TIMM22 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIMM22
Entrez Gene ID	29928
Full Name	translocase of inner mitochondrial membrane 22
Synonyms	TEX4,TIM22
General protein information	Preferred Names translocase of inner mitochondrial membrane 22 Names mitochondrial import inner membrane translocase subunit Tim22 testis-expressed protein 4 testis-expressed sequence 4 translocase of inner mitochondrial membrane 22 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.3
Summary	Multipass transmembrane proteins are brought into mitochondria and inserted into the mitochondrial inner membrane by way of the TIM22 complex. This complex has six subunits and is a twin-pore translocase. The protein encoded by this gene is a subunit of TIM22 and represents the voltage-activated and signal-gated channel. [provided by RefSeq, Jul 2016].
Disorder MIM:	607251

mRNA and Protein(s)

mRNA	Protein	Name
NM_013337.4	NP_037469.2	mitochondrial import inner membrane translocase subunit Tim22
NM_013337.2	NP_037469.2	mitochondrial import inner membrane translocase subunit Tim22
NM_013337.3	NP_037469.2	mitochondrial import inner membrane translocase subunit Tim22

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Timm22	NP_116007.1
Gallus gallus (chicken)	TIMM22	XP_415839.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003199	XP_312898.3
Xenopus tropicalis (tropical clawed frog)	timm22	NP_001011397.1
Homo sapiens (human)	TIMM22	NP_037469.2
Canis lupus familiaris (dog)	TIMM22	XP_537758.1
Mus musculus (house mouse)	Timm22	NP_062792.2
Bos taurus (cattle)	TIMM22	NP_001029670.1
Drosophila melanogaster (fruit fly)	CG31229	NP_732364.1
Caenorhabditis elegans (roundworm)	CELE_C47G2.3	NP_496412.1
Pan troglodytes (chimpanzee)	TIMM22	XP_001153337.1
Danio rerio (zebrafish)	LOC100537779	XP_003198763.1
Schizosaccharomyces pombe (fission yeast)	tim22	NP_596362.1

Gene Ontology
Bibliography

Related articles in PubMed

Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.
Kang Y, Stroud DA, Baker MJ, De Souza DP, Frazier AE, Liem M, Tull D, Mathivanan S, McConville MJ, Thorburn DR, Ryan MT, Stojanovski D
Molecular cell67(3)457-470.e5(2017 Aug)

Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.
Vukotic M, Nolte H, K?nig T, Saita S, Ananjew M, Kr?ger M, Tatsuta T, Langer T
Molecular cell67(3)471-483.e7(2017 Aug)

TIM29 is a subunit of the human carrier translocase required for protein transport.
Callegari S, Richter F, Chojnacka K, Jans DC, Lorenzi I, Pacheu-Grau D, Jakobs S, Lenz C, Urlaub H, Dudek J, Chacinska A, Rehling P
FEBS letters590(23)4147-4158(2016 Dec)

Organization and function of the small Tim complexes acting along the import pathway of metabolite carriers into mammalian mitochondria.
M?hlenbein N, Hofmann S, Rothbauer U, Bauer MF
The Journal of biological chemistry279(14)13540-6(2004 Apr)

Tim22, the essential core of the mitochondrial protein insertion complex, forms a voltage-activated and signal-gated channel.
Kovermann P, Truscott KN, Guiard B, Rehling P, Sepuri NB, M?ller H, Jensen RE, Wagner R, Pfanner N
Molecular cell9(2)363-73(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Identify AGK as a bona fide subunit of TIM22 providing an exciting and unexpected link between mitochondrial protein import and Sengers syndrome.
Title: Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.

The dual function of AGK as lipid kinase and constituent of the TIM22 complex reveals that disturbances in both phospholipid metabolism and mitochondrial protein biogenesis contribute to the pathogenesis of Sengers syndrome.
Title: Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

in contrast to yeast, only a small fraction of Tim9-Tim10a-Tim10b complex is in a stable association with Tim22.
Title: Organization and function of the small Tim complexes acting along the import pathway of metabolite carriers into mammalian mitochondria.

The following TIMM22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIMM22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30247	NM_013337.4 Latest version!	Homo sapiens translocase of inner mitochondrial membrane 22 (TIMM22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu30247	NM_013337.2	Homo sapiens translocase of inner mitochondrial membrane 22 homolog (yeast) (TIMM22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu30247	NM_013337.3	Homo sapiens translocase of inner mitochondrial membrane 22 (TIMM22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIMM22 ( NM_013337.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_037469.2 Homo sapiens translocase of inner mitochondrial membrane 22 (TIMM22), mRNA.

Price & Availability↑

CloneID	OHu30247
Clone ID Related Accession (Same CDS sequence)	NM_013337.4 , NM_013337.2 , NM_013337.3
Accession Version	NM_013337.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	mitochondrial import inner membrane translocase subunit Tim22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002324.2, AI522232.1 and AC015884.15. On Nov 23, 2018 this sequence version replaced NM_013337.3. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.162958.1, SRR1660807.72389.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000327158.5/ ENSP00000320236.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGG CCGCCCCCAA TGCCGGAGGC TCGGCCCCTG AGACAGCGGG TTCCGCCGAA 
GCTCCGCTGC AGTACAGCCT GCTCCTGCAG TACCTGGTGG GTGACAAGCG TCAGCCCCGG 
CTCCTGGAGC CTGGGAGCCT GGGCGGGATC CCAAGTCCAG CCAAGAGTGA GGAGCAGAAG 
ATGATCGAGA AGGCGATGGA AAGCTGCGCT TTCAAGGCTG CGCTGGCCTG CGTGGGAGGA 
TTTGTCTTAG GAGGTGCATT TGGGGTGTTT ACCGCTGGCA TCGATACCAA CGTGGGCTTT 
GACCCTAAGG ATCCTTACCG TACACCGACT GCAAAAGAAG TGCTGAAAGA CATGGGGCAG 
AGAGGAATGT CCTATGCCAA AAATTTCGCC ATTGTGGGAG CCATGTTTTC TTGTACTGAG 
TGTTTGATAG AATCTTACCG GGGAACATCA GACTGGAAGA ACAGTGTCAT CAGTGGCTGC 
ATCACGGGAG GAGCTATTGG TTTCAGAGCT GGCTTAAAGG CTGGGGCCAT TGGTTGTGGA 
GGTTTTGCTG CTTTCTCTGC TGCGATTGAT TATTACCTCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037469.2
CDS	15..599
Translation	MAAAAPNAGGSAPETAGSAEAPLQYSLLLQYLVGDKRQPRLLEPGSLGGIPSPAKSEEQKMIEKAMESCAFKAALACVGGFVLGGAFGVFTAGIDTNVGFDPKDPYRTPTAKEVLKDMGQRGMSYAKNFAIVGAMFSCTECLIESYRGTSDWKNSVISGCITGGAIGFRAGLKAGAIGCGGFAAFSAAIDYYLR

Target ORF information:

RefSeq Version	NM_013337.4
Organism	Homo sapiens(human)
Definition	Homo sapiens translocase of inner mitochondrial membrane 22 (TIMM22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013337.4

ORF Insert Sequence:

ATGGCGGCGG CCGCCCCCAA TGCCGGAGGC TCGGCCCCTG AGACAGCGGG TTCCGCCGAA 
GCTCCGCTGC AGTACAGCCT GCTCCTGCAG TACCTGGTGG GTGACAAGCG TCAGCCCCGG 
CTCCTGGAGC CTGGGAGCCT GGGCGGGATC CCAAGTCCAG CCAAGAGTGA GGAGCAGAAG 
ATGATCGAGA AGGCGATGGA AAGCTGCGCT TTCAAGGCTG CGCTGGCCTG CGTGGGAGGA 
TTTGTCTTAG GAGGTGCATT TGGGGTGTTT ACCGCTGGCA TCGATACCAA CGTGGGCTTT 
GACCCTAAGG ATCCTTACCG TACACCGACT GCAAAAGAAG TGCTGAAAGA CATGGGGCAG 
AGAGGAATGT CCTATGCCAA AAATTTCGCC ATTGTGGGAG CCATGTTTTC TTGTACTGAG 
TGTTTGATAG AATCTTACCG GGGAACATCA GACTGGAAGA ACAGTGTCAT CAGTGGCTGC 
ATCACGGGAG GAGCTATTGG TTTCAGAGCT GGCTTAAAGG CTGGGGCCAT TGGTTGTGGA 
GGTTTTGCTG CTTTCTCTGC TGCGATTGAT TATTACCTCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMM22 ( NM_013337.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_037469.2 Homo sapiens translocase of inner mitochondrial membrane 22 homolog (yeast) (TIMM22), mRNA.

Price & Availability↑

CloneID	OHu30247
Clone ID Related Accession (Same CDS sequence)	NM_013337.4 , NM_013337.2 , NM_013337.3
Accession Version	NM_013337.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	mitochondrial import inner membrane translocase subunit Tim22
Comment

ATGGCGGCGG CCGCCCCCAA TGCCGGAGGC TCGGCCCCTG AGACAGCGGG TTCCGCCGAA 
GCTCCGCTGC AGTACAGCCT GCTCCTGCAG TACCTGGTGG GTGACAAGCG TCAGCCCCGG 
CTCCTGGAGC CTGGGAGCCT GGGCGGGATC CCAAGTCCAG CCAAGAGTGA GGAGCAGAAG 
ATGATCGAGA AGGCGATGGA AAGCTGCGCT TTCAAGGCTG CGCTGGCCTG CGTGGGAGGA 
TTTGTCTTAG GAGGTGCATT TGGGGTGTTT ACCGCTGGCA TCGATACCAA CGTGGGCTTT 
GACCCTAAGG ATCCTTACCG TACACCGACT GCAAAAGAAG TGCTGAAAGA CATGGGGCAG 
AGAGGAATGT CCTATGCCAA AAATTTCGCC ATTGTGGGAG CCATGTTTTC TTGTACTGAG 
TGTTTGATAG AATCTTACCG GGGAACATCA GACTGGAAGA ACAGTGTCAT CAGTGGCTGC 
ATCACGGGAG GAGCTATTGG TTTCAGAGCT GGCTTAAAGG CTGGGGCCAT TGGTTGTGGA 
GGTTTTGCTG CTTTCTCTGC TGCGATTGAT TATTACCTCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037469.2
Translation	MAAAAPNAGGSAPETAGSAEAPLQYSLLLQYLVGDKRQPRLLEPGSLGGIPSPAKSEEQKMIEKAMESCAFKAALACVGGFVLGGAFGVFTAGIDTNVGFDPKDPYRTPTAKEVLKDMGQRGMSYAKNFAIVGAMFSCTECLIESYRGTSDWKNSVISGCITGGAIGFRAGLKAGAIGCGGFAAFSAAIDYYLR

Target ORF information:

RefSeq Version	NM_013337.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens translocase of inner mitochondrial membrane 22 homolog (yeast) (TIMM22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013337.2

ORF Insert Sequence:

ATGGCGGCGG CCGCCCCCAA TGCCGGAGGC TCGGCCCCTG AGACAGCGGG TTCCGCCGAA 
GCTCCGCTGC AGTACAGCCT GCTCCTGCAG TACCTGGTGG GTGACAAGCG TCAGCCCCGG 
CTCCTGGAGC CTGGGAGCCT GGGCGGGATC CCAAGTCCAG CCAAGAGTGA GGAGCAGAAG 
ATGATCGAGA AGGCGATGGA AAGCTGCGCT TTCAAGGCTG CGCTGGCCTG CGTGGGAGGA 
TTTGTCTTAG GAGGTGCATT TGGGGTGTTT ACCGCTGGCA TCGATACCAA CGTGGGCTTT 
GACCCTAAGG ATCCTTACCG TACACCGACT GCAAAAGAAG TGCTGAAAGA CATGGGGCAG 
AGAGGAATGT CCTATGCCAA AAATTTCGCC ATTGTGGGAG CCATGTTTTC TTGTACTGAG 
TGTTTGATAG AATCTTACCG GGGAACATCA GACTGGAAGA ACAGTGTCAT CAGTGGCTGC 
ATCACGGGAG GAGCTATTGG TTTCAGAGCT GGCTTAAAGG CTGGGGCCAT TGGTTGTGGA 
GGTTTTGCTG CTTTCTCTGC TGCGATTGAT TATTACCTCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMM22 ( NM_013337.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_037469.2 Homo sapiens translocase of inner mitochondrial membrane 22 (TIMM22), mRNA.

Price & Availability↑

CloneID	OHu30247
Clone ID Related Accession (Same CDS sequence)	NM_013337.4 , NM_013337.2 , NM_013337.3
Accession Version	NM_013337.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	mitochondrial import inner membrane translocase subunit Tim22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ925032.1, BC002324.2 and AI522232.1. On Jul 30, 2016 this sequence version replaced NM_013337.2. ##Evidence-Data-START## Transcript exon combination :: BQ925032.1, SRR1660807.237442.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CCGCCCCCAA TGCCGGAGGC TCGGCCCCTG AGACAGCGGG TTCCGCCGAA 
GCTCCGCTGC AGTACAGCCT GCTCCTGCAG TACCTGGTGG GTGACAAGCG TCAGCCCCGG 
CTCCTGGAGC CTGGGAGCCT GGGCGGGATC CCAAGTCCAG CCAAGAGTGA GGAGCAGAAG 
ATGATCGAGA AGGCGATGGA AAGCTGCGCT TTCAAGGCTG CGCTGGCCTG CGTGGGAGGA 
TTTGTCTTAG GAGGTGCATT TGGGGTGTTT ACCGCTGGCA TCGATACCAA CGTGGGCTTT 
GACCCTAAGG ATCCTTACCG TACACCGACT GCAAAAGAAG TGCTGAAAGA CATGGGGCAG 
AGAGGAATGT CCTATGCCAA AAATTTCGCC ATTGTGGGAG CCATGTTTTC TTGTACTGAG 
TGTTTGATAG AATCTTACCG GGGAACATCA GACTGGAAGA ACAGTGTCAT CAGTGGCTGC 
ATCACGGGAG GAGCTATTGG TTTCAGAGCT GGCTTAAAGG CTGGGGCCAT TGGTTGTGGA 
GGTTTTGCTG CTTTCTCTGC TGCGATTGAT TATTACCTCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037469.2
CDS	251..835
Translation	MAAAAPNAGGSAPETAGSAEAPLQYSLLLQYLVGDKRQPRLLEPGSLGGIPSPAKSEEQKMIEKAMESCAFKAALACVGGFVLGGAFGVFTAGIDTNVGFDPKDPYRTPTAKEVLKDMGQRGMSYAKNFAIVGAMFSCTECLIESYRGTSDWKNSVISGCITGGAIGFRAGLKAGAIGCGGFAAFSAAIDYYLR

Target ORF information:

RefSeq Version	NM_013337.3
Organism	Homo sapiens(human)
Definition	Homo sapiens translocase of inner mitochondrial membrane 22 (TIMM22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013337.3

ORF Insert Sequence:

ATGGCGGCGG CCGCCCCCAA TGCCGGAGGC TCGGCCCCTG AGACAGCGGG TTCCGCCGAA 
GCTCCGCTGC AGTACAGCCT GCTCCTGCAG TACCTGGTGG GTGACAAGCG TCAGCCCCGG 
CTCCTGGAGC CTGGGAGCCT GGGCGGGATC CCAAGTCCAG CCAAGAGTGA GGAGCAGAAG 
ATGATCGAGA AGGCGATGGA AAGCTGCGCT TTCAAGGCTG CGCTGGCCTG CGTGGGAGGA 
TTTGTCTTAG GAGGTGCATT TGGGGTGTTT ACCGCTGGCA TCGATACCAA CGTGGGCTTT 
GACCCTAAGG ATCCTTACCG TACACCGACT GCAAAAGAAG TGCTGAAAGA CATGGGGCAG 
AGAGGAATGT CCTATGCCAA AAATTTCGCC ATTGTGGGAG CCATGTTTTC TTGTACTGAG 
TGTTTGATAG AATCTTACCG GGGAACATCA GACTGGAAGA ACAGTGTCAT CAGTGGCTGC 
ATCACGGGAG GAGCTATTGG TTTCAGAGCT GGCTTAAAGG CTGGGGCCAT TGGTTGTGGA 
GGTTTTGCTG CTTTCTCTGC TGCGATTGAT TATTACCTCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.
Molecular cell67(3)457-470.e5(2017 Aug)
Kang Y,Stroud DA,Baker MJ,De Souza DP,Frazier AE,Liem M,Tull D,Mathivanan S,McConville MJ,Thorburn DR,Ryan MT,Stojanovski D

Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.
Molecular cell67(3)471-483.e7(2017 Aug)
Vukotic M,Nolte H,K?nig T,Saita S,Ananjew M,Kr?ger M,Tatsuta T,Langer T

TIM29 is a subunit of the human carrier translocase required for protein transport.
FEBS letters590(23)4147-4158(2016 Dec)
Callegari S,Richter F,Chojnacka K,Jans DC,Lorenzi I,Pacheu-Grau D,Jakobs S,Lenz C,Urlaub H,Dudek J,Chacinska A,Rehling P

Organization and function of the small Tim complexes acting along the import pathway of metabolite carriers into mammalian mitochondria.
The Journal of biological chemistry279(14)13540-6(2004 Apr)
M?hlenbein N,Hofmann S,Rothbauer U,Bauer MF

Tim22, the essential core of the mitochondrial protein insertion complex, forms a voltage-activated and signal-gated channel.
Molecular cell9(2)363-73(2002 Feb)
Kovermann P,Truscott KN,Guiard B,Rehling P,Sepuri NB,M?ller H,Jensen RE,Wagner R,Pfanner N

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TIMM22 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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