GTF2F1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GTF2F1
Entrez Gene ID	2962
Full Name	general transcription factor IIF subunit 1
Synonyms	BTF4,RAP74,TF2F1,TFIIF
General protein information	Preferred Names general transcription factor IIF subunit 1 Names general transcription factor IIF subunit 1 TFIIF-alpha general transcription factor IIF 74 kDa subunit general transcription factor IIF, polypeptide 1, 74kDa transcription initiation factor IIF subunit alpha transcription initiation factor RAP74
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.3
Disorder MIM:	189968

mRNA and Protein(s)

mRNA	Protein	Name
NM_002096.3	NP_002087.2	general transcription factor IIF subunit 1
XM_024451475.1	XP_024307243.1	general transcription factor IIF subunit 1 isoform X1
NM_002096.2	NP_002087.2	general transcription factor IIF subunit 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GTF2F1	NP_001015527.1
Danio rerio (zebrafish)	gtf2f1	NP_956023.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002779	XP_312144.5
Xenopus tropicalis (tropical clawed frog)	gtf2f1	NP_001116271.1
Homo sapiens (human)	GTF2F1	NP_002087.2
Pan troglodytes (chimpanzee)	GTF2F1	XP_512314.2
	GTF2F1	XP_001087325.1
Rattus norvegicus (Norway rat)	Gtf2f1	NP_001007712.1
Canis lupus familiaris (dog)	GTF2F1	XP_005633036.1
Mus musculus (house mouse)	Gtf2f1	NP_598562.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of Androgen Receptor Activity by Transient Interactions of Its Transactivation Domain with General Transcription Regulators.
De Mol E, Szulc E, Di Sanza C, Mart?nez-Crist?bal P, Bertoncini CW, Fenwick RB, Frigol?-Vivas M, Mas?n M, Hunter I, Buz?n V, Brun-Heath I, Garc?a J, De Fabritiis G, Est?banez-Perpi?? E, McEwan IJ, Nebreda ?R, Salvatella X
Structure (London, England : 1993)26(1)145-152.e3(2018 Jan)

Near-atomic resolution visualization of human transcription promoter opening.
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature533(7603)359-65(2016 05)

A new paradigm for transcription factor TFIIB functionality.
Gelev V, Zabolotny JM, Lange M, Hiromura M, Yoo SW, Orlando JS, Kushnir A, Horikoshi N, Paquet E, Bachvarov D, Schaffer PA, Usheva A
Scientific reports43664(2014 Jan)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that Mediator structural shifts induced by activator binding help stably orient pol II prior to transcription initiation within the human mediator-RNA polymerase II-TFIIF assembly.
Title: Activator-mediator binding stabilizes RNA polymerase II orientation within the human mediator-RNA polymerase II-TFIIF assembly.

Gdown1 competes with TFIIF for binding to the RPB1 and RPB5 subunits of Pol II, thereby inhibiting an essential function of TFIIF in preinitiation complex assembly.
Title: Transcriptional regulation by Pol II(G) involving mediator and competitive interactions of Gdown1 and TFIIF with Pol II.

Data show that TFIIF has an important role in stabilizing TFIIB within the PIC and after transcription initiates.
Title: Transcription factor TFIIF is not required for initiation by RNA polymerase II, but it is essential to stabilize transcription factor TFIIB in early elongation complexes.

NMR and thermodynamic studies further elucidate the complex molecular mechanism by which TFIIF and FCP1 cooperate for RNAPII recycling.
Title: NMR structure of a complex formed by the carboxyl-terminal domain of human RAP74 and a phosphorylated peptide from the central domain of the FCP1 phosphatase.

Observational study of gene-disease association. (HuGE Navigator)
Title: Evaluating new candidate SNPs as low penetrance risk factors in sporadic breast cancer: a two-stage Spanish case-control study.

The following GTF2F1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GTF2F1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03831	NM_002096.3 Latest version!	Homo sapiens general transcription factor IIF subunit 1 (GTF2F1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu104663	XM_024451475.1 Latest version!	Homo sapiens general transcription factor IIF subunit 1 (GTF2F1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu03831	NM_002096.2	Homo sapiens general transcription factor IIF subunit 1 (GTF2F1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GTF2F1 ( NM_002096.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002087.2 Homo sapiens general transcription factor IIF subunit 1 (GTF2F1), mRNA.

Price & Availability↑

CloneID	OHu03831
Clone ID Related Accession (Same CDS sequence)	NM_002096.3 , NM_002096.2
Accession Version	NM_002096.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	general transcription factor IIF subunit 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from X64037.1, BC013007.2 and X64002.1. On Nov 23, 2018 this sequence version replaced NM_002096.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X64002.1, SRR1660807.46796.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000394456.10/ ENSP00000377969.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCCC TAGGCCCTAG CAGCCAGAAT GTCACTGAAT ACGTCGTTCG AGTTCCTAAG 
AATACAACCA AAAAATATAA CATCATGGCT TTTAATGCAG CCGACAAAGT CAACTTTGCT 
ACGTGGAATC AGGCTCGGCT GGAGCGGGAC TTGAGCAACA AGAAAATCTA CCAAGAGGAG 
GAGATGCCCG AATCGGGCGC GGGCAGTGAG TTCAACCGCA AGCTTCGGGA GGAGGCTCGG 
AGGAAGAAGT ACGGCATCGT CCTCAAGGAG TTCCGGCCCG AGGACCAGCC CTGGCTGCTC 
CGGGTCAACG GCAAATCAGG CAGGAAGTTC AAGGGCATCA AGAAGGGAGG CGTAACAGAG 
AACACGTCCT ACTACATCTT CACCCAGTGC CCCGACGGGG CCTTCGAGGC CTTCCCCGTG 
CACAACTGGT ACAATTTCAC ACCGCTGGCC CGGCATCGCA CGCTCACTGC CGAGGAGGCC 
GAGGAGGAGT GGGAGAGGAG GAACAAGGTG CTGAACCACT TCAGCATCAT GCAGCAGCGG 
CGGCTCAAGG ATCAGGACCA GGACGAGGAT GAGGAGGAGA AGGAGAAACG TGGCCGCAGG 
AAGGCGAGCG AGCTGCGCAT CCACGACCTG GAGGACGACC TGGAGATGTC GTCCGATGCC 
AGTGATGCCA GTGGTGAGGA GGGGGGCAGA GTCCCCAAGG CCAAGAAGAA GGCGCCGCTG 
GCCAAGGGCG GCAGGAAAAA GAAGAAGAAG AAGGGTTCAG ACGACGAGGC CTTCGAGGAC 
AGCGATGATG GGGACTTCGA GGGCCAAGAG GTGGACTACA TGTCAGACGG CTCCAGTAGC 
TCCCAAGAAG AGCCTGAGAG CAAGGCCAAG GCGCCGCAGC AGGAGGAGGG GCCCAAGGGT 
GTCGATGAGC AGAGCGACAG TAGTGAGGAG AGTGAGGAGG AGAAGCCGCC TGAGGAGGAC 
AAGGAGGAGG AGGAGGAGAA GAAGGCACCC ACCCCGCAGG AGAAGAAGCG CAGGAAAGAC 
AGCAGCGAGG AGTCGGACAG CTCAGAGGAG AGCGACATTG ACAGCGAGGC CTCCTCAGCC 
CTCTTCATGG CGAAGAAGAA GACGCCACCC AAGAGAGAGC GGAAGCCGTC GGGAGGGAGC 
TCAAGGGGCA ACAGCCGCCC AGGCACGCCC AGCGCAGAGG GTGGCAGCAC CTCCTCCACC 
CTGCGGGCGG CTGCCAGCAA ACTCGAGCAA GGGAAGCGGG TGAGCGAGAT GCCTGCAGCC 
AAGCGGTTGC GGCTGGACAC GGGACCCCAG AGCCTGTCTG GGAAGTCGAC ACCCCAGCCA 
CCATCAGGCA AGACAACACC CAACAGCGGC GACGTGCAGG TGACTGAGGA TGCCGTGCGC 
CGCTACCTGA CACGGAAGCC CATGACCACT AAGGACCTGC TGAAAAAGTT CCAGACCAAG 
AAGACAGGGC TGAGCAGCGA GCAGACAGTG AACGTGTTGG CCCAGATCCT CAAGCGACTC 
AACCCCGAGC GCAAGATGAT CAACGACAAA ATGCACTTCT CCCTCAAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002087.2
CDS	170..1723
Translation	MAALGPSSQNVTEYVVRVPKNTTKKYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEEEMPESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTSYYIFTQCPDGAFEAFPVHNWYNFTPLARHRTLTAEEAEEEWERRNKVLNHFSIMQQRRLKDQDQDEDEEEKEKRGRRKASELRIHDLEDDLEMSSDASDASGEEGGRVPKAKKKAPLAKGGRKKKKKKGSDDEAFEDSDDGDFEGQEVDYMSDGSSSSQEEPESKAKAPQQEEGPKGVDEQSDSSEESEEEKPPEEDKEEEEEKKAPTPQEKKRRKDSSEESDSSEESDIDSEASSALFMAKKKTPPKRERKPSGGSSRGNSRPGTPSAEGGSTSSTLRAAASKLEQGKRVSEMPAAKRLRLDTGPQSLSGKSTPQPPSGKTTPNSGDVQVTEDAVRRYLTRKPMTTKDLLKKFQTKKTGLSSEQTVNVLAQILKRLNPERKMINDKMHFSLKE

Target ORF information:

RefSeq Version	NM_002096.3
Organism	Homo sapiens(human)
Definition	Homo sapiens general transcription factor IIF subunit 1 (GTF2F1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002096.3

ORF Insert Sequence:

ATGGCGGCCC TAGGCCCTAG CAGCCAGAAT GTCACTGAAT ACGTCGTTCG AGTTCCTAAG 
AATACAACCA AAAAATATAA CATCATGGCT TTTAATGCAG CCGACAAAGT CAACTTTGCT 
ACGTGGAATC AGGCTCGGCT GGAGCGGGAC TTGAGCAACA AGAAAATCTA CCAAGAGGAG 
GAGATGCCCG AATCGGGCGC GGGCAGTGAG TTCAACCGCA AGCTTCGGGA GGAGGCTCGG 
AGGAAGAAGT ACGGCATCGT CCTCAAGGAG TTCCGGCCCG AGGACCAGCC CTGGCTGCTC 
CGGGTCAACG GCAAATCAGG CAGGAAGTTC AAGGGCATCA AGAAGGGAGG CGTAACAGAG 
AACACGTCCT ACTACATCTT CACCCAGTGC CCCGACGGGG CCTTCGAGGC CTTCCCCGTG 
CACAACTGGT ACAATTTCAC ACCGCTGGCC CGGCATCGCA CGCTCACTGC CGAGGAGGCC 
GAGGAGGAGT GGGAGAGGAG GAACAAGGTG CTGAACCACT TCAGCATCAT GCAGCAGCGG 
CGGCTCAAGG ATCAGGACCA GGACGAGGAT GAGGAGGAGA AGGAGAAACG TGGCCGCAGG 
AAGGCGAGCG AGCTGCGCAT CCACGACCTG GAGGACGACC TGGAGATGTC GTCCGATGCC 
AGTGATGCCA GTGGTGAGGA GGGGGGCAGA GTCCCCAAGG CCAAGAAGAA GGCGCCGCTG 
GCCAAGGGCG GCAGGAAAAA GAAGAAGAAG AAGGGTTCAG ACGACGAGGC CTTCGAGGAC 
AGCGATGATG GGGACTTCGA GGGCCAAGAG GTGGACTACA TGTCAGACGG CTCCAGTAGC 
TCCCAAGAAG AGCCTGAGAG CAAGGCCAAG GCGCCGCAGC AGGAGGAGGG GCCCAAGGGT 
GTCGATGAGC AGAGCGACAG TAGTGAGGAG AGTGAGGAGG AGAAGCCGCC TGAGGAGGAC 
AAGGAGGAGG AGGAGGAGAA GAAGGCACCC ACCCCGCAGG AGAAGAAGCG CAGGAAAGAC 
AGCAGCGAGG AGTCGGACAG CTCAGAGGAG AGCGACATTG ACAGCGAGGC CTCCTCAGCC 
CTCTTCATGG CGAAGAAGAA GACGCCACCC AAGAGAGAGC GGAAGCCGTC GGGAGGGAGC 
TCAAGGGGCA ACAGCCGCCC AGGCACGCCC AGCGCAGAGG GTGGCAGCAC CTCCTCCACC 
CTGCGGGCGG CTGCCAGCAA ACTCGAGCAA GGGAAGCGGG TGAGCGAGAT GCCTGCAGCC 
AAGCGGTTGC GGCTGGACAC GGGACCCCAG AGCCTGTCTG GGAAGTCGAC ACCCCAGCCA 
CCATCAGGCA AGACAACACC CAACAGCGGC GACGTGCAGG TGACTGAGGA TGCCGTGCGC 
CGCTACCTGA CACGGAAGCC CATGACCACT AAGGACCTGC TGAAAAAGTT CCAGACCAAG 
AAGACAGGGC TGAGCAGCGA GCAGACAGTG AACGTGTTGG CCCAGATCCT CAAGCGACTC 
AACCCCGAGC GCAAGATGAT CAACGACAAA ATGCACTTCT CCCTCAAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTF2F1 ( NM_002096.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002087.2 Homo sapiens general transcription factor IIF subunit 1 (GTF2F1), mRNA.

Price & Availability↑

CloneID	OHu03831
Clone ID Related Accession (Same CDS sequence)	NM_002096.3 , NM_002096.2
Accession Version	NM_002096.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	general transcription factor IIF subunit 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AA323384.1, X64037.1, BC013007.2 and X64002.1. On Aug 17, 2007 this sequence version replaced NM_002096.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013007.2, SRR1803617.195954.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCCC TAGGCCCTAG CAGCCAGAAT GTCACTGAAT ACGTCGTTCG AGTTCCTAAG 
AATACAACCA AAAAATATAA CATCATGGCT TTTAATGCAG CCGACAAAGT CAACTTTGCT 
ACGTGGAATC AGGCTCGGCT GGAGCGGGAC TTGAGCAACA AGAAAATCTA CCAAGAGGAG 
GAGATGCCCG AATCGGGCGC GGGCAGTGAG TTCAACCGCA AGCTTCGGGA GGAGGCTCGG 
AGGAAGAAGT ACGGCATCGT CCTCAAGGAG TTCCGGCCCG AGGACCAGCC CTGGCTGCTC 
CGGGTCAACG GCAAATCAGG CAGGAAGTTC AAGGGCATCA AGAAGGGAGG CGTAACAGAG 
AACACGTCCT ACTACATCTT CACCCAGTGC CCCGACGGGG CCTTCGAGGC CTTCCCCGTG 
CACAACTGGT ACAATTTCAC ACCGCTGGCC CGGCATCGCA CGCTCACTGC CGAGGAGGCC 
GAGGAGGAGT GGGAGAGGAG GAACAAGGTG CTGAACCACT TCAGCATCAT GCAGCAGCGG 
CGGCTCAAGG ATCAGGACCA GGACGAGGAT GAGGAGGAGA AGGAGAAACG TGGCCGCAGG 
AAGGCGAGCG AGCTGCGCAT CCACGACCTG GAGGACGACC TGGAGATGTC GTCCGATGCC 
AGTGATGCCA GTGGTGAGGA GGGGGGCAGA GTCCCCAAGG CCAAGAAGAA GGCGCCGCTG 
GCCAAGGGCG GCAGGAAAAA GAAGAAGAAG AAGGGTTCAG ACGACGAGGC CTTCGAGGAC 
AGCGATGATG GGGACTTCGA GGGCCAAGAG GTGGACTACA TGTCAGACGG CTCCAGTAGC 
TCCCAAGAAG AGCCTGAGAG CAAGGCCAAG GCGCCGCAGC AGGAGGAGGG GCCCAAGGGT 
GTCGATGAGC AGAGCGACAG TAGTGAGGAG AGTGAGGAGG AGAAGCCGCC TGAGGAGGAC 
AAGGAGGAGG AGGAGGAGAA GAAGGCACCC ACCCCGCAGG AGAAGAAGCG CAGGAAAGAC 
AGCAGCGAGG AGTCGGACAG CTCAGAGGAG AGCGACATTG ACAGCGAGGC CTCCTCAGCC 
CTCTTCATGG CGAAGAAGAA GACGCCACCC AAGAGAGAGC GGAAGCCGTC GGGAGGGAGC 
TCAAGGGGCA ACAGCCGCCC AGGCACGCCC AGCGCAGAGG GTGGCAGCAC CTCCTCCACC 
CTGCGGGCGG CTGCCAGCAA ACTCGAGCAA GGGAAGCGGG TGAGCGAGAT GCCTGCAGCC 
AAGCGGTTGC GGCTGGACAC GGGACCCCAG AGCCTGTCTG GGAAGTCGAC ACCCCAGCCA 
CCATCAGGCA AGACAACACC CAACAGCGGC GACGTGCAGG TGACTGAGGA TGCCGTGCGC 
CGCTACCTGA CACGGAAGCC CATGACCACT AAGGACCTGC TGAAAAAGTT CCAGACCAAG 
AAGACAGGGC TGAGCAGCGA GCAGACAGTG AACGTGTTGG CCCAGATCCT CAAGCGACTC 
AACCCCGAGC GCAAGATGAT CAACGACAAA ATGCACTTCT CCCTCAAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002087.2
CDS	286..1839
Translation	MAALGPSSQNVTEYVVRVPKNTTKKYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEEEMPESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTSYYIFTQCPDGAFEAFPVHNWYNFTPLARHRTLTAEEAEEEWERRNKVLNHFSIMQQRRLKDQDQDEDEEEKEKRGRRKASELRIHDLEDDLEMSSDASDASGEEGGRVPKAKKKAPLAKGGRKKKKKKGSDDEAFEDSDDGDFEGQEVDYMSDGSSSSQEEPESKAKAPQQEEGPKGVDEQSDSSEESEEEKPPEEDKEEEEEKKAPTPQEKKRRKDSSEESDSSEESDIDSEASSALFMAKKKTPPKRERKPSGGSSRGNSRPGTPSAEGGSTSSTLRAAASKLEQGKRVSEMPAAKRLRLDTGPQSLSGKSTPQPPSGKTTPNSGDVQVTEDAVRRYLTRKPMTTKDLLKKFQTKKTGLSSEQTVNVLAQILKRLNPERKMINDKMHFSLKE

Target ORF information:

RefSeq Version	NM_002096.2
Organism	Homo sapiens(human)
Definition	Homo sapiens general transcription factor IIF subunit 1 (GTF2F1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002096.2

ORF Insert Sequence:

ATGGCGGCCC TAGGCCCTAG CAGCCAGAAT GTCACTGAAT ACGTCGTTCG AGTTCCTAAG 
AATACAACCA AAAAATATAA CATCATGGCT TTTAATGCAG CCGACAAAGT CAACTTTGCT 
ACGTGGAATC AGGCTCGGCT GGAGCGGGAC TTGAGCAACA AGAAAATCTA CCAAGAGGAG 
GAGATGCCCG AATCGGGCGC GGGCAGTGAG TTCAACCGCA AGCTTCGGGA GGAGGCTCGG 
AGGAAGAAGT ACGGCATCGT CCTCAAGGAG TTCCGGCCCG AGGACCAGCC CTGGCTGCTC 
CGGGTCAACG GCAAATCAGG CAGGAAGTTC AAGGGCATCA AGAAGGGAGG CGTAACAGAG 
AACACGTCCT ACTACATCTT CACCCAGTGC CCCGACGGGG CCTTCGAGGC CTTCCCCGTG 
CACAACTGGT ACAATTTCAC ACCGCTGGCC CGGCATCGCA CGCTCACTGC CGAGGAGGCC 
GAGGAGGAGT GGGAGAGGAG GAACAAGGTG CTGAACCACT TCAGCATCAT GCAGCAGCGG 
CGGCTCAAGG ATCAGGACCA GGACGAGGAT GAGGAGGAGA AGGAGAAACG TGGCCGCAGG 
AAGGCGAGCG AGCTGCGCAT CCACGACCTG GAGGACGACC TGGAGATGTC GTCCGATGCC 
AGTGATGCCA GTGGTGAGGA GGGGGGCAGA GTCCCCAAGG CCAAGAAGAA GGCGCCGCTG 
GCCAAGGGCG GCAGGAAAAA GAAGAAGAAG AAGGGTTCAG ACGACGAGGC CTTCGAGGAC 
AGCGATGATG GGGACTTCGA GGGCCAAGAG GTGGACTACA TGTCAGACGG CTCCAGTAGC 
TCCCAAGAAG AGCCTGAGAG CAAGGCCAAG GCGCCGCAGC AGGAGGAGGG GCCCAAGGGT 
GTCGATGAGC AGAGCGACAG TAGTGAGGAG AGTGAGGAGG AGAAGCCGCC TGAGGAGGAC 
AAGGAGGAGG AGGAGGAGAA GAAGGCACCC ACCCCGCAGG AGAAGAAGCG CAGGAAAGAC 
AGCAGCGAGG AGTCGGACAG CTCAGAGGAG AGCGACATTG ACAGCGAGGC CTCCTCAGCC 
CTCTTCATGG CGAAGAAGAA GACGCCACCC AAGAGAGAGC GGAAGCCGTC GGGAGGGAGC 
TCAAGGGGCA ACAGCCGCCC AGGCACGCCC AGCGCAGAGG GTGGCAGCAC CTCCTCCACC 
CTGCGGGCGG CTGCCAGCAA ACTCGAGCAA GGGAAGCGGG TGAGCGAGAT GCCTGCAGCC 
AAGCGGTTGC GGCTGGACAC GGGACCCCAG AGCCTGTCTG GGAAGTCGAC ACCCCAGCCA 
CCATCAGGCA AGACAACACC CAACAGCGGC GACGTGCAGG TGACTGAGGA TGCCGTGCGC 
CGCTACCTGA CACGGAAGCC CATGACCACT AAGGACCTGC TGAAAAAGTT CCAGACCAAG 
AAGACAGGGC TGAGCAGCGA GCAGACAGTG AACGTGTTGG CCCAGATCCT CAAGCGACTC 
AACCCCGAGC GCAAGATGAT CAACGACAAA ATGCACTTCT CCCTCAAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTF2F1 ( XM_024451475.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307243.1 Homo sapiens general transcription factor IIF subunit 1 (GTF2F1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu104663
Clone ID Related Accession (Same CDS sequence)	XM_024451475.1
Accession Version	XM_024451475.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	general transcription factor IIF subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC TAGGCCCTAG CAGCCAGAAT GTCACTGAAT ACGTCGTTCG AGTTCCTAAG 
AATACAACCA AAAAATATAA CATCATGGCT TTTAATGCAG CCGACAAAGT CAACTTTGCT 
ACGTGGAATC AGGCTCGGCT GGAGCGGGAC TTGAGCAACA AGAAAATCTA CCAAGAGGAG 
GAGATGCCCG AATCGGGCGC GGGCAGTGAG TTCAACCGCA AGCTTCGGGA GGAGGCTCGG 
AGGAAGAAGT ACGGCATCGT CCTCAAGGAG TTCCGGCCCG AGGACCAGCC CTGGCTGCTC 
CGGGTCAACG GCAAATCAGG CAGGAAGTTC AAGGGCATCA AGAAGGGAGG CGTAACAGAG 
AACACGTCCT ACTACATCTT CACCCAGTGC CCCGACGGGG CCTTCGAGGC CTTCCCCGTG 
CACAACTGGT ACAATTTCAC ACCGCTGGCC CGGCATCGCA CGCTCACTGC CGAGGAGGCC 
GAGGAGGAGT GGGAGAGGAG GAACAAGGTG CTGAACCACT TCAGCATCAT GCAGCAGCGG 
CGGCTCAAGG ATCAGGACCA GGACGAGGAT GAGGAGGAGA AGGAGAAACG TGGCCGCAGG 
AAGGCGAGCG AGCTGCGCAT CCACGACCTG GAGGACGACC TGGAGATGTC GTCCGATGCC 
AGTGATGCCA GTGGTGAGGA GGGGGGCAGA GTCCCCAAGG CCAAGAAGAA GGCGCCGCTG 
GCCAAGGGCG GCAGGAAAAA GAAGAAGAAG AAGGGTTCAG ACGACGAGGC CTTCGAGGAC 
AGCGATGATG GGGACTTCGA GGGCCAAGAG GTGGACTACA TGTCAGACGG CTCCAGTAGC 
TCCCAAGAAG AGCCTGAGAG CAAGGCCAAG GCGCCGCAGC AGGAGGAGGG GCCCAAGGGT 
AGGCGATGGC TGGGCGGGCC GGGGAGATGG GGGCGGTGCT TCCCTGCTCT TGCTCATACC 
CTGCCCCTCC CAGGTGTCGA TGAGCAGAGC GACAGTAGTG AGGAGAGTGA GGAGGAGAAG 
CCGCCTGAGG AGGACAAGGA GGAGGAGGAG GAGAAGAAGG CACCCACCCC GCAGGAGAAG 
AAGCGCAGGA AAGACAGCAG CGAGGAGTCG GACAGCTCAG AGGAGAGCGA CATTGACAGC 
GAGGCCTCCT CAGCCCTCTT CATGGCGAAG AAGAAGACGC CACCCAAGAG AGAGCGGAAG 
CCGTCGGGAG GGAGCTCAAG GGGCAACAGC CGCCCAGGCA CGCCCAGCGC AGAGGGTGGC 
AGCACCTCCT CCACCCTGCG GGCGGCTGCC AGCAAACTCG AGCAAGGGAA GCGGGTGAGC 
GAGATGCCTG CAGCCAAGCG GTTGCGGCTG GACACGGGAC CCCAGAGCCT GTCTGGGAAG 
TCGACACCCC AGCCACCATC AGGCAAGACA ACACCCAACA GCGGCGACGT GCAGGTGACT 
GAGGATGCCG TGCGCCGCTA CCTGACACGG AAGCCCATGA CCACTAAGGA CCTGCTGAAA 
AAGTTCCAGA CCAAGAAGAC AGGGCTGAGC AGCGAGCAGA CAGTGAACGT GTTGGCCCAG 
ATCCTCAAGC GACTCAACCC CGAGCGCAAG ATGATCAACG ACAAAATGCA CTTCTCCCTC 
AAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307243.1
CDS	192..1820
Translation	MAALGPSSQNVTEYVVRVPKNTTKKYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEEEMPESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTSYYIFTQCPDGAFEAFPVHNWYNFTPLARHRTLTAEEAEEEWERRNKVLNHFSIMQQRRLKDQDQDEDEEEKEKRGRRKASELRIHDLEDDLEMSSDASDASGEEGGRVPKAKKKAPLAKGGRKKKKKKGSDDEAFEDSDDGDFEGQEVDYMSDGSSSSQEEPESKAKAPQQEEGPKGRRWLGGPGRWGRCFPALAHTLPLPGVDEQSDSSEESEEEKPPEEDKEEEEEKKAPTPQEKKRRKDSSEESDSSEESDIDSEASSALFMAKKKTPPKRERKPSGGSSRGNSRPGTPSAEGGSTSSTLRAAASKLEQGKRVSEMPAAKRLRLDTGPQSLSGKSTPQPPSGKTTPNSGDVQVTEDAVRRYLTRKPMTTKDLLKKFQTKKTGLSSEQTVNVLAQILKRLNPERKMINDKMHFSLKE

Target ORF information:

RefSeq Version	XM_024451475.1
Organism	Homo sapiens(human)
Definition	Homo sapiens general transcription factor IIF subunit 1 (GTF2F1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451475.1

ORF Insert Sequence:

ATGGCGGCCC TAGGCCCTAG CAGCCAGAAT GTCACTGAAT ACGTCGTTCG AGTTCCTAAG 
AATACAACCA AAAAATATAA CATCATGGCT TTTAATGCAG CCGACAAAGT CAACTTTGCT 
ACGTGGAATC AGGCTCGGCT GGAGCGGGAC TTGAGCAACA AGAAAATCTA CCAAGAGGAG 
GAGATGCCCG AATCGGGCGC GGGCAGTGAG TTCAACCGCA AGCTTCGGGA GGAGGCTCGG 
AGGAAGAAGT ACGGCATCGT CCTCAAGGAG TTCCGGCCCG AGGACCAGCC CTGGCTGCTC 
CGGGTCAACG GCAAATCAGG CAGGAAGTTC AAGGGCATCA AGAAGGGAGG CGTAACAGAG 
AACACGTCCT ACTACATCTT CACCCAGTGC CCCGACGGGG CCTTCGAGGC CTTCCCCGTG 
CACAACTGGT ACAATTTCAC ACCGCTGGCC CGGCATCGCA CGCTCACTGC CGAGGAGGCC 
GAGGAGGAGT GGGAGAGGAG GAACAAGGTG CTGAACCACT TCAGCATCAT GCAGCAGCGG 
CGGCTCAAGG ATCAGGACCA GGACGAGGAT GAGGAGGAGA AGGAGAAACG TGGCCGCAGG 
AAGGCGAGCG AGCTGCGCAT CCACGACCTG GAGGACGACC TGGAGATGTC GTCCGATGCC 
AGTGATGCCA GTGGTGAGGA GGGGGGCAGA GTCCCCAAGG CCAAGAAGAA GGCGCCGCTG 
GCCAAGGGCG GCAGGAAAAA GAAGAAGAAG AAGGGTTCAG ACGACGAGGC CTTCGAGGAC 
AGCGATGATG GGGACTTCGA GGGCCAAGAG GTGGACTACA TGTCAGACGG CTCCAGTAGC 
TCCCAAGAAG AGCCTGAGAG CAAGGCCAAG GCGCCGCAGC AGGAGGAGGG GCCCAAGGGT 
AGGCGATGGC TGGGCGGGCC GGGGAGATGG GGGCGGTGCT TCCCTGCTCT TGCTCATACC 
CTGCCCCTCC CAGGTGTCGA TGAGCAGAGC GACAGTAGTG AGGAGAGTGA GGAGGAGAAG 
CCGCCTGAGG AGGACAAGGA GGAGGAGGAG GAGAAGAAGG CACCCACCCC GCAGGAGAAG 
AAGCGCAGGA AAGACAGCAG CGAGGAGTCG GACAGCTCAG AGGAGAGCGA CATTGACAGC 
GAGGCCTCCT CAGCCCTCTT CATGGCGAAG AAGAAGACGC CACCCAAGAG AGAGCGGAAG 
CCGTCGGGAG GGAGCTCAAG GGGCAACAGC CGCCCAGGCA CGCCCAGCGC AGAGGGTGGC 
AGCACCTCCT CCACCCTGCG GGCGGCTGCC AGCAAACTCG AGCAAGGGAA GCGGGTGAGC 
GAGATGCCTG CAGCCAAGCG GTTGCGGCTG GACACGGGAC CCCAGAGCCT GTCTGGGAAG 
TCGACACCCC AGCCACCATC AGGCAAGACA ACACCCAACA GCGGCGACGT GCAGGTGACT 
GAGGATGCCG TGCGCCGCTA CCTGACACGG AAGCCCATGA CCACTAAGGA CCTGCTGAAA 
AAGTTCCAGA CCAAGAAGAC AGGGCTGAGC AGCGAGCAGA CAGTGAACGT GTTGGCCCAG 
ATCCTCAAGC GACTCAACCC CGAGCGCAAG ATGATCAACG ACAAAATGCA CTTCTCCCTC 
AAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of Androgen Receptor Activity by Transient Interactions of Its Transactivation Domain with General Transcription Regulators.
Structure (London, England : 1993)26(1)145-152.e3(2018 Jan)
De Mol E,Szulc E,Di Sanza C,Mart?nez-Crist?bal P,Bertoncini CW,Fenwick RB,Frigol?-Vivas M,Mas?n M,Hunter I,Buz?n V,Brun-Heath I,Garc?a J,De Fabritiis G,Est?banez-Perpi?? E,McEwan IJ,Nebreda ?R,Salvatella X

Near-atomic resolution visualization of human transcription promoter opening.
Nature533(7603)359-65(2016 05)
He Y,Yan C,Fang J,Inouye C,Tjian R,Ivanov I,Nogales E

A new paradigm for transcription factor TFIIB functionality.
Scientific reports43664(2014 Jan)
Gelev V,Zabolotny JM,Lange M,Hiromura M,Yoo SW,Orlando JS,Kushnir A,Horikoshi N,Paquet E,Bachvarov D,Schaffer PA,Usheva A

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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GTF2F1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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