NPC1L1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001300967.2	NP_001287896.1	NPC1-like intracellular cholesterol transporter 1 isoform 3 precursor
NM_013389.2	NP_037521.2	NPC1-like intracellular cholesterol transporter 1 isoform 1 precursor
XM_011515326.2	XP_011513628.1	Niemann-Pick C1-like protein 1 isoform X1
NM_001101648.2	NP_001095118.1	NPC1-like intracellular cholesterol transporter 1 isoform 2 precursor
NM_001300967.1	NP_001287896.1	NPC1-like intracellular cholesterol transporter 1 isoform 3 precursor
XM_011515327.2	XP_011513629.1	Niemann-Pick C1-like protein 1 isoform X2
XM_011515326.3	XP_011513628.1	NPC1-like intracellular cholesterol transporter 1 isoform X1
NM_001101648.1	NP_001095118.1	NPC1-like intracellular cholesterol transporter 1 isoform 2 precursor
NM_013389.3	NP_037521.2	NPC1-like intracellular cholesterol transporter 1 isoform 1 precursor
XM_011515328.2	XP_011513630.1	NPC1-like intracellular cholesterol transporter 1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24706	NM_013389.3 Latest version!	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu41168	NM_001300967.2 Latest version!	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu67390	XM_011515327.2 Latest version!	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu67391	XM_011515328.2 Latest version!	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu24905	NM_001101648.2 Latest version!	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu67389	XM_011515326.3 Latest version!	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu41168	NM_001300967.1	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu67389	XM_011515326.2	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu24905	NM_001101648.1	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu24706	NM_013389.2	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
Hide Old Accession Versions >>

NPC1L1 ( NM_013389.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_037521.2 Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu24706
Clone ID Related Accession (Same CDS sequence)	NM_013389.2 , NM_013389.3
Accession Version	NM_013389.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Homo sapiens(human)
Product	NPC1-like intracellular cholesterol transporter 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF192522.1 and AC004938.3. This sequence is a reference standard in the RefSeqGene project. On Aug 23, 2007 this sequence version replaced NM_013389.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF192522.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142586 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGAGGC TGCCCCGGAG GCCTGGGGAG CCACGAGAGG TCCACATTGG GCGAGCCCTA 
GGCAGGGTGG CTCCCAGCAT GCTGTTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTCCTA 
GGGGCCCTGA CCCCCATGCC AGCTGTGCGG ACCTTTGCCC TGACCTCTGG CCTTGCAGTG 
ATCCTTGACT TCCTCCTGCA GATGTCAGCC TTTGTGGCCC TGCTCTCCCT GGACAGCAAG 
AGGCAGGAGG CCTCCCGGTT GGACGTCTGC TGCTGTGTCA AGCCCCAGGA GCTGCCCCCG 
CCTGGCCAGG GAGAGGGGCT CCTGCTTGGC TTCTTCCAAA AGGCTTATGC CCCCTTCCTG 
CTGCACTGGA TCACTCGAGG TGTTGTGCTG CTGCTGTTTC TCGCCCTGTT CGGAGTGAGC 
CTCTACTCCA TGTGCCACAT CAGCGTGGGA CTGGACCAGG AGCTGGCCCT GCCCAAGGAC 
TCGTACCTGC TTGACTATTT CCTCTTTCTG AACCGCTACT TCGAGGTGGG GGCCCCGGTG 
TACTTTGTTA CCACCTTGGG CTACAACTTC TCCAGCGAGG CTGGGATGAA TGCCATCTGC 
TCCAGTGCAG GCTGCAACAA CTTCTCCTTC ACCCAGAAGA TCCAGTATGC CACAGAGTTC 
CCTGAGCAGT CTTACCTGGC CATCCCTGCC TCCTCCTGGG TGGATGACTT CATTGACTGG 
CTGACCCCGT CCTCCTGCTG CCGCCTTTAT ATATCTGGCC CCAATAAGGA CAAGTTCTGC 
CCCTCGACCG TCAACTCTCT GAACTGCCTA AAGAACTGCA TGAGCATCAC GATGGGCTCT 
GTGAGGCCCT CGGTGGAGCA GTTCCATAAG TATCTTCCCT GGTTCCTGAA CGACCGGCCC 
AACATCAAAT GTCCCAAAGG CGGCCTGGCA GCATACAGCA CCTCTGTGAA CTTGACTTCA 
GATGGCCAGG TTTTAGACAC AGTTGCCATT CTGTCACCCA GGCTGGAGTA CAGTGGCACA 
ATCTCGGCTC ACTGCAACCT CTACCTCCTG GATTCAACCT CCAGGTTCAT GGCCTATCAC 
AAGCCCCTGA AAAACTCACA GGATTACACA GAAGCTCTGC GGGCAGCTCG AGAGCTGGCA 
GCCAACATCA CTGCTGACCT GCGGAAAGTG CCTGGAACAG ACCCGGCTTT TGAGGTCTTC 
CCCTACACGA TCACCAATGT GTTTTATGAG CAGTACCTGA CCATCCTCCC TGAGGGGCTC 
TTCATGCTCA GCCTCTGCCT TGTGCCCACC TTCGCTGTCT CCTGCCTCCT GCTGGGCCTG 
GACCTGCGCT CCGGCCTCCT CAACCTGCTC TCCATTGTCA TGATCCTCGT GGACACTGTC 
GGCTTCATGG CCCTGTGGGG CATCAGTTAC AATGCTGTGT CCCTCATCAA CCTGGTCTCG 
GCGGTGGGCA TGTCTGTGGA GTTTGTGTCC CACATTACCC GCTCCTTTGC CATCAGCACC 
AAGCCCACCT GGCTGGAGAG GGCCAAAGAG GCCACCATCT CTATGGGAAG TGCGGTGTTT 
GCAGGTGTGG CCATGACCAA CCTGCCTGGC ATCCTTGTCC TGGGCCTCGC CAAGGCCCAG 
CTCATTCAGA TCTTCTTCTT CCGCCTCAAC CTCCTGATCA CTCTGCTGGG CCTGCTGCAT 
GGCTTGGTCT TCCTGCCCGT CATCCTCAGC TACGTGGGGC CTGACGTTAA CCCGGCTCTG 
GCACTGGAGC AGAAGCGGGC TGAGGAGGCG GTGGCAGCAG TCATGGTGGC CTCTTGCCCA 
AATCACCCCT CCCGAGTCTC CACAGCTGAC AACATCTATG TCAACCACAG CTTTGAAGGT 
TCTATCAAAG GTGCTGGTGC CATCAGCAAC TTCTTGCCCA ACAATGGGCG GCAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037521.2
CDS	57..4136
Translation	MAEAGLRGWLLWALLLRLAQSEPYTTIHQPGYCAFYDECGKNPELSGSLMTLSNVSCLSNTPARKITGDHLILLQKICPRLYTGPNTQACCSAKQLVSLEASLSITKALLTRCPACSDNFVNLHCHNTCSPNQSLFINVTRVAQLGAGQLPAVVAYEAFYQHSFAEQSYDSCSRVRVPAAATLAVGTMCGVYGSALCNAQRWLNFQGDTGNGLAPLDITFHLLEPGQAVGSGIQPLNEGVARCNESQGDDVATCSCQDCAASCPAIARPQALDSTFYLGQMPGSLVLIIILCSVFAVVTILLVGFRVAPARDKSKMVDPKKGTSLSDKLSFSTHTLLGQFFQGWGTWVASWPLTILVLSVIPVVALAAGLVFTELTTDPVELWSAPNSQARSEKAFHDQHFGPFFRTNQVILTAPNRSSYRYDSLLLGPKNFSGILDLDLLLELLELQERLRHLQVWSPEAQRNISLQDICYAPLNPDNTSLYDCCINSLLQYFQNNRTLLLLTANQTLMGQTSQVDWKDHFLYCANAPLTFKDGTALALSCMADYGAPVFPFLAIGGYKGKDYSEAEALIMTFSLNNYPAGDPRLAQAKLWEEAFLEEMRAFQRRMAGMFQVTFMAERSLEDEINRTTAEDLPIFATSYIVIFLYISLALGSYSSWSRVMVDSKATLGLGGVAVVLGAVMAAMGFFSYLGIRSSLVILQVVPFLVLSVGADNIFIFVLEYQRLPRRPGEPREVHIGRALGRVAPSMLLCSLSEAICFFLGALTPMPAVRTFALTSGLAVILDFLLQMSAFVALLSLDSKRQEASRLDVCCCVKPQELPPPGQGEGLLLGFFQKAYAPFLLHWITRGVVLLLFLALFGVSLYSMCHISVGLDQELALPKDSYLLDYFLFLNRYFEVGAPVYFVTTLGYNFSSEAGMNAICSSAGCNNFSFTQKIQYATEFPEQSYLAIPASSWVDDFIDWLTPSSCCRLYISGPNKDKFCPSTVNSLNCLKNCMSITMGSVRPSVEQFHKYLPWFLNDRPNIKCPKGGLAAYSTSVNLTSDGQVLDTVAILSPRLEYSGTISAHCNLYLLDSTSRFMAYHKPLKNSQDYTEALRAARELAANITADLRKVPGTDPAFEVFPYTITNVFYEQYLTILPEGLFMLSLCLVPTFAVSCLLLGLDLRSGLLNLLSIVMILVDTVGFMALWGISYNAVSLINLVSAVGMSVEFVSHITRSFAISTKPTWLERAKEATISMGSAVFAGVAMTNLPGILVLGLAKAQLIQIFFFRLNLLITLLGLLHGLVFLPVILSYVGPDVNPALALEQKRAEEAVAAVMVASCPNHPSRVSTADNIYVNHSFEGSIKGAGAISNFLPNNGRQF

Target ORF information:

RefSeq Version	NM_013389.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013389.2

ORF Insert Sequence:

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGAGGC TGCCCCGGAG GCCTGGGGAG CCACGAGAGG TCCACATTGG GCGAGCCCTA 
GGCAGGGTGG CTCCCAGCAT GCTGTTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTCCTA 
GGGGCCCTGA CCCCCATGCC AGCTGTGCGG ACCTTTGCCC TGACCTCTGG CCTTGCAGTG 
ATCCTTGACT TCCTCCTGCA GATGTCAGCC TTTGTGGCCC TGCTCTCCCT GGACAGCAAG 
AGGCAGGAGG CCTCCCGGTT GGACGTCTGC TGCTGTGTCA AGCCCCAGGA GCTGCCCCCG 
CCTGGCCAGG GAGAGGGGCT CCTGCTTGGC TTCTTCCAAA AGGCTTATGC CCCCTTCCTG 
CTGCACTGGA TCACTCGAGG TGTTGTGCTG CTGCTGTTTC TCGCCCTGTT CGGAGTGAGC 
CTCTACTCCA TGTGCCACAT CAGCGTGGGA CTGGACCAGG AGCTGGCCCT GCCCAAGGAC 
TCGTACCTGC TTGACTATTT CCTCTTTCTG AACCGCTACT TCGAGGTGGG GGCCCCGGTG 
TACTTTGTTA CCACCTTGGG CTACAACTTC TCCAGCGAGG CTGGGATGAA TGCCATCTGC 
TCCAGTGCAG GCTGCAACAA CTTCTCCTTC ACCCAGAAGA TCCAGTATGC CACAGAGTTC 
CCTGAGCAGT CTTACCTGGC CATCCCTGCC TCCTCCTGGG TGGATGACTT CATTGACTGG 
CTGACCCCGT CCTCCTGCTG CCGCCTTTAT ATATCTGGCC CCAATAAGGA CAAGTTCTGC 
CCCTCGACCG TCAACTCTCT GAACTGCCTA AAGAACTGCA TGAGCATCAC GATGGGCTCT 
GTGAGGCCCT CGGTGGAGCA GTTCCATAAG TATCTTCCCT GGTTCCTGAA CGACCGGCCC 
AACATCAAAT GTCCCAAAGG CGGCCTGGCA GCATACAGCA CCTCTGTGAA CTTGACTTCA 
GATGGCCAGG TTTTAGACAC AGTTGCCATT CTGTCACCCA GGCTGGAGTA CAGTGGCACA 
ATCTCGGCTC ACTGCAACCT CTACCTCCTG GATTCAACCT CCAGGTTCAT GGCCTATCAC 
AAGCCCCTGA AAAACTCACA GGATTACACA GAAGCTCTGC GGGCAGCTCG AGAGCTGGCA 
GCCAACATCA CTGCTGACCT GCGGAAAGTG CCTGGAACAG ACCCGGCTTT TGAGGTCTTC 
CCCTACACGA TCACCAATGT GTTTTATGAG CAGTACCTGA CCATCCTCCC TGAGGGGCTC 
TTCATGCTCA GCCTCTGCCT TGTGCCCACC TTCGCTGTCT CCTGCCTCCT GCTGGGCCTG 
GACCTGCGCT CCGGCCTCCT CAACCTGCTC TCCATTGTCA TGATCCTCGT GGACACTGTC 
GGCTTCATGG CCCTGTGGGG CATCAGTTAC AATGCTGTGT CCCTCATCAA CCTGGTCTCG 
GCGGTGGGCA TGTCTGTGGA GTTTGTGTCC CACATTACCC GCTCCTTTGC CATCAGCACC 
AAGCCCACCT GGCTGGAGAG GGCCAAAGAG GCCACCATCT CTATGGGAAG TGCGGTGTTT 
GCAGGTGTGG CCATGACCAA CCTGCCTGGC ATCCTTGTCC TGGGCCTCGC CAAGGCCCAG 
CTCATTCAGA TCTTCTTCTT CCGCCTCAAC CTCCTGATCA CTCTGCTGGG CCTGCTGCAT 
GGCTTGGTCT TCCTGCCCGT CATCCTCAGC TACGTGGGGC CTGACGTTAA CCCGGCTCTG 
GCACTGGAGC AGAAGCGGGC TGAGGAGGCG GTGGCAGCAG TCATGGTGGC CTCTTGCCCA 
AATCACCCCT CCCGAGTCTC CACAGCTGAC AACATCTATG TCAACCACAG CTTTGAAGGT 
TCTATCAAAG GTGCTGGTGC CATCAGCAAC TTCTTGCCCA ACAATGGGCG GCAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPC1L1 ( NM_013389.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_037521.2 Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu24706
Clone ID Related Accession (Same CDS sequence)	NM_013389.2 , NM_013389.3
Accession Version	NM_013389.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	NPC1-like intracellular cholesterol transporter 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004938.3 and AF192522.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_013389.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF192522.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142586 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGAGGC TGCCCCGGAG GCCTGGGGAG CCACGAGAGG TCCACATTGG GCGAGCCCTA 
GGCAGGGTGG CTCCCAGCAT GCTGTTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTCCTA 
GGGGCCCTGA CCCCCATGCC AGCTGTGCGG ACCTTTGCCC TGACCTCTGG CCTTGCAGTG 
ATCCTTGACT TCCTCCTGCA GATGTCAGCC TTTGTGGCCC TGCTCTCCCT GGACAGCAAG 
AGGCAGGAGG CCTCCCGGTT GGACGTCTGC TGCTGTGTCA AGCCCCAGGA GCTGCCCCCG 
CCTGGCCAGG GAGAGGGGCT CCTGCTTGGC TTCTTCCAAA AGGCTTATGC CCCCTTCCTG 
CTGCACTGGA TCACTCGAGG TGTTGTGCTG CTGCTGTTTC TCGCCCTGTT CGGAGTGAGC 
CTCTACTCCA TGTGCCACAT CAGCGTGGGA CTGGACCAGG AGCTGGCCCT GCCCAAGGAC 
TCGTACCTGC TTGACTATTT CCTCTTTCTG AACCGCTACT TCGAGGTGGG GGCCCCGGTG 
TACTTTGTTA CCACCTTGGG CTACAACTTC TCCAGCGAGG CTGGGATGAA TGCCATCTGC 
TCCAGTGCAG GCTGCAACAA CTTCTCCTTC ACCCAGAAGA TCCAGTATGC CACAGAGTTC 
CCTGAGCAGT CTTACCTGGC CATCCCTGCC TCCTCCTGGG TGGATGACTT CATTGACTGG 
CTGACCCCGT CCTCCTGCTG CCGCCTTTAT ATATCTGGCC CCAATAAGGA CAAGTTCTGC 
CCCTCGACCG TCAACTCTCT GAACTGCCTA AAGAACTGCA TGAGCATCAC GATGGGCTCT 
GTGAGGCCCT CGGTGGAGCA GTTCCATAAG TATCTTCCCT GGTTCCTGAA CGACCGGCCC 
AACATCAAAT GTCCCAAAGG CGGCCTGGCA GCATACAGCA CCTCTGTGAA CTTGACTTCA 
GATGGCCAGG TTTTAGACAC AGTTGCCATT CTGTCACCCA GGCTGGAGTA CAGTGGCACA 
ATCTCGGCTC ACTGCAACCT CTACCTCCTG GATTCAACCT CCAGGTTCAT GGCCTATCAC 
AAGCCCCTGA AAAACTCACA GGATTACACA GAAGCTCTGC GGGCAGCTCG AGAGCTGGCA 
GCCAACATCA CTGCTGACCT GCGGAAAGTG CCTGGAACAG ACCCGGCTTT TGAGGTCTTC 
CCCTACACGA TCACCAATGT GTTTTATGAG CAGTACCTGA CCATCCTCCC TGAGGGGCTC 
TTCATGCTCA GCCTCTGCCT TGTGCCCACC TTCGCTGTCT CCTGCCTCCT GCTGGGCCTG 
GACCTGCGCT CCGGCCTCCT CAACCTGCTC TCCATTGTCA TGATCCTCGT GGACACTGTC 
GGCTTCATGG CCCTGTGGGG CATCAGTTAC AATGCTGTGT CCCTCATCAA CCTGGTCTCG 
GCGGTGGGCA TGTCTGTGGA GTTTGTGTCC CACATTACCC GCTCCTTTGC CATCAGCACC 
AAGCCCACCT GGCTGGAGAG GGCCAAAGAG GCCACCATCT CTATGGGAAG TGCGGTGTTT 
GCAGGTGTGG CCATGACCAA CCTGCCTGGC ATCCTTGTCC TGGGCCTCGC CAAGGCCCAG 
CTCATTCAGA TCTTCTTCTT CCGCCTCAAC CTCCTGATCA CTCTGCTGGG CCTGCTGCAT 
GGCTTGGTCT TCCTGCCCGT CATCCTCAGC TACGTGGGGC CTGACGTTAA CCCGGCTCTG 
GCACTGGAGC AGAAGCGGGC TGAGGAGGCG GTGGCAGCAG TCATGGTGGC CTCTTGCCCA 
AATCACCCCT CCCGAGTCTC CACAGCTGAC AACATCTATG TCAACCACAG CTTTGAAGGT 
TCTATCAAAG GTGCTGGTGC CATCAGCAAC TTCTTGCCCA ACAATGGGCG GCAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037521.2
CDS	72..4151
Translation	MAEAGLRGWLLWALLLRLAQSEPYTTIHQPGYCAFYDECGKNPELSGSLMTLSNVSCLSNTPARKITGDHLILLQKICPRLYTGPNTQACCSAKQLVSLEASLSITKALLTRCPACSDNFVNLHCHNTCSPNQSLFINVTRVAQLGAGQLPAVVAYEAFYQHSFAEQSYDSCSRVRVPAAATLAVGTMCGVYGSALCNAQRWLNFQGDTGNGLAPLDITFHLLEPGQAVGSGIQPLNEGVARCNESQGDDVATCSCQDCAASCPAIARPQALDSTFYLGQMPGSLVLIIILCSVFAVVTILLVGFRVAPARDKSKMVDPKKGTSLSDKLSFSTHTLLGQFFQGWGTWVASWPLTILVLSVIPVVALAAGLVFTELTTDPVELWSAPNSQARSEKAFHDQHFGPFFRTNQVILTAPNRSSYRYDSLLLGPKNFSGILDLDLLLELLELQERLRHLQVWSPEAQRNISLQDICYAPLNPDNTSLYDCCINSLLQYFQNNRTLLLLTANQTLMGQTSQVDWKDHFLYCANAPLTFKDGTALALSCMADYGAPVFPFLAIGGYKGKDYSEAEALIMTFSLNNYPAGDPRLAQAKLWEEAFLEEMRAFQRRMAGMFQVTFMAERSLEDEINRTTAEDLPIFATSYIVIFLYISLALGSYSSWSRVMVDSKATLGLGGVAVVLGAVMAAMGFFSYLGIRSSLVILQVVPFLVLSVGADNIFIFVLEYQRLPRRPGEPREVHIGRALGRVAPSMLLCSLSEAICFFLGALTPMPAVRTFALTSGLAVILDFLLQMSAFVALLSLDSKRQEASRLDVCCCVKPQELPPPGQGEGLLLGFFQKAYAPFLLHWITRGVVLLLFLALFGVSLYSMCHISVGLDQELALPKDSYLLDYFLFLNRYFEVGAPVYFVTTLGYNFSSEAGMNAICSSAGCNNFSFTQKIQYATEFPEQSYLAIPASSWVDDFIDWLTPSSCCRLYISGPNKDKFCPSTVNSLNCLKNCMSITMGSVRPSVEQFHKYLPWFLNDRPNIKCPKGGLAAYSTSVNLTSDGQVLDTVAILSPRLEYSGTISAHCNLYLLDSTSRFMAYHKPLKNSQDYTEALRAARELAANITADLRKVPGTDPAFEVFPYTITNVFYEQYLTILPEGLFMLSLCLVPTFAVSCLLLGLDLRSGLLNLLSIVMILVDTVGFMALWGISYNAVSLINLVSAVGMSVEFVSHITRSFAISTKPTWLERAKEATISMGSAVFAGVAMTNLPGILVLGLAKAQLIQIFFFRLNLLITLLGLLHGLVFLPVILSYVGPDVNPALALEQKRAEEAVAAVMVASCPNHPSRVSTADNIYVNHSFEGSIKGAGAISNFLPNNGRQF

Target ORF information:

RefSeq Version	NM_013389.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013389.3

ORF Insert Sequence:

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGAGGC TGCCCCGGAG GCCTGGGGAG CCACGAGAGG TCCACATTGG GCGAGCCCTA 
GGCAGGGTGG CTCCCAGCAT GCTGTTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTCCTA 
GGGGCCCTGA CCCCCATGCC AGCTGTGCGG ACCTTTGCCC TGACCTCTGG CCTTGCAGTG 
ATCCTTGACT TCCTCCTGCA GATGTCAGCC TTTGTGGCCC TGCTCTCCCT GGACAGCAAG 
AGGCAGGAGG CCTCCCGGTT GGACGTCTGC TGCTGTGTCA AGCCCCAGGA GCTGCCCCCG 
CCTGGCCAGG GAGAGGGGCT CCTGCTTGGC TTCTTCCAAA AGGCTTATGC CCCCTTCCTG 
CTGCACTGGA TCACTCGAGG TGTTGTGCTG CTGCTGTTTC TCGCCCTGTT CGGAGTGAGC 
CTCTACTCCA TGTGCCACAT CAGCGTGGGA CTGGACCAGG AGCTGGCCCT GCCCAAGGAC 
TCGTACCTGC TTGACTATTT CCTCTTTCTG AACCGCTACT TCGAGGTGGG GGCCCCGGTG 
TACTTTGTTA CCACCTTGGG CTACAACTTC TCCAGCGAGG CTGGGATGAA TGCCATCTGC 
TCCAGTGCAG GCTGCAACAA CTTCTCCTTC ACCCAGAAGA TCCAGTATGC CACAGAGTTC 
CCTGAGCAGT CTTACCTGGC CATCCCTGCC TCCTCCTGGG TGGATGACTT CATTGACTGG 
CTGACCCCGT CCTCCTGCTG CCGCCTTTAT ATATCTGGCC CCAATAAGGA CAAGTTCTGC 
CCCTCGACCG TCAACTCTCT GAACTGCCTA AAGAACTGCA TGAGCATCAC GATGGGCTCT 
GTGAGGCCCT CGGTGGAGCA GTTCCATAAG TATCTTCCCT GGTTCCTGAA CGACCGGCCC 
AACATCAAAT GTCCCAAAGG CGGCCTGGCA GCATACAGCA CCTCTGTGAA CTTGACTTCA 
GATGGCCAGG TTTTAGACAC AGTTGCCATT CTGTCACCCA GGCTGGAGTA CAGTGGCACA 
ATCTCGGCTC ACTGCAACCT CTACCTCCTG GATTCAACCT CCAGGTTCAT GGCCTATCAC 
AAGCCCCTGA AAAACTCACA GGATTACACA GAAGCTCTGC GGGCAGCTCG AGAGCTGGCA 
GCCAACATCA CTGCTGACCT GCGGAAAGTG CCTGGAACAG ACCCGGCTTT TGAGGTCTTC 
CCCTACACGA TCACCAATGT GTTTTATGAG CAGTACCTGA CCATCCTCCC TGAGGGGCTC 
TTCATGCTCA GCCTCTGCCT TGTGCCCACC TTCGCTGTCT CCTGCCTCCT GCTGGGCCTG 
GACCTGCGCT CCGGCCTCCT CAACCTGCTC TCCATTGTCA TGATCCTCGT GGACACTGTC 
GGCTTCATGG CCCTGTGGGG CATCAGTTAC AATGCTGTGT CCCTCATCAA CCTGGTCTCG 
GCGGTGGGCA TGTCTGTGGA GTTTGTGTCC CACATTACCC GCTCCTTTGC CATCAGCACC 
AAGCCCACCT GGCTGGAGAG GGCCAAAGAG GCCACCATCT CTATGGGAAG TGCGGTGTTT 
GCAGGTGTGG CCATGACCAA CCTGCCTGGC ATCCTTGTCC TGGGCCTCGC CAAGGCCCAG 
CTCATTCAGA TCTTCTTCTT CCGCCTCAAC CTCCTGATCA CTCTGCTGGG CCTGCTGCAT 
GGCTTGGTCT TCCTGCCCGT CATCCTCAGC TACGTGGGGC CTGACGTTAA CCCGGCTCTG 
GCACTGGAGC AGAAGCGGGC TGAGGAGGCG GTGGCAGCAG TCATGGTGGC CTCTTGCCCA 
AATCACCCCT CCCGAGTCTC CACAGCTGAC AACATCTATG TCAACCACAG CTTTGAAGGT 
TCTATCAAAG GTGCTGGTGC CATCAGCAAC TTCTTGCCCA ACAATGGGCG GCAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPC1L1 ( NM_001300967.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287896.1 Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 3, mRNA.

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CloneID	OHu41168
Clone ID Related Accession (Same CDS sequence)	NM_001300967.2 , NM_001300967.1
Accession Version	NM_001300967.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	NPC1-like intracellular cholesterol transporter 1 isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004938.3 and AF192523.1. On Jun 2, 2019 this sequence version replaced NM_001300967.1. Transcript Variant: This variant (3) lacks several exons and its 3'-terminal exon extends past a splice site that is used in variant 1. The encoded isoform (3) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF192523.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGGGGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287896.1
CDS	72..2246
Translation	MAEAGLRGWLLWALLLRLAQSEPYTTIHQPGYCAFYDECGKNPELSGSLMTLSNVSCLSNTPARKITGDHLILLQKICPRLYTGPNTQACCSAKQLVSLEASLSITKALLTRCPACSDNFVNLHCHNTCSPNQSLFINVTRVAQLGAGQLPAVVAYEAFYQHSFAEQSYDSCSRVRVPAAATLAVGTMCGVYGSALCNAQRWLNFQGDTGNGLAPLDITFHLLEPGQAVGSGIQPLNEGVARCNESQGDDVATCSCQDCAASCPAIARPQALDSTFYLGQMPGSLVLIIILCSVFAVVTILLVGFRVAPARDKSKMVDPKKGTSLSDKLSFSTHTLLGQFFQGWGTWVASWPLTILVLSVIPVVALAAGLVFTELTTDPVELWSAPNSQARSEKAFHDQHFGPFFRTNQVILTAPNRSSYRYDSLLLGPKNFSGILDLDLLLELLELQERLRHLQVWSPEAQRNISLQDICYAPLNPDNTSLYDCCINSLLQYFQNNRTLLLLTANQTLMGQTSQVDWKDHFLYCANAPLTFKDGTALALSCMADYGAPVFPFLAIGGYKGKDYSEAEALIMTFSLNNYPAGDPRLAQAKLWEEAFLEEMRAFQRRMAGMFQVTFMAERSLEDEINRTTAEDLPIFATSYIVIFLYISLALGSYSSWSRVMVDSKATLGLGGVAVVLGAVMAAMGFFSYLGIRSSLVILQVVPFLVLSVGADNIFIFVLEYQGP

Target ORF information:

RefSeq Version	NM_001300967.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300967.2

ORF Insert Sequence:

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGGGGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPC1L1 ( NM_001300967.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287896.1 Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 3, mRNA.

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CloneID	OHu41168
Clone ID Related Accession (Same CDS sequence)	NM_001300967.2 , NM_001300967.1
Accession Version	NM_001300967.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Homo sapiens(human)
Product	NPC1-like intracellular cholesterol transporter 1 isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF192523.1. On Jul 17, 2014 this sequence version replaced XM_006715709.1. Transcript Variant: This variant (3) lacks several exons and its 3'-terminal exon extends past a splice site that is used in variant 1. The encoded isoform (3) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF192523.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGGGGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287896.1
CDS	57..2231
Translation	MAEAGLRGWLLWALLLRLAQSEPYTTIHQPGYCAFYDECGKNPELSGSLMTLSNVSCLSNTPARKITGDHLILLQKICPRLYTGPNTQACCSAKQLVSLEASLSITKALLTRCPACSDNFVNLHCHNTCSPNQSLFINVTRVAQLGAGQLPAVVAYEAFYQHSFAEQSYDSCSRVRVPAAATLAVGTMCGVYGSALCNAQRWLNFQGDTGNGLAPLDITFHLLEPGQAVGSGIQPLNEGVARCNESQGDDVATCSCQDCAASCPAIARPQALDSTFYLGQMPGSLVLIIILCSVFAVVTILLVGFRVAPARDKSKMVDPKKGTSLSDKLSFSTHTLLGQFFQGWGTWVASWPLTILVLSVIPVVALAAGLVFTELTTDPVELWSAPNSQARSEKAFHDQHFGPFFRTNQVILTAPNRSSYRYDSLLLGPKNFSGILDLDLLLELLELQERLRHLQVWSPEAQRNISLQDICYAPLNPDNTSLYDCCINSLLQYFQNNRTLLLLTANQTLMGQTSQVDWKDHFLYCANAPLTFKDGTALALSCMADYGAPVFPFLAIGGYKGKDYSEAEALIMTFSLNNYPAGDPRLAQAKLWEEAFLEEMRAFQRRMAGMFQVTFMAERSLEDEINRTTAEDLPIFATSYIVIFLYISLALGSYSSWSRVMVDSKATLGLGGVAVVLGAVMAAMGFFSYLGIRSSLVILQVVPFLVLSVGADNIFIFVLEYQGP

Target ORF information:

RefSeq Version	NM_001300967.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300967.1

ORF Insert Sequence:

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGGGGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPC1L1 ( XM_011515327.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513629.1 Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant X2, mRNA.

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CloneID	OHu67390
Clone ID Related Accession (Same CDS sequence)	XM_011515327.2
Accession Version	XM_011515327.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	Niemann-Pick C1-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515327.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGAGGC TGCCCCGGAG GCCTGGGGAG CCACGAGAGG TCCACATTGG GCGAGCCCTA 
GGCAGGGTGG CTCCCAGCAT GCTGTTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTCCTA 
GATGTCAGCC TTTGTGGCCC TGCTCTCCCT GGACAGCAAG AGGCAGGAGG CCTCCCGGTT 
GGACGTCTGC TGCTGTGTCA AGCCCCAGGA GCTGCCCCCG CCTGGCCAGG GAGAGGGGCT 
CCTGCTTGGC TTCTTCCAAA AGGCTTATGC CCCCTTCCTG CTGCACTGGA TCACTCGAGG 
TGTTGTGCTG CTGCTGTTTC TCGCCCTGTT CGGAGTGAGC CTCTACTCCA TGTGCCACAT 
CAGCGTGGGA CTGGACCAGG AGCTGGCCCT GCCCAAGGAC TCGTACCTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513629.1
CDS	392..2965
Translation	MAEAGLRGWLLWALLLRLAQSEPYTTIHQPGYCAFYDECGKNPELSGSLMTLSNVSCLSNTPARKITGDHLILLQKICPRLYTGPNTQACCSAKQLVSLEASLSITKALLTRCPACSDNFVNLHCHNTCSPNQSLFINVTRVAQLGAGQLPAVVAYEAFYQHSFAEQSYDSCSRVRVPAAATLAVGTMCGVYGSALCNAQRWLNFQGDTGNGLAPLDITFHLLEPGQAVGSGIQPLNEGVARCNESQGDDVATCSCQDCAASCPAIARPQALDSTFYLGQMPGSLVLIIILCSVFAVVTILLVGFRVAPARDKSKMVDPKKGTSLSDKLSFSTHTLLGQFFQGWGTWVASWPLTILVLSVIPVVALAAGLVFTELTTDPVELWSAPNSQARSEKAFHDQHFGPFFRTNQVILTAPNRSSYRYDSLLLGPKNFSGILDLDLLLELLELQERLRHLQVWSPEAQRNISLQDICYAPLNPDNTSLYDCCINSLLQYFQNNRTLLLLTANQTLMGQTSQVDWKDHFLYCANAPLTFKDGTALALSCMADYGAPVFPFLAIGGYKGKDYSEAEALIMTFSLNNYPAGDPRLAQAKLWEEAFLEEMRAFQRRMAGMFQVTFMAERSLEDEINRTTAEDLPIFATSYIVIFLYISLALGSYSSWSRVMVDSKATLGLGGVAVVLGAVMAAMGFFSYLGIRSSLVILQVVPFLVLSVGADNIFIFVLEYQRLPRRPGEPREVHIGRALGRVAPSMLLCSLSEAICFFLDVSLCGPALPGQQEAGGLPVGRLLLCQAPGAAPAWPGRGAPAWLLPKGLCPLPAALDHSRCCAAAVSRPVRSEPLLHVPHQRGTGPGAGPAQGLVPA

Target ORF information:

RefSeq Version	XM_011515327.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515327.2

ORF Insert Sequence:

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGAGGC TGCCCCGGAG GCCTGGGGAG CCACGAGAGG TCCACATTGG GCGAGCCCTA 
GGCAGGGTGG CTCCCAGCAT GCTGTTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTCCTA 
GATGTCAGCC TTTGTGGCCC TGCTCTCCCT GGACAGCAAG AGGCAGGAGG CCTCCCGGTT 
GGACGTCTGC TGCTGTGTCA AGCCCCAGGA GCTGCCCCCG CCTGGCCAGG GAGAGGGGCT 
CCTGCTTGGC TTCTTCCAAA AGGCTTATGC CCCCTTCCTG CTGCACTGGA TCACTCGAGG 
TGTTGTGCTG CTGCTGTTTC TCGCCCTGTT CGGAGTGAGC CTCTACTCCA TGTGCCACAT 
CAGCGTGGGA CTGGACCAGG AGCTGGCCCT GCCCAAGGAC TCGTACCTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPC1L1 ( XM_011515328.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513630.1 Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu67391
Clone ID Related Accession (Same CDS sequence)	XM_011515328.2
Accession Version	XM_011515328.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NPC1-like intracellular cholesterol transporter 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCGCCC GAAGCACAGC GCAACATCTC CCTGCAGGAC ATCTGCTACG CCCCCCTCAA 
TCCGGACAAT ACCAGTCTCT ACGACTGCTG CATCAACAGC CTCCTGCAGT ATTTCCAGAA 
CAACCGCACG CTCCTGCTGC TCACAGCCAA CCAGACACTG ATGGGGCAGA CCTCCCAAGT 
CGACTGGAAG GACCATTTTC TGTACTGTGC CAACGCTCTC TGGAAGACGA GATCAATCGC 
ACCACAGCTG AAGACCTGCC CATCTTTGCC ACCAGCTACA TTGTCATATT CCTGTACATC 
TCTCTGGCCC TGGGCAGCTA TTCCAGCTGG AGCCGAGTGA TGGTGGACTC CAAGGCCACG 
CTGGGCCTCG GCGGGGTGGC CGTGGTCCTG GGAGCAGTCA TGGCTGCCAT GGGCTTCTTC 
TCCTACTTGG GTATCCGCTC CTCCCTGGTC ATCCTGCAAG TGGTTCCTTT CCTGGTGCTG 
TCCGTGGGGG CTGATAACAT CTTCATCTTT GTTCTCGAGT ACCAGAGGCT GCCCCGGAGG 
CCTGGGGAGC CACGAGAGGT CCACATTGGG CGAGCCCTAG GCAGGGTGGC TCCCAGCATG 
CTGTTGTGCA GCCTCTCTGA GGCCATCTGC TTCTTCCTAG GGGCCCTGAC CCCCATGCCA 
GCTGTGCGGA CCTTTGCCCT GACCTCTGGC CTTGCAGTGA TCCTTGACTT CCTCCTGCAG 
ATGTCAGCCT TTGTGGCCCT GCTCTCCCTG GACAGCAAGA GGCAGGAGGC CTCCCGGTTG 
GACGTCTGCT GCTGTGTCAA GCCCCAGGAG CTGCCCCCGC CTGGCCAGGG AGAGGGGCTC 
CTGCTTGGCT TCTTCCAAAA GGCTTATGCC CCCTTCCTGC TGCACTGGAT CACTCGAGGT 
GTTGTGCTGC TGCTGTTTCT CGCCCTGTTC GGAGTGAGCC TCTACTCCAT GTGCCACATC 
AGCGTGGGAC TGGACCAGGA GCTGGCCCTG CCCAAGGACT CGTACCTGCT TGACTATTTC 
CTCTTTCTGA ACCGCTACTT CGAGGTGGGG GCCCCGGTGT ACTTTGTTAC CACCTTGGGC 
TACAACTTCT CCAGCGAGGC TGGGATGAAT GCCATCTGCT CCAGTGCAGG CTGCAACAAC 
TTCTCCTTCA CCCAGAAGAT CCAGTATGCC ACAGAGTTCC CTGAGCAGTC TTACCTGGCC 
ATCCCTGCCT CCTCCTGGGT GGATGACTTC ATTGACTGGC TGACCCCGTC CTCCTGCTGC 
CGCCTTTATA TATCTGGCCC CAATAAGGAC AAGTTCTGCC CCTCGACCGT CAACTCTCTG 
AACTGCCTAA AGAACTGCAT GAGCATCACG ATGGGCTCTG TGAGGCCCTC GGTGGAGCAG 
TTCCATAAGT ATCTTCCCTG GTTCCTGAAC GACCGGCCCA ACATCAAATG TCCCAAAGGC 
GGCCTGGCAG CATACAGCAC CTCTGTGAAC TTGACTTCAG ATGGCCAGGT TTTAGCCTCC 
AGGTTCATGG CCTATCACAA GCCCCTGAAA AACTCACAGG ATTACACAGA AGCTCTGCGG 
GCAGCTCGAG AGCTGGCAGC CAACATCACT GCTGACCTGC GGAAAGTGCC TGGAACAGAC 
CCGGCTTTTG AGGTCTTCCC CTACACGATC ACCAATGTGT TTTATGAGCA GTACCTGACC 
ATCCTCCCTG AGGGGCTCTT CATGCTCAGC CTCTGCCTTG TGCCCACCTT CGCTGTCTCC 
TGCCTCCTGC TGGGCCTGGA CCTGCGCTCC GGCCTCCTCA ACCTGCTCTC CATTGTCATG 
ATCCTCGTGG ACACTGTCGG CTTCATGGCC CTGTGGGGCA TCAGTTACAA TGCTGTGTCC 
CTCATCAACC TGGTCTCGGC GGTGGGCATG TCTGTGGAGT TTGTGTCCCA CATTACCCGC 
TCCTTTGCCA TCAGCACCAA GCCCACCTGG CTGGAGAGGG CCAAAGAGGC CACCATCTCT 
ATGGGAAGTG CGGTGTTTGC AGGTGTGGCC ATGACCAACC TGCCTGGCAT CCTTGTCCTG 
GGCCTCGCCA AGGCCCAGCT CATTCAGATC TTCTTCTTCC GCCTCAACCT CCTGATCACT 
CTGCTGGGCC TGCTGCATGG CTTGGTCTTC CTGCCCGTCA TCCTCAGCTA CGTGGGGCCT 
GACGTTAACC CGGCTCTGGC ACTGGAGCAG AAGCGGGCTG AGGAGGCGGT GGCAGCAGTC 
ATGGTGGCCT CTTGCCCAAA TCACCCCTCC CGAGTCTCCA CAGCTGACAA CATCTATGTC 
AACCACAGCT TTGAAGGTTC TATCAAAGGT GCTGGTGCCA TCAGCAACTT CTTGCCCAAC 
AATGGGCGGC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513630.1
CDS	1297..3654
Translation	MVARSTAQHLPAGHLLRPPQSGQYQSLRLLHQQPPAVFPEQPHAPAAHSQPDTDGADLPSRLEGPFSVLCQRSLEDEINRTTAEDLPIFATSYIVIFLYISLALGSYSSWSRVMVDSKATLGLGGVAVVLGAVMAAMGFFSYLGIRSSLVILQVVPFLVLSVGADNIFIFVLEYQRLPRRPGEPREVHIGRALGRVAPSMLLCSLSEAICFFLGALTPMPAVRTFALTSGLAVILDFLLQMSAFVALLSLDSKRQEASRLDVCCCVKPQELPPPGQGEGLLLGFFQKAYAPFLLHWITRGVVLLLFLALFGVSLYSMCHISVGLDQELALPKDSYLLDYFLFLNRYFEVGAPVYFVTTLGYNFSSEAGMNAICSSAGCNNFSFTQKIQYATEFPEQSYLAIPASSWVDDFIDWLTPSSCCRLYISGPNKDKFCPSTVNSLNCLKNCMSITMGSVRPSVEQFHKYLPWFLNDRPNIKCPKGGLAAYSTSVNLTSDGQVLASRFMAYHKPLKNSQDYTEALRAARELAANITADLRKVPGTDPAFEVFPYTITNVFYEQYLTILPEGLFMLSLCLVPTFAVSCLLLGLDLRSGLLNLLSIVMILVDTVGFMALWGISYNAVSLINLVSAVGMSVEFVSHITRSFAISTKPTWLERAKEATISMGSAVFAGVAMTNLPGILVLGLAKAQLIQIFFFRLNLLITLLGLLHGLVFLPVILSYVGPDVNPALALEQKRAEEAVAAVMVASCPNHPSRVSTADNIYVNHSFEGSIKGAGAISNFLPNNGRQF

Target ORF information:

RefSeq Version	XM_011515328.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515328.2

ORF Insert Sequence:

ATGGTCGCCC GAAGCACAGC GCAACATCTC CCTGCAGGAC ATCTGCTACG CCCCCCTCAA 
TCCGGACAAT ACCAGTCTCT ACGACTGCTG CATCAACAGC CTCCTGCAGT ATTTCCAGAA 
CAACCGCACG CTCCTGCTGC TCACAGCCAA CCAGACACTG ATGGGGCAGA CCTCCCAAGT 
CGACTGGAAG GACCATTTTC TGTACTGTGC CAACGCTCTC TGGAAGACGA GATCAATCGC 
ACCACAGCTG AAGACCTGCC CATCTTTGCC ACCAGCTACA TTGTCATATT CCTGTACATC 
TCTCTGGCCC TGGGCAGCTA TTCCAGCTGG AGCCGAGTGA TGGTGGACTC CAAGGCCACG 
CTGGGCCTCG GCGGGGTGGC CGTGGTCCTG GGAGCAGTCA TGGCTGCCAT GGGCTTCTTC 
TCCTACTTGG GTATCCGCTC CTCCCTGGTC ATCCTGCAAG TGGTTCCTTT CCTGGTGCTG 
TCCGTGGGGG CTGATAACAT CTTCATCTTT GTTCTCGAGT ACCAGAGGCT GCCCCGGAGG 
CCTGGGGAGC CACGAGAGGT CCACATTGGG CGAGCCCTAG GCAGGGTGGC TCCCAGCATG 
CTGTTGTGCA GCCTCTCTGA GGCCATCTGC TTCTTCCTAG GGGCCCTGAC CCCCATGCCA 
GCTGTGCGGA CCTTTGCCCT GACCTCTGGC CTTGCAGTGA TCCTTGACTT CCTCCTGCAG 
ATGTCAGCCT TTGTGGCCCT GCTCTCCCTG GACAGCAAGA GGCAGGAGGC CTCCCGGTTG 
GACGTCTGCT GCTGTGTCAA GCCCCAGGAG CTGCCCCCGC CTGGCCAGGG AGAGGGGCTC 
CTGCTTGGCT TCTTCCAAAA GGCTTATGCC CCCTTCCTGC TGCACTGGAT CACTCGAGGT 
GTTGTGCTGC TGCTGTTTCT CGCCCTGTTC GGAGTGAGCC TCTACTCCAT GTGCCACATC 
AGCGTGGGAC TGGACCAGGA GCTGGCCCTG CCCAAGGACT CGTACCTGCT TGACTATTTC 
CTCTTTCTGA ACCGCTACTT CGAGGTGGGG GCCCCGGTGT ACTTTGTTAC CACCTTGGGC 
TACAACTTCT CCAGCGAGGC TGGGATGAAT GCCATCTGCT CCAGTGCAGG CTGCAACAAC 
TTCTCCTTCA CCCAGAAGAT CCAGTATGCC ACAGAGTTCC CTGAGCAGTC TTACCTGGCC 
ATCCCTGCCT CCTCCTGGGT GGATGACTTC ATTGACTGGC TGACCCCGTC CTCCTGCTGC 
CGCCTTTATA TATCTGGCCC CAATAAGGAC AAGTTCTGCC CCTCGACCGT CAACTCTCTG 
AACTGCCTAA AGAACTGCAT GAGCATCACG ATGGGCTCTG TGAGGCCCTC GGTGGAGCAG 
TTCCATAAGT ATCTTCCCTG GTTCCTGAAC GACCGGCCCA ACATCAAATG TCCCAAAGGC 
GGCCTGGCAG CATACAGCAC CTCTGTGAAC TTGACTTCAG ATGGCCAGGT TTTAGCCTCC 
AGGTTCATGG CCTATCACAA GCCCCTGAAA AACTCACAGG ATTACACAGA AGCTCTGCGG 
GCAGCTCGAG AGCTGGCAGC CAACATCACT GCTGACCTGC GGAAAGTGCC TGGAACAGAC 
CCGGCTTTTG AGGTCTTCCC CTACACGATC ACCAATGTGT TTTATGAGCA GTACCTGACC 
ATCCTCCCTG AGGGGCTCTT CATGCTCAGC CTCTGCCTTG TGCCCACCTT CGCTGTCTCC 
TGCCTCCTGC TGGGCCTGGA CCTGCGCTCC GGCCTCCTCA ACCTGCTCTC CATTGTCATG 
ATCCTCGTGG ACACTGTCGG CTTCATGGCC CTGTGGGGCA TCAGTTACAA TGCTGTGTCC 
CTCATCAACC TGGTCTCGGC GGTGGGCATG TCTGTGGAGT TTGTGTCCCA CATTACCCGC 
TCCTTTGCCA TCAGCACCAA GCCCACCTGG CTGGAGAGGG CCAAAGAGGC CACCATCTCT 
ATGGGAAGTG CGGTGTTTGC AGGTGTGGCC ATGACCAACC TGCCTGGCAT CCTTGTCCTG 
GGCCTCGCCA AGGCCCAGCT CATTCAGATC TTCTTCTTCC GCCTCAACCT CCTGATCACT 
CTGCTGGGCC TGCTGCATGG CTTGGTCTTC CTGCCCGTCA TCCTCAGCTA CGTGGGGCCT 
GACGTTAACC CGGCTCTGGC ACTGGAGCAG AAGCGGGCTG AGGAGGCGGT GGCAGCAGTC 
ATGGTGGCCT CTTGCCCAAA TCACCCCTCC CGAGTCTCCA CAGCTGACAA CATCTATGTC 
AACCACAGCT TTGAAGGTTC TATCAAAGGT GCTGGTGCCA TCAGCAACTT CTTGCCCAAC 
AATGGGCGGC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPC1L1 ( NM_001101648.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001095118.1 Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 2, mRNA.

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CloneID	OHu24905
Clone ID Related Accession (Same CDS sequence)	NM_001101648.2 , NM_001101648.1
Accession Version	NM_001101648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	NPC1-like intracellular cholesterol transporter 1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004938.3, AF192522.1 and AY515256.1. On Jun 2, 2019 this sequence version replaced NM_001101648.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC117178.1, AY515256.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGAGGC TGCCCCGGAG GCCTGGGGAG CCACGAGAGG TCCACATTGG GCGAGCCCTA 
GGCAGGGTGG CTCCCAGCAT GCTGTTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTCCTA 
GGGGCCCTGA CCCCCATGCC AGCTGTGCGG ACCTTTGCCC TGACCTCTGG CCTTGCAGTG 
ATCCTTGACT TCCTCCTGCA GATGTCAGCC TTTGTGGCCC TGCTCTCCCT GGACAGCAAG 
AGGCAGGAGG CCTCCCGGTT GGACGTCTGC TGCTGTGTCA AGCCCCAGGA GCTGCCCCCG 
CCTGGCCAGG GAGAGGGGCT CCTGCTTGGC TTCTTCCAAA AGGCTTATGC CCCCTTCCTG 
CTGCACTGGA TCACTCGAGG TGTTGTGCTG CTGCTGTTTC TCGCCCTGTT CGGAGTGAGC 
CTCTACTCCA TGTGCCACAT CAGCGTGGGA CTGGACCAGG AGCTGGCCCT GCCCAAGGAC 
TCGTACCTGC TTGACTATTT CCTCTTTCTG AACCGCTACT TCGAGGTGGG GGCCCCGGTG 
TACTTTGTTA CCACCTTGGG CTACAACTTC TCCAGCGAGG CTGGGATGAA TGCCATCTGC 
TCCAGTGCAG GCTGCAACAA CTTCTCCTTC ACCCAGAAGA TCCAGTATGC CACAGAGTTC 
CCTGAGCAGT CTTACCTGGC CATCCCTGCC TCCTCCTGGG TGGATGACTT CATTGACTGG 
CTGACCCCGT CCTCCTGCTG CCGCCTTTAT ATATCTGGCC CCAATAAGGA CAAGTTCTGC 
CCCTCGACCG TCAACTCTCT GAACTGCCTA AAGAACTGCA TGAGCATCAC GATGGGCTCT 
GTGAGGCCCT CGGTGGAGCA GTTCCATAAG TATCTTCCCT GGTTCCTGAA CGACCGGCCC 
AACATCAAAT GTCCCAAAGG CGGCCTGGCA GCATACAGCA CCTCTGTGAA CTTGACTTCA 
GATGGCCAGG TTTTAGCCTC CAGGTTCATG GCCTATCACA AGCCCCTGAA AAACTCACAG 
GATTACACAG AAGCTCTGCG GGCAGCTCGA GAGCTGGCAG CCAACATCAC TGCTGACCTG 
CGGAAAGTGC CTGGAACAGA CCCGGCTTTT GAGGTCTTCC CCTACACGAT CACCAATGTG 
TTTTATGAGC AGTACCTGAC CATCCTCCCT GAGGGGCTCT TCATGCTCAG CCTCTGCCTT 
GTGCCCACCT TCGCTGTCTC CTGCCTCCTG CTGGGCCTGG ACCTGCGCTC CGGCCTCCTC 
AACCTGCTCT CCATTGTCAT GATCCTCGTG GACACTGTCG GCTTCATGGC CCTGTGGGGC 
ATCAGTTACA ATGCTGTGTC CCTCATCAAC CTGGTCTCGG CGGTGGGCAT GTCTGTGGAG 
TTTGTGTCCC ACATTACCCG CTCCTTTGCC ATCAGCACCA AGCCCACCTG GCTGGAGAGG 
GCCAAAGAGG CCACCATCTC TATGGGAAGT GCGGTGTTTG CAGGTGTGGC CATGACCAAC 
CTGCCTGGCA TCCTTGTCCT GGGCCTCGCC AAGGCCCAGC TCATTCAGAT CTTCTTCTTC 
CGCCTCAACC TCCTGATCAC TCTGCTGGGC CTGCTGCATG GCTTGGTCTT CCTGCCCGTC 
ATCCTCAGCT ACGTGGGGCC TGACGTTAAC CCGGCTCTGG CACTGGAGCA GAAGCGGGCT 
GAGGAGGCGG TGGCAGCAGT CATGGTGGCC TCTTGCCCAA ATCACCCCTC CCGAGTCTCC 
ACAGCTGACA ACATCTATGT CAACCACAGC TTTGAAGGTT CTATCAAAGG TGCTGGTGCC 
ATCAGCAACT TCTTGCCCAA CAATGGGCGG CAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095118.1
CDS	72..4070
Translation	MAEAGLRGWLLWALLLRLAQSEPYTTIHQPGYCAFYDECGKNPELSGSLMTLSNVSCLSNTPARKITGDHLILLQKICPRLYTGPNTQACCSAKQLVSLEASLSITKALLTRCPACSDNFVNLHCHNTCSPNQSLFINVTRVAQLGAGQLPAVVAYEAFYQHSFAEQSYDSCSRVRVPAAATLAVGTMCGVYGSALCNAQRWLNFQGDTGNGLAPLDITFHLLEPGQAVGSGIQPLNEGVARCNESQGDDVATCSCQDCAASCPAIARPQALDSTFYLGQMPGSLVLIIILCSVFAVVTILLVGFRVAPARDKSKMVDPKKGTSLSDKLSFSTHTLLGQFFQGWGTWVASWPLTILVLSVIPVVALAAGLVFTELTTDPVELWSAPNSQARSEKAFHDQHFGPFFRTNQVILTAPNRSSYRYDSLLLGPKNFSGILDLDLLLELLELQERLRHLQVWSPEAQRNISLQDICYAPLNPDNTSLYDCCINSLLQYFQNNRTLLLLTANQTLMGQTSQVDWKDHFLYCANAPLTFKDGTALALSCMADYGAPVFPFLAIGGYKGKDYSEAEALIMTFSLNNYPAGDPRLAQAKLWEEAFLEEMRAFQRRMAGMFQVTFMAERSLEDEINRTTAEDLPIFATSYIVIFLYISLALGSYSSWSRVMVDSKATLGLGGVAVVLGAVMAAMGFFSYLGIRSSLVILQVVPFLVLSVGADNIFIFVLEYQRLPRRPGEPREVHIGRALGRVAPSMLLCSLSEAICFFLGALTPMPAVRTFALTSGLAVILDFLLQMSAFVALLSLDSKRQEASRLDVCCCVKPQELPPPGQGEGLLLGFFQKAYAPFLLHWITRGVVLLLFLALFGVSLYSMCHISVGLDQELALPKDSYLLDYFLFLNRYFEVGAPVYFVTTLGYNFSSEAGMNAICSSAGCNNFSFTQKIQYATEFPEQSYLAIPASSWVDDFIDWLTPSSCCRLYISGPNKDKFCPSTVNSLNCLKNCMSITMGSVRPSVEQFHKYLPWFLNDRPNIKCPKGGLAAYSTSVNLTSDGQVLASRFMAYHKPLKNSQDYTEALRAARELAANITADLRKVPGTDPAFEVFPYTITNVFYEQYLTILPEGLFMLSLCLVPTFAVSCLLLGLDLRSGLLNLLSIVMILVDTVGFMALWGISYNAVSLINLVSAVGMSVEFVSHITRSFAISTKPTWLERAKEATISMGSAVFAGVAMTNLPGILVLGLAKAQLIQIFFFRLNLLITLLGLLHGLVFLPVILSYVGPDVNPALALEQKRAEEAVAAVMVASCPNHPSRVSTADNIYVNHSFEGSIKGAGAISNFLPNNGRQF

Target ORF information:

RefSeq Version	NM_001101648.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101648.2

ORF Insert Sequence:

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGAGGC TGCCCCGGAG GCCTGGGGAG CCACGAGAGG TCCACATTGG GCGAGCCCTA 
GGCAGGGTGG CTCCCAGCAT GCTGTTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTCCTA 
GGGGCCCTGA CCCCCATGCC AGCTGTGCGG ACCTTTGCCC TGACCTCTGG CCTTGCAGTG 
ATCCTTGACT TCCTCCTGCA GATGTCAGCC TTTGTGGCCC TGCTCTCCCT GGACAGCAAG 
AGGCAGGAGG CCTCCCGGTT GGACGTCTGC TGCTGTGTCA AGCCCCAGGA GCTGCCCCCG 
CCTGGCCAGG GAGAGGGGCT CCTGCTTGGC TTCTTCCAAA AGGCTTATGC CCCCTTCCTG 
CTGCACTGGA TCACTCGAGG TGTTGTGCTG CTGCTGTTTC TCGCCCTGTT CGGAGTGAGC 
CTCTACTCCA TGTGCCACAT CAGCGTGGGA CTGGACCAGG AGCTGGCCCT GCCCAAGGAC 
TCGTACCTGC TTGACTATTT CCTCTTTCTG AACCGCTACT TCGAGGTGGG GGCCCCGGTG 
TACTTTGTTA CCACCTTGGG CTACAACTTC TCCAGCGAGG CTGGGATGAA TGCCATCTGC 
TCCAGTGCAG GCTGCAACAA CTTCTCCTTC ACCCAGAAGA TCCAGTATGC CACAGAGTTC 
CCTGAGCAGT CTTACCTGGC CATCCCTGCC TCCTCCTGGG TGGATGACTT CATTGACTGG 
CTGACCCCGT CCTCCTGCTG CCGCCTTTAT ATATCTGGCC CCAATAAGGA CAAGTTCTGC 
CCCTCGACCG TCAACTCTCT GAACTGCCTA AAGAACTGCA TGAGCATCAC GATGGGCTCT 
GTGAGGCCCT CGGTGGAGCA GTTCCATAAG TATCTTCCCT GGTTCCTGAA CGACCGGCCC 
AACATCAAAT GTCCCAAAGG CGGCCTGGCA GCATACAGCA CCTCTGTGAA CTTGACTTCA 
GATGGCCAGG TTTTAGCCTC CAGGTTCATG GCCTATCACA AGCCCCTGAA AAACTCACAG 
GATTACACAG AAGCTCTGCG GGCAGCTCGA GAGCTGGCAG CCAACATCAC TGCTGACCTG 
CGGAAAGTGC CTGGAACAGA CCCGGCTTTT GAGGTCTTCC CCTACACGAT CACCAATGTG 
TTTTATGAGC AGTACCTGAC CATCCTCCCT GAGGGGCTCT TCATGCTCAG CCTCTGCCTT 
GTGCCCACCT TCGCTGTCTC CTGCCTCCTG CTGGGCCTGG ACCTGCGCTC CGGCCTCCTC 
AACCTGCTCT CCATTGTCAT GATCCTCGTG GACACTGTCG GCTTCATGGC CCTGTGGGGC 
ATCAGTTACA ATGCTGTGTC CCTCATCAAC CTGGTCTCGG CGGTGGGCAT GTCTGTGGAG 
TTTGTGTCCC ACATTACCCG CTCCTTTGCC ATCAGCACCA AGCCCACCTG GCTGGAGAGG 
GCCAAAGAGG CCACCATCTC TATGGGAAGT GCGGTGTTTG CAGGTGTGGC CATGACCAAC 
CTGCCTGGCA TCCTTGTCCT GGGCCTCGCC AAGGCCCAGC TCATTCAGAT CTTCTTCTTC 
CGCCTCAACC TCCTGATCAC TCTGCTGGGC CTGCTGCATG GCTTGGTCTT CCTGCCCGTC 
ATCCTCAGCT ACGTGGGGCC TGACGTTAAC CCGGCTCTGG CACTGGAGCA GAAGCGGGCT 
GAGGAGGCGG TGGCAGCAGT CATGGTGGCC TCTTGCCCAA ATCACCCCTC CCGAGTCTCC 
ACAGCTGACA ACATCTATGT CAACCACAGC TTTGAAGGTT CTATCAAAGG TGCTGGTGCC 
ATCAGCAACT TCTTGCCCAA CAATGGGCGG CAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPC1L1 ( NM_001101648.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001095118.1 Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu24905
Clone ID Related Accession (Same CDS sequence)	NM_001101648.2 , NM_001101648.1
Accession Version	NM_001101648.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	NPC1-like intracellular cholesterol transporter 1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF192522.1 and AY515256.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC117178.1, AY437865.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGAGGC TGCCCCGGAG GCCTGGGGAG CCACGAGAGG TCCACATTGG GCGAGCCCTA 
GGCAGGGTGG CTCCCAGCAT GCTGTTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTCCTA 
GGGGCCCTGA CCCCCATGCC AGCTGTGCGG ACCTTTGCCC TGACCTCTGG CCTTGCAGTG 
ATCCTTGACT TCCTCCTGCA GATGTCAGCC TTTGTGGCCC TGCTCTCCCT GGACAGCAAG 
AGGCAGGAGG CCTCCCGGTT GGACGTCTGC TGCTGTGTCA AGCCCCAGGA GCTGCCCCCG 
CCTGGCCAGG GAGAGGGGCT CCTGCTTGGC TTCTTCCAAA AGGCTTATGC CCCCTTCCTG 
CTGCACTGGA TCACTCGAGG TGTTGTGCTG CTGCTGTTTC TCGCCCTGTT CGGAGTGAGC 
CTCTACTCCA TGTGCCACAT CAGCGTGGGA CTGGACCAGG AGCTGGCCCT GCCCAAGGAC 
TCGTACCTGC TTGACTATTT CCTCTTTCTG AACCGCTACT TCGAGGTGGG GGCCCCGGTG 
TACTTTGTTA CCACCTTGGG CTACAACTTC TCCAGCGAGG CTGGGATGAA TGCCATCTGC 
TCCAGTGCAG GCTGCAACAA CTTCTCCTTC ACCCAGAAGA TCCAGTATGC CACAGAGTTC 
CCTGAGCAGT CTTACCTGGC CATCCCTGCC TCCTCCTGGG TGGATGACTT CATTGACTGG 
CTGACCCCGT CCTCCTGCTG CCGCCTTTAT ATATCTGGCC CCAATAAGGA CAAGTTCTGC 
CCCTCGACCG TCAACTCTCT GAACTGCCTA AAGAACTGCA TGAGCATCAC GATGGGCTCT 
GTGAGGCCCT CGGTGGAGCA GTTCCATAAG TATCTTCCCT GGTTCCTGAA CGACCGGCCC 
AACATCAAAT GTCCCAAAGG CGGCCTGGCA GCATACAGCA CCTCTGTGAA CTTGACTTCA 
GATGGCCAGG TTTTAGCCTC CAGGTTCATG GCCTATCACA AGCCCCTGAA AAACTCACAG 
GATTACACAG AAGCTCTGCG GGCAGCTCGA GAGCTGGCAG CCAACATCAC TGCTGACCTG 
CGGAAAGTGC CTGGAACAGA CCCGGCTTTT GAGGTCTTCC CCTACACGAT CACCAATGTG 
TTTTATGAGC AGTACCTGAC CATCCTCCCT GAGGGGCTCT TCATGCTCAG CCTCTGCCTT 
GTGCCCACCT TCGCTGTCTC CTGCCTCCTG CTGGGCCTGG ACCTGCGCTC CGGCCTCCTC 
AACCTGCTCT CCATTGTCAT GATCCTCGTG GACACTGTCG GCTTCATGGC CCTGTGGGGC 
ATCAGTTACA ATGCTGTGTC CCTCATCAAC CTGGTCTCGG CGGTGGGCAT GTCTGTGGAG 
TTTGTGTCCC ACATTACCCG CTCCTTTGCC ATCAGCACCA AGCCCACCTG GCTGGAGAGG 
GCCAAAGAGG CCACCATCTC TATGGGAAGT GCGGTGTTTG CAGGTGTGGC CATGACCAAC 
CTGCCTGGCA TCCTTGTCCT GGGCCTCGCC AAGGCCCAGC TCATTCAGAT CTTCTTCTTC 
CGCCTCAACC TCCTGATCAC TCTGCTGGGC CTGCTGCATG GCTTGGTCTT CCTGCCCGTC 
ATCCTCAGCT ACGTGGGGCC TGACGTTAAC CCGGCTCTGG CACTGGAGCA GAAGCGGGCT 
GAGGAGGCGG TGGCAGCAGT CATGGTGGCC TCTTGCCCAA ATCACCCCTC CCGAGTCTCC 
ACAGCTGACA ACATCTATGT CAACCACAGC TTTGAAGGTT CTATCAAAGG TGCTGGTGCC 
ATCAGCAACT TCTTGCCCAA CAATGGGCGG CAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095118.1
CDS	57..4055
Translation	MAEAGLRGWLLWALLLRLAQSEPYTTIHQPGYCAFYDECGKNPELSGSLMTLSNVSCLSNTPARKITGDHLILLQKICPRLYTGPNTQACCSAKQLVSLEASLSITKALLTRCPACSDNFVNLHCHNTCSPNQSLFINVTRVAQLGAGQLPAVVAYEAFYQHSFAEQSYDSCSRVRVPAAATLAVGTMCGVYGSALCNAQRWLNFQGDTGNGLAPLDITFHLLEPGQAVGSGIQPLNEGVARCNESQGDDVATCSCQDCAASCPAIARPQALDSTFYLGQMPGSLVLIIILCSVFAVVTILLVGFRVAPARDKSKMVDPKKGTSLSDKLSFSTHTLLGQFFQGWGTWVASWPLTILVLSVIPVVALAAGLVFTELTTDPVELWSAPNSQARSEKAFHDQHFGPFFRTNQVILTAPNRSSYRYDSLLLGPKNFSGILDLDLLLELLELQERLRHLQVWSPEAQRNISLQDICYAPLNPDNTSLYDCCINSLLQYFQNNRTLLLLTANQTLMGQTSQVDWKDHFLYCANAPLTFKDGTALALSCMADYGAPVFPFLAIGGYKGKDYSEAEALIMTFSLNNYPAGDPRLAQAKLWEEAFLEEMRAFQRRMAGMFQVTFMAERSLEDEINRTTAEDLPIFATSYIVIFLYISLALGSYSSWSRVMVDSKATLGLGGVAVVLGAVMAAMGFFSYLGIRSSLVILQVVPFLVLSVGADNIFIFVLEYQRLPRRPGEPREVHIGRALGRVAPSMLLCSLSEAICFFLGALTPMPAVRTFALTSGLAVILDFLLQMSAFVALLSLDSKRQEASRLDVCCCVKPQELPPPGQGEGLLLGFFQKAYAPFLLHWITRGVVLLLFLALFGVSLYSMCHISVGLDQELALPKDSYLLDYFLFLNRYFEVGAPVYFVTTLGYNFSSEAGMNAICSSAGCNNFSFTQKIQYATEFPEQSYLAIPASSWVDDFIDWLTPSSCCRLYISGPNKDKFCPSTVNSLNCLKNCMSITMGSVRPSVEQFHKYLPWFLNDRPNIKCPKGGLAAYSTSVNLTSDGQVLASRFMAYHKPLKNSQDYTEALRAARELAANITADLRKVPGTDPAFEVFPYTITNVFYEQYLTILPEGLFMLSLCLVPTFAVSCLLLGLDLRSGLLNLLSIVMILVDTVGFMALWGISYNAVSLINLVSAVGMSVEFVSHITRSFAISTKPTWLERAKEATISMGSAVFAGVAMTNLPGILVLGLAKAQLIQIFFFRLNLLITLLGLLHGLVFLPVILSYVGPDVNPALALEQKRAEEAVAAVMVASCPNHPSRVSTADNIYVNHSFEGSIKGAGAISNFLPNNGRQF

Target ORF information:

RefSeq Version	NM_001101648.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101648.1

ORF Insert Sequence:

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGAGGC TGCCCCGGAG GCCTGGGGAG CCACGAGAGG TCCACATTGG GCGAGCCCTA 
GGCAGGGTGG CTCCCAGCAT GCTGTTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTCCTA 
GGGGCCCTGA CCCCCATGCC AGCTGTGCGG ACCTTTGCCC TGACCTCTGG CCTTGCAGTG 
ATCCTTGACT TCCTCCTGCA GATGTCAGCC TTTGTGGCCC TGCTCTCCCT GGACAGCAAG 
AGGCAGGAGG CCTCCCGGTT GGACGTCTGC TGCTGTGTCA AGCCCCAGGA GCTGCCCCCG 
CCTGGCCAGG GAGAGGGGCT CCTGCTTGGC TTCTTCCAAA AGGCTTATGC CCCCTTCCTG 
CTGCACTGGA TCACTCGAGG TGTTGTGCTG CTGCTGTTTC TCGCCCTGTT CGGAGTGAGC 
CTCTACTCCA TGTGCCACAT CAGCGTGGGA CTGGACCAGG AGCTGGCCCT GCCCAAGGAC 
TCGTACCTGC TTGACTATTT CCTCTTTCTG AACCGCTACT TCGAGGTGGG GGCCCCGGTG 
TACTTTGTTA CCACCTTGGG CTACAACTTC TCCAGCGAGG CTGGGATGAA TGCCATCTGC 
TCCAGTGCAG GCTGCAACAA CTTCTCCTTC ACCCAGAAGA TCCAGTATGC CACAGAGTTC 
CCTGAGCAGT CTTACCTGGC CATCCCTGCC TCCTCCTGGG TGGATGACTT CATTGACTGG 
CTGACCCCGT CCTCCTGCTG CCGCCTTTAT ATATCTGGCC CCAATAAGGA CAAGTTCTGC 
CCCTCGACCG TCAACTCTCT GAACTGCCTA AAGAACTGCA TGAGCATCAC GATGGGCTCT 
GTGAGGCCCT CGGTGGAGCA GTTCCATAAG TATCTTCCCT GGTTCCTGAA CGACCGGCCC 
AACATCAAAT GTCCCAAAGG CGGCCTGGCA GCATACAGCA CCTCTGTGAA CTTGACTTCA 
GATGGCCAGG TTTTAGCCTC CAGGTTCATG GCCTATCACA AGCCCCTGAA AAACTCACAG 
GATTACACAG AAGCTCTGCG GGCAGCTCGA GAGCTGGCAG CCAACATCAC TGCTGACCTG 
CGGAAAGTGC CTGGAACAGA CCCGGCTTTT GAGGTCTTCC CCTACACGAT CACCAATGTG 
TTTTATGAGC AGTACCTGAC CATCCTCCCT GAGGGGCTCT TCATGCTCAG CCTCTGCCTT 
GTGCCCACCT TCGCTGTCTC CTGCCTCCTG CTGGGCCTGG ACCTGCGCTC CGGCCTCCTC 
AACCTGCTCT CCATTGTCAT GATCCTCGTG GACACTGTCG GCTTCATGGC CCTGTGGGGC 
ATCAGTTACA ATGCTGTGTC CCTCATCAAC CTGGTCTCGG CGGTGGGCAT GTCTGTGGAG 
TTTGTGTCCC ACATTACCCG CTCCTTTGCC ATCAGCACCA AGCCCACCTG GCTGGAGAGG 
GCCAAAGAGG CCACCATCTC TATGGGAAGT GCGGTGTTTG CAGGTGTGGC CATGACCAAC 
CTGCCTGGCA TCCTTGTCCT GGGCCTCGCC AAGGCCCAGC TCATTCAGAT CTTCTTCTTC 
CGCCTCAACC TCCTGATCAC TCTGCTGGGC CTGCTGCATG GCTTGGTCTT CCTGCCCGTC 
ATCCTCAGCT ACGTGGGGCC TGACGTTAAC CCGGCTCTGG CACTGGAGCA GAAGCGGGCT 
GAGGAGGCGG TGGCAGCAGT CATGGTGGCC TCTTGCCCAA ATCACCCCTC CCGAGTCTCC 
ACAGCTGACA ACATCTATGT CAACCACAGC TTTGAAGGTT CTATCAAAGG TGCTGGTGCC 
ATCAGCAACT TCTTGCCCAA CAATGGGCGG CAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPC1L1 ( XM_011515326.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513628.1 Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant X1, mRNA.

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CloneID	OHu67389
Clone ID Related Accession (Same CDS sequence)	XM_011515326.2 , XM_011515326.3
Accession Version	XM_011515326.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	Niemann-Pick C1-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515326.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGATGT CAGCCTTTGT GGCCCTGCTC TCCCTGGACA GCAAGAGGCA GGAGGCCTCC 
CGGTTGGACG TCTGCTGCTG TGTCAAGCCC CAGGAGCTGC CCCCGCCTGG CCAGGGAGAG 
GGGCTCCTGC TTGGCTTCTT CCAAAAGGCT TATGCCCCCT TCCTGCTGCA CTGGATCACT 
CGAGGTGTTG TGCTGCTGCT GTTTCTCGCC CTGTTCGGAG TGAGCCTCTA CTCCATGTGC 
CACATCAGCG TGGGACTGGA CCAGGAGCTG GCCCTGCCCA AGGACTCGTA CCTGCTTGAC 
TATTTCCTCT TTCTGAACCG CTACTTCGAG GTGGGGGCCC CGGTGTACTT TGTTACCACC 
TTGGGCTACA ACTTCTCCAG CGAGGCTGGG ATGAATGCCA TCTGCTCCAG TGCAGGCTGC 
AACAACTTCT CCTTCACCCA GAAGATCCAG TATGCCACAG AGTTCCCTGA GCAGTCTTAC 
CTGGCCATCC CTGCCTCCTC CTGGGTGGAT GACTTCATTG ACTGGCTGAC CCCGTCCTCC 
TGCTGCCGCC TTTATATATC TGGCCCCAAT AAGGACAAGT TCTGCCCCTC GACCGTCAAC 
TCTCTGAACT GCCTAAAGAA CTGCATGAGC ATCACGATGG GCTCTGTGAG GCCCTCGGTG 
GAGCAGTTCC ATAAGTATCT TCCCTGGTTC CTGAACGACC GGCCCAACAT CAAATGTCCC 
AAAGGCGGCC TGGCAGCATA CAGCACCTCT GTGAACTTGA CTTCAGATGG CCAGGTTTTA 
GCCTCCAGGT TCATGGCCTA TCACAAGCCC CTGAAAAACT CACAGGATTA CACAGAAGCT 
CTGCGGGCAG CTCGAGAGCT GGCAGCCAAC ATCACTGCTG ACCTGCGGAA AGTGCCTGGA 
ACAGACCCGG CTTTTGAGGT CTTCCCCTAC ACGATCACCA ATGTGTTTTA TGAGCAGTAC 
CTGACCATCC TCCCTGAGGG GCTCTTCATG CTCAGCCTCT GCCTTGTGCC CACCTTCGCT 
GTCTCCTGCC TCCTGCTGGG CCTGGACCTG CGCTCCGGCC TCCTCAACCT GCTCTCCATT 
GTCATGATCC TCGTGGACAC TGTCGGCTTC ATGGCCCTGT GGGGCATCAG TTACAATGCT 
GTGTCCCTCA TCAACCTGGT CTCGGCGGTG GGCATGTCTG TGGAGTTTGT GTCCCACATT 
ACCCGCTCCT TTGCCATCAG CACCAAGCCC ACCTGGCTGG AGAGGGCCAA AGAGGCCACC 
ATCTCTATGG GAAGTGCGGT GTTTGCAGGT GTGGCCATGA CCAACCTGCC TGGCATCCTT 
GTCCTGGGCC TCGCCAAGGC CCAGCTCATT CAGATCTTCT TCTTCCGCCT CAACCTCCTG 
ATCACTCTGC TGGGCCTGCT GCATGGCTTG GTCTTCCTGC CCGTCATCCT CAGCTACGTG 
GGGCCTGACG TTAACCCGGC TCTGGCACTG GAGCAGAAGC GGGCTGAGGA GGCGGTGGCA 
GCAGTCATGG TGGCCTCTTG CCCAAATCAC CCCTCCCGAG TCTCCACAGC TGACAACATC 
TATGTCAACC ACAGCTTTGA AGGTTCTATC AAAGGTGCTG GTGCCATCAG CAACTTCTTG 
CCCAACAATG GGCGGCAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513628.1
CDS	103..3906
Translation	MAEAGLRGWLLWALLLRLAQSEPYTTIHQPGYCAFYDECGKNPELSGSLMTLSNVSCLSNTPARKITGDHLILLQKICPRLYTGPNTQACCSAKQLVSLEASLSITKALLTRCPACSDNFVNLHCHNTCSPNQSLFINVTRVAQLGAGQLPAVVAYEAFYQHSFAEQSYDSCSRVRVPAAATLAVGTMCGVYGSALCNAQRWLNFQGDTGNGLAPLDITFHLLEPGQAVGSGIQPLNEGVARCNESQGDDVATCSCQDCAASCPAIARPQALDSTFYLGQMPGSLVLIIILCSVFAVVTILLVGFRVAPARDKSKMVDPKKGTSLSDKLSFSTHTLLGQFFQGWGTWVASWPLTILVLSVIPVVALAAGLVFTELTTDPVELWSAPNSQARSEKAFHDQHFGPFFRTNQVILTAPNRSSYRYDSLLLGPKNFSGILDLDLLLELLELQERLRHLQVWSPEAQRNISLQDICYAPLNPDNTSLYDCCINSLLQYFQNNRTLLLLTANQTLMGQTSQVDWKDHFLYCANAPLTFKDGTALALSCMADYGAPVFPFLAIGGYKGKDYSEAEALIMTFSLNNYPAGDPRLAQAKLWEEAFLEEMRAFQRRMAGMFQVTFMAERSLEDEINRTTAEDLPIFATSYIVIFLYISLALGSYSSWSRVMVDSKATLGLGGVAVVLGAVMAAMGFFSYLGIRSSLVILQVVPFLVLSVGADNIFIFVLEYQMSAFVALLSLDSKRQEASRLDVCCCVKPQELPPPGQGEGLLLGFFQKAYAPFLLHWITRGVVLLLFLALFGVSLYSMCHISVGLDQELALPKDSYLLDYFLFLNRYFEVGAPVYFVTTLGYNFSSEAGMNAICSSAGCNNFSFTQKIQYATEFPEQSYLAIPASSWVDDFIDWLTPSSCCRLYISGPNKDKFCPSTVNSLNCLKNCMSITMGSVRPSVEQFHKYLPWFLNDRPNIKCPKGGLAAYSTSVNLTSDGQVLASRFMAYHKPLKNSQDYTEALRAARELAANITADLRKVPGTDPAFEVFPYTITNVFYEQYLTILPEGLFMLSLCLVPTFAVSCLLLGLDLRSGLLNLLSIVMILVDTVGFMALWGISYNAVSLINLVSAVGMSVEFVSHITRSFAISTKPTWLERAKEATISMGSAVFAGVAMTNLPGILVLGLAKAQLIQIFFFRLNLLITLLGLLHGLVFLPVILSYVGPDVNPALALEQKRAEEAVAAVMVASCPNHPSRVSTADNIYVNHSFEGSIKGAGAISNFLPNNGRQF

Target ORF information:

RefSeq Version	XM_011515326.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515326.2

ORF Insert Sequence:

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGATGT CAGCCTTTGT GGCCCTGCTC TCCCTGGACA GCAAGAGGCA GGAGGCCTCC 
CGGTTGGACG TCTGCTGCTG TGTCAAGCCC CAGGAGCTGC CCCCGCCTGG CCAGGGAGAG 
GGGCTCCTGC TTGGCTTCTT CCAAAAGGCT TATGCCCCCT TCCTGCTGCA CTGGATCACT 
CGAGGTGTTG TGCTGCTGCT GTTTCTCGCC CTGTTCGGAG TGAGCCTCTA CTCCATGTGC 
CACATCAGCG TGGGACTGGA CCAGGAGCTG GCCCTGCCCA AGGACTCGTA CCTGCTTGAC 
TATTTCCTCT TTCTGAACCG CTACTTCGAG GTGGGGGCCC CGGTGTACTT TGTTACCACC 
TTGGGCTACA ACTTCTCCAG CGAGGCTGGG ATGAATGCCA TCTGCTCCAG TGCAGGCTGC 
AACAACTTCT CCTTCACCCA GAAGATCCAG TATGCCACAG AGTTCCCTGA GCAGTCTTAC 
CTGGCCATCC CTGCCTCCTC CTGGGTGGAT GACTTCATTG ACTGGCTGAC CCCGTCCTCC 
TGCTGCCGCC TTTATATATC TGGCCCCAAT AAGGACAAGT TCTGCCCCTC GACCGTCAAC 
TCTCTGAACT GCCTAAAGAA CTGCATGAGC ATCACGATGG GCTCTGTGAG GCCCTCGGTG 
GAGCAGTTCC ATAAGTATCT TCCCTGGTTC CTGAACGACC GGCCCAACAT CAAATGTCCC 
AAAGGCGGCC TGGCAGCATA CAGCACCTCT GTGAACTTGA CTTCAGATGG CCAGGTTTTA 
GCCTCCAGGT TCATGGCCTA TCACAAGCCC CTGAAAAACT CACAGGATTA CACAGAAGCT 
CTGCGGGCAG CTCGAGAGCT GGCAGCCAAC ATCACTGCTG ACCTGCGGAA AGTGCCTGGA 
ACAGACCCGG CTTTTGAGGT CTTCCCCTAC ACGATCACCA ATGTGTTTTA TGAGCAGTAC 
CTGACCATCC TCCCTGAGGG GCTCTTCATG CTCAGCCTCT GCCTTGTGCC CACCTTCGCT 
GTCTCCTGCC TCCTGCTGGG CCTGGACCTG CGCTCCGGCC TCCTCAACCT GCTCTCCATT 
GTCATGATCC TCGTGGACAC TGTCGGCTTC ATGGCCCTGT GGGGCATCAG TTACAATGCT 
GTGTCCCTCA TCAACCTGGT CTCGGCGGTG GGCATGTCTG TGGAGTTTGT GTCCCACATT 
ACCCGCTCCT TTGCCATCAG CACCAAGCCC ACCTGGCTGG AGAGGGCCAA AGAGGCCACC 
ATCTCTATGG GAAGTGCGGT GTTTGCAGGT GTGGCCATGA CCAACCTGCC TGGCATCCTT 
GTCCTGGGCC TCGCCAAGGC CCAGCTCATT CAGATCTTCT TCTTCCGCCT CAACCTCCTG 
ATCACTCTGC TGGGCCTGCT GCATGGCTTG GTCTTCCTGC CCGTCATCCT CAGCTACGTG 
GGGCCTGACG TTAACCCGGC TCTGGCACTG GAGCAGAAGC GGGCTGAGGA GGCGGTGGCA 
GCAGTCATGG TGGCCTCTTG CCCAAATCAC CCCTCCCGAG TCTCCACAGC TGACAACATC 
TATGTCAACC ACAGCTTTGA AGGTTCTATC AAAGGTGCTG GTGCCATCAG CAACTTCTTG 
CCCAACAATG GGCGGCAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPC1L1 ( XM_011515326.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513628.1 Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant X1, mRNA.

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CloneID	OHu67389
Clone ID Related Accession (Same CDS sequence)	XM_011515326.2 , XM_011515326.3
Accession Version	XM_011515326.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NPC1-like intracellular cholesterol transporter 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011515326.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGATGT CAGCCTTTGT GGCCCTGCTC TCCCTGGACA GCAAGAGGCA GGAGGCCTCC 
CGGTTGGACG TCTGCTGCTG TGTCAAGCCC CAGGAGCTGC CCCCGCCTGG CCAGGGAGAG 
GGGCTCCTGC TTGGCTTCTT CCAAAAGGCT TATGCCCCCT TCCTGCTGCA CTGGATCACT 
CGAGGTGTTG TGCTGCTGCT GTTTCTCGCC CTGTTCGGAG TGAGCCTCTA CTCCATGTGC 
CACATCAGCG TGGGACTGGA CCAGGAGCTG GCCCTGCCCA AGGACTCGTA CCTGCTTGAC 
TATTTCCTCT TTCTGAACCG CTACTTCGAG GTGGGGGCCC CGGTGTACTT TGTTACCACC 
TTGGGCTACA ACTTCTCCAG CGAGGCTGGG ATGAATGCCA TCTGCTCCAG TGCAGGCTGC 
AACAACTTCT CCTTCACCCA GAAGATCCAG TATGCCACAG AGTTCCCTGA GCAGTCTTAC 
CTGGCCATCC CTGCCTCCTC CTGGGTGGAT GACTTCATTG ACTGGCTGAC CCCGTCCTCC 
TGCTGCCGCC TTTATATATC TGGCCCCAAT AAGGACAAGT TCTGCCCCTC GACCGTCAAC 
TCTCTGAACT GCCTAAAGAA CTGCATGAGC ATCACGATGG GCTCTGTGAG GCCCTCGGTG 
GAGCAGTTCC ATAAGTATCT TCCCTGGTTC CTGAACGACC GGCCCAACAT CAAATGTCCC 
AAAGGCGGCC TGGCAGCATA CAGCACCTCT GTGAACTTGA CTTCAGATGG CCAGGTTTTA 
GCCTCCAGGT TCATGGCCTA TCACAAGCCC CTGAAAAACT CACAGGATTA CACAGAAGCT 
CTGCGGGCAG CTCGAGAGCT GGCAGCCAAC ATCACTGCTG ACCTGCGGAA AGTGCCTGGA 
ACAGACCCGG CTTTTGAGGT CTTCCCCTAC ACGATCACCA ATGTGTTTTA TGAGCAGTAC 
CTGACCATCC TCCCTGAGGG GCTCTTCATG CTCAGCCTCT GCCTTGTGCC CACCTTCGCT 
GTCTCCTGCC TCCTGCTGGG CCTGGACCTG CGCTCCGGCC TCCTCAACCT GCTCTCCATT 
GTCATGATCC TCGTGGACAC TGTCGGCTTC ATGGCCCTGT GGGGCATCAG TTACAATGCT 
GTGTCCCTCA TCAACCTGGT CTCGGCGGTG GGCATGTCTG TGGAGTTTGT GTCCCACATT 
ACCCGCTCCT TTGCCATCAG CACCAAGCCC ACCTGGCTGG AGAGGGCCAA AGAGGCCACC 
ATCTCTATGG GAAGTGCGGT GTTTGCAGGT GTGGCCATGA CCAACCTGCC TGGCATCCTT 
GTCCTGGGCC TCGCCAAGGC CCAGCTCATT CAGATCTTCT TCTTCCGCCT CAACCTCCTG 
ATCACTCTGC TGGGCCTGCT GCATGGCTTG GTCTTCCTGC CCGTCATCCT CAGCTACGTG 
GGGCCTGACG TTAACCCGGC TCTGGCACTG GAGCAGAAGC GGGCTGAGGA GGCGGTGGCA 
GCAGTCATGG TGGCCTCTTG CCCAAATCAC CCCTCCCGAG TCTCCACAGC TGACAACATC 
TATGTCAACC ACAGCTTTGA AGGTTCTATC AAAGGTGCTG GTGCCATCAG CAACTTCTTG 
CCCAACAATG GGCGGCAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513628.1
CDS	106..3909
Translation	MAEAGLRGWLLWALLLRLAQSEPYTTIHQPGYCAFYDECGKNPELSGSLMTLSNVSCLSNTPARKITGDHLILLQKICPRLYTGPNTQACCSAKQLVSLEASLSITKALLTRCPACSDNFVNLHCHNTCSPNQSLFINVTRVAQLGAGQLPAVVAYEAFYQHSFAEQSYDSCSRVRVPAAATLAVGTMCGVYGSALCNAQRWLNFQGDTGNGLAPLDITFHLLEPGQAVGSGIQPLNEGVARCNESQGDDVATCSCQDCAASCPAIARPQALDSTFYLGQMPGSLVLIIILCSVFAVVTILLVGFRVAPARDKSKMVDPKKGTSLSDKLSFSTHTLLGQFFQGWGTWVASWPLTILVLSVIPVVALAAGLVFTELTTDPVELWSAPNSQARSEKAFHDQHFGPFFRTNQVILTAPNRSSYRYDSLLLGPKNFSGILDLDLLLELLELQERLRHLQVWSPEAQRNISLQDICYAPLNPDNTSLYDCCINSLLQYFQNNRTLLLLTANQTLMGQTSQVDWKDHFLYCANAPLTFKDGTALALSCMADYGAPVFPFLAIGGYKGKDYSEAEALIMTFSLNNYPAGDPRLAQAKLWEEAFLEEMRAFQRRMAGMFQVTFMAERSLEDEINRTTAEDLPIFATSYIVIFLYISLALGSYSSWSRVMVDSKATLGLGGVAVVLGAVMAAMGFFSYLGIRSSLVILQVVPFLVLSVGADNIFIFVLEYQMSAFVALLSLDSKRQEASRLDVCCCVKPQELPPPGQGEGLLLGFFQKAYAPFLLHWITRGVVLLLFLALFGVSLYSMCHISVGLDQELALPKDSYLLDYFLFLNRYFEVGAPVYFVTTLGYNFSSEAGMNAICSSAGCNNFSFTQKIQYATEFPEQSYLAIPASSWVDDFIDWLTPSSCCRLYISGPNKDKFCPSTVNSLNCLKNCMSITMGSVRPSVEQFHKYLPWFLNDRPNIKCPKGGLAAYSTSVNLTSDGQVLASRFMAYHKPLKNSQDYTEALRAARELAANITADLRKVPGTDPAFEVFPYTITNVFYEQYLTILPEGLFMLSLCLVPTFAVSCLLLGLDLRSGLLNLLSIVMILVDTVGFMALWGISYNAVSLINLVSAVGMSVEFVSHITRSFAISTKPTWLERAKEATISMGSAVFAGVAMTNLPGILVLGLAKAQLIQIFFFRLNLLITLLGLLHGLVFLPVILSYVGPDVNPALALEQKRAEEAVAAVMVASCPNHPSRVSTADNIYVNHSFEGSIKGAGAISNFLPNNGRQF

Target ORF information:

RefSeq Version	XM_011515326.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NPC1 like intracellular cholesterol transporter 1 (NPC1L1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515326.3

ORF Insert Sequence:

ATGGCGGAGG CCGGCCTGAG GGGCTGGCTG CTGTGGGCCC TGCTCCTGCG CTTGGCCCAG 
AGTGAGCCTT ACACAACCAT CCACCAGCCT GGCTACTGCG CCTTCTATGA CGAATGTGGG 
AAGAACCCAG AGCTGTCTGG AAGCCTCATG ACACTCTCCA ACGTGTCCTG CCTGTCCAAC 
ACGCCGGCCC GCAAGATCAC AGGTGATCAC CTGATCCTAT TACAGAAGAT CTGCCCCCGC 
CTCTACACCG GCCCCAACAC CCAAGCCTGC TGCTCCGCCA AGCAGCTGGT ATCACTGGAA 
GCGAGTCTGT CGATCACCAA GGCCCTCCTC ACCCGCTGCC CAGCCTGCTC TGACAATTTT 
GTGAACCTGC ACTGCCACAA CACGTGCAGC CCCAATCAGA GCCTCTTCAT CAATGTGACC 
CGCGTGGCCC AGCTAGGGGC TGGACAACTC CCAGCTGTGG TGGCCTATGA GGCCTTCTAC 
CAGCATAGCT TTGCCGAGCA GAGCTATGAC TCCTGCAGCC GTGTGCGCGT CCCTGCAGCT 
GCCACGCTGG CTGTGGGCAC CATGTGTGGC GTGTATGGCT CTGCCCTTTG CAATGCCCAG 
CGCTGGCTCA ACTTCCAGGG AGACACAGGC AATGGTCTGG CCCCACTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCGTGGGG AGTGGGATTC AGCCTCTGAA TGAGGGGGTT 
GCACGTTGCA ATGAGTCCCA AGGTGACGAC GTGGCGACCT GCTCCTGCCA AGACTGTGCT 
GCATCCTGTC CTGCCATAGC CCGCCCCCAG GCCCTCGACT CCACCTTCTA CCTGGGCCAG 
ATGCCGGGCA GTCTGGTCCT CATCATCATC CTCTGCTCTG TCTTCGCTGT GGTCACCATC 
CTGCTTGTGG GATTCCGTGT GGCCCCCGCC AGGGACAAAA GCAAGATGGT GGACCCCAAG 
AAGGGCACCA GCCTCTCTGA CAAGCTCAGC TTCTCCACCC ACACCCTCCT TGGCCAGTTC 
TTCCAGGGCT GGGGCACGTG GGTGGCTTCG TGGCCTCTGA CCATCTTGGT GCTATCTGTC 
ATCCCGGTGG TGGCCTTGGC AGCGGGCCTG GTCTTTACAG AACTCACTAC GGACCCCGTG 
GAGCTGTGGT CGGCCCCCAA CAGCCAAGCC CGGAGTGAGA AAGCTTTCCA TGACCAGCAT 
TTCGGCCCCT TCTTCCGAAC CAACCAGGTG ATCCTGACGG CTCCTAACCG GTCCAGCTAC 
AGGTATGACT CTCTGCTGCT GGGGCCCAAG AACTTCAGCG GAATCCTGGA CCTGGACTTG 
CTGCTGGAGC TGCTAGAGCT GCAGGAGAGG CTGCGGCACC TCCAGGTATG GTCGCCCGAA 
GCACAGCGCA ACATCTCCCT GCAGGACATC TGCTACGCCC CCCTCAATCC GGACAATACC 
AGTCTCTACG ACTGCTGCAT CAACAGCCTC CTGCAGTATT TCCAGAACAA CCGCACGCTC 
CTGCTGCTCA CAGCCAACCA GACACTGATG GGGCAGACCT CCCAAGTCGA CTGGAAGGAC 
CATTTTCTGT ACTGTGCCAA TGCCCCGCTC ACCTTCAAGG ATGGCACAGC CCTGGCCCTG 
AGCTGCATGG CTGACTACGG GGCCCCTGTC TTCCCCTTCC TTGCCATTGG GGGGTACAAA 
GGAAAGGACT ATTCTGAGGC AGAGGCCCTG ATCATGACGT TCTCCCTCAA CAATTACCCT 
GCCGGGGACC CCCGTCTGGC CCAGGCCAAG CTGTGGGAGG AGGCCTTCTT AGAGGAAATG 
CGAGCCTTCC AGCGTCGGAT GGCTGGCATG TTCCAGGTCA CGTTCATGGC TGAGCGCTCT 
CTGGAAGACG AGATCAATCG CACCACAGCT GAAGACCTGC CCATCTTTGC CACCAGCTAC 
ATTGTCATAT TCCTGTACAT CTCTCTGGCC CTGGGCAGCT ATTCCAGCTG GAGCCGAGTG 
ATGGTGGACT CCAAGGCCAC GCTGGGCCTC GGCGGGGTGG CCGTGGTCCT GGGAGCAGTC 
ATGGCTGCCA TGGGCTTCTT CTCCTACTTG GGTATCCGCT CCTCCCTGGT CATCCTGCAA 
GTGGTTCCTT TCCTGGTGCT GTCCGTGGGG GCTGATAACA TCTTCATCTT TGTTCTCGAG 
TACCAGATGT CAGCCTTTGT GGCCCTGCTC TCCCTGGACA GCAAGAGGCA GGAGGCCTCC 
CGGTTGGACG TCTGCTGCTG TGTCAAGCCC CAGGAGCTGC CCCCGCCTGG CCAGGGAGAG 
GGGCTCCTGC TTGGCTTCTT CCAAAAGGCT TATGCCCCCT TCCTGCTGCA CTGGATCACT 
CGAGGTGTTG TGCTGCTGCT GTTTCTCGCC CTGTTCGGAG TGAGCCTCTA CTCCATGTGC 
CACATCAGCG TGGGACTGGA CCAGGAGCTG GCCCTGCCCA AGGACTCGTA CCTGCTTGAC 
TATTTCCTCT TTCTGAACCG CTACTTCGAG GTGGGGGCCC CGGTGTACTT TGTTACCACC 
TTGGGCTACA ACTTCTCCAG CGAGGCTGGG ATGAATGCCA TCTGCTCCAG TGCAGGCTGC 
AACAACTTCT CCTTCACCCA GAAGATCCAG TATGCCACAG AGTTCCCTGA GCAGTCTTAC 
CTGGCCATCC CTGCCTCCTC CTGGGTGGAT GACTTCATTG ACTGGCTGAC CCCGTCCTCC 
TGCTGCCGCC TTTATATATC TGGCCCCAAT AAGGACAAGT TCTGCCCCTC GACCGTCAAC 
TCTCTGAACT GCCTAAAGAA CTGCATGAGC ATCACGATGG GCTCTGTGAG GCCCTCGGTG 
GAGCAGTTCC ATAAGTATCT TCCCTGGTTC CTGAACGACC GGCCCAACAT CAAATGTCCC 
AAAGGCGGCC TGGCAGCATA CAGCACCTCT GTGAACTTGA CTTCAGATGG CCAGGTTTTA 
GCCTCCAGGT TCATGGCCTA TCACAAGCCC CTGAAAAACT CACAGGATTA CACAGAAGCT 
CTGCGGGCAG CTCGAGAGCT GGCAGCCAAC ATCACTGCTG ACCTGCGGAA AGTGCCTGGA 
ACAGACCCGG CTTTTGAGGT CTTCCCCTAC ACGATCACCA ATGTGTTTTA TGAGCAGTAC 
CTGACCATCC TCCCTGAGGG GCTCTTCATG CTCAGCCTCT GCCTTGTGCC CACCTTCGCT 
GTCTCCTGCC TCCTGCTGGG CCTGGACCTG CGCTCCGGCC TCCTCAACCT GCTCTCCATT 
GTCATGATCC TCGTGGACAC TGTCGGCTTC ATGGCCCTGT GGGGCATCAG TTACAATGCT 
GTGTCCCTCA TCAACCTGGT CTCGGCGGTG GGCATGTCTG TGGAGTTTGT GTCCCACATT 
ACCCGCTCCT TTGCCATCAG CACCAAGCCC ACCTGGCTGG AGAGGGCCAA AGAGGCCACC 
ATCTCTATGG GAAGTGCGGT GTTTGCAGGT GTGGCCATGA CCAACCTGCC TGGCATCCTT 
GTCCTGGGCC TCGCCAAGGC CCAGCTCATT CAGATCTTCT TCTTCCGCCT CAACCTCCTG 
ATCACTCTGC TGGGCCTGCT GCATGGCTTG GTCTTCCTGC CCGTCATCCT CAGCTACGTG 
GGGCCTGACG TTAACCCGGC TCTGGCACTG GAGCAGAAGC GGGCTGAGGA GGCGGTGGCA 
GCAGTCATGG TGGCCTCTTG CCCAAATCAC CCCTCCCGAG TCTCCACAGC TGACAACATC 
TATGTCAACC ACAGCTTTGA AGGTTCTATC AAAGGTGCTG GTGCCATCAG CAACTTCTTG 
CCCAACAATG GGCGGCAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NPC1L1
Entrez Gene ID	29881
Full Name	NPC1 like intracellular cholesterol transporter 1
Synonyms	NPC11L1,SLC65A2
General protein information	Preferred Names NPC1 like intracellular cholesterol transporter 1 Names Niemann-Pick C1-like protein 1 NPC1 (Niemann-Pick disease, type C1, gene)-like 1 NPC1 like 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p13
Summary	The protein encoded by this gene is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009].
Disorder MIM:	608010
Disorder Html:	[Ezetimibe, nonresponse to] (3)

Bos taurus (cattle)	NPC1L1	XP_002686936.1
Mus musculus (house mouse)	Npc1l1	NP_997125.2
Homo sapiens (human)	NPC1L1	NP_037521.2
Canis lupus familiaris (dog)	NPC1L1	NP_001091019.1
Danio rerio (zebrafish)	npc1l1	XP_005171497.1
Xenopus tropicalis (tropical clawed frog)	npc1l1	XP_002932620.2
Pan troglodytes (chimpanzee)	NPC1L1	XP_003318442.1
	NPC1L1	NP_001071157.1
Rattus norvegicus (Norway rat)	Npc1l1	NP_001002025.1

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NPC1L1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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