HCAR2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HCAR2
Entrez Gene ID	338442
Full Name	hydroxycarboxylic acid receptor 2
Synonyms	GPR109A,HCA2,HM74a,HM74b,NIACR1,PUMAG,Puma-g
General protein information	Preferred Names hydroxycarboxylic acid receptor 2 Names hydroxycarboxylic acid receptor 2 G protein-coupled receptor HM74a G-protein coupled receptor 109A G-protein coupled receptor HM74A hydroxy-carboxylic acid receptor 2 niacin receptor 1 nicotinic acid receptor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.31
Disorder MIM:	609163

mRNA and Protein(s)

mRNA	Protein	Name
NM_177551.4	NP_808219.1	hydroxycarboxylic acid receptor 2
NM_177551.3	NP_808219.1	hydroxycarboxylic acid receptor 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	HCAR2	NP_808219.1
Mus musculus (house mouse)	Niacr1	NP_109626.1
Pan troglodytes (chimpanzee)	GPR109A	XP_003314054.1
Rattus norvegicus (Norway rat)	Niacr1	NP_852141.1

Gene Ontology
Bibliography

Related articles in PubMed

Nicotinic Acid Receptor GPR109A Exerts Anti-Inflammatory Effects Through Inhibiting the Akt/mTOR Signaling Pathway in MIN6 Pancreatic ? cells.
Li Z, Li X, Lin S, Chen Y, Ma S, Fu Y, Wei C, Xu W
Annals of clinical and laboratory science47(6)729-737(2017 Nov)

Niacin receptor GPR109A inhibits insulin secretion and is down-regulated in type 2 diabetic islet beta-cells.
Wang N, Guo DY, Tian X, Lin HP, Li YP, Chen SJ, Fu YC, Xu WC, Wei CJ
General and comparative endocrinology23798-108(2016 Oct)

The role of the C-terminus of the human hydroxycarboxylic acid receptors 2 and 3 in G protein activation using G?-engineered yeast cells.
Liu R, van Veldhoven JP, IJzerman AP
European journal of pharmacology77070-7(2016 Jan)

The atypical N-glycosylation motif, Asn-Cys-Cys, in human GPR109A is required for normal cell surface expression and intracellular signaling.
Yasuda D, Imura Y, Ishii S, Shimizu T, Nakamura M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology29(6)2412-22(2015 Jun)

Niacin receptor activation improves human microvascular endothelial cell angiogenic function during lipotoxicity.
Hughes-Large JM, Pang DK, Robson DL, Chan P, Toma J, Borradaile NM
Atherosclerosis237(2)696-704(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results demonstrate that GPR109A is functionally expressed in both human and murine islet beta-cells.
Title: Niacin receptor GPR109A inhibits insulin secretion and is down-regulated in type 2 diabetic islet beta-cells.

new insights into the G protein coupling profiles of the HCA receptors and the function of the receptor's C terminus
Title: The role of the C-terminus of the human hydroxycarboxylic acid receptors 2 and 3 in G protein activation using G?-engineered yeast cells.

Results suggest that the atypical motif asparaging-cysteine-systeine Asn(17)-Cys(18)-Cys(19) is crucial for the normal surface trafficking and function of hydroxycarboxylic acid receptor 2 protein hGPR109A.
Title: The atypical N-glycosylation motif, Asn-Cys-Cys, in human GPR109A is required for normal cell surface expression and intracellular signaling.

niacin, at a relatively low concentration, preserves the ability of HMVEC to form tubes under conditions of saturated fatty acid excess, and may elicit this effect through activation of GPR109A
Title: Niacin receptor activation improves human microvascular endothelial cell angiogenic function during lipotoxicity.

These results suggest that the PKC pathway and PDGFR/EGFR transactivation pathway play important roles in HCA2-mediated Akt activation.
Title: Niacin activates the PI3K/Akt cascade via PKC- and EGFR-transactivation-dependent pathways through hydroxyl-carboxylic acid receptor 2.

The following HCAR2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCAR2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17408	NM_177551.4 Latest version!	Homo sapiens hydroxycarboxylic acid receptor 2 (HCAR2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu17408	NM_177551.3	Homo sapiens hydroxycarboxylic acid receptor 2 (HCAR2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HCAR2 ( NM_177551.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_808219.1 Homo sapiens hydroxycarboxylic acid receptor 2 (HCAR2), mRNA.

Price & Availability↑

CloneID	OHu17408
Clone ID Related Accession (Same CDS sequence)	NM_177551.4 , NM_177551.3
Accession Version	NM_177551.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	hydroxycarboxylic acid receptor 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL698846.1, BC027965.1, BC056419.1, AL572062.2 and CD364466.1. On May 17, 2019 this sequence version replaced NM_177551.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC027965.1, DRR138517.935822.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000328880.6/ ENSP00000375066.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGAATCGGC ACCATCTGCA GGATCACTTT CTGGAAATAG ACAAGAAGAA CTGCTGTGTG 
TTCCGAGATG ACTTCATTGT CAAGGTGTTG CCGCCGGTGT TGGGGCTGGA GTTTATCTTC 
GGGCTTCTGG GCAATGGCCT TGCCCTGTGG ATTTTCTGTT TCCACCTCAA GTCCTGGAAA 
TCCAGCCGGA TTTTCCTGTT CAACCTGGCA GTGGCTGACT TTCTACTGAT CATCTGCCTG 
CCCTTCCTGA TGGACAACTA TGTGAGGCGT TGGGACTGGA AGTTTGGGGA CATCCCTTGC 
CGGCTGATGC TCTTCATGTT GGCTATGAAC CGCCAGGGCA GCATCATCTT CCTCACGGTG 
GTGGCGGTAG ACAGGTATTT CCGGGTGGTC CATCCCCACC ACGCCCTGAA CAAGATCTCC 
AATCGGACAG CAGCCATCAT CTCTTGCCTT CTGTGGGGCA TCACTATTGG CCTGACAGTC 
CACCTCCTGA AGAAGAAGAT GCCGATCCAG AATGGCGGTG CAAATTTGTG CAGCAGCTTC 
AGCATCTGCC ATACCTTCCA GTGGCACGAA GCCATGTTCC TCCTGGAGTT CTTCCTGCCC 
CTGGGCATCA TCCTGTTCTG CTCAGCCAGA ATTATCTGGA GCCTGCGGCA GAGACAAATG 
GACCGGCATG CCAAGATCAA GAGAGCCATC ACCTTCATCA TGGTGGTGGC CATCGTCTTT 
GTCATCTGCT TCCTTCCCAG CGTGGTTGTG CGGATCCGCA TCTTCTGGCT CCTGCACACT 
TCGGGCACGC AGAATTGTGA AGTGTACCGC TCGGTGGACC TGGCGTTCTT TATCACTCTC 
AGCTTCACCT ACATGAACAG CATGCTGGAC CCCGTGGTGT ACTACTTCTC CAGCCCATCC 
TTTCCCAACT TCTTCTCCAC TTTGATCAAC CGCTGCCTCC AGAGGAAGAT GACAGGTGAG 
CCAGATAATA ACCGCAGCAC GAGCGTCGAG CTCACAGGGG ACCCCAACAA AACCAGAGGC 
GCTCCAGAGG CGTTAATGGC CAACTCCGGT GAGCCATGGA GCCCCTCTTA TCTGGGCCCA 
ACCTCTCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808219.1
CDS	75..1166
Translation	MNRHHLQDHFLEIDKKNCCVFRDDFIVKVLPPVLGLEFIFGLLGNGLALWIFCFHLKSWKSSRIFLFNLAVADFLLIICLPFLMDNYVRRWDWKFGDIPCRLMLFMLAMNRQGSIIFLTVVAVDRYFRVVHPHHALNKISNRTAAIISCLLWGITIGLTVHLLKKKMPIQNGGANLCSSFSICHTFQWHEAMFLLEFFLPLGIILFCSARIIWSLRQRQMDRHAKIKRAITFIMVVAIVFVICFLPSVVVRIRIFWLLHTSGTQNCEVYRSVDLAFFITLSFTYMNSMLDPVVYYFSSPSFPNFFSTLINRCLQRKMTGEPDNNRSTSVELTGDPNKTRGAPEALMANSGEPWSPSYLGPTSP

Target ORF information:

RefSeq Version	NM_177551.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hydroxycarboxylic acid receptor 2 (HCAR2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177551.4

ORF Insert Sequence:

ATGAATCGGC ACCATCTGCA GGATCACTTT CTGGAAATAG ACAAGAAGAA CTGCTGTGTG 
TTCCGAGATG ACTTCATTGT CAAGGTGTTG CCGCCGGTGT TGGGGCTGGA GTTTATCTTC 
GGGCTTCTGG GCAATGGCCT TGCCCTGTGG ATTTTCTGTT TCCACCTCAA GTCCTGGAAA 
TCCAGCCGGA TTTTCCTGTT CAACCTGGCA GTGGCTGACT TTCTACTGAT CATCTGCCTG 
CCCTTCCTGA TGGACAACTA TGTGAGGCGT TGGGACTGGA AGTTTGGGGA CATCCCTTGC 
CGGCTGATGC TCTTCATGTT GGCTATGAAC CGCCAGGGCA GCATCATCTT CCTCACGGTG 
GTGGCGGTAG ACAGGTATTT CCGGGTGGTC CATCCCCACC ACGCCCTGAA CAAGATCTCC 
AATCGGACAG CAGCCATCAT CTCTTGCCTT CTGTGGGGCA TCACTATTGG CCTGACAGTC 
CACCTCCTGA AGAAGAAGAT GCCGATCCAG AATGGCGGTG CAAATTTGTG CAGCAGCTTC 
AGCATCTGCC ATACCTTCCA GTGGCACGAA GCCATGTTCC TCCTGGAGTT CTTCCTGCCC 
CTGGGCATCA TCCTGTTCTG CTCAGCCAGA ATTATCTGGA GCCTGCGGCA GAGACAAATG 
GACCGGCATG CCAAGATCAA GAGAGCCATC ACCTTCATCA TGGTGGTGGC CATCGTCTTT 
GTCATCTGCT TCCTTCCCAG CGTGGTTGTG CGGATCCGCA TCTTCTGGCT CCTGCACACT 
TCGGGCACGC AGAATTGTGA AGTGTACCGC TCGGTGGACC TGGCGTTCTT TATCACTCTC 
AGCTTCACCT ACATGAACAG CATGCTGGAC CCCGTGGTGT ACTACTTCTC CAGCCCATCC 
TTTCCCAACT TCTTCTCCAC TTTGATCAAC CGCTGCCTCC AGAGGAAGAT GACAGGTGAG 
CCAGATAATA ACCGCAGCAC GAGCGTCGAG CTCACAGGGG ACCCCAACAA AACCAGAGGC 
GCTCCAGAGG CGTTAATGGC CAACTCCGGT GAGCCATGGA GCCCCTCTTA TCTGGGCCCA 
ACCTCTCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCAR2 ( NM_177551.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_808219.1 Homo sapiens hydroxycarboxylic acid receptor 2 (HCAR2), mRNA.

Price & Availability↑

CloneID	OHu17408
Clone ID Related Accession (Same CDS sequence)	NM_177551.4 , NM_177551.3
Accession Version	NM_177551.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	hydroxycarboxylic acid receptor 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL698846.1, BC027965.1, BC056419.1, AL572062.2 and CD364466.1. On Jan 23, 2004 this sequence version replaced NM_177551.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC027965.1 [ECO:0000345] ##Evidence-Data-END##

ATGAATCGGC ACCATCTGCA GGATCACTTT CTGGAAATAG ACAAGAAGAA CTGCTGTGTG 
TTCCGAGATG ACTTCATTGT CAAGGTGTTG CCGCCGGTGT TGGGGCTGGA GTTTATCTTC 
GGGCTTCTGG GCAATGGCCT TGCCCTGTGG ATTTTCTGTT TCCACCTCAA GTCCTGGAAA 
TCCAGCCGGA TTTTCCTGTT CAACCTGGCA GTGGCTGACT TTCTACTGAT CATCTGCCTG 
CCCTTCCTGA TGGACAACTA TGTGAGGCGT TGGGACTGGA AGTTTGGGGA CATCCCTTGC 
CGGCTGATGC TCTTCATGTT GGCTATGAAC CGCCAGGGCA GCATCATCTT CCTCACGGTG 
GTGGCGGTAG ACAGGTATTT CCGGGTGGTC CATCCCCACC ACGCCCTGAA CAAGATCTCC 
AATCGGACAG CAGCCATCAT CTCTTGCCTT CTGTGGGGCA TCACTATTGG CCTGACAGTC 
CACCTCCTGA AGAAGAAGAT GCCGATCCAG AATGGCGGTG CAAATTTGTG CAGCAGCTTC 
AGCATCTGCC ATACCTTCCA GTGGCACGAA GCCATGTTCC TCCTGGAGTT CTTCCTGCCC 
CTGGGCATCA TCCTGTTCTG CTCAGCCAGA ATTATCTGGA GCCTGCGGCA GAGACAAATG 
GACCGGCATG CCAAGATCAA GAGAGCCATC ACCTTCATCA TGGTGGTGGC CATCGTCTTT 
GTCATCTGCT TCCTTCCCAG CGTGGTTGTG CGGATCCGCA TCTTCTGGCT CCTGCACACT 
TCGGGCACGC AGAATTGTGA AGTGTACCGC TCGGTGGACC TGGCGTTCTT TATCACTCTC 
AGCTTCACCT ACATGAACAG CATGCTGGAC CCCGTGGTGT ACTACTTCTC CAGCCCATCC 
TTTCCCAACT TCTTCTCCAC TTTGATCAAC CGCTGCCTCC AGAGGAAGAT GACAGGTGAG 
CCAGATAATA ACCGCAGCAC GAGCGTCGAG CTCACAGGGG ACCCCAACAA AACCAGAGGC 
GCTCCAGAGG CGTTAATGGC CAACTCCGGT GAGCCATGGA GCCCCTCTTA TCTGGGCCCA 
ACCTCTCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808219.1
CDS	75..1166
Translation	MNRHHLQDHFLEIDKKNCCVFRDDFIVKVLPPVLGLEFIFGLLGNGLALWIFCFHLKSWKSSRIFLFNLAVADFLLIICLPFLMDNYVRRWDWKFGDIPCRLMLFMLAMNRQGSIIFLTVVAVDRYFRVVHPHHALNKISNRTAAIISCLLWGITIGLTVHLLKKKMPIQNGGANLCSSFSICHTFQWHEAMFLLEFFLPLGIILFCSARIIWSLRQRQMDRHAKIKRAITFIMVVAIVFVICFLPSVVVRIRIFWLLHTSGTQNCEVYRSVDLAFFITLSFTYMNSMLDPVVYYFSSPSFPNFFSTLINRCLQRKMTGEPDNNRSTSVELTGDPNKTRGAPEALMANSGEPWSPSYLGPTSP

Target ORF information:

RefSeq Version	NM_177551.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hydroxycarboxylic acid receptor 2 (HCAR2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177551.3

ORF Insert Sequence:

ATGAATCGGC ACCATCTGCA GGATCACTTT CTGGAAATAG ACAAGAAGAA CTGCTGTGTG 
TTCCGAGATG ACTTCATTGT CAAGGTGTTG CCGCCGGTGT TGGGGCTGGA GTTTATCTTC 
GGGCTTCTGG GCAATGGCCT TGCCCTGTGG ATTTTCTGTT TCCACCTCAA GTCCTGGAAA 
TCCAGCCGGA TTTTCCTGTT CAACCTGGCA GTGGCTGACT TTCTACTGAT CATCTGCCTG 
CCCTTCCTGA TGGACAACTA TGTGAGGCGT TGGGACTGGA AGTTTGGGGA CATCCCTTGC 
CGGCTGATGC TCTTCATGTT GGCTATGAAC CGCCAGGGCA GCATCATCTT CCTCACGGTG 
GTGGCGGTAG ACAGGTATTT CCGGGTGGTC CATCCCCACC ACGCCCTGAA CAAGATCTCC 
AATCGGACAG CAGCCATCAT CTCTTGCCTT CTGTGGGGCA TCACTATTGG CCTGACAGTC 
CACCTCCTGA AGAAGAAGAT GCCGATCCAG AATGGCGGTG CAAATTTGTG CAGCAGCTTC 
AGCATCTGCC ATACCTTCCA GTGGCACGAA GCCATGTTCC TCCTGGAGTT CTTCCTGCCC 
CTGGGCATCA TCCTGTTCTG CTCAGCCAGA ATTATCTGGA GCCTGCGGCA GAGACAAATG 
GACCGGCATG CCAAGATCAA GAGAGCCATC ACCTTCATCA TGGTGGTGGC CATCGTCTTT 
GTCATCTGCT TCCTTCCCAG CGTGGTTGTG CGGATCCGCA TCTTCTGGCT CCTGCACACT 
TCGGGCACGC AGAATTGTGA AGTGTACCGC TCGGTGGACC TGGCGTTCTT TATCACTCTC 
AGCTTCACCT ACATGAACAG CATGCTGGAC CCCGTGGTGT ACTACTTCTC CAGCCCATCC 
TTTCCCAACT TCTTCTCCAC TTTGATCAAC CGCTGCCTCC AGAGGAAGAT GACAGGTGAG 
CCAGATAATA ACCGCAGCAC GAGCGTCGAG CTCACAGGGG ACCCCAACAA AACCAGAGGC 
GCTCCAGAGG CGTTAATGGC CAACTCCGGT GAGCCATGGA GCCCCTCTTA TCTGGGCCCA 
ACCTCTCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nicotinic Acid Receptor GPR109A Exerts Anti-Inflammatory Effects Through Inhibiting the Akt/mTOR Signaling Pathway in MIN6 Pancreatic ? cells.
Annals of clinical and laboratory science47(6)729-737(2017 Nov)
Li Z,Li X,Lin S,Chen Y,Ma S,Fu Y,Wei C,Xu W

Niacin receptor GPR109A inhibits insulin secretion and is down-regulated in type 2 diabetic islet beta-cells.
General and comparative endocrinology23798-108(2016 Oct)
Wang N,Guo DY,Tian X,Lin HP,Li YP,Chen SJ,Fu YC,Xu WC,Wei CJ

The role of the C-terminus of the human hydroxycarboxylic acid receptors 2 and 3 in G protein activation using G?-engineered yeast cells.
European journal of pharmacology77070-7(2016 Jan)
Liu R,van Veldhoven JP,IJzerman AP

The atypical N-glycosylation motif, Asn-Cys-Cys, in human GPR109A is required for normal cell surface expression and intracellular signaling.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology29(6)2412-22(2015 Jun)
Yasuda D,Imura Y,Ishii S,Shimizu T,Nakamura M

Niacin receptor activation improves human microvascular endothelial cell angiogenic function during lipotoxicity.
Atherosclerosis237(2)696-704(2014 Dec)
Hughes-Large JM,Pang DK,Robson DL,Chan P,Toma J,Borradaile NM

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HCAR2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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