ORF » Species Summary » Homo sapiens » HCK cDNA ORF clone

HCK cDNA ORF clone, Homo sapiens(human)

The following HCK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu28515 NM_001172133.2
Latest version!		 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$189.50-$265.30
$379.00
OHu28515 NM_001172129.2
Latest version!		 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$189.50-$265.30
$379.00
OHu28545 NM_002110.4
Latest version!		 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$189.50-$265.30
$379.00
OHu28510 NM_001172132.2
Latest version!		 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $300.30
$429.00
OHu28528 NM_001172131.2
Latest version!		 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OHu28541 NM_001172130.2
Latest version!		 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
View Old Accession Versions >>
OHu28545 NM_002110.3 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$189.50-$265.30
$379.00
OHu28541 NM_001172130.1 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OHu28510 NM_001172132.1 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $300.30
$429.00
OHu28528 NM_001172131.1 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OHu28515 NM_001172133.1 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$189.50-$265.30
$379.00
OHu28515 NM_001172129.1 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    HCK ( NM_001172133.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165604.1 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 4, mRNA.
    Price & Availability↑
    CloneID OHu28515
    Clone ID Related Accession (Same CDS sequence) NM_001172133.1 , NM_001172129.1 , NM_001172133.2 , NM_001172129.2
    Accession Version NM_001172133.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product tyrosine-protein kinase HCK isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA465978.1, AK298726.1 and AW139272.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (4) differs in its 5' UTR and uses the downstream AUG start codon, compared to variant 1, resulting in an isoform (b) that is shorter at the N-terminus, compared to isoform a. Both variants 1 and 4 encode isoform b. CCDS Note: This CCDS, which is supported by the mRNAs AK026432.1, BC108930.1 and others, represents a short human HCK isoform, known as p59HCK, as described in PMID:7791757. This isoform initiates translation from a downstream AUG start codon. Alternative translation initiation from an upstream non-AUG (CUG) start codon, which is well-conserved and present in a strong Kozak signal context, produces an isoform that is 21 aa longer at the N-terminus. The longer isoform, which is known as p61HCK, is represented by CCDS 33460.1. These isoforms exhibit distinct subcellular localization, as indicated in PMID:7791757. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298726.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820689, SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGGTGCA TGAAGTCCAA GTTCCTCCAG GTCGGAGGCA ATACATTCTC AAAAACTGAA 
    ACCAGCGCCA GCCCACACTG TCCTGTGTAC GTGCCGGATC CCACATCCAC CATCAAGCCG 
    GGGCCTAATA GCCACAACAG CAACACACCA GGAATCAGGG AGGCAGGCTC TGAGGACATC 
    ATCGTGGTTG CCCTGTATGA TTACGAGGCC ATTCACCACG AAGACCTCAG CTTCCAGAAG 
    GGGGACCAGA TGGTGGTCCT AGAGGAATCC GGGGAGTGGT GGAAGGCTCG ATCCCTGGCC 
    ACCCGGAAGG AGGGCTACAT CCCAAGCAAC TATGTCGCCC GCGTTGACTC TCTGGAGACA 
    GAGGAGTGGT TTTTCAAGGG CATCAGCCGG AAGGACGCAG AGCGCCAACT GCTGGCTCCC 
    GGCAACATGC TGGGCTCCTT CATGATCCGG GATAGCGAGA CCACTAAAGG AAGCTACTCT 
    TTGTCCGTGC GAGACTACGA CCCTCGGCAG GGAGATACCG TGAAACATTA CAAGATCCGG 
    ACCCTGGACA ACGGGGGCTT CTACATATCC CCCCGAAGCA CCTTCAGCAC TCTGCAGGAG 
    CTGGTGGACC ACTACAAGAA GGGGAACGAC GGGCTCTGCC AGAAACTGTC GGTGCCCTGC 
    ATGTCTTCCA AGCCCCAGAA GCCTTGGGAG AAAGATGCCT GGGAGATCCC TCGGGAATCC 
    CTCAAGCTGG AGAAGAAACT TGGAGCTGGG CAGTTTGGGG AAGTCTGGAT GGCCACCTAC 
    AACAAGCACA CCAAGGTGGC AGTGAAGACG ATGAAGCCAG GGAGCATGTC GGTGGAGGCC 
    TTCCTGGCAG AGGCCAACGT GATGAAAACT CTGCAGCATG ACAAGCTGGT CAAACTTCAT 
    GCGGTGGTCA CCAAGGAGCC CATCTACATC ATCACGGAGT TCATGGCCAA AGGAAGCTTG 
    CTGGACTTTC TGAAAAGTGA TGAGGGCAGC AAGCAGCCAT TGCCAAAACT CATTGACTTC 
    TCAGCCCAGA TTGCAGAAGG CATGGCCTTC ATCGAGCAGA GGAACTACAT CCACCGAGAC 
    CTCCGAGCTG CCAACATCTT GGTCTCTGCA TCCCTGGTGT GTAAGATTGC TGACTTTGGC 
    CTGGCCCGGG TCATTGAGGA CAACGAGTAC ACGGCTCGGG AAGGGGCCAA GTTCCCCATC 
    AAGTGGACAG CTCCTGAAGC CATCAACTTT GGCTCCTTCA CCATCAAGTC AGACGTCTGG 
    TCCTTTGGTA TCCTGCTGAT GGAGATCGTC ACCTACGGCC GGATCCCTTA CCCAGGGATG 
    TCAAACCCTG AAGTGATCCG AGCTCTGGAG CGTGGATACC GGATGCCTCG CCCAGAGAAC 
    TGCCCAGAGG AGCTCTACAA CATCATGATG CGCTGCTGGA AAAACCGTCC GGAGGAGCGG 
    CCGACCTTCG AATACATCCA GAGTGTGCTG GATGACTTCT ACACGGCCAC AGAGAGCCAG 
    TACCAACAGC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165604.1
    CDS365..1882
    Translation

    Target ORF information:

    RefSeq Version NM_001172133.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001172133.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGGTGCA TGAAGTCCAA GTTCCTCCAG GTCGGAGGCA ATACATTCTC AAAAACTGAA 
    ACCAGCGCCA GCCCACACTG TCCTGTGTAC GTGCCGGATC CCACATCCAC CATCAAGCCG 
    GGGCCTAATA GCCACAACAG CAACACACCA GGAATCAGGG AGGCAGGCTC TGAGGACATC 
    ATCGTGGTTG CCCTGTATGA TTACGAGGCC ATTCACCACG AAGACCTCAG CTTCCAGAAG 
    GGGGACCAGA TGGTGGTCCT AGAGGAATCC GGGGAGTGGT GGAAGGCTCG ATCCCTGGCC 
    ACCCGGAAGG AGGGCTACAT CCCAAGCAAC TATGTCGCCC GCGTTGACTC TCTGGAGACA 
    GAGGAGTGGT TTTTCAAGGG CATCAGCCGG AAGGACGCAG AGCGCCAACT GCTGGCTCCC 
    GGCAACATGC TGGGCTCCTT CATGATCCGG GATAGCGAGA CCACTAAAGG AAGCTACTCT 
    TTGTCCGTGC GAGACTACGA CCCTCGGCAG GGAGATACCG TGAAACATTA CAAGATCCGG 
    ACCCTGGACA ACGGGGGCTT CTACATATCC CCCCGAAGCA CCTTCAGCAC TCTGCAGGAG 
    CTGGTGGACC ACTACAAGAA GGGGAACGAC GGGCTCTGCC AGAAACTGTC GGTGCCCTGC 
    ATGTCTTCCA AGCCCCAGAA GCCTTGGGAG AAAGATGCCT GGGAGATCCC TCGGGAATCC 
    CTCAAGCTGG AGAAGAAACT TGGAGCTGGG CAGTTTGGGG AAGTCTGGAT GGCCACCTAC 
    AACAAGCACA CCAAGGTGGC AGTGAAGACG ATGAAGCCAG GGAGCATGTC GGTGGAGGCC 
    TTCCTGGCAG AGGCCAACGT GATGAAAACT CTGCAGCATG ACAAGCTGGT CAAACTTCAT 
    GCGGTGGTCA CCAAGGAGCC CATCTACATC ATCACGGAGT TCATGGCCAA AGGAAGCTTG 
    CTGGACTTTC TGAAAAGTGA TGAGGGCAGC AAGCAGCCAT TGCCAAAACT CATTGACTTC 
    TCAGCCCAGA TTGCAGAAGG CATGGCCTTC ATCGAGCAGA GGAACTACAT CCACCGAGAC 
    CTCCGAGCTG CCAACATCTT GGTCTCTGCA TCCCTGGTGT GTAAGATTGC TGACTTTGGC 
    CTGGCCCGGG TCATTGAGGA CAACGAGTAC ACGGCTCGGG AAGGGGCCAA GTTCCCCATC 
    AAGTGGACAG CTCCTGAAGC CATCAACTTT GGCTCCTTCA CCATCAAGTC AGACGTCTGG 
    TCCTTTGGTA TCCTGCTGAT GGAGATCGTC ACCTACGGCC GGATCCCTTA CCCAGGGATG 
    TCAAACCCTG AAGTGATCCG AGCTCTGGAG CGTGGATACC GGATGCCTCG CCCAGAGAAC 
    TGCCCAGAGG AGCTCTACAA CATCATGATG CGCTGCTGGA AAAACCGTCC GGAGGAGCGG 
    CCGACCTTCG AATACATCCA GAGTGTGCTG GATGACTTCT ACACGGCCAC AGAGAGCCAG 
    TACCAACAGC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    HCK ( NM_001172129.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165600.1 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu28515
    Clone ID Related Accession (Same CDS sequence) NM_001172133.1 , NM_001172129.1 , NM_001172133.2 , NM_001172129.2
    Accession Version NM_001172129.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product tyrosine-protein kinase HCK isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA465978.1, BC114463.1 and AW139272.1. Transcript Variant: This variant (1) encodes two isoforms due to the use of alternative translation initiation codons, as demonstrated in PMIDs 1875927 and 7791757. The longer isoform (a, also known as p61HCK) is derived from an upstream non-AUG (CUG) start codon, while the shorter isoform (b, also known as p59HCK) is derived from a downstream AUG start codon. The shorter isoform (b) is represented in this RefSeq. Both variants 1 and 4 encode isoform b. CCDS Note: This CCDS, which is supported by the mRNAs AK026432.1, BC108930.1 and others, represents a short human HCK isoform, known as p59HCK, as described in PMID:7791757. This isoform initiates translation from a downstream AUG start codon. Alternative translation initiation from an upstream non-AUG (CUG) start codon, which is well-conserved and present in a strong Kozak signal context, produces an isoform that is 21 aa longer at the N-terminus. The longer isoform, which is known as p61HCK, is represented by CCDS 33460.1. These isoforms exhibit distinct subcellular localization, as indicated in PMID:7791757. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK026432.1, SRR1163658.439890.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:7791757) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGGTGCA TGAAGTCCAA GTTCCTCCAG GTCGGAGGCA ATACATTCTC AAAAACTGAA 
    ACCAGCGCCA GCCCACACTG TCCTGTGTAC GTGCCGGATC CCACATCCAC CATCAAGCCG 
    GGGCCTAATA GCCACAACAG CAACACACCA GGAATCAGGG AGGCAGGCTC TGAGGACATC 
    ATCGTGGTTG CCCTGTATGA TTACGAGGCC ATTCACCACG AAGACCTCAG CTTCCAGAAG 
    GGGGACCAGA TGGTGGTCCT AGAGGAATCC GGGGAGTGGT GGAAGGCTCG ATCCCTGGCC 
    ACCCGGAAGG AGGGCTACAT CCCAAGCAAC TATGTCGCCC GCGTTGACTC TCTGGAGACA 
    GAGGAGTGGT TTTTCAAGGG CATCAGCCGG AAGGACGCAG AGCGCCAACT GCTGGCTCCC 
    GGCAACATGC TGGGCTCCTT CATGATCCGG GATAGCGAGA CCACTAAAGG AAGCTACTCT 
    TTGTCCGTGC GAGACTACGA CCCTCGGCAG GGAGATACCG TGAAACATTA CAAGATCCGG 
    ACCCTGGACA ACGGGGGCTT CTACATATCC CCCCGAAGCA CCTTCAGCAC TCTGCAGGAG 
    CTGGTGGACC ACTACAAGAA GGGGAACGAC GGGCTCTGCC AGAAACTGTC GGTGCCCTGC 
    ATGTCTTCCA AGCCCCAGAA GCCTTGGGAG AAAGATGCCT GGGAGATCCC TCGGGAATCC 
    CTCAAGCTGG AGAAGAAACT TGGAGCTGGG CAGTTTGGGG AAGTCTGGAT GGCCACCTAC 
    AACAAGCACA CCAAGGTGGC AGTGAAGACG ATGAAGCCAG GGAGCATGTC GGTGGAGGCC 
    TTCCTGGCAG AGGCCAACGT GATGAAAACT CTGCAGCATG ACAAGCTGGT CAAACTTCAT 
    GCGGTGGTCA CCAAGGAGCC CATCTACATC ATCACGGAGT TCATGGCCAA AGGAAGCTTG 
    CTGGACTTTC TGAAAAGTGA TGAGGGCAGC AAGCAGCCAT TGCCAAAACT CATTGACTTC 
    TCAGCCCAGA TTGCAGAAGG CATGGCCTTC ATCGAGCAGA GGAACTACAT CCACCGAGAC 
    CTCCGAGCTG CCAACATCTT GGTCTCTGCA TCCCTGGTGT GTAAGATTGC TGACTTTGGC 
    CTGGCCCGGG TCATTGAGGA CAACGAGTAC ACGGCTCGGG AAGGGGCCAA GTTCCCCATC 
    AAGTGGACAG CTCCTGAAGC CATCAACTTT GGCTCCTTCA CCATCAAGTC AGACGTCTGG 
    TCCTTTGGTA TCCTGCTGAT GGAGATCGTC ACCTACGGCC GGATCCCTTA CCCAGGGATG 
    TCAAACCCTG AAGTGATCCG AGCTCTGGAG CGTGGATACC GGATGCCTCG CCCAGAGAAC 
    TGCCCAGAGG AGCTCTACAA CATCATGATG CGCTGCTGGA AAAACCGTCC GGAGGAGCGG 
    CCGACCTTCG AATACATCCA GAGTGTGCTG GATGACTTCT ACACGGCCAC AGAGAGCCAG 
    TACCAACAGC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165600.1
    CDS301..1818
    Translation

    Target ORF information:

    RefSeq Version NM_001172129.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001172129.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGGTGCA TGAAGTCCAA GTTCCTCCAG GTCGGAGGCA ATACATTCTC AAAAACTGAA 
    ACCAGCGCCA GCCCACACTG TCCTGTGTAC GTGCCGGATC CCACATCCAC CATCAAGCCG 
    GGGCCTAATA GCCACAACAG CAACACACCA GGAATCAGGG AGGCAGGCTC TGAGGACATC 
    ATCGTGGTTG CCCTGTATGA TTACGAGGCC ATTCACCACG AAGACCTCAG CTTCCAGAAG 
    GGGGACCAGA TGGTGGTCCT AGAGGAATCC GGGGAGTGGT GGAAGGCTCG ATCCCTGGCC 
    ACCCGGAAGG AGGGCTACAT CCCAAGCAAC TATGTCGCCC GCGTTGACTC TCTGGAGACA 
    GAGGAGTGGT TTTTCAAGGG CATCAGCCGG AAGGACGCAG AGCGCCAACT GCTGGCTCCC 
    GGCAACATGC TGGGCTCCTT CATGATCCGG GATAGCGAGA CCACTAAAGG AAGCTACTCT 
    TTGTCCGTGC GAGACTACGA CCCTCGGCAG GGAGATACCG TGAAACATTA CAAGATCCGG 
    ACCCTGGACA ACGGGGGCTT CTACATATCC CCCCGAAGCA CCTTCAGCAC TCTGCAGGAG 
    CTGGTGGACC ACTACAAGAA GGGGAACGAC GGGCTCTGCC AGAAACTGTC GGTGCCCTGC 
    ATGTCTTCCA AGCCCCAGAA GCCTTGGGAG AAAGATGCCT GGGAGATCCC TCGGGAATCC 
    CTCAAGCTGG AGAAGAAACT TGGAGCTGGG CAGTTTGGGG AAGTCTGGAT GGCCACCTAC 
    AACAAGCACA CCAAGGTGGC AGTGAAGACG ATGAAGCCAG GGAGCATGTC GGTGGAGGCC 
    TTCCTGGCAG AGGCCAACGT GATGAAAACT CTGCAGCATG ACAAGCTGGT CAAACTTCAT 
    GCGGTGGTCA CCAAGGAGCC CATCTACATC ATCACGGAGT TCATGGCCAA AGGAAGCTTG 
    CTGGACTTTC TGAAAAGTGA TGAGGGCAGC AAGCAGCCAT TGCCAAAACT CATTGACTTC 
    TCAGCCCAGA TTGCAGAAGG CATGGCCTTC ATCGAGCAGA GGAACTACAT CCACCGAGAC 
    CTCCGAGCTG CCAACATCTT GGTCTCTGCA TCCCTGGTGT GTAAGATTGC TGACTTTGGC 
    CTGGCCCGGG TCATTGAGGA CAACGAGTAC ACGGCTCGGG AAGGGGCCAA GTTCCCCATC 
    AAGTGGACAG CTCCTGAAGC CATCAACTTT GGCTCCTTCA CCATCAAGTC AGACGTCTGG 
    TCCTTTGGTA TCCTGCTGAT GGAGATCGTC ACCTACGGCC GGATCCCTTA CCCAGGGATG 
    TCAAACCCTG AAGTGATCCG AGCTCTGGAG CGTGGATACC GGATGCCTCG CCCAGAGAAC 
    TGCCCAGAGG AGCTCTACAA CATCATGATG CGCTGCTGGA AAAACCGTCC GGAGGAGCGG 
    CCGACCTTCG AATACATCCA GAGTGTGCTG GATGACTTCT ACACGGCCAC AGAGAGCCAG 
    TACCAACAGC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    HCK ( NM_001172133.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165604.1 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 4, mRNA.
    Price & Availability↑
    CloneID OHu28515
    Clone ID Related Accession (Same CDS sequence) NM_001172133.1 , NM_001172129.1 , NM_001172133.2 , NM_001172129.2
    Accession Version NM_001172133.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Homo sapiens(human)
    Product tyrosine-protein kinase HCK isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK298726.1 and AW139272.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001172133.1. Transcript Variant: This variant (4) differs in its 5' UTR and uses the downstream AUG start codon, compared to variant 1, resulting in an isoform (b) that is shorter at the N-terminus, compared to isoform a. Both variants 1 and 4 encode isoform b. CCDS Note: This CCDS, which is supported by the mRNAs AK026432.1, BC108930.1 and others, represents a short human HCK isoform, known as p59HCK, as described in PMID:7791757. This isoform initiates translation from a downstream AUG start codon. Alternative translation initiation from an upstream non-AUG (CUG) start codon, which is well-conserved and present in a strong Kozak signal context, produces an isoform that is 21 aa longer at the N-terminus. The longer isoform, which is known as p61HCK, is represented by CCDS 33460.1. These isoforms exhibit distinct subcellular localization, as indicated in PMID:7791757. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298726.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820689, SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGGTGCA TGAAGTCCAA GTTCCTCCAG GTCGGAGGCA ATACATTCTC AAAAACTGAA 
    ACCAGCGCCA GCCCACACTG TCCTGTGTAC GTGCCGGATC CCACATCCAC CATCAAGCCG 
    GGGCCTAATA GCCACAACAG CAACACACCA GGAATCAGGG AGGCAGGCTC TGAGGACATC 
    ATCGTGGTTG CCCTGTATGA TTACGAGGCC ATTCACCACG AAGACCTCAG CTTCCAGAAG 
    GGGGACCAGA TGGTGGTCCT AGAGGAATCC GGGGAGTGGT GGAAGGCTCG ATCCCTGGCC 
    ACCCGGAAGG AGGGCTACAT CCCAAGCAAC TATGTCGCCC GCGTTGACTC TCTGGAGACA 
    GAGGAGTGGT TTTTCAAGGG CATCAGCCGG AAGGACGCAG AGCGCCAACT GCTGGCTCCC 
    GGCAACATGC TGGGCTCCTT CATGATCCGG GATAGCGAGA CCACTAAAGG AAGCTACTCT 
    TTGTCCGTGC GAGACTACGA CCCTCGGCAG GGAGATACCG TGAAACATTA CAAGATCCGG 
    ACCCTGGACA ACGGGGGCTT CTACATATCC CCCCGAAGCA CCTTCAGCAC TCTGCAGGAG 
    CTGGTGGACC ACTACAAGAA GGGGAACGAC GGGCTCTGCC AGAAACTGTC GGTGCCCTGC 
    ATGTCTTCCA AGCCCCAGAA GCCTTGGGAG AAAGATGCCT GGGAGATCCC TCGGGAATCC 
    CTCAAGCTGG AGAAGAAACT TGGAGCTGGG CAGTTTGGGG AAGTCTGGAT GGCCACCTAC 
    AACAAGCACA CCAAGGTGGC AGTGAAGACG ATGAAGCCAG GGAGCATGTC GGTGGAGGCC 
    TTCCTGGCAG AGGCCAACGT GATGAAAACT CTGCAGCATG ACAAGCTGGT CAAACTTCAT 
    GCGGTGGTCA CCAAGGAGCC CATCTACATC ATCACGGAGT TCATGGCCAA AGGAAGCTTG 
    CTGGACTTTC TGAAAAGTGA TGAGGGCAGC AAGCAGCCAT TGCCAAAACT CATTGACTTC 
    TCAGCCCAGA TTGCAGAAGG CATGGCCTTC ATCGAGCAGA GGAACTACAT CCACCGAGAC 
    CTCCGAGCTG CCAACATCTT GGTCTCTGCA TCCCTGGTGT GTAAGATTGC TGACTTTGGC 
    CTGGCCCGGG TCATTGAGGA CAACGAGTAC ACGGCTCGGG AAGGGGCCAA GTTCCCCATC 
    AAGTGGACAG CTCCTGAAGC CATCAACTTT GGCTCCTTCA CCATCAAGTC AGACGTCTGG 
    TCCTTTGGTA TCCTGCTGAT GGAGATCGTC ACCTACGGCC GGATCCCTTA CCCAGGGATG 
    TCAAACCCTG AAGTGATCCG AGCTCTGGAG CGTGGATACC GGATGCCTCG CCCAGAGAAC 
    TGCCCAGAGG AGCTCTACAA CATCATGATG CGCTGCTGGA AAAACCGTCC GGAGGAGCGG 
    CCGACCTTCG AATACATCCA GAGTGTGCTG GATGACTTCT ACACGGCCAC AGAGAGCCAG 
    TACCAACAGC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165604.1
    CDS311..1828
    Translation

    Target ORF information:

    RefSeq Version NM_001172133.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001172133.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGGTGCA TGAAGTCCAA GTTCCTCCAG GTCGGAGGCA ATACATTCTC AAAAACTGAA 
    ACCAGCGCCA GCCCACACTG TCCTGTGTAC GTGCCGGATC CCACATCCAC CATCAAGCCG 
    GGGCCTAATA GCCACAACAG CAACACACCA GGAATCAGGG AGGCAGGCTC TGAGGACATC 
    ATCGTGGTTG CCCTGTATGA TTACGAGGCC ATTCACCACG AAGACCTCAG CTTCCAGAAG 
    GGGGACCAGA TGGTGGTCCT AGAGGAATCC GGGGAGTGGT GGAAGGCTCG ATCCCTGGCC 
    ACCCGGAAGG AGGGCTACAT CCCAAGCAAC TATGTCGCCC GCGTTGACTC TCTGGAGACA 
    GAGGAGTGGT TTTTCAAGGG CATCAGCCGG AAGGACGCAG AGCGCCAACT GCTGGCTCCC 
    GGCAACATGC TGGGCTCCTT CATGATCCGG GATAGCGAGA CCACTAAAGG AAGCTACTCT 
    TTGTCCGTGC GAGACTACGA CCCTCGGCAG GGAGATACCG TGAAACATTA CAAGATCCGG 
    ACCCTGGACA ACGGGGGCTT CTACATATCC CCCCGAAGCA CCTTCAGCAC TCTGCAGGAG 
    CTGGTGGACC ACTACAAGAA GGGGAACGAC GGGCTCTGCC AGAAACTGTC GGTGCCCTGC 
    ATGTCTTCCA AGCCCCAGAA GCCTTGGGAG AAAGATGCCT GGGAGATCCC TCGGGAATCC 
    CTCAAGCTGG AGAAGAAACT TGGAGCTGGG CAGTTTGGGG AAGTCTGGAT GGCCACCTAC 
    AACAAGCACA CCAAGGTGGC AGTGAAGACG ATGAAGCCAG GGAGCATGTC GGTGGAGGCC 
    TTCCTGGCAG AGGCCAACGT GATGAAAACT CTGCAGCATG ACAAGCTGGT CAAACTTCAT 
    GCGGTGGTCA CCAAGGAGCC CATCTACATC ATCACGGAGT TCATGGCCAA AGGAAGCTTG 
    CTGGACTTTC TGAAAAGTGA TGAGGGCAGC AAGCAGCCAT TGCCAAAACT CATTGACTTC 
    TCAGCCCAGA TTGCAGAAGG CATGGCCTTC ATCGAGCAGA GGAACTACAT CCACCGAGAC 
    CTCCGAGCTG CCAACATCTT GGTCTCTGCA TCCCTGGTGT GTAAGATTGC TGACTTTGGC 
    CTGGCCCGGG TCATTGAGGA CAACGAGTAC ACGGCTCGGG AAGGGGCCAA GTTCCCCATC 
    AAGTGGACAG CTCCTGAAGC CATCAACTTT GGCTCCTTCA CCATCAAGTC AGACGTCTGG 
    TCCTTTGGTA TCCTGCTGAT GGAGATCGTC ACCTACGGCC GGATCCCTTA CCCAGGGATG 
    TCAAACCCTG AAGTGATCCG AGCTCTGGAG CGTGGATACC GGATGCCTCG CCCAGAGAAC 
    TGCCCAGAGG AGCTCTACAA CATCATGATG CGCTGCTGGA AAAACCGTCC GGAGGAGCGG 
    CCGACCTTCG AATACATCCA GAGTGTGCTG GATGACTTCT ACACGGCCAC AGAGAGCCAG 
    TACCAACAGC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    HCK ( NM_001172129.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165600.1 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu28515
    Clone ID Related Accession (Same CDS sequence) NM_001172133.1 , NM_001172129.1 , NM_001172133.2 , NM_001172129.2
    Accession Version NM_001172129.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Homo sapiens(human)
    Product tyrosine-protein kinase HCK isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA465978.1, BC114463.1 and AW139272.1. On Jun 2, 2019 this sequence version replaced NM_001172129.1. Transcript Variant: This variant (1) encodes two isoforms due to the use of alternative translation initiation codons, as demonstrated in PMIDs 1875927 and 7791757. The longer isoform (a, also known as p61HCK) is derived from an upstream non-AUG (CUG) start codon, while the shorter isoform (b, also known as p59HCK) is derived from a downstream AUG start codon. The shorter isoform (b) is represented in this RefSeq. Both variants 1 and 4 encode isoform b. CCDS Note: This CCDS, which is supported by the mRNAs AK026432.1, BC108930.1 and others, represents a short human HCK isoform, known as p59HCK, as described in PMID:7791757. This isoform initiates translation from a downstream AUG start codon. Alternative translation initiation from an upstream non-AUG (CUG) start codon, which is well-conserved and present in a strong Kozak signal context, produces an isoform that is 21 aa longer at the N-terminus. The longer isoform, which is known as p61HCK, is represented by CCDS 33460.1. These isoforms exhibit distinct subcellular localization, as indicated in PMID:7791757. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225819.1, BC014435.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:7791757) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGGTGCA TGAAGTCCAA GTTCCTCCAG GTCGGAGGCA ATACATTCTC AAAAACTGAA 
    ACCAGCGCCA GCCCACACTG TCCTGTGTAC GTGCCGGATC CCACATCCAC CATCAAGCCG 
    GGGCCTAATA GCCACAACAG CAACACACCA GGAATCAGGG AGGCAGGCTC TGAGGACATC 
    ATCGTGGTTG CCCTGTATGA TTACGAGGCC ATTCACCACG AAGACCTCAG CTTCCAGAAG 
    GGGGACCAGA TGGTGGTCCT AGAGGAATCC GGGGAGTGGT GGAAGGCTCG ATCCCTGGCC 
    ACCCGGAAGG AGGGCTACAT CCCAAGCAAC TATGTCGCCC GCGTTGACTC TCTGGAGACA 
    GAGGAGTGGT TTTTCAAGGG CATCAGCCGG AAGGACGCAG AGCGCCAACT GCTGGCTCCC 
    GGCAACATGC TGGGCTCCTT CATGATCCGG GATAGCGAGA CCACTAAAGG AAGCTACTCT 
    TTGTCCGTGC GAGACTACGA CCCTCGGCAG GGAGATACCG TGAAACATTA CAAGATCCGG 
    ACCCTGGACA ACGGGGGCTT CTACATATCC CCCCGAAGCA CCTTCAGCAC TCTGCAGGAG 
    CTGGTGGACC ACTACAAGAA GGGGAACGAC GGGCTCTGCC AGAAACTGTC GGTGCCCTGC 
    ATGTCTTCCA AGCCCCAGAA GCCTTGGGAG AAAGATGCCT GGGAGATCCC TCGGGAATCC 
    CTCAAGCTGG AGAAGAAACT TGGAGCTGGG CAGTTTGGGG AAGTCTGGAT GGCCACCTAC 
    AACAAGCACA CCAAGGTGGC AGTGAAGACG ATGAAGCCAG GGAGCATGTC GGTGGAGGCC 
    TTCCTGGCAG AGGCCAACGT GATGAAAACT CTGCAGCATG ACAAGCTGGT CAAACTTCAT 
    GCGGTGGTCA CCAAGGAGCC CATCTACATC ATCACGGAGT TCATGGCCAA AGGAAGCTTG 
    CTGGACTTTC TGAAAAGTGA TGAGGGCAGC AAGCAGCCAT TGCCAAAACT CATTGACTTC 
    TCAGCCCAGA TTGCAGAAGG CATGGCCTTC ATCGAGCAGA GGAACTACAT CCACCGAGAC 
    CTCCGAGCTG CCAACATCTT GGTCTCTGCA TCCCTGGTGT GTAAGATTGC TGACTTTGGC 
    CTGGCCCGGG TCATTGAGGA CAACGAGTAC ACGGCTCGGG AAGGGGCCAA GTTCCCCATC 
    AAGTGGACAG CTCCTGAAGC CATCAACTTT GGCTCCTTCA CCATCAAGTC AGACGTCTGG 
    TCCTTTGGTA TCCTGCTGAT GGAGATCGTC ACCTACGGCC GGATCCCTTA CCCAGGGATG 
    TCAAACCCTG AAGTGATCCG AGCTCTGGAG CGTGGATACC GGATGCCTCG CCCAGAGAAC 
    TGCCCAGAGG AGCTCTACAA CATCATGATG CGCTGCTGGA AAAACCGTCC GGAGGAGCGG 
    CCGACCTTCG AATACATCCA GAGTGTGCTG GATGACTTCT ACACGGCCAC AGAGAGCCAG 
    TACCAACAGC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165600.1
    CDS247..1764
    Translation

    Target ORF information:

    RefSeq Version NM_001172129.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001172129.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGGTGCA TGAAGTCCAA GTTCCTCCAG GTCGGAGGCA ATACATTCTC AAAAACTGAA 
    ACCAGCGCCA GCCCACACTG TCCTGTGTAC GTGCCGGATC CCACATCCAC CATCAAGCCG 
    GGGCCTAATA GCCACAACAG CAACACACCA GGAATCAGGG AGGCAGGCTC TGAGGACATC 
    ATCGTGGTTG CCCTGTATGA TTACGAGGCC ATTCACCACG AAGACCTCAG CTTCCAGAAG 
    GGGGACCAGA TGGTGGTCCT AGAGGAATCC GGGGAGTGGT GGAAGGCTCG ATCCCTGGCC 
    ACCCGGAAGG AGGGCTACAT CCCAAGCAAC TATGTCGCCC GCGTTGACTC TCTGGAGACA 
    GAGGAGTGGT TTTTCAAGGG CATCAGCCGG AAGGACGCAG AGCGCCAACT GCTGGCTCCC 
    GGCAACATGC TGGGCTCCTT CATGATCCGG GATAGCGAGA CCACTAAAGG AAGCTACTCT 
    TTGTCCGTGC GAGACTACGA CCCTCGGCAG GGAGATACCG TGAAACATTA CAAGATCCGG 
    ACCCTGGACA ACGGGGGCTT CTACATATCC CCCCGAAGCA CCTTCAGCAC TCTGCAGGAG 
    CTGGTGGACC ACTACAAGAA GGGGAACGAC GGGCTCTGCC AGAAACTGTC GGTGCCCTGC 
    ATGTCTTCCA AGCCCCAGAA GCCTTGGGAG AAAGATGCCT GGGAGATCCC TCGGGAATCC 
    CTCAAGCTGG AGAAGAAACT TGGAGCTGGG CAGTTTGGGG AAGTCTGGAT GGCCACCTAC 
    AACAAGCACA CCAAGGTGGC AGTGAAGACG ATGAAGCCAG GGAGCATGTC GGTGGAGGCC 
    TTCCTGGCAG AGGCCAACGT GATGAAAACT CTGCAGCATG ACAAGCTGGT CAAACTTCAT 
    GCGGTGGTCA CCAAGGAGCC CATCTACATC ATCACGGAGT TCATGGCCAA AGGAAGCTTG 
    CTGGACTTTC TGAAAAGTGA TGAGGGCAGC AAGCAGCCAT TGCCAAAACT CATTGACTTC 
    TCAGCCCAGA TTGCAGAAGG CATGGCCTTC ATCGAGCAGA GGAACTACAT CCACCGAGAC 
    CTCCGAGCTG CCAACATCTT GGTCTCTGCA TCCCTGGTGT GTAAGATTGC TGACTTTGGC 
    CTGGCCCGGG TCATTGAGGA CAACGAGTAC ACGGCTCGGG AAGGGGCCAA GTTCCCCATC 
    AAGTGGACAG CTCCTGAAGC CATCAACTTT GGCTCCTTCA CCATCAAGTC AGACGTCTGG 
    TCCTTTGGTA TCCTGCTGAT GGAGATCGTC ACCTACGGCC GGATCCCTTA CCCAGGGATG 
    TCAAACCCTG AAGTGATCCG AGCTCTGGAG CGTGGATACC GGATGCCTCG CCCAGAGAAC 
    TGCCCAGAGG AGCTCTACAA CATCATGATG CGCTGCTGGA AAAACCGTCC GGAGGAGCGG 
    CCGACCTTCG AATACATCCA GAGTGTGCTG GATGACTTCT ACACGGCCAC AGAGAGCCAG 
    TACCAACAGC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    HCK ( NM_002110.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002101.2 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu28545
    Clone ID Related Accession (Same CDS sequence) NM_002110.3 , NM_002110.4
    Accession Version NM_002110.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1581bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-27
    Organism Homo sapiens(human)
    Product tyrosine-protein kinase HCK isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA465978.1, BC114463.1 and AW139272.1. On Feb 4, 2010 this sequence version replaced NM_002110.2. Transcript Variant: This variant (1) encodes two isoforms due to the use of alternative translation initiation codons, as demonstrated in PMIDs 1875927 and 7791757. The longer isoform (a, also known as p61HCK) is derived from an upstream non-AUG (CUG) start codon, while the shorter isoform (b, also known as p59HCK) is derived from a downstream AUG start codon. The longer isoform (a) is represented in this RefSeq. CCDS Note: This CCDS, which is supported by the mRNAs AK026432.1, BC108930.1 and others, represents a long human HCK isoform, known as p61HCK, as described in PMIDs 1875927 and 7791757. This isoform initiates translation from a non-AUG (CUG) start codon that is well-conserved and present in a strong Kozak signal context. Alternative translation initiation from a downstream AUG start codon produces an isoform that is 21 aa shorter at the N-terminus. The shorter isoform, which is known as p59HCK, is represented by CCDS 54455.1. These isoforms exhibit distinct subcellular distributions, as indicated in PMID:7791757. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK026432.1, SRR1163658.439890.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 7791757, 1875927 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    CTGGGGGGGC GCTCAAGCTG CGAGGATCCG GGCTGCCCGC GAGACGAGGA GCGGGCGCCC 
    AGGATGGGGT GCATGAAGTC CAAGTTCCTC CAGGTCGGAG GCAATACATT CTCAAAAACT 
    GAAACCAGCG CCAGCCCACA CTGTCCTGTG TACGTGCCGG ATCCCACATC CACCATCAAG 
    CCGGGGCCTA ATAGCCACAA CAGCAACACA CCAGGAATCA GGGAGGCAGG CTCTGAGGAC 
    ATCATCGTGG TTGCCCTGTA TGATTACGAG GCCATTCACC ACGAAGACCT CAGCTTCCAG 
    AAGGGGGACC AGATGGTGGT CCTAGAGGAA TCCGGGGAGT GGTGGAAGGC TCGATCCCTG 
    GCCACCCGGA AGGAGGGCTA CATCCCAAGC AACTATGTCG CCCGCGTTGA CTCTCTGGAG 
    ACAGAGGAGT GGTTTTTCAA GGGCATCAGC CGGAAGGACG CAGAGCGCCA ACTGCTGGCT 
    CCCGGCAACA TGCTGGGCTC CTTCATGATC CGGGATAGCG AGACCACTAA AGGAAGCTAC 
    TCTTTGTCCG TGCGAGACTA CGACCCTCGG CAGGGAGATA CCGTGAAACA TTACAAGATC 
    CGGACCCTGG ACAACGGGGG CTTCTACATA TCCCCCCGAA GCACCTTCAG CACTCTGCAG 
    GAGCTGGTGG ACCACTACAA GAAGGGGAAC GACGGGCTCT GCCAGAAACT GTCGGTGCCC 
    TGCATGTCTT CCAAGCCCCA GAAGCCTTGG GAGAAAGATG CCTGGGAGAT CCCTCGGGAA 
    TCCCTCAAGC TGGAGAAGAA ACTTGGAGCT GGGCAGTTTG GGGAAGTCTG GATGGCCACC 
    TACAACAAGC ACACCAAGGT GGCAGTGAAG ACGATGAAGC CAGGGAGCAT GTCGGTGGAG 
    GCCTTCCTGG CAGAGGCCAA CGTGATGAAA ACTCTGCAGC ATGACAAGCT GGTCAAACTT 
    CATGCGGTGG TCACCAAGGA GCCCATCTAC ATCATCACGG AGTTCATGGC CAAAGGAAGC 
    TTGCTGGACT TTCTGAAAAG TGATGAGGGC AGCAAGCAGC CATTGCCAAA ACTCATTGAC 
    TTCTCAGCCC AGATTGCAGA AGGCATGGCC TTCATCGAGC AGAGGAACTA CATCCACCGA 
    GACCTCCGAG CTGCCAACAT CTTGGTCTCT GCATCCCTGG TGTGTAAGAT TGCTGACTTT 
    GGCCTGGCCC GGGTCATTGA GGACAACGAG TACACGGCTC GGGAAGGGGC CAAGTTCCCC 
    ATCAAGTGGA CAGCTCCTGA AGCCATCAAC TTTGGCTCCT TCACCATCAA GTCAGACGTC 
    TGGTCCTTTG GTATCCTGCT GATGGAGATC GTCACCTACG GCCGGATCCC TTACCCAGGG 
    ATGTCAAACC CTGAAGTGAT CCGAGCTCTG GAGCGTGGAT ACCGGATGCC TCGCCCAGAG 
    AACTGCCCAG AGGAGCTCTA CAACATCATG ATGCGCTGCT GGAAAAACCG TCCGGAGGAG 
    CGGCCGACCT TCGAATACAT CCAGAGTGTG CTGGATGACT TCTACACGGC CACAGAGAGC 
    CAGTACCAAC AGCAGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002101.2
    CDS238..1818
    Translation

    Target ORF information:

    RefSeq Version NM_002110.3
    Organism Homo sapiens(human)
    Definition Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_002110.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    CTGGGGGGGC GCTCAAGCTG CGAGGATCCG GGCTGCCCGC GAGACGAGGA GCGGGCGCCC 
    AGGATGGGGT GCATGAAGTC CAAGTTCCTC CAGGTCGGAG GCAATACATT CTCAAAAACT 
    GAAACCAGCG CCAGCCCACA CTGTCCTGTG TACGTGCCGG ATCCCACATC CACCATCAAG 
    CCGGGGCCTA ATAGCCACAA CAGCAACACA CCAGGAATCA GGGAGGCAGG CTCTGAGGAC 
    ATCATCGTGG TTGCCCTGTA TGATTACGAG GCCATTCACC ACGAAGACCT CAGCTTCCAG 
    AAGGGGGACC AGATGGTGGT CCTAGAGGAA TCCGGGGAGT GGTGGAAGGC TCGATCCCTG 
    GCCACCCGGA AGGAGGGCTA CATCCCAAGC AACTATGTCG CCCGCGTTGA CTCTCTGGAG 
    ACAGAGGAGT GGTTTTTCAA GGGCATCAGC CGGAAGGACG CAGAGCGCCA ACTGCTGGCT 
    CCCGGCAACA TGCTGGGCTC CTTCATGATC CGGGATAGCG AGACCACTAA AGGAAGCTAC 
    TCTTTGTCCG TGCGAGACTA CGACCCTCGG CAGGGAGATA CCGTGAAACA TTACAAGATC 
    CGGACCCTGG ACAACGGGGG CTTCTACATA TCCCCCCGAA GCACCTTCAG CACTCTGCAG 
    GAGCTGGTGG ACCACTACAA GAAGGGGAAC GACGGGCTCT GCCAGAAACT GTCGGTGCCC 
    TGCATGTCTT CCAAGCCCCA GAAGCCTTGG GAGAAAGATG CCTGGGAGAT CCCTCGGGAA 
    TCCCTCAAGC TGGAGAAGAA ACTTGGAGCT GGGCAGTTTG GGGAAGTCTG GATGGCCACC 
    TACAACAAGC ACACCAAGGT GGCAGTGAAG ACGATGAAGC CAGGGAGCAT GTCGGTGGAG 
    GCCTTCCTGG CAGAGGCCAA CGTGATGAAA ACTCTGCAGC ATGACAAGCT GGTCAAACTT 
    CATGCGGTGG TCACCAAGGA GCCCATCTAC ATCATCACGG AGTTCATGGC CAAAGGAAGC 
    TTGCTGGACT TTCTGAAAAG TGATGAGGGC AGCAAGCAGC CATTGCCAAA ACTCATTGAC 
    TTCTCAGCCC AGATTGCAGA AGGCATGGCC TTCATCGAGC AGAGGAACTA CATCCACCGA 
    GACCTCCGAG CTGCCAACAT CTTGGTCTCT GCATCCCTGG TGTGTAAGAT TGCTGACTTT 
    GGCCTGGCCC GGGTCATTGA GGACAACGAG TACACGGCTC GGGAAGGGGC CAAGTTCCCC 
    ATCAAGTGGA CAGCTCCTGA AGCCATCAAC TTTGGCTCCT TCACCATCAA GTCAGACGTC 
    TGGTCCTTTG GTATCCTGCT GATGGAGATC GTCACCTACG GCCGGATCCC TTACCCAGGG 
    ATGTCAAACC CTGAAGTGAT CCGAGCTCTG GAGCGTGGAT ACCGGATGCC TCGCCCAGAG 
    AACTGCCCAG AGGAGCTCTA CAACATCATG ATGCGCTGCT GGAAAAACCG TCCGGAGGAG 
    CGGCCGACCT TCGAATACAT CCAGAGTGTG CTGGATGACT TCTACACGGC CACAGAGAGC 
    CAGTACCAAC AGCAGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    HCK ( NM_002110.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002101.2 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu28545
    Clone ID Related Accession (Same CDS sequence) NM_002110.3 , NM_002110.4
    Accession Version NM_002110.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1581bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Homo sapiens(human)
    Product tyrosine-protein kinase HCK isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA465978.1, BC114463.1 and AW139272.1. On Nov 22, 2018 this sequence version replaced NM_002110.3. Transcript Variant: This variant (1) encodes two isoforms due to the use of alternative translation initiation codons, as demonstrated in PMIDs 1875927 and 7791757. The longer isoform (a, also known as p61HCK) is derived from an upstream non-AUG (CUG) start codon, while the shorter isoform (b, also known as p59HCK) is derived from a downstream AUG start codon. The longer isoform (a) is represented in this RefSeq. CCDS Note: This CCDS, which is supported by the mRNAs AK026432.1, BC108930.1 and others, represents a long human HCK isoform, known as p61HCK, as described in PMIDs 1875927 and 7791757. This isoform initiates translation from a non-AUG (CUG) start codon that is well-conserved and present in a strong Kozak signal context. Alternative translation initiation from a downstream AUG start codon produces an isoform that is 21 aa shorter at the N-terminus. The shorter isoform, which is known as p59HCK, is represented by CCDS 54455.1. These isoforms exhibit distinct subcellular distributions, as indicated in PMID:7791757. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225819.1, SRR3476690.398588.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 7791757, 1875927 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    CTGGGGGGGC GCTCAAGCTG CGAGGATCCG GGCTGCCCGC GAGACGAGGA GCGGGCGCCC 
    AGGATGGGGT GCATGAAGTC CAAGTTCCTC CAGGTCGGAG GCAATACATT CTCAAAAACT 
    GAAACCAGCG CCAGCCCACA CTGTCCTGTG TACGTGCCGG ATCCCACATC CACCATCAAG 
    CCGGGGCCTA ATAGCCACAA CAGCAACACA CCAGGAATCA GGGAGGCAGG CTCTGAGGAC 
    ATCATCGTGG TTGCCCTGTA TGATTACGAG GCCATTCACC ACGAAGACCT CAGCTTCCAG 
    AAGGGGGACC AGATGGTGGT CCTAGAGGAA TCCGGGGAGT GGTGGAAGGC TCGATCCCTG 
    GCCACCCGGA AGGAGGGCTA CATCCCAAGC AACTATGTCG CCCGCGTTGA CTCTCTGGAG 
    ACAGAGGAGT GGTTTTTCAA GGGCATCAGC CGGAAGGACG CAGAGCGCCA ACTGCTGGCT 
    CCCGGCAACA TGCTGGGCTC CTTCATGATC CGGGATAGCG AGACCACTAA AGGAAGCTAC 
    TCTTTGTCCG TGCGAGACTA CGACCCTCGG CAGGGAGATA CCGTGAAACA TTACAAGATC 
    CGGACCCTGG ACAACGGGGG CTTCTACATA TCCCCCCGAA GCACCTTCAG CACTCTGCAG 
    GAGCTGGTGG ACCACTACAA GAAGGGGAAC GACGGGCTCT GCCAGAAACT GTCGGTGCCC 
    TGCATGTCTT CCAAGCCCCA GAAGCCTTGG GAGAAAGATG CCTGGGAGAT CCCTCGGGAA 
    TCCCTCAAGC TGGAGAAGAA ACTTGGAGCT GGGCAGTTTG GGGAAGTCTG GATGGCCACC 
    TACAACAAGC ACACCAAGGT GGCAGTGAAG ACGATGAAGC CAGGGAGCAT GTCGGTGGAG 
    GCCTTCCTGG CAGAGGCCAA CGTGATGAAA ACTCTGCAGC ATGACAAGCT GGTCAAACTT 
    CATGCGGTGG TCACCAAGGA GCCCATCTAC ATCATCACGG AGTTCATGGC CAAAGGAAGC 
    TTGCTGGACT TTCTGAAAAG TGATGAGGGC AGCAAGCAGC CATTGCCAAA ACTCATTGAC 
    TTCTCAGCCC AGATTGCAGA AGGCATGGCC TTCATCGAGC AGAGGAACTA CATCCACCGA 
    GACCTCCGAG CTGCCAACAT CTTGGTCTCT GCATCCCTGG TGTGTAAGAT TGCTGACTTT 
    GGCCTGGCCC GGGTCATTGA GGACAACGAG TACACGGCTC GGGAAGGGGC CAAGTTCCCC 
    ATCAAGTGGA CAGCTCCTGA AGCCATCAAC TTTGGCTCCT TCACCATCAA GTCAGACGTC 
    TGGTCCTTTG GTATCCTGCT GATGGAGATC GTCACCTACG GCCGGATCCC TTACCCAGGG 
    ATGTCAAACC CTGAAGTGAT CCGAGCTCTG GAGCGTGGAT ACCGGATGCC TCGCCCAGAG 
    AACTGCCCAG AGGAGCTCTA CAACATCATG ATGCGCTGCT GGAAAAACCG TCCGGAGGAG 
    CGGCCGACCT TCGAATACAT CCAGAGTGTG CTGGATGACT TCTACACGGC CACAGAGAGC 
    CAGTACCAAC AGCAGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002101.2
    CDS184..1764
    Translation

    Target ORF information:

    RefSeq Version NM_002110.4
    Organism Homo sapiens(human)
    Definition Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_002110.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    CTGGGGGGGC GCTCAAGCTG CGAGGATCCG GGCTGCCCGC GAGACGAGGA GCGGGCGCCC 
    AGGATGGGGT GCATGAAGTC CAAGTTCCTC CAGGTCGGAG GCAATACATT CTCAAAAACT 
    GAAACCAGCG CCAGCCCACA CTGTCCTGTG TACGTGCCGG ATCCCACATC CACCATCAAG 
    CCGGGGCCTA ATAGCCACAA CAGCAACACA CCAGGAATCA GGGAGGCAGG CTCTGAGGAC 
    ATCATCGTGG TTGCCCTGTA TGATTACGAG GCCATTCACC ACGAAGACCT CAGCTTCCAG 
    AAGGGGGACC AGATGGTGGT CCTAGAGGAA TCCGGGGAGT GGTGGAAGGC TCGATCCCTG 
    GCCACCCGGA AGGAGGGCTA CATCCCAAGC AACTATGTCG CCCGCGTTGA CTCTCTGGAG 
    ACAGAGGAGT GGTTTTTCAA GGGCATCAGC CGGAAGGACG CAGAGCGCCA ACTGCTGGCT 
    CCCGGCAACA TGCTGGGCTC CTTCATGATC CGGGATAGCG AGACCACTAA AGGAAGCTAC 
    TCTTTGTCCG TGCGAGACTA CGACCCTCGG CAGGGAGATA CCGTGAAACA TTACAAGATC 
    CGGACCCTGG ACAACGGGGG CTTCTACATA TCCCCCCGAA GCACCTTCAG CACTCTGCAG 
    GAGCTGGTGG ACCACTACAA GAAGGGGAAC GACGGGCTCT GCCAGAAACT GTCGGTGCCC 
    TGCATGTCTT CCAAGCCCCA GAAGCCTTGG GAGAAAGATG CCTGGGAGAT CCCTCGGGAA 
    TCCCTCAAGC TGGAGAAGAA ACTTGGAGCT GGGCAGTTTG GGGAAGTCTG GATGGCCACC 
    TACAACAAGC ACACCAAGGT GGCAGTGAAG ACGATGAAGC CAGGGAGCAT GTCGGTGGAG 
    GCCTTCCTGG CAGAGGCCAA CGTGATGAAA ACTCTGCAGC ATGACAAGCT GGTCAAACTT 
    CATGCGGTGG TCACCAAGGA GCCCATCTAC ATCATCACGG AGTTCATGGC CAAAGGAAGC 
    TTGCTGGACT TTCTGAAAAG TGATGAGGGC AGCAAGCAGC CATTGCCAAA ACTCATTGAC 
    TTCTCAGCCC AGATTGCAGA AGGCATGGCC TTCATCGAGC AGAGGAACTA CATCCACCGA 
    GACCTCCGAG CTGCCAACAT CTTGGTCTCT GCATCCCTGG TGTGTAAGAT TGCTGACTTT 
    GGCCTGGCCC GGGTCATTGA GGACAACGAG TACACGGCTC GGGAAGGGGC CAAGTTCCCC 
    ATCAAGTGGA CAGCTCCTGA AGCCATCAAC TTTGGCTCCT TCACCATCAA GTCAGACGTC 
    TGGTCCTTTG GTATCCTGCT GATGGAGATC GTCACCTACG GCCGGATCCC TTACCCAGGG 
    ATGTCAAACC CTGAAGTGAT CCGAGCTCTG GAGCGTGGAT ACCGGATGCC TCGCCCAGAG 
    AACTGCCCAG AGGAGCTCTA CAACATCATG ATGCGCTGCT GGAAAAACCG TCCGGAGGAG 
    CGGCCGACCT TCGAATACAT CCAGAGTGTG CTGGATGACT TCTACACGGC CACAGAGAGC 
    CAGTACCAAC AGCAGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    HCK ( NM_001172132.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165603.1 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OHu28510
    Clone ID Related Accession (Same CDS sequence) NM_001172132.2 , NM_001172132.1
    Accession Version NM_001172132.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Homo sapiens(human)
    Product tyrosine-protein kinase HCK isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290928.1. On Jun 2, 2019 this sequence version replaced NM_001172132.1. Transcript Variant: This variant (3) differs in its 5' UTR and uses an alternate AUG translation start codon, compared to variant 1. The encoded isoform (e) has a distinct and shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290928.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGATGGGGT GCATGAAGTC CAAGTTCCTC CAGGTCGGAG GCAATACATT CTCAAAAACT 
    GAAACCAGCG CCAGCCCACA CTGTCCTGTG TACGTGCCGG ATCCCACATC CACCATCAAG 
    CCGGGGCCTA ATAGCCACAA CAGCAACACA CCAGGAATCA GGGAGGCAGG CTCTGAGGAC 
    ATCATCGTGG TTGCCCTGTA TGATTACGAG GCCATTCACC ACGAAGACCT CAGCTTCCAG 
    AAGGGGGACC AGATGGTGGT CCTAGAGGAA TCCGGGGAGT GGTGGAAGGC TCGATCCCTG 
    GCCACCCGGA AGGAGGGCTA CATCCCAAGC AACTATGTCG CCCGCGTTGA CTCTCTGGAG 
    ACAGAGGAGT GGTTTTTCAA GGGCATCAGC CGGAAGGACG CAGAGCGCCA ACTGCTGGCT 
    CCCGGCAACA TGCTGGGCTC CTTCATGATC CGGGATAGCG AGACCACTAA AGGAAGCTAC 
    TCTTTGTCCG TGCGAGACTA CGACCCTCGG CAGGGAGATA CCGTGAAACA TTACAAGATC 
    CGGACCCTGG ACAACGGGGG CTTCTACATA TCCCCCCGAA GCACCTTCAG CACTCTGCAG 
    GAGCTGGTGG ACCACTACAA GAAGGGGAAC GACGGGCTCT GCCAGAAACT GTCGGTGCCC 
    TGCATGTCTT CCAAGCCCCA GAAGCCTTGG GAGAAAGATG CCTGGGAGAT CCCTCGGGAA 
    TCCCTCAAGC TGGAGAAGAA ACTTGGAGCT GGGCAGTTTG GGGAAGTCTG GATGGCCACC 
    TACAACAAGC ACACCAAGGT GGCAGTGAAG ACGATGAAGC CAGGGAGCAT GTCGGTGGAG 
    GCCTTCCTGG CAGAGGCCAA CGTGATGAAA ACTCTGCAGC ATGACAAGCT GGTCAAACTT 
    CATGCGGTGG TCACCAAGGA GCCCATCTAC ATCATCACGG AGTTCATGGC CAAAGGAAGC 
    TTGCTGGACT TTCTGAAAAG TGATGAGGGC AGCAAGCAGC CATTGCCAAA ACTCATTGAC 
    TTCTCAGCCC AGATTGCAGA AGGCATGGCC TTCATCGAGC AGAGGAACTA CATCCACCGA 
    GACCTCCGAG CTGCCAACAT CTTGGTCTCT GCATCCCTGG TGTGTAAGAT TGCTGACTTT 
    GGCCTGGCCC GGGTCATTGA GGACAACGAG TACACGGCTC GGGAAGGGGC CAAGTTCCCC 
    ATCAAGTGGA CAGCTCCTGA AGCCATCAAC TTTGGCTCCT TCACCATCAA GTCAGACGTC 
    TGGTCCTTTG GTATCCTGCT GATGGAGATC GTCACCTACG GCCGGATCCC TTACCCAGGG 
    ATGTCAAACC CTGAAGTGAT CCGAGCTCTG GAGCGTGGAT ACCGGATGCC TCGCCCAGAG 
    AACTGCCCAG AGGAGCTCTA CAACATCATG ATGCGCTGCT GGAAAAACCG TCCGGAGGAG 
    CGGCCGACCT TCGAATACAT CCAGAGTGTG CTGGATGACT TCTACACGGC CACAGAGAGC 
    CAGTACCAAC AGCAGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165603.1
    CDS310..1830
    Translation

    Target ORF information:

    RefSeq Version NM_001172132.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001172132.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGATGGGGT GCATGAAGTC CAAGTTCCTC CAGGTCGGAG GCAATACATT CTCAAAAACT 
    GAAACCAGCG CCAGCCCACA CTGTCCTGTG TACGTGCCGG ATCCCACATC CACCATCAAG 
    CCGGGGCCTA ATAGCCACAA CAGCAACACA CCAGGAATCA GGGAGGCAGG CTCTGAGGAC 
    ATCATCGTGG TTGCCCTGTA TGATTACGAG GCCATTCACC ACGAAGACCT CAGCTTCCAG 
    AAGGGGGACC AGATGGTGGT CCTAGAGGAA TCCGGGGAGT GGTGGAAGGC TCGATCCCTG 
    GCCACCCGGA AGGAGGGCTA CATCCCAAGC AACTATGTCG CCCGCGTTGA CTCTCTGGAG 
    ACAGAGGAGT GGTTTTTCAA GGGCATCAGC CGGAAGGACG CAGAGCGCCA ACTGCTGGCT 
    CCCGGCAACA TGCTGGGCTC CTTCATGATC CGGGATAGCG AGACCACTAA AGGAAGCTAC 
    TCTTTGTCCG TGCGAGACTA CGACCCTCGG CAGGGAGATA CCGTGAAACA TTACAAGATC 
    CGGACCCTGG ACAACGGGGG CTTCTACATA TCCCCCCGAA GCACCTTCAG CACTCTGCAG 
    GAGCTGGTGG ACCACTACAA GAAGGGGAAC GACGGGCTCT GCCAGAAACT GTCGGTGCCC 
    TGCATGTCTT CCAAGCCCCA GAAGCCTTGG GAGAAAGATG CCTGGGAGAT CCCTCGGGAA 
    TCCCTCAAGC TGGAGAAGAA ACTTGGAGCT GGGCAGTTTG GGGAAGTCTG GATGGCCACC 
    TACAACAAGC ACACCAAGGT GGCAGTGAAG ACGATGAAGC CAGGGAGCAT GTCGGTGGAG 
    GCCTTCCTGG CAGAGGCCAA CGTGATGAAA ACTCTGCAGC ATGACAAGCT GGTCAAACTT 
    CATGCGGTGG TCACCAAGGA GCCCATCTAC ATCATCACGG AGTTCATGGC CAAAGGAAGC 
    TTGCTGGACT TTCTGAAAAG TGATGAGGGC AGCAAGCAGC CATTGCCAAA ACTCATTGAC 
    TTCTCAGCCC AGATTGCAGA AGGCATGGCC TTCATCGAGC AGAGGAACTA CATCCACCGA 
    GACCTCCGAG CTGCCAACAT CTTGGTCTCT GCATCCCTGG TGTGTAAGAT TGCTGACTTT 
    GGCCTGGCCC GGGTCATTGA GGACAACGAG TACACGGCTC GGGAAGGGGC CAAGTTCCCC 
    ATCAAGTGGA CAGCTCCTGA AGCCATCAAC TTTGGCTCCT TCACCATCAA GTCAGACGTC 
    TGGTCCTTTG GTATCCTGCT GATGGAGATC GTCACCTACG GCCGGATCCC TTACCCAGGG 
    ATGTCAAACC CTGAAGTGAT CCGAGCTCTG GAGCGTGGAT ACCGGATGCC TCGCCCAGAG 
    AACTGCCCAG AGGAGCTCTA CAACATCATG ATGCGCTGCT GGAAAAACCG TCCGGAGGAG 
    CGGCCGACCT TCGAATACAT CCAGAGTGTG CTGGATGACT TCTACACGGC CACAGAGAGC 
    CAGTACCAAC AGCAGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    HCK ( NM_001172132.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165603.1 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OHu28510
    Clone ID Related Accession (Same CDS sequence) NM_001172132.2 , NM_001172132.1
    Accession Version NM_001172132.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product tyrosine-protein kinase HCK isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA465978.1, AK290928.1 and AW139272.1. Transcript Variant: This variant (3) differs in its 5' UTR and uses an alternate AUG translation start codon, compared to variant 1. The encoded isoform (e) has a distinct and shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290928.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGATGGGGT GCATGAAGTC CAAGTTCCTC CAGGTCGGAG GCAATACATT CTCAAAAACT 
    GAAACCAGCG CCAGCCCACA CTGTCCTGTG TACGTGCCGG ATCCCACATC CACCATCAAG 
    CCGGGGCCTA ATAGCCACAA CAGCAACACA CCAGGAATCA GGGAGGCAGG CTCTGAGGAC 
    ATCATCGTGG TTGCCCTGTA TGATTACGAG GCCATTCACC ACGAAGACCT CAGCTTCCAG 
    AAGGGGGACC AGATGGTGGT CCTAGAGGAA TCCGGGGAGT GGTGGAAGGC TCGATCCCTG 
    GCCACCCGGA AGGAGGGCTA CATCCCAAGC AACTATGTCG CCCGCGTTGA CTCTCTGGAG 
    ACAGAGGAGT GGTTTTTCAA GGGCATCAGC CGGAAGGACG CAGAGCGCCA ACTGCTGGCT 
    CCCGGCAACA TGCTGGGCTC CTTCATGATC CGGGATAGCG AGACCACTAA AGGAAGCTAC 
    TCTTTGTCCG TGCGAGACTA CGACCCTCGG CAGGGAGATA CCGTGAAACA TTACAAGATC 
    CGGACCCTGG ACAACGGGGG CTTCTACATA TCCCCCCGAA GCACCTTCAG CACTCTGCAG 
    GAGCTGGTGG ACCACTACAA GAAGGGGAAC GACGGGCTCT GCCAGAAACT GTCGGTGCCC 
    TGCATGTCTT CCAAGCCCCA GAAGCCTTGG GAGAAAGATG CCTGGGAGAT CCCTCGGGAA 
    TCCCTCAAGC TGGAGAAGAA ACTTGGAGCT GGGCAGTTTG GGGAAGTCTG GATGGCCACC 
    TACAACAAGC ACACCAAGGT GGCAGTGAAG ACGATGAAGC CAGGGAGCAT GTCGGTGGAG 
    GCCTTCCTGG CAGAGGCCAA CGTGATGAAA ACTCTGCAGC ATGACAAGCT GGTCAAACTT 
    CATGCGGTGG TCACCAAGGA GCCCATCTAC ATCATCACGG AGTTCATGGC CAAAGGAAGC 
    TTGCTGGACT TTCTGAAAAG TGATGAGGGC AGCAAGCAGC CATTGCCAAA ACTCATTGAC 
    TTCTCAGCCC AGATTGCAGA AGGCATGGCC TTCATCGAGC AGAGGAACTA CATCCACCGA 
    GACCTCCGAG CTGCCAACAT CTTGGTCTCT GCATCCCTGG TGTGTAAGAT TGCTGACTTT 
    GGCCTGGCCC GGGTCATTGA GGACAACGAG TACACGGCTC GGGAAGGGGC CAAGTTCCCC 
    ATCAAGTGGA CAGCTCCTGA AGCCATCAAC TTTGGCTCCT TCACCATCAA GTCAGACGTC 
    TGGTCCTTTG GTATCCTGCT GATGGAGATC GTCACCTACG GCCGGATCCC TTACCCAGGG 
    ATGTCAAACC CTGAAGTGAT CCGAGCTCTG GAGCGTGGAT ACCGGATGCC TCGCCCAGAG 
    AACTGCCCAG AGGAGCTCTA CAACATCATG ATGCGCTGCT GGAAAAACCG TCCGGAGGAG 
    CGGCCGACCT TCGAATACAT CCAGAGTGTG CTGGATGACT TCTACACGGC CACAGAGAGC 
    CAGTACCAAC AGCAGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165603.1
    CDS364..1884
    Translation

    Target ORF information:

    RefSeq Version NM_001172132.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001172132.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGATGGGGT GCATGAAGTC CAAGTTCCTC CAGGTCGGAG GCAATACATT CTCAAAAACT 
    GAAACCAGCG CCAGCCCACA CTGTCCTGTG TACGTGCCGG ATCCCACATC CACCATCAAG 
    CCGGGGCCTA ATAGCCACAA CAGCAACACA CCAGGAATCA GGGAGGCAGG CTCTGAGGAC 
    ATCATCGTGG TTGCCCTGTA TGATTACGAG GCCATTCACC ACGAAGACCT CAGCTTCCAG 
    AAGGGGGACC AGATGGTGGT CCTAGAGGAA TCCGGGGAGT GGTGGAAGGC TCGATCCCTG 
    GCCACCCGGA AGGAGGGCTA CATCCCAAGC AACTATGTCG CCCGCGTTGA CTCTCTGGAG 
    ACAGAGGAGT GGTTTTTCAA GGGCATCAGC CGGAAGGACG CAGAGCGCCA ACTGCTGGCT 
    CCCGGCAACA TGCTGGGCTC CTTCATGATC CGGGATAGCG AGACCACTAA AGGAAGCTAC 
    TCTTTGTCCG TGCGAGACTA CGACCCTCGG CAGGGAGATA CCGTGAAACA TTACAAGATC 
    CGGACCCTGG ACAACGGGGG CTTCTACATA TCCCCCCGAA GCACCTTCAG CACTCTGCAG 
    GAGCTGGTGG ACCACTACAA GAAGGGGAAC GACGGGCTCT GCCAGAAACT GTCGGTGCCC 
    TGCATGTCTT CCAAGCCCCA GAAGCCTTGG GAGAAAGATG CCTGGGAGAT CCCTCGGGAA 
    TCCCTCAAGC TGGAGAAGAA ACTTGGAGCT GGGCAGTTTG GGGAAGTCTG GATGGCCACC 
    TACAACAAGC ACACCAAGGT GGCAGTGAAG ACGATGAAGC CAGGGAGCAT GTCGGTGGAG 
    GCCTTCCTGG CAGAGGCCAA CGTGATGAAA ACTCTGCAGC ATGACAAGCT GGTCAAACTT 
    CATGCGGTGG TCACCAAGGA GCCCATCTAC ATCATCACGG AGTTCATGGC CAAAGGAAGC 
    TTGCTGGACT TTCTGAAAAG TGATGAGGGC AGCAAGCAGC CATTGCCAAA ACTCATTGAC 
    TTCTCAGCCC AGATTGCAGA AGGCATGGCC TTCATCGAGC AGAGGAACTA CATCCACCGA 
    GACCTCCGAG CTGCCAACAT CTTGGTCTCT GCATCCCTGG TGTGTAAGAT TGCTGACTTT 
    GGCCTGGCCC GGGTCATTGA GGACAACGAG TACACGGCTC GGGAAGGGGC CAAGTTCCCC 
    ATCAAGTGGA CAGCTCCTGA AGCCATCAAC TTTGGCTCCT TCACCATCAA GTCAGACGTC 
    TGGTCCTTTG GTATCCTGCT GATGGAGATC GTCACCTACG GCCGGATCCC TTACCCAGGG 
    ATGTCAAACC CTGAAGTGAT CCGAGCTCTG GAGCGTGGAT ACCGGATGCC TCGCCCAGAG 
    AACTGCCCAG AGGAGCTCTA CAACATCATG ATGCGCTGCT GGAAAAACCG TCCGGAGGAG 
    CGGCCGACCT TCGAATACAT CCAGAGTGTG CTGGATGACT TCTACACGGC CACAGAGAGC 
    CAGTACCAAC AGCAGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    HCK ( NM_001172131.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165602.1 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu28528
    Clone ID Related Accession (Same CDS sequence) NM_001172131.2 , NM_001172131.1
    Accession Version NM_001172131.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1515bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Homo sapiens(human)
    Product tyrosine-protein kinase HCK isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA465978.1, BC113854.1, AK289896.1 and AW139272.1. On Jun 2, 2019 this sequence version replaced NM_001172131.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This variant encodes two isoforms due to the use of alternative translation initiation codons, as demonstrated in PMIDs 1875927 and 7791757. The longer isoform (c) is derived from an upstream non-AUG (CUG) start codon, while the shorter isoform (d) is derived from a downstream AUG start codon. The shorter isoform (d) is represented in this RefSeq, and is overall shorter, compared to isoform a. CCDS Note: This CCDS, which is supported by the mRNAs AK289896.1 and BC113854.1, represents a short human HCK isoform, as described in PMID:7791757. This isoform initiates translation from a downstream AUG start codon. Alternative translation initiation from an upstream non-AUG (CUG) start codon, which is well-conserved and present in a strong Kozak signal context, produces an isoform that is 21 aa longer at the N-terminus. The longer isoform encoded by this variant is represented by CCDS 54453.1. These isoforms exhibit distinct subcellular localization, as indicated in PMID:7791757. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.76670.1, SRR7410570.662727.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:7791757) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGGTGCA TGAAGTCCAA GTTCCTCCAG GTCGGAGGCA ATACATTCTC AAAAACTGAA 
    ACCAGCGCCA GCCCACACTG TCCTGTGTAC GTGCCGGATC CCACATCCAC CATCAAGCCG 
    GGGCCTAATA GCCACAACAG CAACACACCA GGAATCAGGG AGGGCTCTGA GGACATCATC 
    GTGGTTGCCC TGTATGATTA CGAGGCCATT CACCACGAAG ACCTCAGCTT CCAGAAGGGG 
    GACCAGATGG TGGTCCTAGA GGAATCCGGG GAGTGGTGGA AGGCTCGATC CCTGGCCACC 
    CGGAAGGAGG GCTACATCCC AAGCAACTAT GTCGCCCGCG TTGACTCTCT GGAGACAGAG 
    GAGTGGTTTT TCAAGGGCAT CAGCCGGAAG GACGCAGAGC GCCAACTGCT GGCTCCCGGC 
    AACATGCTGG GCTCCTTCAT GATCCGGGAT AGCGAGACCA CTAAAGGAAG CTACTCTTTG 
    TCCGTGCGAG ACTACGACCC TCGGCAGGGA GATACCGTGA AACATTACAA GATCCGGACC 
    CTGGACAACG GGGGCTTCTA CATATCCCCC CGAAGCACCT TCAGCACTCT GCAGGAGCTG 
    GTGGACCACT ACAAGAAGGG GAACGACGGG CTCTGCCAGA AACTGTCGGT GCCCTGCATG 
    TCTTCCAAGC CCCAGAAGCC TTGGGAGAAA GATGCCTGGG AGATCCCTCG GGAATCCCTC 
    AAGCTGGAGA AGAAACTTGG AGCTGGGCAG TTTGGGGAAG TCTGGATGGC CACCTACAAC 
    AAGCACACCA AGGTGGCAGT GAAGACGATG AAGCCAGGGA GCATGTCGGT GGAGGCCTTC 
    CTGGCAGAGG CCAACGTGAT GAAAACTCTG CAGCATGACA AGCTGGTCAA ACTTCATGCG 
    GTGGTCACCA AGGAGCCCAT CTACATCATC ACGGAGTTCA TGGCCAAAGG AAGCTTGCTG 
    GACTTTCTGA AAAGTGATGA GGGCAGCAAG CAGCCATTGC CAAAACTCAT TGACTTCTCA 
    GCCCAGATTG CAGAAGGCAT GGCCTTCATC GAGCAGAGGA ACTACATCCA CCGAGACCTC 
    CGAGCTGCCA ACATCTTGGT CTCTGCATCC CTGGTGTGTA AGATTGCTGA CTTTGGCCTG 
    GCCCGGGTCA TTGAGGACAA CGAGTACACG GCTCGGGAAG GGGCCAAGTT CCCCATCAAG 
    TGGACAGCTC CTGAAGCCAT CAACTTTGGC TCCTTCACCA TCAAGTCAGA CGTCTGGTCC 
    TTTGGTATCC TGCTGATGGA GATCGTCACC TACGGCCGGA TCCCTTACCC AGGGATGTCA 
    AACCCTGAAG TGATCCGAGC TCTGGAGCGT GGATACCGGA TGCCTCGCCC AGAGAACTGC 
    CCAGAGGAGC TCTACAACAT CATGATGCGC TGCTGGAAAA ACCGTCCGGA GGAGCGGCCG 
    ACCTTCGAAT ACATCCAGAG TGTGCTGGAT GACTTCTACA CGGCCACAGA GAGCCAGTAC 
    CAACAGCAGC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165602.1
    CDS247..1761
    Translation

    Target ORF information:

    RefSeq Version NM_001172131.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001172131.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGGTGCA TGAAGTCCAA GTTCCTCCAG GTCGGAGGCA ATACATTCTC AAAAACTGAA 
    ACCAGCGCCA GCCCACACTG TCCTGTGTAC GTGCCGGATC CCACATCCAC CATCAAGCCG 
    GGGCCTAATA GCCACAACAG CAACACACCA GGAATCAGGG AGGGCTCTGA GGACATCATC 
    GTGGTTGCCC TGTATGATTA CGAGGCCATT CACCACGAAG ACCTCAGCTT CCAGAAGGGG 
    GACCAGATGG TGGTCCTAGA GGAATCCGGG GAGTGGTGGA AGGCTCGATC CCTGGCCACC 
    CGGAAGGAGG GCTACATCCC AAGCAACTAT GTCGCCCGCG TTGACTCTCT GGAGACAGAG 
    GAGTGGTTTT TCAAGGGCAT CAGCCGGAAG GACGCAGAGC GCCAACTGCT GGCTCCCGGC 
    AACATGCTGG GCTCCTTCAT GATCCGGGAT AGCGAGACCA CTAAAGGAAG CTACTCTTTG 
    TCCGTGCGAG ACTACGACCC TCGGCAGGGA GATACCGTGA AACATTACAA GATCCGGACC 
    CTGGACAACG GGGGCTTCTA CATATCCCCC CGAAGCACCT TCAGCACTCT GCAGGAGCTG 
    GTGGACCACT ACAAGAAGGG GAACGACGGG CTCTGCCAGA AACTGTCGGT GCCCTGCATG 
    TCTTCCAAGC CCCAGAAGCC TTGGGAGAAA GATGCCTGGG AGATCCCTCG GGAATCCCTC 
    AAGCTGGAGA AGAAACTTGG AGCTGGGCAG TTTGGGGAAG TCTGGATGGC CACCTACAAC 
    AAGCACACCA AGGTGGCAGT GAAGACGATG AAGCCAGGGA GCATGTCGGT GGAGGCCTTC 
    CTGGCAGAGG CCAACGTGAT GAAAACTCTG CAGCATGACA AGCTGGTCAA ACTTCATGCG 
    GTGGTCACCA AGGAGCCCAT CTACATCATC ACGGAGTTCA TGGCCAAAGG AAGCTTGCTG 
    GACTTTCTGA AAAGTGATGA GGGCAGCAAG CAGCCATTGC CAAAACTCAT TGACTTCTCA 
    GCCCAGATTG CAGAAGGCAT GGCCTTCATC GAGCAGAGGA ACTACATCCA CCGAGACCTC 
    CGAGCTGCCA ACATCTTGGT CTCTGCATCC CTGGTGTGTA AGATTGCTGA CTTTGGCCTG 
    GCCCGGGTCA TTGAGGACAA CGAGTACACG GCTCGGGAAG GGGCCAAGTT CCCCATCAAG 
    TGGACAGCTC CTGAAGCCAT CAACTTTGGC TCCTTCACCA TCAAGTCAGA CGTCTGGTCC 
    TTTGGTATCC TGCTGATGGA GATCGTCACC TACGGCCGGA TCCCTTACCC AGGGATGTCA 
    AACCCTGAAG TGATCCGAGC TCTGGAGCGT GGATACCGGA TGCCTCGCCC AGAGAACTGC 
    CCAGAGGAGC TCTACAACAT CATGATGCGC TGCTGGAAAA ACCGTCCGGA GGAGCGGCCG 
    ACCTTCGAAT ACATCCAGAG TGTGCTGGAT GACTTCTACA CGGCCACAGA GAGCCAGTAC 
    CAACAGCAGC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    HCK ( NM_001172131.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165602.1 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu28528
    Clone ID Related Accession (Same CDS sequence) NM_001172131.2 , NM_001172131.1
    Accession Version NM_001172131.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1515bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product tyrosine-protein kinase HCK isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA465978.1, BC113854.1, AK289896.1 and AW139272.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This variant encodes two isoforms due to the use of alternative translation initiation codons, as demonstrated in PMIDs 1875927 and 7791757. The longer isoform (c) is derived from an upstream non-AUG (CUG) start codon, while the shorter isoform (d) is derived from a downstream AUG start codon. The shorter isoform (d) is represented in this RefSeq, and is overall shorter, compared to isoform a. CCDS Note: This CCDS, which is supported by the mRNAs AK289896.1 and BC113854.1, represents a short human HCK isoform, as described in PMID:7791757. This isoform initiates translation from a downstream AUG start codon. Alternative translation initiation from an upstream non-AUG (CUG) start codon, which is well-conserved and present in a strong Kozak signal context, produces an isoform that is 21 aa longer at the N-terminus. The longer isoform encoded by this variant is represented by CCDS 54453.1. These isoforms exhibit distinct subcellular localization, as indicated in PMID:7791757. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.76670.1, SRR1803615.185351.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:7791757) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGGTGCA TGAAGTCCAA GTTCCTCCAG GTCGGAGGCA ATACATTCTC AAAAACTGAA 
    ACCAGCGCCA GCCCACACTG TCCTGTGTAC GTGCCGGATC CCACATCCAC CATCAAGCCG 
    GGGCCTAATA GCCACAACAG CAACACACCA GGAATCAGGG AGGGCTCTGA GGACATCATC 
    GTGGTTGCCC TGTATGATTA CGAGGCCATT CACCACGAAG ACCTCAGCTT CCAGAAGGGG 
    GACCAGATGG TGGTCCTAGA GGAATCCGGG GAGTGGTGGA AGGCTCGATC CCTGGCCACC 
    CGGAAGGAGG GCTACATCCC AAGCAACTAT GTCGCCCGCG TTGACTCTCT GGAGACAGAG 
    GAGTGGTTTT TCAAGGGCAT CAGCCGGAAG GACGCAGAGC GCCAACTGCT GGCTCCCGGC 
    AACATGCTGG GCTCCTTCAT GATCCGGGAT AGCGAGACCA CTAAAGGAAG CTACTCTTTG 
    TCCGTGCGAG ACTACGACCC TCGGCAGGGA GATACCGTGA AACATTACAA GATCCGGACC 
    CTGGACAACG GGGGCTTCTA CATATCCCCC CGAAGCACCT TCAGCACTCT GCAGGAGCTG 
    GTGGACCACT ACAAGAAGGG GAACGACGGG CTCTGCCAGA AACTGTCGGT GCCCTGCATG 
    TCTTCCAAGC CCCAGAAGCC TTGGGAGAAA GATGCCTGGG AGATCCCTCG GGAATCCCTC 
    AAGCTGGAGA AGAAACTTGG AGCTGGGCAG TTTGGGGAAG TCTGGATGGC CACCTACAAC 
    AAGCACACCA AGGTGGCAGT GAAGACGATG AAGCCAGGGA GCATGTCGGT GGAGGCCTTC 
    CTGGCAGAGG CCAACGTGAT GAAAACTCTG CAGCATGACA AGCTGGTCAA ACTTCATGCG 
    GTGGTCACCA AGGAGCCCAT CTACATCATC ACGGAGTTCA TGGCCAAAGG AAGCTTGCTG 
    GACTTTCTGA AAAGTGATGA GGGCAGCAAG CAGCCATTGC CAAAACTCAT TGACTTCTCA 
    GCCCAGATTG CAGAAGGCAT GGCCTTCATC GAGCAGAGGA ACTACATCCA CCGAGACCTC 
    CGAGCTGCCA ACATCTTGGT CTCTGCATCC CTGGTGTGTA AGATTGCTGA CTTTGGCCTG 
    GCCCGGGTCA TTGAGGACAA CGAGTACACG GCTCGGGAAG GGGCCAAGTT CCCCATCAAG 
    TGGACAGCTC CTGAAGCCAT CAACTTTGGC TCCTTCACCA TCAAGTCAGA CGTCTGGTCC 
    TTTGGTATCC TGCTGATGGA GATCGTCACC TACGGCCGGA TCCCTTACCC AGGGATGTCA 
    AACCCTGAAG TGATCCGAGC TCTGGAGCGT GGATACCGGA TGCCTCGCCC AGAGAACTGC 
    CCAGAGGAGC TCTACAACAT CATGATGCGC TGCTGGAAAA ACCGTCCGGA GGAGCGGCCG 
    ACCTTCGAAT ACATCCAGAG TGTGCTGGAT GACTTCTACA CGGCCACAGA GAGCCAGTAC 
    CAACAGCAGC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165602.1
    CDS301..1815
    Translation

    Target ORF information:

    RefSeq Version NM_001172131.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001172131.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGGTGCA TGAAGTCCAA GTTCCTCCAG GTCGGAGGCA ATACATTCTC AAAAACTGAA 
    ACCAGCGCCA GCCCACACTG TCCTGTGTAC GTGCCGGATC CCACATCCAC CATCAAGCCG 
    GGGCCTAATA GCCACAACAG CAACACACCA GGAATCAGGG AGGGCTCTGA GGACATCATC 
    GTGGTTGCCC TGTATGATTA CGAGGCCATT CACCACGAAG ACCTCAGCTT CCAGAAGGGG 
    GACCAGATGG TGGTCCTAGA GGAATCCGGG GAGTGGTGGA AGGCTCGATC CCTGGCCACC 
    CGGAAGGAGG GCTACATCCC AAGCAACTAT GTCGCCCGCG TTGACTCTCT GGAGACAGAG 
    GAGTGGTTTT TCAAGGGCAT CAGCCGGAAG GACGCAGAGC GCCAACTGCT GGCTCCCGGC 
    AACATGCTGG GCTCCTTCAT GATCCGGGAT AGCGAGACCA CTAAAGGAAG CTACTCTTTG 
    TCCGTGCGAG ACTACGACCC TCGGCAGGGA GATACCGTGA AACATTACAA GATCCGGACC 
    CTGGACAACG GGGGCTTCTA CATATCCCCC CGAAGCACCT TCAGCACTCT GCAGGAGCTG 
    GTGGACCACT ACAAGAAGGG GAACGACGGG CTCTGCCAGA AACTGTCGGT GCCCTGCATG 
    TCTTCCAAGC CCCAGAAGCC TTGGGAGAAA GATGCCTGGG AGATCCCTCG GGAATCCCTC 
    AAGCTGGAGA AGAAACTTGG AGCTGGGCAG TTTGGGGAAG TCTGGATGGC CACCTACAAC 
    AAGCACACCA AGGTGGCAGT GAAGACGATG AAGCCAGGGA GCATGTCGGT GGAGGCCTTC 
    CTGGCAGAGG CCAACGTGAT GAAAACTCTG CAGCATGACA AGCTGGTCAA ACTTCATGCG 
    GTGGTCACCA AGGAGCCCAT CTACATCATC ACGGAGTTCA TGGCCAAAGG AAGCTTGCTG 
    GACTTTCTGA AAAGTGATGA GGGCAGCAAG CAGCCATTGC CAAAACTCAT TGACTTCTCA 
    GCCCAGATTG CAGAAGGCAT GGCCTTCATC GAGCAGAGGA ACTACATCCA CCGAGACCTC 
    CGAGCTGCCA ACATCTTGGT CTCTGCATCC CTGGTGTGTA AGATTGCTGA CTTTGGCCTG 
    GCCCGGGTCA TTGAGGACAA CGAGTACACG GCTCGGGAAG GGGCCAAGTT CCCCATCAAG 
    TGGACAGCTC CTGAAGCCAT CAACTTTGGC TCCTTCACCA TCAAGTCAGA CGTCTGGTCC 
    TTTGGTATCC TGCTGATGGA GATCGTCACC TACGGCCGGA TCCCTTACCC AGGGATGTCA 
    AACCCTGAAG TGATCCGAGC TCTGGAGCGT GGATACCGGA TGCCTCGCCC AGAGAACTGC 
    CCAGAGGAGC TCTACAACAT CATGATGCGC TGCTGGAAAA ACCGTCCGGA GGAGCGGCCG 
    ACCTTCGAAT ACATCCAGAG TGTGCTGGAT GACTTCTACA CGGCCACAGA GAGCCAGTAC 
    CAACAGCAGC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    HCK ( NM_001172130.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165601.1 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu28541
    Clone ID Related Accession (Same CDS sequence) NM_001172130.1 , NM_001172130.2
    Accession Version NM_001172130.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1578bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product tyrosine-protein kinase HCK isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA465978.1, BC113854.1, AK289896.1 and AW139272.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This variant encodes two isoforms due to the use of alternative translation initiation codons, as demonstrated in PMIDs 1875927 and 7791757. The longer isoform (c) is derived from an upstream non-AUG (CUG) start codon, while the shorter isoform (d) is derived from a downstream AUG start codon. The longer isoform (c) is represented in this RefSeq, but it is overall shorter, compared to isoform a. CCDS Note: This CCDS, which is supported by the mRNAs AK289896.1 and BC113854.1, represents a long human HCK isoform, as described in PMIDs 1875927 and 7791757. This isoform initiates translation from a non-AUG (CUG) start codon that is well-conserved and present in a strong Kozak signal context. Alternative translation initiation from a downstream AUG start codon produces an isoform that is 21 aa shorter at the N-terminus. The shorter isoform encoded by this variant is represented by CCDS 54456.1. These isoforms exhibit distinct subcellular distributions, as indicated in PMID:7791757. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.76670.1, SRR1803615.185351.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 7791757, 1875927 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    CTGGGGGGGC GCTCAAGCTG CGAGGATCCG GGCTGCCCGC GAGACGAGGA GCGGGCGCCC 
    AGGATGGGGT GCATGAAGTC CAAGTTCCTC CAGGTCGGAG GCAATACATT CTCAAAAACT 
    GAAACCAGCG CCAGCCCACA CTGTCCTGTG TACGTGCCGG ATCCCACATC CACCATCAAG 
    CCGGGGCCTA ATAGCCACAA CAGCAACACA CCAGGAATCA GGGAGGGCTC TGAGGACATC 
    ATCGTGGTTG CCCTGTATGA TTACGAGGCC ATTCACCACG AAGACCTCAG CTTCCAGAAG 
    GGGGACCAGA TGGTGGTCCT AGAGGAATCC GGGGAGTGGT GGAAGGCTCG ATCCCTGGCC 
    ACCCGGAAGG AGGGCTACAT CCCAAGCAAC TATGTCGCCC GCGTTGACTC TCTGGAGACA 
    GAGGAGTGGT TTTTCAAGGG CATCAGCCGG AAGGACGCAG AGCGCCAACT GCTGGCTCCC 
    GGCAACATGC TGGGCTCCTT CATGATCCGG GATAGCGAGA CCACTAAAGG AAGCTACTCT 
    TTGTCCGTGC GAGACTACGA CCCTCGGCAG GGAGATACCG TGAAACATTA CAAGATCCGG 
    ACCCTGGACA ACGGGGGCTT CTACATATCC CCCCGAAGCA CCTTCAGCAC TCTGCAGGAG 
    CTGGTGGACC ACTACAAGAA GGGGAACGAC GGGCTCTGCC AGAAACTGTC GGTGCCCTGC 
    ATGTCTTCCA AGCCCCAGAA GCCTTGGGAG AAAGATGCCT GGGAGATCCC TCGGGAATCC 
    CTCAAGCTGG AGAAGAAACT TGGAGCTGGG CAGTTTGGGG AAGTCTGGAT GGCCACCTAC 
    AACAAGCACA CCAAGGTGGC AGTGAAGACG ATGAAGCCAG GGAGCATGTC GGTGGAGGCC 
    TTCCTGGCAG AGGCCAACGT GATGAAAACT CTGCAGCATG ACAAGCTGGT CAAACTTCAT 
    GCGGTGGTCA CCAAGGAGCC CATCTACATC ATCACGGAGT TCATGGCCAA AGGAAGCTTG 
    CTGGACTTTC TGAAAAGTGA TGAGGGCAGC AAGCAGCCAT TGCCAAAACT CATTGACTTC 
    TCAGCCCAGA TTGCAGAAGG CATGGCCTTC ATCGAGCAGA GGAACTACAT CCACCGAGAC 
    CTCCGAGCTG CCAACATCTT GGTCTCTGCA TCCCTGGTGT GTAAGATTGC TGACTTTGGC 
    CTGGCCCGGG TCATTGAGGA CAACGAGTAC ACGGCTCGGG AAGGGGCCAA GTTCCCCATC 
    AAGTGGACAG CTCCTGAAGC CATCAACTTT GGCTCCTTCA CCATCAAGTC AGACGTCTGG 
    TCCTTTGGTA TCCTGCTGAT GGAGATCGTC ACCTACGGCC GGATCCCTTA CCCAGGGATG 
    TCAAACCCTG AAGTGATCCG AGCTCTGGAG CGTGGATACC GGATGCCTCG CCCAGAGAAC 
    TGCCCAGAGG AGCTCTACAA CATCATGATG CGCTGCTGGA AAAACCGTCC GGAGGAGCGG 
    CCGACCTTCG AATACATCCA GAGTGTGCTG GATGACTTCT ACACGGCCAC AGAGAGCCAG 
    TACCAACAGC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165601.1
    CDS238..1815
    Translation

    Target ORF information:

    RefSeq Version NM_001172130.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001172130.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    CTGGGGGGGC GCTCAAGCTG CGAGGATCCG GGCTGCCCGC GAGACGAGGA GCGGGCGCCC 
    AGGATGGGGT GCATGAAGTC CAAGTTCCTC CAGGTCGGAG GCAATACATT CTCAAAAACT 
    GAAACCAGCG CCAGCCCACA CTGTCCTGTG TACGTGCCGG ATCCCACATC CACCATCAAG 
    CCGGGGCCTA ATAGCCACAA CAGCAACACA CCAGGAATCA GGGAGGGCTC TGAGGACATC 
    ATCGTGGTTG CCCTGTATGA TTACGAGGCC ATTCACCACG AAGACCTCAG CTTCCAGAAG 
    GGGGACCAGA TGGTGGTCCT AGAGGAATCC GGGGAGTGGT GGAAGGCTCG ATCCCTGGCC 
    ACCCGGAAGG AGGGCTACAT CCCAAGCAAC TATGTCGCCC GCGTTGACTC TCTGGAGACA 
    GAGGAGTGGT TTTTCAAGGG CATCAGCCGG AAGGACGCAG AGCGCCAACT GCTGGCTCCC 
    GGCAACATGC TGGGCTCCTT CATGATCCGG GATAGCGAGA CCACTAAAGG AAGCTACTCT 
    TTGTCCGTGC GAGACTACGA CCCTCGGCAG GGAGATACCG TGAAACATTA CAAGATCCGG 
    ACCCTGGACA ACGGGGGCTT CTACATATCC CCCCGAAGCA CCTTCAGCAC TCTGCAGGAG 
    CTGGTGGACC ACTACAAGAA GGGGAACGAC GGGCTCTGCC AGAAACTGTC GGTGCCCTGC 
    ATGTCTTCCA AGCCCCAGAA GCCTTGGGAG AAAGATGCCT GGGAGATCCC TCGGGAATCC 
    CTCAAGCTGG AGAAGAAACT TGGAGCTGGG CAGTTTGGGG AAGTCTGGAT GGCCACCTAC 
    AACAAGCACA CCAAGGTGGC AGTGAAGACG ATGAAGCCAG GGAGCATGTC GGTGGAGGCC 
    TTCCTGGCAG AGGCCAACGT GATGAAAACT CTGCAGCATG ACAAGCTGGT CAAACTTCAT 
    GCGGTGGTCA CCAAGGAGCC CATCTACATC ATCACGGAGT TCATGGCCAA AGGAAGCTTG 
    CTGGACTTTC TGAAAAGTGA TGAGGGCAGC AAGCAGCCAT TGCCAAAACT CATTGACTTC 
    TCAGCCCAGA TTGCAGAAGG CATGGCCTTC ATCGAGCAGA GGAACTACAT CCACCGAGAC 
    CTCCGAGCTG CCAACATCTT GGTCTCTGCA TCCCTGGTGT GTAAGATTGC TGACTTTGGC 
    CTGGCCCGGG TCATTGAGGA CAACGAGTAC ACGGCTCGGG AAGGGGCCAA GTTCCCCATC 
    AAGTGGACAG CTCCTGAAGC CATCAACTTT GGCTCCTTCA CCATCAAGTC AGACGTCTGG 
    TCCTTTGGTA TCCTGCTGAT GGAGATCGTC ACCTACGGCC GGATCCCTTA CCCAGGGATG 
    TCAAACCCTG AAGTGATCCG AGCTCTGGAG CGTGGATACC GGATGCCTCG CCCAGAGAAC 
    TGCCCAGAGG AGCTCTACAA CATCATGATG CGCTGCTGGA AAAACCGTCC GGAGGAGCGG 
    CCGACCTTCG AATACATCCA GAGTGTGCTG GATGACTTCT ACACGGCCAC AGAGAGCCAG 
    TACCAACAGC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    HCK ( NM_001172130.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165601.1 Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu28541
    Clone ID Related Accession (Same CDS sequence) NM_001172130.1 , NM_001172130.2
    Accession Version NM_001172130.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1578bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Homo sapiens(human)
    Product tyrosine-protein kinase HCK isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA465978.1, BC113854.1, AK289896.1 and AW139272.1. On Jun 2, 2019 this sequence version replaced NM_001172130.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This variant encodes two isoforms due to the use of alternative translation initiation codons, as demonstrated in PMIDs 1875927 and 7791757. The longer isoform (c) is derived from an upstream non-AUG (CUG) start codon, while the shorter isoform (d) is derived from a downstream AUG start codon. The longer isoform (c) is represented in this RefSeq, but it is overall shorter, compared to isoform a. CCDS Note: This CCDS, which is supported by the mRNAs AK289896.1 and BC113854.1, represents a long human HCK isoform, as described in PMIDs 1875927 and 7791757. This isoform initiates translation from a non-AUG (CUG) start codon that is well-conserved and present in a strong Kozak signal context. Alternative translation initiation from a downstream AUG start codon produces an isoform that is 21 aa shorter at the N-terminus. The shorter isoform encoded by this variant is represented by CCDS 54456.1. These isoforms exhibit distinct subcellular distributions, as indicated in PMID:7791757. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.76670.1, SRR7410570.662727.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 7791757, 1875927 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    CTGGGGGGGC GCTCAAGCTG CGAGGATCCG GGCTGCCCGC GAGACGAGGA GCGGGCGCCC 
    AGGATGGGGT GCATGAAGTC CAAGTTCCTC CAGGTCGGAG GCAATACATT CTCAAAAACT 
    GAAACCAGCG CCAGCCCACA CTGTCCTGTG TACGTGCCGG ATCCCACATC CACCATCAAG 
    CCGGGGCCTA ATAGCCACAA CAGCAACACA CCAGGAATCA GGGAGGGCTC TGAGGACATC 
    ATCGTGGTTG CCCTGTATGA TTACGAGGCC ATTCACCACG AAGACCTCAG CTTCCAGAAG 
    GGGGACCAGA TGGTGGTCCT AGAGGAATCC GGGGAGTGGT GGAAGGCTCG ATCCCTGGCC 
    ACCCGGAAGG AGGGCTACAT CCCAAGCAAC TATGTCGCCC GCGTTGACTC TCTGGAGACA 
    GAGGAGTGGT TTTTCAAGGG CATCAGCCGG AAGGACGCAG AGCGCCAACT GCTGGCTCCC 
    GGCAACATGC TGGGCTCCTT CATGATCCGG GATAGCGAGA CCACTAAAGG AAGCTACTCT 
    TTGTCCGTGC GAGACTACGA CCCTCGGCAG GGAGATACCG TGAAACATTA CAAGATCCGG 
    ACCCTGGACA ACGGGGGCTT CTACATATCC CCCCGAAGCA CCTTCAGCAC TCTGCAGGAG 
    CTGGTGGACC ACTACAAGAA GGGGAACGAC GGGCTCTGCC AGAAACTGTC GGTGCCCTGC 
    ATGTCTTCCA AGCCCCAGAA GCCTTGGGAG AAAGATGCCT GGGAGATCCC TCGGGAATCC 
    CTCAAGCTGG AGAAGAAACT TGGAGCTGGG CAGTTTGGGG AAGTCTGGAT GGCCACCTAC 
    AACAAGCACA CCAAGGTGGC AGTGAAGACG ATGAAGCCAG GGAGCATGTC GGTGGAGGCC 
    TTCCTGGCAG AGGCCAACGT GATGAAAACT CTGCAGCATG ACAAGCTGGT CAAACTTCAT 
    GCGGTGGTCA CCAAGGAGCC CATCTACATC ATCACGGAGT TCATGGCCAA AGGAAGCTTG 
    CTGGACTTTC TGAAAAGTGA TGAGGGCAGC AAGCAGCCAT TGCCAAAACT CATTGACTTC 
    TCAGCCCAGA TTGCAGAAGG CATGGCCTTC ATCGAGCAGA GGAACTACAT CCACCGAGAC 
    CTCCGAGCTG CCAACATCTT GGTCTCTGCA TCCCTGGTGT GTAAGATTGC TGACTTTGGC 
    CTGGCCCGGG TCATTGAGGA CAACGAGTAC ACGGCTCGGG AAGGGGCCAA GTTCCCCATC 
    AAGTGGACAG CTCCTGAAGC CATCAACTTT GGCTCCTTCA CCATCAAGTC AGACGTCTGG 
    TCCTTTGGTA TCCTGCTGAT GGAGATCGTC ACCTACGGCC GGATCCCTTA CCCAGGGATG 
    TCAAACCCTG AAGTGATCCG AGCTCTGGAG CGTGGATACC GGATGCCTCG CCCAGAGAAC 
    TGCCCAGAGG AGCTCTACAA CATCATGATG CGCTGCTGGA AAAACCGTCC GGAGGAGCGG 
    CCGACCTTCG AATACATCCA GAGTGTGCTG GATGACTTCT ACACGGCCAC AGAGAGCCAG 
    TACCAACAGC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165601.1
    CDS184..1761
    Translation

    Target ORF information:

    RefSeq Version NM_001172130.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001172130.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    CTGGGGGGGC GCTCAAGCTG CGAGGATCCG GGCTGCCCGC GAGACGAGGA GCGGGCGCCC 
    AGGATGGGGT GCATGAAGTC CAAGTTCCTC CAGGTCGGAG GCAATACATT CTCAAAAACT 
    GAAACCAGCG CCAGCCCACA CTGTCCTGTG TACGTGCCGG ATCCCACATC CACCATCAAG 
    CCGGGGCCTA ATAGCCACAA CAGCAACACA CCAGGAATCA GGGAGGGCTC TGAGGACATC 
    ATCGTGGTTG CCCTGTATGA TTACGAGGCC ATTCACCACG AAGACCTCAG CTTCCAGAAG 
    GGGGACCAGA TGGTGGTCCT AGAGGAATCC GGGGAGTGGT GGAAGGCTCG ATCCCTGGCC 
    ACCCGGAAGG AGGGCTACAT CCCAAGCAAC TATGTCGCCC GCGTTGACTC TCTGGAGACA 
    GAGGAGTGGT TTTTCAAGGG CATCAGCCGG AAGGACGCAG AGCGCCAACT GCTGGCTCCC 
    GGCAACATGC TGGGCTCCTT CATGATCCGG GATAGCGAGA CCACTAAAGG AAGCTACTCT 
    TTGTCCGTGC GAGACTACGA CCCTCGGCAG GGAGATACCG TGAAACATTA CAAGATCCGG 
    ACCCTGGACA ACGGGGGCTT CTACATATCC CCCCGAAGCA CCTTCAGCAC TCTGCAGGAG 
    CTGGTGGACC ACTACAAGAA GGGGAACGAC GGGCTCTGCC AGAAACTGTC GGTGCCCTGC 
    ATGTCTTCCA AGCCCCAGAA GCCTTGGGAG AAAGATGCCT GGGAGATCCC TCGGGAATCC 
    CTCAAGCTGG AGAAGAAACT TGGAGCTGGG CAGTTTGGGG AAGTCTGGAT GGCCACCTAC 
    AACAAGCACA CCAAGGTGGC AGTGAAGACG ATGAAGCCAG GGAGCATGTC GGTGGAGGCC 
    TTCCTGGCAG AGGCCAACGT GATGAAAACT CTGCAGCATG ACAAGCTGGT CAAACTTCAT 
    GCGGTGGTCA CCAAGGAGCC CATCTACATC ATCACGGAGT TCATGGCCAA AGGAAGCTTG 
    CTGGACTTTC TGAAAAGTGA TGAGGGCAGC AAGCAGCCAT TGCCAAAACT CATTGACTTC 
    TCAGCCCAGA TTGCAGAAGG CATGGCCTTC ATCGAGCAGA GGAACTACAT CCACCGAGAC 
    CTCCGAGCTG CCAACATCTT GGTCTCTGCA TCCCTGGTGT GTAAGATTGC TGACTTTGGC 
    CTGGCCCGGG TCATTGAGGA CAACGAGTAC ACGGCTCGGG AAGGGGCCAA GTTCCCCATC 
    AAGTGGACAG CTCCTGAAGC CATCAACTTT GGCTCCTTCA CCATCAAGTC AGACGTCTGG 
    TCCTTTGGTA TCCTGCTGAT GGAGATCGTC ACCTACGGCC GGATCCCTTA CCCAGGGATG 
    TCAAACCCTG AAGTGATCCG AGCTCTGGAG CGTGGATACC GGATGCCTCG CCCAGAGAAC 
    TGCCCAGAGG AGCTCTACAA CATCATGATG CGCTGCTGGA AAAACCGTCC GGAGGAGCGG 
    CCGACCTTCG AATACATCCA GAGTGTGCTG GATGACTTCT ACACGGCCAC AGAGAGCCAG 
    TACCAACAGC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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