GRM5 cDNA ORF clone, Homo sapiens(human)

The following GRM5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRM5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu28015 NM_000842.4
Latest version!
Homo sapiens glutamate metabotropic receptor 5 (GRM5), transcript variant b, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu28022 NM_001143831.2
Latest version!
Homo sapiens glutamate metabotropic receptor 5 (GRM5), transcript variant a, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu28015 XM_017017627.2
Latest version!
Homo sapiens glutamate metabotropic receptor 5 (GRM5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu28022 XM_011542793.1
Latest version!
Homo sapiens glutamate receptor, metabotropic 5 (GRM5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu28022 XM_011542794.1
Latest version!
Homo sapiens glutamate receptor, metabotropic 5 (GRM5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu28022 XM_006718828.4
Latest version!
Homo sapiens glutamate metabotropic receptor 5 (GRM5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu28022 XM_011542792.1
Latest version!
Homo sapiens glutamate metabotropic receptor 5 (GRM5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
View Old Accession Versions >>
OHu28022 XM_006718828.2 Homo sapiens glutamate receptor, metabotropic 5 (GRM5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu28015 XM_017017627.1 Homo sapiens glutamate metabotropic receptor 5 (GRM5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu28022 XM_006718828.3 Homo sapiens glutamate metabotropic receptor 5 (GRM5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu28015
    Clone ID Related Accession (Same CDS sequence) XM_017017627.2 , NM_000842.4 , XM_017017627.1
    Accession Version NM_000842.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product metabotropic glutamate receptor 5 isoform b precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA316590.1, AK291362.1 and AP003120.4. This sequence is a reference standard in the RefSeqGene project. On May 14, 2014 this sequence version replaced NM_000842.3. Transcript Variant: This variant (b) has a longer 5' UTR and lacks an in-frame exon in the 3' coding region, compared to variant a. The encoded isoform (b, also known as mGluR5a) is shorter, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291362.1, SRR1803616.255620.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG TTCCAATGGA
    AAATCCGTCA CGTGGGCCCA GAATGAGAAG AGCAGCCGGG GGCAGCACCT GTGGCAGCGC
    CTGTCCATCC ACATCAACAA GAAAGAAAAC CCCAACCAAA CGGCCGTCAT CAAGCCCTTC
    CCCAAGAGCA CGGAGAGCCG TGGCCTGGGC GCTGGCGCTG GCGCAGGCGG GAGCGCTGGG
    GGCGTGGGGG CCACGGGCGG TGCGGGCTGC GCAGGCGCCG GCCCAGGCGG GCCCGAGTCC
    CCAGACGCCG GCCCCAAGGC GCTGTATGAT GTGGCCGAGG CTGAGGAGCA CTTCCCGGCG
    CCCGCGCGGC CGCGCTCACC GTCGCCCATC AGCACGCTGA GCCACCGCGC GGGCTCGGCC
    AGCCGCACGG ACGACGATGT GCCGTCGCTG CACTCGGAGC CTGTGGCGCG CAGCAGCTCC
    TCGCAGGGCT CCCTCATGGA GCAGATCAGC AGTGTGGTCA CCCGCTTCAC GGCCAACATC
    AGCGAGCTCA ACTCCATGAT GCTGTCCACC GCGGCCCCCA GCCCCGGCGT CGGCGCCCCG
    CTCTGCTCGT CCTACCTGAT CCCCAAAGAG ATCCAGTTGC CCACGACCAT GACGACCTTT
    GCCGAAATCC AGCCTCTGCC GGCCATCGAA GTCACGGGAG GCGCGCAGCC CGCGGCAGGG
    GCGCAGGCGG CTGGGGACGC GGCCCGGGAG AGCCCCGCGG CCGGTCCCGA GGCTGCGGCC
    GCCAAGCCAG ACCTGGAGGA GCTGGTGGCT CTCACCCCGC CGTCCCCCTT CAGAGACTCG
    GTGGACTCGG GGAGCACAAC CCCCAACTCG CCAGTGTCCG AGTCGGCCCT CTGTATCCCG
    TCGTCTCCCA AATATGACAC TCTTATCATA AGAGATTACA CTCAGAGCTC CTCGTCGTTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000833.1
    CDS399..3941
    Translation

    Target ORF information:

    RefSeq Version NM_000842.4
    Organism Homo sapiens(human)
    Definition Homo sapiens glutamate metabotropic receptor 5 (GRM5), transcript variant b, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000842.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG TTCCAATGGA
    AAATCCGTCA CGTGGGCCCA GAATGAGAAG AGCAGCCGGG GGCAGCACCT GTGGCAGCGC
    CTGTCCATCC ACATCAACAA GAAAGAAAAC CCCAACCAAA CGGCCGTCAT CAAGCCCTTC
    CCCAAGAGCA CGGAGAGCCG TGGCCTGGGC GCTGGCGCTG GCGCAGGCGG GAGCGCTGGG
    GGCGTGGGGG CCACGGGCGG TGCGGGCTGC GCAGGCGCCG GCCCAGGCGG GCCCGAGTCC
    CCAGACGCCG GCCCCAAGGC GCTGTATGAT GTGGCCGAGG CTGAGGAGCA CTTCCCGGCG
    CCCGCGCGGC CGCGCTCACC GTCGCCCATC AGCACGCTGA GCCACCGCGC GGGCTCGGCC
    AGCCGCACGG ACGACGATGT GCCGTCGCTG CACTCGGAGC CTGTGGCGCG CAGCAGCTCC
    TCGCAGGGCT CCCTCATGGA GCAGATCAGC AGTGTGGTCA CCCGCTTCAC GGCCAACATC
    AGCGAGCTCA ACTCCATGAT GCTGTCCACC GCGGCCCCCA GCCCCGGCGT CGGCGCCCCG
    CTCTGCTCGT CCTACCTGAT CCCCAAAGAG ATCCAGTTGC CCACGACCAT GACGACCTTT
    GCCGAAATCC AGCCTCTGCC GGCCATCGAA GTCACGGGAG GCGCGCAGCC CGCGGCAGGG
    GCGCAGGCGG CTGGGGACGC GGCCCGGGAG AGCCCCGCGG CCGGTCCCGA GGCTGCGGCC
    GCCAAGCCAG ACCTGGAGGA GCTGGTGGCT CTCACCCCGC CGTCCCCCTT CAGAGACTCG
    GTGGACTCGG GGAGCACAAC CCCCAACTCG CCAGTGTCCG AGTCGGCCCT CTGTATCCCG
    TCGTCTCCCA AATATGACAC TCTTATCATA AGAGATTACA CTCAGAGCTC CTCGTCGTTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28022
    Clone ID Related Accession (Same CDS sequence) XM_011542793.1 , XM_011542794.1 , XM_011542792.1 , NM_001143831.2 , XM_006718828.3 , XM_006718828.2 , XM_006718828.4
    Accession Version NM_001143831.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-19
    Organism Homo sapiens(human)
    Product metabotropic glutamate receptor 5 isoform a precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC031602.1, D28539.1 and AP003120.4. On Mar 31, 2009 this sequence version replaced NM_001143831.1. Transcript Variant: This variant (a) encodes the longer isoform (a, also known as mGluR5b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D28539.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG GTACAAAGAC
    AGGAGACTGG CCCAGCACAA GTCGGAAATA GAGTGTTTCA CCCCCAAAGG GAGTATGGGG
    AATGGTGGGA GAGCAACAAT GAGCAGTTCC AATGGAAAAT CCGTCACGTG GGCCCAGAAT
    GAGAAGAGCA GCCGGGGGCA GCACCTGTGG CAGCGCCTGT CCATCCACAT CAACAAGAAA
    GAAAACCCCA ACCAAACGGC CGTCATCAAG CCCTTCCCCA AGAGCACGGA GAGCCGTGGC
    CTGGGCGCTG GCGCTGGCGC AGGCGGGAGC GCTGGGGGCG TGGGGGCCAC GGGCGGTGCG
    GGCTGCGCAG GCGCCGGCCC AGGCGGGCCC GAGTCCCCAG ACGCCGGCCC CAAGGCGCTG
    TATGATGTGG CCGAGGCTGA GGAGCACTTC CCGGCGCCCG CGCGGCCGCG CTCACCGTCG
    CCCATCAGCA CGCTGAGCCA CCGCGCGGGC TCGGCCAGCC GCACGGACGA CGATGTGCCG
    TCGCTGCACT CGGAGCCTGT GGCGCGCAGC AGCTCCTCGC AGGGCTCCCT CATGGAGCAG
    ATCAGCAGTG TGGTCACCCG CTTCACGGCC AACATCAGCG AGCTCAACTC CATGATGCTG
    TCCACCGCGG CCCCCAGCCC CGGCGTCGGC GCCCCGCTCT GCTCGTCCTA CCTGATCCCC
    AAAGAGATCC AGTTGCCCAC GACCATGACG ACCTTTGCCG AAATCCAGCC TCTGCCGGCC
    ATCGAAGTCA CGGGAGGCGC GCAGCCCGCG GCAGGGGCGC AGGCGGCTGG GGACGCGGCC
    CGGGAGAGCC CCGCGGCCGG TCCCGAGGCT GCGGCCGCCA AGCCAGACCT GGAGGAGCTG
    GTGGCTCTCA CCCCGCCGTC CCCCTTCAGA GACTCGGTGG ACTCGGGGAG CACAACCCCC
    AACTCGCCAG TGTCCGAGTC GGCCCTCTGT ATCCCGTCGT CTCCCAAATA TGACACTCTT
    ATCATAAGAG ATTACACTCA GAGCTCCTCG TCGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137303.1
    CDS201..3839
    Translation

    Target ORF information:

    RefSeq Version NM_001143831.2
    Organism Homo sapiens(human)
    Definition Homo sapiens glutamate metabotropic receptor 5 (GRM5), transcript variant a, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001143831.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG GTACAAAGAC
    AGGAGACTGG CCCAGCACAA GTCGGAAATA GAGTGTTTCA CCCCCAAAGG GAGTATGGGG
    AATGGTGGGA GAGCAACAAT GAGCAGTTCC AATGGAAAAT CCGTCACGTG GGCCCAGAAT
    GAGAAGAGCA GCCGGGGGCA GCACCTGTGG CAGCGCCTGT CCATCCACAT CAACAAGAAA
    GAAAACCCCA ACCAAACGGC CGTCATCAAG CCCTTCCCCA AGAGCACGGA GAGCCGTGGC
    CTGGGCGCTG GCGCTGGCGC AGGCGGGAGC GCTGGGGGCG TGGGGGCCAC GGGCGGTGCG
    GGCTGCGCAG GCGCCGGCCC AGGCGGGCCC GAGTCCCCAG ACGCCGGCCC CAAGGCGCTG
    TATGATGTGG CCGAGGCTGA GGAGCACTTC CCGGCGCCCG CGCGGCCGCG CTCACCGTCG
    CCCATCAGCA CGCTGAGCCA CCGCGCGGGC TCGGCCAGCC GCACGGACGA CGATGTGCCG
    TCGCTGCACT CGGAGCCTGT GGCGCGCAGC AGCTCCTCGC AGGGCTCCCT CATGGAGCAG
    ATCAGCAGTG TGGTCACCCG CTTCACGGCC AACATCAGCG AGCTCAACTC CATGATGCTG
    TCCACCGCGG CCCCCAGCCC CGGCGTCGGC GCCCCGCTCT GCTCGTCCTA CCTGATCCCC
    AAAGAGATCC AGTTGCCCAC GACCATGACG ACCTTTGCCG AAATCCAGCC TCTGCCGGCC
    ATCGAAGTCA CGGGAGGCGC GCAGCCCGCG GCAGGGGCGC AGGCGGCTGG GGACGCGGCC
    CGGGAGAGCC CCGCGGCCGG TCCCGAGGCT GCGGCCGCCA AGCCAGACCT GGAGGAGCTG
    GTGGCTCTCA CCCCGCCGTC CCCCTTCAGA GACTCGGTGG ACTCGGGGAG CACAACCCCC
    AACTCGCCAG TGTCCGAGTC GGCCCTCTGT ATCCCGTCGT CTCCCAAATA TGACACTCTT
    ATCATAAGAG ATTACACTCA GAGCTCCTCG TCGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28015
    Clone ID Related Accession (Same CDS sequence) XM_017017627.2 , NM_000842.4 , XM_017017627.1
    Accession Version XM_017017627.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product metabotropic glutamate receptor 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017017627.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG TTCCAATGGA
    AAATCCGTCA CGTGGGCCCA GAATGAGAAG AGCAGCCGGG GGCAGCACCT GTGGCAGCGC
    CTGTCCATCC ACATCAACAA GAAAGAAAAC CCCAACCAAA CGGCCGTCAT CAAGCCCTTC
    CCCAAGAGCA CGGAGAGCCG TGGCCTGGGC GCTGGCGCTG GCGCAGGCGG GAGCGCTGGG
    GGCGTGGGGG CCACGGGCGG TGCGGGCTGC GCAGGCGCCG GCCCAGGCGG GCCCGAGTCC
    CCAGACGCCG GCCCCAAGGC GCTGTATGAT GTGGCCGAGG CTGAGGAGCA CTTCCCGGCG
    CCCGCGCGGC CGCGCTCACC GTCGCCCATC AGCACGCTGA GCCACCGCGC GGGCTCGGCC
    AGCCGCACGG ACGACGATGT GCCGTCGCTG CACTCGGAGC CTGTGGCGCG CAGCAGCTCC
    TCGCAGGGCT CCCTCATGGA GCAGATCAGC AGTGTGGTCA CCCGCTTCAC GGCCAACATC
    AGCGAGCTCA ACTCCATGAT GCTGTCCACC GCGGCCCCCA GCCCCGGCGT CGGCGCCCCG
    CTCTGCTCGT CCTACCTGAT CCCCAAAGAG ATCCAGTTGC CCACGACCAT GACGACCTTT
    GCCGAAATCC AGCCTCTGCC GGCCATCGAA GTCACGGGAG GCGCGCAGCC CGCGGCAGGG
    GCGCAGGCGG CTGGGGACGC GGCCCGGGAG AGCCCCGCGG CCGGTCCCGA GGCTGCGGCC
    GCCAAGCCAG ACCTGGAGGA GCTGGTGGCT CTCACCCCGC CGTCCCCCTT CAGAGACTCG
    GTGGACTCGG GGAGCACAAC CCCCAACTCG CCAGTGTCCG AGTCGGCCCT CTGTATCCCG
    TCGTCTCCCA AATATGACAC TCTTATCATA AGAGATTACA CTCAGAGCTC CTCGTCGTTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873116.1
    CDS389..3931
    Translation

    Target ORF information:

    RefSeq Version XM_017017627.2
    Organism Homo sapiens(human)
    Definition Homo sapiens glutamate metabotropic receptor 5 (GRM5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017017627.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG TTCCAATGGA
    AAATCCGTCA CGTGGGCCCA GAATGAGAAG AGCAGCCGGG GGCAGCACCT GTGGCAGCGC
    CTGTCCATCC ACATCAACAA GAAAGAAAAC CCCAACCAAA CGGCCGTCAT CAAGCCCTTC
    CCCAAGAGCA CGGAGAGCCG TGGCCTGGGC GCTGGCGCTG GCGCAGGCGG GAGCGCTGGG
    GGCGTGGGGG CCACGGGCGG TGCGGGCTGC GCAGGCGCCG GCCCAGGCGG GCCCGAGTCC
    CCAGACGCCG GCCCCAAGGC GCTGTATGAT GTGGCCGAGG CTGAGGAGCA CTTCCCGGCG
    CCCGCGCGGC CGCGCTCACC GTCGCCCATC AGCACGCTGA GCCACCGCGC GGGCTCGGCC
    AGCCGCACGG ACGACGATGT GCCGTCGCTG CACTCGGAGC CTGTGGCGCG CAGCAGCTCC
    TCGCAGGGCT CCCTCATGGA GCAGATCAGC AGTGTGGTCA CCCGCTTCAC GGCCAACATC
    AGCGAGCTCA ACTCCATGAT GCTGTCCACC GCGGCCCCCA GCCCCGGCGT CGGCGCCCCG
    CTCTGCTCGT CCTACCTGAT CCCCAAAGAG ATCCAGTTGC CCACGACCAT GACGACCTTT
    GCCGAAATCC AGCCTCTGCC GGCCATCGAA GTCACGGGAG GCGCGCAGCC CGCGGCAGGG
    GCGCAGGCGG CTGGGGACGC GGCCCGGGAG AGCCCCGCGG CCGGTCCCGA GGCTGCGGCC
    GCCAAGCCAG ACCTGGAGGA GCTGGTGGCT CTCACCCCGC CGTCCCCCTT CAGAGACTCG
    GTGGACTCGG GGAGCACAAC CCCCAACTCG CCAGTGTCCG AGTCGGCCCT CTGTATCCCG
    TCGTCTCCCA AATATGACAC TCTTATCATA AGAGATTACA CTCAGAGCTC CTCGTCGTTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28015
    Clone ID Related Accession (Same CDS sequence) XM_017017627.2 , NM_000842.4 , XM_017017627.1
    Accession Version XM_017017627.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product metabotropic glutamate receptor 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG TTCCAATGGA
    AAATCCGTCA CGTGGGCCCA GAATGAGAAG AGCAGCCGGG GGCAGCACCT GTGGCAGCGC
    CTGTCCATCC ACATCAACAA GAAAGAAAAC CCCAACCAAA CGGCCGTCAT CAAGCCCTTC
    CCCAAGAGCA CGGAGAGCCG TGGCCTGGGC GCTGGCGCTG GCGCAGGCGG GAGCGCTGGG
    GGCGTGGGGG CCACGGGCGG TGCGGGCTGC GCAGGCGCCG GCCCAGGCGG GCCCGAGTCC
    CCAGACGCCG GCCCCAAGGC GCTGTATGAT GTGGCCGAGG CTGAGGAGCA CTTCCCGGCG
    CCCGCGCGGC CGCGCTCACC GTCGCCCATC AGCACGCTGA GCCACCGCGC GGGCTCGGCC
    AGCCGCACGG ACGACGATGT GCCGTCGCTG CACTCGGAGC CTGTGGCGCG CAGCAGCTCC
    TCGCAGGGCT CCCTCATGGA GCAGATCAGC AGTGTGGTCA CCCGCTTCAC GGCCAACATC
    AGCGAGCTCA ACTCCATGAT GCTGTCCACC GCGGCCCCCA GCCCCGGCGT CGGCGCCCCG
    CTCTGCTCGT CCTACCTGAT CCCCAAAGAG ATCCAGTTGC CCACGACCAT GACGACCTTT
    GCCGAAATCC AGCCTCTGCC GGCCATCGAA GTCACGGGAG GCGCGCAGCC CGCGGCAGGG
    GCGCAGGCGG CTGGGGACGC GGCCCGGGAG AGCCCCGCGG CCGGTCCCGA GGCTGCGGCC
    GCCAAGCCAG ACCTGGAGGA GCTGGTGGCT CTCACCCCGC CGTCCCCCTT CAGAGACTCG
    GTGGACTCGG GGAGCACAAC CCCCAACTCG CCAGTGTCCG AGTCGGCCCT CTGTATCCCG
    TCGTCTCCCA AATATGACAC TCTTATCATA AGAGATTACA CTCAGAGCTC CTCGTCGTTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873116.1
    CDS397..3939
    Translation

    Target ORF information:

    RefSeq Version XM_017017627.1
    Organism Homo sapiens(human)
    Definition Homo sapiens glutamate metabotropic receptor 5 (GRM5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017017627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG TTCCAATGGA
    AAATCCGTCA CGTGGGCCCA GAATGAGAAG AGCAGCCGGG GGCAGCACCT GTGGCAGCGC
    CTGTCCATCC ACATCAACAA GAAAGAAAAC CCCAACCAAA CGGCCGTCAT CAAGCCCTTC
    CCCAAGAGCA CGGAGAGCCG TGGCCTGGGC GCTGGCGCTG GCGCAGGCGG GAGCGCTGGG
    GGCGTGGGGG CCACGGGCGG TGCGGGCTGC GCAGGCGCCG GCCCAGGCGG GCCCGAGTCC
    CCAGACGCCG GCCCCAAGGC GCTGTATGAT GTGGCCGAGG CTGAGGAGCA CTTCCCGGCG
    CCCGCGCGGC CGCGCTCACC GTCGCCCATC AGCACGCTGA GCCACCGCGC GGGCTCGGCC
    AGCCGCACGG ACGACGATGT GCCGTCGCTG CACTCGGAGC CTGTGGCGCG CAGCAGCTCC
    TCGCAGGGCT CCCTCATGGA GCAGATCAGC AGTGTGGTCA CCCGCTTCAC GGCCAACATC
    AGCGAGCTCA ACTCCATGAT GCTGTCCACC GCGGCCCCCA GCCCCGGCGT CGGCGCCCCG
    CTCTGCTCGT CCTACCTGAT CCCCAAAGAG ATCCAGTTGC CCACGACCAT GACGACCTTT
    GCCGAAATCC AGCCTCTGCC GGCCATCGAA GTCACGGGAG GCGCGCAGCC CGCGGCAGGG
    GCGCAGGCGG CTGGGGACGC GGCCCGGGAG AGCCCCGCGG CCGGTCCCGA GGCTGCGGCC
    GCCAAGCCAG ACCTGGAGGA GCTGGTGGCT CTCACCCCGC CGTCCCCCTT CAGAGACTCG
    GTGGACTCGG GGAGCACAAC CCCCAACTCG CCAGTGTCCG AGTCGGCCCT CTGTATCCCG
    TCGTCTCCCA AATATGACAC TCTTATCATA AGAGATTACA CTCAGAGCTC CTCGTCGTTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28022
    Clone ID Related Accession (Same CDS sequence) XM_011542793.1 , XM_011542794.1 , XM_011542792.1 , NM_001143831.2 , XM_006718828.3 , XM_006718828.2 , XM_006718828.4
    Accession Version XM_011542793.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product metabotropic glutamate receptor 5 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG GTACAAAGAC
    AGGAGACTGG CCCAGCACAA GTCGGAAATA GAGTGTTTCA CCCCCAAAGG GAGTATGGGG
    AATGGTGGGA GAGCAACAAT GAGCAGTTCC AATGGAAAAT CCGTCACGTG GGCCCAGAAT
    GAGAAGAGCA GCCGGGGGCA GCACCTGTGG CAGCGCCTGT CCATCCACAT CAACAAGAAA
    GAAAACCCCA ACCAAACGGC CGTCATCAAG CCCTTCCCCA AGAGCACGGA GAGCCGTGGC
    CTGGGCGCTG GCGCTGGCGC AGGCGGGAGC GCTGGGGGCG TGGGGGCCAC GGGCGGTGCG
    GGCTGCGCAG GCGCCGGCCC AGGCGGGCCC GAGTCCCCAG ACGCCGGCCC CAAGGCGCTG
    TATGATGTGG CCGAGGCTGA GGAGCACTTC CCGGCGCCCG CGCGGCCGCG CTCACCGTCG
    CCCATCAGCA CGCTGAGCCA CCGCGCGGGC TCGGCCAGCC GCACGGACGA CGATGTGCCG
    TCGCTGCACT CGGAGCCTGT GGCGCGCAGC AGCTCCTCGC AGGGCTCCCT CATGGAGCAG
    ATCAGCAGTG TGGTCACCCG CTTCACGGCC AACATCAGCG AGCTCAACTC CATGATGCTG
    TCCACCGCGG CCCCCAGCCC CGGCGTCGGC GCCCCGCTCT GCTCGTCCTA CCTGATCCCC
    AAAGAGATCC AGTTGCCCAC GACCATGACG ACCTTTGCCG AAATCCAGCC TCTGCCGGCC
    ATCGAAGTCA CGGGAGGCGC GCAGCCCGCG GCAGGGGCGC AGGCGGCTGG GGACGCGGCC
    CGGGAGAGCC CCGCGGCCGG TCCCGAGGCT GCGGCCGCCA AGCCAGACCT GGAGGAGCTG
    GTGGCTCTCA CCCCGCCGTC CCCCTTCAGA GACTCGGTGG ACTCGGGGAG CACAACCCCC
    AACTCGCCAG TGTCCGAGTC GGCCCTCTGT ATCCCGTCGT CTCCCAAATA TGACACTCTT
    ATCATAAGAG ATTACACTCA GAGCTCCTCG TCGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011541095.1
    Translation

    Target ORF information:

    RefSeq Version XM_011542793.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens glutamate receptor, metabotropic 5 (GRM5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011542793.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG GTACAAAGAC
    AGGAGACTGG CCCAGCACAA GTCGGAAATA GAGTGTTTCA CCCCCAAAGG GAGTATGGGG
    AATGGTGGGA GAGCAACAAT GAGCAGTTCC AATGGAAAAT CCGTCACGTG GGCCCAGAAT
    GAGAAGAGCA GCCGGGGGCA GCACCTGTGG CAGCGCCTGT CCATCCACAT CAACAAGAAA
    GAAAACCCCA ACCAAACGGC CGTCATCAAG CCCTTCCCCA AGAGCACGGA GAGCCGTGGC
    CTGGGCGCTG GCGCTGGCGC AGGCGGGAGC GCTGGGGGCG TGGGGGCCAC GGGCGGTGCG
    GGCTGCGCAG GCGCCGGCCC AGGCGGGCCC GAGTCCCCAG ACGCCGGCCC CAAGGCGCTG
    TATGATGTGG CCGAGGCTGA GGAGCACTTC CCGGCGCCCG CGCGGCCGCG CTCACCGTCG
    CCCATCAGCA CGCTGAGCCA CCGCGCGGGC TCGGCCAGCC GCACGGACGA CGATGTGCCG
    TCGCTGCACT CGGAGCCTGT GGCGCGCAGC AGCTCCTCGC AGGGCTCCCT CATGGAGCAG
    ATCAGCAGTG TGGTCACCCG CTTCACGGCC AACATCAGCG AGCTCAACTC CATGATGCTG
    TCCACCGCGG CCCCCAGCCC CGGCGTCGGC GCCCCGCTCT GCTCGTCCTA CCTGATCCCC
    AAAGAGATCC AGTTGCCCAC GACCATGACG ACCTTTGCCG AAATCCAGCC TCTGCCGGCC
    ATCGAAGTCA CGGGAGGCGC GCAGCCCGCG GCAGGGGCGC AGGCGGCTGG GGACGCGGCC
    CGGGAGAGCC CCGCGGCCGG TCCCGAGGCT GCGGCCGCCA AGCCAGACCT GGAGGAGCTG
    GTGGCTCTCA CCCCGCCGTC CCCCTTCAGA GACTCGGTGG ACTCGGGGAG CACAACCCCC
    AACTCGCCAG TGTCCGAGTC GGCCCTCTGT ATCCCGTCGT CTCCCAAATA TGACACTCTT
    ATCATAAGAG ATTACACTCA GAGCTCCTCG TCGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28022
    Clone ID Related Accession (Same CDS sequence) XM_011542793.1 , XM_011542794.1 , XM_011542792.1 , NM_001143831.2 , XM_006718828.3 , XM_006718828.2 , XM_006718828.4
    Accession Version XM_011542794.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product metabotropic glutamate receptor 5 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG GTACAAAGAC
    AGGAGACTGG CCCAGCACAA GTCGGAAATA GAGTGTTTCA CCCCCAAAGG GAGTATGGGG
    AATGGTGGGA GAGCAACAAT GAGCAGTTCC AATGGAAAAT CCGTCACGTG GGCCCAGAAT
    GAGAAGAGCA GCCGGGGGCA GCACCTGTGG CAGCGCCTGT CCATCCACAT CAACAAGAAA
    GAAAACCCCA ACCAAACGGC CGTCATCAAG CCCTTCCCCA AGAGCACGGA GAGCCGTGGC
    CTGGGCGCTG GCGCTGGCGC AGGCGGGAGC GCTGGGGGCG TGGGGGCCAC GGGCGGTGCG
    GGCTGCGCAG GCGCCGGCCC AGGCGGGCCC GAGTCCCCAG ACGCCGGCCC CAAGGCGCTG
    TATGATGTGG CCGAGGCTGA GGAGCACTTC CCGGCGCCCG CGCGGCCGCG CTCACCGTCG
    CCCATCAGCA CGCTGAGCCA CCGCGCGGGC TCGGCCAGCC GCACGGACGA CGATGTGCCG
    TCGCTGCACT CGGAGCCTGT GGCGCGCAGC AGCTCCTCGC AGGGCTCCCT CATGGAGCAG
    ATCAGCAGTG TGGTCACCCG CTTCACGGCC AACATCAGCG AGCTCAACTC CATGATGCTG
    TCCACCGCGG CCCCCAGCCC CGGCGTCGGC GCCCCGCTCT GCTCGTCCTA CCTGATCCCC
    AAAGAGATCC AGTTGCCCAC GACCATGACG ACCTTTGCCG AAATCCAGCC TCTGCCGGCC
    ATCGAAGTCA CGGGAGGCGC GCAGCCCGCG GCAGGGGCGC AGGCGGCTGG GGACGCGGCC
    CGGGAGAGCC CCGCGGCCGG TCCCGAGGCT GCGGCCGCCA AGCCAGACCT GGAGGAGCTG
    GTGGCTCTCA CCCCGCCGTC CCCCTTCAGA GACTCGGTGG ACTCGGGGAG CACAACCCCC
    AACTCGCCAG TGTCCGAGTC GGCCCTCTGT ATCCCGTCGT CTCCCAAATA TGACACTCTT
    ATCATAAGAG ATTACACTCA GAGCTCCTCG TCGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011541096.1
    Translation

    Target ORF information:

    RefSeq Version XM_011542794.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens glutamate receptor, metabotropic 5 (GRM5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011542794.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG GTACAAAGAC
    AGGAGACTGG CCCAGCACAA GTCGGAAATA GAGTGTTTCA CCCCCAAAGG GAGTATGGGG
    AATGGTGGGA GAGCAACAAT GAGCAGTTCC AATGGAAAAT CCGTCACGTG GGCCCAGAAT
    GAGAAGAGCA GCCGGGGGCA GCACCTGTGG CAGCGCCTGT CCATCCACAT CAACAAGAAA
    GAAAACCCCA ACCAAACGGC CGTCATCAAG CCCTTCCCCA AGAGCACGGA GAGCCGTGGC
    CTGGGCGCTG GCGCTGGCGC AGGCGGGAGC GCTGGGGGCG TGGGGGCCAC GGGCGGTGCG
    GGCTGCGCAG GCGCCGGCCC AGGCGGGCCC GAGTCCCCAG ACGCCGGCCC CAAGGCGCTG
    TATGATGTGG CCGAGGCTGA GGAGCACTTC CCGGCGCCCG CGCGGCCGCG CTCACCGTCG
    CCCATCAGCA CGCTGAGCCA CCGCGCGGGC TCGGCCAGCC GCACGGACGA CGATGTGCCG
    TCGCTGCACT CGGAGCCTGT GGCGCGCAGC AGCTCCTCGC AGGGCTCCCT CATGGAGCAG
    ATCAGCAGTG TGGTCACCCG CTTCACGGCC AACATCAGCG AGCTCAACTC CATGATGCTG
    TCCACCGCGG CCCCCAGCCC CGGCGTCGGC GCCCCGCTCT GCTCGTCCTA CCTGATCCCC
    AAAGAGATCC AGTTGCCCAC GACCATGACG ACCTTTGCCG AAATCCAGCC TCTGCCGGCC
    ATCGAAGTCA CGGGAGGCGC GCAGCCCGCG GCAGGGGCGC AGGCGGCTGG GGACGCGGCC
    CGGGAGAGCC CCGCGGCCGG TCCCGAGGCT GCGGCCGCCA AGCCAGACCT GGAGGAGCTG
    GTGGCTCTCA CCCCGCCGTC CCCCTTCAGA GACTCGGTGG ACTCGGGGAG CACAACCCCC
    AACTCGCCAG TGTCCGAGTC GGCCCTCTGT ATCCCGTCGT CTCCCAAATA TGACACTCTT
    ATCATAAGAG ATTACACTCA GAGCTCCTCG TCGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28022
    Clone ID Related Accession (Same CDS sequence) XM_011542793.1 , XM_011542794.1 , XM_011542792.1 , NM_001143831.2 , XM_006718828.3 , XM_006718828.2 , XM_006718828.4
    Accession Version XM_011542792.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product metabotropic glutamate receptor 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG GTACAAAGAC
    AGGAGACTGG CCCAGCACAA GTCGGAAATA GAGTGTTTCA CCCCCAAAGG GAGTATGGGG
    AATGGTGGGA GAGCAACAAT GAGCAGTTCC AATGGAAAAT CCGTCACGTG GGCCCAGAAT
    GAGAAGAGCA GCCGGGGGCA GCACCTGTGG CAGCGCCTGT CCATCCACAT CAACAAGAAA
    GAAAACCCCA ACCAAACGGC CGTCATCAAG CCCTTCCCCA AGAGCACGGA GAGCCGTGGC
    CTGGGCGCTG GCGCTGGCGC AGGCGGGAGC GCTGGGGGCG TGGGGGCCAC GGGCGGTGCG
    GGCTGCGCAG GCGCCGGCCC AGGCGGGCCC GAGTCCCCAG ACGCCGGCCC CAAGGCGCTG
    TATGATGTGG CCGAGGCTGA GGAGCACTTC CCGGCGCCCG CGCGGCCGCG CTCACCGTCG
    CCCATCAGCA CGCTGAGCCA CCGCGCGGGC TCGGCCAGCC GCACGGACGA CGATGTGCCG
    TCGCTGCACT CGGAGCCTGT GGCGCGCAGC AGCTCCTCGC AGGGCTCCCT CATGGAGCAG
    ATCAGCAGTG TGGTCACCCG CTTCACGGCC AACATCAGCG AGCTCAACTC CATGATGCTG
    TCCACCGCGG CCCCCAGCCC CGGCGTCGGC GCCCCGCTCT GCTCGTCCTA CCTGATCCCC
    AAAGAGATCC AGTTGCCCAC GACCATGACG ACCTTTGCCG AAATCCAGCC TCTGCCGGCC
    ATCGAAGTCA CGGGAGGCGC GCAGCCCGCG GCAGGGGCGC AGGCGGCTGG GGACGCGGCC
    CGGGAGAGCC CCGCGGCCGG TCCCGAGGCT GCGGCCGCCA AGCCAGACCT GGAGGAGCTG
    GTGGCTCTCA CCCCGCCGTC CCCCTTCAGA GACTCGGTGG ACTCGGGGAG CACAACCCCC
    AACTCGCCAG TGTCCGAGTC GGCCCTCTGT ATCCCGTCGT CTCCCAAATA TGACACTCTT
    ATCATAAGAG ATTACACTCA GAGCTCCTCG TCGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011541094.1
    CDS399..4037
    Translation

    Target ORF information:

    RefSeq Version XM_011542792.1
    Organism Homo sapiens(human)
    Definition Homo sapiens glutamate metabotropic receptor 5 (GRM5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011542792.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG GTACAAAGAC
    AGGAGACTGG CCCAGCACAA GTCGGAAATA GAGTGTTTCA CCCCCAAAGG GAGTATGGGG
    AATGGTGGGA GAGCAACAAT GAGCAGTTCC AATGGAAAAT CCGTCACGTG GGCCCAGAAT
    GAGAAGAGCA GCCGGGGGCA GCACCTGTGG CAGCGCCTGT CCATCCACAT CAACAAGAAA
    GAAAACCCCA ACCAAACGGC CGTCATCAAG CCCTTCCCCA AGAGCACGGA GAGCCGTGGC
    CTGGGCGCTG GCGCTGGCGC AGGCGGGAGC GCTGGGGGCG TGGGGGCCAC GGGCGGTGCG
    GGCTGCGCAG GCGCCGGCCC AGGCGGGCCC GAGTCCCCAG ACGCCGGCCC CAAGGCGCTG
    TATGATGTGG CCGAGGCTGA GGAGCACTTC CCGGCGCCCG CGCGGCCGCG CTCACCGTCG
    CCCATCAGCA CGCTGAGCCA CCGCGCGGGC TCGGCCAGCC GCACGGACGA CGATGTGCCG
    TCGCTGCACT CGGAGCCTGT GGCGCGCAGC AGCTCCTCGC AGGGCTCCCT CATGGAGCAG
    ATCAGCAGTG TGGTCACCCG CTTCACGGCC AACATCAGCG AGCTCAACTC CATGATGCTG
    TCCACCGCGG CCCCCAGCCC CGGCGTCGGC GCCCCGCTCT GCTCGTCCTA CCTGATCCCC
    AAAGAGATCC AGTTGCCCAC GACCATGACG ACCTTTGCCG AAATCCAGCC TCTGCCGGCC
    ATCGAAGTCA CGGGAGGCGC GCAGCCCGCG GCAGGGGCGC AGGCGGCTGG GGACGCGGCC
    CGGGAGAGCC CCGCGGCCGG TCCCGAGGCT GCGGCCGCCA AGCCAGACCT GGAGGAGCTG
    GTGGCTCTCA CCCCGCCGTC CCCCTTCAGA GACTCGGTGG ACTCGGGGAG CACAACCCCC
    AACTCGCCAG TGTCCGAGTC GGCCCTCTGT ATCCCGTCGT CTCCCAAATA TGACACTCTT
    ATCATAAGAG ATTACACTCA GAGCTCCTCG TCGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28022
    Clone ID Related Accession (Same CDS sequence) XM_011542793.1 , XM_011542794.1 , XM_011542792.1 , NM_001143831.2 , XM_006718828.3 , XM_006718828.2 , XM_006718828.4
    Accession Version XM_006718828.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product metabotropic glutamate receptor 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718828.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG GTACAAAGAC
    AGGAGACTGG CCCAGCACAA GTCGGAAATA GAGTGTTTCA CCCCCAAAGG GAGTATGGGG
    AATGGTGGGA GAGCAACAAT GAGCAGTTCC AATGGAAAAT CCGTCACGTG GGCCCAGAAT
    GAGAAGAGCA GCCGGGGGCA GCACCTGTGG CAGCGCCTGT CCATCCACAT CAACAAGAAA
    GAAAACCCCA ACCAAACGGC CGTCATCAAG CCCTTCCCCA AGAGCACGGA GAGCCGTGGC
    CTGGGCGCTG GCGCTGGCGC AGGCGGGAGC GCTGGGGGCG TGGGGGCCAC GGGCGGTGCG
    GGCTGCGCAG GCGCCGGCCC AGGCGGGCCC GAGTCCCCAG ACGCCGGCCC CAAGGCGCTG
    TATGATGTGG CCGAGGCTGA GGAGCACTTC CCGGCGCCCG CGCGGCCGCG CTCACCGTCG
    CCCATCAGCA CGCTGAGCCA CCGCGCGGGC TCGGCCAGCC GCACGGACGA CGATGTGCCG
    TCGCTGCACT CGGAGCCTGT GGCGCGCAGC AGCTCCTCGC AGGGCTCCCT CATGGAGCAG
    ATCAGCAGTG TGGTCACCCG CTTCACGGCC AACATCAGCG AGCTCAACTC CATGATGCTG
    TCCACCGCGG CCCCCAGCCC CGGCGTCGGC GCCCCGCTCT GCTCGTCCTA CCTGATCCCC
    AAAGAGATCC AGTTGCCCAC GACCATGACG ACCTTTGCCG AAATCCAGCC TCTGCCGGCC
    ATCGAAGTCA CGGGAGGCGC GCAGCCCGCG GCAGGGGCGC AGGCGGCTGG GGACGCGGCC
    CGGGAGAGCC CCGCGGCCGG TCCCGAGGCT GCGGCCGCCA AGCCAGACCT GGAGGAGCTG
    GTGGCTCTCA CCCCGCCGTC CCCCTTCAGA GACTCGGTGG ACTCGGGGAG CACAACCCCC
    AACTCGCCAG TGTCCGAGTC GGCCCTCTGT ATCCCGTCGT CTCCCAAATA TGACACTCTT
    ATCATAAGAG ATTACACTCA GAGCTCCTCG TCGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718891.1
    CDS397..4035
    Translation

    Target ORF information:

    RefSeq Version XM_006718828.3
    Organism Homo sapiens(human)
    Definition Homo sapiens glutamate metabotropic receptor 5 (GRM5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718828.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG GTACAAAGAC
    AGGAGACTGG CCCAGCACAA GTCGGAAATA GAGTGTTTCA CCCCCAAAGG GAGTATGGGG
    AATGGTGGGA GAGCAACAAT GAGCAGTTCC AATGGAAAAT CCGTCACGTG GGCCCAGAAT
    GAGAAGAGCA GCCGGGGGCA GCACCTGTGG CAGCGCCTGT CCATCCACAT CAACAAGAAA
    GAAAACCCCA ACCAAACGGC CGTCATCAAG CCCTTCCCCA AGAGCACGGA GAGCCGTGGC
    CTGGGCGCTG GCGCTGGCGC AGGCGGGAGC GCTGGGGGCG TGGGGGCCAC GGGCGGTGCG
    GGCTGCGCAG GCGCCGGCCC AGGCGGGCCC GAGTCCCCAG ACGCCGGCCC CAAGGCGCTG
    TATGATGTGG CCGAGGCTGA GGAGCACTTC CCGGCGCCCG CGCGGCCGCG CTCACCGTCG
    CCCATCAGCA CGCTGAGCCA CCGCGCGGGC TCGGCCAGCC GCACGGACGA CGATGTGCCG
    TCGCTGCACT CGGAGCCTGT GGCGCGCAGC AGCTCCTCGC AGGGCTCCCT CATGGAGCAG
    ATCAGCAGTG TGGTCACCCG CTTCACGGCC AACATCAGCG AGCTCAACTC CATGATGCTG
    TCCACCGCGG CCCCCAGCCC CGGCGTCGGC GCCCCGCTCT GCTCGTCCTA CCTGATCCCC
    AAAGAGATCC AGTTGCCCAC GACCATGACG ACCTTTGCCG AAATCCAGCC TCTGCCGGCC
    ATCGAAGTCA CGGGAGGCGC GCAGCCCGCG GCAGGGGCGC AGGCGGCTGG GGACGCGGCC
    CGGGAGAGCC CCGCGGCCGG TCCCGAGGCT GCGGCCGCCA AGCCAGACCT GGAGGAGCTG
    GTGGCTCTCA CCCCGCCGTC CCCCTTCAGA GACTCGGTGG ACTCGGGGAG CACAACCCCC
    AACTCGCCAG TGTCCGAGTC GGCCCTCTGT ATCCCGTCGT CTCCCAAATA TGACACTCTT
    ATCATAAGAG ATTACACTCA GAGCTCCTCG TCGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28022
    Clone ID Related Accession (Same CDS sequence) XM_011542793.1 , XM_011542794.1 , XM_011542792.1 , NM_001143831.2 , XM_006718828.3 , XM_006718828.2 , XM_006718828.4
    Accession Version XM_006718828.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product metabotropic glutamate receptor 5 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG GTACAAAGAC
    AGGAGACTGG CCCAGCACAA GTCGGAAATA GAGTGTTTCA CCCCCAAAGG GAGTATGGGG
    AATGGTGGGA GAGCAACAAT GAGCAGTTCC AATGGAAAAT CCGTCACGTG GGCCCAGAAT
    GAGAAGAGCA GCCGGGGGCA GCACCTGTGG CAGCGCCTGT CCATCCACAT CAACAAGAAA
    GAAAACCCCA ACCAAACGGC CGTCATCAAG CCCTTCCCCA AGAGCACGGA GAGCCGTGGC
    CTGGGCGCTG GCGCTGGCGC AGGCGGGAGC GCTGGGGGCG TGGGGGCCAC GGGCGGTGCG
    GGCTGCGCAG GCGCCGGCCC AGGCGGGCCC GAGTCCCCAG ACGCCGGCCC CAAGGCGCTG
    TATGATGTGG CCGAGGCTGA GGAGCACTTC CCGGCGCCCG CGCGGCCGCG CTCACCGTCG
    CCCATCAGCA CGCTGAGCCA CCGCGCGGGC TCGGCCAGCC GCACGGACGA CGATGTGCCG
    TCGCTGCACT CGGAGCCTGT GGCGCGCAGC AGCTCCTCGC AGGGCTCCCT CATGGAGCAG
    ATCAGCAGTG TGGTCACCCG CTTCACGGCC AACATCAGCG AGCTCAACTC CATGATGCTG
    TCCACCGCGG CCCCCAGCCC CGGCGTCGGC GCCCCGCTCT GCTCGTCCTA CCTGATCCCC
    AAAGAGATCC AGTTGCCCAC GACCATGACG ACCTTTGCCG AAATCCAGCC TCTGCCGGCC
    ATCGAAGTCA CGGGAGGCGC GCAGCCCGCG GCAGGGGCGC AGGCGGCTGG GGACGCGGCC
    CGGGAGAGCC CCGCGGCCGG TCCCGAGGCT GCGGCCGCCA AGCCAGACCT GGAGGAGCTG
    GTGGCTCTCA CCCCGCCGTC CCCCTTCAGA GACTCGGTGG ACTCGGGGAG CACAACCCCC
    AACTCGCCAG TGTCCGAGTC GGCCCTCTGT ATCCCGTCGT CTCCCAAATA TGACACTCTT
    ATCATAAGAG ATTACACTCA GAGCTCCTCG TCGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718891.1
    Translation

    Target ORF information:

    RefSeq Version XM_006718828.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens glutamate receptor, metabotropic 5 (GRM5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718828.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG GTACAAAGAC
    AGGAGACTGG CCCAGCACAA GTCGGAAATA GAGTGTTTCA CCCCCAAAGG GAGTATGGGG
    AATGGTGGGA GAGCAACAAT GAGCAGTTCC AATGGAAAAT CCGTCACGTG GGCCCAGAAT
    GAGAAGAGCA GCCGGGGGCA GCACCTGTGG CAGCGCCTGT CCATCCACAT CAACAAGAAA
    GAAAACCCCA ACCAAACGGC CGTCATCAAG CCCTTCCCCA AGAGCACGGA GAGCCGTGGC
    CTGGGCGCTG GCGCTGGCGC AGGCGGGAGC GCTGGGGGCG TGGGGGCCAC GGGCGGTGCG
    GGCTGCGCAG GCGCCGGCCC AGGCGGGCCC GAGTCCCCAG ACGCCGGCCC CAAGGCGCTG
    TATGATGTGG CCGAGGCTGA GGAGCACTTC CCGGCGCCCG CGCGGCCGCG CTCACCGTCG
    CCCATCAGCA CGCTGAGCCA CCGCGCGGGC TCGGCCAGCC GCACGGACGA CGATGTGCCG
    TCGCTGCACT CGGAGCCTGT GGCGCGCAGC AGCTCCTCGC AGGGCTCCCT CATGGAGCAG
    ATCAGCAGTG TGGTCACCCG CTTCACGGCC AACATCAGCG AGCTCAACTC CATGATGCTG
    TCCACCGCGG CCCCCAGCCC CGGCGTCGGC GCCCCGCTCT GCTCGTCCTA CCTGATCCCC
    AAAGAGATCC AGTTGCCCAC GACCATGACG ACCTTTGCCG AAATCCAGCC TCTGCCGGCC
    ATCGAAGTCA CGGGAGGCGC GCAGCCCGCG GCAGGGGCGC AGGCGGCTGG GGACGCGGCC
    CGGGAGAGCC CCGCGGCCGG TCCCGAGGCT GCGGCCGCCA AGCCAGACCT GGAGGAGCTG
    GTGGCTCTCA CCCCGCCGTC CCCCTTCAGA GACTCGGTGG ACTCGGGGAG CACAACCCCC
    AACTCGCCAG TGTCCGAGTC GGCCCTCTGT ATCCCGTCGT CTCCCAAATA TGACACTCTT
    ATCATAAGAG ATTACACTCA GAGCTCCTCG TCGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28022
    Clone ID Related Accession (Same CDS sequence) XM_011542793.1 , XM_011542794.1 , XM_011542792.1 , NM_001143831.2 , XM_006718828.3 , XM_006718828.2 , XM_006718828.4
    Accession Version XM_006718828.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product metabotropic glutamate receptor 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006718828.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG GTACAAAGAC
    AGGAGACTGG CCCAGCACAA GTCGGAAATA GAGTGTTTCA CCCCCAAAGG GAGTATGGGG
    AATGGTGGGA GAGCAACAAT GAGCAGTTCC AATGGAAAAT CCGTCACGTG GGCCCAGAAT
    GAGAAGAGCA GCCGGGGGCA GCACCTGTGG CAGCGCCTGT CCATCCACAT CAACAAGAAA
    GAAAACCCCA ACCAAACGGC CGTCATCAAG CCCTTCCCCA AGAGCACGGA GAGCCGTGGC
    CTGGGCGCTG GCGCTGGCGC AGGCGGGAGC GCTGGGGGCG TGGGGGCCAC GGGCGGTGCG
    GGCTGCGCAG GCGCCGGCCC AGGCGGGCCC GAGTCCCCAG ACGCCGGCCC CAAGGCGCTG
    TATGATGTGG CCGAGGCTGA GGAGCACTTC CCGGCGCCCG CGCGGCCGCG CTCACCGTCG
    CCCATCAGCA CGCTGAGCCA CCGCGCGGGC TCGGCCAGCC GCACGGACGA CGATGTGCCG
    TCGCTGCACT CGGAGCCTGT GGCGCGCAGC AGCTCCTCGC AGGGCTCCCT CATGGAGCAG
    ATCAGCAGTG TGGTCACCCG CTTCACGGCC AACATCAGCG AGCTCAACTC CATGATGCTG
    TCCACCGCGG CCCCCAGCCC CGGCGTCGGC GCCCCGCTCT GCTCGTCCTA CCTGATCCCC
    AAAGAGATCC AGTTGCCCAC GACCATGACG ACCTTTGCCG AAATCCAGCC TCTGCCGGCC
    ATCGAAGTCA CGGGAGGCGC GCAGCCCGCG GCAGGGGCGC AGGCGGCTGG GGACGCGGCC
    CGGGAGAGCC CCGCGGCCGG TCCCGAGGCT GCGGCCGCCA AGCCAGACCT GGAGGAGCTG
    GTGGCTCTCA CCCCGCCGTC CCCCTTCAGA GACTCGGTGG ACTCGGGGAG CACAACCCCC
    AACTCGCCAG TGTCCGAGTC GGCCCTCTGT ATCCCGTCGT CTCCCAAATA TGACACTCTT
    ATCATAAGAG ATTACACTCA GAGCTCCTCG TCGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718891.1
    CDS389..4027
    Translation

    Target ORF information:

    RefSeq Version XM_006718828.4
    Organism Homo sapiens(human)
    Definition Homo sapiens glutamate metabotropic receptor 5 (GRM5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718828.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCCTTC TGTTGATCCT GTCAGTCTTA CTTTTGAAAG AAGATGTCCG TGGGAGTGCA 
    CAGTCCAGTG AGAGGAGGGT GGTGGCTCAC ATGCCGGGTG ACATCATTAT TGGAGCTCTC
    TTTTCTGTTC ATCACCAGCC TACTGTGGAC AAAGTTCATG AGAGGAAGTG TGGGGCGGTC
    CGTGAACAGT ATGGCATTCA GAGAGTGGAG GCCATGCTGC ATACCCTGGA AAGGATCAAT
    TCAGACCCCA CACTCTTGCC CAACATCACA CTGGGCTGTG AGATAAGGGA CTCCTGCTGG
    CATTCGGCTG TGGCCCTAGA GCAGAGCATT GAGTTCATAA GAGATTCCCT CATTTCTTCA
    GAAGAGGAAG AAGGCTTGGT ACGCTGTGTG GATGGCTCCT CCTCTTCCTT CCGCTCCAAG
    AAGCCCATAG TAGGGGTCAT TGGGCCTGGC TCCAGTTCTG TAGCCATTCA GGTCCAGAAT
    TTGCTCCAGC TTTTCAACAT ACCTCAGATT GCTTACTCAG CAACCAGCAT GGATCTGAGT
    GACAAGACTC TGTTCAAATA TTTCATGAGG GTTGTGCCTT CAGATGCTCA GCAGGCAAGG
    GCCATGGTGG ACATAGTGAA GAGGTACAAC TGGACCTATG TATCAGCCGT GCACACAGAA
    GGCAACTATG GAGAAAGTGG GATGGAAGCC TTCAAAGATA TGTCAGCGAA GGAAGGGATT
    TGCATCGCCC ACTCTTACAA AATCTACAGT AATGCAGGGG AGCAGAGCTT TGATAAGCTG
    CTGAAGAAGC TCACAAGTCA CTTGCCCAAG GCCCGGGTGG TGGCCTGCTT CTGTGAGGGC
    ATGACGGTGA GAGGTCTGCT GATGGCCATG AGGCGCCTGG GTCTAGCGGG AGAATTTCTG
    CTTCTGGGCA GTGATGGCTG GGCTGACAGG TATGATGTGA CAGATGGATA TCAGCGAGAA
    GCTGTTGGTG GCATCACAAT CAAGCTCCAA TCTCCCGATG TCAAGTGGTT TGATGATTAT
    TATCTGAAGC TCCGGCCAGA AACAAACCAC CGAAACCCTT GGTTTCAAGA ATTTTGGCAG
    CATCGTTTTC AGTGCCGACT GGAAGGGTTT CCACAGGAGA ACAGCAAATA CAACAAGACT
    TGCAATAGTT CTCTGACTCT GAAAACACAT CATGTTCAGG ATTCCAAAAT GGGATTTGTG
    ATCAACGCCA TCTATTCGAT GGCCTATGGG CTCCACAACA TGCAGATGTC CCTCTGCCCA
    GGCTATGCAG GACTCTGTGA TGCCATGAAG CCAATTGATG GACGGAAACT TTTGGAGTCC
    CTGATGAAAA CCAATTTTAC TGGGGTTTCT GGAGATACGA TCCTATTCGA TGAGAATGGA
    GACTCTCCAG GAAGGTATGA AATAATGAAT TTCAAGGAAA TGGGAAAAGA TTACTTTGAT
    TATATCAACG TTGGAAGTTG GGACAATGGA GAATTAAAAA TGGATGATGA TGAAGTATGG
    TCCAAGAAAA GCAACATCAT CAGATCTGTG TGCAGTGAAC CATGTGAGAA AGGCCAGATC
    AAGGTGATCC GAAAGGGAGA AGTCAGCTGT TGTTGGACCT GTACACCTTG TAAGGAGAAT
    GAGTATGTCT TTGATGAGTA CACATGCAAG GCATGCCAAC TGGGGTCTTG GCCCACTGAT
    GATCTCACAG GTTGTGACTT GATCCCAGTA CAGTATCTTC GATGGGGTGA CCCTGAACCC
    ATTGCAGCTG TGGTGTTTGC CTGCCTTGGC CTCCTGGCCA CCCTGTTTGT TACTGTAGTC
    TTCATCATTT ACCGTGATAC ACCAGTAGTC AAGTCCTCAA GCAGGGAACT CTGCTACATT
    ATCCTTGCTG GCATCTGCCT GGGCTACTTA TGTACCTTCT GCCTCATTGC GAAGCCCAAA
    CAGATTTACT GCTACCTTCA GAGAATTGGC ATTGGTCTCT CCCCAGCCAT GAGCTACTCA
    GCCCTTGTAA CAAAGACCAA CCGTATTGCA AGGATCCTGG CTGGCAGCAA GAAGAAGATC
    TGTACCAAAA AGCCCAGATT CATGAGTGCC TGTGCCCAGC TAGTGATTGC TTTCATTCTC
    ATATGCATCC AGTTGGGCAT CATCGTTGCC CTCTTTATAA TGGAGCCTCC TGACATAATG
    CATGACTACC CAAGCATTCG AGAAGTCTAC CTGATCTGTA ACACCACCAA CCTAGGAGTT
    GTCACTCCAC TTGGATACAA TGGATTGTTG ATTTTGAGCT GCACCTTCTA TGCGTTCAAG
    ACCAGAAATG TTCCAGCTAA CTTCAACGAG GCCAAGTATA TCGCCTTCAC AATGTACACG
    ACCTGCATTA TATGGCTAGC TTTTGTGCCA ATCTACTTTG GCAGCAACTA CAAAATCATC
    ACCATGTGTT TCTCGGTCAG CCTCAGTGCC ACAGTGGCCC TAGGCTGCAT GTTTGTGCCG
    AAGGTGTACA TCATCCTGGC CAAACCAGAG AGAAACGTGC GCAGCGCCTT CACCACATCT
    ACCGTGGTGC GCATGCATGT AGGGGATGGC AAGTCATCCT CCGCAGCCAG CAGATCCAGC
    AGCCTAGTCA ACCTGTGGAA GAGAAGGGGC TCCTCTGGGG AAACCTTAAG GTACAAAGAC
    AGGAGACTGG CCCAGCACAA GTCGGAAATA GAGTGTTTCA CCCCCAAAGG GAGTATGGGG
    AATGGTGGGA GAGCAACAAT GAGCAGTTCC AATGGAAAAT CCGTCACGTG GGCCCAGAAT
    GAGAAGAGCA GCCGGGGGCA GCACCTGTGG CAGCGCCTGT CCATCCACAT CAACAAGAAA
    GAAAACCCCA ACCAAACGGC CGTCATCAAG CCCTTCCCCA AGAGCACGGA GAGCCGTGGC
    CTGGGCGCTG GCGCTGGCGC AGGCGGGAGC GCTGGGGGCG TGGGGGCCAC GGGCGGTGCG
    GGCTGCGCAG GCGCCGGCCC AGGCGGGCCC GAGTCCCCAG ACGCCGGCCC CAAGGCGCTG
    TATGATGTGG CCGAGGCTGA GGAGCACTTC CCGGCGCCCG CGCGGCCGCG CTCACCGTCG
    CCCATCAGCA CGCTGAGCCA CCGCGCGGGC TCGGCCAGCC GCACGGACGA CGATGTGCCG
    TCGCTGCACT CGGAGCCTGT GGCGCGCAGC AGCTCCTCGC AGGGCTCCCT CATGGAGCAG
    ATCAGCAGTG TGGTCACCCG CTTCACGGCC AACATCAGCG AGCTCAACTC CATGATGCTG
    TCCACCGCGG CCCCCAGCCC CGGCGTCGGC GCCCCGCTCT GCTCGTCCTA CCTGATCCCC
    AAAGAGATCC AGTTGCCCAC GACCATGACG ACCTTTGCCG AAATCCAGCC TCTGCCGGCC
    ATCGAAGTCA CGGGAGGCGC GCAGCCCGCG GCAGGGGCGC AGGCGGCTGG GGACGCGGCC
    CGGGAGAGCC CCGCGGCCGG TCCCGAGGCT GCGGCCGCCA AGCCAGACCT GGAGGAGCTG
    GTGGCTCTCA CCCCGCCGTC CCCCTTCAGA GACTCGGTGG ACTCGGGGAG CACAACCCCC
    AACTCGCCAG TGTCCGAGTC GGCCCTCTGT ATCCCGTCGT CTCCCAAATA TGACACTCTT
    ATCATAAGAG ATTACACTCA GAGCTCCTCG TCGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.