KCNK18 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KCNK18
Entrez Gene ID	338567
Full Name	potassium two pore domain channel subfamily K member 18
Synonyms	K2p18.1,MGR13,TRESK,TRESK-2,TRESK2,TRIK
General protein information	Preferred Names potassium two pore domain channel subfamily K member 18 Names potassium channel subfamily K member 18 TWIK-related individual K+ channel TWIK-related individual potassium channel TWIK-related spinal cord K+ channel TWIK-related spinal cord potassium channel potassium channel, subfamily K, member 18 potassium channel, two pore domain subfamily K, member 18
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q25.3
Summary	Potassium channels play a role in many cellular processes including maintenance of the action potential, muscle contraction, hormone secretion, osmotic regulation, and ion flow. This gene encodes a member of the superfamily of potassium channel proteins containing two pore-forming P domains and the encoded protein functions as an outward rectifying potassium channel. A mutation in this gene has been found to be associated with migraine with aura.[provided by RefSeq, Jan 2011].
Disorder MIM:	613655
Disorder Html:	{Migraine, with or without aura, susceptibility to, 13}, 613656 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_181840.1	NP_862823.1	potassium channel subfamily K member 18

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Kcnk18	NP_001003820.1
Danio rerio (zebrafish)	kcnk18	NP_001177236.2
Homo sapiens (human)	KCNK18	NP_862823.1
Pan troglodytes (chimpanzee)	KCNK18	XP_521614.2
Mus musculus (house mouse)	Kcnk18	NP_997144.1
Gallus gallus (chicken)	KCNK18	XP_001234729.2
Xenopus tropicalis (tropical clawed frog)	LOC100486306	XP_002943434.2
	KCNK18	XP_001095493.1
Canis lupus familiaris (dog)	KCNK18	XP_003640047.1
Bos taurus (cattle)	KCNK18	XP_002698581.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic studies of Polish migraine patients: screening for causative mutations in four migraine-associated genes.
Domitrz I, Kosiorek M, ?ekanowski C, Kami?ska A
Human genomics103(2016 Jan)

The LQLP calcineurin docking site is a major determinant of the calcium-dependent activation of human TRESK background K+ channel.
Czirj?k G, Enyedi P
The Journal of biological chemistry289(43)29506-18(2014 Oct)

KCNK18 (TRESK) genetic variants in Italian patients with migraine.
Rainero I, Rubino E, Gallone S, Zavarise P, Carli D, Boschi S, Fenoglio P, Savi L, Gentile S, Benna P, Pinessi L, Dalla Volta G
Headache54(9)1515-22(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The presence of SCN1A mutations and absence of mutations in ATP1A2 or CACNA1A suggest that the Polish patients represent FHM type 3.
Title: Genetic studies of Polish migraine patients: screening for causative mutations in four migraine-associated genes.

LQLP site is a fundamental determinant of the calcium-sensitivity of human TRESK.
Title: The LQLP calcineurin docking site is a major determinant of the calcium-dependent activation of human TRESK background K+ channel.

This study reveals new pharmacological modulators of K2P18.1 activity useful in dissecting native K2P18.1 function
Title: Identification of novel small molecule modulators of K2P18.1 two-pore potassium channel.

Our study shows the presence of several KCNK18 gene mutations in both migraine with aura and migraine without aura
Title: KCNK18 (TRESK) genetic variants in Italian patients with migraine.

Migraine-associated TRESK mutation, but not the C110R variant, reduces the endogenous TRESK currents to a degree that affects trigeminal ganglion neuron excitability
Title: Nonmigraine-associated TRESK K+ channel variant C110R does not increase the excitability of trigeminal ganglion neurons.

The following KCNK18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KCNK18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu17962	NM_181840.1 Latest version!	Homo sapiens potassium two pore domain channel subfamily K member 18 (KCNK18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KCNK18 ( NM_181840.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_862823.1 Homo sapiens potassium two pore domain channel subfamily K member 18 (KCNK18), mRNA.

Price & Availability↑

CloneID	OHu17962
Clone ID Related Accession (Same CDS sequence)	NM_181840.1
Accession Version	NM_181840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	potassium channel subfamily K member 18
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB087138.1. This sequence is a reference standard in the RefSeqGene project. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB087138.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2145743, SAMEA2151119 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000334549.1/ ENSP00000334650.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGAGGTCT CGGGGCACCC CCAGGCCAGG AGATGCTGCC CAGAGGCCCT GGGAAAGCTC 
TTCCCTGGCC TCTGCTTCCT CTGCTTTCTG GTGACCTACG CCCTGGTGGG TGCTGTGGTC 
TTCTCTGCCA TTGAGGACGG CCAGGTCCTG GTGGCAGCAG ATGATGGAGA GTTTGAGAAG 
TTCTTGGAGG AGCTCTGCAG AATCTTGAAC TGCAGTGAAA CAGTGGTGGA AGACAGAAAA 
CAGGATCTCC AGGGGCATCT GCAGAAGGTG AAGCCTCAGT GGTTTAACAG GACCACACAC 
TGGTCCTTCC TGAGCTCGCT CTTTTTCTGC TGCACGGTGT TCAGCACCGT GGGCTATGGC 
TACATCTACC CCGTCACCAG GCTTGGCAAG TACTTGTGCA TGCTCTATGC TCTCTTTGGT 
ATCCCCCTGA TGTTCCTCGT TCTCACGGAC ACAGGCGACA TCCTGGCAAC CATCTTATCT 
ACATCTTATA ATCGGTTCCG AAAATTCCCT TTCTTTACCC GCCCCCTCCT CTCCAAGTGG 
TGCCCCAAAT CTCTCTTCAA GAAAAAACCG GACCCCAAGC CCGCAGATGA AGCTGTCCCT 
CAGATCATCA TCAGTGCTGA AGAGCTTCCA GGCCCCAAAC TTGGCACATG TCCTTCACGC 
CCAAGCTGCA GCATGGAGCT GTTTGAGAGA TCTCATGCGC TAGAGAAACA GAACACACTG 
CAACTGCCCC CACAAGCCAT GGAGAGGAGT AACTCGTGTC CCGAACTGGT GTTGGGAAGA 
CTCTCATACT CCATCATCAG CAACCTGGAT GAAGTTGGAC AGCAGGTGGA GAGGTTGGAC 
ATCCCCCTCC CCATCATTGC CCTTATTGTT TTTGCCTACA TTTCCTGTGC AGCTGCCATC 
CTCCCCTTCT GGGAGACACA GTTGGATTTC GAGAATGCCT TCTATTTCTG CTTTGTCACA 
CTCACCACCA TTGGGTTTGG GGATACTGTT TTAGAACACC CTAACTTCTT CCTGTTCTTC 
TCCATTTATA TCATCGTTGG AATGGAGATT GTGTTCATTG CTTTCAAGTT GGTGCAAAAC 
AGGCTGATTG ACATATACAA AAATGTTATG CTATTCTTTG CAAAAGGGAA GTTTTACCAC 
CTTGTTAAAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_862823.1
CDS	1..1155
Translation	MEVSGHPQARRCCPEALGKLFPGLCFLCFLVTYALVGAVVFSAIEDGQVLVAADDGEFEKFLEELCRILNCSETVVEDRKQDLQGHLQKVKPQWFNRTTHWSFLSSLFFCCTVFSTVGYGYIYPVTRLGKYLCMLYALFGIPLMFLVLTDTGDILATILSTSYNRFRKFPFFTRPLLSKWCPKSLFKKKPDPKPADEAVPQIIISAEELPGPKLGTCPSRPSCSMELFERSHALEKQNTLQLPPQAMERSNSCPELVLGRLSYSIISNLDEVGQQVERLDIPLPIIALIVFAYISCAAAILPFWETQLDFENAFYFCFVTLTTIGFGDTVLEHPNFFLFFSIYIIVGMEIVFIAFKLVQNRLIDIYKNVMLFFAKGKFYHLVKK

Target ORF information:

RefSeq Version	NM_181840.1
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium two pore domain channel subfamily K member 18 (KCNK18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181840.1

ORF Insert Sequence:

ATGGAGGTCT CGGGGCACCC CCAGGCCAGG AGATGCTGCC CAGAGGCCCT GGGAAAGCTC 
TTCCCTGGCC TCTGCTTCCT CTGCTTTCTG GTGACCTACG CCCTGGTGGG TGCTGTGGTC 
TTCTCTGCCA TTGAGGACGG CCAGGTCCTG GTGGCAGCAG ATGATGGAGA GTTTGAGAAG 
TTCTTGGAGG AGCTCTGCAG AATCTTGAAC TGCAGTGAAA CAGTGGTGGA AGACAGAAAA 
CAGGATCTCC AGGGGCATCT GCAGAAGGTG AAGCCTCAGT GGTTTAACAG GACCACACAC 
TGGTCCTTCC TGAGCTCGCT CTTTTTCTGC TGCACGGTGT TCAGCACCGT GGGCTATGGC 
TACATCTACC CCGTCACCAG GCTTGGCAAG TACTTGTGCA TGCTCTATGC TCTCTTTGGT 
ATCCCCCTGA TGTTCCTCGT TCTCACGGAC ACAGGCGACA TCCTGGCAAC CATCTTATCT 
ACATCTTATA ATCGGTTCCG AAAATTCCCT TTCTTTACCC GCCCCCTCCT CTCCAAGTGG 
TGCCCCAAAT CTCTCTTCAA GAAAAAACCG GACCCCAAGC CCGCAGATGA AGCTGTCCCT 
CAGATCATCA TCAGTGCTGA AGAGCTTCCA GGCCCCAAAC TTGGCACATG TCCTTCACGC 
CCAAGCTGCA GCATGGAGCT GTTTGAGAGA TCTCATGCGC TAGAGAAACA GAACACACTG 
CAACTGCCCC CACAAGCCAT GGAGAGGAGT AACTCGTGTC CCGAACTGGT GTTGGGAAGA 
CTCTCATACT CCATCATCAG CAACCTGGAT GAAGTTGGAC AGCAGGTGGA GAGGTTGGAC 
ATCCCCCTCC CCATCATTGC CCTTATTGTT TTTGCCTACA TTTCCTGTGC AGCTGCCATC 
CTCCCCTTCT GGGAGACACA GTTGGATTTC GAGAATGCCT TCTATTTCTG CTTTGTCACA 
CTCACCACCA TTGGGTTTGG GGATACTGTT TTAGAACACC CTAACTTCTT CCTGTTCTTC 
TCCATTTATA TCATCGTTGG AATGGAGATT GTGTTCATTG CTTTCAAGTT GGTGCAAAAC 
AGGCTGATTG ACATATACAA AAATGTTATG CTATTCTTTG CAAAAGGGAA GTTTTACCAC 
CTTGTTAAAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic studies of Polish migraine patients: screening for causative mutations in four migraine-associated genes.
Human genomics103(2016 Jan)
Domitrz I,Kosiorek M,?ekanowski C,Kami?ska A

The LQLP calcineurin docking site is a major determinant of the calcium-dependent activation of human TRESK background K+ channel.
The Journal of biological chemistry289(43)29506-18(2014 Oct)
Czirj?k G,Enyedi P

KCNK18 (TRESK) genetic variants in Italian patients with migraine.
Headache54(9)1515-22(2014 Oct)
Rainero I,Rubino E,Gallone S,Zavarise P,Carli D,Boschi S,Fenoglio P,Savi L,Gentile S,Benna P,Pinessi L,Dalla Volta G

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KCNK18 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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