HCRT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HCRT
Entrez Gene ID	3060
Full Name	hypocretin neuropeptide precursor
Synonyms	NRCLP1,OX,PPOX
General protein information	Preferred Names hypocretin neuropeptide precursor Names orexin hypocretin (orexin) neuropeptide prepro-orexin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.2
Summary	This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis. [provided by RefSeq, Jan 2010].
Disorder MIM:	602358
Disorder Html:	?Narcolepsy 1, 161400 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001524.1	NP_001515.1	orexin precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HCRT	XP_001166578.1
	HCRT	XP_001108991.1
Homo sapiens (human)	HCRT	NP_001515.1
Bos taurus (cattle)	HCRT	NP_001159992.1
Rattus norvegicus (Norway rat)	Hcrt	NP_037311.1
Mus musculus (house mouse)	Hcrt	NP_034540.1
Canis lupus familiaris (dog)	HCRT	NP_001029166.1

Gene Ontology
Bibliography

Related articles in PubMed

Crystal Structures of Human Orexin 2 Receptor Bound to the Subtype-Selective Antagonist EMPA.
Suno R, Kimura KT, Nakane T, Yamashita K, Wang J, Fujiwara T, Yamanaka Y, Im D, Horita S, Tsujimoto H, Tawaramoto MS, Hirokawa T, Nango E, Tono K, Kameshima T, Hatsui T, Joti Y, Yabashi M, Shimamoto K, Yamamoto M, Rosenbaum DM, Iwata S, Shimamura T, Kobayashi T
Structure (London, England : 1993)26(1)7-19.e5(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Orexin levels do not play a role in the control of nocturnal blood pressure dipping in patients with narcolepsy type 1.
Title: The relationship between orexin levels and blood pressure changes in patients with narcolepsy.

findings show that in few patients with narcolepsy with cataplexy, symptoms and CSF hypocretin-1 and histamine levels can change over time
Title: Temporal Changes in the Cerebrospinal Fluid Level of Hypocretin-1 and Histamine in Narcolepsy.

Exercise induced a significant increase in plasmatic orexin A with a peak at 3o minutes.
Title: Exercise increases the level of plasma orexin A in humans.

Orexin A levels correlated with age in females and were significantly elevated in PWS even after these effects were controlled. These findings support the hypothesis that dysregulation of orexin signaling may contribute to behavioral problems and hyperphagia in PWS.
Title: Higher plasma orexin a levels in children with Prader-Willi syndrome compared with healthy unrelated sibling controls.

Low circulating Orexin A was found to be significantly associated with hyperlipidemia, obesity and obesity-related disorders in North Indian premenopausal women.
Title: Association of Circulating Orexin-A Level With Metabolic Risk Factors in North Indian Pre Menopausal Women.

The following HCRT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCRT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu19912	NM_001524.1 Latest version!	Homo sapiens hypocretin neuropeptide precursor (HCRT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HCRT ( NM_001524.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001515.1 Homo sapiens hypocretin neuropeptide precursor (HCRT), mRNA.

Price & Availability↑

CloneID	OHu19912
Clone ID Related Accession (Same CDS sequence)	NM_001524.1
Accession Version	NM_001524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	orexin precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF041240.1. This sequence is a reference standard in the RefSeqGene project. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF041240.1, AW163085.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000293330.1/ ENSP00000293330.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGAACCTTC CTTCCACAAA GGTCTCCTGG GCCGCCGTGA CGCTACTGCT GCTGCTGCTG 
CTGCTGCCGC CCGCGCTGTT GTCGTCCGGG GCGGCTGCAC AGCCCCTGCC CGACTGCTGT 
CGTCAAAAGA CTTGCTCTTG CCGCCTCTAC GAGCTGCTGC ACGGCGCGGG CAATCACGCG 
GCCGGCATCC TCACGCTGGG CAAGCGGAGG TCCGGGCCCC CGGGCCTCCA GGGTCGGCTG 
CAGCGCCTCC TGCAGGCCAG CGGCAACCAC GCCGCGGGCA TCCTGACCAT GGGCCGCCGC 
GCAGGCGCAG AGCCAGCGCC GCGCCCCTGC CTCGGGCGCC GCTGTTCCGC CCCGGCCGCC 
GCCTCCGTCG CGCCCGGAGG ACAGTCCGGG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001515.1
CDS	88..483
Translation	MNLPSTKVSWAAVTLLLLLLLLPPALLSSGAAAQPLPDCCRQKTCSCRLYELLHGAGNHAAGILTLGKRRSGPPGLQGRLQRLLQASGNHAAGILTMGRRAGAEPAPRPCLGRRCSAPAAASVAPGGQSGI

Target ORF information:

RefSeq Version	NM_001524.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hypocretin neuropeptide precursor (HCRT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001524.1

ORF Insert Sequence:

ATGAACCTTC CTTCCACAAA GGTCTCCTGG GCCGCCGTGA CGCTACTGCT GCTGCTGCTG 
CTGCTGCCGC CCGCGCTGTT GTCGTCCGGG GCGGCTGCAC AGCCCCTGCC CGACTGCTGT 
CGTCAAAAGA CTTGCTCTTG CCGCCTCTAC GAGCTGCTGC ACGGCGCGGG CAATCACGCG 
GCCGGCATCC TCACGCTGGG CAAGCGGAGG TCCGGGCCCC CGGGCCTCCA GGGTCGGCTG 
CAGCGCCTCC TGCAGGCCAG CGGCAACCAC GCCGCGGGCA TCCTGACCAT GGGCCGCCGC 
GCAGGCGCAG AGCCAGCGCC GCGCCCCTGC CTCGGGCGCC GCTGTTCCGC CCCGGCCGCC 
GCCTCCGTCG CGCCCGGAGG ACAGTCCGGG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crystal Structures of Human Orexin 2 Receptor Bound to the Subtype-Selective Antagonist EMPA.
Structure (London, England : 1993)26(1)7-19.e5(2018 Jan)
Suno R,Kimura KT,Nakane T,Yamashita K,Wang J,Fujiwara T,Yamanaka Y,Im D,Horita S,Tsujimoto H,Tawaramoto MS,Hirokawa T,Nango E,Tono K,Kameshima T,Hatsui T,Joti Y,Yabashi M,Shimamoto K,Yamamoto M,Rosenbaum DM,Iwata S,Shimamura T,Kobayashi T

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HCRT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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