GUCY2F cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GUCY2F
Entrez Gene ID	2986
Full Name	guanylate cyclase 2F, retinal
Synonyms	CYGF,GC-F,GUC2DL,GUC2F,RETGC-2,ROS-GC2
General protein information	Preferred Names guanylate cyclase 2F, retinal Names retinal guanylyl cyclase 2 guanylate cyclase 2D-like, membrane (retina-specific) guanylate cyclase F rod outer segment membrane guanylate cyclase 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq22.3-q23
Summary	The protein encoded by this gene is a guanylyl cyclase found predominantly in photoreceptors in the retina. The encoded protein is thought to be involved in resynthesis of cGMP after light activation of the visual signal transduction cascade, allowing a return to the dark state. This protein is a single-pass type I membrane protein. Defects in this gene may be a cause of X-linked retinitis pigmentosa. [provided by RefSeq, Dec 2008].
Disorder MIM:	300041

mRNA and Protein(s)

mRNA	Protein	Name
NM_001522.2	NP_001513.2	retinal guanylyl cyclase 2
NM_001522.3	NP_001513.2	retinal guanylyl cyclase 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GUCY2F	NP_776974.1
Gallus gallus (chicken)	GUCY2F	XP_417281.3
Xenopus tropicalis (tropical clawed frog)	gucy2f	XP_002931862.1
Homo sapiens (human)	GUCY2F	NP_001513.2
	GUCY2F	XP_001098846.1
Rattus norvegicus (Norway rat)	Gucy2f	NP_446283.1
Pan troglodytes (chimpanzee)	GUCY2F	XP_003317673.1
Mus musculus (house mouse)	Gucy2f	NP_001007577.1
Canis lupus familiaris (dog)	GUCY2F	XP_005641716.1
Danio rerio (zebrafish)	gc2	NP_001103165.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic mutations associated with cigarette smoking in pancreatic cancer.
Blackford A, Parmigiani G, Kensler TW, Wolfgang C, Jones S, Zhang X, Parsons DW, Lin JC, Leary RJ, Eshleman JR, Goggins M, Jaffee EM, Iacobuzio-Donahue CA, Maitra A, Klein A, Cameron JL, Olino K, Schulick R, Winter J, Vogelstein B, Velculescu VE, Kinzler KW, Hruban RH
Cancer research69(8)3681-8(2009 Apr)

Expression level and activity profile of membrane bound guanylate cyclase type 2 in rod outer segments.
Helten A, S?ftel W, Koch KW
Journal of neurochemistry103(4)1439-46(2007 Nov)

Somatic mutations of GUCY2F, EPHA3, and NTRK3 in human cancers.
Wood LD, Calhoun ES, Silliman N, Ptak J, Szabo S, Powell SM, Riggins GJ, Wang TL, Yan H, Gazdar A, Kern SE, Pennacchio L, Kinzler KW, Vogelstein B, Velculescu VE
Human mutation27(10)1060-1(2006 Oct)

The DNA sequence of the human X chromosome.
Ross MT, Grafham DV, Coffey AJ, Scherer S, McLay K, Muzny D, Platzer M, Howell GR, Burrows C, Bird CP, Frankish A, Lovell FL, Howe KL, Ashurst JL, Fulton RS, Sudbrak R, Wen G, Jones MC, Hurles ME, Andrews TD, Scott CE, Searle S, Ramser J, Whittaker A, Deadman R, Carter NP, Hunt SE, Chen R, Cree A, Gunaratne P, Havlak P, Hodgson A, Metzker ML, Richards S, Scott G, Steffen D, Sodergren E, Wheeler DA, Worley KC, Ainscough R, Ambrose KD, Ansari-Lari MA, Aradhya S, Ashwell RI, Babbage AK, Bagguley CL, Ballabio A, Banerjee R, Barker GE, Barlow KF, Barrett IP, Bates KN, Beare DM, Beasley H, Beasley O, Beck A, Bethel G, Blechschmidt K, Brady N, Bray-Allen S, Bridgeman AM, Brown AJ, Brown MJ, Bonnin D, Bruford EA, Buhay C, Burch P, Burford D, Burgess J, Burrill W, Burton J, Bye JM, Carder C, Carrel L, Chako J, Chapman JC, Chavez D, Chen E, Chen G, Chen Y, Chen Z, Chinault C, Ciccodicola A, Clark SY, Clarke G, Clee CM, Clegg S, Clerc-Blankenburg K, Clifford K, Cobley V, Cole CG, Conquer JS, Corby N, Connor RE, David R, Davies J, Davis C, Davis J, Delgado O, Deshazo D, Dhami P, Ding Y, Dinh H, Dodsworth S, Draper H, Dugan-Rocha S, Dunham A, Dunn M, Durbin KJ, Dutta I, Eades T, Ellwood M, Emery-Cohen A, Errington H, Evans KL, Faulkner L, Francis F, Frankland J, Fraser AE, Galgoczy P, Gilbert J, Gill R, Gl?ckner G, Gregory SG, Gribble S, Griffiths C, Grocock R, Gu Y, Gwilliam R, Hamilton C, Hart EA, Hawes A, Heath PD, Heitmann K, Hennig S, Hernandez J, Hinzmann B, Ho S, Hoffs M, Howden PJ, Huckle EJ, Hume J, Hunt PJ, Hunt AR, Isherwood J, Jacob L, Johnson D, Jones S, de Jong PJ, Joseph SS, Keenan S, Kelly S, Kershaw JK, Khan Z, Kioschis P, Klages S, Knights AJ, Kosiura A, Kovar-Smith C, Laird GK, Langford C, Lawlor S, Leversha M, Lewis L, Liu W, Lloyd C, Lloyd DM, Loulseged H, Loveland JE, Lovell JD, Lozado R, Lu J, Lyne R, Ma J, Maheshwari M, Matthews LH, McDowall J, McLaren S, McMurray A, Meidl P, Meitinger T, Milne S, Miner G, Mistry SL, Morgan M, Morris S, M?ller I, Mullikin JC, Nguyen N, Nordsiek G, Nyakatura G, O'Dell CN, Okwuonu G, Palmer S, Pandian R, Parker D, Parrish J, Pasternak S, Patel D, Pearce AV, Pearson DM, Pelan SE, Perez L, Porter KM, Ramsey Y, Reichwald K, Rhodes S, Ridler KA, Schlessinger D, Schueler MG, Sehra HK, Shaw-Smith C, Shen H, Sheridan EM, Shownkeen R, Skuce CD, Smith ML, Sotheran EC, Steingruber HE, Steward CA, Storey R, Swann RM, Swarbreck D, Tabor PE, Taudien S, Taylor T, Teague B, Thomas K, Thorpe A, Timms K, Tracey A, Trevanion S, Tromans AC, d'Urso M, Verduzco D, Villasana D, Waldron L, Wall M, Wang Q, Warren J, Warry GL, Wei X, West A, Whitehead SL, Whiteley MN, Wilkinson JE, Willey DL, Williams G, Williams L, Williamson A, Williamson H, Wilming L, Woodmansey RL, Wray PW, Yen J, Zhang J, Zhou J, Zoghbi H, Zorilla S, Buck D, Reinhardt R, Poustka A, Rosenthal A, Lehrach H, Meindl A, Minx PJ, Hillier LW, Willard HF, Wilson RK, Waterston RH, Rice CM, Vaudin M, Coulson A, Nelson DL, Weinstock G, Sulston JE, Durbin R, Hubbard T, Gibbs RA, Beck S, Rogers J, Bentley DR
Nature434(7031)325-37(2005 Mar)

The kinase homology domain of retinal guanylyl cyclases 1 and 2 specifies the affinity and cooperativity of interaction with guanylyl cyclase activating protein-2.
Laura RP, Hurley JB
Biochemistry37(32)11264-71(1998 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Genetic mutations associated with cigarette smoking in pancreatic cancer.

Mutants of GC-activating protein 2 modulated ROS-GC2 in a manner different from their action on ROS-GC1 indicating that the Ca2+ sensitivity of the Ca2+ sensor is controlled by the mode of target-sensor interaction.
Title: Expression level and activity profile of membrane bound guanylate cyclase type 2 in rod outer segments.

Results show GUCY2F gene was implicated in the pathogenesis of breast, lung, and pancreatic cancers and it may be useful targets for diagnostic and therapeutic intervention in selected patients.
Title: Somatic mutations of GUCY2F, EPHA3, and NTRK3 in human cancers.

The following GUCY2F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUCY2F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19301	NM_001522.3 Latest version!	Homo sapiens guanylate cyclase 2F, retinal (GUCY2F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
View Old Accession Versions >>
OHu19301	NM_001522.2	Homo sapiens guanylate cyclase 2F, retinal (GUCY2F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$783.30 $1119.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GUCY2F ( NM_001522.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001513.2 Homo sapiens guanylate cyclase 2F, retinal (GUCY2F), mRNA.

Price & Availability↑

CloneID	OHu19301
Clone ID Related Accession (Same CDS sequence)	NM_001522.2 , NM_001522.3
Accession Version	NM_001522.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	retinal guanylyl cyclase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL031387.4 and L37378.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_001522.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no transcript sequence was available to make the entire refseq consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: L37378.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2161674 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000218006.3/ ENSP00000218006.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGTTCCTGG GACTCGGGCG CTTTTCTCGC CTTGTTCTCT GGTTTGCGGC TTTCAGGAAA 
CTGCTGGGAC ACCATGGCCT TGCATCTGCC AAGTTCCTGT GGTGCTTGTG CCTTCTGTCT 
GTCATGTCCC TTCCGCAGCA GGTGTGGACA CTCCCCTACA AGATAGGGGT GGTGGGCCCT 
TGGGCTTGTG ATTCGCTGTT TTCAAAGGCC CTGCCTGAGG TTGCTGCGCG ATTAGCCATT 
GAGCGAATCA ACCGGGACCC ATCTTTTGAC CTGAGTTATT CTTTTGAATA CGTGATTCTC 
AATGAAGACT GCCAGACTTC GAGGGCTCTC TCCAGTTTCA TTTCCCACCA CCAGATGGCC 
TCAGGATTTA TTGGACCTAC CAACCCTGGC TACTGCGAGG CAGCCTCGCT CCTGGGAAAC 
AGCTGGGACA AAGGAATTTT CTCTTGGGCT TGTGTGAATT ATGAATTAGA CAATAAAATT 
AGCTACCCGA CCTTTTCTCG GACACTCCCT TCTCCCATCC GGGTGCTTGT AACTGTCATG 
AAATATTTCC AGTGGGCTCA TGCTGGAGTC ATTTCCTCAG ATGAAGACAT TTGGGTGCAT 
ACAGCCAATC GAGTCGCAAG TGCTCTTCGG AGCCACGGCT TACCTGTAGG GGTCGTCCTG 
ACCACAGGAC AAGACAGCCA AAGCATGCGG AAAGCCCTCC AGAGGATTCA CCAGGCAGAC 
AGAATTCGCA TAATCATCAT GTGTATGCAT TCAGCTTTGA TTGGGGGAGA GACTCAGATG 
CATCTCTTGG AATGTGCTCA TGATCTGAAA ATGACTGATG GAACCTACGT CTTTGTTCCT 
TATGATGCCC TGCTCTACAG TTTACCTTAT AAGCACACCC CCTACCGGGT CCTAAGGAAC 
AACCCAAAGC TCCGGGAAGC CTATGATGCA GTGTTGACCA TTACAGTGGA GTCCCAAGAA 
AAGACCTTCT ATCAAGCCTT CACAGAGGCA GCAGCAAGAG GTGAAATTCC TGAGAAGCTG 
GAGTTCGATC AAGTTTCACC GTTGTTTGGA ACCATCTACA ATTCAATTTA CTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAGAAAAT GGACAGGCTG GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CCATGGATTC AACCAGTTGA TGAGGACAGA TTCAAATGGA 
AATGGAATTT CAGAATATGT AATCCTGGAC ACCAACTTGA AAGAATGGGA ACTCCATAGC 
ACCTACACTG TGGACATGGA AATGGAGCTG CTACGTTTCG GAGGGACCCC TATTCACTTC 
CCTGGTGGCA GGCCCCCTAG AGCAGATGCA AAATGCTGGT TTGCAGAAGG GAAGATCTGC 
CATGGAGGCA TCGACCCTGC CTTTGCCATG ATGGTCTGCC TTACTTTGCT TATAGCCCTG 
CTGTCTATTA ATGGATTTGC TTACTTTATA AGGCGTCGTA TAAATAAAAT CCAGTTGATC 
AAAGGACCCA ATAGAATTCT ACTGACTTTG GAGGATGTAA CGTTTATCAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGTCG TGCCAGTGTA AGCTTCCAGA TTACCTCAGA GGTCCAAAGT 
GGGAGGTCCC CAAGACTCTC CTTTTCTTCA GGGAGTCTAA CTCCAGCTAC CTATGAAAAC 
TCCAACATAG CGATTTATGA GGGTGATTGG GTGTGGCTGA AAAAGTTCTC CCTTGGAGAT 
TTTGGAGACC TTAAGTCCAT CAAATCAAGA GCAAGTGATG TGTTCGAAAT GATGAAGGAC 
TTGCGTCATG AGAATATTAA CCCTTTATTG GGTTTCTTCT ATGATTCGGG GATGTTTGCC 
ATTGTGACAG AATTCTGTTC CCGAGGGAGC CTAGAAGACA TACTGACAAA TCAAGATGTG 
AAACTTGACT GGATGTTTAA ATCATCACTC TTGCTGGATC TCATAAAGGG CATGAAGTAC 
TTACACCACA GAGAGTTTGT TCATGGGAGG CTAAAGTCTC GAAACTGTGT GGTAGATGGG 
CGTTTTGTAC TAAAAGTGAC AGATTATGGC TTTAACGACA TCTTAGAAAT GCTGAGACTC 
TCTGAAGAGG AATCTTCTAT GGAAGAGCTG CTGTGGACGG CCCCTGAACT GTTGAGAGCT 
CCAAGAGGCA GCAGGTTAGG TTCTTTTGCA GGAGATGTCT ATAGCTTTGC CATCATCATG 
CAAGAAGTGA TGGTCCGGGG TACCCCATTC TGCATGATGG ATCTGCCAGC TCAAGAAATC 
ATAAACAGAC TTAAGAAGCC TCCTCCTGTG TACAGACCAG TAGTTCCTCC TGAGCATGCC 
CCTCCAGAAT GTCTCCAGCT GATGAAGCAG TGCTGGGCTG AGGCTGCAGA ACAACGACCA 
ACTTTTGATG AAATATTTAA CCAGTTTAAA ACTTTTAATA AAGGGAAGAA GACCAATATT 
ATTGATTCTA TGCTTCGGAT GTTGGAGCAA TATTCTAGCA ACTTGGAAGA TTTGATTCGG 
GAGCGGACTG AAGAGCTGGA AATTGAAAAA CAGAAAACGG AAAAGCTTCT AACACAGATG 
CTACCACCAT CAGTTGCTGA ATCTCTCAAA AAGGGCTGCA CAGTTGAACC TGAGGGCTTT 
GACTTGGTCA CCTTGTACTT CAGCGACATT GTGGGCTTCA CAACCATTTC AGCCATGAGT 
GAGCCCATTG AGGTCGTGGA TCTTCTGAAT GACCTGTACA CACTCTTTGA TGCAATAATT 
GGCAGTCATG ATGTCTACAA GGTAGAGACC ATTGGAGATG CCTACATGGT GGCTTCAGGC 
CTCCCAAAGA GGAATGGCAG TAGGCATGCA GCTGAGATTG CAAACATGTC CTTAGATATC 
CTGAGCTCTG TGGGCACTTT CAAGATGCGG CACATGCCAG AAGTGCCGGT CCGAATTCGA 
ATTGGCCTTC ACTCAGGGCC GGTTGTTGCT GGAGTGGTGG GCCTCACCAT GCCCAGATAC 
TGCTTGTTTG GAGACACTGT GAACACAGCT TCTCGGATGG AATCTACAGG CTTACCTTAT 
CGCATTCATG TCAGTCTCAG CACTGTTACA ATTCTTCAAA ATCTGAGTGA GGGCTATGAA 
GTGGAGCTTC GAGGAAGAAC AGAGCTCAAG GGCAAAGGCA CAGAGGAAAC CTTCTGGCTG 
ATTGGGAAAA AAGGCTTCAT GAAGCCCCTT CCTGTGCCCC CACCAGTGGA CAAAGATGGG 
CAAGTGGGCC ATGGCCTGCA ACCAGTGGAG ATTGCAGCCT TCCAAAGAAG AAAAGCAGAA 
AGGCAGTTGG TGAGAAACAA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001513.2
CDS	307..3633
Translation	MFLGLGRFSRLVLWFAAFRKLLGHHGLASAKFLWCLCLLSVMSLPQQVWTLPYKIGVVGPWACDSLFSKALPEVAARLAIERINRDPSFDLSYSFEYVILNEDCQTSRALSSFISHHQMASGFIGPTNPGYCEAASLLGNSWDKGIFSWACVNYELDNKISYPTFSRTLPSPIRVLVTVMKYFQWAHAGVISSDEDIWVHTANRVASALRSHGLPVGVVLTTGQDSQSMRKALQRIHQADRIRIIIMCMHSALIGGETQMHLLECAHDLKMTDGTYVFVPYDALLYSLPYKHTPYRVLRNNPKLREAYDAVLTITVESQEKTFYQAFTEAAARGEIPEKLEFDQVSPLFGTIYNSIYFIAQAMNNAMKENGQAGAASLVQHSRNMQFHGFNQLMRTDSNGNGISEYVILDTNLKEWELHSTYTVDMEMELLRFGGTPIHFPGGRPPRADAKCWFAEGKICHGGIDPAFAMMVCLTLLIALLSINGFAYFIRRRINKIQLIKGPNRILLTLEDVTFINPHFGSKRGSRASVSFQITSEVQSGRSPRLSFSSGSLTPATYENSNIAIYEGDWVWLKKFSLGDFGDLKSIKSRASDVFEMMKDLRHENINPLLGFFYDSGMFAIVTEFCSRGSLEDILTNQDVKLDWMFKSSLLLDLIKGMKYLHHREFVHGRLKSRNCVVDGRFVLKVTDYGFNDILEMLRLSEEESSMEELLWTAPELLRAPRGSRLGSFAGDVYSFAIIMQEVMVRGTPFCMMDLPAQEIINRLKKPPPVYRPVVPPEHAPPECLQLMKQCWAEAAEQRPTFDEIFNQFKTFNKGKKTNIIDSMLRMLEQYSSNLEDLIRERTEELEIEKQKTEKLLTQMLPPSVAESLKKGCTVEPEGFDLVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPKRNGSRHAAEIANMSLDILSSVGTFKMRHMPEVPVRIRIGLHSGPVVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVSLSTVTILQNLSEGYEVELRGRTELKGKGTEETFWLIGKKGFMKPLPVPPPVDKDGQVGHGLQPVEIAAFQRRKAERQLVRNKP

Target ORF information:

RefSeq Version	NM_001522.3
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 2F, retinal (GUCY2F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001522.3

ORF Insert Sequence:

ATGTTCCTGG GACTCGGGCG CTTTTCTCGC CTTGTTCTCT GGTTTGCGGC TTTCAGGAAA 
CTGCTGGGAC ACCATGGCCT TGCATCTGCC AAGTTCCTGT GGTGCTTGTG CCTTCTGTCT 
GTCATGTCCC TTCCGCAGCA GGTGTGGACA CTCCCCTACA AGATAGGGGT GGTGGGCCCT 
TGGGCTTGTG ATTCGCTGTT TTCAAAGGCC CTGCCTGAGG TTGCTGCGCG ATTAGCCATT 
GAGCGAATCA ACCGGGACCC ATCTTTTGAC CTGAGTTATT CTTTTGAATA CGTGATTCTC 
AATGAAGACT GCCAGACTTC GAGGGCTCTC TCCAGTTTCA TTTCCCACCA CCAGATGGCC 
TCAGGATTTA TTGGACCTAC CAACCCTGGC TACTGCGAGG CAGCCTCGCT CCTGGGAAAC 
AGCTGGGACA AAGGAATTTT CTCTTGGGCT TGTGTGAATT ATGAATTAGA CAATAAAATT 
AGCTACCCGA CCTTTTCTCG GACACTCCCT TCTCCCATCC GGGTGCTTGT AACTGTCATG 
AAATATTTCC AGTGGGCTCA TGCTGGAGTC ATTTCCTCAG ATGAAGACAT TTGGGTGCAT 
ACAGCCAATC GAGTCGCAAG TGCTCTTCGG AGCCACGGCT TACCTGTAGG GGTCGTCCTG 
ACCACAGGAC AAGACAGCCA AAGCATGCGG AAAGCCCTCC AGAGGATTCA CCAGGCAGAC 
AGAATTCGCA TAATCATCAT GTGTATGCAT TCAGCTTTGA TTGGGGGAGA GACTCAGATG 
CATCTCTTGG AATGTGCTCA TGATCTGAAA ATGACTGATG GAACCTACGT CTTTGTTCCT 
TATGATGCCC TGCTCTACAG TTTACCTTAT AAGCACACCC CCTACCGGGT CCTAAGGAAC 
AACCCAAAGC TCCGGGAAGC CTATGATGCA GTGTTGACCA TTACAGTGGA GTCCCAAGAA 
AAGACCTTCT ATCAAGCCTT CACAGAGGCA GCAGCAAGAG GTGAAATTCC TGAGAAGCTG 
GAGTTCGATC AAGTTTCACC GTTGTTTGGA ACCATCTACA ATTCAATTTA CTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAGAAAAT GGACAGGCTG GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CCATGGATTC AACCAGTTGA TGAGGACAGA TTCAAATGGA 
AATGGAATTT CAGAATATGT AATCCTGGAC ACCAACTTGA AAGAATGGGA ACTCCATAGC 
ACCTACACTG TGGACATGGA AATGGAGCTG CTACGTTTCG GAGGGACCCC TATTCACTTC 
CCTGGTGGCA GGCCCCCTAG AGCAGATGCA AAATGCTGGT TTGCAGAAGG GAAGATCTGC 
CATGGAGGCA TCGACCCTGC CTTTGCCATG ATGGTCTGCC TTACTTTGCT TATAGCCCTG 
CTGTCTATTA ATGGATTTGC TTACTTTATA AGGCGTCGTA TAAATAAAAT CCAGTTGATC 
AAAGGACCCA ATAGAATTCT ACTGACTTTG GAGGATGTAA CGTTTATCAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGTCG TGCCAGTGTA AGCTTCCAGA TTACCTCAGA GGTCCAAAGT 
GGGAGGTCCC CAAGACTCTC CTTTTCTTCA GGGAGTCTAA CTCCAGCTAC CTATGAAAAC 
TCCAACATAG CGATTTATGA GGGTGATTGG GTGTGGCTGA AAAAGTTCTC CCTTGGAGAT 
TTTGGAGACC TTAAGTCCAT CAAATCAAGA GCAAGTGATG TGTTCGAAAT GATGAAGGAC 
TTGCGTCATG AGAATATTAA CCCTTTATTG GGTTTCTTCT ATGATTCGGG GATGTTTGCC 
ATTGTGACAG AATTCTGTTC CCGAGGGAGC CTAGAAGACA TACTGACAAA TCAAGATGTG 
AAACTTGACT GGATGTTTAA ATCATCACTC TTGCTGGATC TCATAAAGGG CATGAAGTAC 
TTACACCACA GAGAGTTTGT TCATGGGAGG CTAAAGTCTC GAAACTGTGT GGTAGATGGG 
CGTTTTGTAC TAAAAGTGAC AGATTATGGC TTTAACGACA TCTTAGAAAT GCTGAGACTC 
TCTGAAGAGG AATCTTCTAT GGAAGAGCTG CTGTGGACGG CCCCTGAACT GTTGAGAGCT 
CCAAGAGGCA GCAGGTTAGG TTCTTTTGCA GGAGATGTCT ATAGCTTTGC CATCATCATG 
CAAGAAGTGA TGGTCCGGGG TACCCCATTC TGCATGATGG ATCTGCCAGC TCAAGAAATC 
ATAAACAGAC TTAAGAAGCC TCCTCCTGTG TACAGACCAG TAGTTCCTCC TGAGCATGCC 
CCTCCAGAAT GTCTCCAGCT GATGAAGCAG TGCTGGGCTG AGGCTGCAGA ACAACGACCA 
ACTTTTGATG AAATATTTAA CCAGTTTAAA ACTTTTAATA AAGGGAAGAA GACCAATATT 
ATTGATTCTA TGCTTCGGAT GTTGGAGCAA TATTCTAGCA ACTTGGAAGA TTTGATTCGG 
GAGCGGACTG AAGAGCTGGA AATTGAAAAA CAGAAAACGG AAAAGCTTCT AACACAGATG 
CTACCACCAT CAGTTGCTGA ATCTCTCAAA AAGGGCTGCA CAGTTGAACC TGAGGGCTTT 
GACTTGGTCA CCTTGTACTT CAGCGACATT GTGGGCTTCA CAACCATTTC AGCCATGAGT 
GAGCCCATTG AGGTCGTGGA TCTTCTGAAT GACCTGTACA CACTCTTTGA TGCAATAATT 
GGCAGTCATG ATGTCTACAA GGTAGAGACC ATTGGAGATG CCTACATGGT GGCTTCAGGC 
CTCCCAAAGA GGAATGGCAG TAGGCATGCA GCTGAGATTG CAAACATGTC CTTAGATATC 
CTGAGCTCTG TGGGCACTTT CAAGATGCGG CACATGCCAG AAGTGCCGGT CCGAATTCGA 
ATTGGCCTTC ACTCAGGGCC GGTTGTTGCT GGAGTGGTGG GCCTCACCAT GCCCAGATAC 
TGCTTGTTTG GAGACACTGT GAACACAGCT TCTCGGATGG AATCTACAGG CTTACCTTAT 
CGCATTCATG TCAGTCTCAG CACTGTTACA ATTCTTCAAA ATCTGAGTGA GGGCTATGAA 
GTGGAGCTTC GAGGAAGAAC AGAGCTCAAG GGCAAAGGCA CAGAGGAAAC CTTCTGGCTG 
ATTGGGAAAA AAGGCTTCAT GAAGCCCCTT CCTGTGCCCC CACCAGTGGA CAAAGATGGG 
CAAGTGGGCC ATGGCCTGCA ACCAGTGGAG ATTGCAGCCT TCCAAAGAAG AAAAGCAGAA 
AGGCAGTTGG TGAGAAACAA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY2F ( NM_001522.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001513.2 Homo sapiens guanylate cyclase 2F, retinal (GUCY2F), mRNA.

Price & Availability↑

CloneID	OHu19301
Clone ID Related Accession (Same CDS sequence)	NM_001522.2 , NM_001522.3
Accession Version	NM_001522.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	retinal guanylyl cyclase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L37378.1 and AL031387.4. This sequence is a reference standard in the RefSeqGene project. On Mar 28, 2007 this sequence version replaced NM_001522.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no transcript sequence was available to make the entire refseq consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: L37378.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2161674 [ECO:0000350] ##Evidence-Data-END##

ATGTTCCTGG GACTCGGGCG CTTTTCTCGC CTTGTTCTCT GGTTTGCGGC TTTCAGGAAA 
CTGCTGGGAC ACCATGGCCT TGCATCTGCC AAGTTCCTGT GGTGCTTGTG CCTTCTGTCT 
GTCATGTCCC TTCCGCAGCA GGTGTGGACA CTCCCCTACA AGATAGGGGT GGTGGGCCCT 
TGGGCTTGTG ATTCGCTGTT TTCAAAGGCC CTGCCTGAGG TTGCTGCGCG ATTAGCCATT 
GAGCGAATCA ACCGGGACCC ATCTTTTGAC CTGAGTTATT CTTTTGAATA CGTGATTCTC 
AATGAAGACT GCCAGACTTC GAGGGCTCTC TCCAGTTTCA TTTCCCACCA CCAGATGGCC 
TCAGGATTTA TTGGACCTAC CAACCCTGGC TACTGCGAGG CAGCCTCGCT CCTGGGAAAC 
AGCTGGGACA AAGGAATTTT CTCTTGGGCT TGTGTGAATT ATGAATTAGA CAATAAAATT 
AGCTACCCGA CCTTTTCTCG GACACTCCCT TCTCCCATCC GGGTGCTTGT AACTGTCATG 
AAATATTTCC AGTGGGCTCA TGCTGGAGTC ATTTCCTCAG ATGAAGACAT TTGGGTGCAT 
ACAGCCAATC GAGTCGCAAG TGCTCTTCGG AGCCACGGCT TACCTGTAGG GGTCGTCCTG 
ACCACAGGAC AAGACAGCCA AAGCATGCGG AAAGCCCTCC AGAGGATTCA CCAGGCAGAC 
AGAATTCGCA TAATCATCAT GTGTATGCAT TCAGCTTTGA TTGGGGGAGA GACTCAGATG 
CATCTCTTGG AATGTGCTCA TGATCTGAAA ATGACTGATG GAACCTACGT CTTTGTTCCT 
TATGATGCCC TGCTCTACAG TTTACCTTAT AAGCACACCC CCTACCGGGT CCTAAGGAAC 
AACCCAAAGC TCCGGGAAGC CTATGATGCA GTGTTGACCA TTACAGTGGA GTCCCAAGAA 
AAGACCTTCT ATCAAGCCTT CACAGAGGCA GCAGCAAGAG GTGAAATTCC TGAGAAGCTG 
GAGTTCGATC AAGTTTCACC GTTGTTTGGA ACCATCTACA ATTCAATTTA CTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAGAAAAT GGACAGGCTG GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CCATGGATTC AACCAGTTGA TGAGGACAGA TTCAAATGGA 
AATGGAATTT CAGAATATGT AATCCTGGAC ACCAACTTGA AAGAATGGGA ACTCCATAGC 
ACCTACACTG TGGACATGGA AATGGAGCTG CTACGTTTCG GAGGGACCCC TATTCACTTC 
CCTGGTGGCA GGCCCCCTAG AGCAGATGCA AAATGCTGGT TTGCAGAAGG GAAGATCTGC 
CATGGAGGCA TCGACCCTGC CTTTGCCATG ATGGTCTGCC TTACTTTGCT TATAGCCCTG 
CTGTCTATTA ATGGATTTGC TTACTTTATA AGGCGTCGTA TAAATAAAAT CCAGTTGATC 
AAAGGACCCA ATAGAATTCT ACTGACTTTG GAGGATGTAA CGTTTATCAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGTCG TGCCAGTGTA AGCTTCCAGA TTACCTCAGA GGTCCAAAGT 
GGGAGGTCCC CAAGACTCTC CTTTTCTTCA GGGAGTCTAA CTCCAGCTAC CTATGAAAAC 
TCCAACATAG CGATTTATGA GGGTGATTGG GTGTGGCTGA AAAAGTTCTC CCTTGGAGAT 
TTTGGAGACC TTAAGTCCAT CAAATCAAGA GCAAGTGATG TGTTCGAAAT GATGAAGGAC 
TTGCGTCATG AGAATATTAA CCCTTTATTG GGTTTCTTCT ATGATTCGGG GATGTTTGCC 
ATTGTGACAG AATTCTGTTC CCGAGGGAGC CTAGAAGACA TACTGACAAA TCAAGATGTG 
AAACTTGACT GGATGTTTAA ATCATCACTC TTGCTGGATC TCATAAAGGG CATGAAGTAC 
TTACACCACA GAGAGTTTGT TCATGGGAGG CTAAAGTCTC GAAACTGTGT GGTAGATGGG 
CGTTTTGTAC TAAAAGTGAC AGATTATGGC TTTAACGACA TCTTAGAAAT GCTGAGACTC 
TCTGAAGAGG AATCTTCTAT GGAAGAGCTG CTGTGGACGG CCCCTGAACT GTTGAGAGCT 
CCAAGAGGCA GCAGGTTAGG TTCTTTTGCA GGAGATGTCT ATAGCTTTGC CATCATCATG 
CAAGAAGTGA TGGTCCGGGG TACCCCATTC TGCATGATGG ATCTGCCAGC TCAAGAAATC 
ATAAACAGAC TTAAGAAGCC TCCTCCTGTG TACAGACCAG TAGTTCCTCC TGAGCATGCC 
CCTCCAGAAT GTCTCCAGCT GATGAAGCAG TGCTGGGCTG AGGCTGCAGA ACAACGACCA 
ACTTTTGATG AAATATTTAA CCAGTTTAAA ACTTTTAATA AAGGGAAGAA GACCAATATT 
ATTGATTCTA TGCTTCGGAT GTTGGAGCAA TATTCTAGCA ACTTGGAAGA TTTGATTCGG 
GAGCGGACTG AAGAGCTGGA AATTGAAAAA CAGAAAACGG AAAAGCTTCT AACACAGATG 
CTACCACCAT CAGTTGCTGA ATCTCTCAAA AAGGGCTGCA CAGTTGAACC TGAGGGCTTT 
GACTTGGTCA CCTTGTACTT CAGCGACATT GTGGGCTTCA CAACCATTTC AGCCATGAGT 
GAGCCCATTG AGGTCGTGGA TCTTCTGAAT GACCTGTACA CACTCTTTGA TGCAATAATT 
GGCAGTCATG ATGTCTACAA GGTAGAGACC ATTGGAGATG CCTACATGGT GGCTTCAGGC 
CTCCCAAAGA GGAATGGCAG TAGGCATGCA GCTGAGATTG CAAACATGTC CTTAGATATC 
CTGAGCTCTG TGGGCACTTT CAAGATGCGG CACATGCCAG AAGTGCCGGT CCGAATTCGA 
ATTGGCCTTC ACTCAGGGCC GGTTGTTGCT GGAGTGGTGG GCCTCACCAT GCCCAGATAC 
TGCTTGTTTG GAGACACTGT GAACACAGCT TCTCGGATGG AATCTACAGG CTTACCTTAT 
CGCATTCATG TCAGTCTCAG CACTGTTACA ATTCTTCAAA ATCTGAGTGA GGGCTATGAA 
GTGGAGCTTC GAGGAAGAAC AGAGCTCAAG GGCAAAGGCA CAGAGGAAAC CTTCTGGCTG 
ATTGGGAAAA AAGGCTTCAT GAAGCCCCTT CCTGTGCCCC CACCAGTGGA CAAAGATGGG 
CAAGTGGGCC ATGGCCTGCA ACCAGTGGAG ATTGCAGCCT TCCAAAGAAG AAAAGCAGAA 
AGGCAGTTGG TGAGAAACAA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001513.2
CDS	277..3603
Translation	MFLGLGRFSRLVLWFAAFRKLLGHHGLASAKFLWCLCLLSVMSLPQQVWTLPYKIGVVGPWACDSLFSKALPEVAARLAIERINRDPSFDLSYSFEYVILNEDCQTSRALSSFISHHQMASGFIGPTNPGYCEAASLLGNSWDKGIFSWACVNYELDNKISYPTFSRTLPSPIRVLVTVMKYFQWAHAGVISSDEDIWVHTANRVASALRSHGLPVGVVLTTGQDSQSMRKALQRIHQADRIRIIIMCMHSALIGGETQMHLLECAHDLKMTDGTYVFVPYDALLYSLPYKHTPYRVLRNNPKLREAYDAVLTITVESQEKTFYQAFTEAAARGEIPEKLEFDQVSPLFGTIYNSIYFIAQAMNNAMKENGQAGAASLVQHSRNMQFHGFNQLMRTDSNGNGISEYVILDTNLKEWELHSTYTVDMEMELLRFGGTPIHFPGGRPPRADAKCWFAEGKICHGGIDPAFAMMVCLTLLIALLSINGFAYFIRRRINKIQLIKGPNRILLTLEDVTFINPHFGSKRGSRASVSFQITSEVQSGRSPRLSFSSGSLTPATYENSNIAIYEGDWVWLKKFSLGDFGDLKSIKSRASDVFEMMKDLRHENINPLLGFFYDSGMFAIVTEFCSRGSLEDILTNQDVKLDWMFKSSLLLDLIKGMKYLHHREFVHGRLKSRNCVVDGRFVLKVTDYGFNDILEMLRLSEEESSMEELLWTAPELLRAPRGSRLGSFAGDVYSFAIIMQEVMVRGTPFCMMDLPAQEIINRLKKPPPVYRPVVPPEHAPPECLQLMKQCWAEAAEQRPTFDEIFNQFKTFNKGKKTNIIDSMLRMLEQYSSNLEDLIRERTEELEIEKQKTEKLLTQMLPPSVAESLKKGCTVEPEGFDLVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPKRNGSRHAAEIANMSLDILSSVGTFKMRHMPEVPVRIRIGLHSGPVVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVSLSTVTILQNLSEGYEVELRGRTELKGKGTEETFWLIGKKGFMKPLPVPPPVDKDGQVGHGLQPVEIAAFQRRKAERQLVRNKP

Target ORF information:

RefSeq Version	NM_001522.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 2F, retinal (GUCY2F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001522.2

ORF Insert Sequence:

ATGTTCCTGG GACTCGGGCG CTTTTCTCGC CTTGTTCTCT GGTTTGCGGC TTTCAGGAAA 
CTGCTGGGAC ACCATGGCCT TGCATCTGCC AAGTTCCTGT GGTGCTTGTG CCTTCTGTCT 
GTCATGTCCC TTCCGCAGCA GGTGTGGACA CTCCCCTACA AGATAGGGGT GGTGGGCCCT 
TGGGCTTGTG ATTCGCTGTT TTCAAAGGCC CTGCCTGAGG TTGCTGCGCG ATTAGCCATT 
GAGCGAATCA ACCGGGACCC ATCTTTTGAC CTGAGTTATT CTTTTGAATA CGTGATTCTC 
AATGAAGACT GCCAGACTTC GAGGGCTCTC TCCAGTTTCA TTTCCCACCA CCAGATGGCC 
TCAGGATTTA TTGGACCTAC CAACCCTGGC TACTGCGAGG CAGCCTCGCT CCTGGGAAAC 
AGCTGGGACA AAGGAATTTT CTCTTGGGCT TGTGTGAATT ATGAATTAGA CAATAAAATT 
AGCTACCCGA CCTTTTCTCG GACACTCCCT TCTCCCATCC GGGTGCTTGT AACTGTCATG 
AAATATTTCC AGTGGGCTCA TGCTGGAGTC ATTTCCTCAG ATGAAGACAT TTGGGTGCAT 
ACAGCCAATC GAGTCGCAAG TGCTCTTCGG AGCCACGGCT TACCTGTAGG GGTCGTCCTG 
ACCACAGGAC AAGACAGCCA AAGCATGCGG AAAGCCCTCC AGAGGATTCA CCAGGCAGAC 
AGAATTCGCA TAATCATCAT GTGTATGCAT TCAGCTTTGA TTGGGGGAGA GACTCAGATG 
CATCTCTTGG AATGTGCTCA TGATCTGAAA ATGACTGATG GAACCTACGT CTTTGTTCCT 
TATGATGCCC TGCTCTACAG TTTACCTTAT AAGCACACCC CCTACCGGGT CCTAAGGAAC 
AACCCAAAGC TCCGGGAAGC CTATGATGCA GTGTTGACCA TTACAGTGGA GTCCCAAGAA 
AAGACCTTCT ATCAAGCCTT CACAGAGGCA GCAGCAAGAG GTGAAATTCC TGAGAAGCTG 
GAGTTCGATC AAGTTTCACC GTTGTTTGGA ACCATCTACA ATTCAATTTA CTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAGAAAAT GGACAGGCTG GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CCATGGATTC AACCAGTTGA TGAGGACAGA TTCAAATGGA 
AATGGAATTT CAGAATATGT AATCCTGGAC ACCAACTTGA AAGAATGGGA ACTCCATAGC 
ACCTACACTG TGGACATGGA AATGGAGCTG CTACGTTTCG GAGGGACCCC TATTCACTTC 
CCTGGTGGCA GGCCCCCTAG AGCAGATGCA AAATGCTGGT TTGCAGAAGG GAAGATCTGC 
CATGGAGGCA TCGACCCTGC CTTTGCCATG ATGGTCTGCC TTACTTTGCT TATAGCCCTG 
CTGTCTATTA ATGGATTTGC TTACTTTATA AGGCGTCGTA TAAATAAAAT CCAGTTGATC 
AAAGGACCCA ATAGAATTCT ACTGACTTTG GAGGATGTAA CGTTTATCAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGTCG TGCCAGTGTA AGCTTCCAGA TTACCTCAGA GGTCCAAAGT 
GGGAGGTCCC CAAGACTCTC CTTTTCTTCA GGGAGTCTAA CTCCAGCTAC CTATGAAAAC 
TCCAACATAG CGATTTATGA GGGTGATTGG GTGTGGCTGA AAAAGTTCTC CCTTGGAGAT 
TTTGGAGACC TTAAGTCCAT CAAATCAAGA GCAAGTGATG TGTTCGAAAT GATGAAGGAC 
TTGCGTCATG AGAATATTAA CCCTTTATTG GGTTTCTTCT ATGATTCGGG GATGTTTGCC 
ATTGTGACAG AATTCTGTTC CCGAGGGAGC CTAGAAGACA TACTGACAAA TCAAGATGTG 
AAACTTGACT GGATGTTTAA ATCATCACTC TTGCTGGATC TCATAAAGGG CATGAAGTAC 
TTACACCACA GAGAGTTTGT TCATGGGAGG CTAAAGTCTC GAAACTGTGT GGTAGATGGG 
CGTTTTGTAC TAAAAGTGAC AGATTATGGC TTTAACGACA TCTTAGAAAT GCTGAGACTC 
TCTGAAGAGG AATCTTCTAT GGAAGAGCTG CTGTGGACGG CCCCTGAACT GTTGAGAGCT 
CCAAGAGGCA GCAGGTTAGG TTCTTTTGCA GGAGATGTCT ATAGCTTTGC CATCATCATG 
CAAGAAGTGA TGGTCCGGGG TACCCCATTC TGCATGATGG ATCTGCCAGC TCAAGAAATC 
ATAAACAGAC TTAAGAAGCC TCCTCCTGTG TACAGACCAG TAGTTCCTCC TGAGCATGCC 
CCTCCAGAAT GTCTCCAGCT GATGAAGCAG TGCTGGGCTG AGGCTGCAGA ACAACGACCA 
ACTTTTGATG AAATATTTAA CCAGTTTAAA ACTTTTAATA AAGGGAAGAA GACCAATATT 
ATTGATTCTA TGCTTCGGAT GTTGGAGCAA TATTCTAGCA ACTTGGAAGA TTTGATTCGG 
GAGCGGACTG AAGAGCTGGA AATTGAAAAA CAGAAAACGG AAAAGCTTCT AACACAGATG 
CTACCACCAT CAGTTGCTGA ATCTCTCAAA AAGGGCTGCA CAGTTGAACC TGAGGGCTTT 
GACTTGGTCA CCTTGTACTT CAGCGACATT GTGGGCTTCA CAACCATTTC AGCCATGAGT 
GAGCCCATTG AGGTCGTGGA TCTTCTGAAT GACCTGTACA CACTCTTTGA TGCAATAATT 
GGCAGTCATG ATGTCTACAA GGTAGAGACC ATTGGAGATG CCTACATGGT GGCTTCAGGC 
CTCCCAAAGA GGAATGGCAG TAGGCATGCA GCTGAGATTG CAAACATGTC CTTAGATATC 
CTGAGCTCTG TGGGCACTTT CAAGATGCGG CACATGCCAG AAGTGCCGGT CCGAATTCGA 
ATTGGCCTTC ACTCAGGGCC GGTTGTTGCT GGAGTGGTGG GCCTCACCAT GCCCAGATAC 
TGCTTGTTTG GAGACACTGT GAACACAGCT TCTCGGATGG AATCTACAGG CTTACCTTAT 
CGCATTCATG TCAGTCTCAG CACTGTTACA ATTCTTCAAA ATCTGAGTGA GGGCTATGAA 
GTGGAGCTTC GAGGAAGAAC AGAGCTCAAG GGCAAAGGCA CAGAGGAAAC CTTCTGGCTG 
ATTGGGAAAA AAGGCTTCAT GAAGCCCCTT CCTGTGCCCC CACCAGTGGA CAAAGATGGG 
CAAGTGGGCC ATGGCCTGCA ACCAGTGGAG ATTGCAGCCT TCCAAAGAAG AAAAGCAGAA 
AGGCAGTTGG TGAGAAACAA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic mutations associated with cigarette smoking in pancreatic cancer.
Cancer research69(8)3681-8(2009 Apr)
Blackford A,Parmigiani G,Kensler TW,Wolfgang C,Jones S,Zhang X,Parsons DW,Lin JC,Leary RJ,Eshleman JR,Goggins M,Jaffee EM,Iacobuzio-Donahue CA,Maitra A,Klein A,Cameron JL,Olino K,Schulick R,Winter J,Vogelstein B,Velculescu VE,Kinzler KW,Hruban RH

Expression level and activity profile of membrane bound guanylate cyclase type 2 in rod outer segments.
Journal of neurochemistry103(4)1439-46(2007 Nov)
Helten A,S?ftel W,Koch KW

Somatic mutations of GUCY2F, EPHA3, and NTRK3 in human cancers.
Human mutation27(10)1060-1(2006 Oct)
Wood LD,Calhoun ES,Silliman N,Ptak J,Szabo S,Powell SM,Riggins GJ,Wang TL,Yan H,Gazdar A,Kern SE,Pennacchio L,Kinzler KW,Vogelstein B,Velculescu VE

The DNA sequence of the human X chromosome.
Nature434(7031)325-37(2005 Mar)
Ross MT,Grafham DV,Coffey AJ,Scherer S,McLay K,Muzny D,Platzer M,Howell GR,Burrows C,Bird CP,Frankish A,Lovell FL,Howe KL,Ashurst JL,Fulton RS,Sudbrak R,Wen G,Jones MC,Hurles ME,Andrews TD,Scott CE,Searle S,Ramser J,Whittaker A,Deadman R,Carter NP,Hunt SE,Chen R,Cree A,Gunaratne P,Havlak P,Hodgson A,Metzker ML,Richards S,Scott G,Steffen D,Sodergren E,Wheeler DA,Worley KC,Ainscough R,Ambrose KD,Ansari-Lari MA,Aradhya S,Ashwell RI,Babbage AK,Bagguley CL,Ballabio A,Banerjee R,Barker GE,Barlow KF,Barrett IP,Bates KN,Beare DM,Beasley H,Beasley O,Beck A,Bethel G,Blechschmidt K,Brady N,Bray-Allen S,Bridgeman AM,Brown AJ,Brown MJ,Bonnin D,Bruford EA,Buhay C,Burch P,Burford D,Burgess J,Burrill W,Burton J,Bye JM,Carder C,Carrel L,Chako J,Chapman JC,Chavez D,Chen E,Chen G,Chen Y,Chen Z,Chinault C,Ciccodicola A,Clark SY,Clarke G,Clee CM,Clegg S,Clerc-Blankenburg K,Clifford K,Cobley V,Cole CG,Conquer JS,Corby N,Connor RE,David R,Davies J,Davis C,Davis J,Delgado O,Deshazo D,Dhami P,Ding Y,Dinh H,Dodsworth S,Draper H,Dugan-Rocha S,Dunham A,Dunn M,Durbin KJ,Dutta I,Eades T,Ellwood M,Emery-Cohen A,Errington H,Evans KL,Faulkner L,Francis F,Frankland J,Fraser AE,Galgoczy P,Gilbert J,Gill R,Gl?ckner G,Gregory SG,Gribble S,Griffiths C,Grocock R,Gu Y,Gwilliam R,Hamilton C,Hart EA,Hawes A,Heath PD,Heitmann K,Hennig S,Hernandez J,Hinzmann B,Ho S,Hoffs M,Howden PJ,Huckle EJ,Hume J,Hunt PJ,Hunt AR,Isherwood J,Jacob L,Johnson D,Jones S,de Jong PJ,Joseph SS,Keenan S,Kelly S,Kershaw JK,Khan Z,Kioschis P,Klages S,Knights AJ,Kosiura A,Kovar-Smith C,Laird GK,Langford C,Lawlor S,Leversha M,Lewis L,Liu W,Lloyd C,Lloyd DM,Loulseged H,Loveland JE,Lovell JD,Lozado R,Lu J,Lyne R,Ma J,Maheshwari M,Matthews LH,McDowall J,McLaren S,McMurray A,Meidl P,Meitinger T,Milne S,Miner G,Mistry SL,Morgan M,Morris S,M?ller I,Mullikin JC,Nguyen N,Nordsiek G,Nyakatura G,O'Dell CN,Okwuonu G,Palmer S,Pandian R,Parker D,Parrish J,Pasternak S,Patel D,Pearce AV,Pearson DM,Pelan SE,Perez L,Porter KM,Ramsey Y,Reichwald K,Rhodes S,Ridler KA,Schlessinger D,Schueler MG,Sehra HK,Shaw-Smith C,Shen H,Sheridan EM,Shownkeen R,Skuce CD,Smith ML,Sotheran EC,Steingruber HE,Steward CA,Storey R,Swann RM,Swarbreck D,Tabor PE,Taudien S,Taylor T,Teague B,Thomas K,Thorpe A,Timms K,Tracey A,Trevanion S,Tromans AC,d'Urso M,Verduzco D,Villasana D,Waldron L,Wall M,Wang Q,Warren J,Warry GL,Wei X,West A,Whitehead SL,Whiteley MN,Wilkinson JE,Willey DL,Williams G,Williams L,Williamson A,Williamson H,Wilming L,Woodmansey RL,Wray PW,Yen J,Zhang J,Zhou J,Zoghbi H,Zorilla S,Buck D,Reinhardt R,Poustka A,Rosenthal A,Lehrach H,Meindl A,Minx PJ,Hillier LW,Willard HF,Wilson RK,Waterston RH,Rice CM,Vaudin M,Coulson A,Nelson DL,Weinstock G,Sulston JE,Durbin R,Hubbard T,Gibbs RA,Beck S,Rogers J,Bentley DR

The kinase homology domain of retinal guanylyl cyclases 1 and 2 specifies the affinity and cooperativity of interaction with guanylyl cyclase activating protein-2.
Biochemistry37(32)11264-71(1998 Aug)
Laura RP,Hurley JB

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GUCY2F cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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