GUCA1B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GUCA1B
Entrez Gene ID	2979
Full Name	guanylate cyclase activator 1B
Synonyms	GCAP2,GUCA2,RP48
General protein information	Preferred Names guanylate cyclase activator 1B Names guanylyl cyclase-activating protein 2 GCAP 2 guanylate cyclase activator 1B (retina) guanylate cyclase-activating protein, photoreceptor 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.1
Summary	The protein encoded by this gene is a calcium-binding protein that activates photoreceptor guanylate cyclases. This gene may have arisen due to a gene duplication event since there is a highly similar gene clustered with it on chromosome 6. Mutations in this gene can cause a form of retinitis pigmentosa. [provided by RefSeq, Nov 2009].
Disorder MIM:	602275
Disorder Html:	Retinitis pigmentosa 48, 613827 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002098.6	NP_002089.4	guanylyl cyclase-activating protein 2
NM_002098.5	NP_002089.4	guanylyl cyclase-activating protein 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GUCA1B	XP_527386.2
Gallus gallus (chicken)	GUCA1B	NP_001007882.1
Canis lupus familiaris (dog)	GUCA1B	XP_538922.1
Danio rerio (zebrafish)	guca1b	NP_571946.1
Xenopus tropicalis (tropical clawed frog)	LOC100489509	XP_002933553.1
Homo sapiens (human)	GUCA1B	NP_002089.4
Bos taurus (cattle)	GUCA1B	NP_777211.1
Mus musculus (house mouse)	Guca1b	NP_666191.1
Rattus norvegicus (Norway rat)	Guca1b	NP_001101668.1
	GUCA1B	XP_001086988.1

Gene Ontology
Bibliography

Related articles in PubMed

Label-free quantification of calcium-sensor targeting to photoreceptor guanylate cyclase and rhodopsin kinase by backscattering interferometry.
Sulmann S, Kussrow A, Bornhop DJ, Koch KW
Scientific reports745515(2017 Mar)

Dimerization Domain of Retinal Membrane Guanylyl Cyclase 1 (RetGC1) Is an Essential Part of Guanylyl Cyclase-activating Protein (GCAP) Binding Interface.
Peshenko IV, Olshevskaya EV, Dizhoor AM
The Journal of biological chemistry290(32)19584-96(2015 Aug)

Evaluating the role of retinal membrane guanylyl cyclase 1 (RetGC1) domains in binding guanylyl cyclase-activating proteins (GCAPs).
Peshenko IV, Olshevskaya EV, Dizhoor AM
The Journal of biological chemistry290(11)6913-24(2015 Mar)

Mutation screening of the GUCA1B gene in patients with autosomal dominant cone and cone rod dystrophy.
Kitiratschky VB, Gl?ckner CJ, Kohl S
Ophthalmic genetics32(3)151-5(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dimerization domain of RETGC1 is an essential part of GCAP1 and GCAP2 binding interface.
Title: Dimerization Domain of Retinal Membrane Guanylyl Cyclase 1 (RetGC1) Is an Essential Part of Guanylyl Cyclase-activating Protein (GCAP) Binding Interface.

The GCAP1 and GCAP2 binding site(s) overlaps within the kinase homology and/or dimerization domains of retinal GC1.
Title: Evaluating the role of retinal membrane guanylyl cyclase 1 (RetGC1) domains in binding guanylyl cyclase-activating proteins (GCAPs).

The absence of clearly pathogenic mutations in the selected patient group suggests that the GUCA1B gene is a minor cause for retinal degenerations in Europeans or North-Americans.
Title: Mutation screening of the GUCA1B gene in patients with autosomal dominant cone and cone rod dystrophy.

Observational study of genotype prevalence and gene-disease association. (HuGE Navigator)
Title: A novel mutation (I143NT) in guanylate cyclase-activating protein 1 (GCAP1) associated with autosomal dominant cone degeneration.

Observational study of genotype prevalence. (HuGE Navigator)
Title: [Analysis of GUCA1B,GNGT1 and RGS9 genes in patients with retinitis pigmentosa].

The following GUCA1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUCA1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23111	NM_002098.6 Latest version!	Homo sapiens guanylate cyclase activator 1B (GUCA1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
View Old Accession Versions >>
OHu23111	NM_002098.5	Homo sapiens guanylate cyclase activator 1B (GUCA1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GUCA1B ( NM_002098.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_002089.4 Homo sapiens guanylate cyclase activator 1B (GUCA1B), mRNA.

Price & Availability↑

CloneID	OHu23111
Clone ID Related Accession (Same CDS sequence)	NM_002098.6 , NM_002098.5
Accession Version	NM_002098.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	guanylyl cyclase-activating protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL096814.26. This sequence is a reference standard in the RefSeqGene project. On Nov 29, 2018 this sequence version replaced NM_002098.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537393.1, BQ636025.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved MANE Ensembl match :: ENST00000230361.4/ ENSP00000230361.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGCAGG AGTTTAGCTG GGAGGAGGCG GAGGCAGCTG GCGAGATAGA TGTGGCGGAG 
CTCCAGGAGT GGTACAAGAA GTTTGTGATG GAGTGCCCCA GCGGCACACT CTTTATGCAT 
GAGTTTAAGC GCTTCTTCAA GGTCACAGAC GATGAGGAGG CCTCCCAGTA TGTAGAGGGC 
ATGTTCCGAG CCTTCGACAA GAATGGGGAC AACACCATCG ACTTCCTGGA GTACGTGGCA 
GCTCTGAATC TCGTGCTGAG GGGCACCCTG GAGCACAAGC TGAAGTGGAC ATTCAAGATC 
TATGATAAGG ATGGCAATGG CTGCATCGAC CGCCTGGAGC TACTCAACAT TGTGGAGGGA 
ATTTACCAGC TGAAGAAAGC CTGCCGGCGA GAGCTACAAA CTGAGCAAGG CCAGCTGCTC 
ACACCCGAGG AGGTCGTGGA CAGGATCTTC CTCCTGGTGG ATGAGAATGG AGATGGCCAG 
CTGTCTCTGA ACGAGTTTGT TGAAGGTGCC CGTCGGGACA AGTGGGTGAT GAAGATGCTG 
CAGATGGACA TGAATCCCAG CAGCTGGCTC GCTCAGCAGA GACGGAAAAG TGCCATGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002089.4
CDS	137..739
Translation	MGQEFSWEEAEAAGEIDVAELQEWYKKFVMECPSGTLFMHEFKRFFKVTDDEEASQYVEGMFRAFDKNGDNTIDFLEYVAALNLVLRGTLEHKLKWTFKIYDKDGNGCIDRLELLNIVEGIYQLKKACRRELQTEQGQLLTPEEVVDRIFLLVDENGDGQLSLNEFVEGARRDKWVMKMLQMDMNPSSWLAQQRRKSAMF

Target ORF information:

RefSeq Version	NM_002098.6
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase activator 1B (GUCA1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002098.6

ORF Insert Sequence:

ATGGGGCAGG AGTTTAGCTG GGAGGAGGCG GAGGCAGCTG GCGAGATAGA TGTGGCGGAG 
CTCCAGGAGT GGTACAAGAA GTTTGTGATG GAGTGCCCCA GCGGCACACT CTTTATGCAT 
GAGTTTAAGC GCTTCTTCAA GGTCACAGAC GATGAGGAGG CCTCCCAGTA TGTAGAGGGC 
ATGTTCCGAG CCTTCGACAA GAATGGGGAC AACACCATCG ACTTCCTGGA GTACGTGGCA 
GCTCTGAATC TCGTGCTGAG GGGCACCCTG GAGCACAAGC TGAAGTGGAC ATTCAAGATC 
TATGATAAGG ATGGCAATGG CTGCATCGAC CGCCTGGAGC TACTCAACAT TGTGGAGGGA 
ATTTACCAGC TGAAGAAAGC CTGCCGGCGA GAGCTACAAA CTGAGCAAGG CCAGCTGCTC 
ACACCCGAGG AGGTCGTGGA CAGGATCTTC CTCCTGGTGG ATGAGAATGG AGATGGCCAG 
CTGTCTCTGA ACGAGTTTGT TGAAGGTGCC CGTCGGGACA AGTGGGTGAT GAAGATGCTG 
CAGATGGACA TGAATCCCAG CAGCTGGCTC GCTCAGCAGA GACGGAAAAG TGCCATGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCA1B ( NM_002098.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002089.4 Homo sapiens guanylate cyclase activator 1B (GUCA1B), mRNA.

Price & Availability↑

CloneID	OHu23111
Clone ID Related Accession (Same CDS sequence)	NM_002098.6 , NM_002098.5
Accession Version	NM_002098.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	guanylyl cyclase-activating protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX537393.1, AL096814.26 and BQ636025.1. This sequence is a reference standard in the RefSeqGene project. On Dec 1, 2009 this sequence version replaced NM_002098.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537393.1, BQ636025.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGG AGTTTAGCTG GGAGGAGGCG GAGGCAGCTG GCGAGATAGA TGTGGCGGAG 
CTCCAGGAGT GGTACAAGAA GTTTGTGATG GAGTGCCCCA GCGGCACACT CTTTATGCAT 
GAGTTTAAGC GCTTCTTCAA GGTCACAGAC GATGAGGAGG CCTCCCAGTA TGTAGAGGGC 
ATGTTCCGAG CCTTCGACAA GAATGGGGAC AACACCATCG ACTTCCTGGA GTACGTGGCA 
GCTCTGAATC TCGTGCTGAG GGGCACCCTG GAGCACAAGC TGAAGTGGAC ATTCAAGATC 
TATGATAAGG ATGGCAATGG CTGCATCGAC CGCCTGGAGC TACTCAACAT TGTGGAGGGA 
ATTTACCAGC TGAAGAAAGC CTGCCGGCGA GAGCTACAAA CTGAGCAAGG CCAGCTGCTC 
ACACCCGAGG AGGTCGTGGA CAGGATCTTC CTCCTGGTGG ATGAGAATGG AGATGGCCAG 
CTGTCTCTGA ACGAGTTTGT TGAAGGTGCC CGTCGGGACA AGTGGGTGAT GAAGATGCTG 
CAGATGGACA TGAATCCCAG CAGCTGGCTC GCTCAGCAGA GACGGAAAAG TGCCATGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002089.4
CDS	137..739
Translation	MGQEFSWEEAEAAGEIDVAELQEWYKKFVMECPSGTLFMHEFKRFFKVTDDEEASQYVEGMFRAFDKNGDNTIDFLEYVAALNLVLRGTLEHKLKWTFKIYDKDGNGCIDRLELLNIVEGIYQLKKACRRELQTEQGQLLTPEEVVDRIFLLVDENGDGQLSLNEFVEGARRDKWVMKMLQMDMNPSSWLAQQRRKSAMF

Target ORF information:

RefSeq Version	NM_002098.5
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase activator 1B (GUCA1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002098.5

ORF Insert Sequence:

ATGGGGCAGG AGTTTAGCTG GGAGGAGGCG GAGGCAGCTG GCGAGATAGA TGTGGCGGAG 
CTCCAGGAGT GGTACAAGAA GTTTGTGATG GAGTGCCCCA GCGGCACACT CTTTATGCAT 
GAGTTTAAGC GCTTCTTCAA GGTCACAGAC GATGAGGAGG CCTCCCAGTA TGTAGAGGGC 
ATGTTCCGAG CCTTCGACAA GAATGGGGAC AACACCATCG ACTTCCTGGA GTACGTGGCA 
GCTCTGAATC TCGTGCTGAG GGGCACCCTG GAGCACAAGC TGAAGTGGAC ATTCAAGATC 
TATGATAAGG ATGGCAATGG CTGCATCGAC CGCCTGGAGC TACTCAACAT TGTGGAGGGA 
ATTTACCAGC TGAAGAAAGC CTGCCGGCGA GAGCTACAAA CTGAGCAAGG CCAGCTGCTC 
ACACCCGAGG AGGTCGTGGA CAGGATCTTC CTCCTGGTGG ATGAGAATGG AGATGGCCAG 
CTGTCTCTGA ACGAGTTTGT TGAAGGTGCC CGTCGGGACA AGTGGGTGAT GAAGATGCTG 
CAGATGGACA TGAATCCCAG CAGCTGGCTC GCTCAGCAGA GACGGAAAAG TGCCATGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Label-free quantification of calcium-sensor targeting to photoreceptor guanylate cyclase and rhodopsin kinase by backscattering interferometry.
Scientific reports745515(2017 Mar)
Sulmann S,Kussrow A,Bornhop DJ,Koch KW

Dimerization Domain of Retinal Membrane Guanylyl Cyclase 1 (RetGC1) Is an Essential Part of Guanylyl Cyclase-activating Protein (GCAP) Binding Interface.
The Journal of biological chemistry290(32)19584-96(2015 Aug)
Peshenko IV,Olshevskaya EV,Dizhoor AM

Evaluating the role of retinal membrane guanylyl cyclase 1 (RetGC1) domains in binding guanylyl cyclase-activating proteins (GCAPs).
The Journal of biological chemistry290(11)6913-24(2015 Mar)
Peshenko IV,Olshevskaya EV,Dizhoor AM

Mutation screening of the GUCA1B gene in patients with autosomal dominant cone and cone rod dystrophy.
Ophthalmic genetics32(3)151-5(2011 Sep)
Kitiratschky VB,Gl?ckner CJ,Kohl S

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GUCA1B cDNA ORF clone, Homo sapiens(human)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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