HSBP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSBP1
Entrez Gene ID	3281
Full Name	heat shock factor binding protein 1
Synonyms	NPC-A-13
General protein information	Preferred Names heat shock factor binding protein 1 Names heat shock factor-binding protein 1 nasopharyngeal carcinoma-associated antigen 13
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q23.3
Summary	The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1(HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response. [provided by RefSeq, Jul 2008].
Disorder MIM:	604553

mRNA and Protein(s)

mRNA	Protein	Name
NM_001537.3	NP_001528.1	heat shock factor-binding protein 1
NM_001537.4	NP_001528.1	heat shock factor-binding protein 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Hsbp1	NP_077181.1
Gallus gallus (chicken)	HSBP1	NP_001106280.1
Rattus norvegicus (Norway rat)	Hsbp1	NP_775142.1
Xenopus tropicalis (tropical clawed frog)	hsbp1	NP_001011422.1
Pan troglodytes (chimpanzee)	HSBP1	XP_003952954.1
	HSBP1	XP_001112013.1
Canis lupus familiaris (dog)	HSBP1	XP_005620708.1
Bos taurus (cattle)	HSBP1	NP_001106787.1
Homo sapiens (human)	HSBP1	NP_001528.1
Danio rerio (zebrafish)	hsbp1a	NP_001166105.1

Gene Ontology
Bibliography

Related articles in PubMed

Nucleotide exchange factors Fes1 and HspBP1 mimic substrate to release misfolded proteins from Hsp70.
Gowda NKC, Kaimal JM, Kityk R, Daniel C, Liebau J, ?hman M, Mayer MP, Andr?asson C
Nature structural & molecular biology25(1)83-89(2018 Jan)

A novel p.T139M mutation in HSPB1 highlighting the phenotypic spectrum in a family.
Amornvit J, Yalvac ME, Chen L, Sahenk Z
Brain and behavior7(8)e00774(2017 Aug)

pH-dependent structural modulation is conserved in the human small heat shock protein HSBP1.
Clouser AF, Klevit RE
Cell stress & chaperones22(4)569-575(2017 Jul)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HSBP1 is crucial for regulating resistance to radiotherapy of oral squamous epithelial carcinoma cells by inducing stem-like status.
Title: Overexpression of HSBP1 is associated with resistance to radiotherapy in oral squamous epithelial carcinoma.

HspBP1 inhibited the cytotoxic activity of the Tag7-Hsp70 complex secreted by lymphocytes. HspBP1
Title: The heat shock-binding protein (HspBP1) protects cells against the cytotoxic action of the Tag7-Hsp70 complex.

This study reports the crystal structure of a degradation-resistant fragment of HSBP1. A continuous, long-helix conformation of HSBP1 assembles into a coiled-coil three-helix bundle and subsequently into an elongated, symmetrical hexamer.
Title: Crystal structure of the hexamer of human heat shock factor binding protein 1.

HSBP likely mediates its function through its trimerization domain.
Title: Structure-function analysis of the heat shock factor-binding protein reveals a protein composed solely of a highly conserved and dynamic coiled-coil trimerization domain.

The following HSBP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSBP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08535	NM_001537.4 Latest version!	Homo sapiens heat shock factor binding protein 1 (HSBP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu08535	NM_001537.3	Homo sapiens heat shock factor binding protein 1 (HSBP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSBP1 ( NM_001537.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001528.1 Homo sapiens heat shock factor binding protein 1 (HSBP1), mRNA.

Price & Availability↑

CloneID	OHu08535
Clone ID Related Accession (Same CDS sequence)	NM_001537.3 , NM_001537.4
Accession Version	NM_001537.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	heat shock factor-binding protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009119.10, BC007515.2, AK172732.1 and DB519225.1. On May 25, 2012 this sequence version replaced NM_001537.2. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.193817.1, SRR1803613.44363.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGA CTGACCCCAA GACCGTGCAG GACCTCACCT CGGTGGTGCA GACACTCCTG 
CAGCAGATGC AAGATAAATT TCAGACCATG TCTGACCAGA TCATTGGGAG AATTGATGAT 
ATGAGTAGTC GCATTGATGA TCTGGAAAAG AATATCGCGG ACCTCATGAC ACAGGCTGGG 
GTGGAAGAAC TGGAAAGTGA AAACAAGATA CCTGCCACGC AAAAGAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001528.1
CDS	175..405
Translation	MAETDPKTVQDLTSVVQTLLQQMQDKFQTMSDQIIGRIDDMSSRIDDLEKNIADLMTQAGVEELESENKIPATQKS

Target ORF information:

RefSeq Version	NM_001537.3
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock factor binding protein 1 (HSBP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001537.3

ORF Insert Sequence:

ATGGCCGAGA CTGACCCCAA GACCGTGCAG GACCTCACCT CGGTGGTGCA GACACTCCTG 
CAGCAGATGC AAGATAAATT TCAGACCATG TCTGACCAGA TCATTGGGAG AATTGATGAT 
ATGAGTAGTC GCATTGATGA TCTGGAAAAG AATATCGCGG ACCTCATGAC ACAGGCTGGG 
GTGGAAGAAC TGGAAAGTGA AAACAAGATA CCTGCCACGC AAAAGAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSBP1 ( NM_001537.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001528.1 Homo sapiens heat shock factor binding protein 1 (HSBP1), mRNA.

Price & Availability↑

CloneID	OHu08535
Clone ID Related Accession (Same CDS sequence)	NM_001537.3 , NM_001537.4
Accession Version	NM_001537.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	heat shock factor-binding protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009119.10, BC007515.2, AK172732.1 and DB519225.1. On May 17, 2019 this sequence version replaced NM_001537.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.38835.1, SRR1803613.41892.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000433866.7/ ENSP00000392896.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGA CTGACCCCAA GACCGTGCAG GACCTCACCT CGGTGGTGCA GACACTCCTG 
CAGCAGATGC AAGATAAATT TCAGACCATG TCTGACCAGA TCATTGGGAG AATTGATGAT 
ATGAGTAGTC GCATTGATGA TCTGGAAAAG AATATCGCGG ACCTCATGAC ACAGGCTGGG 
GTGGAAGAAC TGGAAAGTGA AAACAAGATA CCTGCCACGC AAAAGAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001528.1
CDS	100..330
Translation	MAETDPKTVQDLTSVVQTLLQQMQDKFQTMSDQIIGRIDDMSSRIDDLEKNIADLMTQAGVEELESENKIPATQKS

Target ORF information:

RefSeq Version	NM_001537.4
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock factor binding protein 1 (HSBP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001537.4

ORF Insert Sequence:

ATGGCCGAGA CTGACCCCAA GACCGTGCAG GACCTCACCT CGGTGGTGCA GACACTCCTG 
CAGCAGATGC AAGATAAATT TCAGACCATG TCTGACCAGA TCATTGGGAG AATTGATGAT 
ATGAGTAGTC GCATTGATGA TCTGGAAAAG AATATCGCGG ACCTCATGAC ACAGGCTGGG 
GTGGAAGAAC TGGAAAGTGA AAACAAGATA CCTGCCACGC AAAAGAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nucleotide exchange factors Fes1 and HspBP1 mimic substrate to release misfolded proteins from Hsp70.
Nature structural & molecular biology25(1)83-89(2018 Jan)
Gowda NKC,Kaimal JM,Kityk R,Daniel C,Liebau J,?hman M,Mayer MP,Andr?asson C

A novel p.T139M mutation in HSPB1 highlighting the phenotypic spectrum in a family.
Brain and behavior7(8)e00774(2017 Aug)
Amornvit J,Yalvac ME,Chen L,Sahenk Z

pH-dependent structural modulation is conserved in the human small heat shock protein HSBP1.
Cell stress & chaperones22(4)569-575(2017 Jul)
Clouser AF,Klevit RE

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

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HSBP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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