HBB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HBB
Entrez Gene ID	3043
Full Name	hemoglobin subunit beta
Synonyms	CD113t-C,beta-globin
General protein information	Preferred Names hemoglobin subunit beta Names hemoglobin subunit beta beta globin chain hemoglobin, beta
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.4
Summary	The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008].
Disorder MIM:	141900
Disorder Html:	Sickle cell anemia, 603903 (3); Thalassemias, beta-, 613985 (3); Erythremias, beta- (3); Methemoglobinemias, beta- (3); Heinz body anemias, beta-, 140700 (3); Thalassemia-beta, dominant inclusion-body, 603902 (3); Hereditary persistence of fetal hemoglobin, 141749 (3); Delta-beta thalassemia, 141749 (3); {Malaria, resistance to}, 611162 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000518.4	NP_000509.1	hemoglobin subunit beta
NM_000518.5	NP_000509.1	hemoglobin subunit beta

Pathways from BioSystems

Homologies

Homo sapiens (human)	HBB	NP_000509.1
Pan troglodytes (chimpanzee)	HBB	XP_508242.1
Rattus norvegicus (Norway rat)	Hbb	NP_150237.1
Canis lupus familiaris (dog)	LOC476825	XP_534029.2
Mus musculus (house mouse)	Hbb-bs	NP_001188320.1
Mus musculus (house mouse)	Hbb-bt	NP_032246.2
	HBB	NP_001157900.1
Canis lupus familiaris (dog)	LOC609402	NP_001257812.1
Rattus norvegicus (Norway rat)	Hbb-b1	NP_942071.1
Canis lupus familiaris (dog)	LOC480784	NP_001257813.1
Rattus norvegicus (Norway rat)	LOC100134871	NP_001106694.1
Rattus norvegicus (Norway rat)	LOC689064	NP_001104739.1

Gene Ontology
Bibliography

Related articles in PubMed

Heterogeneity between Two ? Subunits of ?
Nagatomo S, Saito K, Yamamoto K, Ogura T, Kitagawa T, Nagai M
Biochemistry56(46)6125-6136(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that, in crystal form of Hb containing three protein molecules--Hbalpha(1)beta(2), allosteric effectors (phosphate, bezafibrate) induce conformational changes in direction from relaxed-state to tense-state; binding of each effector energetically stabilizes lowest affinity conformer more strongly than intermediate affinity conformer; minor Hb conformers have disproportionate effects on overall O(2) affinity.
Title: Direct observation of conformational population shifts in crystalline human hemoglobin.

Any alterations in the proximal CACCC and TATA boxes lead to a moderate decrease in synthesis of the beta-globin chain
Title: Phenotype of mutations in the promoter region of the ?-globin gene.

Five different beta-globin haplotypes were linked to sickle hemoglobin alleles in Mazandaran province.
Title: Identification of ?-globin haplotypes linked to sickle hemoglobin (Hb S) alleles in Mazandaran province, .

we concluded that this novel beta-globin gene mutation was associated with the mild phenotype of beta-thal.
Title: IVS-II-648/649 (-T) (HBB: c.316-202del) Triggers a Novel ?-Thalassemia Phenotype.

We screened for b-thalassemia-causing mutations by direct sequencing in different districts of West Bengal. Mutation detection was done using Sanger sequencing of the HBB gene. Results: A total of 14 different mutations were observed, including rare mutations IVS1-130(G>C), IVS1-129(A>C), -90(T>C), CD16(-C), -30(T>C), CD15(-T), and a novel mutation CD53(C>T).
Title: Cross-Sectional Study for the Detection of Mutations in the Beta-Globin Gene Among Patients with Hemoglobinopathies in the Bengali Population.

The following HBB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HBB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25936	NM_000518.5 Latest version!	Homo sapiens hemoglobin subunit beta (HBB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu25936	NM_000518.4	Homo sapiens hemoglobin subunit beta (HBB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HBB ( NM_000518.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000509.1 Homo sapiens hemoglobin subunit beta (HBB), mRNA.

Price & Availability↑

CloneID	OHu25936
Clone ID Related Accession (Same CDS sequence)	NM_000518.4 , NM_000518.5
Accession Version	NM_000518.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	hemoglobin subunit beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L48217.1. This sequence is a reference standard in the RefSeqGene project. On Feb 11, 2003 this sequence version replaced NM_000518.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: V00497.1, AU139015.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGTGCATC TGACTCCTGA GGAGAAGTCT GCCGTTACTG CCCTGTGGGG CAAGGTGAAC 
GTGGATGAAG TTGGTGGTGA GGCCCTGGGC AGGCTGCTGG TGGTCTACCC TTGGACCCAG 
AGGTTCTTTG AGTCCTTTGG GGATCTGTCC ACTCCTGATG CTGTTATGGG CAACCCTAAG 
GTGAAGGCTC ATGGCAAGAA AGTGCTCGGT GCCTTTAGTG ATGGCCTGGC TCACCTGGAC 
AACCTCAAGG GCACCTTTGC CACACTGAGT GAGCTGCACT GTGACAAGCT GCACGTGGAT 
CCTGAGAACT TCAGGCTCCT GGGCAACGTG CTGGTCTGTG TGCTGGCCCA TCACTTTGGC 
AAAGAATTCA CCCCACCAGT GCAGGCTGCC TATCAGAAAG TGGTGGCTGG TGTGGCTAAT 
GCCCTGGCCC ACAAGTATCA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000509.1
CDS	51..494
Translation	MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH

Target ORF information:

RefSeq Version	NM_000518.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hemoglobin subunit beta (HBB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000518.4

ORF Insert Sequence:

ATGGTGCATC TGACTCCTGA GGAGAAGTCT GCCGTTACTG CCCTGTGGGG CAAGGTGAAC 
GTGGATGAAG TTGGTGGTGA GGCCCTGGGC AGGCTGCTGG TGGTCTACCC TTGGACCCAG 
AGGTTCTTTG AGTCCTTTGG GGATCTGTCC ACTCCTGATG CTGTTATGGG CAACCCTAAG 
GTGAAGGCTC ATGGCAAGAA AGTGCTCGGT GCCTTTAGTG ATGGCCTGGC TCACCTGGAC 
AACCTCAAGG GCACCTTTGC CACACTGAGT GAGCTGCACT GTGACAAGCT GCACGTGGAT 
CCTGAGAACT TCAGGCTCCT GGGCAACGTG CTGGTCTGTG TGCTGGCCCA TCACTTTGGC 
AAAGAATTCA CCCCACCAGT GCAGGCTGCC TATCAGAAAG TGGTGGCTGG TGTGGCTAAT 
GCCCTGGCCC ACAAGTATCA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HBB ( NM_000518.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000509.1 Homo sapiens hemoglobin subunit beta (HBB), mRNA.

Price & Availability↑

CloneID	OHu25936
Clone ID Related Accession (Same CDS sequence)	NM_000518.4 , NM_000518.5
Accession Version	NM_000518.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	hemoglobin subunit beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK311825.1 and BU661647.1. This sequence is a reference standard in the RefSeqGene project. On Jun 13, 2018 this sequence version replaced NM_000518.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AU139015.1, SRR5189652.246572.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000335295.4/ ENSP00000333994.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGCATC TGACTCCTGA GGAGAAGTCT GCCGTTACTG CCCTGTGGGG CAAGGTGAAC 
GTGGATGAAG TTGGTGGTGA GGCCCTGGGC AGGCTGCTGG TGGTCTACCC TTGGACCCAG 
AGGTTCTTTG AGTCCTTTGG GGATCTGTCC ACTCCTGATG CTGTTATGGG CAACCCTAAG 
GTGAAGGCTC ATGGCAAGAA AGTGCTCGGT GCCTTTAGTG ATGGCCTGGC TCACCTGGAC 
AACCTCAAGG GCACCTTTGC CACACTGAGT GAGCTGCACT GTGACAAGCT GCACGTGGAT 
CCTGAGAACT TCAGGCTCCT GGGCAACGTG CTGGTCTGTG TGCTGGCCCA TCACTTTGGC 
AAAGAATTCA CCCCACCAGT GCAGGCTGCC TATCAGAAAG TGGTGGCTGG TGTGGCTAAT 
GCCCTGGCCC ACAAGTATCA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000509.1
CDS	51..494
Translation	MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH

Target ORF information:

RefSeq Version	NM_000518.5
Organism	Homo sapiens(human)
Definition	Homo sapiens hemoglobin subunit beta (HBB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000518.5

ORF Insert Sequence:

ATGGTGCATC TGACTCCTGA GGAGAAGTCT GCCGTTACTG CCCTGTGGGG CAAGGTGAAC 
GTGGATGAAG TTGGTGGTGA GGCCCTGGGC AGGCTGCTGG TGGTCTACCC TTGGACCCAG 
AGGTTCTTTG AGTCCTTTGG GGATCTGTCC ACTCCTGATG CTGTTATGGG CAACCCTAAG 
GTGAAGGCTC ATGGCAAGAA AGTGCTCGGT GCCTTTAGTG ATGGCCTGGC TCACCTGGAC 
AACCTCAAGG GCACCTTTGC CACACTGAGT GAGCTGCACT GTGACAAGCT GCACGTGGAT 
CCTGAGAACT TCAGGCTCCT GGGCAACGTG CTGGTCTGTG TGCTGGCCCA TCACTTTGGC 
AAAGAATTCA CCCCACCAGT GCAGGCTGCC TATCAGAAAG TGGTGGCTGG TGTGGCTAAT 
GCCCTGGCCC ACAAGTATCA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heterogeneity between Two ? Subunits of ?
Biochemistry56(46)6125-6136(2017 Nov)
Nagatomo S,Saito K,Yamamoto K,Ogura T,Kitagawa T,Nagai M

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HBB cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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