GZMA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GZMA
Entrez Gene ID	3001
Full Name	granzyme A
Synonyms	CTLA3,HFSP
General protein information	Preferred Names granzyme A Names granzyme A CTL tryptase Cytotoxic T-lymphocyte-associated serine esterase-3 Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease) HF Hanukah factor serine protease) cytotoxic T-lymphocyte proteinase 1 fragmentin-1 granzyme 1 granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) h factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q11.2
Summary	Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008].
Disorder MIM:	140050

mRNA and Protein(s)

mRNA	Protein	Name
NM_006144.3	NP_006135.1	granzyme A precursor
NM_006144.4	NP_006135.2	granzyme A precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	GZMA	NP_006135.1
Canis lupus familiaris (dog)	GZMA	XP_544335.3
Bos taurus (cattle)	GZMA	NP_001092565.1
Rattus norvegicus (Norway rat)	Gzma	NP_703198.1
Xenopus tropicalis (tropical clawed frog)	LOC100497188	XP_002941159.1
Gallus gallus (chicken)	GZMA	NP_989788.1
Pan troglodytes (chimpanzee)	GZMA	XP_001148261.1
Mus musculus (house mouse)	Gzma	NP_034500.1
	GZMA	XP_001097639.1

Gene Ontology
Bibliography

Related articles in PubMed

Granzyme A in Human Platelets Regulates the Synthesis of Proinflammatory Cytokines by Monocytes in Aging.
Campbell RA, Franks Z, Bhatnagar A, Rowley JW, Manne BK, Supiano MA, Schwertz H, Weyrich AS, Rondina MT
Journal of immunology (Baltimore, Md. : 1950)200(1)295-304(2018 Jan)

Granzyme A potentiates chemokine production in IL-17-stimulated keratinocytes.
Cheuk S, Martini E, Bergh K, Chang D, Rethi B, St?hle M, Eidsmo L
Experimental dermatology26(9)824-827(2017 Sep)

Granzyme A impairs host defense during Streptococcus pneumoniae pneumonia.
van den Boogaard FE, van Gisbergen KP, Vernooy JH, Medema JP, Roelofs JJ, van Zoelen MA, Endeman H, Biesma DH, Boon L, Van't Veer C, de Vos AF, van der Poll T
American journal of physiology. Lung cellular and molecular physiology311(2)L507-16(2016 Aug)

Killer lymphocytes use granulysin, perforin and granzymes to kill intracellular parasites.
Dotiwala F, Mulik S, Polidoro RB, Ansara JA, Burleigh BA, Walch M, Gazzinelli RT, Lieberman J
Nature medicine22(2)210-6(2016 Feb)

Association Between Response to Etrolizumab and Expression of Integrin ?E and Granzyme A in Colon Biopsies of Patients With Ulcerative Colitis.
Tew GW, Hackney JA, Gibbons D, Lamb CA, Luca D, Egen JG, Diehl L, Eastham Anderson J, Vermeire S, Mansfield JC, Feagan BG, Panes J, Baumgart DC, Schreiber S, Dotan I, Sandborn WJ, Kirby JA, Irving PM, De Hertogh G, Van Assche GA, Rutgeerts P, O'Byrne S, Hayday A, Keir ME
Gastroenterology150(2)477-87.e9(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

granzyme A in human platelets increases with aging
Title: Granzyme A in Human Platelets Regulates the Synthesis of Proinflammatory Cytokines by Monocytes in Aging.

GzmA plays an unfavorable role in host defense during pneumococcal pneumonia by a mechanism that does not depend on natural killer cells.
Title: Granzyme A impairs host defense during Streptococcus pneumoniae pneumonia.

Delivered into parasite infected cells by granulysin and perforin, granzymes generate superoxide and inactivate oxidative defense enzymes to kill the parasite.
Title: Killer lymphocytes use granulysin, perforin and granzymes to kill intracellular parasites.

Levels of GZMA and ITGAE mRNAs in colon tissues can identify patients with UC who are most likely to benefit from etrolizumab; expression levels decrease with etrolizumab administration in biomarker(high) patients.
Title: Association Between Response to Etrolizumab and Expression of Integrin ?E and Granzyme A in Colon Biopsies of Patients With Ulcerative Colitis.

Results reveal enhanced intra- and extracellular expression of gzmA and B in patients with pulmonary TB, suggesting that gzms are part of the host response to tuberculosis.
Title: Increased intra- and extracellular granzyme expression in patients with tuberculosis.

The following GZMA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GZMA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu26824	NM_006144.3 Latest version!	Homo sapiens granzyme A (GZMA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu107343	NM_006144.4 Latest version!	Homo sapiens granzyme A (GZMA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GZMA ( NM_006144.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006135.1 Homo sapiens granzyme A (GZMA), mRNA.

Price & Availability↑

CloneID	OHu26824
Clone ID Related Accession (Same CDS sequence)	NM_006144.3
Accession Version	NM_006144.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	granzyme A precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP330335.1 and BC015739.1. This sequence is a reference standard in the RefSeqGene project. On Jul 11, 2008 this sequence version replaced NM_006144.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015739.1, BQ053520.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGAACT CCTATAGATT TCTGGCATCC TCTCTCTCAG TTGTCGTTTC TCTCCTGCTA 
ATTCCTGAAG ATGTCTGTGA AAAAATTATT GGAGGAAATG AAGTAACTCC TCATTCAAGA 
CCCTACATGG TCCTACTTAG TCTTGACAGA AAAACCATCT GTGCTGGGGC TTTGATTGCA 
AAAGACTGGG TGTTGACTGC AGCTCACTGT AACTTGAACA AAAGGTCCCA GGTCATTCTT 
GGGGCTCACT CAATAACCAG GGAAGAGCCA ACAAAACAGA TAATGCTTGT TAAGAAAGAG 
TTTCCCTATC CATGCTATGA CCCAGCCACA CGCGAAGGTG ACCTTAAACT TTTACAGCTG 
ACGGAAAAAG CAAAAATTAA CAAATATGTG ACTATCCTTC ATCTACCTAA AAAGGGGGAT 
GATGTGAAAC CAGGAACCAT GTGCCAAGTT GCAGGGTGGG GCAGGACTCA CAATAGTGCA 
TCTTGGTCCG ATACTCTGAG AGAAGTCAAT ATCACCATCA TAGACAGAAA AGTCTGCAAT 
GATCGAAATC ACTATAATTT TAACCCTGTG ATTGGAATGA ATATGGTTTG TGCTGGAAGC 
CTCCGAGGTG GAAGAGACTC GTGCAATGGA GATTCTGGAA GCCCTTTGTT GTGCGAGGGT 
GTTTTCCGAG GGGTCACTTC CTTTGGCCTT GAAAATAAAT GCGGAGACCC TCGTGGGCCT 
GGTGTCTATA TTCTTCTCTC AAAGAAACAC CTCAACTGGA TAATTATGAC TATCAAGGGA 
GCAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006135.1
CDS	38..826
Translation	MRNSYRFLASSLSVVVSLLLIPEDVCEKIIGGNEVTPHSRPYMVLLSLDRKTICAGALIAKDWVLTAAHCNLNKRSQVILGAHSITREEPTKQIMLVKKEFPYPCYDPATREGDLKLLQLTEKAKINKYVTILHLPKKGDDVKPGTMCQVAGWGRTHNSASWSDTLREVNITIIDRKVCNDRNHYNFNPVIGMNMVCAGSLRGGRDSCNGDSGSPLLCEGVFRGVTSFGLENKCGDPRGPGVYILLSKKHLNWIIMTIKGAV

Target ORF information:

RefSeq Version	NM_006144.3
Organism	Homo sapiens(human)
Definition	Homo sapiens granzyme A (GZMA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006144.3

ORF Insert Sequence:

ATGAGGAACT CCTATAGATT TCTGGCATCC TCTCTCTCAG TTGTCGTTTC TCTCCTGCTA 
ATTCCTGAAG ATGTCTGTGA AAAAATTATT GGAGGAAATG AAGTAACTCC TCATTCAAGA 
CCCTACATGG TCCTACTTAG TCTTGACAGA AAAACCATCT GTGCTGGGGC TTTGATTGCA 
AAAGACTGGG TGTTGACTGC AGCTCACTGT AACTTGAACA AAAGGTCCCA GGTCATTCTT 
GGGGCTCACT CAATAACCAG GGAAGAGCCA ACAAAACAGA TAATGCTTGT TAAGAAAGAG 
TTTCCCTATC CATGCTATGA CCCAGCCACA CGCGAAGGTG ACCTTAAACT TTTACAGCTG 
ACGGAAAAAG CAAAAATTAA CAAATATGTG ACTATCCTTC ATCTACCTAA AAAGGGGGAT 
GATGTGAAAC CAGGAACCAT GTGCCAAGTT GCAGGGTGGG GCAGGACTCA CAATAGTGCA 
TCTTGGTCCG ATACTCTGAG AGAAGTCAAT ATCACCATCA TAGACAGAAA AGTCTGCAAT 
GATCGAAATC ACTATAATTT TAACCCTGTG ATTGGAATGA ATATGGTTTG TGCTGGAAGC 
CTCCGAGGTG GAAGAGACTC GTGCAATGGA GATTCTGGAA GCCCTTTGTT GTGCGAGGGT 
GTTTTCCGAG GGGTCACTTC CTTTGGCCTT GAAAATAAAT GCGGAGACCC TCGTGGGCCT 
GGTGTCTATA TTCTTCTCTC AAAGAAACAC CTCAACTGGA TAATTATGAC TATCAAGGGA 
GCAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GZMA ( NM_006144.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006135.2 Homo sapiens granzyme A (GZMA), mRNA.

Price & Availability↑

CloneID	OHu107343
Clone ID Related Accession (Same CDS sequence)	NM_006144.4
Accession Version	NM_006144.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	granzyme A precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC091977.3. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_006144.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015739.1, SRR5189667.281315.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000274306.7/ ENSP00000274306.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGAACT CCTATAGATT TCTGGCATCC TCTCTCTCAG TTGTCGTTTC TCTCCTGCTA 
ATTCCTGAAG ATGTCTGTGA AAAAATTATT GGAGGAAATG AAGTAACTCC TCATTCAAGA 
CCCTACATGG TCCTACTTAG TCTTGACAGA AAAACCATCT GTGCTGGGGC TTTGATTGCA 
AAAGACTGGG TGTTGACTGC AGCTCACTGT AACTTGAACA AAAGGTCCCA GGTCATTCTT 
GGGGCTCACT CAATAACCAG GGAAGAGCCA ACAAAACAGA TAATGCTTGT TAAGAAAGAG 
TTTCCCTATC CATGCTATGA CCCAGCCACA CGCGAAGGTG ACCTTAAACT TTTACAGCTG 
ATGGAAAAAG CAAAAATTAA CAAATATGTG ACTATCCTTC ATCTACCTAA AAAGGGGGAC 
GATGTGAAAC CAGGAACCAT GTGCCAAGTT GCAGGGTGGG GCAGGACTCA CAATAGTGCA 
TCTTGGTCCG ATACTCTGAG AGAAGTCAAT ATCACCATCA TAGACAGAAA AGTCTGCAAT 
GATCGAAATC ACTATAATTT TAACCCTGTG ATTGGAATGA ATATGGTTTG TGCTGGAAGC 
CTCCGAGGTG GAAGAGACTC GTGCAATGGA GATTCTGGAA GCCCTTTGTT GTGCGAGGGT 
GTTTTCCGAG GGGTCACTTC CTTTGGCCTT GAAAATAAAT GCGGAGACCC TCGTGGGCCT 
GGTGTCTATA TTCTTCTCTC AAAGAAACAC CTCAACTGGA TAATTATGAC TATCAAGGGA 
GCAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006135.2
CDS	38..826
Translation	MRNSYRFLASSLSVVVSLLLIPEDVCEKIIGGNEVTPHSRPYMVLLSLDRKTICAGALIAKDWVLTAAHCNLNKRSQVILGAHSITREEPTKQIMLVKKEFPYPCYDPATREGDLKLLQLMEKAKINKYVTILHLPKKGDDVKPGTMCQVAGWGRTHNSASWSDTLREVNITIIDRKVCNDRNHYNFNPVIGMNMVCAGSLRGGRDSCNGDSGSPLLCEGVFRGVTSFGLENKCGDPRGPGVYILLSKKHLNWIIMTIKGAV

Target ORF information:

RefSeq Version	NM_006144.4
Organism	Homo sapiens(human)
Definition	Homo sapiens granzyme A (GZMA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006144.4

ORF Insert Sequence:

ATGAGGAACT CCTATAGATT TCTGGCATCC TCTCTCTCAG TTGTCGTTTC TCTCCTGCTA 
ATTCCTGAAG ATGTCTGTGA AAAAATTATT GGAGGAAATG AAGTAACTCC TCATTCAAGA 
CCCTACATGG TCCTACTTAG TCTTGACAGA AAAACCATCT GTGCTGGGGC TTTGATTGCA 
AAAGACTGGG TGTTGACTGC AGCTCACTGT AACTTGAACA AAAGGTCCCA GGTCATTCTT 
GGGGCTCACT CAATAACCAG GGAAGAGCCA ACAAAACAGA TAATGCTTGT TAAGAAAGAG 
TTTCCCTATC CATGCTATGA CCCAGCCACA CGCGAAGGTG ACCTTAAACT TTTACAGCTG 
ATGGAAAAAG CAAAAATTAA CAAATATGTG ACTATCCTTC ATCTACCTAA AAAGGGGGAC 
GATGTGAAAC CAGGAACCAT GTGCCAAGTT GCAGGGTGGG GCAGGACTCA CAATAGTGCA 
TCTTGGTCCG ATACTCTGAG AGAAGTCAAT ATCACCATCA TAGACAGAAA AGTCTGCAAT 
GATCGAAATC ACTATAATTT TAACCCTGTG ATTGGAATGA ATATGGTTTG TGCTGGAAGC 
CTCCGAGGTG GAAGAGACTC GTGCAATGGA GATTCTGGAA GCCCTTTGTT GTGCGAGGGT 
GTTTTCCGAG GGGTCACTTC CTTTGGCCTT GAAAATAAAT GCGGAGACCC TCGTGGGCCT 
GGTGTCTATA TTCTTCTCTC AAAGAAACAC CTCAACTGGA TAATTATGAC TATCAAGGGA 
GCAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Granzyme A in Human Platelets Regulates the Synthesis of Proinflammatory Cytokines by Monocytes in Aging.
Journal of immunology (Baltimore, Md. : 1950)200(1)295-304(2018 Jan)
Campbell RA,Franks Z,Bhatnagar A,Rowley JW,Manne BK,Supiano MA,Schwertz H,Weyrich AS,Rondina MT

Granzyme A potentiates chemokine production in IL-17-stimulated keratinocytes.
Experimental dermatology26(9)824-827(2017 Sep)
Cheuk S,Martini E,Bergh K,Chang D,Rethi B,St?hle M,Eidsmo L

Granzyme A impairs host defense during Streptococcus pneumoniae pneumonia.
American journal of physiology. Lung cellular and molecular physiology311(2)L507-16(2016 Aug)
van den Boogaard FE,van Gisbergen KP,Vernooy JH,Medema JP,Roelofs JJ,van Zoelen MA,Endeman H,Biesma DH,Boon L,Van't Veer C,de Vos AF,van der Poll T

Killer lymphocytes use granulysin, perforin and granzymes to kill intracellular parasites.
Nature medicine22(2)210-6(2016 Feb)
Dotiwala F,Mulik S,Polidoro RB,Ansara JA,Burleigh BA,Walch M,Gazzinelli RT,Lieberman J

Association Between Response to Etrolizumab and Expression of Integrin ?E and Granzyme A in Colon Biopsies of Patients With Ulcerative Colitis.
Gastroenterology150(2)477-87.e9(2016 Feb)
Tew GW,Hackney JA,Gibbons D,Lamb CA,Luca D,Egen JG,Diehl L,Eastham Anderson J,Vermeire S,Mansfield JC,Feagan BG,Panes J,Baumgart DC,Schreiber S,Dotan I,Sandborn WJ,Kirby JA,Irving PM,De Hertogh G,Van Assche GA,Rutgeerts P,O'Byrne S,Hayday A,Keir ME

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GZMA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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