DNAJB2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNAJB2
Entrez Gene ID	3300
Full Name	DnaJ heat shock protein family (Hsp40) member B2
Synonyms	CMT2T,DSMA5,HSJ-1,HSJ1,HSPF3
General protein information	Preferred Names DnaJ heat shock protein family (Hsp40) member B2 Names dnaJ homolog subfamily B member 2 DnaJ (Hsp40) homolog, subfamily B, member 2 dnaJ protein homolog 1 heat shock 40 kDa protein 3 heat shock protein J1 heat shock protein, neuronal DNAJ-like 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q35
Summary	This gene is almost exclusively expressed in the brain, mainly in the neuronal layers. It encodes a protein that shows sequence similarity to bacterial DnaJ protein and the yeast homologs. In bacteria, this protein is implicated in protein folding and protein complex dissociation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011].
Disorder MIM:	604139
Disorder Html:	Spinal muscular atrophy, distal, autosomal recessive, 5, 614881 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_006736.5	NP_006727.2	dnaJ homolog subfamily B member 2 isoform b
NM_001039550.1	NP_001034639.1	dnaJ homolog subfamily B member 2 isoform a
NM_006736.6	NP_006727.2	dnaJ homolog subfamily B member 2 isoform b
NM_001039550.2	NP_001034639.1	dnaJ homolog subfamily B member 2 isoform a

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DNAJB2	XP_001147180.2
Canis lupus familiaris (dog)	DNAJB2	XP_003434299.1
Danio rerio (zebrafish)	dnajb2	NP_001073462.1
Drosophila melanogaster (fruit fly)	mrj	NP_725541.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012194	XP_320338.4
	DNAJB2	XP_002799104.1
Rattus norvegicus (Norway rat)	Dnajb2	NP_001103011.1
Gallus gallus (chicken)	DNAJB2	XP_424624.4
Xenopus tropicalis (tropical clawed frog)	dnajb2	NP_001015737.1
Homo sapiens (human)	DNAJB2	NP_006727.2
Bos taurus (cattle)	DNAJB2	NP_001029764.1
Mus musculus (house mouse)	Dnajb2	NP_001153355.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein kinase CK2 modulates HSJ1 function through phosphorylation of the UIM2 domain.
Ottaviani D, Marin O, Arrigoni G, Franchin C, Vilardell J, Sandre M, Li W, Parfitt DA, Pinna LA, Cheetham ME, Ruzzene M
Human molecular genetics26(3)611-623(2017 02)

Identification of a Large DNAJB2 Deletion in a Family with Spinal Muscular Atrophy and Parkinsonism.
Sanchez E, Darvish H, Mesias R, Taghavi S, Firouzabadi SG, Walker RH, Tafakhori A, Pais?n-Ruiz C
Human mutation37(11)1180-1189(2016 11)

HSJ1-related hereditary neuropathies: novel mutations and extended clinical spectrum.
Gess B, Auer-Grumbach M, Schirmacher A, Strom T, Zitzelsberger M, Rudnik-Sch?neborn S, R?hr D, Halfter H, Young P, Senderek J
Neurology83(19)1726-32(2014 Nov)

Common genetic variants associated with cognitive performance identified using the proxy-phenotype method.
Rietveld CA, Esko T, Davies G, Pers TH, Turley P, Benyamin B, Chabris CF, Emilsson V, Johnson AD, Lee JJ, de Leeuw C, Marioni RE, Medland SE, Miller MB, Rostapshova O, van der Lee SJ, Vinkhuyzen AA, Amin N, Conley D, Derringer J, van Duijn CM, Fehrmann R, Franke L, Glaeser EL, Hansell NK, Hayward C, Iacono WG, Ibrahim-Verbaas C, Jaddoe V, Karjalainen J, Laibson D, Lichtenstein P, Liewald DC, Magnusson PK, Martin NG, McGue M, McMahon G, Pedersen NL, Pinker S, Porteous DJ, Posthuma D, Rivadeneira F, Smith BH, Starr JM, Tiemeier H, Timpson NJ, Trzaskowski M, Uitterlinden AG, Verhulst FC, Ward ME, Wright MJ, Davey Smith G, Deary IJ, Johannesson M, Plomin R, Visscher PM, Benjamin DJ, Cesarini D, Koellinger PD
Proceedings of the National Academy of Sciences of the United States of America111(38)13790-4(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results disclose a novel interplay between ubiquitin- and phosphorylation-dependent signalling, and represent the first report of a regulatory mechanism for UIM-dependent function. They also suggest that CK2 inhibitors could release the full neuroprotective potential of HSJ1, and deserve future interest as therapeutic strategies for neurodegenerative disease.
Title: Protein kinase CK2 modulates HSJ1 function through phosphorylation of the UIM2 domain.

Study describes the identi fi cation of the fi rst deletion reported at the DNAJB2 locus, further expanding its phenotypic and genotypic spectrums as well as its disease-associated mechanisms with spinal muscular atrophy and parkinsonism.
Title: Identification of a Large DNAJB2 Deletion in a Family with Spinal Muscular Atrophy and Parkinsonism.

The results of this study confirm that HSJ1 mutations are a rare but detectable cause of autosomal recessive dHMN and CMT2.
Title: HSJ1-related hereditary neuropathies: novel mutations and extended clinical spectrum.

HSJ1a acts on mutant SOD1 through a combination of chaperone, co-chaperone and pro-ubiquitylation activity.
Title: Molecular chaperone mediated late-stage neuroprotection in the SOD1(G93A) mouse model of amyotrophic lateral sclerosis.

a mutation causing a loss-of-function of HSJ1 is linked to a pure lower motor neuron disease, strongly suggesting that HSJ1 also plays an important and specific role in motor neurons.
Title: A rare recessive distal hereditary motor neuropathy with HSJ1 chaperone mutation.

The following DNAJB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNAJB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22238	NM_001039550.2 Latest version!	Homo sapiens DnaJ heat shock protein family (Hsp40) member B2 (DNAJB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu22883	NM_006736.6 Latest version!	Homo sapiens DnaJ heat shock protein family (Hsp40) member B2 (DNAJB2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu22883	NM_006736.5	Homo sapiens DnaJ heat shock protein family (Hsp40) member B2 (DNAJB2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22238	NM_001039550.1	Homo sapiens DnaJ heat shock protein family (Hsp40) member B2 (DNAJB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNAJB2 ( NM_001039550.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001034639.1 Homo sapiens DnaJ heat shock protein family (Hsp40) member B2 (DNAJB2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22238
Clone ID Related Accession (Same CDS sequence)	NM_001039550.1 , NM_001039550.2
Accession Version	NM_001039550.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	dnaJ homolog subfamily B member 2 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL547521.3, BX420085.2, AC114803.5, BC047056.1 and BM689272.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279864.5051.1, ERR279873.3476.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATCCT ACTACGAGAT CCTAGACGTG CCGCGAAGTG CGTCCGCTGA TGACATCAAG 
AAGGCGTATC GGCGCAAGGC TCTCCAGTGG CACCCAGACA AAAACCCAGA TAATAAAGAG 
TTTGCTGAGA AGAAATTTAA GGAGGTGGCC GAGGCATATG AAGTGCTGTC TGACAAGCAC 
AAGCGGGAGA TTTACGACCG CTATGGCCGG GAAGGGCTGA CAGGGACAGG AACTGGCCCA 
TCTCGGGCAG AAGCTGGCAG TGGTGGGCCT GGCTTCACCT TCACCTTCCG CAGCCCCGAG 
GAGGTCTTCC GGGAATTCTT TGGGAGTGGA GACCCTTTTG CAGAGCTCTT TGATGACCTG 
GGCCCCTTCT CAGAGCTTCA GAACCGGGGT TCCCGACACT CAGGCCCCTT CTTTACCTTC 
TCTTCCTCCT TCCCTGGGCA CTCCGATTTC TCCTCCTCAT CTTTCTCCTT CAGTCCTGGG 
GCTGGTGCTT TTCGCTCTGT TTCTACATCT ACCACCTTTG TCCAAGGACG CCGCATCACC 
ACACGCAGAA TCATGGAGAA CGGGCAGGAG CGGGTGGAAG TGGAGGAGGA TGGGCAGCTG 
AAGTCAGTCA CAATCAATGG TGTCCCAGAT GACCTGGCAC TGGGCTTGGA GCTGAGCCGT 
CGCGAGCAGC AGCCGTCAGT CACTTCCAGG TCTGGGGGCA CTCAGGTCCA GCAGACCCCT 
GCCTCATGCC CCTTGGACAG CGACCTCTCT GAGGATGAGG ACCTGCAGCT GGCCATGGCC 
TACAGCCTGT CAGAGATGGA GGCAGCTGGG AAGAAACCCG CAGATGTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034639.1
CDS	238..1071
Translation	MASYYEILDVPRSASADDIKKAYRRKALQWHPDKNPDNKEFAEKKFKEVAEAYEVLSDKHKREIYDRYGREGLTGTGTGPSRAEAGSGGPGFTFTFRSPEEVFREFFGSGDPFAELFDDLGPFSELQNRGSRHSGPFFTFSSSFPGHSDFSSSSFSFSPGAGAFRSVSTSTTFVQGRRITTRRIMENGQERVEVEEDGQLKSVTINGVPDDLALGLELSRREQQPSVTSRSGGTQVQQTPASCPLDSDLSEDEDLQLAMAYSLSEMEAAGKKPADVF

Target ORF information:

RefSeq Version	NM_001039550.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DnaJ heat shock protein family (Hsp40) member B2 (DNAJB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039550.1

ORF Insert Sequence:

ATGGCATCCT ACTACGAGAT CCTAGACGTG CCGCGAAGTG CGTCCGCTGA TGACATCAAG 
AAGGCGTATC GGCGCAAGGC TCTCCAGTGG CACCCAGACA AAAACCCAGA TAATAAAGAG 
TTTGCTGAGA AGAAATTTAA GGAGGTGGCC GAGGCATATG AAGTGCTGTC TGACAAGCAC 
AAGCGGGAGA TTTACGACCG CTATGGCCGG GAAGGGCTGA CAGGGACAGG AACTGGCCCA 
TCTCGGGCAG AAGCTGGCAG TGGTGGGCCT GGCTTCACCT TCACCTTCCG CAGCCCCGAG 
GAGGTCTTCC GGGAATTCTT TGGGAGTGGA GACCCTTTTG CAGAGCTCTT TGATGACCTG 
GGCCCCTTCT CAGAGCTTCA GAACCGGGGT TCCCGACACT CAGGCCCCTT CTTTACCTTC 
TCTTCCTCCT TCCCTGGGCA CTCCGATTTC TCCTCCTCAT CTTTCTCCTT CAGTCCTGGG 
GCTGGTGCTT TTCGCTCTGT TTCTACATCT ACCACCTTTG TCCAAGGACG CCGCATCACC 
ACACGCAGAA TCATGGAGAA CGGGCAGGAG CGGGTGGAAG TGGAGGAGGA TGGGCAGCTG 
AAGTCAGTCA CAATCAATGG TGTCCCAGAT GACCTGGCAC TGGGCTTGGA GCTGAGCCGT 
CGCGAGCAGC AGCCGTCAGT CACTTCCAGG TCTGGGGGCA CTCAGGTCCA GCAGACCCCT 
GCCTCATGCC CCTTGGACAG CGACCTCTCT GAGGATGAGG ACCTGCAGCT GGCCATGGCC 
TACAGCCTGT CAGAGATGGA GGCAGCTGGG AAGAAACCCG CAGATGTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAJB2 ( NM_001039550.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001034639.1 Homo sapiens DnaJ heat shock protein family (Hsp40) member B2 (DNAJB2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22238
Clone ID Related Accession (Same CDS sequence)	NM_001039550.1 , NM_001039550.2
Accession Version	NM_001039550.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	dnaJ homolog subfamily B member 2 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC114803.5 and BC047056.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001039550.1. Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.195532.1, SRR3476690.659824.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATCCT ACTACGAGAT CCTAGACGTG CCGCGAAGTG CGTCCGCTGA TGACATCAAG 
AAGGCGTATC GGCGCAAGGC TCTCCAGTGG CACCCAGACA AAAACCCAGA TAATAAAGAG 
TTTGCTGAGA AGAAATTTAA GGAGGTGGCC GAGGCATATG AAGTGCTGTC TGACAAGCAC 
AAGCGGGAGA TTTACGACCG CTATGGCCGG GAAGGGCTGA CAGGGACAGG AACTGGCCCA 
TCTCGGGCAG AAGCTGGCAG TGGTGGGCCT GGCTTCACCT TCACCTTCCG CAGCCCCGAG 
GAGGTCTTCC GGGAATTCTT TGGGAGTGGA GACCCTTTTG CAGAGCTCTT TGATGACCTG 
GGCCCCTTCT CAGAGCTTCA GAACCGGGGT TCCCGACACT CAGGCCCCTT CTTTACCTTC 
TCTTCCTCCT TCCCTGGGCA CTCCGATTTC TCCTCCTCAT CTTTCTCCTT CAGTCCTGGG 
GCTGGTGCTT TTCGCTCTGT TTCTACATCT ACCACCTTTG TCCAAGGACG CCGCATCACC 
ACACGCAGAA TCATGGAGAA CGGGCAGGAG CGGGTGGAAG TGGAGGAGGA TGGGCAGCTG 
AAGTCAGTCA CAATCAATGG TGTCCCAGAT GACCTGGCAC TGGGCTTGGA GCTGAGCCGT 
CGCGAGCAGC AGCCGTCAGT CACTTCCAGG TCTGGGGGCA CTCAGGTCCA GCAGACCCCT 
GCCTCATGCC CCTTGGACAG CGACCTCTCT GAGGATGAGG ACCTGCAGCT GGCCATGGCC 
TACAGCCTGT CAGAGATGGA GGCAGCTGGG AAGAAACCCG CAGATGTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034639.1
CDS	190..1023
Translation	MASYYEILDVPRSASADDIKKAYRRKALQWHPDKNPDNKEFAEKKFKEVAEAYEVLSDKHKREIYDRYGREGLTGTGTGPSRAEAGSGGPGFTFTFRSPEEVFREFFGSGDPFAELFDDLGPFSELQNRGSRHSGPFFTFSSSFPGHSDFSSSSFSFSPGAGAFRSVSTSTTFVQGRRITTRRIMENGQERVEVEEDGQLKSVTINGVPDDLALGLELSRREQQPSVTSRSGGTQVQQTPASCPLDSDLSEDEDLQLAMAYSLSEMEAAGKKPADVF

Target ORF information:

RefSeq Version	NM_001039550.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DnaJ heat shock protein family (Hsp40) member B2 (DNAJB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039550.2

ORF Insert Sequence:

ATGGCATCCT ACTACGAGAT CCTAGACGTG CCGCGAAGTG CGTCCGCTGA TGACATCAAG 
AAGGCGTATC GGCGCAAGGC TCTCCAGTGG CACCCAGACA AAAACCCAGA TAATAAAGAG 
TTTGCTGAGA AGAAATTTAA GGAGGTGGCC GAGGCATATG AAGTGCTGTC TGACAAGCAC 
AAGCGGGAGA TTTACGACCG CTATGGCCGG GAAGGGCTGA CAGGGACAGG AACTGGCCCA 
TCTCGGGCAG AAGCTGGCAG TGGTGGGCCT GGCTTCACCT TCACCTTCCG CAGCCCCGAG 
GAGGTCTTCC GGGAATTCTT TGGGAGTGGA GACCCTTTTG CAGAGCTCTT TGATGACCTG 
GGCCCCTTCT CAGAGCTTCA GAACCGGGGT TCCCGACACT CAGGCCCCTT CTTTACCTTC 
TCTTCCTCCT TCCCTGGGCA CTCCGATTTC TCCTCCTCAT CTTTCTCCTT CAGTCCTGGG 
GCTGGTGCTT TTCGCTCTGT TTCTACATCT ACCACCTTTG TCCAAGGACG CCGCATCACC 
ACACGCAGAA TCATGGAGAA CGGGCAGGAG CGGGTGGAAG TGGAGGAGGA TGGGCAGCTG 
AAGTCAGTCA CAATCAATGG TGTCCCAGAT GACCTGGCAC TGGGCTTGGA GCTGAGCCGT 
CGCGAGCAGC AGCCGTCAGT CACTTCCAGG TCTGGGGGCA CTCAGGTCCA GCAGACCCCT 
GCCTCATGCC CCTTGGACAG CGACCTCTCT GAGGATGAGG ACCTGCAGCT GGCCATGGCC 
TACAGCCTGT CAGAGATGGA GGCAGCTGGG AAGAAACCCG CAGATGTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAJB2 ( NM_006736.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_006727.2 Homo sapiens DnaJ heat shock protein family (Hsp40) member B2 (DNAJB2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22883
Clone ID Related Accession (Same CDS sequence)	NM_006736.5 , NM_006736.6
Accession Version	NM_006736.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Homo sapiens(human)
Product	dnaJ homolog subfamily B member 2 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL547521.3, BX420085.2, AC114803.5, BT007088.1, BX478051.1, CA392563.1, BF795662.1, BC047056.1 and BM689272.1. On Feb 20, 2006 this sequence version replaced NM_006736.4. Transcript Variant: This variant (2) is alternatively spliced at the 3' end compared to variant 1. This results in a longer isoform (b) with a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011609.2, SRR1660807.34561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATCCT ACTACGAGAT CCTAGACGTG CCGCGAAGTG CGTCCGCTGA TGACATCAAG 
AAGGCGTATC GGCGCAAGGC TCTCCAGTGG CACCCAGACA AAAACCCAGA TAATAAAGAG 
TTTGCTGAGA AGAAATTTAA GGAGGTGGCC GAGGCATATG AAGTGCTGTC TGACAAGCAC 
AAGCGGGAGA TTTACGACCG CTATGGCCGG GAAGGGCTGA CAGGGACAGG AACTGGCCCA 
TCTCGGGCAG AAGCTGGCAG TGGTGGGCCT GGCTTCACCT TCACCTTCCG CAGCCCCGAG 
GAGGTCTTCC GGGAATTCTT TGGGAGTGGA GACCCTTTTG CAGAGCTCTT TGATGACCTG 
GGCCCCTTCT CAGAGCTTCA GAACCGGGGT TCCCGACACT CAGGCCCCTT CTTTACCTTC 
TCTTCCTCCT TCCCTGGGCA CTCCGATTTC TCCTCCTCAT CTTTCTCCTT CAGTCCTGGG 
GCTGGTGCTT TTCGCTCTGT TTCTACATCT ACCACCTTTG TCCAAGGACG CCGCATCACC 
ACACGCAGAA TCATGGAGAA CGGGCAGGAG CGGGTGGAAG TGGAGGAGGA TGGGCAGCTG 
AAGTCAGTCA CAATCAATGG TGTCCCAGAT GACCTGGCAC TGGGCTTGGA GCTGAGCCGT 
CGCGAGCAGC AGCCGTCAGT CACTTCCAGG TCTGGGGGCA CTCAGGTCCA GCAGACCCCT 
GCCTCATGCC CCTTGGACAG CGACCTCTCT GAGGATGAGG ACCTGCAGCT GGCCATGGCC 
TACAGCCTGT CAGAGATGGA GGCAGCTGGG AAGAAACCCG CAGGTGGGCG GGAGGCACAG 
CACCGACGGC AGGGGCGGCC CAAGGCCCAG CACCAAGATC CAGGCTTGGG GGGGACCCAG 
GAGGGTGCGA GGGGTGAAGC AACCAAACGC AGTCCATCCC CAGAGGAGAA GGCCTCTCGC 
TGCCTCATCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006727.2
CDS	238..1212
Translation	MASYYEILDVPRSASADDIKKAYRRKALQWHPDKNPDNKEFAEKKFKEVAEAYEVLSDKHKREIYDRYGREGLTGTGTGPSRAEAGSGGPGFTFTFRSPEEVFREFFGSGDPFAELFDDLGPFSELQNRGSRHSGPFFTFSSSFPGHSDFSSSSFSFSPGAGAFRSVSTSTTFVQGRRITTRRIMENGQERVEVEEDGQLKSVTINGVPDDLALGLELSRREQQPSVTSRSGGTQVQQTPASCPLDSDLSEDEDLQLAMAYSLSEMEAAGKKPAGGREAQHRRQGRPKAQHQDPGLGGTQEGARGEATKRSPSPEEKASRCLIL

Target ORF information:

RefSeq Version	NM_006736.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DnaJ heat shock protein family (Hsp40) member B2 (DNAJB2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006736.5

ORF Insert Sequence:

ATGGCATCCT ACTACGAGAT CCTAGACGTG CCGCGAAGTG CGTCCGCTGA TGACATCAAG 
AAGGCGTATC GGCGCAAGGC TCTCCAGTGG CACCCAGACA AAAACCCAGA TAATAAAGAG 
TTTGCTGAGA AGAAATTTAA GGAGGTGGCC GAGGCATATG AAGTGCTGTC TGACAAGCAC 
AAGCGGGAGA TTTACGACCG CTATGGCCGG GAAGGGCTGA CAGGGACAGG AACTGGCCCA 
TCTCGGGCAG AAGCTGGCAG TGGTGGGCCT GGCTTCACCT TCACCTTCCG CAGCCCCGAG 
GAGGTCTTCC GGGAATTCTT TGGGAGTGGA GACCCTTTTG CAGAGCTCTT TGATGACCTG 
GGCCCCTTCT CAGAGCTTCA GAACCGGGGT TCCCGACACT CAGGCCCCTT CTTTACCTTC 
TCTTCCTCCT TCCCTGGGCA CTCCGATTTC TCCTCCTCAT CTTTCTCCTT CAGTCCTGGG 
GCTGGTGCTT TTCGCTCTGT TTCTACATCT ACCACCTTTG TCCAAGGACG CCGCATCACC 
ACACGCAGAA TCATGGAGAA CGGGCAGGAG CGGGTGGAAG TGGAGGAGGA TGGGCAGCTG 
AAGTCAGTCA CAATCAATGG TGTCCCAGAT GACCTGGCAC TGGGCTTGGA GCTGAGCCGT 
CGCGAGCAGC AGCCGTCAGT CACTTCCAGG TCTGGGGGCA CTCAGGTCCA GCAGACCCCT 
GCCTCATGCC CCTTGGACAG CGACCTCTCT GAGGATGAGG ACCTGCAGCT GGCCATGGCC 
TACAGCCTGT CAGAGATGGA GGCAGCTGGG AAGAAACCCG CAGGTGGGCG GGAGGCACAG 
CACCGACGGC AGGGGCGGCC CAAGGCCCAG CACCAAGATC CAGGCTTGGG GGGGACCCAG 
GAGGGTGCGA GGGGTGAAGC AACCAAACGC AGTCCATCCC CAGAGGAGAA GGCCTCTCGC 
TGCCTCATCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAJB2 ( NM_006736.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_006727.2 Homo sapiens DnaJ heat shock protein family (Hsp40) member B2 (DNAJB2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22883
Clone ID Related Accession (Same CDS sequence)	NM_006736.5 , NM_006736.6
Accession Version	NM_006736.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	dnaJ homolog subfamily B member 2 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC114803.5, BT007088.1, BX478051.1, CA392563.1, BF795662.1 and BC047056.1. On Jun 2, 2019 this sequence version replaced NM_006736.5. Transcript Variant: This variant (2) is alternatively spliced at the 3' end compared to variant 1. This results in a longer isoform (b) with a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.213300.1, SRR3476690.105468.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCATCCT ACTACGAGAT CCTAGACGTG CCGCGAAGTG CGTCCGCTGA TGACATCAAG 
AAGGCGTATC GGCGCAAGGC TCTCCAGTGG CACCCAGACA AAAACCCAGA TAATAAAGAG 
TTTGCTGAGA AGAAATTTAA GGAGGTGGCC GAGGCATATG AAGTGCTGTC TGACAAGCAC 
AAGCGGGAGA TTTACGACCG CTATGGCCGG GAAGGGCTGA CAGGGACAGG AACTGGCCCA 
TCTCGGGCAG AAGCTGGCAG TGGTGGGCCT GGCTTCACCT TCACCTTCCG CAGCCCCGAG 
GAGGTCTTCC GGGAATTCTT TGGGAGTGGA GACCCTTTTG CAGAGCTCTT TGATGACCTG 
GGCCCCTTCT CAGAGCTTCA GAACCGGGGT TCCCGACACT CAGGCCCCTT CTTTACCTTC 
TCTTCCTCCT TCCCTGGGCA CTCCGATTTC TCCTCCTCAT CTTTCTCCTT CAGTCCTGGG 
GCTGGTGCTT TTCGCTCTGT TTCTACATCT ACCACCTTTG TCCAAGGACG CCGCATCACC 
ACACGCAGAA TCATGGAGAA CGGGCAGGAG CGGGTGGAAG TGGAGGAGGA TGGGCAGCTG 
AAGTCAGTCA CAATCAATGG TGTCCCAGAT GACCTGGCAC TGGGCTTGGA GCTGAGCCGT 
CGCGAGCAGC AGCCGTCAGT CACTTCCAGG TCTGGGGGCA CTCAGGTCCA GCAGACCCCT 
GCCTCATGCC CCTTGGACAG CGACCTCTCT GAGGATGAGG ACCTGCAGCT GGCCATGGCC 
TACAGCCTGT CAGAGATGGA GGCAGCTGGG AAGAAACCCG CAGGTGGGCG GGAGGCACAG 
CACCGACGGC AGGGGCGGCC CAAGGCCCAG CACCAAGATC CAGGCTTGGG GGGGACCCAG 
GAGGGTGCGA GGGGTGAAGC AACCAAACGC AGTCCATCCC CAGAGGAGAA GGCCTCTCGC 
TGCCTCATCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006727.2
CDS	190..1164
Translation	MASYYEILDVPRSASADDIKKAYRRKALQWHPDKNPDNKEFAEKKFKEVAEAYEVLSDKHKREIYDRYGREGLTGTGTGPSRAEAGSGGPGFTFTFRSPEEVFREFFGSGDPFAELFDDLGPFSELQNRGSRHSGPFFTFSSSFPGHSDFSSSSFSFSPGAGAFRSVSTSTTFVQGRRITTRRIMENGQERVEVEEDGQLKSVTINGVPDDLALGLELSRREQQPSVTSRSGGTQVQQTPASCPLDSDLSEDEDLQLAMAYSLSEMEAAGKKPAGGREAQHRRQGRPKAQHQDPGLGGTQEGARGEATKRSPSPEEKASRCLIL

Target ORF information:

RefSeq Version	NM_006736.6
Organism	Homo sapiens(human)
Definition	Homo sapiens DnaJ heat shock protein family (Hsp40) member B2 (DNAJB2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006736.6

ORF Insert Sequence:

ATGGCATCCT ACTACGAGAT CCTAGACGTG CCGCGAAGTG CGTCCGCTGA TGACATCAAG 
AAGGCGTATC GGCGCAAGGC TCTCCAGTGG CACCCAGACA AAAACCCAGA TAATAAAGAG 
TTTGCTGAGA AGAAATTTAA GGAGGTGGCC GAGGCATATG AAGTGCTGTC TGACAAGCAC 
AAGCGGGAGA TTTACGACCG CTATGGCCGG GAAGGGCTGA CAGGGACAGG AACTGGCCCA 
TCTCGGGCAG AAGCTGGCAG TGGTGGGCCT GGCTTCACCT TCACCTTCCG CAGCCCCGAG 
GAGGTCTTCC GGGAATTCTT TGGGAGTGGA GACCCTTTTG CAGAGCTCTT TGATGACCTG 
GGCCCCTTCT CAGAGCTTCA GAACCGGGGT TCCCGACACT CAGGCCCCTT CTTTACCTTC 
TCTTCCTCCT TCCCTGGGCA CTCCGATTTC TCCTCCTCAT CTTTCTCCTT CAGTCCTGGG 
GCTGGTGCTT TTCGCTCTGT TTCTACATCT ACCACCTTTG TCCAAGGACG CCGCATCACC 
ACACGCAGAA TCATGGAGAA CGGGCAGGAG CGGGTGGAAG TGGAGGAGGA TGGGCAGCTG 
AAGTCAGTCA CAATCAATGG TGTCCCAGAT GACCTGGCAC TGGGCTTGGA GCTGAGCCGT 
CGCGAGCAGC AGCCGTCAGT CACTTCCAGG TCTGGGGGCA CTCAGGTCCA GCAGACCCCT 
GCCTCATGCC CCTTGGACAG CGACCTCTCT GAGGATGAGG ACCTGCAGCT GGCCATGGCC 
TACAGCCTGT CAGAGATGGA GGCAGCTGGG AAGAAACCCG CAGGTGGGCG GGAGGCACAG 
CACCGACGGC AGGGGCGGCC CAAGGCCCAG CACCAAGATC CAGGCTTGGG GGGGACCCAG 
GAGGGTGCGA GGGGTGAAGC AACCAAACGC AGTCCATCCC CAGAGGAGAA GGCCTCTCGC 
TGCCTCATCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein kinase CK2 modulates HSJ1 function through phosphorylation of the UIM2 domain.
Human molecular genetics26(3)611-623(2017 02)
Ottaviani D,Marin O,Arrigoni G,Franchin C,Vilardell J,Sandre M,Li W,Parfitt DA,Pinna LA,Cheetham ME,Ruzzene M

Identification of a Large DNAJB2 Deletion in a Family with Spinal Muscular Atrophy and Parkinsonism.
Human mutation37(11)1180-1189(2016 11)
Sanchez E,Darvish H,Mesias R,Taghavi S,Firouzabadi SG,Walker RH,Tafakhori A,Pais?n-Ruiz C

HSJ1-related hereditary neuropathies: novel mutations and extended clinical spectrum.
Neurology83(19)1726-32(2014 Nov)
Gess B,Auer-Grumbach M,Schirmacher A,Strom T,Zitzelsberger M,Rudnik-Sch?neborn S,R?hr D,Halfter H,Young P,Senderek J

Common genetic variants associated with cognitive performance identified using the proxy-phenotype method.
Proceedings of the National Academy of Sciences of the United States of America111(38)13790-4(2014 Sep)
Rietveld CA,Esko T,Davies G,Pers TH,Turley P,Benyamin B,Chabris CF,Emilsson V,Johnson AD,Lee JJ,de Leeuw C,Marioni RE,Medland SE,Miller MB,Rostapshova O,van der Lee SJ,Vinkhuyzen AA,Amin N,Conley D,Derringer J,van Duijn CM,Fehrmann R,Franke L,Glaeser EL,Hansell NK,Hayward C,Iacono WG,Ibrahim-Verbaas C,Jaddoe V,Karjalainen J,Laibson D,Lichtenstein P,Liewald DC,Magnusson PK,Martin NG,McGue M,McMahon G,Pedersen NL,Pinker S,Porteous DJ,Posthuma D,Rivadeneira F,Smith BH,Starr JM,Tiemeier H,Timpson NJ,Trzaskowski M,Uitterlinden AG,Verhulst FC,Ward ME,Wright MJ,Davey Smith G,Deary IJ,Johannesson M,Plomin R,Visscher PM,Benjamin DJ,Cesarini D,Koellinger PD

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DNAJB2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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