HLA-DOB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HLA-DOB
Entrez Gene ID	3112
Full Name	major histocompatibility complex, class II, DO beta
Synonyms	DOB,HLA_DOB
General protein information	Preferred Names major histocompatibility complex, class II, DO beta Names HLA class II histocompatibility antigen, DO beta chain MHC class II antigen DOB
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.32
Summary	HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008].
Disorder MIM:	600629

mRNA and Protein(s)

mRNA	Protein	Name
NM_002120.4	NP_002111.1	HLA class II histocompatibility antigen, DO beta chain precursor
NM_002120.3	NP_002111.1	HLA class II histocompatibility antigen, DO beta chain precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	HLA-DOB	NP_002111.1
Rattus norvegicus (Norway rat)	RT1-DOb	NP_001008846.1
Pan troglodytes (chimpanzee)	PATR-DOB	NP_001123943.1
	MAMU-DOB	XP_001115393.1
Canis lupus familiaris (dog)	DLA-DOB	NP_001041592.1
Bos taurus (cattle)	BOLA-DOB	NP_001013618.1
Mus musculus (house mouse)	H2-Ob	NP_034519.2

Gene Ontology
Bibliography

Related articles in PubMed

Neutralizing Antibody Responses to Viral Infections Are Linked to the Non-classical MHC Class II Gene H2-Ob.
Denzin LK, Khan AA, Virdis F, Wilks J, Kane M, Beilinson HA, Dikiy S, Case LK, Roopenian D, Witkowski M, Chervonsky AV, Golovkina TV
Immunity47(2)310-322.e7(2017 Aug)

Association of polymorphisms in HLA antigen presentation-related genes with the outcomes of HCV infection.
Huang P, Zhang Y, Lu X, Xu Y, Wang J, Zhang Y, Yu R, Su J
PloS one10(4)e0123513(2015)

Ubiquitination of HLA-DO by MARCH family E3 ligases.
Jahnke M, Trowsdale J, Kelly AP
European journal of immunology43(5)1153-61(2013 May)

A genome-wide association study identifies 2 susceptibility Loci for Crohn's disease in a Japanese population.
Yamazaki K, Umeno J, Takahashi A, Hirano A, Johnson TA, Kumasaka N, Morizono T, Hosono N, Kawaguchi T, Takazoe M, Yamada T, Suzuki Y, Tanaka H, Motoya S, Hosokawa M, Arimura Y, Shinomura Y, Matsui T, Matsumoto T, Iida M, Tsunoda T, Nakamura Y, Kamatani N, Kubo M
Gastroenterology144(4)781-8(2013 Apr)

HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism.
Guce AI, Mortimer SE, Yoon T, Painter CA, Jiang W, Mellins ED, Stern LJ
Nature structural & molecular biology20(1)90-8(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results indicate that HLA-DOB is co-inherited with DQA2-B2 genes and thus may contribute to HCV and HBV persistence
Title: Neutralizing Antibody Responses to Viral Infections Are Linked to the Non-classical MHC Class II Gene H2-Ob.

TAP2, HLA-DOA, HLA-DOB, and tapasin loci are novel candidate regions for susceptibility to HCV infection and viral clearance in the Chinese population
Title: Association of polymorphisms in HLA antigen presentation-related genes with the outcomes of HCV infection.

HLA-DO beta-chain antigens are ubiquitinated by MARCH family E3 ligases 1, 8, and 9.
Title: Ubiquitination of HLA-DO by MARCH family E3 ligases.

results show that HLA-DO inhibits HLA-DM function by acting as a substrate mimic, and the findings also limit the possible functional roles for HLA-DO in antigen presentation
Title: HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following HLA-DOB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLA-DOB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19686	NM_002120.4 Latest version!	Homo sapiens major histocompatibility complex, class II, DO beta (HLA-DOB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu19686	NM_002120.3	Homo sapiens major histocompatibility complex, class II, DO beta (HLA-DOB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HLA-DOB ( NM_002120.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002111.1 Homo sapiens major histocompatibility complex, class II, DO beta (HLA-DOB), mRNA.

Price & Availability↑

CloneID	OHu19686
Clone ID Related Accession (Same CDS sequence)	NM_002120.4 , NM_002120.3
Accession Version	NM_002120.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	HLA class II histocompatibility antigen, DO beta chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM152694.1, BC006097.1 and BQ010608.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002120.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006097.1, SRR1163657.226391.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000438763.7/ ENSP00000390020.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGTTCTG GGTGGGTCCC CTGGGTGGTG GCTCTGCTAG TGAATCTGAC CCGACTGGAT 
TCCTCCATGA CTCAAGGCAC AGACTCTCCA GAAGATTTTG TGATTCAGGC AAAGGCTGAC 
TGTTACTTCA CCAACGGGAC AGAAAAGGTG CAGTTTGTGG TCAGATTCAT CTTTAACTTG 
GAGGAGTATG TACGTTTCGA CAGTGATGTG GGGATGTTTG TGGCATTGAC CAAGCTGGGG 
CAGCCAGATG CTGAGCAGTG GAACAGCCGG CTGGATCTCT TGGAGAGGAG CAGACAGGCC 
GTGGATGGGG TCTGTAGACA CAACTACAGG CTGGGCGCAC CCTTCACTGT GGGGAGAAAA 
GTGCAACCAG AGGTGACAGT GTACCCAGAG AGGACCCCAC TCCTGCACCA GCATAATCTG 
CTGCACTGCT CTGTGACAGG CTTCTATCCA GGGGATATCA AGATCAAGTG GTTCCTGAAT 
GGGCAGGAGG AGAGAGCTGG GGTCATGTCC ACTGGCCCTA TCAGGAATGG AGACTGGACC 
TTTCAGACTG TGGTGATGCT AGAAATGACT CCTGAACTTG GACATGTCTA CACCTGCCTT 
GTCGATCACT CCAGCCTGCT GAGCCCTGTT TCTGTGGAGT GGAGAGCTCA GTCTGAATAT 
TCTTGGAGAA AGATGCTGAG TGGCATTGCA GCCTTCCTAC TTGGGCTAAT CTTCCTTCTG 
GTGGGAATCG TCATCCAGCT AAGGGCTCAG AAAGGATATG TGAGGACGCA GATGTCTGGT 
AATGAGGTCT CAAGAGCTGT TCTGCTCCCT CAGTCATGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002111.1
CDS	52..873
Translation	MGSGWVPWVVALLVNLTRLDSSMTQGTDSPEDFVIQAKADCYFTNGTEKVQFVVRFIFNLEEYVRFDSDVGMFVALTKLGQPDAEQWNSRLDLLERSRQAVDGVCRHNYRLGAPFTVGRKVQPEVTVYPERTPLLHQHNLLHCSVTGFYPGDIKIKWFLNGQEERAGVMSTGPIRNGDWTFQTVVMLEMTPELGHVYTCLVDHSSLLSPVSVEWRAQSEYSWRKMLSGIAAFLLGLIFLLVGIVIQLRAQKGYVRTQMSGNEVSRAVLLPQSC

Target ORF information:

RefSeq Version	NM_002120.4
Organism	Homo sapiens(human)
Definition	Homo sapiens major histocompatibility complex, class II, DO beta (HLA-DOB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002120.4

ORF Insert Sequence:

ATGGGTTCTG GGTGGGTCCC CTGGGTGGTG GCTCTGCTAG TGAATCTGAC CCGACTGGAT 
TCCTCCATGA CTCAAGGCAC AGACTCTCCA GAAGATTTTG TGATTCAGGC AAAGGCTGAC 
TGTTACTTCA CCAACGGGAC AGAAAAGGTG CAGTTTGTGG TCAGATTCAT CTTTAACTTG 
GAGGAGTATG TACGTTTCGA CAGTGATGTG GGGATGTTTG TGGCATTGAC CAAGCTGGGG 
CAGCCAGATG CTGAGCAGTG GAACAGCCGG CTGGATCTCT TGGAGAGGAG CAGACAGGCC 
GTGGATGGGG TCTGTAGACA CAACTACAGG CTGGGCGCAC CCTTCACTGT GGGGAGAAAA 
GTGCAACCAG AGGTGACAGT GTACCCAGAG AGGACCCCAC TCCTGCACCA GCATAATCTG 
CTGCACTGCT CTGTGACAGG CTTCTATCCA GGGGATATCA AGATCAAGTG GTTCCTGAAT 
GGGCAGGAGG AGAGAGCTGG GGTCATGTCC ACTGGCCCTA TCAGGAATGG AGACTGGACC 
TTTCAGACTG TGGTGATGCT AGAAATGACT CCTGAACTTG GACATGTCTA CACCTGCCTT 
GTCGATCACT CCAGCCTGCT GAGCCCTGTT TCTGTGGAGT GGAGAGCTCA GTCTGAATAT 
TCTTGGAGAA AGATGCTGAG TGGCATTGCA GCCTTCCTAC TTGGGCTAAT CTTCCTTCTG 
GTGGGAATCG TCATCCAGCT AAGGGCTCAG AAAGGATATG TGAGGACGCA GATGTCTGGT 
AATGAGGTCT CAAGAGCTGT TCTGCTCCCT CAGTCATGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLA-DOB ( NM_002120.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002111.1 Homo sapiens major histocompatibility complex, class II, DO beta (HLA-DOB), mRNA.

Price & Availability↑

CloneID	OHu19686
Clone ID Related Accession (Same CDS sequence)	NM_002120.4 , NM_002120.3
Accession Version	NM_002120.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	HLA class II histocompatibility antigen, DO beta chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM152694.1, BC006097.1 and BQ010608.1. This sequence is a reference standard in the RefSeqGene project. On Nov 24, 2006 this sequence version replaced NM_002120.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006097.1, SRR1163657.226391.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTTCTG GGTGGGTCCC CTGGGTGGTG GCTCTGCTAG TGAATCTGAC CCGACTGGAT 
TCCTCCATGA CTCAAGGCAC AGACTCTCCA GAAGATTTTG TGATTCAGGC AAAGGCTGAC 
TGTTACTTCA CCAACGGGAC AGAAAAGGTG CAGTTTGTGG TCAGATTCAT CTTTAACTTG 
GAGGAGTATG TACGTTTCGA CAGTGATGTG GGGATGTTTG TGGCATTGAC CAAGCTGGGG 
CAGCCAGATG CTGAGCAGTG GAACAGCCGG CTGGATCTCT TGGAGAGGAG CAGACAGGCC 
GTGGATGGGG TCTGTAGACA CAACTACAGG CTGGGCGCAC CCTTCACTGT GGGGAGAAAA 
GTGCAACCAG AGGTGACAGT GTACCCAGAG AGGACCCCAC TCCTGCACCA GCATAATCTG 
CTGCACTGCT CTGTGACAGG CTTCTATCCA GGGGATATCA AGATCAAGTG GTTCCTGAAT 
GGGCAGGAGG AGAGAGCTGG GGTCATGTCC ACTGGCCCTA TCAGGAATGG AGACTGGACC 
TTTCAGACTG TGGTGATGCT AGAAATGACT CCTGAACTTG GACATGTCTA CACCTGCCTT 
GTCGATCACT CCAGCCTGCT GAGCCCTGTT TCTGTGGAGT GGAGAGCTCA GTCTGAATAT 
TCTTGGAGAA AGATGCTGAG TGGCATTGCA GCCTTCCTAC TTGGGCTAAT CTTCCTTCTG 
GTGGGAATCG TCATCCAGCT AAGGGCTCAG AAAGGATATG TGAGGACGCA GATGTCTGGT 
AATGAGGTCT CAAGAGCTGT TCTGCTCCCT CAGTCATGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002111.1
CDS	98..919
Translation	MGSGWVPWVVALLVNLTRLDSSMTQGTDSPEDFVIQAKADCYFTNGTEKVQFVVRFIFNLEEYVRFDSDVGMFVALTKLGQPDAEQWNSRLDLLERSRQAVDGVCRHNYRLGAPFTVGRKVQPEVTVYPERTPLLHQHNLLHCSVTGFYPGDIKIKWFLNGQEERAGVMSTGPIRNGDWTFQTVVMLEMTPELGHVYTCLVDHSSLLSPVSVEWRAQSEYSWRKMLSGIAAFLLGLIFLLVGIVIQLRAQKGYVRTQMSGNEVSRAVLLPQSC

Target ORF information:

RefSeq Version	NM_002120.3
Organism	Homo sapiens(human)
Definition	Homo sapiens major histocompatibility complex, class II, DO beta (HLA-DOB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002120.3

ORF Insert Sequence:

ATGGGTTCTG GGTGGGTCCC CTGGGTGGTG GCTCTGCTAG TGAATCTGAC CCGACTGGAT 
TCCTCCATGA CTCAAGGCAC AGACTCTCCA GAAGATTTTG TGATTCAGGC AAAGGCTGAC 
TGTTACTTCA CCAACGGGAC AGAAAAGGTG CAGTTTGTGG TCAGATTCAT CTTTAACTTG 
GAGGAGTATG TACGTTTCGA CAGTGATGTG GGGATGTTTG TGGCATTGAC CAAGCTGGGG 
CAGCCAGATG CTGAGCAGTG GAACAGCCGG CTGGATCTCT TGGAGAGGAG CAGACAGGCC 
GTGGATGGGG TCTGTAGACA CAACTACAGG CTGGGCGCAC CCTTCACTGT GGGGAGAAAA 
GTGCAACCAG AGGTGACAGT GTACCCAGAG AGGACCCCAC TCCTGCACCA GCATAATCTG 
CTGCACTGCT CTGTGACAGG CTTCTATCCA GGGGATATCA AGATCAAGTG GTTCCTGAAT 
GGGCAGGAGG AGAGAGCTGG GGTCATGTCC ACTGGCCCTA TCAGGAATGG AGACTGGACC 
TTTCAGACTG TGGTGATGCT AGAAATGACT CCTGAACTTG GACATGTCTA CACCTGCCTT 
GTCGATCACT CCAGCCTGCT GAGCCCTGTT TCTGTGGAGT GGAGAGCTCA GTCTGAATAT 
TCTTGGAGAA AGATGCTGAG TGGCATTGCA GCCTTCCTAC TTGGGCTAAT CTTCCTTCTG 
GTGGGAATCG TCATCCAGCT AAGGGCTCAG AAAGGATATG TGAGGACGCA GATGTCTGGT 
AATGAGGTCT CAAGAGCTGT TCTGCTCCCT CAGTCATGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neutralizing Antibody Responses to Viral Infections Are Linked to the Non-classical MHC Class II Gene H2-Ob.
Immunity47(2)310-322.e7(2017 Aug)
Denzin LK,Khan AA,Virdis F,Wilks J,Kane M,Beilinson HA,Dikiy S,Case LK,Roopenian D,Witkowski M,Chervonsky AV,Golovkina TV

Association of polymorphisms in HLA antigen presentation-related genes with the outcomes of HCV infection.
PloS one10(4)e0123513(2015)
Huang P,Zhang Y,Lu X,Xu Y,Wang J,Zhang Y,Yu R,Su J

Ubiquitination of HLA-DO by MARCH family E3 ligases.
European journal of immunology43(5)1153-61(2013 May)
Jahnke M,Trowsdale J,Kelly AP

A genome-wide association study identifies 2 susceptibility Loci for Crohn's disease in a Japanese population.
Gastroenterology144(4)781-8(2013 Apr)
Yamazaki K,Umeno J,Takahashi A,Hirano A,Johnson TA,Kumasaka N,Morizono T,Hosono N,Kawaguchi T,Takazoe M,Yamada T,Suzuki Y,Tanaka H,Motoya S,Hosokawa M,Arimura Y,Shinomura Y,Matsui T,Matsumoto T,Iida M,Tsunoda T,Nakamura Y,Kamatani N,Kubo M

HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism.
Nature structural & molecular biology20(1)90-8(2013 Jan)
Guce AI,Mortimer SE,Yoon T,Painter CA,Jiang W,Mellins ED,Stern LJ

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HLA-DOB cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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