LMCD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LMCD1
Entrez Gene ID	29995
Full Name	LIM and cysteine rich domains 1
General protein information	Preferred Names LIM and cysteine rich domains 1 Names LIM and cysteine-rich domains protein 1 dyxin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p25.3
Summary	This gene encodes a member of the LIM-domain family of zinc finger proteins. The encoded protein contains an N-terminal cysteine-rich domain and two C-terminal LIM domains. The presence of LIM domains suggests involvement in protein-protein interactions. The protein may act as a co-regulator of transcription along with other transcription factors. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013].
Disorder MIM:	604859

mRNA and Protein(s)

mRNA	Protein	Name
XM_011533649.1	XP_011531951.1	LIM and cysteine-rich domains protein 1 isoform X1
NM_001278235.2	NP_001265164.1	LIM and cysteine-rich domains protein 1 isoform 4
NM_001278233.2	NP_001265162.1	LIM and cysteine-rich domains protein 1 isoform 2
NM_001278234.1	NP_001265163.1	LIM and cysteine-rich domains protein 1 isoform 3
NM_014583.3	NP_055398.1	LIM and cysteine-rich domains protein 1 isoform 1
NM_001278233.1	NP_001265162.1	LIM and cysteine-rich domains protein 1 isoform 2
NM_001278235.1	NP_001265164.1	LIM and cysteine-rich domains protein 1 isoform 4
NM_014583.4	NP_055398.1	LIM and cysteine-rich domains protein 1 isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LMCD1	XP_516253.3
Mus musculus (house mouse)	Lmcd1	NP_659048.1
Gallus gallus (chicken)	LMCD1	XP_414443.2
Rattus norvegicus (Norway rat)	Lmcd1	NP_001008562.1
Danio rerio (zebrafish)	lmcd1	NP_957364.2
Homo sapiens (human)	LMCD1	NP_055398.1
Canis lupus familiaris (dog)	LMCD1	XP_533751.3
Bos taurus (cattle)	LMCD1	NP_001069690.1

Gene Ontology
Bibliography

Related articles in PubMed

LIM and cysteine-rich domains 1 is required for thrombin-induced smooth muscle cell proliferation and promotes atherogenesis.
Janjanam J, Zhang B, Mani AM, Singh NK, Traylor JG, Orr AW, Rao GN
The Journal of biological chemistry(2018 Jan)

A common variant association study reveals novel susceptibility loci for low HDL-cholesterol levels in ethnic Arabs.
Wakil SM, Ram R, Muiya NP, Andres E, Mazhar N, Hagos S, Alshahid M, Meyer BF, Morahan G, Dzimiri N
Clinical genetics90(6)518-525(2016 Dec)

Somatic LMCD1 mutations promoted cell migration and tumor metastasis in hepatocellular carcinoma.
Chang CY, Lin SC, Su WH, Ho CM, Jou YS
Oncogene31(21)2640-52(2012 May)

Image-based genome-wide siRNA screen identifies selective autophagy factors.
Orvedahl A, Sumpter R Jr, Xiao G, Ng A, Zou Z, Tang Y, Narimatsu M, Gilpin C, Sun Q, Roth M, Forst CV, Wrana JL, Zhang YE, Luby-Phelps K, Xavier RJ, Xie Y, Levine B
Nature480(7375)113-7(2011 Dec)

Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for GATA6.
Donati C, Marseglia G, Magi A, Serrat? S, Cencetti F, Bernacchioni C, Nannetti G, Benelli M, Brunelli S, Torricelli F, Cossu G, Bruni P
PloS one6(5)e20389(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The rs1800775 [1.31 (1.22-1.42); p = 3.41E-12] in the CETP gene and rs359027 [1.26 (1.16-1.36); p = 2.55E-08] in the LMCD1 gene were significantly associated with LHDLC levels
Title: A common variant association study reveals novel susceptibility loci for low HDL-cholesterol levels in ethnic Arabs.

Together, our results suggest that LMCD1 mutations are potential oncogenic events in HCC metastasis to promote cell migration through the Rac1-signaling pathway.
Title: Somatic LMCD1 mutations promoted cell migration and tumor metastasis in hepatocellular carcinoma.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following LMCD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMCD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00589	NM_014583.4 Latest version!	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu04377	NM_001278233.2 Latest version!	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu04378	NM_001278234.1 Latest version!	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu04379	NM_001278235.2 Latest version!	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu67460	XM_011533649.1 Latest version!	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu04377	NM_001278233.1	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu04379	NM_001278235.1	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu00589	NM_014583.3	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LMCD1 ( NM_014583.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055398.1 Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00589
Clone ID Related Accession (Same CDS sequence)	NM_014583.3 , NM_014583.4
Accession Version	NM_014583.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	LIM and cysteine-rich domains protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN388104.1, AF169284.1 and BU619403.1. On May 21, 2013 this sequence version replaced NM_014583.2. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660805.150890.1, SRR1803614.63208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAAGG TGGCTAAGGA CCTCAACCCA GGAGTTAAAA AGATGTCCCT GGGCCAGCTG 
CAGTCAGCAA GAGGTGTGGC ATGTTTGGGA TGCAAGGGGA CGTGTTCGGG CTTCGAGCCA 
CATTCATGGA GGAAAATATG CAAGTCTTGC AAATGCAGCC AAGAGGACCA CTGCCTAACA 
TCTGACCTAG AAGACGATCG GAAAATTGGC CGCTTGCTGA TGGACTCCAA GTATTCCACC 
CTCACTGCTC GGGTGAAAGG CGGGGACGGC ATCCGGATTT ACAAGAGGAA CCGGATGATC 
ATGACCAACC CTATTGCTAC TGGGAAAGAT CCCACTTTTG ACACCATCAC CTACGAGTGG 
GCTCCCCCTG GAGTCACCCA GAAACTGGGA CTGCAGTACA TGGAGCTCAT CCCCAAGGAG 
AAGCAGCCAG TGACAGGCAC AGAGGGTGCC TTTTACCGCC GCCGCCAGCT CATGCACCAG 
CTCCCCATCT ATGACCAGGA TCCCTCGCGC TGCCGTGGAC TTTTGGAGAA TGAGTTGAAA 
CTGATGGAAG AATTTGTCAA GCAATATAAG AGCGAGGCCC TCGGCGTGGG AGAAGTGGCC 
CTCCCGGGGC AGGGTGGCTT GCCCAAGGAG GAGGGGAAGC AGCAGGAAAA GCCAGAGGGG 
GCAGAGACCA CTGCTGCTAC CACCAACGGC AGTCTCAGTG ACCCGTCCAA AGAAGTGGAA 
TACGTCTGCG AGCTCTGCAA GGGAGCGGCC CCTCCTGACA GCCCCGTGGT CTACTCGGAC 
AGGGCAGGCT ACAACAAGCA GTGGCACCCC ACCTGCTTTG TGTGTGCCAA GTGCTCCGAG 
CCGCTGGTGG ACCTCATCTA CTTCTGGAAG GATGGTGCAC CCTGGTGCGG CCGCCATTAC 
TGCGAGAGTC TGCGGCCCCG GTGCTCCGGC TGCGATGAGA TAATATTCGC TGAGGACTAC 
CAGCGTGTGG AAGATCTGGC CTGGCACCGA AAGCACTTTG TCTGTGAGGG TTGTGAGCAG 
CTGCTGAGCG GCCGGGCGTA CATCGTCACC AAGGGTCAGC TTCTGTGCCC AACTTGCAGC 
AAGTCCAAAC GCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055398.1
CDS	133..1230
Translation	MAKVAKDLNPGVKKMSLGQLQSARGVACLGCKGTCSGFEPHSWRKICKSCKCSQEDHCLTSDLEDDRKIGRLLMDSKYSTLTARVKGGDGIRIYKRNRMIMTNPIATGKDPTFDTITYEWAPPGVTQKLGLQYMELIPKEKQPVTGTEGAFYRRRQLMHQLPIYDQDPSRCRGLLENELKLMEEFVKQYKSEALGVGEVALPGQGGLPKEEGKQQEKPEGAETTAATTNGSLSDPSKEVEYVCELCKGAAPPDSPVVYSDRAGYNKQWHPTCFVCAKCSEPLVDLIYFWKDGAPWCGRHYCESLRPRCSGCDEIIFAEDYQRVEDLAWHRKHFVCEGCEQLLSGRAYIVTKGQLLCPTCSKSKRS

Target ORF information:

RefSeq Version	NM_014583.3
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014583.3

ORF Insert Sequence:

ATGGCAAAGG TGGCTAAGGA CCTCAACCCA GGAGTTAAAA AGATGTCCCT GGGCCAGCTG 
CAGTCAGCAA GAGGTGTGGC ATGTTTGGGA TGCAAGGGGA CGTGTTCGGG CTTCGAGCCA 
CATTCATGGA GGAAAATATG CAAGTCTTGC AAATGCAGCC AAGAGGACCA CTGCCTAACA 
TCTGACCTAG AAGACGATCG GAAAATTGGC CGCTTGCTGA TGGACTCCAA GTATTCCACC 
CTCACTGCTC GGGTGAAAGG CGGGGACGGC ATCCGGATTT ACAAGAGGAA CCGGATGATC 
ATGACCAACC CTATTGCTAC TGGGAAAGAT CCCACTTTTG ACACCATCAC CTACGAGTGG 
GCTCCCCCTG GAGTCACCCA GAAACTGGGA CTGCAGTACA TGGAGCTCAT CCCCAAGGAG 
AAGCAGCCAG TGACAGGCAC AGAGGGTGCC TTTTACCGCC GCCGCCAGCT CATGCACCAG 
CTCCCCATCT ATGACCAGGA TCCCTCGCGC TGCCGTGGAC TTTTGGAGAA TGAGTTGAAA 
CTGATGGAAG AATTTGTCAA GCAATATAAG AGCGAGGCCC TCGGCGTGGG AGAAGTGGCC 
CTCCCGGGGC AGGGTGGCTT GCCCAAGGAG GAGGGGAAGC AGCAGGAAAA GCCAGAGGGG 
GCAGAGACCA CTGCTGCTAC CACCAACGGC AGTCTCAGTG ACCCGTCCAA AGAAGTGGAA 
TACGTCTGCG AGCTCTGCAA GGGAGCGGCC CCTCCTGACA GCCCCGTGGT CTACTCGGAC 
AGGGCAGGCT ACAACAAGCA GTGGCACCCC ACCTGCTTTG TGTGTGCCAA GTGCTCCGAG 
CCGCTGGTGG ACCTCATCTA CTTCTGGAAG GATGGTGCAC CCTGGTGCGG CCGCCATTAC 
TGCGAGAGTC TGCGGCCCCG GTGCTCCGGC TGCGATGAGA TAATATTCGC TGAGGACTAC 
CAGCGTGTGG AAGATCTGGC CTGGCACCGA AAGCACTTTG TCTGTGAGGG TTGTGAGCAG 
CTGCTGAGCG GCCGGGCGTA CATCGTCACC AAGGGTCAGC TTCTGTGCCC AACTTGCAGC 
AAGTCCAAAC GCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LMCD1 ( NM_014583.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055398.1 Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00589
Clone ID Related Accession (Same CDS sequence)	NM_014583.3 , NM_014583.4
Accession Version	NM_014583.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-29
Organism	Homo sapiens(human)
Product	LIM and cysteine-rich domains protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN388104.1, AF169284.1, BU619403.1 and AC087859.3. On May 17, 2019 this sequence version replaced NM_014583.3. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803614.63208.1, SRR1660805.150890.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000157600.8/ ENSP00000157600.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAAAGG TGGCTAAGGA CCTCAACCCA GGAGTTAAAA AGATGTCCCT GGGCCAGCTG 
CAGTCAGCAA GAGGTGTGGC ATGTTTGGGA TGCAAGGGGA CGTGTTCGGG CTTCGAGCCA 
CATTCATGGA GGAAAATATG CAAGTCTTGC AAATGCAGCC AAGAGGACCA CTGCCTAACA 
TCTGACCTAG AAGACGATCG GAAAATTGGC CGCTTGCTGA TGGACTCCAA GTATTCCACC 
CTCACTGCTC GGGTGAAAGG CGGGGACGGC ATCCGGATTT ACAAGAGGAA CCGGATGATC 
ATGACCAACC CTATTGCTAC TGGGAAAGAT CCCACTTTTG ACACCATCAC CTACGAGTGG 
GCTCCCCCTG GAGTCACCCA GAAACTGGGA CTGCAGTACA TGGAGCTCAT CCCCAAGGAG 
AAGCAGCCAG TGACAGGCAC AGAGGGTGCC TTTTACCGCC GCCGCCAGCT CATGCACCAG 
CTCCCCATCT ATGACCAGGA TCCCTCGCGC TGCCGTGGAC TTTTGGAGAA TGAGTTGAAA 
CTGATGGAAG AATTTGTCAA GCAATATAAG AGCGAGGCCC TCGGCGTGGG AGAAGTGGCC 
CTCCCGGGGC AGGGTGGCTT GCCCAAGGAG GAGGGGAAGC AGCAGGAAAA GCCAGAGGGG 
GCAGAGACCA CTGCTGCTAC CACCAACGGC AGTCTCAGTG ACCCGTCCAA AGAAGTGGAA 
TACGTCTGCG AGCTCTGCAA GGGAGCGGCC CCTCCTGACA GCCCCGTGGT CTACTCGGAC 
AGGGCAGGCT ACAACAAGCA GTGGCACCCC ACCTGCTTTG TGTGTGCCAA GTGCTCCGAG 
CCGCTGGTGG ACCTCATCTA CTTCTGGAAG GATGGTGCAC CCTGGTGCGG CCGCCATTAC 
TGCGAGAGTC TGCGGCCCCG GTGCTCCGGC TGCGATGAGA TAATATTCGC TGAGGACTAC 
CAGCGTGTGG AAGATCTGGC CTGGCACCGA AAGCACTTTG TCTGTGAGGG TTGTGAGCAG 
CTGCTGAGCG GCCGGGCGTA CATCGTCACC AAGGGTCAGC TTCTGTGCCC AACTTGCAGC 
AAGTCCAAAC GCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055398.1
CDS	117..1214
Translation	MAKVAKDLNPGVKKMSLGQLQSARGVACLGCKGTCSGFEPHSWRKICKSCKCSQEDHCLTSDLEDDRKIGRLLMDSKYSTLTARVKGGDGIRIYKRNRMIMTNPIATGKDPTFDTITYEWAPPGVTQKLGLQYMELIPKEKQPVTGTEGAFYRRRQLMHQLPIYDQDPSRCRGLLENELKLMEEFVKQYKSEALGVGEVALPGQGGLPKEEGKQQEKPEGAETTAATTNGSLSDPSKEVEYVCELCKGAAPPDSPVVYSDRAGYNKQWHPTCFVCAKCSEPLVDLIYFWKDGAPWCGRHYCESLRPRCSGCDEIIFAEDYQRVEDLAWHRKHFVCEGCEQLLSGRAYIVTKGQLLCPTCSKSKRS

Target ORF information:

RefSeq Version	NM_014583.4
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014583.4

ORF Insert Sequence:

ATGGCAAAGG TGGCTAAGGA CCTCAACCCA GGAGTTAAAA AGATGTCCCT GGGCCAGCTG 
CAGTCAGCAA GAGGTGTGGC ATGTTTGGGA TGCAAGGGGA CGTGTTCGGG CTTCGAGCCA 
CATTCATGGA GGAAAATATG CAAGTCTTGC AAATGCAGCC AAGAGGACCA CTGCCTAACA 
TCTGACCTAG AAGACGATCG GAAAATTGGC CGCTTGCTGA TGGACTCCAA GTATTCCACC 
CTCACTGCTC GGGTGAAAGG CGGGGACGGC ATCCGGATTT ACAAGAGGAA CCGGATGATC 
ATGACCAACC CTATTGCTAC TGGGAAAGAT CCCACTTTTG ACACCATCAC CTACGAGTGG 
GCTCCCCCTG GAGTCACCCA GAAACTGGGA CTGCAGTACA TGGAGCTCAT CCCCAAGGAG 
AAGCAGCCAG TGACAGGCAC AGAGGGTGCC TTTTACCGCC GCCGCCAGCT CATGCACCAG 
CTCCCCATCT ATGACCAGGA TCCCTCGCGC TGCCGTGGAC TTTTGGAGAA TGAGTTGAAA 
CTGATGGAAG AATTTGTCAA GCAATATAAG AGCGAGGCCC TCGGCGTGGG AGAAGTGGCC 
CTCCCGGGGC AGGGTGGCTT GCCCAAGGAG GAGGGGAAGC AGCAGGAAAA GCCAGAGGGG 
GCAGAGACCA CTGCTGCTAC CACCAACGGC AGTCTCAGTG ACCCGTCCAA AGAAGTGGAA 
TACGTCTGCG AGCTCTGCAA GGGAGCGGCC CCTCCTGACA GCCCCGTGGT CTACTCGGAC 
AGGGCAGGCT ACAACAAGCA GTGGCACCCC ACCTGCTTTG TGTGTGCCAA GTGCTCCGAG 
CCGCTGGTGG ACCTCATCTA CTTCTGGAAG GATGGTGCAC CCTGGTGCGG CCGCCATTAC 
TGCGAGAGTC TGCGGCCCCG GTGCTCCGGC TGCGATGAGA TAATATTCGC TGAGGACTAC 
CAGCGTGTGG AAGATCTGGC CTGGCACCGA AAGCACTTTG TCTGTGAGGG TTGTGAGCAG 
CTGCTGAGCG GCCGGGCGTA CATCGTCACC AAGGGTCAGC TTCTGTGCCC AACTTGCAGC 
AAGTCCAAAC GCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LMCD1 ( NM_001278233.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265162.1 Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04377
Clone ID Related Accession (Same CDS sequence)	NM_001278233.2 , NM_001278233.1
Accession Version	NM_001278233.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	LIM and cysteine-rich domains protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN388104.1, AK294455.1, BC000646.2, BU619403.1 and AC087859.3. On May 31, 2019 this sequence version replaced NM_001278233.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region and uses a downstream start codon compared to variant 1. The encoded isoform (2) is shorter at the N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.664460.1, SRR1660807.53915.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTCCA AGTATTCCAC CCTCACTGCT CGGGTGAAAG GCGGGGACGG CATCCGGATT 
TACAAGAGGA ACCGGATGAT CATGACCAAC CCTATTGCTA CTGGGAAAGA TCCCACTTTT 
GACACCATCA CCTACGAGTG GGCTCCCCCT GGAGTCACCC AGAAACTGGG ACTGCAGTAC 
ATGGAGCTCA TCCCCAAGGA GAAGCAGCCA GTGACAGGCA CAGAGGGTGC CTTTTACCGC 
CGCCGCCAGC TCATGCACCA GCTCCCCATC TATGACCAGG ATCCCTCGCG CTGCCGTGGA 
CTTTTGGAGA ATGAGTTGAA ACTGATGGAA GAATTTGTCA AGCAATATAA GAGCGAGGCC 
CTCGGCGTGG GAGAAGTGGC CCTCCCGGGG CAGGGTGGCT TGCCCAAGGA GGAGGGGAAG 
CAGCAGGAAA AGCCAGAGGG GGCAGAGACC ACTGCTGCTA CCACCAACGG CAGTCTCAGT 
GACCCGTCCA AAGAAGTGGA ATACGTCTGC GAGCTCTGCA AGGGAGCGGC CCCTCCTGAC 
AGCCCCGTGG TCTACTCGGA CAGGGCAGGC TACAACAAGC AGTGGCACCC CACCTGCTTT 
GTGTGTGCCA AGTGCTCCGA GCCGCTGGTG GACCTCATCT ACTTCTGGAA GGATGGTGCA 
CCCTGGTGCG GCCGCCATTA CTGCGAGAGT CTGCGGCCCC GGTGCTCCGG CTGCGATGAG 
ATAATATTCG CTGAGGACTA CCAGCGTGTG GAAGATCTGG CCTGGCACCG AAAGCACTTT 
GTCTGTGAGG GTTGTGAGCA GCTGCTGAGC GGCCGGGCGT ACATCGTCAC CAAGGGTCAG 
CTTCTGTGCC CAACTTGCAG CAAGTCCAAA CGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265162.1
CDS	247..1125
Translation	MDSKYSTLTARVKGGDGIRIYKRNRMIMTNPIATGKDPTFDTITYEWAPPGVTQKLGLQYMELIPKEKQPVTGTEGAFYRRRQLMHQLPIYDQDPSRCRGLLENELKLMEEFVKQYKSEALGVGEVALPGQGGLPKEEGKQQEKPEGAETTAATTNGSLSDPSKEVEYVCELCKGAAPPDSPVVYSDRAGYNKQWHPTCFVCAKCSEPLVDLIYFWKDGAPWCGRHYCESLRPRCSGCDEIIFAEDYQRVEDLAWHRKHFVCEGCEQLLSGRAYIVTKGQLLCPTCSKSKRS

Target ORF information:

RefSeq Version	NM_001278233.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278233.2

ORF Insert Sequence:

ATGGACTCCA AGTATTCCAC CCTCACTGCT CGGGTGAAAG GCGGGGACGG CATCCGGATT 
TACAAGAGGA ACCGGATGAT CATGACCAAC CCTATTGCTA CTGGGAAAGA TCCCACTTTT 
GACACCATCA CCTACGAGTG GGCTCCCCCT GGAGTCACCC AGAAACTGGG ACTGCAGTAC 
ATGGAGCTCA TCCCCAAGGA GAAGCAGCCA GTGACAGGCA CAGAGGGTGC CTTTTACCGC 
CGCCGCCAGC TCATGCACCA GCTCCCCATC TATGACCAGG ATCCCTCGCG CTGCCGTGGA 
CTTTTGGAGA ATGAGTTGAA ACTGATGGAA GAATTTGTCA AGCAATATAA GAGCGAGGCC 
CTCGGCGTGG GAGAAGTGGC CCTCCCGGGG CAGGGTGGCT TGCCCAAGGA GGAGGGGAAG 
CAGCAGGAAA AGCCAGAGGG GGCAGAGACC ACTGCTGCTA CCACCAACGG CAGTCTCAGT 
GACCCGTCCA AAGAAGTGGA ATACGTCTGC GAGCTCTGCA AGGGAGCGGC CCCTCCTGAC 
AGCCCCGTGG TCTACTCGGA CAGGGCAGGC TACAACAAGC AGTGGCACCC CACCTGCTTT 
GTGTGTGCCA AGTGCTCCGA GCCGCTGGTG GACCTCATCT ACTTCTGGAA GGATGGTGCA 
CCCTGGTGCG GCCGCCATTA CTGCGAGAGT CTGCGGCCCC GGTGCTCCGG CTGCGATGAG 
ATAATATTCG CTGAGGACTA CCAGCGTGTG GAAGATCTGG CCTGGCACCG AAAGCACTTT 
GTCTGTGAGG GTTGTGAGCA GCTGCTGAGC GGCCGGGCGT ACATCGTCAC CAAGGGTCAG 
CTTCTGTGCC CAACTTGCAG CAAGTCCAAA CGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LMCD1 ( NM_001278233.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265162.1 Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 2, mRNA.

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CloneID	OHu04377
Clone ID Related Accession (Same CDS sequence)	NM_001278233.2 , NM_001278233.1
Accession Version	NM_001278233.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-12
Organism	Homo sapiens(human)
Product	LIM and cysteine-rich domains protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN388104.1, AK294455.1, BC000646.2 and BU619403.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region and uses a downstream start codon compared to variant 1. The encoded isoform (2) is shorter at the N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.53915.1, AK294455.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTCCA AGTATTCCAC CCTCACTGCT CGGGTGAAAG GCGGGGACGG CATCCGGATT 
TACAAGAGGA ACCGGATGAT CATGACCAAC CCTATTGCTA CTGGGAAAGA TCCCACTTTT 
GACACCATCA CCTACGAGTG GGCTCCCCCT GGAGTCACCC AGAAACTGGG ACTGCAGTAC 
ATGGAGCTCA TCCCCAAGGA GAAGCAGCCA GTGACAGGCA CAGAGGGTGC CTTTTACCGC 
CGCCGCCAGC TCATGCACCA GCTCCCCATC TATGACCAGG ATCCCTCGCG CTGCCGTGGA 
CTTTTGGAGA ATGAGTTGAA ACTGATGGAA GAATTTGTCA AGCAATATAA GAGCGAGGCC 
CTCGGCGTGG GAGAAGTGGC CCTCCCGGGG CAGGGTGGCT TGCCCAAGGA GGAGGGGAAG 
CAGCAGGAAA AGCCAGAGGG GGCAGAGACC ACTGCTGCTA CCACCAACGG CAGTCTCAGT 
GACCCGTCCA AAGAAGTGGA ATACGTCTGC GAGCTCTGCA AGGGAGCGGC CCCTCCTGAC 
AGCCCCGTGG TCTACTCGGA CAGGGCAGGC TACAACAAGC AGTGGCACCC CACCTGCTTT 
GTGTGTGCCA AGTGCTCCGA GCCGCTGGTG GACCTCATCT ACTTCTGGAA GGATGGTGCA 
CCCTGGTGCG GCCGCCATTA CTGCGAGAGT CTGCGGCCCC GGTGCTCCGG CTGCGATGAG 
ATAATATTCG CTGAGGACTA CCAGCGTGTG GAAGATCTGG CCTGGCACCG AAAGCACTTT 
GTCTGTGAGG GTTGTGAGCA GCTGCTGAGC GGCCGGGCGT ACATCGTCAC CAAGGGTCAG 
CTTCTGTGCC CAACTTGCAG CAAGTCCAAA CGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265162.1
CDS	263..1141
Translation	MDSKYSTLTARVKGGDGIRIYKRNRMIMTNPIATGKDPTFDTITYEWAPPGVTQKLGLQYMELIPKEKQPVTGTEGAFYRRRQLMHQLPIYDQDPSRCRGLLENELKLMEEFVKQYKSEALGVGEVALPGQGGLPKEEGKQQEKPEGAETTAATTNGSLSDPSKEVEYVCELCKGAAPPDSPVVYSDRAGYNKQWHPTCFVCAKCSEPLVDLIYFWKDGAPWCGRHYCESLRPRCSGCDEIIFAEDYQRVEDLAWHRKHFVCEGCEQLLSGRAYIVTKGQLLCPTCSKSKRS

Target ORF information:

RefSeq Version	NM_001278233.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278233.1

ORF Insert Sequence:

ATGGACTCCA AGTATTCCAC CCTCACTGCT CGGGTGAAAG GCGGGGACGG CATCCGGATT 
TACAAGAGGA ACCGGATGAT CATGACCAAC CCTATTGCTA CTGGGAAAGA TCCCACTTTT 
GACACCATCA CCTACGAGTG GGCTCCCCCT GGAGTCACCC AGAAACTGGG ACTGCAGTAC 
ATGGAGCTCA TCCCCAAGGA GAAGCAGCCA GTGACAGGCA CAGAGGGTGC CTTTTACCGC 
CGCCGCCAGC TCATGCACCA GCTCCCCATC TATGACCAGG ATCCCTCGCG CTGCCGTGGA 
CTTTTGGAGA ATGAGTTGAA ACTGATGGAA GAATTTGTCA AGCAATATAA GAGCGAGGCC 
CTCGGCGTGG GAGAAGTGGC CCTCCCGGGG CAGGGTGGCT TGCCCAAGGA GGAGGGGAAG 
CAGCAGGAAA AGCCAGAGGG GGCAGAGACC ACTGCTGCTA CCACCAACGG CAGTCTCAGT 
GACCCGTCCA AAGAAGTGGA ATACGTCTGC GAGCTCTGCA AGGGAGCGGC CCCTCCTGAC 
AGCCCCGTGG TCTACTCGGA CAGGGCAGGC TACAACAAGC AGTGGCACCC CACCTGCTTT 
GTGTGTGCCA AGTGCTCCGA GCCGCTGGTG GACCTCATCT ACTTCTGGAA GGATGGTGCA 
CCCTGGTGCG GCCGCCATTA CTGCGAGAGT CTGCGGCCCC GGTGCTCCGG CTGCGATGAG 
ATAATATTCG CTGAGGACTA CCAGCGTGTG GAAGATCTGG CCTGGCACCG AAAGCACTTT 
GTCTGTGAGG GTTGTGAGCA GCTGCTGAGC GGCCGGGCGT ACATCGTCAC CAAGGGTCAG 
CTTCTGTGCC CAACTTGCAG CAAGTCCAAA CGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LMCD1 ( NM_001278234.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265163.1 Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu04378
Clone ID Related Accession (Same CDS sequence)	NM_001278234.1
Accession Version	NM_001278234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	LIM and cysteine-rich domains protein 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN388104.1, AK293849.1, BC000646.2 and BU619403.1. Transcript Variant: This variant (3) lacks an alternate exon and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.456270.1, AK293849.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGGGA TGCAAGGGGA CGTGTTCGGG CTTCGAGCCA CATTCATGGA GGGACTGCAG 
TACATGGAGC TCATCCCCAA GGAGAAGCAG CCAGTGACAG GCACAGAGGG TGCCTTTTAC 
CGCCGCCGCC AGCTCATGCA CCAGCTCCCC ATCTATGACC AGGATCCCTC GCGCTGCCGT 
GGACTTTTGG AGAATGAGTT GAAACTGATG GAAGAATTTG TCAAGCAATA TAAGAGCGAG 
GCCCTCGGCG TGGGAGAAGT GGCCCTCCCG GGGCAGGGTG GCTTGCCCAA GGAGGAGGGG 
AAGCAGCAGG AAAAGCCAGA GGGGGCAGAG ACCACTGCTG CTACCACCAA CGGCAGTCTC 
AGTGACCCGT CCAAAGAAGT GGAATACGTC TGCGAGCTCT GCAAGGGAGC GGCCCCTCCT 
GACAGCCCCG TGGTCTACTC GGACAGGGCA GGCTACAACA AGCAGTGGCA CCCCACCTGC 
TTTGTGTGTG CCAAGTGCTC CGAGCCGCTG GTGGACCTCA TCTACTTCTG GAAGGATGGT 
GCACCCTGGT GCGGCCGCCA TTACTGCGAG AGTCTGCGGC CCCGGTGCTC CGGCTGCGAT 
GAGATAATAT TCGCTGAGGA CTACCAGCGT GTGGAAGATC TGGCCTGGCA CCGAAAGCAC 
TTTGTCTGTG AGGGTTGTGA GCAGCTGCTG AGCGGCCGGG CGTACATCGT CACCAAGGGT 
CAGCTTCTGT GCCCAACTTG CAGCAAGTCC AAACGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265163.1
CDS	213..974
Translation	MFGMQGDVFGLRATFMEGLQYMELIPKEKQPVTGTEGAFYRRRQLMHQLPIYDQDPSRCRGLLENELKLMEEFVKQYKSEALGVGEVALPGQGGLPKEEGKQQEKPEGAETTAATTNGSLSDPSKEVEYVCELCKGAAPPDSPVVYSDRAGYNKQWHPTCFVCAKCSEPLVDLIYFWKDGAPWCGRHYCESLRPRCSGCDEIIFAEDYQRVEDLAWHRKHFVCEGCEQLLSGRAYIVTKGQLLCPTCSKSKRS

Target ORF information:

RefSeq Version	NM_001278234.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278234.1

ORF Insert Sequence:

ATGTTTGGGA TGCAAGGGGA CGTGTTCGGG CTTCGAGCCA CATTCATGGA GGGACTGCAG 
TACATGGAGC TCATCCCCAA GGAGAAGCAG CCAGTGACAG GCACAGAGGG TGCCTTTTAC 
CGCCGCCGCC AGCTCATGCA CCAGCTCCCC ATCTATGACC AGGATCCCTC GCGCTGCCGT 
GGACTTTTGG AGAATGAGTT GAAACTGATG GAAGAATTTG TCAAGCAATA TAAGAGCGAG 
GCCCTCGGCG TGGGAGAAGT GGCCCTCCCG GGGCAGGGTG GCTTGCCCAA GGAGGAGGGG 
AAGCAGCAGG AAAAGCCAGA GGGGGCAGAG ACCACTGCTG CTACCACCAA CGGCAGTCTC 
AGTGACCCGT CCAAAGAAGT GGAATACGTC TGCGAGCTCT GCAAGGGAGC GGCCCCTCCT 
GACAGCCCCG TGGTCTACTC GGACAGGGCA GGCTACAACA AGCAGTGGCA CCCCACCTGC 
TTTGTGTGTG CCAAGTGCTC CGAGCCGCTG GTGGACCTCA TCTACTTCTG GAAGGATGGT 
GCACCCTGGT GCGGCCGCCA TTACTGCGAG AGTCTGCGGC CCCGGTGCTC CGGCTGCGAT 
GAGATAATAT TCGCTGAGGA CTACCAGCGT GTGGAAGATC TGGCCTGGCA CCGAAAGCAC 
TTTGTCTGTG AGGGTTGTGA GCAGCTGCTG AGCGGCCGGG CGTACATCGT CACCAAGGGT 
CAGCTTCTGT GCCCAACTTG CAGCAAGTCC AAACGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LMCD1 ( NM_001278235.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265164.1 Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu04379
Clone ID Related Accession (Same CDS sequence)	NM_001278235.2 , NM_001278235.1
Accession Version	NM_001278235.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	LIM and cysteine-rich domains protein 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300803.1, BC000646.2, BX114807.1, AI693011.1, W47274.1, N95437.1 and AI951010.1. On May 31, 2019 this sequence version replaced NM_001278235.1. Transcript Variant: This variant (4) contains an alternate 3' terminal exon which results in an early stop codon, compared to variant 1. It encodes isoform 4, which has a shorter and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK300803.1, SRR6380200.67454.23 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAAGG TGGCTAAGGA CCTCAACCCA GGAGTTAAAA AGATGTCCCT GGGCCAGCTG 
CAGTCAGCAA GAGGTGTGGC ATGTTTGGGA TGCAAGGGGA CGTGTTCGGG CTTCGAGCCA 
CATTCATGGA GGAAAATATG CAAGTCTTGC AAATGCAGCC AAGAGGACCA CTGCCTAACA 
TCTGACCTAG AAGACGATCG GAAAATTGGC CGCTTGCTGA TGGACTCCAA GTATTCCACC 
CTCACTGCTC GGGTGAAAGG CGGGGACGGC ATCCGGATTT ACAAGAGGAA CCGGATGATC 
ATGACCAACC CTATTGCTAC TGGGAAAGAT CCCACTTTTG ACACCATCAC CTACGAGTGG 
GCTCCCCCTG GAGTCACCCA GAAACTGGGA CTGCAGTACA TGGAGCTCAT CCCCAAGGAG 
AAGCAGCCAG TGACAGGCAC AGAGGGTGCC TTTTACCGCC GCCGCCAGCT CATGCACCAG 
CTCCCCATCT ATGACCAGGA TCCCTCGCGC TGCCGTGGAC TTTTGGAGAA TGAGTTGAAA 
CTGATGGAAG AATTTGTCAA GCAATATAAG AGCGAGGCCC TCGGCGTGGG AGAAGTGGCC 
CTCCCGGGGC AGGGTGGCTT GCCCAAGGAG GAGGGGAAGC AGCAGGAAAA GCCAGAGGGG 
GCAGAGACCA CTGCTGCTAC CACCAACGGC AGTCTCAGTG ACCCGTCCAA AGAAGTGGAA 
TACAAGCCAG TCCCACTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265164.1
CDS	117..860
Translation	MAKVAKDLNPGVKKMSLGQLQSARGVACLGCKGTCSGFEPHSWRKICKSCKCSQEDHCLTSDLEDDRKIGRLLMDSKYSTLTARVKGGDGIRIYKRNRMIMTNPIATGKDPTFDTITYEWAPPGVTQKLGLQYMELIPKEKQPVTGTEGAFYRRRQLMHQLPIYDQDPSRCRGLLENELKLMEEFVKQYKSEALGVGEVALPGQGGLPKEEGKQQEKPEGAETTAATTNGSLSDPSKEVEYKPVPLV

Target ORF information:

RefSeq Version	NM_001278235.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278235.2

ORF Insert Sequence:

ATGGCAAAGG TGGCTAAGGA CCTCAACCCA GGAGTTAAAA AGATGTCCCT GGGCCAGCTG 
CAGTCAGCAA GAGGTGTGGC ATGTTTGGGA TGCAAGGGGA CGTGTTCGGG CTTCGAGCCA 
CATTCATGGA GGAAAATATG CAAGTCTTGC AAATGCAGCC AAGAGGACCA CTGCCTAACA 
TCTGACCTAG AAGACGATCG GAAAATTGGC CGCTTGCTGA TGGACTCCAA GTATTCCACC 
CTCACTGCTC GGGTGAAAGG CGGGGACGGC ATCCGGATTT ACAAGAGGAA CCGGATGATC 
ATGACCAACC CTATTGCTAC TGGGAAAGAT CCCACTTTTG ACACCATCAC CTACGAGTGG 
GCTCCCCCTG GAGTCACCCA GAAACTGGGA CTGCAGTACA TGGAGCTCAT CCCCAAGGAG 
AAGCAGCCAG TGACAGGCAC AGAGGGTGCC TTTTACCGCC GCCGCCAGCT CATGCACCAG 
CTCCCCATCT ATGACCAGGA TCCCTCGCGC TGCCGTGGAC TTTTGGAGAA TGAGTTGAAA 
CTGATGGAAG AATTTGTCAA GCAATATAAG AGCGAGGCCC TCGGCGTGGG AGAAGTGGCC 
CTCCCGGGGC AGGGTGGCTT GCCCAAGGAG GAGGGGAAGC AGCAGGAAAA GCCAGAGGGG 
GCAGAGACCA CTGCTGCTAC CACCAACGGC AGTCTCAGTG ACCCGTCCAA AGAAGTGGAA 
TACAAGCCAG TCCCACTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LMCD1 ( NM_001278235.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265164.1 Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu04379
Clone ID Related Accession (Same CDS sequence)	NM_001278235.2 , NM_001278235.1
Accession Version	NM_001278235.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	LIM and cysteine-rich domains protein 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN388104.1, AK300803.1, BC000646.2, BX114807.1, AI693011.1, W47274.1, N95437.1 and AI951010.1. Transcript Variant: This variant (4) contains an alternate 3' terminal exon which results in an early stop codon, compared to variant 1. It encodes isoform 4, which has a shorter and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK300803.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAAGG TGGCTAAGGA CCTCAACCCA GGAGTTAAAA AGATGTCCCT GGGCCAGCTG 
CAGTCAGCAA GAGGTGTGGC ATGTTTGGGA TGCAAGGGGA CGTGTTCGGG CTTCGAGCCA 
CATTCATGGA GGAAAATATG CAAGTCTTGC AAATGCAGCC AAGAGGACCA CTGCCTAACA 
TCTGACCTAG AAGACGATCG GAAAATTGGC CGCTTGCTGA TGGACTCCAA GTATTCCACC 
CTCACTGCTC GGGTGAAAGG CGGGGACGGC ATCCGGATTT ACAAGAGGAA CCGGATGATC 
ATGACCAACC CTATTGCTAC TGGGAAAGAT CCCACTTTTG ACACCATCAC CTACGAGTGG 
GCTCCCCCTG GAGTCACCCA GAAACTGGGA CTGCAGTACA TGGAGCTCAT CCCCAAGGAG 
AAGCAGCCAG TGACAGGCAC AGAGGGTGCC TTTTACCGCC GCCGCCAGCT CATGCACCAG 
CTCCCCATCT ATGACCAGGA TCCCTCGCGC TGCCGTGGAC TTTTGGAGAA TGAGTTGAAA 
CTGATGGAAG AATTTGTCAA GCAATATAAG AGCGAGGCCC TCGGCGTGGG AGAAGTGGCC 
CTCCCGGGGC AGGGTGGCTT GCCCAAGGAG GAGGGGAAGC AGCAGGAAAA GCCAGAGGGG 
GCAGAGACCA CTGCTGCTAC CACCAACGGC AGTCTCAGTG ACCCGTCCAA AGAAGTGGAA 
TACAAGCCAG TCCCACTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265164.1
CDS	133..876
Translation	MAKVAKDLNPGVKKMSLGQLQSARGVACLGCKGTCSGFEPHSWRKICKSCKCSQEDHCLTSDLEDDRKIGRLLMDSKYSTLTARVKGGDGIRIYKRNRMIMTNPIATGKDPTFDTITYEWAPPGVTQKLGLQYMELIPKEKQPVTGTEGAFYRRRQLMHQLPIYDQDPSRCRGLLENELKLMEEFVKQYKSEALGVGEVALPGQGGLPKEEGKQQEKPEGAETTAATTNGSLSDPSKEVEYKPVPLV

Target ORF information:

RefSeq Version	NM_001278235.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278235.1

ORF Insert Sequence:

ATGGCAAAGG TGGCTAAGGA CCTCAACCCA GGAGTTAAAA AGATGTCCCT GGGCCAGCTG 
CAGTCAGCAA GAGGTGTGGC ATGTTTGGGA TGCAAGGGGA CGTGTTCGGG CTTCGAGCCA 
CATTCATGGA GGAAAATATG CAAGTCTTGC AAATGCAGCC AAGAGGACCA CTGCCTAACA 
TCTGACCTAG AAGACGATCG GAAAATTGGC CGCTTGCTGA TGGACTCCAA GTATTCCACC 
CTCACTGCTC GGGTGAAAGG CGGGGACGGC ATCCGGATTT ACAAGAGGAA CCGGATGATC 
ATGACCAACC CTATTGCTAC TGGGAAAGAT CCCACTTTTG ACACCATCAC CTACGAGTGG 
GCTCCCCCTG GAGTCACCCA GAAACTGGGA CTGCAGTACA TGGAGCTCAT CCCCAAGGAG 
AAGCAGCCAG TGACAGGCAC AGAGGGTGCC TTTTACCGCC GCCGCCAGCT CATGCACCAG 
CTCCCCATCT ATGACCAGGA TCCCTCGCGC TGCCGTGGAC TTTTGGAGAA TGAGTTGAAA 
CTGATGGAAG AATTTGTCAA GCAATATAAG AGCGAGGCCC TCGGCGTGGG AGAAGTGGCC 
CTCCCGGGGC AGGGTGGCTT GCCCAAGGAG GAGGGGAAGC AGCAGGAAAA GCCAGAGGGG 
GCAGAGACCA CTGCTGCTAC CACCAACGGC AGTCTCAGTG ACCCGTCCAA AGAAGTGGAA 
TACAAGCCAG TCCCACTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LMCD1 ( XM_011533649.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531951.1 Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu67460
Clone ID Related Accession (Same CDS sequence)	XM_011533649.1
Accession Version	XM_011533649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	LIM and cysteine-rich domains protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATATA CATATAAAGT AATCGAGCGA ATACTGGAGA TTGGTGCATT TCGACCGCAT 
ATGTCCCTGG GCCAGCTGCA GTCAGCAAGA GGTGTGGCAT GTTTGGGATG CAAGGGGACG 
TGTTCGGGCT TCGAGCCACA TTCATGGAGG AAAATATGCA AGTCTTGCAA ATGCAGCCAA 
GAGGACCACT GCCTAACATC TGACCTAGAA GACGATCGGA AAATTGGCCG CTTGCTGATG 
GACTCCAAGT ATTCCACCCT CACTGCTCGG GTGAAAGGCG GGGACGGCAT CCGGATTTAC 
AAGAGGAACC GGATGATCAT GACCAACCCT ATTGCTACTG GGAAAGATCC CACTTTTGAC 
ACCATCACCT ACGAGTGGGC TCCCCCTGGA GTCACCCAGA AACTGGGACT GCAGTACATG 
GAGCTCATCC CCAAGGAGAA GCAGCCAGTG ACAGGCACAG AGGGTGCCTT TTACCGCCGC 
CGCCAGCTCA TGCACCAGCT CCCCATCTAT GACCAGGATC CCTCGCGCTG CCGTGGACTT 
TTGGAGAATG AGTTGAAACT GATGGAAGAA TTTGTCAAGC AATATAAGAG CGAGGCCCTC 
GGCGTGGGAG AAGTGGCCCT CCCGGGGCAG GGTGGCTTGC CCAAGGAGGA GGGGAAGCAG 
CAGGAAAAGC CAGAGGGGGC AGAGACCACT GCTGCTACCA CCAACGGCAG TCTCAGTGAC 
CCGTCCAAAG AAGTGGAATA CGTCTGCGAG CTCTGCAAGG GAGCGGCCCC TCCTGACAGC 
CCCGTGGTCT ACTCGGACAG GGCAGGCTAC AACAAGCAGT GGCACCCCAC CTGCTTTGTG 
TGTGCCAAGT GCTCCGAGCC GCTGGTGGAC CTCATCTACT TCTGGAAGGA TGGTGCACCC 
TGGTGCGGCC GCCATTACTG CGAGAGTCTG CGGCCCCGGT GCTCCGGCTG CGATGAGATA 
ATATTCGCTG AGGACTACCA GCGTGTGGAA GATCTGGCCT GGCACCGAAA GCACTTTGTC 
TGTGAGGGTT GTGAGCAGCT GCTGAGCGGC CGGGCGTACA TCGTCACCAA GGGTCAGCTT 
CTGTGCCCAA CTTGCAGCAA GTCCAAACGC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531951.1
CDS	170..1285
Translation	MGYTYKVIERILEIGAFRPHMSLGQLQSARGVACLGCKGTCSGFEPHSWRKICKSCKCSQEDHCLTSDLEDDRKIGRLLMDSKYSTLTARVKGGDGIRIYKRNRMIMTNPIATGKDPTFDTITYEWAPPGVTQKLGLQYMELIPKEKQPVTGTEGAFYRRRQLMHQLPIYDQDPSRCRGLLENELKLMEEFVKQYKSEALGVGEVALPGQGGLPKEEGKQQEKPEGAETTAATTNGSLSDPSKEVEYVCELCKGAAPPDSPVVYSDRAGYNKQWHPTCFVCAKCSEPLVDLIYFWKDGAPWCGRHYCESLRPRCSGCDEIIFAEDYQRVEDLAWHRKHFVCEGCEQLLSGRAYIVTKGQLLCPTCSKSKRS

Target ORF information:

RefSeq Version	XM_011533649.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM and cysteine rich domains 1 (LMCD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533649.1

ORF Insert Sequence:

ATGGGATATA CATATAAAGT AATCGAGCGA ATACTGGAGA TTGGTGCATT TCGACCGCAT 
ATGTCCCTGG GCCAGCTGCA GTCAGCAAGA GGTGTGGCAT GTTTGGGATG CAAGGGGACG 
TGTTCGGGCT TCGAGCCACA TTCATGGAGG AAAATATGCA AGTCTTGCAA ATGCAGCCAA 
GAGGACCACT GCCTAACATC TGACCTAGAA GACGATCGGA AAATTGGCCG CTTGCTGATG 
GACTCCAAGT ATTCCACCCT CACTGCTCGG GTGAAAGGCG GGGACGGCAT CCGGATTTAC 
AAGAGGAACC GGATGATCAT GACCAACCCT ATTGCTACTG GGAAAGATCC CACTTTTGAC 
ACCATCACCT ACGAGTGGGC TCCCCCTGGA GTCACCCAGA AACTGGGACT GCAGTACATG 
GAGCTCATCC CCAAGGAGAA GCAGCCAGTG ACAGGCACAG AGGGTGCCTT TTACCGCCGC 
CGCCAGCTCA TGCACCAGCT CCCCATCTAT GACCAGGATC CCTCGCGCTG CCGTGGACTT 
TTGGAGAATG AGTTGAAACT GATGGAAGAA TTTGTCAAGC AATATAAGAG CGAGGCCCTC 
GGCGTGGGAG AAGTGGCCCT CCCGGGGCAG GGTGGCTTGC CCAAGGAGGA GGGGAAGCAG 
CAGGAAAAGC CAGAGGGGGC AGAGACCACT GCTGCTACCA CCAACGGCAG TCTCAGTGAC 
CCGTCCAAAG AAGTGGAATA CGTCTGCGAG CTCTGCAAGG GAGCGGCCCC TCCTGACAGC 
CCCGTGGTCT ACTCGGACAG GGCAGGCTAC AACAAGCAGT GGCACCCCAC CTGCTTTGTG 
TGTGCCAAGT GCTCCGAGCC GCTGGTGGAC CTCATCTACT TCTGGAAGGA TGGTGCACCC 
TGGTGCGGCC GCCATTACTG CGAGAGTCTG CGGCCCCGGT GCTCCGGCTG CGATGAGATA 
ATATTCGCTG AGGACTACCA GCGTGTGGAA GATCTGGCCT GGCACCGAAA GCACTTTGTC 
TGTGAGGGTT GTGAGCAGCT GCTGAGCGGC CGGGCGTACA TCGTCACCAA GGGTCAGCTT 
CTGTGCCCAA CTTGCAGCAA GTCCAAACGC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LIM and cysteine-rich domains 1 is required for thrombin-induced smooth muscle cell proliferation and promotes atherogenesis.
The Journal of biological chemistry(2018 Jan)
Janjanam J,Zhang B,Mani AM,Singh NK,Traylor JG,Orr AW,Rao GN

A common variant association study reveals novel susceptibility loci for low HDL-cholesterol levels in ethnic Arabs.
Clinical genetics90(6)518-525(2016 Dec)
Wakil SM,Ram R,Muiya NP,Andres E,Mazhar N,Hagos S,Alshahid M,Meyer BF,Morahan G,Dzimiri N

Somatic LMCD1 mutations promoted cell migration and tumor metastasis in hepatocellular carcinoma.
Oncogene31(21)2640-52(2012 May)
Chang CY,Lin SC,Su WH,Ho CM,Jou YS

Image-based genome-wide siRNA screen identifies selective autophagy factors.
Nature480(7375)113-7(2011 Dec)
Orvedahl A,Sumpter R Jr,Xiao G,Ng A,Zou Z,Tang Y,Narimatsu M,Gilpin C,Sun Q,Roth M,Forst CV,Wrana JL,Zhang YE,Luby-Phelps K,Xavier RJ,Xie Y,Levine B

Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for GATA6.
PloS one6(5)e20389(2011)
Donati C,Marseglia G,Magi A,Serrat? S,Cencetti F,Bernacchioni C,Nannetti G,Benelli M,Brunelli S,Torricelli F,Cossu G,Bruni P

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LMCD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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