CD274 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CD274
Entrez Gene ID	29126
Full Name	CD274 molecule
Synonyms	B7-H,B7H1,PD-L1,PDCD1L1,PDCD1LG1,PDL1
General protein information	Preferred Names CD274 molecule Names programmed cell death 1 ligand 1 B7 homolog 1 CD274 antigen PDCD1 ligand 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9p24.1
Summary	This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015].
Disorder MIM:	605402

mRNA and Protein(s)

mRNA	Protein	Name
NM_014143.3	NP_054862.1	programmed cell death 1 ligand 1 isoform a precursor
NM_014143.4	NP_054862.1	programmed cell death 1 ligand 1 isoform a precursor
NM_001314029.1	NP_001300958.1	programmed cell death 1 ligand 1 isoform c precursor
NM_001267706.1	NP_001254635.1	programmed cell death 1 ligand 1 isoform b precursor
NM_001314029.2	NP_001300958.1	programmed cell death 1 ligand 1 isoform c precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CD274	NP_054862.1
Canis lupus familiaris (dog)	CD274	XP_005615991.1
Bos taurus (cattle)	CD274	NP_001156884.1
Mus musculus (house mouse)	Cd274	NP_068693.1
Gallus gallus (chicken)	CD274	XP_424811.4
Pan troglodytes (chimpanzee)	CD274	XP_001140705.1
Rattus norvegicus (Norway rat)	Cd274	NP_001178883.1
Danio rerio (zebrafish)	si:ch211-241b2.5	XP_003200855.2
	CD274	NP_001077358.1
Xenopus tropicalis (tropical clawed frog)	cd274	XP_004911464.1

Gene Ontology
Bibliography

Related articles in PubMed

Oncogenic RAS Signaling Promotes Tumor Immunoresistance by Stabilizing PD-L1 mRNA.
Coelho MA, de Carn? Tr?cesson S, Rana S, Zecchin D, Moore C, Molina-Arcas M, East P, Spencer-Dene B, Nye E, Barnouin K, Snijders AP, Lai WS, Blackshear PJ, Downward J
Immunity47(6)1083-1099.e6(2017 Dec)

Structure and interactions of the human programmed cell death 1 receptor.
Cheng X, Veverka V, Radhakrishnan A, Waters LC, Muskett FW, Morgan SH, Huo J, Yu C, Evans EJ, Leslie AJ, Griffiths M, Stubberfield C, Griffin R, Henry AJ, Jansson A, Ladbury JE, Ikemizu S, Carr MD, Davis SJ
The Journal of biological chemistry288(17)11771-85(2013 Apr)

Regulatory T cells and human myeloid dendritic cells promote tolerance via programmed death ligand-1.
Amarnath S, Costanzo CM, Mariotti J, Ullman JL, Telford WG, Kapoor V, Riley JL, Levine BL, June CH, Fong T, Warner NL, Fowler DH
PLoS biology8(2)e1000302(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Collectively, our findings indicate that miR-140 exerts anti-OS efficacy by targeting immune checkpoint molecule PD-L1 and can be developed as a novel immunotherapeutic agent for the treatment of OS.
Title: MicroRNA-140 suppresses osteosarcoma tumor growth by enhancing anti-tumor immune response and blocking mTOR signaling.

Quantification of PD-L1 expression is feasible on cytology specimens, and the results are comparable to those obtained from surgical resection and small biopsy specimens, including in matched specimens and using a single predictive Immunohistochemistry marker.
Title: PD-L1 expression in non-small cell lung carcinoma: Comparison among cytology, small biopsy, and surgical resection specimens.

conformation and ligand binding analysis of PD-L1
Title: The Too Many Faces of PD-L1: A Comprehensive Conformational Analysis Study.

Suggest of the positive conversion of PD-L1 expression after treatment including nivolumab and that PD-L1 expression must be assessed in not only the resected specimens, but also in the re-biopsied ones.
Title: Positive Conversion of PD-L1 Expression After Treatments with Chemotherapy and Nivolumab.

Data show that programmed death ligand 1 (PD-L1) is expressed on brown adipocytes, does not change upon brown adipose tissue (BAT) activation.
Title: PD-L1 is an activation-independent marker of brown adipocytes.

The following CD274 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD274 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22144	NM_014143.4 Latest version!	Homo sapiens CD274 molecule (CD274), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu22180	NM_001267706.1 Latest version!	Homo sapiens CD274 molecule (CD274), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu101691	NM_001314029.2 Latest version!	Homo sapiens CD274 molecule (CD274), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu22144	NM_014143.3	Homo sapiens CD274 molecule (CD274), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu101691	NM_001314029.1	Homo sapiens CD274 molecule (CD274), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CD274 ( NM_014143.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_054862.1 Homo sapiens CD274 molecule (CD274), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22144
Clone ID Related Accession (Same CDS sequence)	NM_014143.3 , NM_014143.4
Accession Version	NM_014143.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	programmed cell death 1 ligand 1 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL135786.17, AK314567.1, AL162253.17 and AI826502.1. On Mar 31, 2010 this sequence version replaced NM_014143.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.237105.1, SRR1803614.31465.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGATAT TTGCTGTCTT TATATTCATG ACCTACTGGC ATTTGCTGAA CGCATTTACT 
GTCACGGTTC CCAAGGACCT ATATGTGGTA GAGTATGGTA GCAATATGAC AATTGAATGC 
AAATTCCCAG TAGAAAAACA ATTAGACCTG GCTGCACTAA TTGTCTATTG GGAAATGGAG 
GATAAGAACA TTATTCAATT TGTGCATGGA GAGGAAGACC TGAAGGTTCA GCATAGTAGC 
TACAGACAGA GGGCCCGGCT GTTGAAGGAC CAGCTCTCCC TGGGAAATGC TGCACTTCAG 
ATCACAGATG TGAAATTGCA GGATGCAGGG GTGTACCGCT GCATGATCAG CTATGGTGGT 
GCCGACTACA AGCGAATTAC TGTGAAAGTC AATGCCCCAT ACAACAAAAT CAACCAAAGA 
ATTTTGGTTG TGGATCCAGT CACCTCTGAA CATGAACTGA CATGTCAGGC TGAGGGCTAC 
CCCAAGGCCG AAGTCATCTG GACAAGCAGT GACCATCAAG TCCTGAGTGG TAAGACCACC 
ACCACCAATT CCAAGAGAGA GGAGAAGCTT TTCAATGTGA CCAGCACACT GAGAATCAAC 
ACAACAACTA ATGAGATTTT CTACTGCACT TTTAGGAGAT TAGATCCTGA GGAAAACCAT 
ACAGCTGAAT TGGTCATCCC AGAACTACCT CTGGCACATC CTCCAAATGA AAGGACTCAC 
TTGGTAATTC TGGGAGCCAT CTTATTATGC CTTGGTGTAG CACTGACATT CATCTTCCGT 
TTAAGAAAAG GGAGAATGAT GGATGTGAAA AAATGTGGCA TCCAAGATAC AAACTCAAAG 
AAGCAAAGTG ATACACATTT GGAGGAGACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054862.1
CDS	109..981
Translation	MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEET

Target ORF information:

RefSeq Version	NM_014143.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CD274 molecule (CD274), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014143.3

ORF Insert Sequence:

ATGAGGATAT TTGCTGTCTT TATATTCATG ACCTACTGGC ATTTGCTGAA CGCATTTACT 
GTCACGGTTC CCAAGGACCT ATATGTGGTA GAGTATGGTA GCAATATGAC AATTGAATGC 
AAATTCCCAG TAGAAAAACA ATTAGACCTG GCTGCACTAA TTGTCTATTG GGAAATGGAG 
GATAAGAACA TTATTCAATT TGTGCATGGA GAGGAAGACC TGAAGGTTCA GCATAGTAGC 
TACAGACAGA GGGCCCGGCT GTTGAAGGAC CAGCTCTCCC TGGGAAATGC TGCACTTCAG 
ATCACAGATG TGAAATTGCA GGATGCAGGG GTGTACCGCT GCATGATCAG CTATGGTGGT 
GCCGACTACA AGCGAATTAC TGTGAAAGTC AATGCCCCAT ACAACAAAAT CAACCAAAGA 
ATTTTGGTTG TGGATCCAGT CACCTCTGAA CATGAACTGA CATGTCAGGC TGAGGGCTAC 
CCCAAGGCCG AAGTCATCTG GACAAGCAGT GACCATCAAG TCCTGAGTGG TAAGACCACC 
ACCACCAATT CCAAGAGAGA GGAGAAGCTT TTCAATGTGA CCAGCACACT GAGAATCAAC 
ACAACAACTA ATGAGATTTT CTACTGCACT TTTAGGAGAT TAGATCCTGA GGAAAACCAT 
ACAGCTGAAT TGGTCATCCC AGAACTACCT CTGGCACATC CTCCAAATGA AAGGACTCAC 
TTGGTAATTC TGGGAGCCAT CTTATTATGC CTTGGTGTAG CACTGACATT CATCTTCCGT 
TTAAGAAAAG GGAGAATGAT GGATGTGAAA AAATGTGGCA TCCAAGATAC AAACTCAAAG 
AAGCAAAGTG ATACACATTT GGAGGAGACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD274 ( NM_014143.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_054862.1 Homo sapiens CD274 molecule (CD274), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22144
Clone ID Related Accession (Same CDS sequence)	NM_014143.3 , NM_014143.4
Accession Version	NM_014143.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	programmed cell death 1 ligand 1 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314567.1, AL162253.17 and AI826502.1. On Nov 22, 2018 this sequence version replaced NM_014143.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314567.1, SRR1660803.237105.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000381577.4/ ENSP00000370989.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGATAT TTGCTGTCTT TATATTCATG ACCTACTGGC ATTTGCTGAA CGCATTTACT 
GTCACGGTTC CCAAGGACCT ATATGTGGTA GAGTATGGTA GCAATATGAC AATTGAATGC 
AAATTCCCAG TAGAAAAACA ATTAGACCTG GCTGCACTAA TTGTCTATTG GGAAATGGAG 
GATAAGAACA TTATTCAATT TGTGCATGGA GAGGAAGACC TGAAGGTTCA GCATAGTAGC 
TACAGACAGA GGGCCCGGCT GTTGAAGGAC CAGCTCTCCC TGGGAAATGC TGCACTTCAG 
ATCACAGATG TGAAATTGCA GGATGCAGGG GTGTACCGCT GCATGATCAG CTATGGTGGT 
GCCGACTACA AGCGAATTAC TGTGAAAGTC AATGCCCCAT ACAACAAAAT CAACCAAAGA 
ATTTTGGTTG TGGATCCAGT CACCTCTGAA CATGAACTGA CATGTCAGGC TGAGGGCTAC 
CCCAAGGCCG AAGTCATCTG GACAAGCAGT GACCATCAAG TCCTGAGTGG TAAGACCACC 
ACCACCAATT CCAAGAGAGA GGAGAAGCTT TTCAATGTGA CCAGCACACT GAGAATCAAC 
ACAACAACTA ATGAGATTTT CTACTGCACT TTTAGGAGAT TAGATCCTGA GGAAAACCAT 
ACAGCTGAAT TGGTCATCCC AGAACTACCT CTGGCACATC CTCCAAATGA AAGGACTCAC 
TTGGTAATTC TGGGAGCCAT CTTATTATGC CTTGGTGTAG CACTGACATT CATCTTCCGT 
TTAAGAAAAG GGAGAATGAT GGATGTGAAA AAATGTGGCA TCCAAGATAC AAACTCAAAG 
AAGCAAAGTG ATACACATTT GGAGGAGACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054862.1
CDS	70..942
Translation	MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEET

Target ORF information:

RefSeq Version	NM_014143.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CD274 molecule (CD274), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014143.4

ORF Insert Sequence:

ATGAGGATAT TTGCTGTCTT TATATTCATG ACCTACTGGC ATTTGCTGAA CGCATTTACT 
GTCACGGTTC CCAAGGACCT ATATGTGGTA GAGTATGGTA GCAATATGAC AATTGAATGC 
AAATTCCCAG TAGAAAAACA ATTAGACCTG GCTGCACTAA TTGTCTATTG GGAAATGGAG 
GATAAGAACA TTATTCAATT TGTGCATGGA GAGGAAGACC TGAAGGTTCA GCATAGTAGC 
TACAGACAGA GGGCCCGGCT GTTGAAGGAC CAGCTCTCCC TGGGAAATGC TGCACTTCAG 
ATCACAGATG TGAAATTGCA GGATGCAGGG GTGTACCGCT GCATGATCAG CTATGGTGGT 
GCCGACTACA AGCGAATTAC TGTGAAAGTC AATGCCCCAT ACAACAAAAT CAACCAAAGA 
ATTTTGGTTG TGGATCCAGT CACCTCTGAA CATGAACTGA CATGTCAGGC TGAGGGCTAC 
CCCAAGGCCG AAGTCATCTG GACAAGCAGT GACCATCAAG TCCTGAGTGG TAAGACCACC 
ACCACCAATT CCAAGAGAGA GGAGAAGCTT TTCAATGTGA CCAGCACACT GAGAATCAAC 
ACAACAACTA ATGAGATTTT CTACTGCACT TTTAGGAGAT TAGATCCTGA GGAAAACCAT 
ACAGCTGAAT TGGTCATCCC AGAACTACCT CTGGCACATC CTCCAAATGA AAGGACTCAC 
TTGGTAATTC TGGGAGCCAT CTTATTATGC CTTGGTGTAG CACTGACATT CATCTTCCGT 
TTAAGAAAAG GGAGAATGAT GGATGTGAAA AAATGTGGCA TCCAAGATAC AAACTCAAAG 
AAGCAAAGTG ATACACATTT GGAGGAGACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD274 ( NM_001267706.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001254635.1 Homo sapiens CD274 molecule (CD274), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22180
Clone ID Related Accession (Same CDS sequence)	NM_001267706.1
Accession Version	NM_001267706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Homo sapiens(human)
Product	programmed cell death 1 ligand 1 isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL135786.17, DQ836393.1, AY291313.1, AL162253.17 and AI826502.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform b), compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY291313.1, DQ286582.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGATAT TTGCTGTCTT TATATTCATG ACCTACTGGC ATTTGCTGAA CGCCCCATAC 
AACAAAATCA ACCAAAGAAT TTTGGTTGTG GATCCAGTCA CCTCTGAACA TGAACTGACA 
TGTCAGGCTG AGGGCTACCC CAAGGCCGAA GTCATCTGGA CAAGCAGTGA CCATCAAGTC 
CTGAGTGGTA AGACCACCAC CACCAATTCC AAGAGAGAGG AGAAGCTTTT CAATGTGACC 
AGCACACTGA GAATCAACAC AACAACTAAT GAGATTTTCT ACTGCACTTT TAGGAGATTA 
GATCCTGAGG AAAACCATAC AGCTGAATTG GTCATCCCAG AACTACCTCT GGCACATCCT 
CCAAATGAAA GGACTCACTT GGTAATTCTG GGAGCCATCT TATTATGCCT TGGTGTAGCA 
CTGACATTCA TCTTCCGTTT AAGAAAAGGG AGAATGATGG ATGTGAAAAA ATGTGGCATC 
CAAGATACAA ACTCAAAGAA GCAAAGTGAT ACACATTTGG AGGAGACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254635.1
CDS	109..639
Translation	MRIFAVFIFMTYWHLLNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEET

Target ORF information:

RefSeq Version	NM_001267706.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CD274 molecule (CD274), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267706.1

ORF Insert Sequence:

ATGAGGATAT TTGCTGTCTT TATATTCATG ACCTACTGGC ATTTGCTGAA CGCCCCATAC 
AACAAAATCA ACCAAAGAAT TTTGGTTGTG GATCCAGTCA CCTCTGAACA TGAACTGACA 
TGTCAGGCTG AGGGCTACCC CAAGGCCGAA GTCATCTGGA CAAGCAGTGA CCATCAAGTC 
CTGAGTGGTA AGACCACCAC CACCAATTCC AAGAGAGAGG AGAAGCTTTT CAATGTGACC 
AGCACACTGA GAATCAACAC AACAACTAAT GAGATTTTCT ACTGCACTTT TAGGAGATTA 
GATCCTGAGG AAAACCATAC AGCTGAATTG GTCATCCCAG AACTACCTCT GGCACATCCT 
CCAAATGAAA GGACTCACTT GGTAATTCTG GGAGCCATCT TATTATGCCT TGGTGTAGCA 
CTGACATTCA TCTTCCGTTT AAGAAAAGGG AGAATGATGG ATGTGAAAAA ATGTGGCATC 
CAAGATACAA ACTCAAAGAA GCAAAGTGAT ACACATTTGG AGGAGACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD274 ( NM_001314029.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001300958.1 Homo sapiens CD274 molecule (CD274), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu101691
Clone ID Related Accession (Same CDS sequence)	NM_001314029.1 , NM_001314029.2
Accession Version	NM_001314029.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	programmed cell death 1 ligand 1 isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL135786.17, DQ836393.1, DB160805.1 and AA399416.1. Transcript Variant: This variant (4) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and novel 3' UTR compared to variant 1. It encodes isoform c which is shorter than and has a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA218876.1, DA225575.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGATAT TTGCTGTCTT TATATTCATG ACCTACTGGC ATTTGCTGAA CGCATTTACT 
GTCACGGTTC CCAAGGACCT ATATGTGGTA GAGTATGGTA GCAATATGAC AATTGAATGC 
AAATTCCCAG TAGAAAAACA ATTAGACCTG GCTGCACTAA TTGTCTATTG GGAAATGGAG 
GATAAGAACA TTATTCAATT TGTGCATGGA GAGGAAGACC TGAAGGTTCA GCATAGTAGC 
TACAGACAGA GGGCCCGGCT GTTGAAGGAC CAGCTCTCCC TGGGAAATGC TGCACTTCAG 
ATCACAGATG TGAAATTGCA GGATGCAGGG GTGTACCGCT GCATGATCAG CTATGGTGGT 
GCCGACTACA AGCGAATTAC TGTGAAAGTC AATGCCCCAT ACAACAAAAT CAACCAAAGA 
ATTTTGGTTG TGGATCCAGT CACCTCTGAA CATGAACTGA CATGTCAGGC TGAGGGCTAC 
CCCAAGGCCG AAGTCATCTG GACAAGCAGT GACCATCAAG TCCTGAGTGG TAAGACCACC 
ACCACCAATT CCAAGAGAGA GGAGAAGCTT TTCAATGTGA CCAGCACACT GAGAATCAAC 
ACAACAACTA ATGAGATTTT CTACTGCACT TTTAGGAGAT TAGATCCTGA GGAAAACCAT 
ACAGCTGAAT TGGTCATCCC AGGTAATATT CTGAATGTGT CCATTAAAAT ATGTCTAACA 
CTGTCCCCTA GCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300958.1
CDS	109..846
Translation	MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPGNILNVSIKICLTLSPST

Target ORF information:

RefSeq Version	NM_001314029.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CD274 molecule (CD274), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001314029.1

ORF Insert Sequence:

ATGAGGATAT TTGCTGTCTT TATATTCATG ACCTACTGGC ATTTGCTGAA CGCATTTACT 
GTCACGGTTC CCAAGGACCT ATATGTGGTA GAGTATGGTA GCAATATGAC AATTGAATGC 
AAATTCCCAG TAGAAAAACA ATTAGACCTG GCTGCACTAA TTGTCTATTG GGAAATGGAG 
GATAAGAACA TTATTCAATT TGTGCATGGA GAGGAAGACC TGAAGGTTCA GCATAGTAGC 
TACAGACAGA GGGCCCGGCT GTTGAAGGAC CAGCTCTCCC TGGGAAATGC TGCACTTCAG 
ATCACAGATG TGAAATTGCA GGATGCAGGG GTGTACCGCT GCATGATCAG CTATGGTGGT 
GCCGACTACA AGCGAATTAC TGTGAAAGTC AATGCCCCAT ACAACAAAAT CAACCAAAGA 
ATTTTGGTTG TGGATCCAGT CACCTCTGAA CATGAACTGA CATGTCAGGC TGAGGGCTAC 
CCCAAGGCCG AAGTCATCTG GACAAGCAGT GACCATCAAG TCCTGAGTGG TAAGACCACC 
ACCACCAATT CCAAGAGAGA GGAGAAGCTT TTCAATGTGA CCAGCACACT GAGAATCAAC 
ACAACAACTA ATGAGATTTT CTACTGCACT TTTAGGAGAT TAGATCCTGA GGAAAACCAT 
ACAGCTGAAT TGGTCATCCC AGGTAATATT CTGAATGTGT CCATTAAAAT ATGTCTAACA 
CTGTCCCCTA GCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD274 ( NM_001314029.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001300958.1 Homo sapiens CD274 molecule (CD274), transcript variant 4, mRNA.

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CloneID	OHu101691
Clone ID Related Accession (Same CDS sequence)	NM_001314029.1 , NM_001314029.2
Accession Version	NM_001314029.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	programmed cell death 1 ligand 1 isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ836393.1, DB160805.1 and AA399416.1. On May 31, 2019 this sequence version replaced NM_001314029.1. Transcript Variant: This variant (4) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and novel 3' UTR compared to variant 1. It encodes isoform c which is shorter than and has a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189658.39711.1, DA225575.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGATAT TTGCTGTCTT TATATTCATG ACCTACTGGC ATTTGCTGAA CGCATTTACT 
GTCACGGTTC CCAAGGACCT ATATGTGGTA GAGTATGGTA GCAATATGAC AATTGAATGC 
AAATTCCCAG TAGAAAAACA ATTAGACCTG GCTGCACTAA TTGTCTATTG GGAAATGGAG 
GATAAGAACA TTATTCAATT TGTGCATGGA GAGGAAGACC TGAAGGTTCA GCATAGTAGC 
TACAGACAGA GGGCCCGGCT GTTGAAGGAC CAGCTCTCCC TGGGAAATGC TGCACTTCAG 
ATCACAGATG TGAAATTGCA GGATGCAGGG GTGTACCGCT GCATGATCAG CTATGGTGGT 
GCCGACTACA AGCGAATTAC TGTGAAAGTC AATGCCCCAT ACAACAAAAT CAACCAAAGA 
ATTTTGGTTG TGGATCCAGT CACCTCTGAA CATGAACTGA CATGTCAGGC TGAGGGCTAC 
CCCAAGGCCG AAGTCATCTG GACAAGCAGT GACCATCAAG TCCTGAGTGG TAAGACCACC 
ACCACCAATT CCAAGAGAGA GGAGAAGCTT TTCAATGTGA CCAGCACACT GAGAATCAAC 
ACAACAACTA ATGAGATTTT CTACTGCACT TTTAGGAGAT TAGATCCTGA GGAAAACCAT 
ACAGCTGAAT TGGTCATCCC AGGTAATATT CTGAATGTGT CCATTAAAAT ATGTCTAACA 
CTGTCCCCTA GCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300958.1
CDS	70..807
Translation	MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPGNILNVSIKICLTLSPST

Target ORF information:

RefSeq Version	NM_001314029.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CD274 molecule (CD274), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001314029.2

ORF Insert Sequence:

ATGAGGATAT TTGCTGTCTT TATATTCATG ACCTACTGGC ATTTGCTGAA CGCATTTACT 
GTCACGGTTC CCAAGGACCT ATATGTGGTA GAGTATGGTA GCAATATGAC AATTGAATGC 
AAATTCCCAG TAGAAAAACA ATTAGACCTG GCTGCACTAA TTGTCTATTG GGAAATGGAG 
GATAAGAACA TTATTCAATT TGTGCATGGA GAGGAAGACC TGAAGGTTCA GCATAGTAGC 
TACAGACAGA GGGCCCGGCT GTTGAAGGAC CAGCTCTCCC TGGGAAATGC TGCACTTCAG 
ATCACAGATG TGAAATTGCA GGATGCAGGG GTGTACCGCT GCATGATCAG CTATGGTGGT 
GCCGACTACA AGCGAATTAC TGTGAAAGTC AATGCCCCAT ACAACAAAAT CAACCAAAGA 
ATTTTGGTTG TGGATCCAGT CACCTCTGAA CATGAACTGA CATGTCAGGC TGAGGGCTAC 
CCCAAGGCCG AAGTCATCTG GACAAGCAGT GACCATCAAG TCCTGAGTGG TAAGACCACC 
ACCACCAATT CCAAGAGAGA GGAGAAGCTT TTCAATGTGA CCAGCACACT GAGAATCAAC 
ACAACAACTA ATGAGATTTT CTACTGCACT TTTAGGAGAT TAGATCCTGA GGAAAACCAT 
ACAGCTGAAT TGGTCATCCC AGGTAATATT CTGAATGTGT CCATTAAAAT ATGTCTAACA 
CTGTCCCCTA GCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Oncogenic RAS Signaling Promotes Tumor Immunoresistance by Stabilizing PD-L1 mRNA.
Immunity47(6)1083-1099.e6(2017 Dec)
Coelho MA,de Carn? Tr?cesson S,Rana S,Zecchin D,Moore C,Molina-Arcas M,East P,Spencer-Dene B,Nye E,Barnouin K,Snijders AP,Lai WS,Blackshear PJ,Downward J

Structure and interactions of the human programmed cell death 1 receptor.
The Journal of biological chemistry288(17)11771-85(2013 Apr)
Cheng X,Veverka V,Radhakrishnan A,Waters LC,Muskett FW,Morgan SH,Huo J,Yu C,Evans EJ,Leslie AJ,Griffiths M,Stubberfield C,Griffin R,Henry AJ,Jansson A,Ladbury JE,Ikemizu S,Carr MD,Davis SJ

Regulatory T cells and human myeloid dendritic cells promote tolerance via programmed death ligand-1.
PLoS biology8(2)e1000302(2010 Feb)
Amarnath S,Costanzo CM,Mariotti J,Ullman JL,Telford WG,Kapoor V,Riley JL,Levine BL,June CH,Fong T,Warner NL,Fowler DH

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CD274 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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