H1-4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	H1-4
Entrez Gene ID	3008
Full Name	histone cluster 1 H1 family member e
Synonyms	H1.4,H1E,H1F4,H1s-4,RMNS,dJ221C16.5
General protein information	Preferred Names histone cluster 1 H1 family member e Names histone H1.4 H1 histone family, member 4 histone 1, H1e histone H1b histone H1s-4 histone cluster 1, H1e
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p22.2
Summary	Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015].
Disorder MIM:	142220
Disorder Html:	Rahman syndrome, 617537 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_005321.2	NP_005312.1	histone H1.4
NM_005321.3	NP_005312.1	histone H1.4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC471881	XP_527259.1
Homo sapiens (human)	HIST1H1E	NP_005312.1
	LOC696872	XP_001086509.2
Bos taurus (cattle)	HIST1H1E	XP_002697564.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that histones H1.2 and H1.4 were observed in MDA-MB-231 metastatic breast cancer cells. The phosphorylation at S173 of histone H1.2 and S172, S187, T18, T146, and T154 of H1.4 significantly increases during M phase suggesting that these events are cell cycle-dependent.
Title: Quantitative Mass Spectrometry Reveals that Intact Histone H1 Phosphorylations are Variant Specific and Exhibit Single Molecule Hierarchical Dependence.

This study identified and confirmed HIST1H1E protein changes within the postsynaptic density in schizophrenia.
Title: Proteomic and genomic evidence implicates the postsynaptic density in schizophrenia.

Histone H1.2-T165 post translational modifications are dispensable for chromatin binding and cell proliferation while the H1.4-K26 modifications are essential for proper cell cycle progression.
Title: Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications.

Mutations in linker histone genes HIST1H1 B, C, D, and E; OCT2 (POU2F2); IRF8; and ARID1A underlying the pathogenesis of follicular lymphoma.
Title: Mutations in linker histone genes HIST1H1 B, C, D, and E; OCT2 (POU2F2); IRF8; and ARID1A underlying the pathogenesis of follicular lymphoma.

H1.4K34 acetylation is mediated by GCN5 and is preferentially enriched at promoters of active genes, where it stimulates transcription by increasing H1 mobility and recruiting a general transcription factor.
Title: A dual role of linker histone H1.4 Lys 34 acetylation in transcriptional activation.

The following H1-4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H1-4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23854	NM_005321.3 Latest version!	Homo sapiens H1.4 linker histone, cluster member (H1-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu23854	NM_005321.2	Homo sapiens histone cluster 1 H1 family member e (HIST1H1E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H1-4 ( NM_005321.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005312.1 Homo sapiens histone cluster 1 H1 family member e (HIST1H1E), mRNA.

Price & Availability↑

CloneID	OHu23854
Clone ID Related Accession (Same CDS sequence)	NM_005321.2 , NM_005321.3
Accession Version	NM_005321.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	histone H1.4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M60748.1. On May 13, 2002 this sequence version replaced NM_005321.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCGAGA CTGCGCCTGC CGCGCCCGCT GCTCCGGCCC CTGCCGAGAA GACTCCCGTG 
AAGAAGAAGG CCCGCAAGTC TGCAGGTGCG GCCAAGCGCA AAGCGTCTGG GCCCCCGGTG 
TCCGAGCTCA TTACTAAAGC TGTTGCCGCC TCCAAGGAGC GCAGCGGCGT ATCTTTGGCC 
GCTCTCAAGA AAGCGCTGGC AGCCGCTGGC TATGACGTGG AGAAGAACAA CAGCCGCATC 
AAGCTGGGTC TCAAGAGCCT GGTGAGCAAG GGCACCCTGG TGCAGACCAA GGGCACCGGC 
GCGTCGGGTT CCTTCAAACT CAACAAGAAG GCGGCCTCTG GGGAAGCCAA GCCTAAGGCT 
AAAAAGGCAG GCGCGGCCAA GGCCAAGAAG CCAGCAGGAG CGGCGAAGAA GCCCAAGAAG 
GCGACGGGGG CGGCCACCCC CAAGAAGAGC GCCAAGAAGA CCCCAAAGAA GGCGAAGAAG 
CCGGCTGCAG CTGCTGGAGC CAAAAAAGCG AAAAGCCCGA AAAAGGCGAA AGCAGCCAAG 
CCAAAAAAGG CGCCCAAGAG CCCAGCGAAG GCCAAAGCAG TTAAACCCAA GGCGGCTAAA 
CCAAAGACCG CCAAGCCCAA GGCAGCCAAG CCAAAGAAGG CGGCAGCCAA GAAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005312.1
CDS	61..720
Translation	MSETAPAAPAAPAPAEKTPVKKKARKSAGAAKRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKAKKAGAAKAKKPAGAAKKPKKATGAATPKKSAKKTPKKAKKPAAAAGAKKAKSPKKAKAAKPKKAPKSPAKAKAVKPKAAKPKTAKPKAAKPKKAAAKKK

Target ORF information:

RefSeq Version	NM_005321.2
Organism	Homo sapiens(human)
Definition	Homo sapiens histone cluster 1 H1 family member e (HIST1H1E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005321.2

ORF Insert Sequence:

ATGTCCGAGA CTGCGCCTGC CGCGCCCGCT GCTCCGGCCC CTGCCGAGAA GACTCCCGTG 
AAGAAGAAGG CCCGCAAGTC TGCAGGTGCG GCCAAGCGCA AAGCGTCTGG GCCCCCGGTG 
TCCGAGCTCA TTACTAAAGC TGTTGCCGCC TCCAAGGAGC GCAGCGGCGT ATCTTTGGCC 
GCTCTCAAGA AAGCGCTGGC AGCCGCTGGC TATGACGTGG AGAAGAACAA CAGCCGCATC 
AAGCTGGGTC TCAAGAGCCT GGTGAGCAAG GGCACCCTGG TGCAGACCAA GGGCACCGGC 
GCGTCGGGTT CCTTCAAACT CAACAAGAAG GCGGCCTCTG GGGAAGCCAA GCCTAAGGCT 
AAAAAGGCAG GCGCGGCCAA GGCCAAGAAG CCAGCAGGAG CGGCGAAGAA GCCCAAGAAG 
GCGACGGGGG CGGCCACCCC CAAGAAGAGC GCCAAGAAGA CCCCAAAGAA GGCGAAGAAG 
CCGGCTGCAG CTGCTGGAGC CAAAAAAGCG AAAAGCCCGA AAAAGGCGAA AGCAGCCAAG 
CCAAAAAAGG CGCCCAAGAG CCCAGCGAAG GCCAAAGCAG TTAAACCCAA GGCGGCTAAA 
CCAAAGACCG CCAAGCCCAA GGCAGCCAAG CCAAAGAAGG CGGCAGCCAA GAAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H1-4 ( NM_005321.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005312.1 Homo sapiens H1.4 linker histone, cluster member (H1-4), mRNA.

Price & Availability↑

CloneID	OHu23854
Clone ID Related Accession (Same CDS sequence)	NM_005321.2 , NM_005321.3
Accession Version	NM_005321.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	histone H1.4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL353759.8. On Dec 3, 2019 this sequence version replaced NM_005321.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: CR988914.1, BC099632.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCGAGA CTGCGCCTGC CGCGCCCGCT GCTCCGGCCC CTGCCGAGAA GACTCCCGTG 
AAGAAGAAGG CCCGCAAGTC TGCAGGTGCG GCCAAGCGCA AAGCGTCTGG GCCCCCGGTG 
TCCGAGCTCA TTACTAAAGC TGTTGCCGCC TCCAAGGAGC GCAGCGGCGT ATCTTTGGCC 
GCTCTCAAGA AAGCGCTGGC AGCCGCTGGC TATGACGTGG AGAAGAACAA CAGCCGCATC 
AAGCTGGGTC TCAAGAGCCT GGTGAGCAAG GGCACCCTGG TGCAGACCAA GGGCACCGGC 
GCGTCGGGTT CCTTCAAACT CAACAAGAAG GCGGCCTCTG GGGAAGCCAA GCCTAAGGCT 
AAAAAGGCAG GCGCGGCCAA GGCCAAGAAG CCAGCAGGAG CGGCGAAGAA GCCCAAGAAG 
GCGACGGGGG CGGCCACCCC CAAGAAGAGC GCCAAGAAGA CCCCAAAGAA GGCGAAGAAG 
CCGGCTGCAG CTGCTGGAGC CAAAAAAGCG AAAAGCCCGA AAAAGGCGAA AGCAGCCAAG 
CCAAAAAAGG CGCCCAAGAG CCCAGCGAAG GCCAAAGCAG TTAAACCCAA GGCGGCTAAA 
CCAAAGACCG CCAAGCCCAA GGCAGCCAAG CCAAAGAAGG CGGCAGCCAA GAAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005312.1
CDS	63..722
Translation	MSETAPAAPAAPAPAEKTPVKKKARKSAGAAKRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKAKKAGAAKAKKPAGAAKKPKKATGAATPKKSAKKTPKKAKKPAAAAGAKKAKSPKKAKAAKPKKAPKSPAKAKAVKPKAAKPKTAKPKAAKPKKAAAKKK

Target ORF information:

RefSeq Version	NM_005321.3
Organism	Homo sapiens(human)
Definition	Homo sapiens H1.4 linker histone, cluster member (H1-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005321.3

ORF Insert Sequence:

ATGTCCGAGA CTGCGCCTGC CGCGCCCGCT GCTCCGGCCC CTGCCGAGAA GACTCCCGTG 
AAGAAGAAGG CCCGCAAGTC TGCAGGTGCG GCCAAGCGCA AAGCGTCTGG GCCCCCGGTG 
TCCGAGCTCA TTACTAAAGC TGTTGCCGCC TCCAAGGAGC GCAGCGGCGT ATCTTTGGCC 
GCTCTCAAGA AAGCGCTGGC AGCCGCTGGC TATGACGTGG AGAAGAACAA CAGCCGCATC 
AAGCTGGGTC TCAAGAGCCT GGTGAGCAAG GGCACCCTGG TGCAGACCAA GGGCACCGGC 
GCGTCGGGTT CCTTCAAACT CAACAAGAAG GCGGCCTCTG GGGAAGCCAA GCCTAAGGCT 
AAAAAGGCAG GCGCGGCCAA GGCCAAGAAG CCAGCAGGAG CGGCGAAGAA GCCCAAGAAG 
GCGACGGGGG CGGCCACCCC CAAGAAGAGC GCCAAGAAGA CCCCAAAGAA GGCGAAGAAG 
CCGGCTGCAG CTGCTGGAGC CAAAAAAGCG AAAAGCCCGA AAAAGGCGAA AGCAGCCAAG 
CCAAAAAAGG CGCCCAAGAG CCCAGCGAAG GCCAAAGCAG TTAAACCCAA GGCGGCTAAA 
CCAAAGACCG CCAAGCCCAA GGCAGCCAAG CCAAAGAAGG CGGCAGCCAA GAAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

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H1-4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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