HINT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HINT1
Entrez Gene ID	3094
Full Name	histidine triad nucleotide binding protein 1
Synonyms	HINT,NMAN,PKCI-1,PRKCNH1
General protein information	Preferred Names histidine triad nucleotide binding protein 1 Names histidine triad nucleotide-binding protein 1 adenosine 5'-monophosphoramidase protein kinase C inhibitor 1 protein kinase C-interacting protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q23.3
Summary	This gene encodes a protein that hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif. This gene is considered a tumor suppressor gene. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed. [provided by RefSeq, Dec 2015].
Disorder MIM:	601314
Disorder Html:	Neuromyotonia and axonal neuropathy, autosomal recessive, 137200 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_005340.7	NP_005331.1	histidine triad nucleotide-binding protein 1
XM_017009410.1	XP_016864899.1	histidine triad nucleotide-binding protein 1 isoform X1
NM_005340.6	NP_005331.1	histidine triad nucleotide-binding protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HINT1	XP_003310848.1
Drosophila melanogaster (fruit fly)	CG2862	NP_608711.3
Xenopus tropicalis (tropical clawed frog)	hint1	NP_001016165.1
Bos taurus (cattle)	HINT1	NP_787006.1
Danio rerio (zebrafish)	hint1	NP_001005593.1
	HINT1	XP_001099859.1
Mus musculus (house mouse)	Hint1	NP_032274.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006353	XP_316373.4
Caenorhabditis elegans (roundworm)	hint-1	NP_492056.1
Arabidopsis thaliana (thale cress)	HIT3	NP_567038.1
Oryza sativa (rice)	Os03g0322500	NP_001049967.1
Canis lupus familiaris (dog)	HINT1	XP_531895.2
Gallus gallus (chicken)	HINT1	NP_990020.1
Homo sapiens (human)	HINT1	NP_005331.1
Rattus norvegicus (Norway rat)	Hint1	NP_001103077.1

Gene Ontology
Bibliography

Related articles in PubMed

Emerging Roles of Histidine Triad Nucleotide Binding Protein 1 in Neuropsychiatric Diseases.
Dang YH, Liu ZW, Liu P, Wang JB
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae39(5)705-714(2017 Oct)

Inhibition by divalent metal ions of human histidine triad nucleotide binding protein1 (hHint1), a regulator of opioid analgesia and neuropathic pain.
Shah R, Chou TF, Maize KM, Strom A, Finzel BC, Wagner CR
Biochemical and biophysical research communications491(3)760-766(2017 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thus, hHint1 appears to be structurally sensitive to irreversible inactivation by copper, which may be of neurotoxicological and pharmacological significance.
Title: Inhibition by divalent metal ions of human histidine triad nucleotide binding protein1 (hHint1), a regulator of opioid analgesia and neuropathic pain.

Hint1 has been shown to be essential for the metabolic activation of nucleotide antiviral pronucleotides (i.e., proTides). These results provide mechanistic insights underpinning histidine nucleophilic catalysis in general and human Hint1 catalysis, in particular, thus aiding the design of future proTides and the elucidation of the natural function of the Hint family of enzymes.
Title: Caught before Released: Structural Mapping of the Reaction Trajectory for the Sofosbuvir Activating Enzyme, Human Histidine Triad Nucleotide Binding Protein 1 (hHint1).

We provide a comprehensive overview of the structural and functional characteristics of the HINT1 protein that may guide further studies into the molecular aetiology and treatment strategies--{REVIEW}
Title: Axonal neuropathy with neuromyotonia: there is a HINT.

Underexpression of Hint1 is associated with invasion of hepatocellular carcinoma.
Title: Hint1 suppresses migration and invasion of hepatocellular carcinoma cells in vitro by modulating girdin activity.

first structure (at a 2.34A resolution) of a complex of human HINT1 with a non-hydrolyzable analog of an Ap4A dinucleotide
Title: Crystallographic studies of the complex of human HINT1 protein with a non-hydrolyzable analog of Ap4A.

The following HINT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HINT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27559	NM_005340.7 Latest version!	Homo sapiens histidine triad nucleotide binding protein 1 (HINT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu58212	XM_017009410.1 Latest version!	Homo sapiens histidine triad nucleotide binding protein 1 (HINT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu27559	NM_005340.6	Homo sapiens histidine triad nucleotide binding protein 1 (HINT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HINT1 ( NM_005340.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_005331.1 Homo sapiens histidine triad nucleotide binding protein 1 (HINT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27559
Clone ID Related Accession (Same CDS sequence)	NM_005340.7 , NM_005340.6
Accession Version	NM_005340.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	histidine triad nucleotide-binding protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC007090.1, AA076103.1 and AC004650.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_005340.6. Transcript Variant: This variant (1) encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.404584.1, SRR1163658.189956.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000304043.10/ ENSP00000304229.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGATG AGATTGCCAA GGCTCAGGTC GCTCGGCCTG GTGGCGACAC GATCTTTGGG 
AAGATCATCC GCAAGGAAAT ACCAGCCAAA ATCATTTTTG AGGATGACCG GTGCCTTGCT 
TTCCATGACA TTTCCCCTCA AGCACCAACA CATTTTCTGG TGATACCCAA GAAACATATA 
TCCCAGATTT CTGTGGCAGA AGATGATGAT GAAAGTCTTC TTGGACACTT AATGATTGTT 
GGCAAGAAAT GTGCTGCTGA TCTGGGCCTG AATAAGGGTT ATCGAATGGT GGTGAATGAA 
GGTTCAGATG GTGGACAGTC TGTCTATCAC GTTCATCTCC ATGTTCTTGG AGGTCGGCAA 
ATGCATTGGC CTCCTGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005331.1
CDS	52..432
Translation	MADEIAKAQVARPGGDTIFGKIIRKEIPAKIIFEDDRCLAFHDISPQAPTHFLVIPKKHISQISVAEDDDESLLGHLMIVGKKCAADLGLNKGYRMVVNEGSDGGQSVYHVHLHVLGGRQMHWPPG

Target ORF information:

RefSeq Version	NM_005340.7
Organism	Homo sapiens(human)
Definition	Homo sapiens histidine triad nucleotide binding protein 1 (HINT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005340.7

ORF Insert Sequence:

ATGGCAGATG AGATTGCCAA GGCTCAGGTC GCTCGGCCTG GTGGCGACAC GATCTTTGGG 
AAGATCATCC GCAAGGAAAT ACCAGCCAAA ATCATTTTTG AGGATGACCG GTGCCTTGCT 
TTCCATGACA TTTCCCCTCA AGCACCAACA CATTTTCTGG TGATACCCAA GAAACATATA 
TCCCAGATTT CTGTGGCAGA AGATGATGAT GAAAGTCTTC TTGGACACTT AATGATTGTT 
GGCAAGAAAT GTGCTGCTGA TCTGGGCCTG AATAAGGGTT ATCGAATGGT GGTGAATGAA 
GGTTCAGATG GTGGACAGTC TGTCTATCAC GTTCATCTCC ATGTTCTTGG AGGTCGGCAA 
ATGCATTGGC CTCCTGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HINT1 ( NM_005340.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_005331.1 Homo sapiens histidine triad nucleotide binding protein 1 (HINT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27559
Clone ID Related Accession (Same CDS sequence)	NM_005340.7 , NM_005340.6
Accession Version	NM_005340.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	histidine triad nucleotide-binding protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF967059.1, BC007090.1 and AA076103.1. This sequence is a reference standard in the RefSeqGene project. On Dec 5, 2012 this sequence version replaced NM_005340.5. Transcript Variant: This variant (1) encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.268590.1, SRR1803612.174117.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGATG AGATTGCCAA GGCTCAGGTC GCTCGGCCTG GTGGCGACAC GATCTTTGGG 
AAGATCATCC GCAAGGAAAT ACCAGCCAAA ATCATTTTTG AGGATGACCG GTGCCTTGCT 
TTCCATGACA TTTCCCCTCA AGCACCAACA CATTTTCTGG TGATACCCAA GAAACATATA 
TCCCAGATTT CTGTGGCAGA AGATGATGAT GAAAGTCTTC TTGGACACTT AATGATTGTT 
GGCAAGAAAT GTGCTGCTGA TCTGGGCCTG AATAAGGGTT ATCGAATGGT GGTGAATGAA 
GGTTCAGATG GTGGACAGTC TGTCTATCAC GTTCATCTCC ATGTTCTTGG AGGTCGGCAA 
ATGCATTGGC CTCCTGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005331.1
CDS	144..524
Translation	MADEIAKAQVARPGGDTIFGKIIRKEIPAKIIFEDDRCLAFHDISPQAPTHFLVIPKKHISQISVAEDDDESLLGHLMIVGKKCAADLGLNKGYRMVVNEGSDGGQSVYHVHLHVLGGRQMHWPPG

Target ORF information:

RefSeq Version	NM_005340.6
Organism	Homo sapiens(human)
Definition	Homo sapiens histidine triad nucleotide binding protein 1 (HINT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005340.6

ORF Insert Sequence:

ATGGCAGATG AGATTGCCAA GGCTCAGGTC GCTCGGCCTG GTGGCGACAC GATCTTTGGG 
AAGATCATCC GCAAGGAAAT ACCAGCCAAA ATCATTTTTG AGGATGACCG GTGCCTTGCT 
TTCCATGACA TTTCCCCTCA AGCACCAACA CATTTTCTGG TGATACCCAA GAAACATATA 
TCCCAGATTT CTGTGGCAGA AGATGATGAT GAAAGTCTTC TTGGACACTT AATGATTGTT 
GGCAAGAAAT GTGCTGCTGA TCTGGGCCTG AATAAGGGTT ATCGAATGGT GGTGAATGAA 
GGTTCAGATG GTGGACAGTC TGTCTATCAC GTTCATCTCC ATGTTCTTGG AGGTCGGCAA 
ATGCATTGGC CTCCTGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HINT1 ( XM_017009410.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864899.1 Homo sapiens histidine triad nucleotide binding protein 1 (HINT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu58212
Clone ID Related Accession (Same CDS sequence)	XM_017009410.1
Accession Version	XM_017009410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	histidine triad nucleotide-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG AGATTGCCAA GGCTCAGGTC GCTCGGCCTG GTGGCGACAC GATCTTTGGG 
AAGATCATCC GCAAGGAAAT ACCAGCCAAA ATCATTTTTG AGGATGACCG GTGCCTTGCT 
TTCCATGACA TTTCCCCTCA AGCACCAACA CATTTTCTGG TGATACCCAA GAAACATATA 
TCCCAGATTT CTGTGGCAGA AGATGATGAT GAAAGTGTCA TTACTAAGGA AAAACCAGAA 
AAACCATTAG GACTTCAACT GCCCAGCTGT TTTCCAAAGT TACTGCATCA TTTTGTATCC 
CACCAGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864899.1
CDS	137..448
Translation	MADEIAKAQVARPGGDTIFGKIIRKEIPAKIIFEDDRCLAFHDISPQAPTHFLVIPKKHISQISVAEDDDESVITKEKPEKPLGLQLPSCFPKLLHHFVSHQQ

Target ORF information:

RefSeq Version	XM_017009410.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidine triad nucleotide binding protein 1 (HINT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009410.1

ORF Insert Sequence:

ATGGCAGATG AGATTGCCAA GGCTCAGGTC GCTCGGCCTG GTGGCGACAC GATCTTTGGG 
AAGATCATCC GCAAGGAAAT ACCAGCCAAA ATCATTTTTG AGGATGACCG GTGCCTTGCT 
TTCCATGACA TTTCCCCTCA AGCACCAACA CATTTTCTGG TGATACCCAA GAAACATATA 
TCCCAGATTT CTGTGGCAGA AGATGATGAT GAAAGTGTCA TTACTAAGGA AAAACCAGAA 
AAACCATTAG GACTTCAACT GCCCAGCTGT TTTCCAAAGT TACTGCATCA TTTTGTATCC 
CACCAGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Emerging Roles of Histidine Triad Nucleotide Binding Protein 1 in Neuropsychiatric Diseases.
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae39(5)705-714(2017 Oct)
Dang YH,Liu ZW,Liu P,Wang JB

Inhibition by divalent metal ions of human histidine triad nucleotide binding protein1 (hHint1), a regulator of opioid analgesia and neuropathic pain.
Biochemical and biophysical research communications491(3)760-766(2017 09)
Shah R,Chou TF,Maize KM,Strom A,Finzel BC,Wagner CR

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HINT1 cDNA ORF clone, Homo sapiens(human)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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