RAX cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAX
Entrez Gene ID	30062
Full Name	retina and anterior neural fold homeobox
Synonyms	MCOP3,RX
General protein information	Preferred Names retina and anterior neural fold homeobox Names retinal homeobox protein Rx retina and anterior neural fold homeobox protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18q21.32
Summary	This gene encodes a homeobox-containing transcription factor that functions in eye development. The gene is expressed early in the eye primordia, and is required for retinal cell fate determination and also regulates stem cell proliferation. Mutations in this gene have been reported in patients with defects in ocular development, including microphthalmia, anophthalmia, and coloboma.[provided by RefSeq, Oct 2009].
Disorder MIM:	601881
Disorder Html:	Microphthalmia, isolated 3, 611038 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_013435.3	NP_038463.2	retinal homeobox protein Rx
NM_013435.2	NP_038463.2	retinal homeobox protein Rx

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rax	NP_446130.1
	RAX	XP_001087324.1
Xenopus tropicalis (tropical clawed frog)	rax	XP_002936715.1
Mus musculus (house mouse)	Rax	NP_038861.2
Pan troglodytes (chimpanzee)	RAX	XP_001142510.1
Danio rerio (zebrafish)	rx3	NP_571302.1
Homo sapiens (human)	RAX	NP_038463.2
Canis lupus familiaris (dog)	RAX	XP_852642.2
Bos taurus (cattle)	RAX	XP_003587831.1
Gallus gallus (chicken)	RAX	NP_001230653.1

Gene Ontology
Bibliography

Related articles in PubMed

Unraveling the genetic cause of a consanguineous family with unilateral coloboma and retinoschisis: expanding the phenotypic variability of RAX mutations.
Huang XF, Huang ZQ, Lin D, Dai ML, Wang QF, Chen ZJ, Jin ZB, Wang Y
Scientific reports7(1)9064(2017 Aug)

RAX and anophthalmia in humans: evidence of brain anomalies.
Abouzeid H, Youssef MA, Bayoumi N, ElShakankiri N, Marzouk I, Hauser P, Schorderet DF
Molecular vision181449-56(2012)

Derivation of human differential photoreceptor-like cells from the iris by defined combinations of CRX, RX and NEUROD.
Seko Y, Azuma N, Kaneda M, Nakatani K, Miyagawa Y, Noshiro Y, Kurokawa R, Okano H, Umezawa A
PloS one7(4)e35611(2012)

Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.
Oshikawa M, Tsutsui C, Ikegami T, Fuchida Y, Matsubara M, Toyama S, Usami R, Ohtoko K, Kato S
Investigative ophthalmology & visual science52(9)6662-70(2011 Aug)

Mutational screening of CHX10, GDF6, OTX2, RAX and SOX2 genes in 50 unrelated microphthalmia-anophthalmia-coloboma (MAC) spectrum cases.
Gonzalez-Rodriguez J, Pelcastre EL, Tovilla-Canales JL, Garcia-Ortiz JE, Amato-Almanza M, Villanueva-Mendoza C, Espinosa-Mattar Z, Zenteno JC
The British journal of ophthalmology94(8)1100-4(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The three consanguineous Egyptian anophthalmia patients carry a novel homozygous c.543+3A>G mutation (IVS2+3A>G) in RAX.
Title: RAX and anophthalmia in humans: evidence of brain anomalies.

Photosensitive photoreceptor cells can be generated by combinations of transcription factors. The combination of CRX and RX generate immature photoreceptors: and additional NEUROD promotes maturation.
Title: Derivation of human differential photoreceptor-like cells from the iris by defined combinations of CRX, RX and NEUROD.

Observational study of gene-disease association. (HuGE Navigator)
Title: Mutational screening of CHX10, GDF6, OTX2, RAX and SOX2 genes in 50 unrelated microphthalmia-anophthalmia-coloboma (MAC) spectrum cases.

Observational study of gene-disease association. (HuGE Navigator)
Title: Mutational screening of 10 genes in Chinese patients with microphthalmia and/or coloboma.

Mutations in the newly identified RAX regulatory sequence are not a frequent cause of micro/anophthalmia.
Title: Mutations in the newly identified RAX regulatory sequence are not a frequent cause of micro/anophthalmia.

The following RAX gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAX cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19517	NM_013435.3 Latest version!	Homo sapiens retina and anterior neural fold homeobox (RAX), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu19517	NM_013435.2	Homo sapiens retina and anterior neural fold homeobox (RAX), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAX ( NM_013435.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_038463.2 Homo sapiens retina and anterior neural fold homeobox (RAX), mRNA.

Price & Availability↑

CloneID	OHu19517
Clone ID Related Accession (Same CDS sequence)	NM_013435.3 , NM_013435.2
Accession Version	NM_013435.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	retinal homeobox protein Rx
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC067859.7 and AF115392.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_013435.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF115392.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2147596, SAMEA2156670 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000334889.4/ ENSP00000334813.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCACCTGC CGGGCTGCGC GCCAGCCATG GCCGACGGGA GCTTCTCGCT TGCCGGCCAC 
CTGCTCCGCA GCCCGGGCGG GAGCACCTCG CGACTTCACA GCATCGAGGC CATCCTGGGG 
TTTACCAAGG ACGACGGGAT CCTCGGCACC TTCCCGGCGG AGCGGGGCGC CCGGGGCGCG 
AAGGAGCGGG ATAGGAGGCT GGGCGCGCGG CCCGCCTGCC CCAAGGCGCC CGAGGAAGGC 
TCCGAGCCCT CCCCGCCGCC AGCCCCGGCG CCCGCCCCCG AGTACGAAGC CCCTCGACCC 
TACTGCCCCA AGGAGCCCGG GGAGGCACGG CCGAGCCCAG GGCTGCCCGT CGGGCCAGCC 
ACCGGCGAAG CGAAACTGTC AGAGGAGGAA CAGCCCAAGA AAAAGCATCG GCGGAACCGC 
ACGACTTTCA CCACGTACCA GCTGCATGAG CTGGAGCGCG CGTTCGAGAA GTCCCACTAC 
CCGGACGTGT ACAGCCGCGA GGAGCTGGCC GGCAAGGTCA ACCTACCAGA GGTCCGGGTC 
CAGGTGTGGT TCCAGAACCG ACGGGCTAAG TGGCGGCGGC AGGAGAAGCT GGAAGTGTCC 
TCCATGAAGC TGCAGGACTC GCCCCTCCTC TCCTTCAGCC GCTCCCCGCC CTCCGCGACG 
CTGTCGCCCC TCGGGGCGGG CCCGGGCAGC GGTGGCGGGC CGGCTGGGGG CGCGCTGCCG 
CTGGAGTCCT GGCTCGGGCC GCCGCTGCCG GGCGGGGGCG CCACGGCGCT GCAGAGCCTG 
CCGGGCTTCG GGCCGCCGGC GCAGAGCCTG CCTGCCAGCT ACACGCCACC GCCGCCGCCT 
CCGCCCTTCC TGAACTCCCC GCCGTTGGGC CCCGGCCTGC AACCTCTCGC GCCGCCGCCG 
CCCTCCTACC CGTGCGGGCC CGGCTTCGGG GACAAGTTCC CGCTGGACGA GGCGGACCCG 
CGCAACAGCA GCATCGCGGC GCTGCGTCTG AAAGCCAAGG AGCACATCCA GGCCATCGGG 
AAGCCGTGGC AGGCCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038463.2
CDS	249..1289
Translation	MHLPGCAPAMADGSFSLAGHLLRSPGGSTSRLHSIEAILGFTKDDGILGTFPAERGARGAKERDRRLGARPACPKAPEEGSEPSPPPAPAPAPEYEAPRPYCPKEPGEARPSPGLPVGPATGEAKLSEEEQPKKKHRRNRTTFTTYQLHELERAFEKSHYPDVYSREELAGKVNLPEVRVQVWFQNRRAKWRRQEKLEVSSMKLQDSPLLSFSRSPPSATLSPLGAGPGSGGGPAGGALPLESWLGPPLPGGGATALQSLPGFGPPAQSLPASYTPPPPPPPFLNSPPLGPGLQPLAPPPPSYPCGPGFGDKFPLDEADPRNSSIAALRLKAKEHIQAIGKPWQAL

Target ORF information:

RefSeq Version	NM_013435.3
Organism	Homo sapiens(human)
Definition	Homo sapiens retina and anterior neural fold homeobox (RAX), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013435.3

ORF Insert Sequence:

ATGCACCTGC CGGGCTGCGC GCCAGCCATG GCCGACGGGA GCTTCTCGCT TGCCGGCCAC 
CTGCTCCGCA GCCCGGGCGG GAGCACCTCG CGACTTCACA GCATCGAGGC CATCCTGGGG 
TTTACCAAGG ACGACGGGAT CCTCGGCACC TTCCCGGCGG AGCGGGGCGC CCGGGGCGCG 
AAGGAGCGGG ATAGGAGGCT GGGCGCGCGG CCCGCCTGCC CCAAGGCGCC CGAGGAAGGC 
TCCGAGCCCT CCCCGCCGCC AGCCCCGGCG CCCGCCCCCG AGTACGAAGC CCCTCGACCC 
TACTGCCCCA AGGAGCCCGG GGAGGCACGG CCGAGCCCAG GGCTGCCCGT CGGGCCAGCC 
ACCGGCGAAG CGAAACTGTC AGAGGAGGAA CAGCCCAAGA AAAAGCATCG GCGGAACCGC 
ACGACTTTCA CCACGTACCA GCTGCATGAG CTGGAGCGCG CGTTCGAGAA GTCCCACTAC 
CCGGACGTGT ACAGCCGCGA GGAGCTGGCC GGCAAGGTCA ACCTACCAGA GGTCCGGGTC 
CAGGTGTGGT TCCAGAACCG ACGGGCTAAG TGGCGGCGGC AGGAGAAGCT GGAAGTGTCC 
TCCATGAAGC TGCAGGACTC GCCCCTCCTC TCCTTCAGCC GCTCCCCGCC CTCCGCGACG 
CTGTCGCCCC TCGGGGCGGG CCCGGGCAGC GGTGGCGGGC CGGCTGGGGG CGCGCTGCCG 
CTGGAGTCCT GGCTCGGGCC GCCGCTGCCG GGCGGGGGCG CCACGGCGCT GCAGAGCCTG 
CCGGGCTTCG GGCCGCCGGC GCAGAGCCTG CCTGCCAGCT ACACGCCACC GCCGCCGCCT 
CCGCCCTTCC TGAACTCCCC GCCGTTGGGC CCCGGCCTGC AACCTCTCGC GCCGCCGCCG 
CCCTCCTACC CGTGCGGGCC CGGCTTCGGG GACAAGTTCC CGCTGGACGA GGCGGACCCG 
CGCAACAGCA GCATCGCGGC GCTGCGTCTG AAAGCCAAGG AGCACATCCA GGCCATCGGG 
AAGCCGTGGC AGGCCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAX ( NM_013435.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_038463.2 Homo sapiens retina and anterior neural fold homeobox (RAX), mRNA.

Price & Availability↑

CloneID	OHu19517
Clone ID Related Accession (Same CDS sequence)	NM_013435.3 , NM_013435.2
Accession Version	NM_013435.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	retinal homeobox protein Rx
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF115392.1 and AC067859.7. This sequence is a reference standard in the RefSeqGene project. On Feb 23, 2007 this sequence version replaced NM_013435.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF115392.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2147596, SAMEA2156670 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACCTGC CGGGCTGCGC GCCAGCCATG GCCGACGGGA GCTTCTCGCT TGCCGGCCAC 
CTGCTCCGCA GCCCGGGCGG GAGCACCTCG CGACTTCACA GCATCGAGGC CATCCTGGGG 
TTTACCAAGG ACGACGGGAT CCTCGGCACC TTCCCGGCGG AGCGGGGCGC CCGGGGCGCG 
AAGGAGCGGG ATAGGAGGCT GGGCGCGCGG CCCGCCTGCC CCAAGGCGCC CGAGGAAGGC 
TCCGAGCCCT CCCCGCCGCC AGCCCCGGCG CCCGCCCCCG AGTACGAAGC CCCTCGACCC 
TACTGCCCCA AGGAGCCCGG GGAGGCACGG CCGAGCCCAG GGCTGCCCGT CGGGCCAGCC 
ACCGGCGAAG CGAAACTGTC AGAGGAGGAA CAGCCCAAGA AAAAGCATCG GCGGAACCGC 
ACGACTTTCA CCACGTACCA GCTGCATGAG CTGGAGCGCG CGTTCGAGAA GTCCCACTAC 
CCGGACGTGT ACAGCCGCGA GGAGCTGGCC GGCAAGGTCA ACCTACCAGA GGTCCGGGTC 
CAGGTGTGGT TCCAGAACCG ACGGGCTAAG TGGCGGCGGC AGGAGAAGCT GGAAGTGTCC 
TCCATGAAGC TGCAGGACTC GCCCCTCCTC TCCTTCAGCC GCTCCCCGCC CTCCGCGACG 
CTGTCGCCCC TCGGGGCGGG CCCGGGCAGC GGTGGCGGGC CGGCTGGGGG CGCGCTGCCG 
CTGGAGTCCT GGCTCGGGCC GCCGCTGCCG GGCGGGGGCG CCACGGCGCT GCAGAGCCTG 
CCGGGCTTCG GGCCGCCGGC GCAGAGCCTG CCTGCCAGCT ACACGCCACC GCCGCCGCCT 
CCGCCCTTCC TGAACTCCCC GCCGTTGGGC CCCGGCCTGC AACCTCTCGC GCCGCCGCCG 
CCCTCCTACC CGTGCGGGCC CGGCTTCGGG GACAAGTTCC CGCTGGACGA GGCGGACCCG 
CGCAACAGCA GCATCGCGGC GCTGCGTCTG AAAGCCAAGG AGCACATCCA GGCCATCGGG 
AAGCCGTGGC AGGCCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038463.2
CDS	188..1228
Translation	MHLPGCAPAMADGSFSLAGHLLRSPGGSTSRLHSIEAILGFTKDDGILGTFPAERGARGAKERDRRLGARPACPKAPEEGSEPSPPPAPAPAPEYEAPRPYCPKEPGEARPSPGLPVGPATGEAKLSEEEQPKKKHRRNRTTFTTYQLHELERAFEKSHYPDVYSREELAGKVNLPEVRVQVWFQNRRAKWRRQEKLEVSSMKLQDSPLLSFSRSPPSATLSPLGAGPGSGGGPAGGALPLESWLGPPLPGGGATALQSLPGFGPPAQSLPASYTPPPPPPPFLNSPPLGPGLQPLAPPPPSYPCGPGFGDKFPLDEADPRNSSIAALRLKAKEHIQAIGKPWQAL

Target ORF information:

RefSeq Version	NM_013435.2
Organism	Homo sapiens(human)
Definition	Homo sapiens retina and anterior neural fold homeobox (RAX), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013435.2

ORF Insert Sequence:

ATGCACCTGC CGGGCTGCGC GCCAGCCATG GCCGACGGGA GCTTCTCGCT TGCCGGCCAC 
CTGCTCCGCA GCCCGGGCGG GAGCACCTCG CGACTTCACA GCATCGAGGC CATCCTGGGG 
TTTACCAAGG ACGACGGGAT CCTCGGCACC TTCCCGGCGG AGCGGGGCGC CCGGGGCGCG 
AAGGAGCGGG ATAGGAGGCT GGGCGCGCGG CCCGCCTGCC CCAAGGCGCC CGAGGAAGGC 
TCCGAGCCCT CCCCGCCGCC AGCCCCGGCG CCCGCCCCCG AGTACGAAGC CCCTCGACCC 
TACTGCCCCA AGGAGCCCGG GGAGGCACGG CCGAGCCCAG GGCTGCCCGT CGGGCCAGCC 
ACCGGCGAAG CGAAACTGTC AGAGGAGGAA CAGCCCAAGA AAAAGCATCG GCGGAACCGC 
ACGACTTTCA CCACGTACCA GCTGCATGAG CTGGAGCGCG CGTTCGAGAA GTCCCACTAC 
CCGGACGTGT ACAGCCGCGA GGAGCTGGCC GGCAAGGTCA ACCTACCAGA GGTCCGGGTC 
CAGGTGTGGT TCCAGAACCG ACGGGCTAAG TGGCGGCGGC AGGAGAAGCT GGAAGTGTCC 
TCCATGAAGC TGCAGGACTC GCCCCTCCTC TCCTTCAGCC GCTCCCCGCC CTCCGCGACG 
CTGTCGCCCC TCGGGGCGGG CCCGGGCAGC GGTGGCGGGC CGGCTGGGGG CGCGCTGCCG 
CTGGAGTCCT GGCTCGGGCC GCCGCTGCCG GGCGGGGGCG CCACGGCGCT GCAGAGCCTG 
CCGGGCTTCG GGCCGCCGGC GCAGAGCCTG CCTGCCAGCT ACACGCCACC GCCGCCGCCT 
CCGCCCTTCC TGAACTCCCC GCCGTTGGGC CCCGGCCTGC AACCTCTCGC GCCGCCGCCG 
CCCTCCTACC CGTGCGGGCC CGGCTTCGGG GACAAGTTCC CGCTGGACGA GGCGGACCCG 
CGCAACAGCA GCATCGCGGC GCTGCGTCTG AAAGCCAAGG AGCACATCCA GGCCATCGGG 
AAGCCGTGGC AGGCCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Unraveling the genetic cause of a consanguineous family with unilateral coloboma and retinoschisis: expanding the phenotypic variability of RAX mutations.
Scientific reports7(1)9064(2017 Aug)
Huang XF,Huang ZQ,Lin D,Dai ML,Wang QF,Chen ZJ,Jin ZB,Wang Y

RAX and anophthalmia in humans: evidence of brain anomalies.
Molecular vision181449-56(2012)
Abouzeid H,Youssef MA,Bayoumi N,ElShakankiri N,Marzouk I,Hauser P,Schorderet DF

Derivation of human differential photoreceptor-like cells from the iris by defined combinations of CRX, RX and NEUROD.
PloS one7(4)e35611(2012)
Seko Y,Azuma N,Kaneda M,Nakatani K,Miyagawa Y,Noshiro Y,Kurokawa R,Okano H,Umezawa A

Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.
Investigative ophthalmology & visual science52(9)6662-70(2011 Aug)
Oshikawa M,Tsutsui C,Ikegami T,Fuchida Y,Matsubara M,Toyama S,Usami R,Ohtoko K,Kato S

Mutational screening of CHX10, GDF6, OTX2, RAX and SOX2 genes in 50 unrelated microphthalmia-anophthalmia-coloboma (MAC) spectrum cases.
The British journal of ophthalmology94(8)1100-4(2010 Aug)
Gonzalez-Rodriguez J,Pelcastre EL,Tovilla-Canales JL,Garcia-Ortiz JE,Amato-Almanza M,Villanueva-Mendoza C,Espinosa-Mattar Z,Zenteno JC

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RAX cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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