HLA-DMB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HLA-DMB
Entrez Gene ID	3109
Full Name	major histocompatibility complex, class II, DM beta
Synonyms	D6S221E,RING7
General protein information	Preferred Names major histocompatibility complex, class II, DM beta Names HLA class II histocompatibility antigen, DM beta chain MHC class II HLA-DMB MHC class II antigen DMB MHC class II antigen HLA-DM beta chain class II histocompatibility antigen, M beta chain really interesting new gene 7 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.32
Summary	HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008].
Disorder MIM:	142856

mRNA and Protein(s)

mRNA	Protein	Name
NM_002118.5	NP_002109.2	HLA class II histocompatibility antigen, DM beta chain precursor
NM_002118.4	NP_002109.2	HLA class II histocompatibility antigen, DM beta chain precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	BOLA-DMB	NP_001035571.1
Pan troglodytes (chimpanzee)	PATR-DMB	NP_001127814.1
Mus musculus (house mouse)	H2-DMb1	NP_034517.2
Homo sapiens (human)	HLA-DMB	NP_002109.2
Canis lupus familiaris (dog)	DLA-DMB	NP_001257999.1
Gallus gallus (chicken)	BMA2	NP_001128638.1
Xenopus tropicalis (tropical clawed frog)	LOC100495183	XP_002942059.1
	MAMU-DMB	XP_002803734.1
Mus musculus (house mouse)	H2-DMb2	NP_034518.1

Gene Ontology
Bibliography

Related articles in PubMed

Evaluating the Role of HLA-DM in MHC Class II-Peptide Association Reactions.
Yin L, Maben ZJ, Becerra A, Stern LJ
Journal of immunology (Baltimore, Md. : 1950)195(2)706-16(2015 Jul)

SNP screening of central MHC-identified HLA-DMB as a candidate susceptibility gene for HIV-related Kaposi's sarcoma.
Aissani B, Boehme AK, Wiener HW, Shrestha S, Jacobson LP, Kaslow RA
Genes and immunity15(6)424-9(2014 Sep)

HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism.
Guce AI, Mortimer SE, Yoon T, Painter CA, Jiang W, Mellins ED, Stern LJ
Nature structural & molecular biology20(1)90-8(2013 Jan)

Crystal structure of the HLA-DM-HLA-DR1 complex defines mechanisms for rapid peptide selection.
Pos W, Sethi DK, Call MJ, Schulze MS, Anders AK, Pyrdol J, Wucherpfennig KW
Cell151(7)1557-68(2012 Dec)

Mapping the HLA-DO/HLA-DM complex by FRET and mutagenesis.
Yoon T, Macmillan H, Mortimer SE, Jiang W, Rinderknecht CH, Stern LJ, Mellins ED
Proceedings of the National Academy of Sciences of the United States of America109(28)11276-81(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study provides novel insights into how HLA-DM efficiently catalyzes peptide loading during antigen presentation.
Title: Evaluating the Role of HLA-DM in MHC Class II-Peptide Association Reactions.

Data suggest that antigen processing by MHC class II HLA-DMB antigen is a target pathway in the pathogenesis of HIV-related Kaposi's sarcoma.
Title: SNP screening of central MHC-identified HLA-DMB as a candidate susceptibility gene for HIV-related Kaposi's sarcoma.

The structure of the HLA-DM-HLA-DR complex shows major rearrangements of the HLA-DR peptide-binding groove.
Title: Crystal structure of the HLA-DM-HLA-DR1 complex defines mechanisms for rapid peptide selection.

DMA*01:01 (69.35%) and DMB*01:01 (52.5%) alleles were more frequent in Chinese Hans
Title: Analysis of HLA-DM polymorphisms in the Chinese Han population.

Overexpression of HLA-DM at the plasma membrane of dendritic cells improves quantitatively, but also qualitatively, the presentation of CD4-expressing T cell epitopes in cellular vaccine therapies for cancer.
Title: Forced expression of HLA-DM at the surface of dendritic cells increases loading of synthetic peptides on MHC class II molecules and modulates T cell responses.

The following HLA-DMB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLA-DMB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03841	NM_002118.5 Latest version!	Homo sapiens major histocompatibility complex, class II, DM beta (HLA-DMB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu03841	NM_002118.4	Homo sapiens major histocompatibility complex, class II, DM beta (HLA-DMB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HLA-DMB ( NM_002118.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002109.2 Homo sapiens major histocompatibility complex, class II, DM beta (HLA-DMB), mRNA.

Price & Availability↑

CloneID	OHu03841
Clone ID Related Accession (Same CDS sequence)	NM_002118.5 , NM_002118.4
Accession Version	NM_002118.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	HLA class II histocompatibility antigen, DM beta chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U15085.1, EL948395.1 and CD742571.1. On Nov 22, 2018 this sequence version replaced NM_002118.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U15085.1, AY645722.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000418107.3/ ENSP00000398890.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATCACAT TCCTGCCGCT GCTGCTGGGG CTCAGCCTGG GCTGCACAGG AGCAGGTGGC 
TTCGTGGCCC ATGTGGAAAG CACCTGTCTG TTGGATGATG CTGGGACTCC AAAGGATTTC 
ACATACTGCA TCTCCTTCAA CAAGGATCTG CTGACCTGCT GGGATCCAGA GGAGAATAAG 
ATGGCCCCTT GCGAATTTGG GGTGCTGAAT AGCTTGGCGA ATGTCCTCTC ACAGCACCTC 
AACCAAAAAG ACACCCTGAT GCAGCGCTTG CGCAATGGGC TTCAGAATTG TGCCACACAC 
ACCCAGCCCT TCTGGGGATC ACTGACCAAC AGGACACGGC CACCATCTGT GCAAGTAGCC 
AAAACCACTC CTTTTAACAC GAGGGAGCCT GTGATGCTGG CCTGCTATGT GTGGGGCTTC 
TATCCAGCAG AAGTGACTAT CACGTGGAGG AAGAACGGGA AGCTTGTCAT GCCTCACAGC 
AGTGCGCACA AGACTGCCCA GCCCAATGGA GACTGGACAT ACCAGACCCT CTCCCATTTA 
GCCTTAACCC CCTCTTACGG GGACACTTAC ACCTGTGTGG TAGAGCACAC TGGGGCTCCT 
GAGCCCATCC TTCGGGACTG GACACCTGGG CTGTCCCCCA TGCAGACCCT GAAGGTTTCT 
GTGTCTGCAG TGACTCTGGG CCTGGGCCTC ATCATCTTCT CTCTTGGTGT GATCAGCTGG 
CGGAGAGCTG GCCACTCTAG TTACACTCCT CTTCCTGGGT CCAATTATTC AGAAGGATGG 
CACATTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002109.2
CDS	222..1013
Translation	MITFLPLLLGLSLGCTGAGGFVAHVESTCLLDDAGTPKDFTYCISFNKDLLTCWDPEENKMAPCEFGVLNSLANVLSQHLNQKDTLMQRLRNGLQNCATHTQPFWGSLTNRTRPPSVQVAKTTPFNTREPVMLACYVWGFYPAEVTITWRKNGKLVMPHSSAHKTAQPNGDWTYQTLSHLALTPSYGDTYTCVVEHTGAPEPILRDWTPGLSPMQTLKVSVSAVTLGLGLIIFSLGVISWRRAGHSSYTPLPGSNYSEGWHIS

Target ORF information:

RefSeq Version	NM_002118.5
Organism	Homo sapiens(human)
Definition	Homo sapiens major histocompatibility complex, class II, DM beta (HLA-DMB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002118.5

ORF Insert Sequence:

ATGATCACAT TCCTGCCGCT GCTGCTGGGG CTCAGCCTGG GCTGCACAGG AGCAGGTGGC 
TTCGTGGCCC ATGTGGAAAG CACCTGTCTG TTGGATGATG CTGGGACTCC AAAGGATTTC 
ACATACTGCA TCTCCTTCAA CAAGGATCTG CTGACCTGCT GGGATCCAGA GGAGAATAAG 
ATGGCCCCTT GCGAATTTGG GGTGCTGAAT AGCTTGGCGA ATGTCCTCTC ACAGCACCTC 
AACCAAAAAG ACACCCTGAT GCAGCGCTTG CGCAATGGGC TTCAGAATTG TGCCACACAC 
ACCCAGCCCT TCTGGGGATC ACTGACCAAC AGGACACGGC CACCATCTGT GCAAGTAGCC 
AAAACCACTC CTTTTAACAC GAGGGAGCCT GTGATGCTGG CCTGCTATGT GTGGGGCTTC 
TATCCAGCAG AAGTGACTAT CACGTGGAGG AAGAACGGGA AGCTTGTCAT GCCTCACAGC 
AGTGCGCACA AGACTGCCCA GCCCAATGGA GACTGGACAT ACCAGACCCT CTCCCATTTA 
GCCTTAACCC CCTCTTACGG GGACACTTAC ACCTGTGTGG TAGAGCACAC TGGGGCTCCT 
GAGCCCATCC TTCGGGACTG GACACCTGGG CTGTCCCCCA TGCAGACCCT GAAGGTTTCT 
GTGTCTGCAG TGACTCTGGG CCTGGGCCTC ATCATCTTCT CTCTTGGTGT GATCAGCTGG 
CGGAGAGCTG GCCACTCTAG TTACACTCCT CTTCCTGGGT CCAATTATTC AGAAGGATGG 
CACATTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLA-DMB ( NM_002118.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002109.2 Homo sapiens major histocompatibility complex, class II, DM beta (HLA-DMB), mRNA.

Price & Availability↑

CloneID	OHu03841
Clone ID Related Accession (Same CDS sequence)	NM_002118.5 , NM_002118.4
Accession Version	NM_002118.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	HLA class II histocompatibility antigen, DM beta chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC389968.1, U15085.1, EL948395.1 and CD742571.1. On Jul 23, 2010 this sequence version replaced NM_002118.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U15085.1, AY645722.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCACAT TCCTGCCGCT GCTGCTGGGG CTCAGCCTGG GCTGCACAGG AGCAGGTGGC 
TTCGTGGCCC ATGTGGAAAG CACCTGTCTG TTGGATGATG CTGGGACTCC AAAGGATTTC 
ACATACTGCA TCTCCTTCAA CAAGGATCTG CTGACCTGCT GGGATCCAGA GGAGAATAAG 
ATGGCCCCTT GCGAATTTGG GGTGCTGAAT AGCTTGGCGA ATGTCCTCTC ACAGCACCTC 
AACCAAAAAG ACACCCTGAT GCAGCGCTTG CGCAATGGGC TTCAGAATTG TGCCACACAC 
ACCCAGCCCT TCTGGGGATC ACTGACCAAC AGGACACGGC CACCATCTGT GCAAGTAGCC 
AAAACCACTC CTTTTAACAC GAGGGAGCCT GTGATGCTGG CCTGCTATGT GTGGGGCTTC 
TATCCAGCAG AAGTGACTAT CACGTGGAGG AAGAACGGGA AGCTTGTCAT GCCTCACAGC 
AGTGCGCACA AGACTGCCCA GCCCAATGGA GACTGGACAT ACCAGACCCT CTCCCATTTA 
GCCTTAACCC CCTCTTACGG GGACACTTAC ACCTGTGTGG TAGAGCACAC TGGGGCTCCT 
GAGCCCATCC TTCGGGACTG GACACCTGGG CTGTCCCCCA TGCAGACCCT GAAGGTTTCT 
GTGTCTGCAG TGACTCTGGG CCTGGGCCTC ATCATCTTCT CTCTTGGTGT GATCAGCTGG 
CGGAGAGCTG GCCACTCTAG TTACACTCCT CTTCCTGGGT CCAATTATTC AGAAGGATGG 
CACATTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002109.2
CDS	264..1055
Translation	MITFLPLLLGLSLGCTGAGGFVAHVESTCLLDDAGTPKDFTYCISFNKDLLTCWDPEENKMAPCEFGVLNSLANVLSQHLNQKDTLMQRLRNGLQNCATHTQPFWGSLTNRTRPPSVQVAKTTPFNTREPVMLACYVWGFYPAEVTITWRKNGKLVMPHSSAHKTAQPNGDWTYQTLSHLALTPSYGDTYTCVVEHTGAPEPILRDWTPGLSPMQTLKVSVSAVTLGLGLIIFSLGVISWRRAGHSSYTPLPGSNYSEGWHIS

Target ORF information:

RefSeq Version	NM_002118.4
Organism	Homo sapiens(human)
Definition	Homo sapiens major histocompatibility complex, class II, DM beta (HLA-DMB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002118.4

ORF Insert Sequence:

ATGATCACAT TCCTGCCGCT GCTGCTGGGG CTCAGCCTGG GCTGCACAGG AGCAGGTGGC 
TTCGTGGCCC ATGTGGAAAG CACCTGTCTG TTGGATGATG CTGGGACTCC AAAGGATTTC 
ACATACTGCA TCTCCTTCAA CAAGGATCTG CTGACCTGCT GGGATCCAGA GGAGAATAAG 
ATGGCCCCTT GCGAATTTGG GGTGCTGAAT AGCTTGGCGA ATGTCCTCTC ACAGCACCTC 
AACCAAAAAG ACACCCTGAT GCAGCGCTTG CGCAATGGGC TTCAGAATTG TGCCACACAC 
ACCCAGCCCT TCTGGGGATC ACTGACCAAC AGGACACGGC CACCATCTGT GCAAGTAGCC 
AAAACCACTC CTTTTAACAC GAGGGAGCCT GTGATGCTGG CCTGCTATGT GTGGGGCTTC 
TATCCAGCAG AAGTGACTAT CACGTGGAGG AAGAACGGGA AGCTTGTCAT GCCTCACAGC 
AGTGCGCACA AGACTGCCCA GCCCAATGGA GACTGGACAT ACCAGACCCT CTCCCATTTA 
GCCTTAACCC CCTCTTACGG GGACACTTAC ACCTGTGTGG TAGAGCACAC TGGGGCTCCT 
GAGCCCATCC TTCGGGACTG GACACCTGGG CTGTCCCCCA TGCAGACCCT GAAGGTTTCT 
GTGTCTGCAG TGACTCTGGG CCTGGGCCTC ATCATCTTCT CTCTTGGTGT GATCAGCTGG 
CGGAGAGCTG GCCACTCTAG TTACACTCCT CTTCCTGGGT CCAATTATTC AGAAGGATGG 
CACATTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evaluating the Role of HLA-DM in MHC Class II-Peptide Association Reactions.
Journal of immunology (Baltimore, Md. : 1950)195(2)706-16(2015 Jul)
Yin L,Maben ZJ,Becerra A,Stern LJ

SNP screening of central MHC-identified HLA-DMB as a candidate susceptibility gene for HIV-related Kaposi's sarcoma.
Genes and immunity15(6)424-9(2014 Sep)
Aissani B,Boehme AK,Wiener HW,Shrestha S,Jacobson LP,Kaslow RA

HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism.
Nature structural & molecular biology20(1)90-8(2013 Jan)
Guce AI,Mortimer SE,Yoon T,Painter CA,Jiang W,Mellins ED,Stern LJ

Crystal structure of the HLA-DM-HLA-DR1 complex defines mechanisms for rapid peptide selection.
Cell151(7)1557-68(2012 Dec)
Pos W,Sethi DK,Call MJ,Schulze MS,Anders AK,Pyrdol J,Wucherpfennig KW

Mapping the HLA-DO/HLA-DM complex by FRET and mutagenesis.
Proceedings of the National Academy of Sciences of the United States of America109(28)11276-81(2012 Jul)
Yoon T,Macmillan H,Mortimer SE,Jiang W,Rinderknecht CH,Stern LJ,Mellins ED

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HLA-DMB cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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