GUCA2B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GUCA2B
Entrez Gene ID	2981
Full Name	guanylate cyclase activator 2B
Synonyms	GCAP-II,UGN
General protein information	Preferred Names guanylate cyclase activator 2B Names guanylate cyclase activator 2B prepro-uroguanylin uroguanylin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.2
Summary	This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products, including uroguanylin, a member of the guanylin family of peptides and an endogenous ligand of the guanylate cyclase-C receptor. Binding of this peptide to its cognate receptor stimulates an increase in cyclic GMP and may regulate salt and water homeostasis in the intestine and kidneys. [provided by RefSeq, Nov 2015].
Disorder MIM:	601271

mRNA and Protein(s)

mRNA	Protein	Name
NM_007102.2	NP_009033.1	guanylate cyclase activator 2B preproprotein
NM_007102.3	NP_009033.1	guanylate cyclase activator 2B preproprotein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GUCA2B	NP_001192745.1
Mus musculus (house mouse)	Guca2b	NP_032217.2
Pan troglodytes (chimpanzee)	GUCA2B	XP_524686.2
	GUCA2B	XP_001087987.1
Canis lupus familiaris (dog)	GUCA2B	XP_003431998.1
Homo sapiens (human)	GUCA2B	NP_009033.1
Rattus norvegicus (Norway rat)	Guca2b	NP_071620.1

Gene Ontology
Bibliography

Related articles in PubMed

Guanylin and uroguanylin mRNA expression is increased following Roux-en-Y gastric bypass, but guanylins do not play a significant role in body weight regulation and glycemic control.
Fernandez-Cachon ML, Pedersen SL, Rigbolt KT, Zhang C, Fabricius K, Hansen HH, Elster L, Fink LN, Sch?fer M, Rhee NA, Langholz E, Wandall E, Friis SU, Vilmann P, Kristiansen VB, Schmidt C, Schreiter K, Breitschopf K, H?bschle T, Jorsal T, Vilsb?ll T, Schmidt T, Theis S, Knop FK, Larsen PJ, Jelsing J
Peptides10132-43(2017 Dec)

A Pilot Study Measuring The Novel Satiety Hormone, Pro-Uroguanylin, In Adolescents With And Without Obesity.
Di Guglielmo MD, Tonb D, He Z, Adeyemi A, van Golen KL
Journal of pediatric gastroenterology and nutrition(2017 Nov)

Immunohistochemical Staining for Uroguanylin, a Satiety Hormone, Is Decreased in Intestinal Tissue Specimens From Female Adolescents With Obesity.
Di Guglielmo MD, Perdue L, Adeyemi A, van Golen KL, Corao DU
Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society1093526617722912(2017 Jan)

Structural impact analysis of missense SNPs present in the uroguanylin gene by long-term molecular dynamics simulations.
Marcolino AC, Porto WF, Pires ?S, Franco OL, Alencar SA
Journal of theoretical biology4109-17(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The simulations suggested that all missense SNPs considered as convergent deleterious caused some kind of structural change to the uroguanylin peptide. Additionally, four of these SNPs were also shown to cause modifications in peptide flexibility, possibly resulting in functional changes.
Title: Structural impact analysis of missense SNPs present in the uroguanylin gene by long-term molecular dynamics simulations.

The specific cellular distribution of both GN and UGN differs between duodenum and colon and between human and rat intestines.
Title: Cellular localization of guanylin and uroguanylin mRNAs in human and rat duodenal and colonic mucosa.

The occurrences of the C-A haplotype (rs883062-rs1047047) and the C-A-G haplotype (rs883062-rs1047047-rs2297566) were significantly higher in the essential hypertension group than in the controls.
Title: Haplotype-based case-control study of the association between the guanylate cyclase activator 2B (GUCA2B, Uroguanylin) gene and essential hypertension.

Observational study of gene-disease association. (HuGE Navigator)
Title: [Association between uroguanylin G-247A polymorphism and blood pressure/fluid and electrolytes homeostasis].

Uroguanylin concentrations are increased in patients with chronic renal failure, nephrotic syndrome, or those on dialysis.
Title: Guanylin and uroguanylin regulate electrolyte transport in isolated human cortical collecting ducts.

The following GUCA2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUCA2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05921	NM_007102.3 Latest version!	Homo sapiens guanylate cyclase activator 2B (GUCA2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu05921	NM_007102.2	Homo sapiens guanylate cyclase activator 2B (GUCA2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GUCA2B ( NM_007102.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_009033.1 Homo sapiens guanylate cyclase activator 2B (GUCA2B), mRNA.

Price & Availability↑

CloneID	OHu05921
Clone ID Related Accession (Same CDS sequence)	NM_007102.2 , NM_007102.3
Accession Version	NM_007102.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	guanylate cyclase activator 2B preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U34279.1, BC093781.1 and Z50753.1. On Mar 12, 2008 this sequence version replaced NM_007102.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U34279.1, ERR279837.3448.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTGCA GGGCTGCATC AGGGCTCCTG CCAGGAGTGG CCGTGGTCCT CCTGCTGCTG 
CTGCAGAGCA CACAGTCAGT CTACATCCAG TACCAAGGCT TCCGGGTCCA GCTGGAATCC 
ATGAAGAAGC TGAGTGACCT GGAGGCACAG TGGGCACCCA GCCCCCGCCT GCAGGCCCAG 
AGCCTCCTGC CCGCCGTGTG CCACCACCCT GCTCTGCCTC AGGACCTTCA GCCTGTCTGC 
GCCTCGCAGG AGGCTTCCAG CATCTTCAAG ACCCTGAGGA CCATCGCTAA CGACGACTGT 
GAGCTGTGTG TGAACGTTGC GTGTACCGGC TGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009033.1
CDS	31..369
Translation	MGCRAASGLLPGVAVVLLLLLQSTQSVYIQYQGFRVQLESMKKLSDLEAQWAPSPRLQAQSLLPAVCHHPALPQDLQPVCASQEASSIFKTLRTIANDDCELCVNVACTGCL

Target ORF information:

RefSeq Version	NM_007102.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase activator 2B (GUCA2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007102.2

ORF Insert Sequence:

ATGGGCTGCA GGGCTGCATC AGGGCTCCTG CCAGGAGTGG CCGTGGTCCT CCTGCTGCTG 
CTGCAGAGCA CACAGTCAGT CTACATCCAG TACCAAGGCT TCCGGGTCCA GCTGGAATCC 
ATGAAGAAGC TGAGTGACCT GGAGGCACAG TGGGCACCCA GCCCCCGCCT GCAGGCCCAG 
AGCCTCCTGC CCGCCGTGTG CCACCACCCT GCTCTGCCTC AGGACCTTCA GCCTGTCTGC 
GCCTCGCAGG AGGCTTCCAG CATCTTCAAG ACCCTGAGGA CCATCGCTAA CGACGACTGT 
GAGCTGTGTG TGAACGTTGC GTGTACCGGC TGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCA2B ( NM_007102.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_009033.1 Homo sapiens guanylate cyclase activator 2B (GUCA2B), mRNA.

Price & Availability↑

CloneID	OHu05921
Clone ID Related Accession (Same CDS sequence)	NM_007102.2 , NM_007102.3
Accession Version	NM_007102.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	guanylate cyclase activator 2B preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114492.6, U34279.1, BC093781.1 and Z50753.1. On Nov 22, 2018 this sequence version replaced NM_007102.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279826.2155.1, U34279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000372581.2/ ENSP00000361662.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCTGCA GGGCTGCATC AGGGCTCCTG CCAGGAGTGG CCGTGGTCCT CCTGCTGCTG 
CTGCAGAGCA CACAGTCAGT CTACATCCAG TACCAAGGCT TCCGGGTCCA GCTGGAATCC 
ATGAAGAAGC TGAGTGACCT GGAGGCACAG TGGGCACCCA GCCCCCGCCT GCAGGCCCAG 
AGCCTCCTGC CCGCCGTGTG CCACCACCCT GCTCTGCCTC AGGACCTTCA GCCTGTCTGC 
GCCTCGCAGG AGGCTTCCAG CATCTTCAAG ACCCTGAGGA CCATCGCTAA CGACGACTGT 
GAGCTGTGTG TGAACGTTGC GTGTACCGGC TGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009033.1
CDS	42..380
Translation	MGCRAASGLLPGVAVVLLLLLQSTQSVYIQYQGFRVQLESMKKLSDLEAQWAPSPRLQAQSLLPAVCHHPALPQDLQPVCASQEASSIFKTLRTIANDDCELCVNVACTGCL

Target ORF information:

RefSeq Version	NM_007102.3
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase activator 2B (GUCA2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007102.3

ORF Insert Sequence:

ATGGGCTGCA GGGCTGCATC AGGGCTCCTG CCAGGAGTGG CCGTGGTCCT CCTGCTGCTG 
CTGCAGAGCA CACAGTCAGT CTACATCCAG TACCAAGGCT TCCGGGTCCA GCTGGAATCC 
ATGAAGAAGC TGAGTGACCT GGAGGCACAG TGGGCACCCA GCCCCCGCCT GCAGGCCCAG 
AGCCTCCTGC CCGCCGTGTG CCACCACCCT GCTCTGCCTC AGGACCTTCA GCCTGTCTGC 
GCCTCGCAGG AGGCTTCCAG CATCTTCAAG ACCCTGAGGA CCATCGCTAA CGACGACTGT 
GAGCTGTGTG TGAACGTTGC GTGTACCGGC TGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Guanylin and uroguanylin mRNA expression is increased following Roux-en-Y gastric bypass, but guanylins do not play a significant role in body weight regulation and glycemic control.
Peptides10132-43(2017 Dec)
Fernandez-Cachon ML,Pedersen SL,Rigbolt KT,Zhang C,Fabricius K,Hansen HH,Elster L,Fink LN,Sch?fer M,Rhee NA,Langholz E,Wandall E,Friis SU,Vilmann P,Kristiansen VB,Schmidt C,Schreiter K,Breitschopf K,H?bschle T,Jorsal T,Vilsb?ll T,Schmidt T,Theis S,Knop FK,Larsen PJ,Jelsing J

A Pilot Study Measuring The Novel Satiety Hormone, Pro-Uroguanylin, In Adolescents With And Without Obesity.
Journal of pediatric gastroenterology and nutrition(2017 Nov)
Di Guglielmo MD,Tonb D,He Z,Adeyemi A,van Golen KL

Immunohistochemical Staining for Uroguanylin, a Satiety Hormone, Is Decreased in Intestinal Tissue Specimens From Female Adolescents With Obesity.
Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society1093526617722912(2017 Jan)
Di Guglielmo MD,Perdue L,Adeyemi A,van Golen KL,Corao DU

Structural impact analysis of missense SNPs present in the uroguanylin gene by long-term molecular dynamics simulations.
Journal of theoretical biology4109-17(2016 Dec)
Marcolino AC,Porto WF,Pires ?S,Franco OL,Alencar SA

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GUCA2B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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