HBA2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HBA2
Entrez Gene ID	3040
Full Name	hemoglobin subunit alpha 2
Synonyms	HBA-T2,HBH
General protein information	Preferred Names hemoglobin subunit alpha 2 Names hemoglobin subunit alpha alpha globin alpha-2 globin hemoglobin alpha chain hemoglobin, alpha 2 mutant hemoglobin alpha 2 globin chain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq, Jul 2008].
Disorder MIM:	141850
Disorder Html:	Thalassemia, alpha-, 604131 (3); Heinz body anemia, 140700 (3); Erythrocytosis (3); Hypochromic microcytic anemia (3); Hemoglobin H disease, nondeletional, 613978 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000517.4	NP_000508.1	hemoglobin subunit alpha
NM_000517.6	NP_000508.1	hemoglobin subunit alpha

Pathways from BioSystems

Homologies

	HBA2	NP_001038189.1
Homo sapiens (human)	HBA2	NP_000508.1
Canis lupus familiaris (dog)	LOC100855540	NP_001257815.1
Mus musculus (house mouse)	Hba-a2	NP_001077424.1
Gallus gallus (chicken)	LOC100858011	XP_004949966.1
Xenopus tropicalis (tropical clawed frog)	hba1	NP_988860.1
	HBA2	XP_001094404.1
Canis lupus familiaris (dog)	LOC100855558	NP_001257814.1
Bos taurus (cattle)	HBA	NP_001070890.2
Bos taurus (cattle)	HBA1	XP_003585623.1
Homo sapiens (human)	HBA1	NP_000549.1
Gallus gallus (chicken)	HBAA	NP_001004376.1
Mus musculus (house mouse)	Hba-a1	NP_032244.2
Rattus norvegicus (Norway rat)	LOC287167	NP_001013875.1

Gene Ontology
Bibliography

Related articles in PubMed

Heterogeneity between Two ? Subunits of ?
Nagatomo S, Saito K, Yamamoto K, Ogura T, Kitagawa T, Nagai M
Biochemistry56(46)6125-6136(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In this paper, the experimental results of resonance Raman, terahertz time-domain, and 1H nuclear magnetic resonance spectroscopy for the Hb M are described in detail. The heterogeneity of alpha subunits, which has been observed previously, is reconfirmed, and its implications for the dynamical structure of the alpha2beta2 tetramer and the regulation of O2 affinity are discussed.
Title: Heterogeneity between Two ? Subunits of ?

Carriers of Hb Nanning are asymptomatic and could be missed by screening based on MCV and MCH cutoffs of <80.0 fL and <27.0 pg, respectively. Both carriers of the mutation in the study have MCV and MCH values that are 2 standard deviations (SDs) below normal, as would be predicted for an alpha+-thal.
Title: A Novel Mutation of the ?2-Globin Gene Causing ?

This study presents comparison of two neutron structures of hemoglobins, human deoxyhemoglobin (T state) and equine cyanomethemoglobin (R state) and offers a direct observation of histidine residues that are likely to contribute to the Bohr effect.
Title: Visualizing the Bohr effect in hemoglobin: neutron structure of equine cyanomethemoglobin in the R state and comparison with human deoxyhemoglobin in the T state.

Hb Adana mutation was preferentially present in the alpha2-globin genes in Malays compared to the other ethnicities in Malaysia.
Title: Analysis of ?1 and ?2 globin genes among patients with hemoglobin Adana in Malaysia.

Data indicate that binding of hemoblobin (Hb) to glycoprotein1balpha (GP1balpha) induced platelet activation plays a crucial role in thrombus formation on immobilized von Willebrand factor (VWF) or type I collagen under shear stresses.
Title: Inhibition of Hb Binding to GP1b? Abrogates Hb-Mediated Thrombus Formation on Immobilized VWF and Collagen under Physiological Shear Stress.

The following HBA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HBA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23534	NM_000517.6 Latest version!	Homo sapiens hemoglobin subunit alpha 2 (HBA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu23534	NM_000517.4	Homo sapiens hemoglobin subunit alpha 2 (HBA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HBA2 ( NM_000517.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000508.1 Homo sapiens hemoglobin subunit alpha 2 (HBA2), mRNA.

Price & Availability↑

CloneID	OHu23534
Clone ID Related Accession (Same CDS sequence)	NM_000517.4 , NM_000517.6
Accession Version	NM_000517.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	hemoglobin subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z84721.1 and BC008572.1. This sequence is a reference standard in the RefSeqGene project. On Apr 4, 2008 this sequence version replaced NM_000517.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI670460.1, BG434989.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGTGCTGT CTCCTGCCGA CAAGACCAAC GTCAAGGCCG CCTGGGGTAA GGTCGGCGCG 
CACGCTGGCG AGTATGGTGC GGAGGCCCTG GAGAGGATGT TCCTGTCCTT CCCCACCACC 
AAGACCTACT TCCCGCACTT CGACCTGAGC CACGGCTCTG CCCAGGTTAA GGGCCACGGC 
AAGAAGGTGG CCGACGCGCT GACCAACGCC GTGGCGCACG TGGACGACAT GCCCAACGCG 
CTGTCCGCCC TGAGCGACCT GCACGCGCAC AAGCTTCGGG TGGACCCGGT CAACTTCAAG 
CTCCTAAGCC ACTGCCTGCT GGTGACCCTG GCCGCCCACC TCCCCGCCGA GTTCACCCCT 
GCGGTGCACG CCTCCCTGGA CAAGTTCCTG GCTTCTGTGA GCACCGTGCT GACCTCCAAA 
TACCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000508.1
CDS	67..495
Translation	MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR

Target ORF information:

RefSeq Version	NM_000517.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hemoglobin subunit alpha 2 (HBA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000517.4

ORF Insert Sequence:

ATGGTGCTGT CTCCTGCCGA CAAGACCAAC GTCAAGGCCG CCTGGGGTAA GGTCGGCGCG 
CACGCTGGCG AGTATGGTGC GGAGGCCCTG GAGAGGATGT TCCTGTCCTT CCCCACCACC 
AAGACCTACT TCCCGCACTT CGACCTGAGC CACGGCTCTG CCCAGGTTAA GGGCCACGGC 
AAGAAGGTGG CCGACGCGCT GACCAACGCC GTGGCGCACG TGGACGACAT GCCCAACGCG 
CTGTCCGCCC TGAGCGACCT GCACGCGCAC AAGCTTCGGG TGGACCCGGT CAACTTCAAG 
CTCCTAAGCC ACTGCCTGCT GGTGACCCTG GCCGCCCACC TCCCCGCCGA GTTCACCCCT 
GCGGTGCACG CCTCCCTGGA CAAGTTCCTG GCTTCTGTGA GCACCGTGCT GACCTCCAAA 
TACCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HBA2 ( NM_000517.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_000508.1 Homo sapiens hemoglobin subunit alpha 2 (HBA2), mRNA.

Price & Availability↑

CloneID	OHu23534
Clone ID Related Accession (Same CDS sequence)	NM_000517.4 , NM_000517.6
Accession Version	NM_000517.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Homo sapiens(human)
Product	hemoglobin subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from V00493.1, Z84721.1 and AI016696.1. This sequence is a reference standard in the RefSeqGene project. On Aug 3, 2018 this sequence version replaced NM_000517.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AI816040.1, AI815806.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000251595.11/ ENSP00000251595.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGCTGT CTCCTGCCGA CAAGACCAAC GTCAAGGCCG CCTGGGGTAA GGTCGGCGCG 
CACGCTGGCG AGTATGGTGC GGAGGCCCTG GAGAGGATGT TCCTGTCCTT CCCCACCACC 
AAGACCTACT TCCCGCACTT CGACCTGAGC CACGGCTCTG CCCAGGTTAA GGGCCACGGC 
AAGAAGGTGG CCGACGCGCT GACCAACGCC GTGGCGCACG TGGACGACAT GCCCAACGCG 
CTGTCCGCCC TGAGCGACCT GCACGCGCAC AAGCTTCGGG TGGACCCGGT CAACTTCAAG 
CTCCTAAGCC ACTGCCTGCT GGTGACCCTG GCCGCCCACC TCCCCGCCGA GTTCACCCCT 
GCGGTGCACG CCTCCCTGGA CAAGTTCCTG GCTTCTGTGA GCACCGTGCT GACCTCCAAA 
TACCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000508.1
CDS	38..466
Translation	MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR

Target ORF information:

RefSeq Version	NM_000517.6
Organism	Homo sapiens(human)
Definition	Homo sapiens hemoglobin subunit alpha 2 (HBA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000517.6

ORF Insert Sequence:

ATGGTGCTGT CTCCTGCCGA CAAGACCAAC GTCAAGGCCG CCTGGGGTAA GGTCGGCGCG 
CACGCTGGCG AGTATGGTGC GGAGGCCCTG GAGAGGATGT TCCTGTCCTT CCCCACCACC 
AAGACCTACT TCCCGCACTT CGACCTGAGC CACGGCTCTG CCCAGGTTAA GGGCCACGGC 
AAGAAGGTGG CCGACGCGCT GACCAACGCC GTGGCGCACG TGGACGACAT GCCCAACGCG 
CTGTCCGCCC TGAGCGACCT GCACGCGCAC AAGCTTCGGG TGGACCCGGT CAACTTCAAG 
CTCCTAAGCC ACTGCCTGCT GGTGACCCTG GCCGCCCACC TCCCCGCCGA GTTCACCCCT 
GCGGTGCACG CCTCCCTGGA CAAGTTCCTG GCTTCTGTGA GCACCGTGCT GACCTCCAAA 
TACCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heterogeneity between Two ? Subunits of ?
Biochemistry56(46)6125-6136(2017 Nov)
Nagatomo S,Saito K,Yamamoto K,Ogura T,Kitagawa T,Nagai M

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HBA2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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