HSPA8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSPA8
Entrez Gene ID	3312
Full Name	heat shock protein family A (Hsp70) member 8
Synonyms	HEL-33,HEL-S-72p,HSC54,HSC70,HSC71,HSP71,HSP73,HSPA10,LAP-1,LAP1,NIP71
General protein information	Preferred Names heat shock protein family A (Hsp70) member 8 Names heat shock cognate 71 kDa protein LPS-associated protein 1 N-myristoyltransferase inhibitor protein 71 constitutive heat shock protein 70 epididymis luminal protein 33 epididymis secretory sperm binding protein Li 72p heat shock 70kDa protein 8 heat shock 70kd protein 10 heat shock cognate protein 54 lipopolysaccharide-associated protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q24.1
Summary	This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011].
Disorder MIM:	600816

mRNA and Protein(s)

mRNA	Protein	Name
NM_153201.3	NP_694881.1	heat shock cognate 71 kDa protein isoform 2
NM_153201.4	NP_694881.1	heat shock cognate 71 kDa protein isoform 2
XM_011542798.1	XP_011541100.1	heat shock cognate 71 kDa protein isoform X1
NM_006597.5	NP_006588.1	heat shock cognate 71 kDa protein isoform 1
NM_006597.6	NP_006588.1	heat shock cognate 71 kDa protein isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HSPA8	NP_006588.1
	HSPA8	XP_002799868.1
Canis lupus familiaris (dog)	HSPA8	XP_005619707.1
Bos taurus (cattle)	HSPA8	NP_776770.2
Rattus norvegicus (Norway rat)	Hspa8	NP_077327.1
Gallus gallus (chicken)	HSPA8	NP_990334.1
Caenorhabditis elegans (roundworm)	hsp-1	NP_503068.1
Mus musculus (house mouse)	Hspa8	NP_112442.2
Danio rerio (zebrafish)	hspa8	NP_001103873.1
Xenopus tropicalis (tropical clawed frog)	hspa8	XP_004916187.1
Pan troglodytes (chimpanzee)	HSPA8	XP_003313441.2
Drosophila melanogaster (fruit fly)	Hsc70-1	NP_524063.1
Arabidopsis thaliana (thale cress)	AT3G09440	NP_001189847.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004944	XP_315042.3

Gene Ontology

The following HSPA8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSPA8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27538	NM_006597.6 Latest version!	Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu27538	XM_011542798.1 Latest version!	Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu27531	NM_153201.4 Latest version!	Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu27531	NM_153201.3	Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu27538	NM_006597.5	Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSPA8 ( NM_006597.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_006588.1 Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27538
Clone ID Related Accession (Same CDS sequence)	XM_011542798.1 , NM_006597.5 , NM_006597.6
Accession Version	NM_006597.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	heat shock cognate 71 kDa protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC312904.1, DC395565.1, BC019816.2 and AK222628.1. This sequence is a reference standard in the RefSeqGene project. On Jul 30, 2013 this sequence version replaced NM_006597.4. Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ224294.1, SRR1803612.77063.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC 
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT 
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG 
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT 
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC 
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT 
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT 
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG 
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG 
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT 
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA 
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC 
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC 
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC 
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT 
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT 
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG 
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC 
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT 
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT 
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA 
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT 
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG 
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG 
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT 
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC 
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG 
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG 
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA 
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC 
ACCATTGAAG AGGTTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006588.1
CDS	278..2218
Translation	MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD

Target ORF information:

RefSeq Version	NM_006597.5
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006597.5

ORF Insert Sequence:

ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC 
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT 
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG 
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT 
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC 
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT 
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT 
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG 
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG 
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT 
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA 
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC 
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC 
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC 
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT 
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT 
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG 
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC 
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT 
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT 
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA 
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT 
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG 
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG 
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT 
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC 
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG 
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG 
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA 
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC 
ACCATTGAAG AGGTTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPA8 ( NM_006597.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_006588.1 Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27538
Clone ID Related Accession (Same CDS sequence)	XM_011542798.1 , NM_006597.5 , NM_006597.6
Accession Version	NM_006597.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	heat shock cognate 71 kDa protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395565.1, BC019816.2, AK222628.1 and AP000926.6. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_006597.5. Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1011269.1, SRR3476690.494591.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000534624.6/ ENSP00000432083.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC 
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT 
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG 
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT 
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC 
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT 
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT 
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG 
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG 
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT 
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA 
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC 
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC 
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC 
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT 
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT 
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG 
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC 
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT 
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT 
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA 
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT 
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG 
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG 
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT 
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC 
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG 
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG 
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA 
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC 
ACCATTGAAG AGGTTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006588.1
CDS	79..2019
Translation	MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD

Target ORF information:

RefSeq Version	NM_006597.6
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006597.6

ORF Insert Sequence:

ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC 
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT 
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG 
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT 
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC 
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT 
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT 
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG 
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG 
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT 
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA 
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC 
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC 
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC 
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT 
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT 
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG 
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC 
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT 
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT 
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA 
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT 
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG 
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG 
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT 
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC 
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG 
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG 
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA 
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC 
ACCATTGAAG AGGTTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPA8 ( XM_011542798.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541100.1 Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu27538
Clone ID Related Accession (Same CDS sequence)	XM_011542798.1 , NM_006597.5 , NM_006597.6
Accession Version	XM_011542798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	heat shock cognate 71 kDa protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC 
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT 
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG 
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT 
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC 
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT 
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT 
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG 
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG 
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT 
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA 
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC 
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC 
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC 
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT 
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT 
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG 
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC 
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT 
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT 
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA 
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT 
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG 
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG 
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT 
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC 
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG 
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG 
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA 
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC 
ACCATTGAAG AGGTTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541100.1
CDS	26..1966
Translation	MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD

Target ORF information:

RefSeq Version	XM_011542798.1
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542798.1

ORF Insert Sequence:

ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC 
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT 
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG 
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT 
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC 
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT 
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT 
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG 
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG 
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT 
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA 
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC 
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC 
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC 
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT 
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT 
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG 
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC 
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT 
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT 
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA 
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT 
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG 
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG 
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT 
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC 
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG 
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG 
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA 
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC 
ACCATTGAAG AGGTTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPA8 ( NM_153201.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_694881.1 Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27531
Clone ID Related Accession (Same CDS sequence)	NM_153201.3 , NM_153201.4
Accession Version	NM_153201.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	heat shock cognate 71 kDa protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC312904.1, DC395565.1, AB034951.1 and AK222628.1. On or before Aug 31, 2013 this sequence version replaced XM_005271538.1, NM_153201.2. Transcript Variant: This variant (2) uses alternate splice sites, and is missing an exon in the 3' coding region compared to variant 1. However, it maintains the reading frame, and encodes a shorter isoform (2) missing an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222628.1, SRR1163657.50984.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC 
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT 
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG 
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT 
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC 
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT 
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT 
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG 
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG 
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT 
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA 
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC 
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC 
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC 
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT 
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT 
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG 
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC 
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT 
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT 
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA 
CTCACAGGCA TGCCAGGAGG AATGCCTGGG GGATTTCCTG GTGGTGGAGC TCCTCCCTCT 
GGTGGTGCTT CCTCAGGGCC CACCATTGAA GAGGTTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694881.1
CDS	278..1759
Translation	MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD

Target ORF information:

RefSeq Version	NM_153201.3
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153201.3

ORF Insert Sequence:

ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC 
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT 
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG 
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT 
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC 
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT 
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT 
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG 
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG 
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT 
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA 
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC 
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC 
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC 
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT 
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT 
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG 
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC 
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT 
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT 
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA 
CTCACAGGCA TGCCAGGAGG AATGCCTGGG GGATTTCCTG GTGGTGGAGC TCCTCCCTCT 
GGTGGTGCTT CCTCAGGGCC CACCATTGAA GAGGTTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPA8 ( NM_153201.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_694881.1 Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27531
Clone ID Related Accession (Same CDS sequence)	NM_153201.3 , NM_153201.4
Accession Version	NM_153201.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	heat shock cognate 71 kDa protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395565.1, AB034951.1, AK222628.1 and AP000926.6. On May 31, 2019 this sequence version replaced NM_153201.3. Transcript Variant: This variant (2) uses alternate splice sites, and is missing an exon in the 3' coding region compared to variant 1. However, it maintains the reading frame, and encodes a shorter isoform (2) missing an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222628.1, SRR1163658.366593.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC 
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT 
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG 
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT 
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC 
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT 
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT 
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG 
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG 
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT 
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA 
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC 
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC 
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC 
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT 
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT 
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG 
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC 
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT 
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT 
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA 
CTCACAGGCA TGCCAGGAGG AATGCCTGGG GGATTTCCTG GTGGTGGAGC TCCTCCCTCT 
GGTGGTGCTT CCTCAGGGCC CACCATTGAA GAGGTTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694881.1
CDS	79..1560
Translation	MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD

Target ORF information:

RefSeq Version	NM_153201.4
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153201.4

ORF Insert Sequence:

ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC 
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT 
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG 
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT 
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC 
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT 
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT 
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG 
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG 
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT 
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA 
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC 
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC 
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC 
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT 
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT 
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG 
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC 
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT 
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT 
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA 
CTCACAGGCA TGCCAGGAGG AATGCCTGGG GGATTTCCTG GTGGTGGAGC TCCTCCCTCT 
GGTGGTGCTT CCTCAGGGCC CACCATTGAA GAGGTTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HSPA8 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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