-1,4-N-acetyl-galactosaminyltransferase 4 (B4GALNT4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$734.30 $1049.00
OHu92187	XM_017017654.1 Latest version!	Homo sapiens beta-1,4-N-acetyl-galactosaminyltransferase 4 (B4GALNT4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu31346	NM_178537.4	Homo sapiens beta-1,4-N-acetyl-galactosaminyltransferase 4 (B4GALNT4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$734.30 $1049.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B4GALNT4 ( NM_178537.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_848632.2 Homo sapiens beta-1,4-N-acetyl-galactosaminyltransferase 4 (B4GALNT4), mRNA.

Price & Availability↑

CloneID	OHu31346
Clone ID Related Accession (Same CDS sequence)	NM_178537.4 , NM_178537.5
Accession Version	NM_178537.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BF058197.1, AB089939.1 and CN348077.1. On Apr 25, 2009 this sequence version replaced NM_178537.3. ##Evidence-Data-START## Transcript exon combination :: AB089939.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGCGGC TCCCGGTGAA GAAGATCCGT AAGCAGATGA AGCTGCTGCT GCTGCTGCTG 
CTGCTGAGCT GCGCCGCGTG GCTCACCTAC GTGCACCTGG GCCTGGTGCG CCAGGGACGC 
GCGCTGCGCC AGCGCCTGGG CTACGGGCGA GATGGTGAGA AGCTGACCAG TGAGACCGAC 
GGCCGGGGGG TCCACGCTGC GCCATCCACA CAGAGGGCTG AGGACTCCAG TGAGAGCCGT 
GAAGAGGAGC AAGCGCCCGA AGGTCGGGAC CTAGACATGC TGTTTCCTGG GGGGGCTGGG 
AGGCTGCCAC TGAACTTCAC CCATCAGACA CCCCCATGGC GGGAGGAGTA CAAGGGGCAG 
GTGAACCTGC ACGTGTTTGA GGACTGGTGT GGGGGCGCCG TGGGCCACCT GAGGAGGAAC 
CTGCACTTCC CGCTGTTCCC TCATACGCGC ACCACCGTGA AGAAGTTGGC CGTGTCCCCC 
AAGTGGAAGA ACTATGGACT CCGTATTTTT GGTTTCATCC ACCCGGCGAG GGACGGAGAC 
GTCCAGTTTT CTGTGGCCTC AGACGACAAC TCGGAGTTCT GGCTGAGTCT GGACGAGAGC 
CCTGCTGCTG CCCAGCTTGT GGCCTTTGTG GGCAAGACTG GCTCCGAGTG GACAGCGCCT 
GGAGAATTCA CCAAGTTCAG CTCCCAGGTG TCCAAGCCCA GGCGGCTCAT GGCCTCCCGG 
AGGTACTACT TTGAGTTGCT GCACAAGCAG GACGACCGCG GCTCGGACCA CGTGGAAGTG 
GGCTGGCGAG CTTTCCTGCC CGGCCTGAAG TTCGAGGTCA TCAGCTCTGC TCACATCTCC 
CTGTACACAG ATGAGTCAGC CTTGAAGATG GACCACGTGG CGCACGTCCC CCAGTCTCCA 
GCCAGCCACG TGGGGGGGCG TCCGCCGCAG GAGGAGACCA GCGCAGACAT GCTGCGGCCA 
GATCCCAGGG ATACCTTTTT CCTCACTCCA CGCATGGAAT CTTCGAGCCT GGAGAACGTG 
CTGGAGCCCT GCGCCTACGC CCCCACCTAC GTGGTCAAGG ACTTCCCGAT CGCCAGATAC 
CAGGGCCTGC AATTTGTGTA CCTGTCCTTC GTTTATCCCA ACGACTACAC TCGCCTCACC 
CACATGGAGA CGGACAACAA GTGCTTCTAC CGCGAGTCTC CGCTGTATCT GGAGAGGTTT 
GGGTTCTATA AATACATGAA GATGGACAAG GAGGAGGGGG ATGAGGATGA AGAAGACGAG 
GTGCAGCGCC GAGCCTTCCT CTTCCTCAAC CCGGACGACT TCCTGGACGA CGAGGACGAG 
GGGGAGCTGC TCGACAGCCT GGAGCCCACC GAGGCGGCCC CGCCCAGGAG CGGCCCCCAG 
TCCCCCGCCC CAGCAGCCCC CGCCCAGCCC GGAGCCACCC TCGCCCCGCC GACCCCTCCC 
CGCCCCCGGG ACGGGGGGAC CCCCAGGCAC TCCCGGGCCC TGAGCTGGGC CGCCAGGGCC 
GCCCGCCCTT TGCCGCTCTT CTTGGGCCGA GCTCCGCCCC CGCGCCCTGC AGTGGAGCAG 
CCGCCCCCAA AGGTGTACGT GACCAGGGTG CGGCCGGGAC AGCGGGCATC CCCCCGGGCC 
CCAGCGCCGC GTGCGCCCTG GCCGCCCTTC CCTGGCGTCT TCCTGCACCC CAGGCCTCTG 
CCCAGAGTGC AGCTGCGGGC GCCCCCACGC CCACCCCGGC CCCACGGCCG CAGGACCGGC 
GGCCCCCAGG CCACACAGCC GAGGCCCCCA GCCCGGGCGC AGGCCACCCA AGGGGGCCGG 
GAGGGCCAGG CGCGCACGCT GGGACCTGCG GCGCCCACAG TGGACTCAAA CTTGTCCTCC 
GAAGCGCGGC CCGTGACCTC CTTCCTGAGC TTGTCCCAGG TGTCCGGGCC GCAGCTGCCC 
GGGGAGGGCG AAGAGGAGGA GGAAGGGGAG GACGATGGGG CCCCGGGCGA CGAGGCCGCG 
TCGGAGGACA GCGAGGAGGC CGCGGGCCCG GCGCTCGGAC GCTGGCGTGA GGACGCCATC 
GACTGGCAGC GCACGTTCAG CGTGGGCGCC GTGGACTTCG AGCTGCTGCG CTCGGACTGG 
AACGACCTGC GATGCAACGT TTCGGGGAAC CTGCAGCTGC CGGAGGCGGA GGCCGTGGAC 
GTGACCGCTC AGTACATGGA GCGGCTGAAC GCGCGCCACG GCGGGCGCTT CGCGCTTCTG 
CGCATCGTGA ACGTGGAGAA GCGCCGGGAC TCGGCGCGAG GGAGTCGCTT CCTGCTGGAG 
CTGGAGCTGC AGGAGCGCGG GGGCGGCCGC CTGCGACTGT CCGAGTACGT CTTCCTGCGG 
CTGCCGGGAG CCCGCGTAGG GGATGCAGAC GGAGAAAGTC CCGAACCCGC TCCCGCCGCC 
TCCGTGCGCC CCGACGGCCG CCCCGAGCTC TGCCGGCCAC TGCGCCTGGC CTGGCGCCAG 
GACGTGATGG TTCACTTCAT CGTGCCAGTG AAAAACCAGG CACGGTGGGT GGCACAGTTC 
CTGGCGGACA TGGCTGCGCT GCACGCGCGC ACCGGGGACT CGCGTTTCAG CGTCGTCCTG 
GTGGATTTCG AGAGCGAGGA TATGGACGTG GAGCGGGCCC TGCGCGCCGC GCGCCTGCCC 
CGGTACCAGT ACCTGAGACG AACCGGGAAC TTCGAGCGCT CCGCCGGGCT GCAGGCGGGA 
GTGGACGCGG TAGAGGACGC CAGCAGCATC GTGTTCCTCT GCGACCTGCA CATCCACTTC 
CCACCCAACA TCCTGGACGG CATCCGCAAG CACTGCGTGG AGGGCAGGCT GGCCTTCGCG 
CCCGTGGTCA TGCGCCTGAG CTGCGGGAGC TCGCCCCGGG ACCCCCACGG TTACTGGGAG 
GTGAACGGCT TTGGCCTTTT TGGGATCTAC AAGTCGGACT TTGACCGGGT TGGAGGAATG 
AACACGGAGG AGTTCCGAGA CCAGTGGGGG GGTGAAGACT GGGAGCTCCT GGACAGGGTC 
CTGCAGGCAG GGCTGGAGGT GGAGCGGCTC CGACTGCGGA ATTTCTATCA CCACTACCAC 
TCCAAGAGGG GCATGTGGAG CGTCCGCAGC AGGAAGGGCT CTCGCACGGG GGCGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848632.2
CDS	10..3129
Translation	MPRLPVKKIRKQMKLLLLLLLLSCAAWLTYVHLGLVRQGRALRQRLGYGRDGEKLTSETDGRGVHAAPSTQRAEDSSESREEEQAPEGRDLDMLFPGGAGRLPLNFTHQTPPWREEYKGQVNLHVFEDWCGGAVGHLRRNLHFPLFPHTRTTVKKLAVSPKWKNYGLRIFGFIHPARDGDVQFSVASDDNSEFWLSLDESPAAAQLVAFVGKTGSEWTAPGEFTKFSSQVSKPRRLMASRRYYFELLHKQDDRGSDHVEVGWRAFLPGLKFEVISSAHISLYTDESALKMDHVAHVPQSPASHVGGRPPQEETSADMLRPDPRDTFFLTPRMESSSLENVLEPCAYAPTYVVKDFPIARYQGLQFVYLSFVYPNDYTRLTHMETDNKCFYRESPLYLERFGFYKYMKMDKEEGDEDEEDEVQRRAFLFLNPDDFLDDEDEGELLDSLEPTEAAPPRSGPQSPAPAAPAQPGATLAPPTPPRPRDGGTPRHSRALSWAARAARPLPLFLGRAPPPRPAVEQPPPKVYVTRVRPGQRASPRAPAPRAPWPPFPGVFLHPRPLPRVQLRAPPRPPRPHGRRTGGPQATQPRPPARAQATQGGREGQARTLGPAAPTVDSNLSSEARPVTSFLSLSQVSGPQLPGEGEEEEEGEDDGAPGDEAASEDSEEAAGPALGRWREDAIDWQRTFSVGAVDFELLRSDWNDLRCNVSGNLQLPEAEAVDVTAQYMERLNARHGGRFALLRIVNVEKRRDSARGSRFLLELELQERGGGRLRLSEYVFLRLPGARVGDADGESPEPAPAASVRPDGRPELCRPLRLAWRQDVMVHFIVPVKNQARWVAQFLADMAALHARTGDSRFSVVLVDFESEDMDVERALRAARLPRYQYLRRTGNFERSAGLQAGVDAVEDASSIVFLCDLHIHFPPNILDGIRKHCVEGRLAFAPVVMRLSCGSSPRDPHGYWEVNGFGLFGIYKSDFDRVGGMNTEEFRDQWGGEDWELLDRVLQAGLEVERLRLRNFYHHYHSKRGMWSVRSRKGSRTGAS

Target ORF information:

RefSeq Version	NM_178537.4
Organism	Homo sapiens(human)
Definition	Homo sapiens beta-1,4-N-acetyl-galactosaminyltransferase 4 (B4GALNT4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178537.4

ORF Insert Sequence:

ATGCCGCGGC TCCCGGTGAA GAAGATCCGT AAGCAGATGA AGCTGCTGCT GCTGCTGCTG 
CTGCTGAGCT GCGCCGCGTG GCTCACCTAC GTGCACCTGG GCCTGGTGCG CCAGGGACGC 
GCGCTGCGCC AGCGCCTGGG CTACGGGCGA GATGGTGAGA AGCTGACCAG TGAGACCGAC 
GGCCGGGGGG TCCACGCTGC GCCATCCACA CAGAGGGCTG AGGACTCCAG TGAGAGCCGT 
GAAGAGGAGC AAGCGCCCGA AGGTCGGGAC CTAGACATGC TGTTTCCTGG GGGGGCTGGG 
AGGCTGCCAC TGAACTTCAC CCATCAGACA CCCCCATGGC GGGAGGAGTA CAAGGGGCAG 
GTGAACCTGC ACGTGTTTGA GGACTGGTGT GGGGGCGCCG TGGGCCACCT GAGGAGGAAC 
CTGCACTTCC CGCTGTTCCC TCATACGCGC ACCACCGTGA AGAAGTTGGC CGTGTCCCCC 
AAGTGGAAGA ACTATGGACT CCGTATTTTT GGTTTCATCC ACCCGGCGAG GGACGGAGAC 
GTCCAGTTTT CTGTGGCCTC AGACGACAAC TCGGAGTTCT GGCTGAGTCT GGACGAGAGC 
CCTGCTGCTG CCCAGCTTGT GGCCTTTGTG GGCAAGACTG GCTCCGAGTG GACAGCGCCT 
GGAGAATTCA CCAAGTTCAG CTCCCAGGTG TCCAAGCCCA GGCGGCTCAT GGCCTCCCGG 
AGGTACTACT TTGAGTTGCT GCACAAGCAG GACGACCGCG GCTCGGACCA CGTGGAAGTG 
GGCTGGCGAG CTTTCCTGCC CGGCCTGAAG TTCGAGGTCA TCAGCTCTGC TCACATCTCC 
CTGTACACAG ATGAGTCAGC CTTGAAGATG GACCACGTGG CGCACGTCCC CCAGTCTCCA 
GCCAGCCACG TGGGGGGGCG TCCGCCGCAG GAGGAGACCA GCGCAGACAT GCTGCGGCCA 
GATCCCAGGG ATACCTTTTT CCTCACTCCA CGCATGGAAT CTTCGAGCCT GGAGAACGTG 
CTGGAGCCCT GCGCCTACGC CCCCACCTAC GTGGTCAAGG ACTTCCCGAT CGCCAGATAC 
CAGGGCCTGC AATTTGTGTA CCTGTCCTTC GTTTATCCCA ACGACTACAC TCGCCTCACC 
CACATGGAGA CGGACAACAA GTGCTTCTAC CGCGAGTCTC CGCTGTATCT GGAGAGGTTT 
GGGTTCTATA AATACATGAA GATGGACAAG GAGGAGGGGG ATGAGGATGA AGAAGACGAG 
GTGCAGCGCC GAGCCTTCCT CTTCCTCAAC CCGGACGACT TCCTGGACGA CGAGGACGAG 
GGGGAGCTGC TCGACAGCCT GGAGCCCACC GAGGCGGCCC CGCCCAGGAG CGGCCCCCAG 
TCCCCCGCCC CAGCAGCCCC CGCCCAGCCC GGAGCCACCC TCGCCCCGCC GACCCCTCCC 
CGCCCCCGGG ACGGGGGGAC CCCCAGGCAC TCCCGGGCCC TGAGCTGGGC CGCCAGGGCC 
GCCCGCCCTT TGCCGCTCTT CTTGGGCCGA GCTCCGCCCC CGCGCCCTGC AGTGGAGCAG 
CCGCCCCCAA AGGTGTACGT GACCAGGGTG CGGCCGGGAC AGCGGGCATC CCCCCGGGCC 
CCAGCGCCGC GTGCGCCCTG GCCGCCCTTC CCTGGCGTCT TCCTGCACCC CAGGCCTCTG 
CCCAGAGTGC AGCTGCGGGC GCCCCCACGC CCACCCCGGC CCCACGGCCG CAGGACCGGC 
GGCCCCCAGG CCACACAGCC GAGGCCCCCA GCCCGGGCGC AGGCCACCCA AGGGGGCCGG 
GAGGGCCAGG CGCGCACGCT GGGACCTGCG GCGCCCACAG TGGACTCAAA CTTGTCCTCC 
GAAGCGCGGC CCGTGACCTC CTTCCTGAGC TTGTCCCAGG TGTCCGGGCC GCAGCTGCCC 
GGGGAGGGCG AAGAGGAGGA GGAAGGGGAG GACGATGGGG CCCCGGGCGA CGAGGCCGCG 
TCGGAGGACA GCGAGGAGGC CGCGGGCCCG GCGCTCGGAC GCTGGCGTGA GGACGCCATC 
GACTGGCAGC GCACGTTCAG CGTGGGCGCC GTGGACTTCG AGCTGCTGCG CTCGGACTGG 
AACGACCTGC GATGCAACGT TTCGGGGAAC CTGCAGCTGC CGGAGGCGGA GGCCGTGGAC 
GTGACCGCTC AGTACATGGA GCGGCTGAAC GCGCGCCACG GCGGGCGCTT CGCGCTTCTG 
CGCATCGTGA ACGTGGAGAA GCGCCGGGAC TCGGCGCGAG GGAGTCGCTT CCTGCTGGAG 
CTGGAGCTGC AGGAGCGCGG GGGCGGCCGC CTGCGACTGT CCGAGTACGT CTTCCTGCGG 
CTGCCGGGAG CCCGCGTAGG GGATGCAGAC GGAGAAAGTC CCGAACCCGC TCCCGCCGCC 
TCCGTGCGCC CCGACGGCCG CCCCGAGCTC TGCCGGCCAC TGCGCCTGGC CTGGCGCCAG 
GACGTGATGG TTCACTTCAT CGTGCCAGTG AAAAACCAGG CACGGTGGGT GGCACAGTTC 
CTGGCGGACA TGGCTGCGCT GCACGCGCGC ACCGGGGACT CGCGTTTCAG CGTCGTCCTG 
GTGGATTTCG AGAGCGAGGA TATGGACGTG GAGCGGGCCC TGCGCGCCGC GCGCCTGCCC 
CGGTACCAGT ACCTGAGACG AACCGGGAAC TTCGAGCGCT CCGCCGGGCT GCAGGCGGGA 
GTGGACGCGG TAGAGGACGC CAGCAGCATC GTGTTCCTCT GCGACCTGCA CATCCACTTC 
CCACCCAACA TCCTGGACGG CATCCGCAAG CACTGCGTGG AGGGCAGGCT GGCCTTCGCG 
CCCGTGGTCA TGCGCCTGAG CTGCGGGAGC TCGCCCCGGG ACCCCCACGG TTACTGGGAG 
GTGAACGGCT TTGGCCTTTT TGGGATCTAC AAGTCGGACT TTGACCGGGT TGGAGGAATG 
AACACGGAGG AGTTCCGAGA CCAGTGGGGG GGTGAAGACT GGGAGCTCCT GGACAGGGTC 
CTGCAGGCAG GGCTGGAGGT GGAGCGGCTC CGACTGCGGA ATTTCTATCA CCACTACCAC 
TCCAAGAGGG GCATGTGGAG CGTCCGCAGC AGGAAGGGCT CTCGCACGGG GGCGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B4GALNT4 ( NM_178537.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_848632.2 Homo sapiens beta-1,4-N-acetyl-galactosaminyltransferase 4 (B4GALNT4), mRNA.

Price & Availability↑

CloneID	OHu31346
Clone ID Related Accession (Same CDS sequence)	NM_178537.4 , NM_178537.5
Accession Version	NM_178537.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC138230.5, BF058197.1, AB089939.1 and CN348077.1. On Nov 22, 2018 this sequence version replaced NM_178537.4. ##Evidence-Data-START## Transcript exon combination :: AB089939.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000329962.11/ ENSP00000328277.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGCGGC TCCCGGTGAA GAAGATCCGT AAGCAGATGA AGCTGCTGCT GCTGCTGCTG 
CTGCTGAGCT GCGCCGCGTG GCTCACCTAC GTGCACCTGG GCCTGGTGCG CCAGGGACGC 
GCGCTGCGCC AGCGCCTGGG CTACGGGCGA GATGGTGAGA AGCTGACCAG TGAGACCGAC 
GGCCGGGGGG TCCACGCTGC GCCATCCACA CAGAGGGCTG AGGACTCCAG TGAGAGCCGT 
GAAGAGGAGC AAGCGCCCGA AGGTCGGGAC CTAGACATGC TGTTTCCTGG GGGGGCTGGG 
AGGCTGCCAC TGAACTTCAC CCATCAGACA CCCCCATGGC GGGAGGAGTA CAAGGGGCAG 
GTGAACCTGC ACGTGTTTGA GGACTGGTGT GGGGGCGCCG TGGGCCACCT GAGGAGGAAC 
CTGCACTTCC CGCTGTTCCC TCATACGCGC ACCACCGTGA AGAAGTTGGC CGTGTCCCCC 
AAGTGGAAGA ACTATGGACT CCGTATTTTT GGTTTCATCC ACCCGGCGAG GGACGGAGAC 
GTCCAGTTTT CTGTGGCCTC AGACGACAAC TCGGAGTTCT GGCTGAGTCT GGACGAGAGC 
CCTGCTGCTG CCCAGCTTGT GGCCTTTGTG GGCAAGACTG GCTCCGAGTG GACAGCGCCT 
GGAGAATTCA CCAAGTTCAG CTCCCAGGTG TCCAAGCCCA GGCGGCTCAT GGCCTCCCGG 
AGGTACTACT TTGAGTTGCT GCACAAGCAG GACGACCGCG GCTCGGACCA CGTGGAAGTG 
GGCTGGCGAG CTTTCCTGCC CGGCCTGAAG TTCGAGGTCA TCAGCTCTGC TCACATCTCC 
CTGTACACAG ATGAGTCAGC CTTGAAGATG GACCACGTGG CGCACGTCCC CCAGTCTCCA 
GCCAGCCACG TGGGGGGGCG TCCGCCGCAG GAGGAGACCA GCGCAGACAT GCTGCGGCCA 
GATCCCAGGG ATACCTTTTT CCTCACTCCA CGCATGGAAT CTTCGAGCCT GGAGAACGTG 
CTGGAGCCCT GCGCCTACGC CCCCACCTAC GTGGTCAAGG ACTTCCCGAT CGCCAGATAC 
CAGGGCCTGC AATTTGTGTA CCTGTCCTTC GTTTATCCCA ACGACTACAC TCGCCTCACC 
CACATGGAGA CGGACAACAA GTGCTTCTAC CGCGAGTCTC CGCTGTATCT GGAGAGGTTT 
GGGTTCTATA AATACATGAA GATGGACAAG GAGGAGGGGG ATGAGGATGA AGAAGACGAG 
GTGCAGCGCC GAGCCTTCCT CTTCCTCAAC CCGGACGACT TCCTGGACGA CGAGGACGAG 
GGGGAGCTGC TCGACAGCCT GGAGCCCACC GAGGCGGCCC CGCCCAGGAG CGGCCCCCAG 
TCCCCCGCCC CAGCAGCCCC CGCCCAGCCC GGAGCCACCC TCGCCCCGCC GACCCCTCCC 
CGCCCCCGGG ACGGGGGGAC CCCCAGGCAC TCCCGGGCCC TGAGCTGGGC CGCCAGGGCC 
GCCCGCCCTT TGCCGCTCTT CTTGGGCCGA GCTCCGCCCC CGCGCCCTGC AGTGGAGCAG 
CCGCCCCCAA AGGTGTACGT GACCAGGGTG CGGCCGGGAC AGCGGGCATC CCCCCGGGCC 
CCAGCGCCGC GTGCGCCCTG GCCGCCCTTC CCTGGCGTCT TCCTGCACCC CAGGCCTCTG 
CCCAGAGTGC AGCTGCGGGC GCCCCCACGC CCACCCCGGC CCCACGGCCG CAGGACCGGC 
GGCCCCCAGG CCACACAGCC GAGGCCCCCA GCCCGGGCGC AGGCCACCCA AGGGGGCCGG 
GAGGGCCAGG CGCGCACGCT GGGACCTGCG GCGCCCACAG TGGACTCAAA CTTGTCCTCC 
GAAGCGCGGC CCGTGACCTC CTTCCTGAGC TTGTCCCAGG TGTCCGGGCC GCAGCTGCCC 
GGGGAGGGCG AAGAGGAGGA GGAAGGGGAG GACGATGGGG CCCCGGGCGA CGAGGCCGCG 
TCGGAGGACA GCGAGGAGGC CGCGGGCCCG GCGCTCGGAC GCTGGCGTGA GGACGCCATC 
GACTGGCAGC GCACGTTCAG CGTGGGCGCC GTGGACTTCG AGCTGCTGCG CTCGGACTGG 
AACGACCTGC GATGCAACGT TTCGGGGAAC CTGCAGCTGC CGGAGGCGGA GGCCGTGGAC 
GTGACCGCTC AGTACATGGA GCGGCTGAAC GCGCGCCACG GCGGGCGCTT CGCGCTTCTG 
CGCATCGTGA ACGTGGAGAA GCGCCGGGAC TCGGCGCGAG GGAGTCGCTT CCTGCTGGAG 
CTGGAGCTGC AGGAGCGCGG GGGCGGCCGC CTGCGACTGT CCGAGTACGT CTTCCTGCGG 
CTGCCGGGAG CCCGCGTAGG GGATGCAGAC GGAGAAAGTC CCGAACCCGC TCCCGCCGCC 
TCCGTGCGCC CCGACGGCCG CCCCGAGCTC TGCCGGCCAC TGCGCCTGGC CTGGCGCCAG 
GACGTGATGG TTCACTTCAT CGTGCCAGTG AAAAACCAGG CACGGTGGGT GGCACAGTTC 
CTGGCGGACA TGGCTGCGCT GCACGCGCGC ACCGGGGACT CGCGTTTCAG CGTCGTCCTG 
GTGGATTTCG AGAGCGAGGA TATGGACGTG GAGCGGGCCC TGCGCGCCGC GCGCCTGCCC 
CGGTACCAGT ACCTGAGACG AACCGGGAAC TTCGAGCGCT CCGCCGGGCT GCAGGCGGGA 
GTGGACGCGG TAGAGGACGC CAGCAGCATC GTGTTCCTCT GCGACCTGCA CATCCACTTC 
CCACCCAACA TCCTGGACGG CATCCGCAAG CACTGCGTGG AGGGCAGGCT GGCCTTCGCG 
CCCGTGGTCA TGCGCCTGAG CTGCGGGAGC TCGCCCCGGG ACCCCCACGG TTACTGGGAG 
GTGAACGGCT TTGGCCTTTT TGGGATCTAC AAGTCGGACT TTGACCGGGT TGGAGGAATG 
AACACGGAGG AGTTCCGAGA CCAGTGGGGG GGTGAAGACT GGGAGCTCCT GGACAGGGTC 
CTGCAGGCAG GGCTGGAGGT GGAGCGGCTC CGACTGCGGA ATTTCTATCA CCACTACCAC 
TCCAAGAGGG GCATGTGGAG CGTCCGCAGC AGGAAGGGCT CTCGCACGGG GGCGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848632.2
CDS	306..3425
Translation	MPRLPVKKIRKQMKLLLLLLLLSCAAWLTYVHLGLVRQGRALRQRLGYGRDGEKLTSETDGRGVHAAPSTQRAEDSSESREEEQAPEGRDLDMLFPGGAGRLPLNFTHQTPPWREEYKGQVNLHVFEDWCGGAVGHLRRNLHFPLFPHTRTTVKKLAVSPKWKNYGLRIFGFIHPARDGDVQFSVASDDNSEFWLSLDESPAAAQLVAFVGKTGSEWTAPGEFTKFSSQVSKPRRLMASRRYYFELLHKQDDRGSDHVEVGWRAFLPGLKFEVISSAHISLYTDESALKMDHVAHVPQSPASHVGGRPPQEETSADMLRPDPRDTFFLTPRMESSSLENVLEPCAYAPTYVVKDFPIARYQGLQFVYLSFVYPNDYTRLTHMETDNKCFYRESPLYLERFGFYKYMKMDKEEGDEDEEDEVQRRAFLFLNPDDFLDDEDEGELLDSLEPTEAAPPRSGPQSPAPAAPAQPGATLAPPTPPRPRDGGTPRHSRALSWAARAARPLPLFLGRAPPPRPAVEQPPPKVYVTRVRPGQRASPRAPAPRAPWPPFPGVFLHPRPLPRVQLRAPPRPPRPHGRRTGGPQATQPRPPARAQATQGGREGQARTLGPAAPTVDSNLSSEARPVTSFLSLSQVSGPQLPGEGEEEEEGEDDGAPGDEAASEDSEEAAGPALGRWREDAIDWQRTFSVGAVDFELLRSDWNDLRCNVSGNLQLPEAEAVDVTAQYMERLNARHGGRFALLRIVNVEKRRDSARGSRFLLELELQERGGGRLRLSEYVFLRLPGARVGDADGESPEPAPAASVRPDGRPELCRPLRLAWRQDVMVHFIVPVKNQARWVAQFLADMAALHARTGDSRFSVVLVDFESEDMDVERALRAARLPRYQYLRRTGNFERSAGLQAGVDAVEDASSIVFLCDLHIHFPPNILDGIRKHCVEGRLAFAPVVMRLSCGSSPRDPHGYWEVNGFGLFGIYKSDFDRVGGMNTEEFRDQWGGEDWELLDRVLQAGLEVERLRLRNFYHHYHSKRGMWSVRSRKGSRTGAS

Target ORF information:

RefSeq Version	NM_178537.5
Organism	Homo sapiens(human)
Definition	Homo sapiens beta-1,4-N-acetyl-galactosaminyltransferase 4 (B4GALNT4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178537.5

ORF Insert Sequence:

ATGCCGCGGC TCCCGGTGAA GAAGATCCGT AAGCAGATGA AGCTGCTGCT GCTGCTGCTG 
CTGCTGAGCT GCGCCGCGTG GCTCACCTAC GTGCACCTGG GCCTGGTGCG CCAGGGACGC 
GCGCTGCGCC AGCGCCTGGG CTACGGGCGA GATGGTGAGA AGCTGACCAG TGAGACCGAC 
GGCCGGGGGG TCCACGCTGC GCCATCCACA CAGAGGGCTG AGGACTCCAG TGAGAGCCGT 
GAAGAGGAGC AAGCGCCCGA AGGTCGGGAC CTAGACATGC TGTTTCCTGG GGGGGCTGGG 
AGGCTGCCAC TGAACTTCAC CCATCAGACA CCCCCATGGC GGGAGGAGTA CAAGGGGCAG 
GTGAACCTGC ACGTGTTTGA GGACTGGTGT GGGGGCGCCG TGGGCCACCT GAGGAGGAAC 
CTGCACTTCC CGCTGTTCCC TCATACGCGC ACCACCGTGA AGAAGTTGGC CGTGTCCCCC 
AAGTGGAAGA ACTATGGACT CCGTATTTTT GGTTTCATCC ACCCGGCGAG GGACGGAGAC 
GTCCAGTTTT CTGTGGCCTC AGACGACAAC TCGGAGTTCT GGCTGAGTCT GGACGAGAGC 
CCTGCTGCTG CCCAGCTTGT GGCCTTTGTG GGCAAGACTG GCTCCGAGTG GACAGCGCCT 
GGAGAATTCA CCAAGTTCAG CTCCCAGGTG TCCAAGCCCA GGCGGCTCAT GGCCTCCCGG 
AGGTACTACT TTGAGTTGCT GCACAAGCAG GACGACCGCG GCTCGGACCA CGTGGAAGTG 
GGCTGGCGAG CTTTCCTGCC CGGCCTGAAG TTCGAGGTCA TCAGCTCTGC TCACATCTCC 
CTGTACACAG ATGAGTCAGC CTTGAAGATG GACCACGTGG CGCACGTCCC CCAGTCTCCA 
GCCAGCCACG TGGGGGGGCG TCCGCCGCAG GAGGAGACCA GCGCAGACAT GCTGCGGCCA 
GATCCCAGGG ATACCTTTTT CCTCACTCCA CGCATGGAAT CTTCGAGCCT GGAGAACGTG 
CTGGAGCCCT GCGCCTACGC CCCCACCTAC GTGGTCAAGG ACTTCCCGAT CGCCAGATAC 
CAGGGCCTGC AATTTGTGTA CCTGTCCTTC GTTTATCCCA ACGACTACAC TCGCCTCACC 
CACATGGAGA CGGACAACAA GTGCTTCTAC CGCGAGTCTC CGCTGTATCT GGAGAGGTTT 
GGGTTCTATA AATACATGAA GATGGACAAG GAGGAGGGGG ATGAGGATGA AGAAGACGAG 
GTGCAGCGCC GAGCCTTCCT CTTCCTCAAC CCGGACGACT TCCTGGACGA CGAGGACGAG 
GGGGAGCTGC TCGACAGCCT GGAGCCCACC GAGGCGGCCC CGCCCAGGAG CGGCCCCCAG 
TCCCCCGCCC CAGCAGCCCC CGCCCAGCCC GGAGCCACCC TCGCCCCGCC GACCCCTCCC 
CGCCCCCGGG ACGGGGGGAC CCCCAGGCAC TCCCGGGCCC TGAGCTGGGC CGCCAGGGCC 
GCCCGCCCTT TGCCGCTCTT CTTGGGCCGA GCTCCGCCCC CGCGCCCTGC AGTGGAGCAG 
CCGCCCCCAA AGGTGTACGT GACCAGGGTG CGGCCGGGAC AGCGGGCATC CCCCCGGGCC 
CCAGCGCCGC GTGCGCCCTG GCCGCCCTTC CCTGGCGTCT TCCTGCACCC CAGGCCTCTG 
CCCAGAGTGC AGCTGCGGGC GCCCCCACGC CCACCCCGGC CCCACGGCCG CAGGACCGGC 
GGCCCCCAGG CCACACAGCC GAGGCCCCCA GCCCGGGCGC AGGCCACCCA AGGGGGCCGG 
GAGGGCCAGG CGCGCACGCT GGGACCTGCG GCGCCCACAG TGGACTCAAA CTTGTCCTCC 
GAAGCGCGGC CCGTGACCTC CTTCCTGAGC TTGTCCCAGG TGTCCGGGCC GCAGCTGCCC 
GGGGAGGGCG AAGAGGAGGA GGAAGGGGAG GACGATGGGG CCCCGGGCGA CGAGGCCGCG 
TCGGAGGACA GCGAGGAGGC CGCGGGCCCG GCGCTCGGAC GCTGGCGTGA GGACGCCATC 
GACTGGCAGC GCACGTTCAG CGTGGGCGCC GTGGACTTCG AGCTGCTGCG CTCGGACTGG 
AACGACCTGC GATGCAACGT TTCGGGGAAC CTGCAGCTGC CGGAGGCGGA GGCCGTGGAC 
GTGACCGCTC AGTACATGGA GCGGCTGAAC GCGCGCCACG GCGGGCGCTT CGCGCTTCTG 
CGCATCGTGA ACGTGGAGAA GCGCCGGGAC TCGGCGCGAG GGAGTCGCTT CCTGCTGGAG 
CTGGAGCTGC AGGAGCGCGG GGGCGGCCGC CTGCGACTGT CCGAGTACGT CTTCCTGCGG 
CTGCCGGGAG CCCGCGTAGG GGATGCAGAC GGAGAAAGTC CCGAACCCGC TCCCGCCGCC 
TCCGTGCGCC CCGACGGCCG CCCCGAGCTC TGCCGGCCAC TGCGCCTGGC CTGGCGCCAG 
GACGTGATGG TTCACTTCAT CGTGCCAGTG AAAAACCAGG CACGGTGGGT GGCACAGTTC 
CTGGCGGACA TGGCTGCGCT GCACGCGCGC ACCGGGGACT CGCGTTTCAG CGTCGTCCTG 
GTGGATTTCG AGAGCGAGGA TATGGACGTG GAGCGGGCCC TGCGCGCCGC GCGCCTGCCC 
CGGTACCAGT ACCTGAGACG AACCGGGAAC TTCGAGCGCT CCGCCGGGCT GCAGGCGGGA 
GTGGACGCGG TAGAGGACGC CAGCAGCATC GTGTTCCTCT GCGACCTGCA CATCCACTTC 
CCACCCAACA TCCTGGACGG CATCCGCAAG CACTGCGTGG AGGGCAGGCT GGCCTTCGCG 
CCCGTGGTCA TGCGCCTGAG CTGCGGGAGC TCGCCCCGGG ACCCCCACGG TTACTGGGAG 
GTGAACGGCT TTGGCCTTTT TGGGATCTAC AAGTCGGACT TTGACCGGGT TGGAGGAATG 
AACACGGAGG AGTTCCGAGA CCAGTGGGGG GGTGAAGACT GGGAGCTCCT GGACAGGGTC 
CTGCAGGCAG GGCTGGAGGT GGAGCGGCTC CGACTGCGGA ATTTCTATCA CCACTACCAC 
TCCAAGAGGG GCATGTGGAG CGTCCGCAGC AGGAAGGGCT CTCGCACGGG GGCGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B4GALNT4 ( XM_017017654.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873143.1 Homo sapiens beta-1,4-N-acetyl-galactosaminyltransferase 4 (B4GALNT4), transcript variant X1, mRNA.

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CloneID	OHu92187
Clone ID Related Accession (Same CDS sequence)	XM_017017654.1
Accession Version	XM_017017654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTTC CTGGGGGGGC TGGGAGGCTG CCACTGAACT TCACCCATCA GACACCCCCA 
TGGCGGGAGG AGTACAAGGG GCAGGTGAAC CTGCACGTGT TTGAGGACTG GTGTGGGGGC 
GCCGTGGGCC ACCTGAGGAG GAACCTGCAC TTCCCGCTGT TCCCTCATAC GCGCACCACC 
GTGAAGAAGT TGGCCGTGTC CCCCAAGTGG AAGAACTATG GACTCCGTAT TTTTGGTTTC 
ATCCACCCGG CGAGGGACGG AGACGTCCAG TTTTCTGTGG CCTCAGACGA CAACTCGGAG 
TTCTGGCTGA GTCTGGACGA GAGCCCTGCT GCTGCCCAGC TTGTGGCCTT TGTGGGCAAG 
ACTGGCTCCG AGTGGACAGC GCCTGGAGAA TTCACCAAGT TCAGCTCCCA GGTGTCCAAG 
CCCAGGCGGC TCATGGCCTC CCGGAGGTAC TACTTTGAGT TGCTGCACAA GCAGGACGAC 
CGCGGCTCGG ACCACGTGGA AGTGGGCTGG CGAGCTTTCC TGCCCGGCCT GAAGTTCGAG 
GTCATCAGCT CTGCTCACAT CTCCCTGTAC ACAGATGAGT CAGCCTTGAA GATGGACCAC 
GTGGCGCACG TCCCCCAGTC TCCAGCCAGC CACGTGGGGG GGCGTCCGCC GCAGGAGGAG 
ACCAGCGCAG ACATGCTGCG GCCAGATCCC AGGGATACCT TTTTCCTCAC TCCACGCATG 
GAATCTTCGA GCCTGGAGAA CGTGCTGGAG CCCTGCGCCT ACGCCCCCAC CTACGTGGTC 
AAGGACTTCC CGATCGCCAG ATACCAGGGC CTGCAATTTG TGTACCTGTC CTTCGTTTAT 
CCCAACGACT ACACTCGCCT CACCCACATG GAGACGGACA ACAAGTGCTT CTACCGCGAG 
TCTCCGCTGT ATCTGGAGAG GTTTGGGTTC TATAAATACA TGAAGATGGA CAAGGAGGAG 
GGGGATGAGG ATGAAGAAGA CGAGGTGCAG CGCCGAGCCT TCCTCTTCCT CAACCCGGAC 
GACTTCCTGG ACGACGAGGA CGAGGGGGAG CTGCTCGACA GCCTGGAGCC CACCGAGGCG 
GCCCCGCCCA GGAGCGGCCC CCAGTCCCCC GCCCCAGCAG CCCCCGCCCA GCCCGGAGCC 
ACCCTCGCCC CGCCGACCCC TCCCCGCCCC CGGGACGGGG GGACCCCCAG GCACTCCCGG 
GCCCTGAGCT GGGCCGCCAG GGCCGCCCGC CCTTTGCCGC TCTTCTTGGG CCGAGCTCCG 
CCCCCGCGCC CTGCAGTGGA GCAGCCGCCC CCAAAGGTGT ACGTGACCAG GGTGCGGCCG 
GGACAGCGGG CATCCCCCCG GGCCCCAGCG CCGCGTGCGC CCTGGCCGCC CTTCCCTGGC 
GTCTTCCTGC ACCCCAGGCC TCTGCCCAGA GTGCAGCTGC GGGCGCCCCC ACGCCCACCC 
CGGCCCCACG GCCGCAGGAC CGGCGGCCCC CAGGCCACAC AGCCGAGGCC CCCAGCCCGG 
GCGCAGGCCA CCCAAGGGGG CCGGGAGGGC CAGGCGCGCA CGCTGGGACC TGCGGCGCCC 
ACAGTGGACT CAAACTTGTC CTCCGAAGCG CGGCCCGTGA CCTCCTTCCT GAGCTTGTCC 
CAGGTGTCCG GGCCGCAGCT GCCCGGGGAG GGCGAAGAGG AGGAGGAAGG GGAGGACGAT 
GGGGCCCCGG GCGACGAGGC CGCGTCGGAG GACAGCGAGG AGGCCGCGGG CCCGGCGCTC 
GGACGCTGGC GTGAGGACGC CATCGACTGG CAGCGCACGT TCAGCGTGGG CGCCGTGGAC 
TTCGAGCTGC TGCGCTCGGA CTGGAACGAC CTGCGATGCA ACGTTTCGGG GAACCTGCAG 
CTGCCGGAGG CGGAGGCCGT GGACGTGACC GCTCAGTACA TGGAGCGGCT GAACGCGCGC 
CACGGCGGGC GCTTCGCGCT TCTGCGCATC GTGAACGTGG AGAAGCGCCG GGACTCGGCG 
CGAGGGAGTC GCTTCCTGCT GGAGCTGGAG CTGCAGGAGC GCGGGGGCGG CCGCCTGCGA 
CTGTCCGAGT ACGTCTTCCT GCGGCTGCCG GGAGCCCGCG TAGGGGATGC AGACGGAGAA 
AGTCCCGAAC CCGCTCCCGC CGCCTCCGTG CGCCCCGACG GCCGCCCCGA GCTCTGCCGG 
CCACTGCGCC TGGCCTGGCG CCAGGACGTG ATGGTTCACT TCATCGTGCC AGTGAAAAAC 
CAGGCACGGT GGGTGGCACA GTTCCTGGCG GACATGGCTG CGCTGCACGC GCGCACCGGG 
GACTCGCGTT TCAGCGTCGT CCTGGTGGAT TTCGAGAGCG AGGATATGGA CGTGGAGCGG 
GCCCTGCGCG CCGCGCGCCT GCCCCGGTAC CAGTACCTGA GACGAACCGG GAACTTCGAG 
CGCTCCGCCG GGCTGCAGGC GGGAGTGGAC GCGGTAGAGG ACGCCAGCAG CATCGTGTTC 
CTCTGCGACC TGCACATCCA CTTCCCACCC AACATCCTGG ACGGCATCCG CAAGCACTGC 
GTGGAGGGCA GGCTGGCCTT CGCGCCCGTG GTCATGCGCC TGAGCTGCGG GAGCTCGCCC 
CGGGACCCCC ACGGTTACTG GGAGGTGAAC GGCTTTGGCC TTTTTGGGAT CTACAAGTCG 
GACTTTGACC GGGTTGGAGG AATGAACACG GAGGAGTTCC GAGACCAGTG GGGGGGTGAA 
GACTGGGAGC TCCTGGACAG GGTCCTGCAG GCAGGGCTGG AGGTGGAGCG GCTCCGACTG 
CGGAATTTCT ATCACCACTA CCACTCCAAG AGGGGCATGT GGAGCGTCCG CAGCAGGAAG 
GGCTCTCGCA CGGGGGCGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873143.1
CDS	1598..4441
Translation	MLFPGGAGRLPLNFTHQTPPWREEYKGQVNLHVFEDWCGGAVGHLRRNLHFPLFPHTRTTVKKLAVSPKWKNYGLRIFGFIHPARDGDVQFSVASDDNSEFWLSLDESPAAAQLVAFVGKTGSEWTAPGEFTKFSSQVSKPRRLMASRRYYFELLHKQDDRGSDHVEVGWRAFLPGLKFEVISSAHISLYTDESALKMDHVAHVPQSPASHVGGRPPQEETSADMLRPDPRDTFFLTPRMESSSLENVLEPCAYAPTYVVKDFPIARYQGLQFVYLSFVYPNDYTRLTHMETDNKCFYRESPLYLERFGFYKYMKMDKEEGDEDEEDEVQRRAFLFLNPDDFLDDEDEGELLDSLEPTEAAPPRSGPQSPAPAAPAQPGATLAPPTPPRPRDGGTPRHSRALSWAARAARPLPLFLGRAPPPRPAVEQPPPKVYVTRVRPGQRASPRAPAPRAPWPPFPGVFLHPRPLPRVQLRAPPRPPRPHGRRTGGPQATQPRPPARAQATQGGREGQARTLGPAAPTVDSNLSSEARPVTSFLSLSQVSGPQLPGEGEEEEEGEDDGAPGDEAASEDSEEAAGPALGRWREDAIDWQRTFSVGAVDFELLRSDWNDLRCNVSGNLQLPEAEAVDVTAQYMERLNARHGGRFALLRIVNVEKRRDSARGSRFLLELELQERGGGRLRLSEYVFLRLPGARVGDADGESPEPAPAASVRPDGRPELCRPLRLAWRQDVMVHFIVPVKNQARWVAQFLADMAALHARTGDSRFSVVLVDFESEDMDVERALRAARLPRYQYLRRTGNFERSAGLQAGVDAVEDASSIVFLCDLHIHFPPNILDGIRKHCVEGRLAFAPVVMRLSCGSSPRDPHGYWEVNGFGLFGIYKSDFDRVGGMNTEEFRDQWGGEDWELLDRVLQAGLEVERLRLRNFYHHYHSKRGMWSVRSRKGSRTGAS

Target ORF information:

RefSeq Version	XM_017017654.1
Organism	Homo sapiens(human)
Definition	Homo sapiens beta-1,4-N-acetyl-galactosaminyltransferase 4 (B4GALNT4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017654.1

ORF Insert Sequence:

ATGCTGTTTC CTGGGGGGGC TGGGAGGCTG CCACTGAACT TCACCCATCA GACACCCCCA 
TGGCGGGAGG AGTACAAGGG GCAGGTGAAC CTGCACGTGT TTGAGGACTG GTGTGGGGGC 
GCCGTGGGCC ACCTGAGGAG GAACCTGCAC TTCCCGCTGT TCCCTCATAC GCGCACCACC 
GTGAAGAAGT TGGCCGTGTC CCCCAAGTGG AAGAACTATG GACTCCGTAT TTTTGGTTTC 
ATCCACCCGG CGAGGGACGG AGACGTCCAG TTTTCTGTGG CCTCAGACGA CAACTCGGAG 
TTCTGGCTGA GTCTGGACGA GAGCCCTGCT GCTGCCCAGC TTGTGGCCTT TGTGGGCAAG 
ACTGGCTCCG AGTGGACAGC GCCTGGAGAA TTCACCAAGT TCAGCTCCCA GGTGTCCAAG 
CCCAGGCGGC TCATGGCCTC CCGGAGGTAC TACTTTGAGT TGCTGCACAA GCAGGACGAC 
CGCGGCTCGG ACCACGTGGA AGTGGGCTGG CGAGCTTTCC TGCCCGGCCT GAAGTTCGAG 
GTCATCAGCT CTGCTCACAT CTCCCTGTAC ACAGATGAGT CAGCCTTGAA GATGGACCAC 
GTGGCGCACG TCCCCCAGTC TCCAGCCAGC CACGTGGGGG GGCGTCCGCC GCAGGAGGAG 
ACCAGCGCAG ACATGCTGCG GCCAGATCCC AGGGATACCT TTTTCCTCAC TCCACGCATG 
GAATCTTCGA GCCTGGAGAA CGTGCTGGAG CCCTGCGCCT ACGCCCCCAC CTACGTGGTC 
AAGGACTTCC CGATCGCCAG ATACCAGGGC CTGCAATTTG TGTACCTGTC CTTCGTTTAT 
CCCAACGACT ACACTCGCCT CACCCACATG GAGACGGACA ACAAGTGCTT CTACCGCGAG 
TCTCCGCTGT ATCTGGAGAG GTTTGGGTTC TATAAATACA TGAAGATGGA CAAGGAGGAG 
GGGGATGAGG ATGAAGAAGA CGAGGTGCAG CGCCGAGCCT TCCTCTTCCT CAACCCGGAC 
GACTTCCTGG ACGACGAGGA CGAGGGGGAG CTGCTCGACA GCCTGGAGCC CACCGAGGCG 
GCCCCGCCCA GGAGCGGCCC CCAGTCCCCC GCCCCAGCAG CCCCCGCCCA GCCCGGAGCC 
ACCCTCGCCC CGCCGACCCC TCCCCGCCCC CGGGACGGGG GGACCCCCAG GCACTCCCGG 
GCCCTGAGCT GGGCCGCCAG GGCCGCCCGC CCTTTGCCGC TCTTCTTGGG CCGAGCTCCG 
CCCCCGCGCC CTGCAGTGGA GCAGCCGCCC CCAAAGGTGT ACGTGACCAG GGTGCGGCCG 
GGACAGCGGG CATCCCCCCG GGCCCCAGCG CCGCGTGCGC CCTGGCCGCC CTTCCCTGGC 
GTCTTCCTGC ACCCCAGGCC TCTGCCCAGA GTGCAGCTGC GGGCGCCCCC ACGCCCACCC 
CGGCCCCACG GCCGCAGGAC CGGCGGCCCC CAGGCCACAC AGCCGAGGCC CCCAGCCCGG 
GCGCAGGCCA CCCAAGGGGG CCGGGAGGGC CAGGCGCGCA CGCTGGGACC TGCGGCGCCC 
ACAGTGGACT CAAACTTGTC CTCCGAAGCG CGGCCCGTGA CCTCCTTCCT GAGCTTGTCC 
CAGGTGTCCG GGCCGCAGCT GCCCGGGGAG GGCGAAGAGG AGGAGGAAGG GGAGGACGAT 
GGGGCCCCGG GCGACGAGGC CGCGTCGGAG GACAGCGAGG AGGCCGCGGG CCCGGCGCTC 
GGACGCTGGC GTGAGGACGC CATCGACTGG CAGCGCACGT TCAGCGTGGG CGCCGTGGAC 
TTCGAGCTGC TGCGCTCGGA CTGGAACGAC CTGCGATGCA ACGTTTCGGG GAACCTGCAG 
CTGCCGGAGG CGGAGGCCGT GGACGTGACC GCTCAGTACA TGGAGCGGCT GAACGCGCGC 
CACGGCGGGC GCTTCGCGCT TCTGCGCATC GTGAACGTGG AGAAGCGCCG GGACTCGGCG 
CGAGGGAGTC GCTTCCTGCT GGAGCTGGAG CTGCAGGAGC GCGGGGGCGG CCGCCTGCGA 
CTGTCCGAGT ACGTCTTCCT GCGGCTGCCG GGAGCCCGCG TAGGGGATGC AGACGGAGAA 
AGTCCCGAAC CCGCTCCCGC CGCCTCCGTG CGCCCCGACG GCCGCCCCGA GCTCTGCCGG 
CCACTGCGCC TGGCCTGGCG CCAGGACGTG ATGGTTCACT TCATCGTGCC AGTGAAAAAC 
CAGGCACGGT GGGTGGCACA GTTCCTGGCG GACATGGCTG CGCTGCACGC GCGCACCGGG 
GACTCGCGTT TCAGCGTCGT CCTGGTGGAT TTCGAGAGCG AGGATATGGA CGTGGAGCGG 
GCCCTGCGCG CCGCGCGCCT GCCCCGGTAC CAGTACCTGA GACGAACCGG GAACTTCGAG 
CGCTCCGCCG GGCTGCAGGC GGGAGTGGAC GCGGTAGAGG ACGCCAGCAG CATCGTGTTC 
CTCTGCGACC TGCACATCCA CTTCCCACCC AACATCCTGG ACGGCATCCG CAAGCACTGC 
GTGGAGGGCA GGCTGGCCTT CGCGCCCGTG GTCATGCGCC TGAGCTGCGG GAGCTCGCCC 
CGGGACCCCC ACGGTTACTG GGAGGTGAAC GGCTTTGGCC TTTTTGGGAT CTACAAGTCG 
GACTTTGACC GGGTTGGAGG AATGAACACG GAGGAGTTCC GAGACCAGTG GGGGGGTGAA 
GACTGGGAGC TCCTGGACAG GGTCCTGCAG GCAGGGCTGG AGGTGGAGCG GCTCCGACTG 
CGGAATTTCT ATCACCACTA CCACTCCAAG AGGGGCATGT GGAGCGTCCG CAGCAGGAAG 
GGCTCTCGCA CGGGGGCGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Enhanced expression of the ?4-N-acetylgalactosaminyltransferase 4?gene impairs tumor growth of human breast cancer cells.
Biochemical and biophysical research communications461(1)80-5(2015 May)
Hirano K,Matsuda A,Kuji R,Nakandakari S,Shirai T,Furukawa K

Molecular basis for protein-specific transfer of N-acetylgalactosamine to N-linked glycans by the glycosyltransferases ?1,4-N-acetylgalactosaminyl transferase 3 (?4GalNAc-T3) and ?4GalNAc-T4.
The Journal of biological chemistry287(34)29194-203(2012 Aug)
Fiete D,Beranek M,Baenziger JU

Peptide-specific transfer of N-acetylgalactosamine to O-linked glycans by the glycosyltransferases ?1,4-N-acetylgalactosaminyl transferase 3 (?4GalNAc-T3) and ?4GalNAc-T4.
The Journal of biological chemistry287(34)29204-12(2012 Aug)
Fiete D,Beranek M,Baenziger JU

A necessary and sufficient determinant for protein-selective glycosylation in vivo.
The Journal of biological chemistry283(4)1985-91(2008 Jan)
Miller E,Fiete D,Blake NM,Beranek M,Oates EL,Mi Y,Roseman DS,Baenziger JU

Molecular cloning and characterization of beta1,4-N-acetylgalactosaminyltransferases IV synthesizing N,N'-diacetyllactosediamine.
FEBS letters562(1-3)134-40(2004 Mar)
Gotoh M,Sato T,Kiyohara K,Kameyama A,Kikuchi N,Kwon YD,Ishizuka Y,Iwai T,Nakanishi H,Narimatsu H

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B4GALNT4 cDNA ORF clone, Homo sapiens(human)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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