HSPB1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSPB1
Entrez Gene ID	3315
Full Name	heat shock protein family B (small) member 1
Synonyms	CMT2F,HEL-S-102,HMN2B,HS.76067,HSP27,HSP28,Hsp25,SRP27
General protein information	Preferred Names heat shock protein family B (small) member 1 Names heat shock protein beta-1 28 kDa heat shock protein epididymis secretory protein Li 102 estrogen-regulated 24 kDa protein heat shock 27 kDa protein heat shock 27kD protein 1 heat shock 27kDa protein 1 stress-responsive protein 27
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q11.23
Summary	This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017].
Disorder MIM:	602195
Disorder Html:	Neuropathy, distal hereditary motor, type IIB, 608634 (3); Charcot-Marie-Tooth disease, axonal, type 2F, 606595 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001540.4	NP_001531.1	heat shock protein beta-1
NM_001540.3	NP_001531.1	heat shock protein beta-1
NM_001540.5	NP_001531.1	heat shock protein beta-1

Pathways from BioSystems

Homologies

	HSPB1	XP_001109274.1
Canis lupus familiaris (dog)	HSPB1	NP_001003295.1
Danio rerio (zebrafish)	hspb1	NP_001008615.2
Mus musculus (house mouse)	Hspb1	NP_038588.2
Homo sapiens (human)	HSPB1	NP_001531.1
Bos taurus (cattle)	HSPB1	NP_001020740.1
Gallus gallus (chicken)	HSPB1	NP_990621.1
Pan troglodytes (chimpanzee)	HSPB1	XP_003951106.1
Rattus norvegicus (Norway rat)	Hspb1	NP_114176.3
Xenopus tropicalis (tropical clawed frog)	hspb1	NP_001072817.1

Gene Ontology
Bibliography

Related articles in PubMed

The expression and correlation of Hsp 70 and Hsp 27 in serous middle ear effusion fluids of pediatric patients-a preliminary study.
Min HJ, Choe JW, Chang MY, Kim KS, Lee SY, Mun SK
International journal of pediatric otorhinolaryngology101145-149(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hsp 70 and Hsp 27 were expressed in middle ear effusions
Title: The expression and correlation of Hsp 70 and Hsp 27 in serous middle ear effusion fluids of pediatric patients-a preliminary study.

Data suggest that HSPB6 forms hetero-oligomers with HSPB1 under the following rules: (1) highly conserved motif RLFDQXFG is necessary for subunit exchange among oligomers, (2) a site about 20 residues downstream of this motif determines size of resultant hetero-oligomers, and (3) a region in the N-terminal domain that is unique to HSPB6 dictates preferential formation of heterodimers. (HSP = heat shock protein)
Title: Specific sequences in the N-terminal domain of human small heat-shock protein HSPB6 dictate preferential hetero-oligomerization with the orthologue HSPB1.

Bradykinin stimulates myofibroblast migration through protein kinase D-mediated activation of COX-2 and Hsp27.
Title: Bradykinin stimulates protein kinase D-mediated colonic myofibroblast migration via cyclooxygenase-2 and heat shock protein 27.

Addition of the Hsp27 inhibitor KRIBB3 (5-(5-ethyl-2-hydroxy-4-methoxyphenyl)-4-(4-methoxyphenyl)-isoxazol) to Jurkat cells resulted in glutathione redox imbalance (increased GSSG and increased glutathione reductase activity), a decrease in Hsp70 concentrations, and also increased cell apoptosis
Title: [The role of heat shock proteins 27 and 70 in redox-dependent regulation of apoptosis in Jurkat tumor cells].

This work examines the molecular mechanism by which two canonical sHsps, alphaB-crystallin (alphaB-c) and Hsp27, interact with aggregation-prone alpha-syn to prevent its aggregation in vitro Both sHsps are very effective inhibitors of alpha-syn aggregation
Title: Small Heat-shock Proteins Prevent ?-Synuclein Aggregation via Transient Interactions and Their Efficacy Is Affected by the Rate of Aggregation.

The following HSPB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSPB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26288	NM_001540.5 Latest version!	Homo sapiens heat shock protein family B (small) member 1 (HSPB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu26288	NM_001540.3	Homo sapiens heat shock protein family B (small) member 1 (HSPB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu26288	NM_001540.4	Homo sapiens heat shock protein family B (small) member 1 (HSPB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSPB1 ( NM_001540.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001531.1 Homo sapiens heat shock protein family B (small) member 1 (HSPB1), mRNA.

Price & Availability↑

CloneID	OHu26288
Clone ID Related Accession (Same CDS sequence)	NM_001540.4 , NM_001540.3 , NM_001540.5
Accession Version	NM_001540.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	heat shock protein beta-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP231480.1, BC012768.2 and AW273562.1. On Sep 13, 2017 this sequence version replaced NM_001540.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.334439.1, BM915551.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGAGC GCCGCGTCCC CTTCTCGCTC CTGCGGGGCC CCAGCTGGGA CCCCTTCCGC 
GACTGGTACC CGCATAGCCG CCTCTTCGAC CAGGCCTTCG GGCTGCCCCG GCTGCCGGAG 
GAGTGGTCGC AGTGGTTAGG CGGCAGCAGC TGGCCAGGCT ACGTGCGCCC CCTGCCCCCC 
GCCGCCATCG AGAGCCCCGC AGTGGCCGCG CCCGCCTACA GCCGCGCGCT CAGCCGGCAA 
CTCAGCAGCG GGGTCTCGGA GATCCGGCAC ACTGCGGACC GCTGGCGCGT GTCCCTGGAT 
GTCAACCACT TCGCCCCGGA CGAGCTGACG GTCAAGACCA AGGATGGCGT GGTGGAGATC 
ACCGGCAAGC ACGAGGAGCG GCAGGACGAG CATGGCTACA TCTCCCGGTG CTTCACGCGG 
AAATACACGC TGCCCCCCGG TGTGGACCCC ACCCAAGTTT CCTCCTCCCT GTCCCCTGAG 
GGCACACTGA CCGTGGAGGC CCCCATGCCC AAGCTAGCCA CGCAGTCCAA CGAGATCACC 
ATCCCAGTCA CCTTCGAGTC GCGGGCCCAG CTTGGGGGCC CAGAAGCTGC AAAATCCGAT 
GAGACTGCCG CCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001531.1
CDS	156..773
Translation	MTERRVPFSLLRGPSWDPFRDWYPHSRLFDQAFGLPRLPEEWSQWLGGSSWPGYVRPLPPAAIESPAVAAPAYSRALSRQLSSGVSEIRHTADRWRVSLDVNHFAPDELTVKTKDGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDPTQVSSSLSPEGTLTVEAPMPKLATQSNEITIPVTFESRAQLGGPEAAKSDETAAK

Target ORF information:

RefSeq Version	NM_001540.4
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock protein family B (small) member 1 (HSPB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001540.4

ORF Insert Sequence:

ATGACCGAGC GCCGCGTCCC CTTCTCGCTC CTGCGGGGCC CCAGCTGGGA CCCCTTCCGC 
GACTGGTACC CGCATAGCCG CCTCTTCGAC CAGGCCTTCG GGCTGCCCCG GCTGCCGGAG 
GAGTGGTCGC AGTGGTTAGG CGGCAGCAGC TGGCCAGGCT ACGTGCGCCC CCTGCCCCCC 
GCCGCCATCG AGAGCCCCGC AGTGGCCGCG CCCGCCTACA GCCGCGCGCT CAGCCGGCAA 
CTCAGCAGCG GGGTCTCGGA GATCCGGCAC ACTGCGGACC GCTGGCGCGT GTCCCTGGAT 
GTCAACCACT TCGCCCCGGA CGAGCTGACG GTCAAGACCA AGGATGGCGT GGTGGAGATC 
ACCGGCAAGC ACGAGGAGCG GCAGGACGAG CATGGCTACA TCTCCCGGTG CTTCACGCGG 
AAATACACGC TGCCCCCCGG TGTGGACCCC ACCCAAGTTT CCTCCTCCCT GTCCCCTGAG 
GGCACACTGA CCGTGGAGGC CCCCATGCCC AAGCTAGCCA CGCAGTCCAA CGAGATCACC 
ATCCCAGTCA CCTTCGAGTC GCGGGCCCAG CTTGGGGGCC CAGAAGCTGC AAAATCCGAT 
GAGACTGCCG CCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPB1 ( NM_001540.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001531.1 Homo sapiens heat shock protein family B (small) member 1 (HSPB1), mRNA.

Price & Availability↑

CloneID	OHu26288
Clone ID Related Accession (Same CDS sequence)	NM_001540.4 , NM_001540.3 , NM_001540.5
Accession Version	NM_001540.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-04
Organism	Homo sapiens(Human)
Product	heat shock protein beta-1
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP231480.1 and BC012768.2. This sequence is a reference standard in the RefSeqGene project. On Oct 25, 2008 this sequence version replaced NM_001540.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.334439.1, BM915551.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGAGC GCCGCGTCCC CTTCTCGCTC CTGCGGGGCC CCAGCTGGGA CCCCTTCCGC 
GACTGGTACC CGCATAGCCG CCTCTTCGAC CAGGCCTTCG GGCTGCCCCG GCTGCCGGAG 
GAGTGGTCGC AGTGGTTAGG CGGCAGCAGC TGGCCAGGCT ACGTGCGCCC CCTGCCCCCC 
GCCGCCATCG AGAGCCCCGC AGTGGCCGCG CCCGCCTACA GCCGCGCGCT CAGCCGGCAA 
CTCAGCAGCG GGGTCTCGGA GATCCGGCAC ACTGCGGACC GCTGGCGCGT GTCCCTGGAT 
GTCAACCACT TCGCCCCGGA CGAGCTGACG GTCAAGACCA AGGATGGCGT GGTGGAGATC 
ACCGGCAAGC ACGAGGAGCG GCAGGACGAG CATGGCTACA TCTCCCGGTG CTTCACGCGG 
AAATACACGC TGCCCCCCGG TGTGGACCCC ACCCAAGTTT CCTCCTCCCT GTCCCCTGAG 
GGCACACTGA CCGTGGAGGC CCCCATGCCC AAGCTAGCCA CGCAGTCCAA CGAGATCACC 
ATCCCAGTCA CCTTCGAGTC GCGGGCCCAG CTTGGGGGCC CAGAAGCTGC AAAATCCGAT 
GAGACTGCCG CCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001531.1
CDS	156..773
Translation	MTERRVPFSLLRGPSWDPFRDWYPHSRLFDQAFGLPRLPEEWSQWLGGSSWPGYVRPLPPAAIESPAVAAPAYSRALSRQLSSGVSEIRHTADRWRVSLDVNHFAPDELTVKTKDGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDPTQVSSSLSPEGTLTVEAPMPKLATQSNEITIPVTFESRAQLGGPEAAKSDETAAK

Target ORF information:

RefSeq Version	NM_001540.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens heat shock protein family B (small) member 1 (HSPB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001540.3

ORF Insert Sequence:

ATGACCGAGC GCCGCGTCCC CTTCTCGCTC CTGCGGGGCC CCAGCTGGGA CCCCTTCCGC 
GACTGGTACC CGCATAGCCG CCTCTTCGAC CAGGCCTTCG GGCTGCCCCG GCTGCCGGAG 
GAGTGGTCGC AGTGGTTAGG CGGCAGCAGC TGGCCAGGCT ACGTGCGCCC CCTGCCCCCC 
GCCGCCATCG AGAGCCCCGC AGTGGCCGCG CCCGCCTACA GCCGCGCGCT CAGCCGGCAA 
CTCAGCAGCG GGGTCTCGGA GATCCGGCAC ACTGCGGACC GCTGGCGCGT GTCCCTGGAT 
GTCAACCACT TCGCCCCGGA CGAGCTGACG GTCAAGACCA AGGATGGCGT GGTGGAGATC 
ACCGGCAAGC ACGAGGAGCG GCAGGACGAG CATGGCTACA TCTCCCGGTG CTTCACGCGG 
AAATACACGC TGCCCCCCGG TGTGGACCCC ACCCAAGTTT CCTCCTCCCT GTCCCCTGAG 
GGCACACTGA CCGTGGAGGC CCCCATGCCC AAGCTAGCCA CGCAGTCCAA CGAGATCACC 
ATCCCAGTCA CCTTCGAGTC GCGGGCCCAG CTTGGGGGCC CAGAAGCTGC AAAATCCGAT 
GAGACTGCCG CCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPB1 ( NM_001540.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001531.1 Homo sapiens heat shock protein family B (small) member 1 (HSPB1), mRNA.

Price & Availability↑

CloneID	OHu26288
Clone ID Related Accession (Same CDS sequence)	NM_001540.4 , NM_001540.3 , NM_001540.5
Accession Version	NM_001540.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	heat shock protein beta-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP231480.1 and BC012768.2. On Nov 22, 2018 this sequence version replaced NM_001540.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012292.1, X54079.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000248553.7/ ENSP00000248553.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCGAGC GCCGCGTCCC CTTCTCGCTC CTGCGGGGCC CCAGCTGGGA CCCCTTCCGC 
GACTGGTACC CGCATAGCCG CCTCTTCGAC CAGGCCTTCG GGCTGCCCCG GCTGCCGGAG 
GAGTGGTCGC AGTGGTTAGG CGGCAGCAGC TGGCCAGGCT ACGTGCGCCC CCTGCCCCCC 
GCCGCCATCG AGAGCCCCGC AGTGGCCGCG CCCGCCTACA GCCGCGCGCT CAGCCGGCAA 
CTCAGCAGCG GGGTCTCGGA GATCCGGCAC ACTGCGGACC GCTGGCGCGT GTCCCTGGAT 
GTCAACCACT TCGCCCCGGA CGAGCTGACG GTCAAGACCA AGGATGGCGT GGTGGAGATC 
ACCGGCAAGC ACGAGGAGCG GCAGGACGAG CATGGCTACA TCTCCCGGTG CTTCACGCGG 
AAATACACGC TGCCCCCCGG TGTGGACCCC ACCCAAGTTT CCTCCTCCCT GTCCCCTGAG 
GGCACACTGA CCGTGGAGGC CCCCATGCCC AAGCTAGCCA CGCAGTCCAA CGAGATCACC 
ATCCCAGTCA CCTTCGAGTC GCGGGCCCAG CTTGGGGGCC CAGAAGCTGC AAAATCCGAT 
GAGACTGCCG CCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001531.1
CDS	41..658
Translation	MTERRVPFSLLRGPSWDPFRDWYPHSRLFDQAFGLPRLPEEWSQWLGGSSWPGYVRPLPPAAIESPAVAAPAYSRALSRQLSSGVSEIRHTADRWRVSLDVNHFAPDELTVKTKDGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDPTQVSSSLSPEGTLTVEAPMPKLATQSNEITIPVTFESRAQLGGPEAAKSDETAAK

Target ORF information:

RefSeq Version	NM_001540.5
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock protein family B (small) member 1 (HSPB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001540.5

ORF Insert Sequence:

ATGACCGAGC GCCGCGTCCC CTTCTCGCTC CTGCGGGGCC CCAGCTGGGA CCCCTTCCGC 
GACTGGTACC CGCATAGCCG CCTCTTCGAC CAGGCCTTCG GGCTGCCCCG GCTGCCGGAG 
GAGTGGTCGC AGTGGTTAGG CGGCAGCAGC TGGCCAGGCT ACGTGCGCCC CCTGCCCCCC 
GCCGCCATCG AGAGCCCCGC AGTGGCCGCG CCCGCCTACA GCCGCGCGCT CAGCCGGCAA 
CTCAGCAGCG GGGTCTCGGA GATCCGGCAC ACTGCGGACC GCTGGCGCGT GTCCCTGGAT 
GTCAACCACT TCGCCCCGGA CGAGCTGACG GTCAAGACCA AGGATGGCGT GGTGGAGATC 
ACCGGCAAGC ACGAGGAGCG GCAGGACGAG CATGGCTACA TCTCCCGGTG CTTCACGCGG 
AAATACACGC TGCCCCCCGG TGTGGACCCC ACCCAAGTTT CCTCCTCCCT GTCCCCTGAG 
GGCACACTGA CCGTGGAGGC CCCCATGCCC AAGCTAGCCA CGCAGTCCAA CGAGATCACC 
ATCCCAGTCA CCTTCGAGTC GCGGGCCCAG CTTGGGGGCC CAGAAGCTGC AAAATCCGAT 
GAGACTGCCG CCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The expression and correlation of Hsp 70 and Hsp 27 in serous middle ear effusion fluids of pediatric patients-a preliminary study.
International journal of pediatric otorhinolaryngology101145-149(2017 Oct)
Min HJ,Choe JW,Chang MY,Kim KS,Lee SY,Mun SK

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HSPB1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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