NRBF2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NRBF2
Entrez Gene ID	29982
Full Name	nuclear receptor binding factor 2
Synonyms	COPR,COPR1,COPR2,NRBF-2
General protein information	Preferred Names nuclear receptor binding factor 2 Names nuclear receptor-binding factor 2 comodulator of PPAR and RXR 1 comodulator of PPAR and RXR 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q21.3
Disorder MIM:	616477

mRNA and Protein(s)

mRNA	Protein	Name
NM_030759.5	NP_110386.2	nuclear receptor-binding factor 2 isoform 1
XM_006717810.4	XP_006717873.1	nuclear receptor-binding factor 2 isoform X1
XM_006717810.3	XP_006717873.1	nuclear receptor-binding factor 2 isoform X1
NM_030759.4	NP_110386.2	nuclear receptor-binding factor 2 isoform 1
NM_001282405.1	NP_001269334.1	nuclear receptor-binding factor 2 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NRBF2	XP_507815.1
Bos taurus (cattle)	NRBF2	NP_001071599.1
Gallus gallus (chicken)	NRBF2	NP_001186462.1
Homo sapiens (human)	NRBF2	NP_110386.2
Xenopus tropicalis (tropical clawed frog)	nrbf2	NP_001016183.1
Rattus norvegicus (Norway rat)	Nrbf2	NP_071522.1
Mus musculus (house mouse)	Nrbf2	NP_001031370.1
Rattus norvegicus (Norway rat)	LOC100363472	XP_002728120.1
	NRBF2	XP_001092137.1
Canis lupus familiaris (dog)	NRBF2	XP_536362.3
Rattus norvegicus (Norway rat)	RGD1560263	XP_574989.1
Danio rerio (zebrafish)	nrbf2b	NP_001002613.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

MTORC1-mediated NRBF2 phosphorylation functions as a switch for the class III PtdIns3K and autophagy.
Ma X, Zhang S, He L, Rong Y, Brier LW, Sun Q, Liu R, Fan W, Chen S, Yue Z, Kim J, Guan KL, Li D, Zhong Q
Autophagy13(3)592-607(2017 Mar)

NRBF2 is involved in the autophagic degradation process of APP-CTFs in Alzheimer disease models.
Yang C, Cai CZ, Song JX, Tan JQ, Durairajan SSK, Iyaswamy A, Wu MY, Chen LL, Yue Z, Li M, Lu JH
Autophagy13(12)2028-2040(2017)

Characterization of Atg38 and NRBF2, a fifth subunit of the autophagic Vps34/PIK3C3 complex.
Ohashi Y, Soler N, Garc?a Orteg?n M, Zhang L, Kirsten ML, Perisic O, Masson GR, Burke JE, Jakobi AJ, Apostolakis AA, Johnson CM, Ohashi M, Ktistakis NT, Sachse C, Williams RL
Autophagy12(11)2129-2144(2016 Nov)

Dynamics and architecture of the NRBF2-containing phosphatidylinositol 3-kinase complex I of autophagy.
Young LN, Cho K, Lawrence R, Zoncu R, Hurley JH
Proceedings of the National Academy of Sciences of the United States of America113(29)8224-9(2016 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study reveals NRBF2 as a critical molecular switch of PtdIns3K and autophagy activation, and its on/off state is precisely controlled by MTORC1 through phosphorylation.
Title: MTORC1-mediated NRBF2 phosphorylation functions as a switch for the class III PtdIns3K and autophagy.

Atg38 and its human ortholog NRBF2, accessory components of complex I consisting of Vps15-Vps34-Vps30/Atg6-Atg14 (yeast) and PIK3R4/VPS15-PIK3C3/VPS34-BECN1/Beclin 1-ATG14 (human), were characterized.
Title: Characterization of Atg38 and NRBF2, a fifth subunit of the autophagic Vps34/PIK3C3 complex.

Here we show that a putative fifth subunit, nuclear receptor binding factor 2 (NRBF2), is a tightly bound component of the class III phosphatidylinositol 3-kinase complex I that profoundly affects its activity and architecture. NRBF2 is a homodimer and drives the dimerization of the larger PI3KC3-C1 complex, with implications for the higher-order organization of the preautophagosomal structure.
Title: Dynamics and architecture of the NRBF2-containing phosphatidylinositol 3-kinase complex I of autophagy.

Polymorphisms in a putative enhancer at the 10q21.2 Breast Cancer Risk Locus regulate NRBF2 expression, implicating this gene in the etiology of breast cancer.
Title: Polymorphisms in a Putative Enhancer at the 10q21.2 Breast Cancer Risk Locus Regulate NRBF2 Expression.

Nrbf2 may interact with the Atg14L-containing Beclin 1-Vps34 protein complex to modulate protein-protein interactions within the complex.
Title: Nrbf2 protein suppresses autophagy by modulating Atg14L protein-containing Beclin 1-Vps34 complex architecture and reducing intracellular phosphatidylinositol-3 phosphate levels.

The following NRBF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NRBF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14193	NM_030759.5 Latest version!	Homo sapiens nuclear receptor binding factor 2 (NRBF2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu44250	XM_006717810.4 Latest version!	Homo sapiens nuclear receptor binding factor 2 (NRBF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu14377	NM_001282405.1 Latest version!	Homo sapiens nuclear receptor binding factor 2 (NRBF2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu44250	XM_006717810.3	Homo sapiens nuclear receptor binding factor 2 (NRBF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu14193	NM_030759.4	Homo sapiens nuclear receptor binding factor 2 (NRBF2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NRBF2 ( NM_030759.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_110386.2 Homo sapiens nuclear receptor binding factor 2 (NRBF2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14193
Clone ID Related Accession (Same CDS sequence)	NM_030759.5 , NM_030759.4
Accession Version	NM_030759.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	nuclear receptor-binding factor 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AU310836.1, BC011707.1 and BC001345.1. On Nov 22, 2018 this sequence version replaced NM_030759.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL136656.1, SRR3476690.1160841.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000277746.11/ ENSP00000277746.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAAGTAA TGGAAGGACC CCTCAACCTG GCTCATCAAC AGAGCAGACG AGCAGACCGT 
TTATTAGCTG CAGGCAAATA CGAAGAGGCT ATTTCTTGTC ACAAAAAGGC TGCAGCATAT 
CTTTCTGAAG CCATGAAGCT GACACAGTCA GAGCAGGCTC ATCTTTCACT GGAATTGCAA 
AGGGATAGCC ATATGAAACA GCTCCTCCTC ATCCAAGAGA GATGGAAAAG GGCCCAGCGT 
GAAGAAAGAT TGAAAGCCCA GCAGAACACA GACAAGGATG CAGCTGCCCA TCTTCAGACA 
TCTCACAAAC CCTCTGCAGA GGATGCAGAG GGCCAGAGTC CCCTTTCTCA GAAGTACAGC 
CCTTCCACAG AGAAATGCCT GCCTGAGATT CAGGGGATCT TTGACAGGGA TCCAGACACA 
CTACTTTATT TACTTCAGCA AAAGAGTGAG CCAGCAGAGC CATGTATTGG AAGCAAAGCC 
CCAAAAGATG ATAAAACAAT TATAGAGGAG CAGGCAACCA AAATTGCAGA TTTGAAGAGG 
CATGTGGAAT TCCTTGTGGC TGAGAATGAA AGATTAAGGA AAGAAAATAA ACAACTAAAG 
GCTGAAAAGG CCAGACTTCT AAAAGGTCCA ATAGAAAAGG AGCTGGATGT AGATGCTGAT 
TTTGTAGAAA CGTCAGAGTT ATGGAGCTTG CCACCACATG CAGAAACTGC TACAGCCTCC 
TCAACCTGGC AGAAGTTCGC AGCAAATACT GGGAAAGCCA AGGACATTCC AATCCCCAAT 
CTTCCTCCCT TGGATTTTCC ATCTCCAGAA CTTCCTCTTA TGGAGCTCTC TGAGGATATT 
CTGAAAGGAT TTATGAATAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_110386.2
CDS	144..1007
Translation	MEVMEGPLNLAHQQSRRADRLLAAGKYEEAISCHKKAAAYLSEAMKLTQSEQAHLSLELQRDSHMKQLLLIQERWKRAQREERLKAQQNTDKDAAAHLQTSHKPSAEDAEGQSPLSQKYSPSTEKCLPEIQGIFDRDPDTLLYLLQQKSEPAEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGPIEKELDVDADFVETSELWSLPPHAETATASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGFMNN

Target ORF information:

RefSeq Version	NM_030759.5
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear receptor binding factor 2 (NRBF2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030759.5

ORF Insert Sequence:

ATGGAAGTAA TGGAAGGACC CCTCAACCTG GCTCATCAAC AGAGCAGACG AGCAGACCGT 
TTATTAGCTG CAGGCAAATA CGAAGAGGCT ATTTCTTGTC ACAAAAAGGC TGCAGCATAT 
CTTTCTGAAG CCATGAAGCT GACACAGTCA GAGCAGGCTC ATCTTTCACT GGAATTGCAA 
AGGGATAGCC ATATGAAACA GCTCCTCCTC ATCCAAGAGA GATGGAAAAG GGCCCAGCGT 
GAAGAAAGAT TGAAAGCCCA GCAGAACACA GACAAGGATG CAGCTGCCCA TCTTCAGACA 
TCTCACAAAC CCTCTGCAGA GGATGCAGAG GGCCAGAGTC CCCTTTCTCA GAAGTACAGC 
CCTTCCACAG AGAAATGCCT GCCTGAGATT CAGGGGATCT TTGACAGGGA TCCAGACACA 
CTACTTTATT TACTTCAGCA AAAGAGTGAG CCAGCAGAGC CATGTATTGG AAGCAAAGCC 
CCAAAAGATG ATAAAACAAT TATAGAGGAG CAGGCAACCA AAATTGCAGA TTTGAAGAGG 
CATGTGGAAT TCCTTGTGGC TGAGAATGAA AGATTAAGGA AAGAAAATAA ACAACTAAAG 
GCTGAAAAGG CCAGACTTCT AAAAGGTCCA ATAGAAAAGG AGCTGGATGT AGATGCTGAT 
TTTGTAGAAA CGTCAGAGTT ATGGAGCTTG CCACCACATG CAGAAACTGC TACAGCCTCC 
TCAACCTGGC AGAAGTTCGC AGCAAATACT GGGAAAGCCA AGGACATTCC AATCCCCAAT 
CTTCCTCCCT TGGATTTTCC ATCTCCAGAA CTTCCTCTTA TGGAGCTCTC TGAGGATATT 
CTGAAAGGAT TTATGAATAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRBF2 ( NM_030759.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_110386.2 Homo sapiens nuclear receptor binding factor 2 (NRBF2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14193
Clone ID Related Accession (Same CDS sequence)	NM_030759.5 , NM_030759.4
Accession Version	NM_030759.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	nuclear receptor-binding factor 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AU310836.1, BC011707.1, BC001345.1 and DB522359.1. On Sep 5, 2013 this sequence version replaced NM_030759.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AU310836.1, SRR1660807.11217.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGTAA TGGAAGGACC CCTCAACCTG GCTCATCAAC AGAGCAGACG AGCAGACCGT 
TTATTAGCTG CAGGCAAATA CGAAGAGGCT ATTTCTTGTC ACAAAAAGGC TGCAGCATAT 
CTTTCTGAAG CCATGAAGCT GACACAGTCA GAGCAGGCTC ATCTTTCACT GGAATTGCAA 
AGGGATAGCC ATATGAAACA GCTCCTCCTC ATCCAAGAGA GATGGAAAAG GGCCCAGCGT 
GAAGAAAGAT TGAAAGCCCA GCAGAACACA GACAAGGATG CAGCTGCCCA TCTTCAGACA 
TCTCACAAAC CCTCTGCAGA GGATGCAGAG GGCCAGAGTC CCCTTTCTCA GAAGTACAGC 
CCTTCCACAG AGAAATGCCT GCCTGAGATT CAGGGGATCT TTGACAGGGA TCCAGACACA 
CTACTTTATT TACTTCAGCA AAAGAGTGAG CCAGCAGAGC CATGTATTGG AAGCAAAGCC 
CCAAAAGATG ATAAAACAAT TATAGAGGAG CAGGCAACCA AAATTGCAGA TTTGAAGAGG 
CATGTGGAAT TCCTTGTGGC TGAGAATGAA AGATTAAGGA AAGAAAATAA ACAACTAAAG 
GCTGAAAAGG CCAGACTTCT AAAAGGTCCA ATAGAAAAGG AGCTGGATGT AGATGCTGAT 
TTTGTAGAAA CGTCAGAGTT ATGGAGCTTG CCACCACATG CAGAAACTGC TACAGCCTCC 
TCAACCTGGC AGAAGTTCGC AGCAAATACT GGGAAAGCCA AGGACATTCC AATCCCCAAT 
CTTCCTCCCT TGGATTTTCC ATCTCCAGAA CTTCCTCTTA TGGAGCTCTC TGAGGATATT 
CTGAAAGGAT TTATGAATAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_110386.2
CDS	225..1088
Translation	MEVMEGPLNLAHQQSRRADRLLAAGKYEEAISCHKKAAAYLSEAMKLTQSEQAHLSLELQRDSHMKQLLLIQERWKRAQREERLKAQQNTDKDAAAHLQTSHKPSAEDAEGQSPLSQKYSPSTEKCLPEIQGIFDRDPDTLLYLLQQKSEPAEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGPIEKELDVDADFVETSELWSLPPHAETATASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGFMNN

Target ORF information:

RefSeq Version	NM_030759.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear receptor binding factor 2 (NRBF2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030759.4

ORF Insert Sequence:

ATGGAAGTAA TGGAAGGACC CCTCAACCTG GCTCATCAAC AGAGCAGACG AGCAGACCGT 
TTATTAGCTG CAGGCAAATA CGAAGAGGCT ATTTCTTGTC ACAAAAAGGC TGCAGCATAT 
CTTTCTGAAG CCATGAAGCT GACACAGTCA GAGCAGGCTC ATCTTTCACT GGAATTGCAA 
AGGGATAGCC ATATGAAACA GCTCCTCCTC ATCCAAGAGA GATGGAAAAG GGCCCAGCGT 
GAAGAAAGAT TGAAAGCCCA GCAGAACACA GACAAGGATG CAGCTGCCCA TCTTCAGACA 
TCTCACAAAC CCTCTGCAGA GGATGCAGAG GGCCAGAGTC CCCTTTCTCA GAAGTACAGC 
CCTTCCACAG AGAAATGCCT GCCTGAGATT CAGGGGATCT TTGACAGGGA TCCAGACACA 
CTACTTTATT TACTTCAGCA AAAGAGTGAG CCAGCAGAGC CATGTATTGG AAGCAAAGCC 
CCAAAAGATG ATAAAACAAT TATAGAGGAG CAGGCAACCA AAATTGCAGA TTTGAAGAGG 
CATGTGGAAT TCCTTGTGGC TGAGAATGAA AGATTAAGGA AAGAAAATAA ACAACTAAAG 
GCTGAAAAGG CCAGACTTCT AAAAGGTCCA ATAGAAAAGG AGCTGGATGT AGATGCTGAT 
TTTGTAGAAA CGTCAGAGTT ATGGAGCTTG CCACCACATG CAGAAACTGC TACAGCCTCC 
TCAACCTGGC AGAAGTTCGC AGCAAATACT GGGAAAGCCA AGGACATTCC AATCCCCAAT 
CTTCCTCCCT TGGATTTTCC ATCTCCAGAA CTTCCTCTTA TGGAGCTCTC TGAGGATATT 
CTGAAAGGAT TTATGAATAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRBF2 ( XM_006717810.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717873.1 Homo sapiens nuclear receptor binding factor 2 (NRBF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu44250
Clone ID Related Accession (Same CDS sequence)	XM_006717810.4 , XM_006717810.3
Accession Version	XM_006717810.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nuclear receptor-binding factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006717810.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA CACAGTCAGA GCAGGCTCAT CTTTCACTGG AATTGCAAAG GGATAGCCAT 
ATGAAACAGC TCCTCCTCAT CCAAGAGAGA TGGAAAAGGG CCCAGCGTGA AGAAAGATTG 
AAAGCCCAGC AGAACACAGA CAAGGATGCA GCTGCCCATC TTCAGACATC TCACAAACCC 
TCTGCAGAGG ATGCAGAGGG CCAGAGTCCC CTTTCTCAGA AGTACAGCCC TTCCACAGAG 
AAATGCCTGC CTGAGATTCA GGGGATCTTT GACAGGGATC CAGACACACT ACTTTATTTA 
CTTCAGCAAA AGAGTGAGCC AGCAGAGCCA TGTATTGGAA GCAAAGCCCC AAAAGATGAT 
AAAACAATTA TAGAGGAGCA GGCAACCAAA ATTGCAGATT TGAAGAGGCA TGTGGAATTC 
CTTGTGGCTG AGAATGAAAG ATTAAGGAAA GAAAATAAAC AACTAAAGGC TGAAAAGGCC 
AGACTTCTAA AAGGTCCAAT AGAAAAGGAG CTGGATGTAG ATGCTGATTT TGTAGAAACG 
TCAGAGTTAT GGAGCTTGCC ACCACATGCA GAAACTGCTA CAGCCTCCTC AACCTGGCAG 
AAGTTCGCAG CAAATACTGG GAAAGCCAAG GACATTCCAA TCCCCAATCT TCCTCCCTTG 
GATTTTCCAT CTCCAGAACT TCCTCTTATG GAGCTCTCTG AGGATATTCT GAAAGGATTT 
ATGAATAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717873.1
CDS	502..1233
Translation	MKLTQSEQAHLSLELQRDSHMKQLLLIQERWKRAQREERLKAQQNTDKDAAAHLQTSHKPSAEDAEGQSPLSQKYSPSTEKCLPEIQGIFDRDPDTLLYLLQQKSEPAEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGPIEKELDVDADFVETSELWSLPPHAETATASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGFMNN

Target ORF information:

RefSeq Version	XM_006717810.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear receptor binding factor 2 (NRBF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717810.4

ORF Insert Sequence:

ATGAAGCTGA CACAGTCAGA GCAGGCTCAT CTTTCACTGG AATTGCAAAG GGATAGCCAT 
ATGAAACAGC TCCTCCTCAT CCAAGAGAGA TGGAAAAGGG CCCAGCGTGA AGAAAGATTG 
AAAGCCCAGC AGAACACAGA CAAGGATGCA GCTGCCCATC TTCAGACATC TCACAAACCC 
TCTGCAGAGG ATGCAGAGGG CCAGAGTCCC CTTTCTCAGA AGTACAGCCC TTCCACAGAG 
AAATGCCTGC CTGAGATTCA GGGGATCTTT GACAGGGATC CAGACACACT ACTTTATTTA 
CTTCAGCAAA AGAGTGAGCC AGCAGAGCCA TGTATTGGAA GCAAAGCCCC AAAAGATGAT 
AAAACAATTA TAGAGGAGCA GGCAACCAAA ATTGCAGATT TGAAGAGGCA TGTGGAATTC 
CTTGTGGCTG AGAATGAAAG ATTAAGGAAA GAAAATAAAC AACTAAAGGC TGAAAAGGCC 
AGACTTCTAA AAGGTCCAAT AGAAAAGGAG CTGGATGTAG ATGCTGATTT TGTAGAAACG 
TCAGAGTTAT GGAGCTTGCC ACCACATGCA GAAACTGCTA CAGCCTCCTC AACCTGGCAG 
AAGTTCGCAG CAAATACTGG GAAAGCCAAG GACATTCCAA TCCCCAATCT TCCTCCCTTG 
GATTTTCCAT CTCCAGAACT TCCTCTTATG GAGCTCTCTG AGGATATTCT GAAAGGATTT 
ATGAATAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRBF2 ( XM_006717810.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717873.1 Homo sapiens nuclear receptor binding factor 2 (NRBF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu44250
Clone ID Related Accession (Same CDS sequence)	XM_006717810.4 , XM_006717810.3
Accession Version	XM_006717810.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear receptor-binding factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717810.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA CACAGTCAGA GCAGGCTCAT CTTTCACTGG AATTGCAAAG GGATAGCCAT 
ATGAAACAGC TCCTCCTCAT CCAAGAGAGA TGGAAAAGGG CCCAGCGTGA AGAAAGATTG 
AAAGCCCAGC AGAACACAGA CAAGGATGCA GCTGCCCATC TTCAGACATC TCACAAACCC 
TCTGCAGAGG ATGCAGAGGG CCAGAGTCCC CTTTCTCAGA AGTACAGCCC TTCCACAGAG 
AAATGCCTGC CTGAGATTCA GGGGATCTTT GACAGGGATC CAGACACACT ACTTTATTTA 
CTTCAGCAAA AGAGTGAGCC AGCAGAGCCA TGTATTGGAA GCAAAGCCCC AAAAGATGAT 
AAAACAATTA TAGAGGAGCA GGCAACCAAA ATTGCAGATT TGAAGAGGCA TGTGGAATTC 
CTTGTGGCTG AGAATGAAAG ATTAAGGAAA GAAAATAAAC AACTAAAGGC TGAAAAGGCC 
AGACTTCTAA AAGGTCCAAT AGAAAAGGAG CTGGATGTAG ATGCTGATTT TGTAGAAACG 
TCAGAGTTAT GGAGCTTGCC ACCACATGCA GAAACTGCTA CAGCCTCCTC AACCTGGCAG 
AAGTTCGCAG CAAATACTGG GAAAGCCAAG GACATTCCAA TCCCCAATCT TCCTCCCTTG 
GATTTTCCAT CTCCAGAACT TCCTCTTATG GAGCTCTCTG AGGATATTCT GAAAGGATTT 
ATGAATAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717873.1
CDS	542..1273
Translation	MKLTQSEQAHLSLELQRDSHMKQLLLIQERWKRAQREERLKAQQNTDKDAAAHLQTSHKPSAEDAEGQSPLSQKYSPSTEKCLPEIQGIFDRDPDTLLYLLQQKSEPAEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGPIEKELDVDADFVETSELWSLPPHAETATASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGFMNN

Target ORF information:

RefSeq Version	XM_006717810.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear receptor binding factor 2 (NRBF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717810.3

ORF Insert Sequence:

ATGAAGCTGA CACAGTCAGA GCAGGCTCAT CTTTCACTGG AATTGCAAAG GGATAGCCAT 
ATGAAACAGC TCCTCCTCAT CCAAGAGAGA TGGAAAAGGG CCCAGCGTGA AGAAAGATTG 
AAAGCCCAGC AGAACACAGA CAAGGATGCA GCTGCCCATC TTCAGACATC TCACAAACCC 
TCTGCAGAGG ATGCAGAGGG CCAGAGTCCC CTTTCTCAGA AGTACAGCCC TTCCACAGAG 
AAATGCCTGC CTGAGATTCA GGGGATCTTT GACAGGGATC CAGACACACT ACTTTATTTA 
CTTCAGCAAA AGAGTGAGCC AGCAGAGCCA TGTATTGGAA GCAAAGCCCC AAAAGATGAT 
AAAACAATTA TAGAGGAGCA GGCAACCAAA ATTGCAGATT TGAAGAGGCA TGTGGAATTC 
CTTGTGGCTG AGAATGAAAG ATTAAGGAAA GAAAATAAAC AACTAAAGGC TGAAAAGGCC 
AGACTTCTAA AAGGTCCAAT AGAAAAGGAG CTGGATGTAG ATGCTGATTT TGTAGAAACG 
TCAGAGTTAT GGAGCTTGCC ACCACATGCA GAAACTGCTA CAGCCTCCTC AACCTGGCAG 
AAGTTCGCAG CAAATACTGG GAAAGCCAAG GACATTCCAA TCCCCAATCT TCCTCCCTTG 
GATTTTCCAT CTCCAGAACT TCCTCTTATG GAGCTCTCTG AGGATATTCT GAAAGGATTT 
ATGAATAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRBF2 ( NM_001282405.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269334.1 Homo sapiens nuclear receptor binding factor 2 (NRBF2), transcript variant 2, mRNA.

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CloneID	OHu14377
Clone ID Related Accession (Same CDS sequence)	NM_001282405.1
Accession Version	NM_001282405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	nuclear receptor-binding factor 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AU310836.1, AK301656.1, BC001345.1 and DB522359.1. On Sep 5, 2013 this sequence version replaced XM_005269721.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region which results in the use of an alternate AUG compared to variant 1. It encodes isoform 2 which is shorter and has a distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.2079.1, AK301656.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGTTCCCCG GCGCCACTAC TCCCCTTCCT AAGGCCGCCG CTTACCCCGG GGTCTATGGA 
AGTAATGGAA GGACCCCTCA ACCTGCATAT CTTTCTGAAG CCATGAAGCT GACACAGTCA 
GAGCAGGCTC ATCTTTCACT GGAATTGCAA AGGGATAGCC ATATGAAACA GCTCCTCCTC 
ATCCAAGAGA GATGGAAAAG GGCCCAGCGT GAAGAAAGAT TGAAAGCCCA GCAGAACACA 
GACAAGGATG CAGCTGCCCA TCTTCAGACA TCTCACAAAC CCTCTGCAGA GGATGCAGAG 
GGCCAGAGTC CCCTTTCTCA GAAGTACAGC CCTTCCACAG AGAAATGCCT GCCTGAGATT 
CAGGGGATCT TTGACAGGGA TCCAGACACA CTACTTTATT TACTTCAGCA AAAGAGTGAG 
CCAGCAGAGC CATGTATTGG AAGCAAAGCC CCAAAAGATG ATAAAACAAT TATAGAGGAG 
CAGGCAACCA AAATTGCAGA TTTGAAGAGG CATGTGGAAT TCCTTGTGGC TGAGAATGAA 
AGATTAAGGA AAGAAAATAA ACAACTAAAG GCTGAAAAGG CCAGACTTCT AAAAGGTCCA 
ATAGAAAAGG AGCTGGATGT AGATGCTGAT TTTGTAGAAA CGTCAGAGTT ATGGAGCTTG 
CCACCACATG CAGAAACTGC TACAGCCTCC TCAACCTGGC AGAAGTTCGC AGCAAATACT 
GGGAAAGCCA AGGACATTCC AATCCCCAAT CTTCCTCCCT TGGATTTTCC ATCTCCAGAA 
CTTCCTCTTA TGGAGCTCTC TGAGGATATT CTGAAAGGAT TTATGAATAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269334.1
CDS	170..1003
Translation	MFPGATTPLPKAAAYPGVYGSNGRTPQPAYLSEAMKLTQSEQAHLSLELQRDSHMKQLLLIQERWKRAQREERLKAQQNTDKDAAAHLQTSHKPSAEDAEGQSPLSQKYSPSTEKCLPEIQGIFDRDPDTLLYLLQQKSEPAEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGPIEKELDVDADFVETSELWSLPPHAETATASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGFMNN

Target ORF information:

RefSeq Version	NM_001282405.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear receptor binding factor 2 (NRBF2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282405.1

ORF Insert Sequence:

ATGTTCCCCG GCGCCACTAC TCCCCTTCCT AAGGCCGCCG CTTACCCCGG GGTCTATGGA 
AGTAATGGAA GGACCCCTCA ACCTGCATAT CTTTCTGAAG CCATGAAGCT GACACAGTCA 
GAGCAGGCTC ATCTTTCACT GGAATTGCAA AGGGATAGCC ATATGAAACA GCTCCTCCTC 
ATCCAAGAGA GATGGAAAAG GGCCCAGCGT GAAGAAAGAT TGAAAGCCCA GCAGAACACA 
GACAAGGATG CAGCTGCCCA TCTTCAGACA TCTCACAAAC CCTCTGCAGA GGATGCAGAG 
GGCCAGAGTC CCCTTTCTCA GAAGTACAGC CCTTCCACAG AGAAATGCCT GCCTGAGATT 
CAGGGGATCT TTGACAGGGA TCCAGACACA CTACTTTATT TACTTCAGCA AAAGAGTGAG 
CCAGCAGAGC CATGTATTGG AAGCAAAGCC CCAAAAGATG ATAAAACAAT TATAGAGGAG 
CAGGCAACCA AAATTGCAGA TTTGAAGAGG CATGTGGAAT TCCTTGTGGC TGAGAATGAA 
AGATTAAGGA AAGAAAATAA ACAACTAAAG GCTGAAAAGG CCAGACTTCT AAAAGGTCCA 
ATAGAAAAGG AGCTGGATGT AGATGCTGAT TTTGTAGAAA CGTCAGAGTT ATGGAGCTTG 
CCACCACATG CAGAAACTGC TACAGCCTCC TCAACCTGGC AGAAGTTCGC AGCAAATACT 
GGGAAAGCCA AGGACATTCC AATCCCCAAT CTTCCTCCCT TGGATTTTCC ATCTCCAGAA 
CTTCCTCTTA TGGAGCTCTC TGAGGATATT CTGAAAGGAT TTATGAATAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

MTORC1-mediated NRBF2 phosphorylation functions as a switch for the class III PtdIns3K and autophagy.
Autophagy13(3)592-607(2017 Mar)
Ma X,Zhang S,He L,Rong Y,Brier LW,Sun Q,Liu R,Fan W,Chen S,Yue Z,Kim J,Guan KL,Li D,Zhong Q

NRBF2 is involved in the autophagic degradation process of APP-CTFs in Alzheimer disease models.
Autophagy13(12)2028-2040(2017)
Yang C,Cai CZ,Song JX,Tan JQ,Durairajan SSK,Iyaswamy A,Wu MY,Chen LL,Yue Z,Li M,Lu JH

Characterization of Atg38 and NRBF2, a fifth subunit of the autophagic Vps34/PIK3C3 complex.
Autophagy12(11)2129-2144(2016 Nov)
Ohashi Y,Soler N,Garc?a Orteg?n M,Zhang L,Kirsten ML,Perisic O,Masson GR,Burke JE,Jakobi AJ,Apostolakis AA,Johnson CM,Ohashi M,Ktistakis NT,Sachse C,Williams RL

Dynamics and architecture of the NRBF2-containing phosphatidylinositol 3-kinase complex I of autophagy.
Proceedings of the National Academy of Sciences of the United States of America113(29)8224-9(2016 07)
Young LN,Cho K,Lawrence R,Zoncu R,Hurley JH

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NRBF2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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