HBE1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HBE1
Entrez Gene ID	3046
Full Name	hemoglobin subunit epsilon 1
Synonyms	HBE
General protein information	Preferred Names hemoglobin subunit epsilon 1 Names hemoglobin subunit epsilon epsilon globin hemoglobin epsilon chain hemoglobin, epsilon 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.4
Summary	The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3' [provided by RefSeq, Jul 2008].
Disorder MIM:	142100

mRNA and Protein(s)

mRNA	Protein	Name
XM_011520036.1	XP_011518338.1	hemoglobin subunit epsilon isoform X1
NM_005330.4	NP_005321.1	hemoglobin subunit epsilon
NM_005330.3	NP_005321.1	hemoglobin subunit epsilon

Pathways from BioSystems

Homologies

Bos taurus (cattle)	HBE4	NP_001014910.1
Rattus norvegicus (Norway rat)	Hbg1	NP_742090.1
Danio rerio (zebrafish)	ba1l	NP_001013045.1
Danio rerio (zebrafish)	hbbe1.1	NP_571834.1
Danio rerio (zebrafish)	zgc:92880	XP_005164394.1
Gallus gallus (chicken)	HBG1	NP_001004390.1
Gallus gallus (chicken)	HBE	NP_001075173.1
Danio rerio (zebrafish)	ba1	NP_571095.1
Danio rerio (zebrafish)	hbbe3	NP_001015058.1
Danio rerio (zebrafish)	LOC573653	NP_001091054.1
Canis lupus familiaris (dog)	LOC485255	XP_542373.3
Gallus gallus (chicken)	HBE1	NP_001026660.1
Danio rerio (zebrafish)	hbbe2	NP_998011.1
Danio rerio (zebrafish)	MGC173646	NP_001096600.1
Pan troglodytes (chimpanzee)	HBE1	NP_001129304.1
	HBE1	NP_001040612.1
Bos taurus (cattle)	HBE2	NP_001014913.1
Gallus gallus (chicken)	HBG2	NP_990820.1
Homo sapiens (human)	HBE1	NP_005321.1
Mus musculus (house mouse)	Hbb-bh1	NP_032245.1

Gene Ontology
Bibliography

Related articles in PubMed

Heterochromatin Protein 1? Is a Novel Epigenetic Repressor of Human Embryonic ?-Globin Gene Expression.
Wang Y, Wang Y, Ma L, Nie M, Ju J, Liu M, Deng Y, Yao B, Gui T, Li X, Guo C, Ma C, Tan R, Zhao Q
The Journal of biological chemistry292(12)4811-4817(2017 03)

?-globin expression is regulated by SUV4-20h1.
Wang Y, Rank G, Li Z, Wang Y, Ju J, Nuber A, Wu Y, Liu M, Nie M, Huang F, Cerruti L, Ma C, Tan R, Schotta G, Jane SM, Zeng CK, Zhao Q
Haematologica101(5)e168-72(2016 05)

Epsilon globin gene expression in developing human fetal tissues.
McConaghy S, Manuel V, Nagji S, Ohls RK
Journal of neonatal-perinatal medicine9(1)91-7(2016)

Analysis of the Xmn1-(G) ? polymorphism in ?-thalassemia/hemoglobin E (HbE) and homozygous HbE patients with low and high levels of HbF.
Prasing W, Odawara T, Traisathit P, Yamashiro Y, Hattori Y, Pornprasert S
International journal of laboratory hematology37(2)e25-8(2015 Apr)

Transcriptional regulators Myb and BCL11A interplay with DNA methyltransferase 1 in developmental silencing of embryonic and fetal ?-like globin genes.
Roosjen M, McColl B, Kao B, Gearing LJ, Blewitt ME, Vadolas J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology28(4)1610-20(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data indicate that HP1-gamma is a novel epigenetic repressor of epsilon-globin gene expression.
Title: Heterochromatin Protein 1? Is a Novel Epigenetic Repressor of Human Embryonic ?-Globin Gene Expression.

Fetal varepsilon-globin gene expression is significantly greater than adult expression and is increased in maternal plasma compared to non-pregnant samples.
Title: Epsilon globin gene expression in developing human fetal tissues.

findings indicate the Xmn1-Ggamma polymorphism is likely to be one factor that influences the production of HbF in beta-thalassemia/HbE patients, but not in homozygous HbE patients
Title: Analysis of the Xmn1-(G) ? polymorphism in ?-thalassemia/hemoglobin E (HbE) and homozygous HbE patients with low and high levels of HbF.

Data indicate that Myb and BCL11A cooperate with DNMT1 to achieve developmental repression of embryonic and fetal beta-like human transgenic globin genes in the adult erythroid environment.
Title: Transcriptional regulators Myb and BCL11A interplay with DNA methyltransferase 1 in developmental silencing of embryonic and fetal ?-like globin genes.

Data indicate that in embryonic stem cells (hESCs)-derived erythroblasts where both epsilon and gamma globin were active, epsilon globin was immediately silenced upon transfer, whereas gamma globin continued to be expressed for months.
Title: Transcriptional environment and chromatin architecture interplay dictates globin expression patterns of heterospecific hybrids derived from undifferentiated human embryonic stem cells or from their erythroid progeny.

The following HBE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HBE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18235	NM_005330.4 Latest version!	Homo sapiens hemoglobin subunit epsilon 1 (HBE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu18235	XM_011520036.1 Latest version!	Homo sapiens hemoglobin, epsilon 1 (HBE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu18235	NM_005330.3	Homo sapiens hemoglobin subunit epsilon 1 (HBE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HBE1 ( NM_005330.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005321.1 Homo sapiens hemoglobin subunit epsilon 1 (HBE1), mRNA.

Price & Availability↑

CloneID	OHu18235
Clone ID Related Accession (Same CDS sequence)	XM_011520036.1 , NM_005330.4 , NM_005330.3
Accession Version	NM_005330.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	hemoglobin subunit epsilon
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104389.8. This sequence is a reference standard in the RefSeqGene project. On Nov 26, 2018 this sequence version replaced NM_005330.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.5364.1, BG496579.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03267750, SAMN03267752 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000396895.3/ ENSP00000380104.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGCATT TTACTGCTGA GGAGAAGGCT GCCGTCACTA GCCTGTGGAG CAAGATGAAT 
GTGGAAGAGG CTGGAGGTGA AGCCTTGGGC AGACTCCTCG TTGTTTACCC CTGGACCCAG 
AGATTTTTTG ACAGCTTTGG AAACCTGTCG TCTCCCTCTG CCATCCTGGG CAACCCCAAG 
GTCAAGGCCC ATGGCAAGAA GGTGCTGACT TCCTTTGGAG ATGCTATTAA AAACATGGAC 
AACCTCAAGC CCGCCTTTGC TAAGCTGAGT GAGCTGCACT GTGACAAGCT GCATGTGGAT 
CCTGAGAACT TCAAGCTCCT GGGTAACGTG ATGGTGATTA TTCTGGCTAC TCACTTTGGC 
AAGGAGTTCA CCCCTGAAGT GCAGGCTGCC TGGCAGAAGC TGGTGTCTGC TGTCGCCATT 
GCCCTGGCCC ATAAGTACCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005321.1
CDS	56..499
Translation	MVHFTAEEKAAVTSLWSKMNVEEAGGEALGRLLVVYPWTQRFFDSFGNLSSPSAILGNPKVKAHGKKVLTSFGDAIKNMDNLKPAFAKLSELHCDKLHVDPENFKLLGNVMVIILATHFGKEFTPEVQAAWQKLVSAVAIALAHKYH

Target ORF information:

RefSeq Version	NM_005330.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hemoglobin subunit epsilon 1 (HBE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005330.4

ORF Insert Sequence:

ATGGTGCATT TTACTGCTGA GGAGAAGGCT GCCGTCACTA GCCTGTGGAG CAAGATGAAT 
GTGGAAGAGG CTGGAGGTGA AGCCTTGGGC AGACTCCTCG TTGTTTACCC CTGGACCCAG 
AGATTTTTTG ACAGCTTTGG AAACCTGTCG TCTCCCTCTG CCATCCTGGG CAACCCCAAG 
GTCAAGGCCC ATGGCAAGAA GGTGCTGACT TCCTTTGGAG ATGCTATTAA AAACATGGAC 
AACCTCAAGC CCGCCTTTGC TAAGCTGAGT GAGCTGCACT GTGACAAGCT GCATGTGGAT 
CCTGAGAACT TCAAGCTCCT GGGTAACGTG ATGGTGATTA TTCTGGCTAC TCACTTTGGC 
AAGGAGTTCA CCCCTGAAGT GCAGGCTGCC TGGCAGAAGC TGGTGTCTGC TGTCGCCATT 
GCCCTGGCCC ATAAGTACCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HBE1 ( NM_005330.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005321.1 Homo sapiens hemoglobin subunit epsilon 1 (HBE1), mRNA.

Price & Availability↑

CloneID	OHu18235
Clone ID Related Accession (Same CDS sequence)	XM_011520036.1 , NM_005330.4 , NM_005330.3
Accession Version	NM_005330.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	hemoglobin subunit epsilon
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U01317.1. This sequence is a reference standard in the RefSeqGene project. On Feb 11, 2003 this sequence version replaced NM_005330.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CA336357.1, SRR1163658.67881.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03267750, SAMN03267752 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGTGCATT TTACTGCTGA GGAGAAGGCT GCCGTCACTA GCCTGTGGAG CAAGATGAAT 
GTGGAAGAGG CTGGAGGTGA AGCCTTGGGC AGACTCCTCG TTGTTTACCC CTGGACCCAG 
AGATTTTTTG ACAGCTTTGG AAACCTGTCG TCTCCCTCTG CCATCCTGGG CAACCCCAAG 
GTCAAGGCCC ATGGCAAGAA GGTGCTGACT TCCTTTGGAG ATGCTATTAA AAACATGGAC 
AACCTCAAGC CCGCCTTTGC TAAGCTGAGT GAGCTGCACT GTGACAAGCT GCATGTGGAT 
CCTGAGAACT TCAAGCTCCT GGGTAACGTG ATGGTGATTA TTCTGGCTAC TCACTTTGGC 
AAGGAGTTCA CCCCTGAAGT GCAGGCTGCC TGGCAGAAGC TGGTGTCTGC TGTCGCCATT 
GCCCTGGCCC ATAAGTACCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005321.1
CDS	254..697
Translation	MVHFTAEEKAAVTSLWSKMNVEEAGGEALGRLLVVYPWTQRFFDSFGNLSSPSAILGNPKVKAHGKKVLTSFGDAIKNMDNLKPAFAKLSELHCDKLHVDPENFKLLGNVMVIILATHFGKEFTPEVQAAWQKLVSAVAIALAHKYH

Target ORF information:

RefSeq Version	NM_005330.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hemoglobin subunit epsilon 1 (HBE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005330.3

ORF Insert Sequence:

ATGGTGCATT TTACTGCTGA GGAGAAGGCT GCCGTCACTA GCCTGTGGAG CAAGATGAAT 
GTGGAAGAGG CTGGAGGTGA AGCCTTGGGC AGACTCCTCG TTGTTTACCC CTGGACCCAG 
AGATTTTTTG ACAGCTTTGG AAACCTGTCG TCTCCCTCTG CCATCCTGGG CAACCCCAAG 
GTCAAGGCCC ATGGCAAGAA GGTGCTGACT TCCTTTGGAG ATGCTATTAA AAACATGGAC 
AACCTCAAGC CCGCCTTTGC TAAGCTGAGT GAGCTGCACT GTGACAAGCT GCATGTGGAT 
CCTGAGAACT TCAAGCTCCT GGGTAACGTG ATGGTGATTA TTCTGGCTAC TCACTTTGGC 
AAGGAGTTCA CCCCTGAAGT GCAGGCTGCC TGGCAGAAGC TGGTGTCTGC TGTCGCCATT 
GCCCTGGCCC ATAAGTACCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HBE1 ( XM_011520036.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518338.1 Homo sapiens hemoglobin, epsilon 1 (HBE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu18235
Clone ID Related Accession (Same CDS sequence)	XM_011520036.1 , NM_005330.4 , NM_005330.3
Accession Version	XM_011520036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	hemoglobin subunit epsilon isoform X1
Comment

ATGGTGCATT TTACTGCTGA GGAGAAGGCT GCCGTCACTA GCCTGTGGAG CAAGATGAAT 
GTGGAAGAGG CTGGAGGTGA AGCCTTGGGC AGACTCCTCG TTGTTTACCC CTGGACCCAG 
AGATTTTTTG ACAGCTTTGG AAACCTGTCG TCTCCCTCTG CCATCCTGGG CAACCCCAAG 
GTCAAGGCCC ATGGCAAGAA GGTGCTGACT TCCTTTGGAG ATGCTATTAA AAACATGGAC 
AACCTCAAGC CCGCCTTTGC TAAGCTGAGT GAGCTGCACT GTGACAAGCT GCATGTGGAT 
CCTGAGAACT TCAAGCTCCT GGGTAACGTG ATGGTGATTA TTCTGGCTAC TCACTTTGGC 
AAGGAGTTCA CCCCTGAAGT GCAGGCTGCC TGGCAGAAGC TGGTGTCTGC TGTCGCCATT 
GCCCTGGCCC ATAAGTACCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518338.1
Translation	MVHFTAEEKAAVTSLWSKMNVEEAGGEALGRLLVVYPWTQRFFDSFGNLSSPSAILGNPKVKAHGKKVLTSFGDAIKNMDNLKPAFAKLSELHCDKLHVDPENFKLLGNVMVIILATHFGKEFTPEVQAAWQKLVSAVAIALAHKYH

Target ORF information:

RefSeq Version	XM_011520036.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens hemoglobin, epsilon 1 (HBE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520036.1

ORF Insert Sequence:

ATGGTGCATT TTACTGCTGA GGAGAAGGCT GCCGTCACTA GCCTGTGGAG CAAGATGAAT 
GTGGAAGAGG CTGGAGGTGA AGCCTTGGGC AGACTCCTCG TTGTTTACCC CTGGACCCAG 
AGATTTTTTG ACAGCTTTGG AAACCTGTCG TCTCCCTCTG CCATCCTGGG CAACCCCAAG 
GTCAAGGCCC ATGGCAAGAA GGTGCTGACT TCCTTTGGAG ATGCTATTAA AAACATGGAC 
AACCTCAAGC CCGCCTTTGC TAAGCTGAGT GAGCTGCACT GTGACAAGCT GCATGTGGAT 
CCTGAGAACT TCAAGCTCCT GGGTAACGTG ATGGTGATTA TTCTGGCTAC TCACTTTGGC 
AAGGAGTTCA CCCCTGAAGT GCAGGCTGCC TGGCAGAAGC TGGTGTCTGC TGTCGCCATT 
GCCCTGGCCC ATAAGTACCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heterochromatin Protein 1? Is a Novel Epigenetic Repressor of Human Embryonic ?-Globin Gene Expression.
The Journal of biological chemistry292(12)4811-4817(2017 03)
Wang Y,Wang Y,Ma L,Nie M,Ju J,Liu M,Deng Y,Yao B,Gui T,Li X,Guo C,Ma C,Tan R,Zhao Q

?-globin expression is regulated by SUV4-20h1.
Haematologica101(5)e168-72(2016 05)
Wang Y,Rank G,Li Z,Wang Y,Ju J,Nuber A,Wu Y,Liu M,Nie M,Huang F,Cerruti L,Ma C,Tan R,Schotta G,Jane SM,Zeng CK,Zhao Q

Epsilon globin gene expression in developing human fetal tissues.
Journal of neonatal-perinatal medicine9(1)91-7(2016)
McConaghy S,Manuel V,Nagji S,Ohls RK

Analysis of the Xmn1-(G) ? polymorphism in ?-thalassemia/hemoglobin E (HbE) and homozygous HbE patients with low and high levels of HbF.
International journal of laboratory hematology37(2)e25-8(2015 Apr)
Prasing W,Odawara T,Traisathit P,Yamashiro Y,Hattori Y,Pornprasert S

Transcriptional regulators Myb and BCL11A interplay with DNA methyltransferase 1 in developmental silencing of embryonic and fetal ?-like globin genes.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology28(4)1610-20(2014 Apr)
Roosjen M,McColl B,Kao B,Gearing LJ,Blewitt ME,Vadolas J

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HBE1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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