SSU72 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SSU72
Entrez Gene ID	29101
Full Name	SSU72 homolog, RNA polymerase II CTD phosphatase
Synonyms	HSPC182,PNAS-120
General protein information	Preferred Names SSU72 homolog, RNA polymerase II CTD phosphatase Names RNA polymerase II subunit A C-terminal domain phosphatase SSU72 CTD phosphatase SSU72
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.33
Disorder MIM:	617680

mRNA and Protein(s)

mRNA	Protein	Name
NM_014188.2	NP_054907.1	RNA polymerase II subunit A C-terminal domain phosphatase SSU72
NM_014188.3	NP_054907.1	RNA polymerase II subunit A C-terminal domain phosphatase SSU72

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ssu72	NP_957022.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011470	XP_317836.3
Schizosaccharomyces pombe (fission yeast)	ssu72	NP_594076.1
Oryza sativa (rice)	Os12g0168200	NP_001066253.1
Xenopus tropicalis (tropical clawed frog)	ssu72	NP_001016264.1
	SSU72	XP_001094964.1
Mus musculus (house mouse)	Ssu72	NP_081175.2
Gallus gallus (chicken)	SSU72	NP_001007876.1
Drosophila melanogaster (fruit fly)	Ssu72	NP_608342.1
Caenorhabditis elegans (roundworm)	ssup-72	NP_495386.3
Kluyveromyces lactis	KLLA0D19514g	XP_453928.1
Bos taurus (cattle)	SSU72	NP_001069817.1
Homo sapiens (human)	SSU72	NP_054907.1
Canis lupus familiaris (dog)	SSU72	NP_001239254.1
Eremothecium gossypii	AGOS_ABL190W	NP_982757.1
Magnaporthe oryzae (rice blast fungus)	MGG_03704	XP_003716177.1
Neurospora crassa	NCU03114	XP_964973.1
Arabidopsis thaliana (thale cress)	AT1G73820	NP_177523.1
Pan troglodytes (chimpanzee)	SSU72	XP_001147261.1
Rattus norvegicus (Norway rat)	Ssu72	NP_001020828.1
Saccharomyces cerevisiae (baker's yeast)	SSU72	NP_014177.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural dissection of an interaction between transcription initiation and termination factors implicated in promoter-terminator crosstalk.
Bratkowski M, Unarta IC, Zhu L, Shubbar M, Huang X, Liu X
The Journal of biological chemistry(2017 Nov)

Functional interplay between Aurora B kinase and Ssu72 phosphatase regulates sister chromatid cohesion.
Kim HS, Kim SH, Park HY, Lee J, Yoon JH, Choi S, Ryu SH, Lee H, Cho HS, Lee CW
Nature communications42631(2013)

An unexpected binding mode for a Pol II CTD peptide phosphorylated at Ser7 in the active site of the CTD phosphatase Ssu72.
Xiang K, Manley JL, Tong L
Genes & development26(20)2265-70(2012 Oct)

Ssu72 phosphatase-dependent erasure of phospho-Ser7 marks on the RNA polymerase II C-terminal domain is essential for viability and transcription termination.
Zhang DW, Mosley AL, Ramisetty SR, Rodr?guez-Molina JB, Washburn MP, Ansari AZ
The Journal of biological chemistry287(11)8541-51(2012 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Aurora B kinase directly targets Ssu72 phosphatase for regulation of sister chromatid cohesion during early mitosis.
Title: Functional interplay between Aurora B kinase and Ssu72 phosphatase regulates sister chromatid cohesion.

The pSer7 phosphatase activity of Ssu72 is approximately 4000-fold lower than its pSer5 phosphatase activity toward a peptide substrate
Title: An unexpected binding mode for a Pol II CTD peptide phosphorylated at Ser7 in the active site of the CTD phosphatase Ssu72.

Data suggest that Ssu72 is a novel cohesin-binding protein capable of regulating cohesion between sister chromatid arms.
Title: The hsSsu72 phosphatase is a cohesin-binding protein that regulates the resolution of sister chromatid arm cohesion.

crystal structure at 2.4 A resolution of the amino-terminal domain (residues 30-340) of human symplekin in a ternary complex with the Pol II carboxy-terminal domain (CTD) Ser 5 phosphatase Ssu72 and a CTD Ser 5 phosphopeptide
Title: Crystal structure of the human symplekin-Ssu72-CTD phosphopeptide complex.

Observational study of gene-disease association. (HuGE Navigator)
Title: High resolution linkage and linkage disequilibrium analyses of chromosome 1p36 SNPs identify new positional candidate genes for low bone mineral density.

The following SSU72 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SSU72 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19936	NM_014188.3 Latest version!	Homo sapiens SSU72 homolog, RNA polymerase II CTD phosphatase (SSU72), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu19936	NM_014188.2	Homo sapiens SSU72 homolog, RNA polymerase II CTD phosphatase (SSU72), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SSU72 ( NM_014188.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_054907.1 Homo sapiens SSU72 homolog, RNA polymerase II CTD phosphatase (SSU72), mRNA.

Price & Availability↑

CloneID	OHu19936
Clone ID Related Accession (Same CDS sequence)	NM_014188.2 , NM_014188.3
Accession Version	NM_014188.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-20
Organism	Homo sapiens(human)
Product	RNA polymerase II subunit A C-terminal domain phosphatase SSU72
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF594026.1, AJ276409.1 and AF161530.1. On Apr 14, 2004 this sequence version replaced NM_014188.1. ##Evidence-Data-START## Transcript exon combination :: BC018909.2, SRR1163657.401257.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGTCGT CCCCGCTGCG GGTGGCGGTG GTGTGCTCGA GCAACCAGAA CCGGAGCATG 
GAGGCGCACA ACATCCTCAG CAAACGGGGA TTCAGCGTCC GATCCTTTGG AACAGGGACT 
CACGTGAAGC TTCCAGGACC AGCTCCCGAC AAGCCCAATG TTTATGATTT CAAAACCACA 
TATGACCAGA TGTACAATGA TCTTCTTAGG AAAGACAAAG AACTCTATAC ACAGAATGGG 
ATTTTACATA TGCTGGACAG AAATAAGAGA ATCAAGCCCC GGCCAGAAAG ATTCCAGAAC 
TGCAAAGACC TGTTTGATCT GATCCTCACT TGCGAAGAGA GAGTGTATGA CCAGGTGGTG 
GAAGATCTGA ATTCCAGAGA ACAGGAGACC TGCCAGCCTG TGCACGTGGT CAATGTGGAC 
ATCCAGGACA ACCACGAGGA GGCCACCCTG GGGGCGTTTC TCATCTGTGA GCTCTGCCAG 
TGTATCCAGC ACACGGAAGA CATGGAGAAC GAGATCGACG AGCTGCTGCA GGAGTTCGAG 
GAGAAGAGTG GCCGCACCTT TCTGCACACC GTCTGCTTCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054907.1
CDS	326..910
Translation	MPSSPLRVAVVCSSNQNRSMEAHNILSKRGFSVRSFGTGTHVKLPGPAPDKPNVYDFKTTYDQMYNDLLRKDKELYTQNGILHMLDRNKRIKPRPERFQNCKDLFDLILTCEERVYDQVVEDLNSREQETCQPVHVVNVDIQDNHEEATLGAFLICELCQCIQHTEDMENEIDELLQEFEEKSGRTFLHTVCFY

Target ORF information:

RefSeq Version	NM_014188.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SSU72 homolog, RNA polymerase II CTD phosphatase (SSU72), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014188.2

ORF Insert Sequence:

ATGCCGTCGT CCCCGCTGCG GGTGGCGGTG GTGTGCTCGA GCAACCAGAA CCGGAGCATG 
GAGGCGCACA ACATCCTCAG CAAACGGGGA TTCAGCGTCC GATCCTTTGG AACAGGGACT 
CACGTGAAGC TTCCAGGACC AGCTCCCGAC AAGCCCAATG TTTATGATTT CAAAACCACA 
TATGACCAGA TGTACAATGA TCTTCTTAGG AAAGACAAAG AACTCTATAC ACAGAATGGG 
ATTTTACATA TGCTGGACAG AAATAAGAGA ATCAAGCCCC GGCCAGAAAG ATTCCAGAAC 
TGCAAAGACC TGTTTGATCT GATCCTCACT TGCGAAGAGA GAGTGTATGA CCAGGTGGTG 
GAAGATCTGA ATTCCAGAGA ACAGGAGACC TGCCAGCCTG TGCACGTGGT CAATGTGGAC 
ATCCAGGACA ACCACGAGGA GGCCACCCTG GGGGCGTTTC TCATCTGTGA GCTCTGCCAG 
TGTATCCAGC ACACGGAAGA CATGGAGAAC GAGATCGACG AGCTGCTGCA GGAGTTCGAG 
GAGAAGAGTG GCCGCACCTT TCTGCACACC GTCTGCTTCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSU72 ( NM_014188.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_054907.1 Homo sapiens SSU72 homolog, RNA polymerase II CTD phosphatase (SSU72), mRNA.

Price & Availability↑

CloneID	OHu19936
Clone ID Related Accession (Same CDS sequence)	NM_014188.2 , NM_014188.3
Accession Version	NM_014188.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	RNA polymerase II subunit A C-terminal domain phosphatase SSU72
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF594026.1, AJ276409.1 and AF161530.1. On Nov 23, 2018 this sequence version replaced NM_014188.2. ##Evidence-Data-START## Transcript exon combination :: BC018909.2, SRR1163657.401257.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000291386.4/ ENSP00000291386.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGTCGT CCCCGCTGCG GGTGGCGGTG GTGTGCTCGA GCAACCAGAA CCGGAGCATG 
GAGGCGCACA ACATCCTCAG CAAACGGGGA TTCAGCGTCC GATCCTTTGG AACAGGGACT 
CACGTGAAGC TTCCAGGACC AGCTCCCGAC AAGCCCAATG TTTATGATTT CAAAACCACA 
TATGACCAGA TGTACAATGA TCTTCTTAGG AAAGACAAAG AACTCTATAC ACAGAATGGG 
ATTTTACATA TGCTGGACAG AAATAAGAGA ATCAAGCCCC GGCCAGAAAG ATTCCAGAAC 
TGCAAAGACC TGTTTGATCT GATCCTCACT TGCGAAGAGA GAGTGTATGA CCAGGTGGTG 
GAAGATCTGA ATTCCAGAGA ACAGGAGACC TGCCAGCCTG TGCACGTGGT CAATGTGGAC 
ATCCAGGACA ACCACGAGGA GGCCACCCTG GGGGCGTTTC TCATCTGTGA GCTCTGCCAG 
TGTATCCAGC ACACGGAAGA CATGGAGAAC GAGATCGACG AGCTGCTGCA GGAGTTCGAG 
GAGAAGAGTG GCCGCACCTT TCTGCACACC GTCTGCTTCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054907.1
CDS	307..891
Translation	MPSSPLRVAVVCSSNQNRSMEAHNILSKRGFSVRSFGTGTHVKLPGPAPDKPNVYDFKTTYDQMYNDLLRKDKELYTQNGILHMLDRNKRIKPRPERFQNCKDLFDLILTCEERVYDQVVEDLNSREQETCQPVHVVNVDIQDNHEEATLGAFLICELCQCIQHTEDMENEIDELLQEFEEKSGRTFLHTVCFY

Target ORF information:

RefSeq Version	NM_014188.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SSU72 homolog, RNA polymerase II CTD phosphatase (SSU72), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014188.3

ORF Insert Sequence:

ATGCCGTCGT CCCCGCTGCG GGTGGCGGTG GTGTGCTCGA GCAACCAGAA CCGGAGCATG 
GAGGCGCACA ACATCCTCAG CAAACGGGGA TTCAGCGTCC GATCCTTTGG AACAGGGACT 
CACGTGAAGC TTCCAGGACC AGCTCCCGAC AAGCCCAATG TTTATGATTT CAAAACCACA 
TATGACCAGA TGTACAATGA TCTTCTTAGG AAAGACAAAG AACTCTATAC ACAGAATGGG 
ATTTTACATA TGCTGGACAG AAATAAGAGA ATCAAGCCCC GGCCAGAAAG ATTCCAGAAC 
TGCAAAGACC TGTTTGATCT GATCCTCACT TGCGAAGAGA GAGTGTATGA CCAGGTGGTG 
GAAGATCTGA ATTCCAGAGA ACAGGAGACC TGCCAGCCTG TGCACGTGGT CAATGTGGAC 
ATCCAGGACA ACCACGAGGA GGCCACCCTG GGGGCGTTTC TCATCTGTGA GCTCTGCCAG 
TGTATCCAGC ACACGGAAGA CATGGAGAAC GAGATCGACG AGCTGCTGCA GGAGTTCGAG 
GAGAAGAGTG GCCGCACCTT TCTGCACACC GTCTGCTTCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural dissection of an interaction between transcription initiation and termination factors implicated in promoter-terminator crosstalk.
The Journal of biological chemistry(2017 Nov)
Bratkowski M,Unarta IC,Zhu L,Shubbar M,Huang X,Liu X

Functional interplay between Aurora B kinase and Ssu72 phosphatase regulates sister chromatid cohesion.
Nature communications42631(2013)
Kim HS,Kim SH,Park HY,Lee J,Yoon JH,Choi S,Ryu SH,Lee H,Cho HS,Lee CW

An unexpected binding mode for a Pol II CTD peptide phosphorylated at Ser7 in the active site of the CTD phosphatase Ssu72.
Genes & development26(20)2265-70(2012 Oct)
Xiang K,Manley JL,Tong L

Ssu72 phosphatase-dependent erasure of phospho-Ser7 marks on the RNA polymerase II C-terminal domain is essential for viability and transcription termination.
The Journal of biological chemistry287(11)8541-51(2012 Mar)
Zhang DW,Mosley AL,Ramisetty SR,Rodr?guez-Molina JB,Washburn MP,Ansari AZ

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SSU72 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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