EHD3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EHD3
Entrez Gene ID	30845
Full Name	EH domain containing 3
Synonyms	PAST3
General protein information	Preferred Names EH domain containing 3 Names EH domain-containing protein 3 PAST homolog 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p23.1
Disorder MIM:	605891

mRNA and Protein(s)

mRNA	Protein	Name
NM_014600.3	NP_055415.1	EH domain-containing protein 3
NM_014600.2	NP_055415.1	EH domain-containing protein 3
XM_011532806.2	XP_011531108.1	EH domain-containing protein 3 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ehd3	NP_065603.2
Arabidopsis thaliana (thale cress)	EHD2	NP_974517.1
Homo sapiens (human)	EHD3	NP_055415.1
Pan troglodytes (chimpanzee)	EHD3	XP_515386.2
Oryza sativa (rice)	Os06g0687800	NP_001058410.1
	EHD3	XP_001105262.1
Canis lupus familiaris (dog)	EHD3	XP_853995.2
Bos taurus (cattle)	EHD3	NP_001091473.1
Rattus norvegicus (Norway rat)	Ehd3	NP_620245.2
Oryza sativa (rice)	Os02g0158100	NP_001045954.1
Gallus gallus (chicken)	EHD3	NP_001026211.1
Danio rerio (zebrafish)	ehd3	NP_001038469.1

Gene Ontology
Bibliography

Related articles in PubMed

Spatio-temporal regulation of EGFR signaling by the Eps15 homology domain-containing protein 3 (EHD3).
Amessou M, Ebrahim AS, Dilly A, Joseph M, Tabolina M, Chukkapalli S, Meroueh L, Syed JT, Liddane A, Lang SL, Al-Katib A, Kandouz M
Oncotarget7(48)79203-79216(2016 Nov)

EHD3 Protein Is Required for Tubular Recycling Endosome Stabilization, and an Asparagine-Glutamic Acid Residue Pair within Its Eps15 Homology (EH) Domain Dictates Its Selective Binding to NPF Peptides.
Bahl K, Xie S, Spagnol G, Sorgen P, Naslavsky N, Caplan S
The Journal of biological chemistry291(26)13465-78(2016 Jun)

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.
Lu Q, Insinna C, Ott C, Stauffer J, Pintado PA, Rahajeng J, Baxa U, Walia V, Cuenca A, Hwang YS, Daar IO, Lopes S, Lippincott-Schwartz J, Jackson PK, Caplan S, Westlake CJ
Nature cell biology17(3)228-240(2015 Mar)

SUMOylation of EHD3 Modulates Tubulation of the Endocytic Recycling Compartment.
Cabasso O, Pekar O, Horowitz M
PloS one10(7)e0134053(2015)

Association between EHD3 gene and the cognitive function of patients with major depressive disorder.
Wang L, Shi CJ, Xu Q
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae36(3)227-33(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the residues Ala-519/Asp-520 of EHD1 and Asn-519/Glu-520 of EHD3 as defining the selectivity of these two paralogs for NPF-containing binding partners
Title: EHD3 Protein Is Required for Tubular Recycling Endosome Stabilization, and an Asparagine-Glutamic Acid Residue Pair within Its Eps15 Homology (EH) Domain Dictates Its Selective Binding to NPF Peptides.

The EHD3 gene may be associated with the endophenotype of cognitive function in major depressive disorder patients.
Title: Association between EHD3 gene and the cognitive function of patients with major depressive disorder.

3 SNPs present in the EHD3 gene, of which rs619002 and rs644926 were exclusively associated with female major depressive disorder
Title: The gender-specific association of EHD3 polymorphisms with major depressive disorder.

demonstrate Ehd3 growth inhibitory functions in glioma cells in vivo. We suggest that Ehd3 functions as a tumor suppressor gene and loss of its expression is a very common event in gliomas
Title: Ehd3, a regulator of vesicular trafficking, is silenced in gliomas and functions as a tumor suppressor by controlling cell cycle arrest and apoptosis.

evidence that the functions of both EHD1 and EHD4 are primarily in TRE membrane vesiculation, whereas EHD3 is a membrane-tubulating protein.
Title: Differential roles of C-terminal Eps15 homology domain proteins as vesiculators and tubulators of recycling endosomes.

The following EHD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EHD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13901	NM_014600.3 Latest version!	Homo sapiens EH domain containing 3 (EHD3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$314.30 $449.00
OHu67488	XM_011532806.2 Latest version!	Homo sapiens EH domain containing 3 (EHD3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu13901	NM_014600.2	Homo sapiens EH domain containing 3 (EHD3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$314.30 $449.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EHD3 ( NM_014600.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055415.1 Homo sapiens EH domain containing 3 (EHD3), mRNA.

Price & Availability↑

CloneID	OHu13901
Clone ID Related Accession (Same CDS sequence)	NM_014600.3 , NM_014600.2
Accession Version	NM_014600.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	EH domain-containing protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK316006.1, DA196836.1, BC033100.1, AF214736.1 and AL121657.4. On Nov 23, 2018 this sequence version replaced NM_014600.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.27274.1, SRR1803615.35683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000322054.10/ ENSP00000327116.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTCAGCT GGCTGGGTAC GGACGACCGC CGGAGGAAGG ACCCCGAGGT TTTCCAGACG 
GTGAGTGAGG GGCTCAAGAA ACTCTACAAG AGCAAGCTGC TGCCCTTGGA AGAGCATTAC 
CGCTTCCACG AGTTCCACTC GCCCGCCCTG GAGGATGCCG ACTTCGACAA CAAGCCCATG 
GTTCTGCTGG TGGGCCAGTA CTCCACTGGG AAGACCACCT TCATCAGGTA CCTGCTGGAA 
CAGGACTTCC CAGGCATGAG GATTGGGCCT GAGCCCACCA CAGACTCCTT CATTGCGGTG 
ATGCAGGGAG ACATGGAGGG GATCATCCCT GGGAACGCCC TGGTGGTGGA TCCCAAGAAA 
CCCTTCAGGA AACTCAACGC CTTTGGCAAC GCCTTCTTGA ACAGGTTCGT GTGTGCCCAG 
CTACCTAACC CTGTGCTGGA GAGCATCAGC GTCATCGACA CACCAGGGAT CCTCTCTGGG 
GAGAAGCAGA GGATCAGCCG GGGGTATGAC TTTGCAGCTG TCCTTGAGTG GTTTGCCGAG 
CGGGTTGACC GCATCATTCT GCTCTTCGAT GCCCACAAAC TGGACATCTC TGATGAGTTC 
TCAGAAGTCA TCAAAGCCCT CAAGAACCAC GAGGACAAGA TGCGAGTGGT GCTGAACAAA 
GCTGACCAGA TCGAGACGCA GCAGCTGATG CGGGTGTACG GGGCCCTCAT GTGGTCCTTG 
GGGAAGATCG TGAACACCCC AGAGGTGATC CGGGTCTACA TCGGCTCCTT CTGGTCCCAC 
CCCCTCCTCA TCCCTGACAA CCGGAAGCTC TTTGAGGCTG AGGAACAGGA CCTATTCAGG 
GACATCCAGA GTCTGCCCCG AAATGCTGCC CTGCGCAAGC TCAACGACCT CATCAAAAGG 
GCCAGGCTGG CCAAGGTCCA CGCCTACATC ATCAGCTCTC TGAAGAAGGA GATGCCCTCG 
GTGTTCGGGA AGGACAACAA GAAGAAGGAG CTGGTCAACA ACCTGGCCGA GATCTATGGC 
CGGATCGAGC GGGAGCACCA GATCTCACCT GGGGACTTCC CCAATCTGAA GAGGATGCAG 
GACCAGCTGC AGGCCCAGGA CTTTAGCAAG TTCCAGCCGC TGAAGAGCAA GCTGCTGGAG 
GTAGTGGACG ACATGCTGGC CCATGACATT GCCCAGCTCA TGGTGCTAGT GCGCCAGGAG 
GAGTCACAGC GGCCCATCCA GATGGTGAAG GGCGGAGCGT TCGAGGGCAC CCTGCACGGC 
CCCTTTGGGC ATGGCTATGG GGAGGGGGCT GGAGAAGGTA TCGATGATGC TGAGTGGGTG 
GTGGCCAGGG ACAAGCCCAT GTACGACGAG ATCTTCTACA CCCTGTCACC GGTGGATGGC 
AAGATCACAG GCGCTAATGC CAAGAAGGAG ATGGTGCGCT CCAAGCTGCC CAACAGTGTG 
CTGGGCAAGA TCTGGAAGCT GGCCGACATT GACAAGGATG GCATGCTGGA CGACGACGAG 
TTTGCACTGG CCAACCACCT CATCAAAGTC AAGCTGGAGG GGCACGAGCT GCCCAACGAG 
CTGCCTGCCC ACCTCCTGCC CCCGTCCAAG AGGAAAGTTG CCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055415.1
CDS	471..2078
Translation	MFSWLGTDDRRRKDPEVFQTVSEGLKKLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMQGDMEGIIPGNALVVDPKKPFRKLNAFGNAFLNRFVCAQLPNPVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKMRVVLNKADQIETQQLMRVYGALMWSLGKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPSVFGKDNKKKELVNNLAEIYGRIEREHQISPGDFPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPIQMVKGGAFEGTLHGPFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSVLGKIWKLADIDKDGMLDDDEFALANHLIKVKLEGHELPNELPAHLLPPSKRKVAE

Target ORF information:

RefSeq Version	NM_014600.3
Organism	Homo sapiens(human)
Definition	Homo sapiens EH domain containing 3 (EHD3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014600.3

ORF Insert Sequence:

ATGTTCAGCT GGCTGGGTAC GGACGACCGC CGGAGGAAGG ACCCCGAGGT TTTCCAGACG 
GTGAGTGAGG GGCTCAAGAA ACTCTACAAG AGCAAGCTGC TGCCCTTGGA AGAGCATTAC 
CGCTTCCACG AGTTCCACTC GCCCGCCCTG GAGGATGCCG ACTTCGACAA CAAGCCCATG 
GTTCTGCTGG TGGGCCAGTA CTCCACTGGG AAGACCACCT TCATCAGGTA CCTGCTGGAA 
CAGGACTTCC CAGGCATGAG GATTGGGCCT GAGCCCACCA CAGACTCCTT CATTGCGGTG 
ATGCAGGGAG ACATGGAGGG GATCATCCCT GGGAACGCCC TGGTGGTGGA TCCCAAGAAA 
CCCTTCAGGA AACTCAACGC CTTTGGCAAC GCCTTCTTGA ACAGGTTCGT GTGTGCCCAG 
CTACCTAACC CTGTGCTGGA GAGCATCAGC GTCATCGACA CACCAGGGAT CCTCTCTGGG 
GAGAAGCAGA GGATCAGCCG GGGGTATGAC TTTGCAGCTG TCCTTGAGTG GTTTGCCGAG 
CGGGTTGACC GCATCATTCT GCTCTTCGAT GCCCACAAAC TGGACATCTC TGATGAGTTC 
TCAGAAGTCA TCAAAGCCCT CAAGAACCAC GAGGACAAGA TGCGAGTGGT GCTGAACAAA 
GCTGACCAGA TCGAGACGCA GCAGCTGATG CGGGTGTACG GGGCCCTCAT GTGGTCCTTG 
GGGAAGATCG TGAACACCCC AGAGGTGATC CGGGTCTACA TCGGCTCCTT CTGGTCCCAC 
CCCCTCCTCA TCCCTGACAA CCGGAAGCTC TTTGAGGCTG AGGAACAGGA CCTATTCAGG 
GACATCCAGA GTCTGCCCCG AAATGCTGCC CTGCGCAAGC TCAACGACCT CATCAAAAGG 
GCCAGGCTGG CCAAGGTCCA CGCCTACATC ATCAGCTCTC TGAAGAAGGA GATGCCCTCG 
GTGTTCGGGA AGGACAACAA GAAGAAGGAG CTGGTCAACA ACCTGGCCGA GATCTATGGC 
CGGATCGAGC GGGAGCACCA GATCTCACCT GGGGACTTCC CCAATCTGAA GAGGATGCAG 
GACCAGCTGC AGGCCCAGGA CTTTAGCAAG TTCCAGCCGC TGAAGAGCAA GCTGCTGGAG 
GTAGTGGACG ACATGCTGGC CCATGACATT GCCCAGCTCA TGGTGCTAGT GCGCCAGGAG 
GAGTCACAGC GGCCCATCCA GATGGTGAAG GGCGGAGCGT TCGAGGGCAC CCTGCACGGC 
CCCTTTGGGC ATGGCTATGG GGAGGGGGCT GGAGAAGGTA TCGATGATGC TGAGTGGGTG 
GTGGCCAGGG ACAAGCCCAT GTACGACGAG ATCTTCTACA CCCTGTCACC GGTGGATGGC 
AAGATCACAG GCGCTAATGC CAAGAAGGAG ATGGTGCGCT CCAAGCTGCC CAACAGTGTG 
CTGGGCAAGA TCTGGAAGCT GGCCGACATT GACAAGGATG GCATGCTGGA CGACGACGAG 
TTTGCACTGG CCAACCACCT CATCAAAGTC AAGCTGGAGG GGCACGAGCT GCCCAACGAG 
CTGCCTGCCC ACCTCCTGCC CCCGTCCAAG AGGAAAGTTG CCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EHD3 ( NM_014600.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055415.1 Homo sapiens EH domain containing 3 (EHD3), mRNA.

Price & Availability↑

CloneID	OHu13901
Clone ID Related Accession (Same CDS sequence)	NM_014600.3 , NM_014600.2
Accession Version	NM_014600.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	EH domain-containing protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK316006.1, DA196836.1, BC033100.1 and AF214736.1. On May 8, 2009 this sequence version replaced NM_014600.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.88102.1, SRR1803615.35683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCAGCT GGCTGGGTAC GGACGACCGC CGGAGGAAGG ACCCCGAGGT TTTCCAGACG 
GTGAGTGAGG GGCTCAAGAA ACTCTACAAG AGCAAGCTGC TGCCCTTGGA AGAGCATTAC 
CGCTTCCACG AGTTCCACTC GCCCGCCCTG GAGGATGCCG ACTTCGACAA CAAGCCCATG 
GTTCTGCTGG TGGGCCAGTA CTCCACTGGG AAGACCACCT TCATCAGGTA CCTGCTGGAA 
CAGGACTTCC CAGGCATGAG GATTGGGCCT GAGCCCACCA CAGACTCCTT CATTGCGGTG 
ATGCAGGGAG ACATGGAGGG GATCATCCCT GGGAACGCCC TGGTGGTGGA TCCCAAGAAA 
CCCTTCAGGA AACTCAACGC CTTTGGCAAC GCCTTCTTGA ACAGGTTCGT GTGTGCCCAG 
CTACCTAACC CTGTGCTGGA GAGCATCAGC GTCATCGACA CACCAGGGAT CCTCTCTGGG 
GAGAAGCAGA GGATCAGCCG GGGGTATGAC TTTGCAGCTG TCCTTGAGTG GTTTGCCGAG 
CGGGTTGACC GCATCATTCT GCTCTTCGAT GCCCACAAAC TGGACATCTC TGATGAGTTC 
TCAGAAGTCA TCAAAGCCCT CAAGAACCAC GAGGACAAGA TGCGAGTGGT GCTGAACAAA 
GCTGACCAGA TCGAGACGCA GCAGCTGATG CGGGTGTACG GGGCCCTCAT GTGGTCCTTG 
GGGAAGATCG TGAACACCCC AGAGGTGATC CGGGTCTACA TCGGCTCCTT CTGGTCCCAC 
CCCCTCCTCA TCCCTGACAA CCGGAAGCTC TTTGAGGCTG AGGAACAGGA CCTATTCAGG 
GACATCCAGA GTCTGCCCCG AAATGCTGCC CTGCGCAAGC TCAACGACCT CATCAAAAGG 
GCCAGGCTGG CCAAGGTCCA CGCCTACATC ATCAGCTCTC TGAAGAAGGA GATGCCCTCG 
GTGTTCGGGA AGGACAACAA GAAGAAGGAG CTGGTCAACA ACCTGGCCGA GATCTATGGC 
CGGATCGAGC GGGAGCACCA GATCTCACCT GGGGACTTCC CCAATCTGAA GAGGATGCAG 
GACCAGCTGC AGGCCCAGGA CTTTAGCAAG TTCCAGCCGC TGAAGAGCAA GCTGCTGGAG 
GTAGTGGACG ACATGCTGGC CCATGACATT GCCCAGCTCA TGGTGCTAGT GCGCCAGGAG 
GAGTCACAGC GGCCCATCCA GATGGTGAAG GGCGGAGCGT TCGAGGGCAC CCTGCACGGC 
CCCTTTGGGC ATGGCTATGG GGAGGGGGCT GGAGAAGGTA TCGATGATGC TGAGTGGGTG 
GTGGCCAGGG ACAAGCCCAT GTACGACGAG ATCTTCTACA CCCTGTCACC GGTGGATGGC 
AAGATCACAG GCGCTAATGC CAAGAAGGAG ATGGTGCGCT CCAAGCTGCC CAACAGTGTG 
CTGGGCAAGA TCTGGAAGCT GGCCGACATT GACAAGGATG GCATGCTGGA CGACGACGAG 
TTTGCACTGG CCAACCACCT CATCAAAGTC AAGCTGGAGG GGCACGAGCT GCCCAACGAG 
CTGCCTGCCC ACCTCCTGCC CCCGTCCAAG AGGAAAGTTG CCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055415.1
CDS	609..2216
Translation	MFSWLGTDDRRRKDPEVFQTVSEGLKKLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMQGDMEGIIPGNALVVDPKKPFRKLNAFGNAFLNRFVCAQLPNPVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKMRVVLNKADQIETQQLMRVYGALMWSLGKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPSVFGKDNKKKELVNNLAEIYGRIEREHQISPGDFPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPIQMVKGGAFEGTLHGPFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSVLGKIWKLADIDKDGMLDDDEFALANHLIKVKLEGHELPNELPAHLLPPSKRKVAE

Target ORF information:

RefSeq Version	NM_014600.2
Organism	Homo sapiens(human)
Definition	Homo sapiens EH domain containing 3 (EHD3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014600.2

ORF Insert Sequence:

ATGTTCAGCT GGCTGGGTAC GGACGACCGC CGGAGGAAGG ACCCCGAGGT TTTCCAGACG 
GTGAGTGAGG GGCTCAAGAA ACTCTACAAG AGCAAGCTGC TGCCCTTGGA AGAGCATTAC 
CGCTTCCACG AGTTCCACTC GCCCGCCCTG GAGGATGCCG ACTTCGACAA CAAGCCCATG 
GTTCTGCTGG TGGGCCAGTA CTCCACTGGG AAGACCACCT TCATCAGGTA CCTGCTGGAA 
CAGGACTTCC CAGGCATGAG GATTGGGCCT GAGCCCACCA CAGACTCCTT CATTGCGGTG 
ATGCAGGGAG ACATGGAGGG GATCATCCCT GGGAACGCCC TGGTGGTGGA TCCCAAGAAA 
CCCTTCAGGA AACTCAACGC CTTTGGCAAC GCCTTCTTGA ACAGGTTCGT GTGTGCCCAG 
CTACCTAACC CTGTGCTGGA GAGCATCAGC GTCATCGACA CACCAGGGAT CCTCTCTGGG 
GAGAAGCAGA GGATCAGCCG GGGGTATGAC TTTGCAGCTG TCCTTGAGTG GTTTGCCGAG 
CGGGTTGACC GCATCATTCT GCTCTTCGAT GCCCACAAAC TGGACATCTC TGATGAGTTC 
TCAGAAGTCA TCAAAGCCCT CAAGAACCAC GAGGACAAGA TGCGAGTGGT GCTGAACAAA 
GCTGACCAGA TCGAGACGCA GCAGCTGATG CGGGTGTACG GGGCCCTCAT GTGGTCCTTG 
GGGAAGATCG TGAACACCCC AGAGGTGATC CGGGTCTACA TCGGCTCCTT CTGGTCCCAC 
CCCCTCCTCA TCCCTGACAA CCGGAAGCTC TTTGAGGCTG AGGAACAGGA CCTATTCAGG 
GACATCCAGA GTCTGCCCCG AAATGCTGCC CTGCGCAAGC TCAACGACCT CATCAAAAGG 
GCCAGGCTGG CCAAGGTCCA CGCCTACATC ATCAGCTCTC TGAAGAAGGA GATGCCCTCG 
GTGTTCGGGA AGGACAACAA GAAGAAGGAG CTGGTCAACA ACCTGGCCGA GATCTATGGC 
CGGATCGAGC GGGAGCACCA GATCTCACCT GGGGACTTCC CCAATCTGAA GAGGATGCAG 
GACCAGCTGC AGGCCCAGGA CTTTAGCAAG TTCCAGCCGC TGAAGAGCAA GCTGCTGGAG 
GTAGTGGACG ACATGCTGGC CCATGACATT GCCCAGCTCA TGGTGCTAGT GCGCCAGGAG 
GAGTCACAGC GGCCCATCCA GATGGTGAAG GGCGGAGCGT TCGAGGGCAC CCTGCACGGC 
CCCTTTGGGC ATGGCTATGG GGAGGGGGCT GGAGAAGGTA TCGATGATGC TGAGTGGGTG 
GTGGCCAGGG ACAAGCCCAT GTACGACGAG ATCTTCTACA CCCTGTCACC GGTGGATGGC 
AAGATCACAG GCGCTAATGC CAAGAAGGAG ATGGTGCGCT CCAAGCTGCC CAACAGTGTG 
CTGGGCAAGA TCTGGAAGCT GGCCGACATT GACAAGGATG GCATGCTGGA CGACGACGAG 
TTTGCACTGG CCAACCACCT CATCAAAGTC AAGCTGGAGG GGCACGAGCT GCCCAACGAG 
CTGCCTGCCC ACCTCCTGCC CCCGTCCAAG AGGAAAGTTG CCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EHD3 ( XM_011532806.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531108.1 Homo sapiens EH domain containing 3 (EHD3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu67488
Clone ID Related Accession (Same CDS sequence)	XM_011532806.2
Accession Version	XM_011532806.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	EH domain-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532806.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGTGG TGCTGAACAA AGCTGACCAG ATCGAGACGC AGCAGCTGAT GCGGGTGTAC 
GGGGCCCTCA TGTGGTCCTT GGGGAAGATC GTGAACACCC CAGAGGTGAT CCGGGTCTAC 
ATCGGCTCCT TCTGGTCCCA CCCCCTCCTC ATCCCTGACA ACCGGAAGCT CTTTGAGGCT 
GAGGAACAGG ACCTATTCAG GGACATCCAG AGTCTGCCCC GAAATGCTGC CCTGCGCAAG 
CTCAACGACC TCATCAAAAG GGCCAGGCTG GCCAAGGTCC ACGCCTACAT CATCAGCTCT 
CTGAAGAAGG AGATGCCCTC GGTGTTCGGG AAGGACAACA AGAAGAAGGA GCTGGTCAAC 
AACCTGGCCG AGATCTATGG CCGGATCGAG CGGGAGCACC AGATCTCACC TGGGGACTTC 
CCCAATCTGA AGAGGATGCA GGACCAGCTG CAGGCCCAGG ACTTTAGCAA GTTCCAGCCG 
CTGAAGAGCA AGCTGCTGGA GGTAGTGGAC GACATGCTGG CCCATGACAT TGCCCAGCTC 
ATGGTGCTAG TGCGCCAGGA GGAGTCACAG CGGCCCATCC AGATGGTGAA GGGCGGAGCG 
TTCGAGGGCA CCCTGCACGG CCCCTTTGGG CATGGCTATG GGGAGGGGGC TGGAGAAGGT 
ATCGATGATG CTGAGTGGGT GGTGGCCAGG GACAAGCCCA TGTACGACGA GATCTTCTAC 
ACCCTGTCAC CGGTGGATGG CAAGATCACA GGCGCTAATG CCAAGAAGGA GATGGTGCGC 
TCCAAGCTGC CCAACAGTGT GCTGGGCAAG ATCTGGAAGC TGGCCGACAT TGACAAGGAT 
GGCATGCTGG ACGACGACGA GTTTGCACTG GCCAACCACC TCATCAAAGT CAAGCTGGAG 
GGGCACGAGC TGCCCAACGA GCTGCCTGCC CACCTCCTGC CCCCGTCCAA GAGGAAAGTT 
GCCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531108.1
CDS	212..1180
Translation	MRVVLNKADQIETQQLMRVYGALMWSLGKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPSVFGKDNKKKELVNNLAEIYGRIEREHQISPGDFPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPIQMVKGGAFEGTLHGPFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSVLGKIWKLADIDKDGMLDDDEFALANHLIKVKLEGHELPNELPAHLLPPSKRKVAE

Target ORF information:

RefSeq Version	XM_011532806.2
Organism	Homo sapiens(human)
Definition	Homo sapiens EH domain containing 3 (EHD3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532806.2

ORF Insert Sequence:

ATGCGAGTGG TGCTGAACAA AGCTGACCAG ATCGAGACGC AGCAGCTGAT GCGGGTGTAC 
GGGGCCCTCA TGTGGTCCTT GGGGAAGATC GTGAACACCC CAGAGGTGAT CCGGGTCTAC 
ATCGGCTCCT TCTGGTCCCA CCCCCTCCTC ATCCCTGACA ACCGGAAGCT CTTTGAGGCT 
GAGGAACAGG ACCTATTCAG GGACATCCAG AGTCTGCCCC GAAATGCTGC CCTGCGCAAG 
CTCAACGACC TCATCAAAAG GGCCAGGCTG GCCAAGGTCC ACGCCTACAT CATCAGCTCT 
CTGAAGAAGG AGATGCCCTC GGTGTTCGGG AAGGACAACA AGAAGAAGGA GCTGGTCAAC 
AACCTGGCCG AGATCTATGG CCGGATCGAG CGGGAGCACC AGATCTCACC TGGGGACTTC 
CCCAATCTGA AGAGGATGCA GGACCAGCTG CAGGCCCAGG ACTTTAGCAA GTTCCAGCCG 
CTGAAGAGCA AGCTGCTGGA GGTAGTGGAC GACATGCTGG CCCATGACAT TGCCCAGCTC 
ATGGTGCTAG TGCGCCAGGA GGAGTCACAG CGGCCCATCC AGATGGTGAA GGGCGGAGCG 
TTCGAGGGCA CCCTGCACGG CCCCTTTGGG CATGGCTATG GGGAGGGGGC TGGAGAAGGT 
ATCGATGATG CTGAGTGGGT GGTGGCCAGG GACAAGCCCA TGTACGACGA GATCTTCTAC 
ACCCTGTCAC CGGTGGATGG CAAGATCACA GGCGCTAATG CCAAGAAGGA GATGGTGCGC 
TCCAAGCTGC CCAACAGTGT GCTGGGCAAG ATCTGGAAGC TGGCCGACAT TGACAAGGAT 
GGCATGCTGG ACGACGACGA GTTTGCACTG GCCAACCACC TCATCAAAGT CAAGCTGGAG 
GGGCACGAGC TGCCCAACGA GCTGCCTGCC CACCTCCTGC CCCCGTCCAA GAGGAAAGTT 
GCCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spatio-temporal regulation of EGFR signaling by the Eps15 homology domain-containing protein 3 (EHD3).
Oncotarget7(48)79203-79216(2016 Nov)
Amessou M,Ebrahim AS,Dilly A,Joseph M,Tabolina M,Chukkapalli S,Meroueh L,Syed JT,Liddane A,Lang SL,Al-Katib A,Kandouz M

EHD3 Protein Is Required for Tubular Recycling Endosome Stabilization, and an Asparagine-Glutamic Acid Residue Pair within Its Eps15 Homology (EH) Domain Dictates Its Selective Binding to NPF Peptides.
The Journal of biological chemistry291(26)13465-78(2016 Jun)
Bahl K,Xie S,Spagnol G,Sorgen P,Naslavsky N,Caplan S

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.
Nature cell biology17(3)228-240(2015 Mar)
Lu Q,Insinna C,Ott C,Stauffer J,Pintado PA,Rahajeng J,Baxa U,Walia V,Cuenca A,Hwang YS,Daar IO,Lopes S,Lippincott-Schwartz J,Jackson PK,Caplan S,Westlake CJ

SUMOylation of EHD3 Modulates Tubulation of the Endocytic Recycling Compartment.
PloS one10(7)e0134053(2015)
Cabasso O,Pekar O,Horowitz M

Association between EHD3 gene and the cognitive function of patients with major depressive disorder.
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae36(3)227-33(2014 Jun)
Wang L,Shi CJ,Xu Q

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EHD3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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