DMGDH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DMGDH
Entrez Gene ID	29958
Full Name	dimethylglycine dehydrogenase
Synonyms	DMGDHD,ME2GLYDH
General protein information	Preferred Names dimethylglycine dehydrogenase Names dimethylglycine dehydrogenase, mitochondrial
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q14.1
Summary	This gene encodes an enzyme involved in the catabolism of choline, catalyzing the oxidative demethylation of dimethylglycine to form sarcosine. The enzyme is found as a monomer in the mitochondrial matrix, and uses flavin adenine dinucleotide and folate as cofactors. Mutation in this gene causes dimethylglycine dehydrogenase deficiency, characterized by a fishlike body odor, chronic muscle fatigue, and elevated levels of the muscle form of creatine kinase in serum. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013].
Disorder MIM:	605849
Disorder Html:	Dimethylglycine dehydrogenase deficiency, 605850 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011543355.2	XP_011541657.1	dimethylglycine dehydrogenase, mitochondrial isoform X2
XM_011543354.2	XP_011541656.1	dimethylglycine dehydrogenase, mitochondrial isoform X2
NM_013391.3	NP_037523.2	dimethylglycine dehydrogenase, mitochondrial precursor
XM_006714597.2	XP_006714660.1	dimethylglycine dehydrogenase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dmgdh	NP_083048.1
Danio rerio (zebrafish)	dmgdh	XP_002665871.2
Pan troglodytes (chimpanzee)	DMGDH	XP_526883.2
Canis lupus familiaris (dog)	DMGDH	XP_005618235.1
Gallus gallus (chicken)	DMGDH	XP_003643002.1
Caenorhabditis elegans (roundworm)	Y37E3.17	NP_001021746.1
Xenopus tropicalis (tropical clawed frog)	dmgdh	XP_002940239.2
Homo sapiens (human)	DMGDH	NP_037523.2
Rattus norvegicus (Norway rat)	Dmgdh	NP_620802.2
Bos taurus (cattle)	DMGDH	NP_001192474.1

Gene Ontology
Bibliography

Related articles in PubMed

Evidence of interaction between genes in the folate/homocysteine metabolic pathway in controlling risk of nonsyndromic oral cleft.
Wang P, Wu T, Schwender H, Wang H, Shi B, Wang Z, Yuan Y, Liu D, Wang M, Li J, Zhou Z, Zhu H, Beaty TH
Oral diseases(2018 Jan)

Nonalcoholic steatohepatitis is associated with a state of betaine-insufficiency.
Sookoian S, Puri P, Casta?o GO, Scian R, Mirshahi F, Sanyal AJ, Pirola CJ
Liver international : official journal of the International Association for the Study of the Liver37(4)611-619(2017 Apr)

Structure and biochemical properties of recombinant human dimethylglycine dehydrogenase and comparison to the disease-related H109R variant.
Augustin P, Hromic A, Pavkov-Keller T, Gruber K, Macheroux P
The FEBS journal283(19)3587-3603(2016 10)

Genetic polymorphisms that affect selenium status and response to selenium supplementation in United Kingdom pregnant women.
Mao J, Vanderlelie JJ, Perkins AV, Redman CW, Ahmadi KR, Rayman MP
The American journal of clinical nutrition103(1)100-6(2016 Jan)

Dimethylglycine Deficiency and the Development of Diabetes.
Magnusson M, Wang TJ, Clish C, Engstr?m G, Nilsson P, Gerszten RE, Melander O
Diabetes64(8)3010-6(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In nonalcoholic fatty liver disease patient, the missense variant p.Ser646Pro (rs1805074) in DMGDH gene was significantly associated with disease severity and circulating levels of dimethylglycine.
Title: Nonalcoholic steatohepatitis is associated with a state of betaine-insufficiency.

The structure-based analysis provided new insights into the kinetic properties of dimethylglycine dehydrogenase in particular with respect to oxygen reactivity.
Title: Structure and biochemical properties of recombinant human dimethylglycine dehydrogenase and comparison to the disease-related H109R variant.

In agreement with previous studies, we show that the genetic variant rs921943 in DMGDH is significantly associated with selenium status in United Kingdom pregnant women.
Title: Genetic polymorphisms that affect selenium status and response to selenium supplementation in United Kingdom pregnant women.

Genetic variation of DMGDH was associated with higher plasma insulin, increased insulin resistance and increased risk of incident diabetes.
Title: Dimethylglycine Deficiency and the Development of Diabetes.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following DMGDH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DMGDH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21255	NM_013391.3 Latest version!	Homo sapiens dimethylglycine dehydrogenase (DMGDH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu39555	XM_006714597.2 Latest version!	Homo sapiens dimethylglycine dehydrogenase (DMGDH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu67433	XM_011543354.2 Latest version!	Homo sapiens dimethylglycine dehydrogenase (DMGDH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu67434	XM_011543355.2 Latest version!	Homo sapiens dimethylglycine dehydrogenase (DMGDH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DMGDH ( NM_013391.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_037523.2 Homo sapiens dimethylglycine dehydrogenase (DMGDH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21255
Clone ID Related Accession (Same CDS sequence)	NM_013391.3
Accession Version	NM_013391.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Homo sapiens(human)
Product	dimethylglycine dehydrogenase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008502.8, BP276968.1, AF111858.1, AK057641.1, CA397896.1 and AA676742.1. This sequence is a reference standard in the RefSeqGene project. On Jul 24, 2013 this sequence version replaced NM_013391.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the supported protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK057641.1, AK314736.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000255189.8/ ENSP00000255189.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTCCGTC CCGGCGCGCA GCTGCTGCGG GGCCTCCTGC TGCGGAGCTG CCCGCTGCAG 
GGCTCCCCCG GGCGCCCGCG CTCTGTCTGC GGCCGGGAAG GAGAGGAAAA ACCACCCTTA 
TCTGCAGAAA CACAATGGAA AGACAGAGCA GAAACAGTGA TAATTGGAGG TGGCTGTGTT 
GGTGTGAGTC TGGCTTATCA CCTGGCCAAA GCAGGGATGA AAGATGTGGT CCTGCTGGAG 
AAATCAGAGC TCACGGCTGG ATCTACCTGG CACGCAGCAG GTTTAACAAC TTACTTTCAT 
CCTGGAATAA ACTTGAAGAA AATACATTAT GATAGCATCA AACTTTATGA GAAACTGGAA 
GAAGAAACTG GTCAGGTGGT GGGATTCCAT CAGCCAGGTA GTATCAGACT TGCTACCACC 
CCTGTAAGGG TAGATGAATT TAAATATCAA ATGACTCGGA CTGGCTGGCA TGCAACAGAA 
CAGTATCTCA TTGAACCTGA AAAAATTCAA GAGATGTTCC CTTTACTCAA CATGAATAAG 
GTTTTAGCTG GATTGTATAA TCCTGGAGAT GGTCACATTG ATCCTTATTC TCTAACTATG 
GCACTGGCTG CTGGGGCTAG GAAATGTGGT GCCCTTTTAA AATATCCTGC ACCAGTAACT 
TCTCTGAAAG CCAGGTCAGA TGGAACATGG GACGTTGAAA CACCACAGGG GTCTATGAGA 
GCAAATAGAA TTGTGAATGC TGCAGGATTT TGGGCTCGTG AAGTAGGTAA AATGATTGGA 
CTAGAACATC CTCTCATTCC GGTTCAACAT CAATATGTTG TTACATCGAC TATATCTGAA 
GTGAAAGCTT TGAAACGAGA ACTGCCTGTG CTCCGTGACC TGGAAGGATC ATATTATCTC 
CGACAGGAAA GGGATGGGCT TTTGTTTGGT CCATATGAAA GTCAAGAGAA AATGAAAGTT 
CAGGACTCCT GGGTCACCAA TGGAGTTCCT CCAGGTTTTG GAAAGGAACT CTTTGAGTCT 
GATCTAGATC GAATCATGGA ACACATCAAA GCTGCCATGG AAATGGTTCC TGTCTTGAAA 
AAGGCTGACA TCATCAATGT TGTCAATGGT CCTATCACGT ATTCTCCTGA CATTCTGCCT 
ATGGTGGGGC CCCATCAGGG GGTCAGAAAC TACTGGGTGG CTATAGGCTT TGGATATGGC 
ATAATCCACG CTGGTGGGGT AGGGAAATAT CTCAGTGACT GGATCCTGCA TGGAGAACCT 
CCTTTTGATC TGATAGAATT GGATCCTAAT CGCTATGGCA AATGGACAAC AACCCAGTAC 
ACTGAGGCCA AAGCAAGAGA ATCATATGGA TTCAACAATA TTGTTGGTTA TCCTAAAGAA 
GAACGGTTTG CTGGGAGGCC GACTCAACGA GTCAGTGGGC TCTATCAAAG GCTGGAGTCT 
AAGTGTTCCA TGGGGTTCCA TGCTGGCTGG GAGCAGCCGC ACTGGTTCTA CAAACCAGGC 
CAGGACACTC AGTACAGGCC AAGTTTTCGC CGCACAAACT GGTTTGAGCC TGTGGGCTCG 
GAGTATAAAC AGGTTATGCA AAGAGTAGCG GTAACTGACC TATCACCATT TGGCAAGTTT 
AACATCAAAG GCCAAGATTC CATTAGACTA CTGGACCATC TCTTTGCAAA TGTCATTCCA 
AAGGTGGGTT TTACAAATAT AAGTCACATG TTAACACCCA AGGGTCGAGT GTATGCTGAG 
CTGACTGTTT CTCACCAATC TCCTGGGGAG TTTCTTTTAA TTACTGGCTC TGGATCAGAA 
CTTCATGATC TTAGATGGAT TGAAGAAGAA GCAGTCAAAG GTGGATATGA TGTTGAAATT 
AAAAACATAA CTGATGAGCT TGGAGTTCTT GGAGTTGCTG GGCCACAGGC AAGAAAGGTC 
CTTCAGAAAC TGACCTCTGA AGATCTTAGT GATGATGTTT TCAAGTTTCT TCAAACCAAG 
TCCTTAAAGG TTTCCAACAT TCCTGTCACT GCTATTAGGA TATCTTATAC TGGTGAGCTG 
GGTTGGGAGC TGTATCACAG AAGAGAAGAT TCTGTGGCGC TGTATGACGC TATCATGAAT 
GCAGGCCAGG AGGAGGGAAT CGACAATTTT GGAACCTATG CCATGAATGC CTTACGCCTG 
GAGAAAGCCT TCAGAGCCTG GGGGTTAGAG ATGAACTGTG ATACAAATCC TTTGGAAGCT 
GGACTGGAAT ATTTTGTGAA GTTAAATAAG CCAGCAGACT TCATAGGAAA GCAAGCACTG 
AAACAGATTA AAGCCAAGGG GCTGAAACGA AGACTGGTCT GCCTCACCTT GGCAACGGAT 
GATGTTGATC CAGAGGGAAA TGAAAGCATC TGGTACAATG GCAAGGTGGT TGGCAACACG 
ACATCTGGAA GCTATAGCTA CAGCATCCAG AAGAGTCTGG CTTTCGCATA TGTCCCTGTA 
CAACTAAGTG AAGTGGGACA GCAAGTGGAA GTTGAACTAT TAGGCAAAAA TTACCCAGCA 
GTCATCATAC AAGAACCTTT GGTATTGACC GAACCAACCA GAAACCGGCT TCAGAAAAAA 
GGTGGAAAGG ACAAAACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037523.2
CDS	55..2655
Translation	MLRPGAQLLRGLLLRSCPLQGSPGRPRSVCGREGEEKPPLSAETQWKDRAETVIIGGGCVGVSLAYHLAKAGMKDVVLLEKSELTAGSTWHAAGLTTYFHPGINLKKIHYDSIKLYEKLEEETGQVVGFHQPGSIRLATTPVRVDEFKYQMTRTGWHATEQYLIEPEKIQEMFPLLNMNKVLAGLYNPGDGHIDPYSLTMALAAGARKCGALLKYPAPVTSLKARSDGTWDVETPQGSMRANRIVNAAGFWAREVGKMIGLEHPLIPVQHQYVVTSTISEVKALKRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKVQDSWVTNGVPPGFGKELFESDLDRIMEHIKAAMEMVPVLKKADIINVVNGPITYSPDILPMVGPHQGVRNYWVAIGFGYGIIHAGGVGKYLSDWILHGEPPFDLIELDPNRYGKWTTTQYTEAKARESYGFNNIVGYPKEERFAGRPTQRVSGLYQRLESKCSMGFHAGWEQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVAVTDLSPFGKFNIKGQDSIRLLDHLFANVIPKVGFTNISHMLTPKGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEEAVKGGYDVEIKNITDELGVLGVAGPQARKVLQKLTSEDLSDDVFKFLQTKSLKVSNIPVTAIRISYTGELGWELYHRREDSVALYDAIMNAGQEEGIDNFGTYAMNALRLEKAFRAWGLEMNCDTNPLEAGLEYFVKLNKPADFIGKQALKQIKAKGLKRRLVCLTLATDDVDPEGNESIWYNGKVVGNTTSGSYSYSIQKSLAFAYVPVQLSEVGQQVEVELLGKNYPAVIIQEPLVLTEPTRNRLQKKGGKDKT

Target ORF information:

RefSeq Version	NM_013391.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dimethylglycine dehydrogenase (DMGDH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013391.3

ORF Insert Sequence:

ATGCTCCGTC CCGGCGCGCA GCTGCTGCGG GGCCTCCTGC TGCGGAGCTG CCCGCTGCAG 
GGCTCCCCCG GGCGCCCGCG CTCTGTCTGC GGCCGGGAAG GAGAGGAAAA ACCACCCTTA 
TCTGCAGAAA CACAATGGAA AGACAGAGCA GAAACAGTGA TAATTGGAGG TGGCTGTGTT 
GGTGTGAGTC TGGCTTATCA CCTGGCCAAA GCAGGGATGA AAGATGTGGT CCTGCTGGAG 
AAATCAGAGC TCACGGCTGG ATCTACCTGG CACGCAGCAG GTTTAACAAC TTACTTTCAT 
CCTGGAATAA ACTTGAAGAA AATACATTAT GATAGCATCA AACTTTATGA GAAACTGGAA 
GAAGAAACTG GTCAGGTGGT GGGATTCCAT CAGCCAGGTA GTATCAGACT TGCTACCACC 
CCTGTAAGGG TAGATGAATT TAAATATCAA ATGACTCGGA CTGGCTGGCA TGCAACAGAA 
CAGTATCTCA TTGAACCTGA AAAAATTCAA GAGATGTTCC CTTTACTCAA CATGAATAAG 
GTTTTAGCTG GATTGTATAA TCCTGGAGAT GGTCACATTG ATCCTTATTC TCTAACTATG 
GCACTGGCTG CTGGGGCTAG GAAATGTGGT GCCCTTTTAA AATATCCTGC ACCAGTAACT 
TCTCTGAAAG CCAGGTCAGA TGGAACATGG GACGTTGAAA CACCACAGGG GTCTATGAGA 
GCAAATAGAA TTGTGAATGC TGCAGGATTT TGGGCTCGTG AAGTAGGTAA AATGATTGGA 
CTAGAACATC CTCTCATTCC GGTTCAACAT CAATATGTTG TTACATCGAC TATATCTGAA 
GTGAAAGCTT TGAAACGAGA ACTGCCTGTG CTCCGTGACC TGGAAGGATC ATATTATCTC 
CGACAGGAAA GGGATGGGCT TTTGTTTGGT CCATATGAAA GTCAAGAGAA AATGAAAGTT 
CAGGACTCCT GGGTCACCAA TGGAGTTCCT CCAGGTTTTG GAAAGGAACT CTTTGAGTCT 
GATCTAGATC GAATCATGGA ACACATCAAA GCTGCCATGG AAATGGTTCC TGTCTTGAAA 
AAGGCTGACA TCATCAATGT TGTCAATGGT CCTATCACGT ATTCTCCTGA CATTCTGCCT 
ATGGTGGGGC CCCATCAGGG GGTCAGAAAC TACTGGGTGG CTATAGGCTT TGGATATGGC 
ATAATCCACG CTGGTGGGGT AGGGAAATAT CTCAGTGACT GGATCCTGCA TGGAGAACCT 
CCTTTTGATC TGATAGAATT GGATCCTAAT CGCTATGGCA AATGGACAAC AACCCAGTAC 
ACTGAGGCCA AAGCAAGAGA ATCATATGGA TTCAACAATA TTGTTGGTTA TCCTAAAGAA 
GAACGGTTTG CTGGGAGGCC GACTCAACGA GTCAGTGGGC TCTATCAAAG GCTGGAGTCT 
AAGTGTTCCA TGGGGTTCCA TGCTGGCTGG GAGCAGCCGC ACTGGTTCTA CAAACCAGGC 
CAGGACACTC AGTACAGGCC AAGTTTTCGC CGCACAAACT GGTTTGAGCC TGTGGGCTCG 
GAGTATAAAC AGGTTATGCA AAGAGTAGCG GTAACTGACC TATCACCATT TGGCAAGTTT 
AACATCAAAG GCCAAGATTC CATTAGACTA CTGGACCATC TCTTTGCAAA TGTCATTCCA 
AAGGTGGGTT TTACAAATAT AAGTCACATG TTAACACCCA AGGGTCGAGT GTATGCTGAG 
CTGACTGTTT CTCACCAATC TCCTGGGGAG TTTCTTTTAA TTACTGGCTC TGGATCAGAA 
CTTCATGATC TTAGATGGAT TGAAGAAGAA GCAGTCAAAG GTGGATATGA TGTTGAAATT 
AAAAACATAA CTGATGAGCT TGGAGTTCTT GGAGTTGCTG GGCCACAGGC AAGAAAGGTC 
CTTCAGAAAC TGACCTCTGA AGATCTTAGT GATGATGTTT TCAAGTTTCT TCAAACCAAG 
TCCTTAAAGG TTTCCAACAT TCCTGTCACT GCTATTAGGA TATCTTATAC TGGTGAGCTG 
GGTTGGGAGC TGTATCACAG AAGAGAAGAT TCTGTGGCGC TGTATGACGC TATCATGAAT 
GCAGGCCAGG AGGAGGGAAT CGACAATTTT GGAACCTATG CCATGAATGC CTTACGCCTG 
GAGAAAGCCT TCAGAGCCTG GGGGTTAGAG ATGAACTGTG ATACAAATCC TTTGGAAGCT 
GGACTGGAAT ATTTTGTGAA GTTAAATAAG CCAGCAGACT TCATAGGAAA GCAAGCACTG 
AAACAGATTA AAGCCAAGGG GCTGAAACGA AGACTGGTCT GCCTCACCTT GGCAACGGAT 
GATGTTGATC CAGAGGGAAA TGAAAGCATC TGGTACAATG GCAAGGTGGT TGGCAACACG 
ACATCTGGAA GCTATAGCTA CAGCATCCAG AAGAGTCTGG CTTTCGCATA TGTCCCTGTA 
CAACTAAGTG AAGTGGGACA GCAAGTGGAA GTTGAACTAT TAGGCAAAAA TTACCCAGCA 
GTCATCATAC AAGAACCTTT GGTATTGACC GAACCAACCA GAAACCGGCT TCAGAAAAAA 
GGTGGAAAGG ACAAAACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMGDH ( XM_006714597.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714660.1 Homo sapiens dimethylglycine dehydrogenase (DMGDH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu39555
Clone ID Related Accession (Same CDS sequence)	XM_006714597.2
Accession Version	XM_006714597.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dimethylglycine dehydrogenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714597.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGTC CCGGCGCGCA GCTGCTGCGG GGCCTCCTGC TGCGGAGCTG CCCGCTGCAG 
GGCTCCCCCG GGCGCCCGCG CTCTGTCTGC GGCCGGGAAG GAGAGGAAAA ACCACCCTTA 
TCTGCAGAAA CACAATGGAA AGACAGAGCA GAAACAGTGA TAATTGGAGG TGGCTGTGTT 
GGTGTGAGTC TGGCTTATCA CCTGGCCAAA GCAGGGATGA AAGATGTGGT CCTGCTGGAG 
AAATCAGAGC TCACGGCTGG ATCTACCTGG CACGCAGCAG GTTTAACAAC TTACTTTCAT 
CCTGGAATAA ACTTGAAGAA AATACATTAT GATAGCATCA AACTTTATGA GAAACTGGAA 
GAAGAAACTG GTCAGGTGGT GGGATTCCAT CAGCCAGGTA GTATCAGACT TGCTACCACC 
CCTGTAAGGG TAGATGAATT TAAATATCAA ATGACTCGGA CTGGCTGGCA TGCAACAGAA 
CAGTATCTCA TTGAACCTGA AAAAATTCAA GAGATGTTCC CTTTACTCAA CATGAATAAG 
GTTTTAGCTG GATTGTATAA TCCTGGAGAT GGTCACATTG ATCCTTATTC TCTAACTATG 
GCACTGGCTG CTGGGGCTAG GAAATGTGGT GCCCTTTTAA AATATCCTGC ACCAGTAACT 
TCTCTGAAAG CCAGGTCAGA TGGAACATGG GACGTTGAAA CACCACAGGG GTCTATGAGA 
GCAAATAGAA TTGTGAATGC TGCAGGATTT TGGGCTCGTG AAGTAGGTAA AATGATTGGA 
CTAGAACATC CTCTCATTCC GGTTCAACAT CAATATGTTG TTACATCGAC TATATCTGAA 
GTGAAAGCTT TGAAACGAGA ACTGCCTGTG CTCCGTGACC TGGAAGGATC ATATTATCTC 
CGACAGGAAA GGGATGGGCT TTTGTTTGGT CCATATGAAA GTCAAGAGAA AATGAAAGTT 
CAGGACTCCT GGGTCACCAA TGGAGTTCCT CCAGGTTTTG GAAAGGAACT CTTTGAGTCT 
GATCTAGATC GAATCATGGA ACACATCAAA GCTGCCATGG AAATGGTTCC TGTCTTGAAA 
AAGGCTGACA TCATCAATGT TGTCAATGGT CCTATCACGT ATTCTCCTGA CATTCTGCCT 
ATGGTGGGGC CCCATCAGGG GGTCAGAAAC TACTGGGTGG CTATAGGCTT TGGATATGGC 
ATAATCCACG CTGGTGGGGT AGGGAAATAT CTCAGTGACT GGATCCTGCA TGGAGAACCT 
CCTTTTGATC TGATAGAATT GGATCCTAAT CGCTATGGCA AATGGACAAC AACCCAGTAC 
ACTGAGGCCA AAGCAAGAGA ATCATATGGA TTCAACAATA TTGTTGGTTA TCCTAAAGAA 
GAACGGTTTG CTGGGAGGCC GACTCAACGA GTCAGTGGGC TCTATCAAAG GCTGGAGTCT 
AAGTGTTCCA TGGGGTTCCA TGCTGGCTGG GAGCAGCCGC ACTGGTTCTA CAAACCAGGC 
CAGGACACTC AGTACAGGCC AAGTTTTCGC CGCACAAACT GGTTTGAGCC TGTGGGCTCG 
GAGTATAAAC AGGTTATGCA AAGAGTAGCG GTAACTGACC TATCACCATT TGGCAAGTTT 
AACATCAAAG GCCAAGATTC CATTAGACTA CTGGACCATC TCTTTGCAAA TGTCATTCCA 
AAGGTGGGTT TTACAAATAT AAGTCACATG TTAACACCCA AGGGTCGAGT GTATGCTGAG 
CTGACTGTTT CTCACCAATC TCCTGGGGAG TTTCTTTTAA TTACTGGCTC TGGATCAGAA 
CTTCATGATC TTAGATGGAT TGAAGAAGAA GCAGTCAAAG GTGGATATGA TGTTGAAATT 
AAAAACATAA CTGATGAGCT TGGAGTTCTT GGAGTTGCTG GGCCACAGGC AAGAAAGGTC 
CTTCAGAAAC TGACCTCTGA AGATCTTAGT GATGATGTTT TCAAGTTTCT TCAAACCAAG 
TCCTTAAAGG TTTCCAACAT TCCTGTCACT GCTATTAGGA TATCTTATAC TGGTGAGCTG 
GGTTGGGAGC TGTATCACAG AAGAGAAGAT TCTGTGGCGC TGTATGACGC TATCATGAAT 
GCAGGCCAGG AGGAGGGAAT CGACAATTTT GGAACCTATG CCATGAATGC CTTACGCCTG 
GAGAAAGCCT TCAGAGCCTG GGGGTTAGAG ATGAACTGTG ATACAAATCC TTTGGAAGCT 
GGACTGGAAT ATTTTGTGAA GTTAAATAAG GATCAGAATA GCTGCTTTGC CCATTTTAAG 
GAGGAAAATG GCTGGGTGAG CAGATGGGCC ATCAGGCCTT ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714660.1
CDS	13..2337
Translation	MLRPGAQLLRGLLLRSCPLQGSPGRPRSVCGREGEEKPPLSAETQWKDRAETVIIGGGCVGVSLAYHLAKAGMKDVVLLEKSELTAGSTWHAAGLTTYFHPGINLKKIHYDSIKLYEKLEEETGQVVGFHQPGSIRLATTPVRVDEFKYQMTRTGWHATEQYLIEPEKIQEMFPLLNMNKVLAGLYNPGDGHIDPYSLTMALAAGARKCGALLKYPAPVTSLKARSDGTWDVETPQGSMRANRIVNAAGFWAREVGKMIGLEHPLIPVQHQYVVTSTISEVKALKRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKVQDSWVTNGVPPGFGKELFESDLDRIMEHIKAAMEMVPVLKKADIINVVNGPITYSPDILPMVGPHQGVRNYWVAIGFGYGIIHAGGVGKYLSDWILHGEPPFDLIELDPNRYGKWTTTQYTEAKARESYGFNNIVGYPKEERFAGRPTQRVSGLYQRLESKCSMGFHAGWEQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVAVTDLSPFGKFNIKGQDSIRLLDHLFANVIPKVGFTNISHMLTPKGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEEAVKGGYDVEIKNITDELGVLGVAGPQARKVLQKLTSEDLSDDVFKFLQTKSLKVSNIPVTAIRISYTGELGWELYHRREDSVALYDAIMNAGQEEGIDNFGTYAMNALRLEKAFRAWGLEMNCDTNPLEAGLEYFVKLNKDQNSCFAHFKEENGWVSRWAIRPY

Target ORF information:

RefSeq Version	XM_006714597.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dimethylglycine dehydrogenase (DMGDH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714597.2

ORF Insert Sequence:

ATGCTCCGTC CCGGCGCGCA GCTGCTGCGG GGCCTCCTGC TGCGGAGCTG CCCGCTGCAG 
GGCTCCCCCG GGCGCCCGCG CTCTGTCTGC GGCCGGGAAG GAGAGGAAAA ACCACCCTTA 
TCTGCAGAAA CACAATGGAA AGACAGAGCA GAAACAGTGA TAATTGGAGG TGGCTGTGTT 
GGTGTGAGTC TGGCTTATCA CCTGGCCAAA GCAGGGATGA AAGATGTGGT CCTGCTGGAG 
AAATCAGAGC TCACGGCTGG ATCTACCTGG CACGCAGCAG GTTTAACAAC TTACTTTCAT 
CCTGGAATAA ACTTGAAGAA AATACATTAT GATAGCATCA AACTTTATGA GAAACTGGAA 
GAAGAAACTG GTCAGGTGGT GGGATTCCAT CAGCCAGGTA GTATCAGACT TGCTACCACC 
CCTGTAAGGG TAGATGAATT TAAATATCAA ATGACTCGGA CTGGCTGGCA TGCAACAGAA 
CAGTATCTCA TTGAACCTGA AAAAATTCAA GAGATGTTCC CTTTACTCAA CATGAATAAG 
GTTTTAGCTG GATTGTATAA TCCTGGAGAT GGTCACATTG ATCCTTATTC TCTAACTATG 
GCACTGGCTG CTGGGGCTAG GAAATGTGGT GCCCTTTTAA AATATCCTGC ACCAGTAACT 
TCTCTGAAAG CCAGGTCAGA TGGAACATGG GACGTTGAAA CACCACAGGG GTCTATGAGA 
GCAAATAGAA TTGTGAATGC TGCAGGATTT TGGGCTCGTG AAGTAGGTAA AATGATTGGA 
CTAGAACATC CTCTCATTCC GGTTCAACAT CAATATGTTG TTACATCGAC TATATCTGAA 
GTGAAAGCTT TGAAACGAGA ACTGCCTGTG CTCCGTGACC TGGAAGGATC ATATTATCTC 
CGACAGGAAA GGGATGGGCT TTTGTTTGGT CCATATGAAA GTCAAGAGAA AATGAAAGTT 
CAGGACTCCT GGGTCACCAA TGGAGTTCCT CCAGGTTTTG GAAAGGAACT CTTTGAGTCT 
GATCTAGATC GAATCATGGA ACACATCAAA GCTGCCATGG AAATGGTTCC TGTCTTGAAA 
AAGGCTGACA TCATCAATGT TGTCAATGGT CCTATCACGT ATTCTCCTGA CATTCTGCCT 
ATGGTGGGGC CCCATCAGGG GGTCAGAAAC TACTGGGTGG CTATAGGCTT TGGATATGGC 
ATAATCCACG CTGGTGGGGT AGGGAAATAT CTCAGTGACT GGATCCTGCA TGGAGAACCT 
CCTTTTGATC TGATAGAATT GGATCCTAAT CGCTATGGCA AATGGACAAC AACCCAGTAC 
ACTGAGGCCA AAGCAAGAGA ATCATATGGA TTCAACAATA TTGTTGGTTA TCCTAAAGAA 
GAACGGTTTG CTGGGAGGCC GACTCAACGA GTCAGTGGGC TCTATCAAAG GCTGGAGTCT 
AAGTGTTCCA TGGGGTTCCA TGCTGGCTGG GAGCAGCCGC ACTGGTTCTA CAAACCAGGC 
CAGGACACTC AGTACAGGCC AAGTTTTCGC CGCACAAACT GGTTTGAGCC TGTGGGCTCG 
GAGTATAAAC AGGTTATGCA AAGAGTAGCG GTAACTGACC TATCACCATT TGGCAAGTTT 
AACATCAAAG GCCAAGATTC CATTAGACTA CTGGACCATC TCTTTGCAAA TGTCATTCCA 
AAGGTGGGTT TTACAAATAT AAGTCACATG TTAACACCCA AGGGTCGAGT GTATGCTGAG 
CTGACTGTTT CTCACCAATC TCCTGGGGAG TTTCTTTTAA TTACTGGCTC TGGATCAGAA 
CTTCATGATC TTAGATGGAT TGAAGAAGAA GCAGTCAAAG GTGGATATGA TGTTGAAATT 
AAAAACATAA CTGATGAGCT TGGAGTTCTT GGAGTTGCTG GGCCACAGGC AAGAAAGGTC 
CTTCAGAAAC TGACCTCTGA AGATCTTAGT GATGATGTTT TCAAGTTTCT TCAAACCAAG 
TCCTTAAAGG TTTCCAACAT TCCTGTCACT GCTATTAGGA TATCTTATAC TGGTGAGCTG 
GGTTGGGAGC TGTATCACAG AAGAGAAGAT TCTGTGGCGC TGTATGACGC TATCATGAAT 
GCAGGCCAGG AGGAGGGAAT CGACAATTTT GGAACCTATG CCATGAATGC CTTACGCCTG 
GAGAAAGCCT TCAGAGCCTG GGGGTTAGAG ATGAACTGTG ATACAAATCC TTTGGAAGCT 
GGACTGGAAT ATTTTGTGAA GTTAAATAAG GATCAGAATA GCTGCTTTGC CCATTTTAAG 
GAGGAAAATG GCTGGGTGAG CAGATGGGCC ATCAGGCCTT ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMGDH ( XM_011543354.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541656.1 Homo sapiens dimethylglycine dehydrogenase (DMGDH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu67433
Clone ID Related Accession (Same CDS sequence)	XM_011543354.2
Accession Version	XM_011543354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	dimethylglycine dehydrogenase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGTC CCGGCGCGCA GCTGCTGCGG GGCCTCCTGC TGCGGAGCTG CCCGCTGCAG 
GGCTCCCCCG GGCGCCCGCG CTCTGTCTGC GGCCGGGAAG GAGAGGAAAA ACCACCCTTA 
TCTGCAGAAA CACAATGGAA AGACAGAGCA GAAACAGTGA TAATTGGAGG TGGCTGTGTT 
GGTGTGAGTC TGGCTTATCA CCTGGCCAAA GCAGGGATGA AAGATGTGGT CCTGCTGGAG 
AAATCAGAGC TCACGGCTGG ATCTACCTGG CACGCAGCAG GTTTAACAAC TTACTTTCAT 
CCTGGAATAA ACTTGAAGAA AATACATTAT GATAGCATCA AACTTTATGA GAAACTGGAA 
GAAGAAACTG GTCAGGTGGT GGGATTCCAT CAGCCAGGTA GTATCAGACT TGCTACCACC 
CCTGTAAGGG TAGATGAATT TAAATATCAA ATGACTCGGA CTGGCTGGCA TGCAACAGAA 
CAGTATCTCA TTGAACCTGA AAAAATTCAA GAGATGTTCC CTTTACTCAA CATGAATAAG 
GTTTTAGCTG GATTGTATAA TCCTGGAGAT GGTCACATTG ATCCTTATTC TCTAACTATG 
GCACTGGCTG CTGGGGCTAG GAAATGTGGT GCCCTTTTAA AATATCCTGC ACCAGTAACT 
TCTCTGAAAG CCAGGTCAGA TGGAACATGG GACGTTGAAA CACCACAGGG GTCTATGAGA 
GCAAATAGAA TTGTGAATGC TGCAGGATTT TGGGCTCGTG AAGTAGGTAA AATGATTGGA 
CTAGAACATC CTCTCATTCC GGTTCAACAT CAATATGTTG TTACATCGAC TATATCTGAA 
GTGAAAGCTT TGAAACGAGA ACTGCCTGTG CTCCGTGACC TGGAAGGATC ATATTATCTC 
CGACAGGAAA GGGATGGGCT TTTGTTTGGT CCATATGAAA GTCAAGAGAA AATGAAAGTT 
CAGGACTCCT GGGTCACCAA TGGAGTTCCT CCAGGTTTTG GAAAGGAACT CTTTGAGTCT 
GATCTAGATC GAATCATGGA ACACATCAAA GCTGCCATGG AAATGGTTCC TGTCTTGAAA 
AAGGCTGACA TCATCAATGT TGTCAATGGT CCTATCACGT ATTCTCCTGA CATTCTGCCT 
ATGGTGGGGC CCCATCAGGG GGTCAGAAAC TACTGGGTGG CTATAGGCTT TGGATATGGC 
ATAATCCACG CTGGTGGGGT AGGGAAATAT CTCAGTGACT GGATCCTGCA TGGAGAACCT 
CCTTTTGATC TGATAGAATT GGATCCTAAT CGCTATGGCA AATGGACAAC AACCCAGTAC 
ACTGAGGCCA AAGCAAGAGA ATCATATGGA TTCAACAATA TTGTTGGTTA TCCTAAAGAA 
GAACGGTTTG CTGGGAGGCC GACTCAACGA GTCAGTGGGC TCTATCAAAG GCTGGAGTCT 
AAGTGTTCCA TGGGGTTCCA TGCTGGCTGG GAGCAGCCGC ACTGGTTCTA CAAACCAGGC 
CAGGACACTC AGTACAGGCC AAGTTTTCGC CGCACAAACT GGTTTGAGCC TGTGGGCTCG 
GAGTATAAAC AGGTTATGCA AAGAGTAGCG GTAACTGACC TATCACCATT TGGCAAGTTT 
AACATCAAAG GCCAAGATTC CATTAGACTA CTGGACCATC TCTTTGCAAA TGTCATTCCA 
AAGGTGGGTT TTACAAATAT AAGTCACATG TTAACACCCA AGGGTCGAGT GTATGCTGAG 
CTGACTGTTT CTCACCAATC TCCTGGGGAG TTTCTTTTAA TTACTGGCTC TGGATCAGAA 
CTTCATGATC TTAGATGGAT TGAAGAAGAA GCAGTCAAAG GTGGATATGA TGTTGAAATT 
AAAAACATAA CTGATGAGCT TGGAGTTCTT GGAGTTGCTG GGCCACAGGC AAGAAAGGTC 
CTTCAGAAAC TGACCTCTGA AGATCTTAGT GATGATGTTT TCAAGTTTCT TCAAACCAAG 
TCCTTAAAGG TTTCCAACAT TCCTGTCACT GCTATTAGGA TATCTTATAC TGGTGAGCTG 
GGTTGGGAGC TGTATCACAG AAGAGAAGAT TCTGTGGCGC TGTATGACGC TATCATGAAT 
GCAGGCCAGG AGGAGGGAAT CGACAATTTT GGAACCTATG CCATGAATGC CTTACGCCTG 
GAGAAAGCCT TCAGAGCCTG GGGGTTAGAG ATGAACTGTG ATACAAATCC TTTGGAAGCT 
GGACTGGAAT ATTTTGTGAA GTTAAATAAG GGATCTATGG ATCTTAGAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541656.1
CDS	13..2286
Translation	MLRPGAQLLRGLLLRSCPLQGSPGRPRSVCGREGEEKPPLSAETQWKDRAETVIIGGGCVGVSLAYHLAKAGMKDVVLLEKSELTAGSTWHAAGLTTYFHPGINLKKIHYDSIKLYEKLEEETGQVVGFHQPGSIRLATTPVRVDEFKYQMTRTGWHATEQYLIEPEKIQEMFPLLNMNKVLAGLYNPGDGHIDPYSLTMALAAGARKCGALLKYPAPVTSLKARSDGTWDVETPQGSMRANRIVNAAGFWAREVGKMIGLEHPLIPVQHQYVVTSTISEVKALKRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKVQDSWVTNGVPPGFGKELFESDLDRIMEHIKAAMEMVPVLKKADIINVVNGPITYSPDILPMVGPHQGVRNYWVAIGFGYGIIHAGGVGKYLSDWILHGEPPFDLIELDPNRYGKWTTTQYTEAKARESYGFNNIVGYPKEERFAGRPTQRVSGLYQRLESKCSMGFHAGWEQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVAVTDLSPFGKFNIKGQDSIRLLDHLFANVIPKVGFTNISHMLTPKGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEEAVKGGYDVEIKNITDELGVLGVAGPQARKVLQKLTSEDLSDDVFKFLQTKSLKVSNIPVTAIRISYTGELGWELYHRREDSVALYDAIMNAGQEEGIDNFGTYAMNALRLEKAFRAWGLEMNCDTNPLEAGLEYFVKLNKGSMDLRN

Target ORF information:

RefSeq Version	XM_011543354.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dimethylglycine dehydrogenase (DMGDH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543354.2

ORF Insert Sequence:

ATGCTCCGTC CCGGCGCGCA GCTGCTGCGG GGCCTCCTGC TGCGGAGCTG CCCGCTGCAG 
GGCTCCCCCG GGCGCCCGCG CTCTGTCTGC GGCCGGGAAG GAGAGGAAAA ACCACCCTTA 
TCTGCAGAAA CACAATGGAA AGACAGAGCA GAAACAGTGA TAATTGGAGG TGGCTGTGTT 
GGTGTGAGTC TGGCTTATCA CCTGGCCAAA GCAGGGATGA AAGATGTGGT CCTGCTGGAG 
AAATCAGAGC TCACGGCTGG ATCTACCTGG CACGCAGCAG GTTTAACAAC TTACTTTCAT 
CCTGGAATAA ACTTGAAGAA AATACATTAT GATAGCATCA AACTTTATGA GAAACTGGAA 
GAAGAAACTG GTCAGGTGGT GGGATTCCAT CAGCCAGGTA GTATCAGACT TGCTACCACC 
CCTGTAAGGG TAGATGAATT TAAATATCAA ATGACTCGGA CTGGCTGGCA TGCAACAGAA 
CAGTATCTCA TTGAACCTGA AAAAATTCAA GAGATGTTCC CTTTACTCAA CATGAATAAG 
GTTTTAGCTG GATTGTATAA TCCTGGAGAT GGTCACATTG ATCCTTATTC TCTAACTATG 
GCACTGGCTG CTGGGGCTAG GAAATGTGGT GCCCTTTTAA AATATCCTGC ACCAGTAACT 
TCTCTGAAAG CCAGGTCAGA TGGAACATGG GACGTTGAAA CACCACAGGG GTCTATGAGA 
GCAAATAGAA TTGTGAATGC TGCAGGATTT TGGGCTCGTG AAGTAGGTAA AATGATTGGA 
CTAGAACATC CTCTCATTCC GGTTCAACAT CAATATGTTG TTACATCGAC TATATCTGAA 
GTGAAAGCTT TGAAACGAGA ACTGCCTGTG CTCCGTGACC TGGAAGGATC ATATTATCTC 
CGACAGGAAA GGGATGGGCT TTTGTTTGGT CCATATGAAA GTCAAGAGAA AATGAAAGTT 
CAGGACTCCT GGGTCACCAA TGGAGTTCCT CCAGGTTTTG GAAAGGAACT CTTTGAGTCT 
GATCTAGATC GAATCATGGA ACACATCAAA GCTGCCATGG AAATGGTTCC TGTCTTGAAA 
AAGGCTGACA TCATCAATGT TGTCAATGGT CCTATCACGT ATTCTCCTGA CATTCTGCCT 
ATGGTGGGGC CCCATCAGGG GGTCAGAAAC TACTGGGTGG CTATAGGCTT TGGATATGGC 
ATAATCCACG CTGGTGGGGT AGGGAAATAT CTCAGTGACT GGATCCTGCA TGGAGAACCT 
CCTTTTGATC TGATAGAATT GGATCCTAAT CGCTATGGCA AATGGACAAC AACCCAGTAC 
ACTGAGGCCA AAGCAAGAGA ATCATATGGA TTCAACAATA TTGTTGGTTA TCCTAAAGAA 
GAACGGTTTG CTGGGAGGCC GACTCAACGA GTCAGTGGGC TCTATCAAAG GCTGGAGTCT 
AAGTGTTCCA TGGGGTTCCA TGCTGGCTGG GAGCAGCCGC ACTGGTTCTA CAAACCAGGC 
CAGGACACTC AGTACAGGCC AAGTTTTCGC CGCACAAACT GGTTTGAGCC TGTGGGCTCG 
GAGTATAAAC AGGTTATGCA AAGAGTAGCG GTAACTGACC TATCACCATT TGGCAAGTTT 
AACATCAAAG GCCAAGATTC CATTAGACTA CTGGACCATC TCTTTGCAAA TGTCATTCCA 
AAGGTGGGTT TTACAAATAT AAGTCACATG TTAACACCCA AGGGTCGAGT GTATGCTGAG 
CTGACTGTTT CTCACCAATC TCCTGGGGAG TTTCTTTTAA TTACTGGCTC TGGATCAGAA 
CTTCATGATC TTAGATGGAT TGAAGAAGAA GCAGTCAAAG GTGGATATGA TGTTGAAATT 
AAAAACATAA CTGATGAGCT TGGAGTTCTT GGAGTTGCTG GGCCACAGGC AAGAAAGGTC 
CTTCAGAAAC TGACCTCTGA AGATCTTAGT GATGATGTTT TCAAGTTTCT TCAAACCAAG 
TCCTTAAAGG TTTCCAACAT TCCTGTCACT GCTATTAGGA TATCTTATAC TGGTGAGCTG 
GGTTGGGAGC TGTATCACAG AAGAGAAGAT TCTGTGGCGC TGTATGACGC TATCATGAAT 
GCAGGCCAGG AGGAGGGAAT CGACAATTTT GGAACCTATG CCATGAATGC CTTACGCCTG 
GAGAAAGCCT TCAGAGCCTG GGGGTTAGAG ATGAACTGTG ATACAAATCC TTTGGAAGCT 
GGACTGGAAT ATTTTGTGAA GTTAAATAAG GGATCTATGG ATCTTAGAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMGDH ( XM_011543355.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541657.1 Homo sapiens dimethylglycine dehydrogenase (DMGDH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu67434
Clone ID Related Accession (Same CDS sequence)	XM_011543355.2
Accession Version	XM_011543355.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dimethylglycine dehydrogenase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGTC CCGGCGCGCA GCTGCTGCGG GGCCTCCTGC TGCGGAGCTG CCCGCTGCAG 
GGCTCCCCCG GGCGCCCGCG CTCTGTCTGC GGCCGGGAAG GAGAGGAAAA ACCACCCTTA 
TCTGCAGAAA CACAATGGAA AGACAGAGCA GAAACAGTGA TAATTGGAGG TGGCTGTGTT 
GGTGTGAGTC TGGCTTATCA CCTGGCCAAA GCAGGGATGA AAGATGTGGT CCTGCTGGAG 
AAATCAGAGC TCACGGCTGG ATCTACCTGG CACGCAGCAG GTTTAACAAC TTACTTTCAT 
CCTGGAATAA ACTTGAAGAA AATACATTAT GATAGCATCA AACTTTATGA GAAACTGGAA 
GAAGAAACTG GTCAGGTGGT GGGATTCCAT CAGCCAGGTA GTATCAGACT TGCTACCACC 
CCTGTAAGGG TAGATGAATT TAAATATCAA ATGACTCGGA CTGGCTGGCA TGCAACAGAA 
CAGTATCTCA TTGAACCTGA AAAAATTCAA GAGATGTTCC CTTTACTCAA CATGAATAAG 
GTTTTAGCTG GATTGTATAA TCCTGGAGAT GGTCACATTG ATCCTTATTC TCTAACTATG 
GCACTGGCTG CTGGGGCTAG GAAATGTGGT GCCCTTTTAA AATATCCTGC ACCAGTAACT 
TCTCTGAAAG CCAGGTCAGA TGGAACATGG GACGTTGAAA CACCACAGGG GTCTATGAGA 
GCAAATAGAA TTGTGAATGC TGCAGGATTT TGGGCTCGTG AAGTAGGTAA AATGATTGGA 
CTAGAACATC CTCTCATTCC GGTTCAACAT CAATATGTTG TTACATCGAC TATATCTGAA 
GTGAAAGCTT TGAAACGAGA ACTGCCTGTG CTCCGTGACC TGGAAGGATC ATATTATCTC 
CGACAGGAAA GGGATGGGCT TTTGTTTGGT CCATATGAAA GTCAAGAGAA AATGAAAGTT 
CAGGACTCCT GGGTCACCAA TGGAGTTCCT CCAGGTTTTG GAAAGGAACT CTTTGAGTCT 
GATCTAGATC GAATCATGGA ACACATCAAA GCTGCCATGG AAATGGTTCC TGTCTTGAAA 
AAGGCTGACA TCATCAATGT TGTCAATGGT CCTATCACGT ATTCTCCTGA CATTCTGCCT 
ATGGTGGGGC CCCATCAGGG GGTCAGAAAC TACTGGGTGG CTATAGGCTT TGGATATGGC 
ATAATCCACG CTGGTGGGGT AGGGAAATAT CTCAGTGACT GGATCCTGCA TGGAGAACCT 
CCTTTTGATC TGATAGAATT GGATCCTAAT CGCTATGGCA AATGGACAAC AACCCAGTAC 
ACTGAGGCCA AAGCAAGAGA ATCATATGGA TTCAACAATA TTGTTGGTTA TCCTAAAGAA 
GAACGGTTTG CTGGGAGGCC GACTCAACGA GTCAGTGGGC TCTATCAAAG GCTGGAGTCT 
AAGTGTTCCA TGGGGTTCCA TGCTGGCTGG GAGCAGCCGC ACTGGTTCTA CAAACCAGGC 
CAGGACACTC AGTACAGGCC AAGTTTTCGC CGCACAAACT GGTTTGAGCC TGTGGGCTCG 
GAGTATAAAC AGGTTATGCA AAGAGTAGCG GTAACTGACC TATCACCATT TGGCAAGTTT 
AACATCAAAG GCCAAGATTC CATTAGACTA CTGGACCATC TCTTTGCAAA TGTCATTCCA 
AAGGTGGGTT TTACAAATAT AAGTCACATG TTAACACCCA AGGGTCGAGT GTATGCTGAG 
CTGACTGTTT CTCACCAATC TCCTGGGGAG TTTCTTTTAA TTACTGGCTC TGGATCAGAA 
CTTCATGATC TTAGATGGAT TGAAGAAGAA GCAGTCAAAG GTGGATATGA TGTTGAAATT 
AAAAACATAA CTGATGAGCT TGGAGTTCTT GGAGTTGCTG GGCCACAGGC AAGAAAGGTC 
CTTCAGAAAC TGACCTCTGA AGATCTTAGT GATGATGTTT TCAAGTTTCT TCAAACCAAG 
TCCTTAAAGG TTTCCAACAT TCCTGTCACT GCTATTAGGA TATCTTATAC TGGTGAGCTG 
GGTTGGGAGC TGTATCACAG AAGAGAAGAT TCTGTGGCGC TGTATGACGC TATCATGAAT 
GCAGGCCAGG AGGAGGGAAT CGACAATTTT GGAACCTATG CCATGAATGC CTTACGCCTG 
GAGAAAGCCT TCAGAGCCTG GGGGTTAGAG ATGAACTGTG ATACAAATCC TTTGGAAGCT 
GGACTGGAAT ATTTTGTGAA GTTAAATAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541657.1
CDS	13..2265
Translation	MLRPGAQLLRGLLLRSCPLQGSPGRPRSVCGREGEEKPPLSAETQWKDRAETVIIGGGCVGVSLAYHLAKAGMKDVVLLEKSELTAGSTWHAAGLTTYFHPGINLKKIHYDSIKLYEKLEEETGQVVGFHQPGSIRLATTPVRVDEFKYQMTRTGWHATEQYLIEPEKIQEMFPLLNMNKVLAGLYNPGDGHIDPYSLTMALAAGARKCGALLKYPAPVTSLKARSDGTWDVETPQGSMRANRIVNAAGFWAREVGKMIGLEHPLIPVQHQYVVTSTISEVKALKRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKVQDSWVTNGVPPGFGKELFESDLDRIMEHIKAAMEMVPVLKKADIINVVNGPITYSPDILPMVGPHQGVRNYWVAIGFGYGIIHAGGVGKYLSDWILHGEPPFDLIELDPNRYGKWTTTQYTEAKARESYGFNNIVGYPKEERFAGRPTQRVSGLYQRLESKCSMGFHAGWEQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVAVTDLSPFGKFNIKGQDSIRLLDHLFANVIPKVGFTNISHMLTPKGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEEAVKGGYDVEIKNITDELGVLGVAGPQARKVLQKLTSEDLSDDVFKFLQTKSLKVSNIPVTAIRISYTGELGWELYHRREDSVALYDAIMNAGQEEGIDNFGTYAMNALRLEKAFRAWGLEMNCDTNPLEAGLEYFVKLNK

Target ORF information:

RefSeq Version	XM_011543355.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dimethylglycine dehydrogenase (DMGDH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543355.2

ORF Insert Sequence:

ATGCTCCGTC CCGGCGCGCA GCTGCTGCGG GGCCTCCTGC TGCGGAGCTG CCCGCTGCAG 
GGCTCCCCCG GGCGCCCGCG CTCTGTCTGC GGCCGGGAAG GAGAGGAAAA ACCACCCTTA 
TCTGCAGAAA CACAATGGAA AGACAGAGCA GAAACAGTGA TAATTGGAGG TGGCTGTGTT 
GGTGTGAGTC TGGCTTATCA CCTGGCCAAA GCAGGGATGA AAGATGTGGT CCTGCTGGAG 
AAATCAGAGC TCACGGCTGG ATCTACCTGG CACGCAGCAG GTTTAACAAC TTACTTTCAT 
CCTGGAATAA ACTTGAAGAA AATACATTAT GATAGCATCA AACTTTATGA GAAACTGGAA 
GAAGAAACTG GTCAGGTGGT GGGATTCCAT CAGCCAGGTA GTATCAGACT TGCTACCACC 
CCTGTAAGGG TAGATGAATT TAAATATCAA ATGACTCGGA CTGGCTGGCA TGCAACAGAA 
CAGTATCTCA TTGAACCTGA AAAAATTCAA GAGATGTTCC CTTTACTCAA CATGAATAAG 
GTTTTAGCTG GATTGTATAA TCCTGGAGAT GGTCACATTG ATCCTTATTC TCTAACTATG 
GCACTGGCTG CTGGGGCTAG GAAATGTGGT GCCCTTTTAA AATATCCTGC ACCAGTAACT 
TCTCTGAAAG CCAGGTCAGA TGGAACATGG GACGTTGAAA CACCACAGGG GTCTATGAGA 
GCAAATAGAA TTGTGAATGC TGCAGGATTT TGGGCTCGTG AAGTAGGTAA AATGATTGGA 
CTAGAACATC CTCTCATTCC GGTTCAACAT CAATATGTTG TTACATCGAC TATATCTGAA 
GTGAAAGCTT TGAAACGAGA ACTGCCTGTG CTCCGTGACC TGGAAGGATC ATATTATCTC 
CGACAGGAAA GGGATGGGCT TTTGTTTGGT CCATATGAAA GTCAAGAGAA AATGAAAGTT 
CAGGACTCCT GGGTCACCAA TGGAGTTCCT CCAGGTTTTG GAAAGGAACT CTTTGAGTCT 
GATCTAGATC GAATCATGGA ACACATCAAA GCTGCCATGG AAATGGTTCC TGTCTTGAAA 
AAGGCTGACA TCATCAATGT TGTCAATGGT CCTATCACGT ATTCTCCTGA CATTCTGCCT 
ATGGTGGGGC CCCATCAGGG GGTCAGAAAC TACTGGGTGG CTATAGGCTT TGGATATGGC 
ATAATCCACG CTGGTGGGGT AGGGAAATAT CTCAGTGACT GGATCCTGCA TGGAGAACCT 
CCTTTTGATC TGATAGAATT GGATCCTAAT CGCTATGGCA AATGGACAAC AACCCAGTAC 
ACTGAGGCCA AAGCAAGAGA ATCATATGGA TTCAACAATA TTGTTGGTTA TCCTAAAGAA 
GAACGGTTTG CTGGGAGGCC GACTCAACGA GTCAGTGGGC TCTATCAAAG GCTGGAGTCT 
AAGTGTTCCA TGGGGTTCCA TGCTGGCTGG GAGCAGCCGC ACTGGTTCTA CAAACCAGGC 
CAGGACACTC AGTACAGGCC AAGTTTTCGC CGCACAAACT GGTTTGAGCC TGTGGGCTCG 
GAGTATAAAC AGGTTATGCA AAGAGTAGCG GTAACTGACC TATCACCATT TGGCAAGTTT 
AACATCAAAG GCCAAGATTC CATTAGACTA CTGGACCATC TCTTTGCAAA TGTCATTCCA 
AAGGTGGGTT TTACAAATAT AAGTCACATG TTAACACCCA AGGGTCGAGT GTATGCTGAG 
CTGACTGTTT CTCACCAATC TCCTGGGGAG TTTCTTTTAA TTACTGGCTC TGGATCAGAA 
CTTCATGATC TTAGATGGAT TGAAGAAGAA GCAGTCAAAG GTGGATATGA TGTTGAAATT 
AAAAACATAA CTGATGAGCT TGGAGTTCTT GGAGTTGCTG GGCCACAGGC AAGAAAGGTC 
CTTCAGAAAC TGACCTCTGA AGATCTTAGT GATGATGTTT TCAAGTTTCT TCAAACCAAG 
TCCTTAAAGG TTTCCAACAT TCCTGTCACT GCTATTAGGA TATCTTATAC TGGTGAGCTG 
GGTTGGGAGC TGTATCACAG AAGAGAAGAT TCTGTGGCGC TGTATGACGC TATCATGAAT 
GCAGGCCAGG AGGAGGGAAT CGACAATTTT GGAACCTATG CCATGAATGC CTTACGCCTG 
GAGAAAGCCT TCAGAGCCTG GGGGTTAGAG ATGAACTGTG ATACAAATCC TTTGGAAGCT 
GGACTGGAAT ATTTTGTGAA GTTAAATAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evidence of interaction between genes in the folate/homocysteine metabolic pathway in controlling risk of nonsyndromic oral cleft.
Oral diseases(2018 Jan)
Wang P,Wu T,Schwender H,Wang H,Shi B,Wang Z,Yuan Y,Liu D,Wang M,Li J,Zhou Z,Zhu H,Beaty TH

Nonalcoholic steatohepatitis is associated with a state of betaine-insufficiency.
Liver international : official journal of the International Association for the Study of the Liver37(4)611-619(2017 Apr)
Sookoian S,Puri P,Casta?o GO,Scian R,Mirshahi F,Sanyal AJ,Pirola CJ

Structure and biochemical properties of recombinant human dimethylglycine dehydrogenase and comparison to the disease-related H109R variant.
The FEBS journal283(19)3587-3603(2016 10)
Augustin P,Hromic A,Pavkov-Keller T,Gruber K,Macheroux P

Genetic polymorphisms that affect selenium status and response to selenium supplementation in United Kingdom pregnant women.
The American journal of clinical nutrition103(1)100-6(2016 Jan)
Mao J,Vanderlelie JJ,Perkins AV,Redman CW,Ahmadi KR,Rayman MP

Dimethylglycine Deficiency and the Development of Diabetes.
Diabetes64(8)3010-6(2015 Aug)
Magnusson M,Wang TJ,Clish C,Engstr?m G,Nilsson P,Gerszten RE,Melander O

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DMGDH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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