HUS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HUS1
Entrez Gene ID	3364
Full Name	HUS1 checkpoint clamp component
Synonyms	hHUS1
General protein information	Preferred Names HUS1 checkpoint clamp component Names checkpoint protein HUS1 HUS1 checkpoint homolog hus1+-like protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p12.3
Summary	The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011].
Disorder MIM:	603760

mRNA and Protein(s)

mRNA	Protein	Name
XM_017012115.1	XP_016867604.1	checkpoint protein HUS1 isoform X1
NM_001363683.1	NP_001350612.1	checkpoint protein HUS1 isoform 2
NM_004507.4	NP_004498.1	checkpoint protein HUS1 isoform 1
NM_001363683.2	NP_001350612.1	checkpoint protein HUS1 isoform 2
NM_004507.3	NP_004498.1	checkpoint protein HUS1

Pathways from BioSystems

Homologies

	HUS1	XP_001088000.1
Danio rerio (zebrafish)	zgc:162895	NP_001082965.1
Magnaporthe oryzae (rice blast fungus)	MGG_12138	XP_003719779.1
Neurospora crassa	NCU11262	XP_001728351.1
Rattus norvegicus (Norway rat)	Hus1	NP_001102562.1
Caenorhabditis elegans (roundworm)	hus-1	NP_491203.1
Schizosaccharomyces pombe (fission yeast)	hus1	NP_594739.1
Homo sapiens (human)	HUS1	NP_004498.1
Canis lupus familiaris (dog)	HUS1	XP_848291.3
Mus musculus (house mouse)	Hus1	NP_032342.1
Drosophila melanogaster (fruit fly)	Hus1-like	NP_001262267.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011361	XP_317958.4
Xenopus tropicalis (tropical clawed frog)	hus1	NP_001006827.1
Pan troglodytes (chimpanzee)	HUS1	XP_003951051.1
Gallus gallus (chicken)	HUS1	XP_426029.3

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

The Roles of p21(Waf1/CIP1) and Hus1 in Generation and Transmission of Damage Signals Stimulated by Low-Dose Alpha-Particle Irradiation.
Zhao Y, Ma X, Wang J, Chen S, Yuan H, Xu A, Hang H, Wu L
Radiation research184(6)578-85(2015 Dec)

Distinct functional consequences of MUTYH variants associated with colorectal cancer: Damaged DNA affinity, glycosylase activity and interaction with PCNA and Hus1.
Brinkmeyer MK, David SS
DNA repair3439-51(2015 Oct)

Intramolecular Binding of the Rad9 C Terminus in the Checkpoint Clamp Rad9-Hus1-Rad1 Is Closely Linked with Its DNA Binding.
Takeishi Y, Iwaya-Omi R, Ohashi E, Tsurimoto T
The Journal of biological chemistry290(32)19923-32(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that p21(Waf1/CIP1) and Hus1 play crucial roles in the generation and transmission of bystander damage signals after low-dose alpha-particle irradiation.
Title: The Roles of p21(Waf1/CIP1) and Hus1 in Generation and Transmission of Damage Signals Stimulated by Low-Dose Alpha-Particle Irradiation.

adenine glycosylase activity, mismatch recognition properties and interaction with protein partners of MUTYH and 5 MAP variants were examined; P502L and R520Q had reduced affinity for PCNA; only Q324H was found to have reduced affinity for Hus1
Title: Distinct functional consequences of MUTYH variants associated with colorectal cancer: Damaged DNA affinity, glycosylase activity and interaction with PCNA and Hus1.

Intramolecular binding of the rad9 C-terminus in the checkpoint clamp Rad9-Hus1-Rad1 is closely linked with its DNA binding.
Title: Intramolecular Binding of the Rad9 C Terminus in the Checkpoint Clamp Rad9-Hus1-Rad1 Is Closely Linked with Its DNA Binding.

these pockets were not required for 9-1-1 chromatin localization or ATR-mediated CHK1 activation but were necessary for interactions between HUS1 and its binding partner MYH
Title: Genome Protection by the 9-1-1 Complex Subunit HUS1 Requires Clamp Formation, DNA Contacts, and ATR Signaling-independent Effector Functions.

Expression of cell cycle regulatory factors hus1, gadd45a, rb1, cdkn2a and mre11a correlates with expression of clock gene per2 in human colorectal carcinoma tissue.
Title: Expression of cell cycle regulatory factors hus1, gadd45a, rb1, cdkn2a and mre11a correlates with expression of clock gene per2 in human colorectal carcinoma tissue.

The following HUS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HUS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01236	NM_004507.4 Latest version!	Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu97208	NM_001363683.2 Latest version!	Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu97208	XM_017012115.1 Latest version!	Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu97208	NM_001363683.1	Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu01236	NM_004507.3	Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HUS1 ( NM_004507.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004498.1 Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01236
Clone ID Related Accession (Same CDS sequence)	NM_004507.4 , NM_004507.3
Accession Version	NM_004507.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	checkpoint protein HUS1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG286955.1, BC007013.1, AC069282.6 and CA438762.1. On Nov 23, 2018 this sequence version replaced NM_004507.3. Transcript Variant: This variant (1) represents the longer transcript and encodes a protein of 280 aa. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.198252.1, SRR1803616.145768.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000258774.10/ ENSP00000258774.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAGTTTC GGGCCAAGAT CGTGGACGGG GCCTGTCTGA ACCACTTCAC ACGAATCAGT 
AACATGATAG CCAAGCTTGC CAAAACCTGC ACCCTCCGCA TCAGCCCTGA TAAGCTTAAC 
TTCATCCTTT GTGACAAGCT GGCTAATGGA GGAGTGAGCA TGTGGTGTGA GCTGGAACAG 
GAGAACTTCT TCAACGAATT TCAAATGGAG GGTGTCTCTG CAGAAAACAA TGAGATTTAT 
TTAGAGCTAA CATCGGAAAA CTTATCTCGA GCCTTGAAGA CTGCCCAGAA TGCCAGGGCT 
TTGAAAATCA AACTGACTAA TAAACACTTT CCCTGCCTCA CGGTCTCCGT GGAGCTGTTA 
TCTATGTCAA GCAGTAGCCG CATTGTGACC CATGACATCC CCATAAAGGT GATTCCTAGG 
AAATTGTGGA AGGACTTACA AGAACCGGTG GTCCCAGATC CTGATGTTAG TATTTATTTA 
CCAGTCTTGA AGACTATGAA GAGTGTTGTG GAAAAAATGA AAAACATCAG CAATCACCTT 
GTTATTGAAG CAAACCTAGA TGGAGAATTG AATTTGAAAA TAGAAACTGA ATTAGTATGT 
GTTACAACTC ATTTTAAAGA TCTTGGAAAT CCTCCATTAG CCTCTGAAAG CACCCATGAG 
GACAGAAACG TGGAACACAT GGCTGAAGTG CACATAGATA TTAGGAAGCT CCTACAGTTT 
CTTGCTGGAC AACAAGTAAA TCCCACAAAG GCCTTATGCA ATATTGTGAA TAACAAGATG 
GTGCATTTTG ATCTGCTTCA TGAAGACGTG TCCCTTCAGT ATTTCATCCC TGCGCTGTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004498.1
CDS	97..939
Translation	MKFRAKIVDGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS

Target ORF information:

RefSeq Version	NM_004507.4
Organism	Homo sapiens(human)
Definition	Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004507.4

ORF Insert Sequence:

ATGAAGTTTC GGGCCAAGAT CGTGGACGGG GCCTGTCTGA ACCACTTCAC ACGAATCAGT 
AACATGATAG CCAAGCTTGC CAAAACCTGC ACCCTCCGCA TCAGCCCTGA TAAGCTTAAC 
TTCATCCTTT GTGACAAGCT GGCTAATGGA GGAGTGAGCA TGTGGTGTGA GCTGGAACAG 
GAGAACTTCT TCAACGAATT TCAAATGGAG GGTGTCTCTG CAGAAAACAA TGAGATTTAT 
TTAGAGCTAA CATCGGAAAA CTTATCTCGA GCCTTGAAGA CTGCCCAGAA TGCCAGGGCT 
TTGAAAATCA AACTGACTAA TAAACACTTT CCCTGCCTCA CGGTCTCCGT GGAGCTGTTA 
TCTATGTCAA GCAGTAGCCG CATTGTGACC CATGACATCC CCATAAAGGT GATTCCTAGG 
AAATTGTGGA AGGACTTACA AGAACCGGTG GTCCCAGATC CTGATGTTAG TATTTATTTA 
CCAGTCTTGA AGACTATGAA GAGTGTTGTG GAAAAAATGA AAAACATCAG CAATCACCTT 
GTTATTGAAG CAAACCTAGA TGGAGAATTG AATTTGAAAA TAGAAACTGA ATTAGTATGT 
GTTACAACTC ATTTTAAAGA TCTTGGAAAT CCTCCATTAG CCTCTGAAAG CACCCATGAG 
GACAGAAACG TGGAACACAT GGCTGAAGTG CACATAGATA TTAGGAAGCT CCTACAGTTT 
CTTGCTGGAC AACAAGTAAA TCCCACAAAG GCCTTATGCA ATATTGTGAA TAACAAGATG 
GTGCATTTTG ATCTGCTTCA TGAAGACGTG TCCCTTCAGT ATTTCATCCC TGCGCTGTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUS1 ( NM_004507.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004498.1 Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01236
Clone ID Related Accession (Same CDS sequence)	NM_004507.4 , NM_004507.3
Accession Version	NM_004507.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	checkpoint protein HUS1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG286955.1, BC007013.1, AC069282.6 and CA438762.1. On Mar 1, 2011 this sequence version replaced NM_004507.2. Transcript Variant: This variant (1) represents the longer transcript and encodes a protein of 280 aa. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.198252.1, SRR1803616.145768.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGTTTC GGGCCAAGAT CGTGGACGGG GCCTGTCTGA ACCACTTCAC ACGAATCAGT 
AACATGATAG CCAAGCTTGC CAAAACCTGC ACCCTCCGCA TCAGCCCTGA TAAGCTTAAC 
TTCATCCTTT GTGACAAGCT GGCTAATGGA GGAGTGAGCA TGTGGTGTGA GCTGGAACAG 
GAGAACTTCT TCAACGAATT TCAAATGGAG GGTGTCTCTG CAGAAAACAA TGAGATTTAT 
TTAGAGCTAA CATCGGAAAA CTTATCTCGA GCCTTGAAGA CTGCCCAGAA TGCCAGGGCT 
TTGAAAATCA AACTGACTAA TAAACACTTT CCCTGCCTCA CGGTCTCCGT GGAGCTGTTA 
TCTATGTCAA GCAGTAGCCG CATTGTGACC CATGACATCC CCATAAAGGT GATTCCTAGG 
AAATTGTGGA AGGACTTACA AGAACCGGTG GTCCCAGATC CTGATGTTAG TATTTATTTA 
CCAGTCTTGA AGACTATGAA GAGTGTTGTG GAAAAAATGA AAAACATCAG CAATCACCTT 
GTTATTGAAG CAAACCTAGA TGGAGAATTG AATTTGAAAA TAGAAACTGA ATTAGTATGT 
GTTACAACTC ATTTTAAAGA TCTTGGAAAT CCTCCATTAG CCTCTGAAAG CACCCATGAG 
GACAGAAACG TGGAACACAT GGCTGAAGTG CACATAGATA TTAGGAAGCT CCTACAGTTT 
CTTGCTGGAC AACAAGTAAA TCCCACAAAG GCCTTATGCA ATATTGTGAA TAACAAGATG 
GTGCATTTTG ATCTGCTTCA TGAAGACGTG TCCCTTCAGT ATTTCATCCC TGCGCTGTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004498.1
CDS	107..949
Translation	MKFRAKIVDGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS

Target ORF information:

RefSeq Version	NM_004507.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004507.3

ORF Insert Sequence:

ATGAAGTTTC GGGCCAAGAT CGTGGACGGG GCCTGTCTGA ACCACTTCAC ACGAATCAGT 
AACATGATAG CCAAGCTTGC CAAAACCTGC ACCCTCCGCA TCAGCCCTGA TAAGCTTAAC 
TTCATCCTTT GTGACAAGCT GGCTAATGGA GGAGTGAGCA TGTGGTGTGA GCTGGAACAG 
GAGAACTTCT TCAACGAATT TCAAATGGAG GGTGTCTCTG CAGAAAACAA TGAGATTTAT 
TTAGAGCTAA CATCGGAAAA CTTATCTCGA GCCTTGAAGA CTGCCCAGAA TGCCAGGGCT 
TTGAAAATCA AACTGACTAA TAAACACTTT CCCTGCCTCA CGGTCTCCGT GGAGCTGTTA 
TCTATGTCAA GCAGTAGCCG CATTGTGACC CATGACATCC CCATAAAGGT GATTCCTAGG 
AAATTGTGGA AGGACTTACA AGAACCGGTG GTCCCAGATC CTGATGTTAG TATTTATTTA 
CCAGTCTTGA AGACTATGAA GAGTGTTGTG GAAAAAATGA AAAACATCAG CAATCACCTT 
GTTATTGAAG CAAACCTAGA TGGAGAATTG AATTTGAAAA TAGAAACTGA ATTAGTATGT 
GTTACAACTC ATTTTAAAGA TCTTGGAAAT CCTCCATTAG CCTCTGAAAG CACCCATGAG 
GACAGAAACG TGGAACACAT GGCTGAAGTG CACATAGATA TTAGGAAGCT CCTACAGTTT 
CTTGCTGGAC AACAAGTAAA TCCCACAAAG GCCTTATGCA ATATTGTGAA TAACAAGATG 
GTGCATTTTG ATCTGCTTCA TGAAGACGTG TCCCTTCAGT ATTTCATCCC TGCGCTGTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUS1 ( NM_001363683.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350612.1 Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu97208
Clone ID Related Accession (Same CDS sequence)	XM_017012115.1 , NM_001363683.1 , NM_001363683.2
Accession Version	NM_001363683.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-01
Organism	Homo sapiens(human)
Product	checkpoint protein HUS1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC069282.6. On May 30, 2018 this sequence version replaced XM_017012115.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.135254.1, SRR5189667.254081.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGATAGCCA AGCTTGCCAA AACCTGCACC CTCCGCATCA GCCCTGATAA GCTTAACTTC 
ATCCTTTGTG ACAAGCTGGC TAATGGAGGA GTGAGCATGT GGTGTGAGCT GGAACAGGAG 
AACTTCTTCA ACGAATTTCA AATGGAGGGT GTCTCTGCAG AAAACAATGA GATTTATTTA 
GAGCTAACAT CGGAAAACTT ATCTCGAGCC TTGAAGACTG CCCAGAATGC CAGGGCTTTG 
AAAATCAAAC TGACTAATAA ACACTTTCCC TGCCTCACGG TCTCCGTGGA GCTGTTATCT 
ATGTCAAGCA GTAGCCGCAT TGTGACCCAT GACATCCCCA TAAAGGTGAT TCCTAGGAAA 
TTGTGGAAGG ACTTACAAGA ACCGGTGGTC CCAGATCCTG ATGTTAGTAT TTATTTACCA 
GTCTTGAAGA CTATGAAGAG TGTTGTGGAA AAAATGAAAA ACATCAGCAA TCACCTTGTT 
ATTGAAGCAA ACCTAGATGG AGAATTGAAT TTGAAAATAG AAACTGAATT AGTATGTGTT 
ACAACTCATT TTAAAGATCT TGGAAATCCT CCATTAGCCT CTGAAAGCAC CCATGAGGAC 
AGAAACGTGG AACACATGGC TGAAGTGCAC ATAGATATTA GGAAGCTCCT ACAGTTTCTT 
GCTGGACAAC AAGTAAATCC CACAAAGGCC TTATGCAATA TTGTGAATAA CAAGATGGTG 
CATTTTGATC TGCTTCATGA AGACGTGTCC CTTCAGTATT TCATCCCTGC GCTGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350612.1
CDS	132..911
Translation	MIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS

Target ORF information:

RefSeq Version	NM_001363683.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363683.1

ORF Insert Sequence:

ATGATAGCCA AGCTTGCCAA AACCTGCACC CTCCGCATCA GCCCTGATAA GCTTAACTTC 
ATCCTTTGTG ACAAGCTGGC TAATGGAGGA GTGAGCATGT GGTGTGAGCT GGAACAGGAG 
AACTTCTTCA ACGAATTTCA AATGGAGGGT GTCTCTGCAG AAAACAATGA GATTTATTTA 
GAGCTAACAT CGGAAAACTT ATCTCGAGCC TTGAAGACTG CCCAGAATGC CAGGGCTTTG 
AAAATCAAAC TGACTAATAA ACACTTTCCC TGCCTCACGG TCTCCGTGGA GCTGTTATCT 
ATGTCAAGCA GTAGCCGCAT TGTGACCCAT GACATCCCCA TAAAGGTGAT TCCTAGGAAA 
TTGTGGAAGG ACTTACAAGA ACCGGTGGTC CCAGATCCTG ATGTTAGTAT TTATTTACCA 
GTCTTGAAGA CTATGAAGAG TGTTGTGGAA AAAATGAAAA ACATCAGCAA TCACCTTGTT 
ATTGAAGCAA ACCTAGATGG AGAATTGAAT TTGAAAATAG AAACTGAATT AGTATGTGTT 
ACAACTCATT TTAAAGATCT TGGAAATCCT CCATTAGCCT CTGAAAGCAC CCATGAGGAC 
AGAAACGTGG AACACATGGC TGAAGTGCAC ATAGATATTA GGAAGCTCCT ACAGTTTCTT 
GCTGGACAAC AAGTAAATCC CACAAAGGCC TTATGCAATA TTGTGAATAA CAAGATGGTG 
CATTTTGATC TGCTTCATGA AGACGTGTCC CTTCAGTATT TCATCCCTGC GCTGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUS1 ( NM_001363683.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350612.1 Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu97208
Clone ID Related Accession (Same CDS sequence)	XM_017012115.1 , NM_001363683.1 , NM_001363683.2
Accession Version	NM_001363683.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	checkpoint protein HUS1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC069282.6. On May 31, 2019 this sequence version replaced NM_001363683.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.135254.1, SRR1163658.414474.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGATAGCCA AGCTTGCCAA AACCTGCACC CTCCGCATCA GCCCTGATAA GCTTAACTTC 
ATCCTTTGTG ACAAGCTGGC TAATGGAGGA GTGAGCATGT GGTGTGAGCT GGAACAGGAG 
AACTTCTTCA ACGAATTTCA AATGGAGGGT GTCTCTGCAG AAAACAATGA GATTTATTTA 
GAGCTAACAT CGGAAAACTT ATCTCGAGCC TTGAAGACTG CCCAGAATGC CAGGGCTTTG 
AAAATCAAAC TGACTAATAA ACACTTTCCC TGCCTCACGG TCTCCGTGGA GCTGTTATCT 
ATGTCAAGCA GTAGCCGCAT TGTGACCCAT GACATCCCCA TAAAGGTGAT TCCTAGGAAA 
TTGTGGAAGG ACTTACAAGA ACCGGTGGTC CCAGATCCTG ATGTTAGTAT TTATTTACCA 
GTCTTGAAGA CTATGAAGAG TGTTGTGGAA AAAATGAAAA ACATCAGCAA TCACCTTGTT 
ATTGAAGCAA ACCTAGATGG AGAATTGAAT TTGAAAATAG AAACTGAATT AGTATGTGTT 
ACAACTCATT TTAAAGATCT TGGAAATCCT CCATTAGCCT CTGAAAGCAC CCATGAGGAC 
AGAAACGTGG AACACATGGC TGAAGTGCAC ATAGATATTA GGAAGCTCCT ACAGTTTCTT 
GCTGGACAAC AAGTAAATCC CACAAAGGCC TTATGCAATA TTGTGAATAA CAAGATGGTG 
CATTTTGATC TGCTTCATGA AGACGTGTCC CTTCAGTATT TCATCCCTGC GCTGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350612.1
CDS	218..997
Translation	MIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS

Target ORF information:

RefSeq Version	NM_001363683.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363683.2

ORF Insert Sequence:

ATGATAGCCA AGCTTGCCAA AACCTGCACC CTCCGCATCA GCCCTGATAA GCTTAACTTC 
ATCCTTTGTG ACAAGCTGGC TAATGGAGGA GTGAGCATGT GGTGTGAGCT GGAACAGGAG 
AACTTCTTCA ACGAATTTCA AATGGAGGGT GTCTCTGCAG AAAACAATGA GATTTATTTA 
GAGCTAACAT CGGAAAACTT ATCTCGAGCC TTGAAGACTG CCCAGAATGC CAGGGCTTTG 
AAAATCAAAC TGACTAATAA ACACTTTCCC TGCCTCACGG TCTCCGTGGA GCTGTTATCT 
ATGTCAAGCA GTAGCCGCAT TGTGACCCAT GACATCCCCA TAAAGGTGAT TCCTAGGAAA 
TTGTGGAAGG ACTTACAAGA ACCGGTGGTC CCAGATCCTG ATGTTAGTAT TTATTTACCA 
GTCTTGAAGA CTATGAAGAG TGTTGTGGAA AAAATGAAAA ACATCAGCAA TCACCTTGTT 
ATTGAAGCAA ACCTAGATGG AGAATTGAAT TTGAAAATAG AAACTGAATT AGTATGTGTT 
ACAACTCATT TTAAAGATCT TGGAAATCCT CCATTAGCCT CTGAAAGCAC CCATGAGGAC 
AGAAACGTGG AACACATGGC TGAAGTGCAC ATAGATATTA GGAAGCTCCT ACAGTTTCTT 
GCTGGACAAC AAGTAAATCC CACAAAGGCC TTATGCAATA TTGTGAATAA CAAGATGGTG 
CATTTTGATC TGCTTCATGA AGACGTGTCC CTTCAGTATT TCATCCCTGC GCTGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HUS1 ( XM_017012115.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867604.1 Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript variant X3, mRNA.

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CloneID	OHu97208
Clone ID Related Accession (Same CDS sequence)	XM_017012115.1 , NM_001363683.1 , NM_001363683.2
Accession Version	XM_017012115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	checkpoint protein HUS1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGCCA AGCTTGCCAA AACCTGCACC CTCCGCATCA GCCCTGATAA GCTTAACTTC 
ATCCTTTGTG ACAAGCTGGC TAATGGAGGA GTGAGCATGT GGTGTGAGCT GGAACAGGAG 
AACTTCTTCA ACGAATTTCA AATGGAGGGT GTCTCTGCAG AAAACAATGA GATTTATTTA 
GAGCTAACAT CGGAAAACTT ATCTCGAGCC TTGAAGACTG CCCAGAATGC CAGGGCTTTG 
AAAATCAAAC TGACTAATAA ACACTTTCCC TGCCTCACGG TCTCCGTGGA GCTGTTATCT 
ATGTCAAGCA GTAGCCGCAT TGTGACCCAT GACATCCCCA TAAAGGTGAT TCCTAGGAAA 
TTGTGGAAGG ACTTACAAGA ACCGGTGGTC CCAGATCCTG ATGTTAGTAT TTATTTACCA 
GTCTTGAAGA CTATGAAGAG TGTTGTGGAA AAAATGAAAA ACATCAGCAA TCACCTTGTT 
ATTGAAGCAA ACCTAGATGG AGAATTGAAT TTGAAAATAG AAACTGAATT AGTATGTGTT 
ACAACTCATT TTAAAGATCT TGGAAATCCT CCATTAGCCT CTGAAAGCAC CCATGAGGAC 
AGAAACGTGG AACACATGGC TGAAGTGCAC ATAGATATTA GGAAGCTCCT ACAGTTTCTT 
GCTGGACAAC AAGTAAATCC CACAAAGGCC TTATGCAATA TTGTGAATAA CAAGATGGTG 
CATTTTGATC TGCTTCATGA AGACGTGTCC CTTCAGTATT TCATCCCTGC GCTGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867604.1
CDS	168..947
Translation	MIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS

Target ORF information:

RefSeq Version	XM_017012115.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012115.1

ORF Insert Sequence:

ATGATAGCCA AGCTTGCCAA AACCTGCACC CTCCGCATCA GCCCTGATAA GCTTAACTTC 
ATCCTTTGTG ACAAGCTGGC TAATGGAGGA GTGAGCATGT GGTGTGAGCT GGAACAGGAG 
AACTTCTTCA ACGAATTTCA AATGGAGGGT GTCTCTGCAG AAAACAATGA GATTTATTTA 
GAGCTAACAT CGGAAAACTT ATCTCGAGCC TTGAAGACTG CCCAGAATGC CAGGGCTTTG 
AAAATCAAAC TGACTAATAA ACACTTTCCC TGCCTCACGG TCTCCGTGGA GCTGTTATCT 
ATGTCAAGCA GTAGCCGCAT TGTGACCCAT GACATCCCCA TAAAGGTGAT TCCTAGGAAA 
TTGTGGAAGG ACTTACAAGA ACCGGTGGTC CCAGATCCTG ATGTTAGTAT TTATTTACCA 
GTCTTGAAGA CTATGAAGAG TGTTGTGGAA AAAATGAAAA ACATCAGCAA TCACCTTGTT 
ATTGAAGCAA ACCTAGATGG AGAATTGAAT TTGAAAATAG AAACTGAATT AGTATGTGTT 
ACAACTCATT TTAAAGATCT TGGAAATCCT CCATTAGCCT CTGAAAGCAC CCATGAGGAC 
AGAAACGTGG AACACATGGC TGAAGTGCAC ATAGATATTA GGAAGCTCCT ACAGTTTCTT 
GCTGGACAAC AAGTAAATCC CACAAAGGCC TTATGCAATA TTGTGAATAA CAAGATGGTG 
CATTTTGATC TGCTTCATGA AGACGTGTCC CTTCAGTATT TCATCCCTGC GCTGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

The Roles of p21(Waf1/CIP1) and Hus1 in Generation and Transmission of Damage Signals Stimulated by Low-Dose Alpha-Particle Irradiation.
Radiation research184(6)578-85(2015 Dec)
Zhao Y,Ma X,Wang J,Chen S,Yuan H,Xu A,Hang H,Wu L

Distinct functional consequences of MUTYH variants associated with colorectal cancer: Damaged DNA affinity, glycosylase activity and interaction with PCNA and Hus1.
DNA repair3439-51(2015 Oct)
Brinkmeyer MK,David SS

Intramolecular Binding of the Rad9 C Terminus in the Checkpoint Clamp Rad9-Hus1-Rad1 Is Closely Linked with Its DNA Binding.
The Journal of biological chemistry290(32)19923-32(2015 Aug)
Takeishi Y,Iwaya-Omi R,Ohashi E,Tsurimoto T

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HUS1 cDNA ORF clone, Homo sapiens(human)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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