UBE2T cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE2T
Entrez Gene ID	29089
Full Name	ubiquitin conjugating enzyme E2 T
Synonyms	FANCT,HSPC150,PIG50
General protein information	Preferred Names ubiquitin conjugating enzyme E2 T Names ubiquitin-conjugating enzyme E2 T E2 ubiquitin-conjugating enzyme T HSPC150 protein similar to ubiquitin-conjugating enzyme cell proliferation-inducing gene 50 protein ubiquitin carrier protein T ubiquitin conjugating enzyme E2T ubiquitin-conjugating enzyme E2T (putative) ubiquitin-protein ligase T
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q32.1
Summary	The protein encoded by this gene catalyzes the covalent attachment of ubiquitin to protein substrates. Defects in this gene have been associated with Fanconi anemia of complementation group T. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015].
Disorder MIM:	610538
Disorder Html:	Fanconi anemia, complementation group T, 616435 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_014176.3	NP_054895.1	ubiquitin-conjugating enzyme E2 T isoform 1
NM_014176.4	NP_054895.1	ubiquitin-conjugating enzyme E2 T isoform 1
NM_001310326.1	NP_001297255.1	ubiquitin-conjugating enzyme E2 T isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE2T	XP_514102.1
	UBE2T	XP_002808302.1
Rattus norvegicus (Norway rat)	Ube2t	NP_001101814.2
Gallus gallus (chicken)	UBE2T	XP_419230.2
Danio rerio (zebrafish)	ube2t	NP_001070763.1
Xenopus tropicalis (tropical clawed frog)	ube2t	XP_004910713.1
Homo sapiens (human)	UBE2T	NP_054895.1
Bos taurus (cattle)	UBE2T	NP_001033123.1
Mus musculus (house mouse)	Ube2t	NP_001265044.1
Oryza sativa (rice)	Os01g0605300	NP_001043518.1
Canis lupus familiaris (dog)	UBE2T	XP_848844.1

Gene Ontology
Bibliography

Related articles in PubMed

UBE2T promotes hepatocellular carcinoma cell growth via ubiquitination of p53.
Liu LP, Yang M, Peng QZ, Li MY, Zhang YS, Guo YH, Chen Y, Bao SY
Biochemical and biophysical research communications493(1)20-27(2017 11)

UBE2T knockdown inhibits gastric cancer progression.
Luo C, Yao Y, Yu Z, Zhou H, Guo L, Zhang J, Cao H, Zhang G, Li Y, Jiao Z
Oncotarget8(20)32639-32654(2017 May)

Allosteric Targeting of the Fanconi Anemia Ubiquitin-Conjugating Enzyme Ube2T by Fragment Screening.
Morreale FE, Bortoluzzi A, Chaugule VK, Arkinson C, Walden H, Ciulli A
Journal of medicinal chemistry60(9)4093-4098(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Collectively, our findings suggest UBE2T serves as a promising prognostic factor for HCC and functions as an oncogene. The newly identified miR-543/UBE2T/p53 axis may represent a new potential therapeutic target for HCC intervention.
Title: UBE2T promotes hepatocellular carcinoma cell growth via ubiquitination of p53.

report the identification of a new allosteric pocket on Ube2T through a fragment screening using biophysical methods. Several fragments binding to this site inhibit ubiquitin conjugation in vitro
Title: Allosteric Targeting of the Fanconi Anemia Ubiquitin-Conjugating Enzyme Ube2T by Fragment Screening.

UBE2T plays an important role in the tumorigenesis of gastric cancer and could act as a potential independent prognostic factor for cancer therapy.
Title: Ubiquitin-Conjugating Enzyme E2T is an Independent Prognostic Factor and Promotes Gastric Cancer Progression.

High UBE2T expression is associated with nasopharyngeal carcinoma.
Title: UBE2T promotes nasopharyngeal carcinoma cell proliferation, invasion, and metastasis by activating the AKT/GSK3?/?-catenin pathway.

Results showed that UBE2T was overexpressed in osteosarcoma tissues and cell lines. Moreover, UBE2T knockdown inhibited osteosarcoma cell proliferation, migration, and invasion.
Title: Knockdown of UBE2T Inhibits Osteosarcoma Cell Proliferation, Migration, and Invasion by Suppressing the PI3K/Akt Signaling Pathway.

The following UBE2T gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2T cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26222	NM_014176.4 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 T (UBE2T), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu67329	NM_001310326.1 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 T (UBE2T), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu26222	NM_014176.3	Homo sapiens ubiquitin conjugating enzyme E2 T (UBE2T), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE2T ( NM_014176.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_054895.1 Homo sapiens ubiquitin conjugating enzyme E2 T (UBE2T), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26222
Clone ID Related Accession (Same CDS sequence)	NM_014176.3 , NM_014176.4
Accession Version	NM_014176.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 T isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG776416.1 and AK000504.1. This sequence is a reference standard in the RefSeqGene project. On Oct 25, 2008 this sequence version replaced NM_014176.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF161499.1, SRR1163655.224309.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267760 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGAG CTTCACGTCT GAAGAGAGAG CTGCACATGT TAGCCACAGA GCCACCCCCA 
GGCATCACAT GTTGGCAAGA TAAAGACCAA ATGGATGACC TGCGAGCTCA AATATTAGGT 
GGAGCCAACA CACCTTATGA GAAAGGTGTT TTTAAGCTAG AAGTTATCAT TCCTGAGAGG 
TACCCATTTG AACCTCCTCA GATCCGATTT CTCACTCCAA TTTATCATCC AAACATTGAT 
TCTGCTGGAA GGATTTGTCT GGATGTTCTC AAATTGCCAC CAAAAGGTGC TTGGAGACCA 
TCCCTCAACA TCGCAACTGT GTTGACCTCT ATTCAGCTGC TCATGTCAGA ACCCAACCCT 
GATGACCCGC TCATGGCTGA CATATCCTCA GAATTTAAAT ATAATAAGCC AGCCTTCCTC 
AAGAATGCCA GACAGTGGAC AGAGAAGCAT GCAAGACAGA AACAAAAGGC TGATGAGGAA 
GAGATGCTTG ATAATCTACC AGAGGCTGGT GACTCCAGAG TACACAACTC AACACAGAAA 
AGGAAGGCCA GTCAGCTAGT AGGCATAGAA AAGAAATTTC ATCCTGATGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054895.1
CDS	137..730
Translation	MQRASRLKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQKADEEEMLDNLPEAGDSRVHNSTQKRKASQLVGIEKKFHPDV

Target ORF information:

RefSeq Version	NM_014176.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 T (UBE2T), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014176.3

ORF Insert Sequence:

ATGCAGAGAG CTTCACGTCT GAAGAGAGAG CTGCACATGT TAGCCACAGA GCCACCCCCA 
GGCATCACAT GTTGGCAAGA TAAAGACCAA ATGGATGACC TGCGAGCTCA AATATTAGGT 
GGAGCCAACA CACCTTATGA GAAAGGTGTT TTTAAGCTAG AAGTTATCAT TCCTGAGAGG 
TACCCATTTG AACCTCCTCA GATCCGATTT CTCACTCCAA TTTATCATCC AAACATTGAT 
TCTGCTGGAA GGATTTGTCT GGATGTTCTC AAATTGCCAC CAAAAGGTGC TTGGAGACCA 
TCCCTCAACA TCGCAACTGT GTTGACCTCT ATTCAGCTGC TCATGTCAGA ACCCAACCCT 
GATGACCCGC TCATGGCTGA CATATCCTCA GAATTTAAAT ATAATAAGCC AGCCTTCCTC 
AAGAATGCCA GACAGTGGAC AGAGAAGCAT GCAAGACAGA AACAAAAGGC TGATGAGGAA 
GAGATGCTTG ATAATCTACC AGAGGCTGGT GACTCCAGAG TACACAACTC AACACAGAAA 
AGGAAGGCCA GTCAGCTAGT AGGCATAGAA AAGAAATTTC ATCCTGATGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2T ( NM_014176.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_054895.1 Homo sapiens ubiquitin conjugating enzyme E2 T (UBE2T), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26222
Clone ID Related Accession (Same CDS sequence)	NM_014176.3 , NM_014176.4
Accession Version	NM_014176.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 T isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG776416.1 and AK000504.1. This sequence is a reference standard in the RefSeqGene project. On May 11, 2018 this sequence version replaced NM_014176.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF161499.1, SRR5189652.22045.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267760 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000646651.1/ ENSP00000494957.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGAG CTTCACGTCT GAAGAGAGAG CTGCACATGT TAGCCACAGA GCCACCCCCA 
GGCATCACAT GTTGGCAAGA TAAAGACCAA ATGGATGACC TGCGAGCTCA AATATTAGGT 
GGAGCCAACA CACCTTATGA GAAAGGTGTT TTTAAGCTAG AAGTTATCAT TCCTGAGAGG 
TACCCATTTG AACCTCCTCA GATCCGATTT CTCACTCCAA TTTATCATCC AAACATTGAT 
TCTGCTGGAA GGATTTGTCT GGATGTTCTC AAATTGCCAC CAAAAGGTGC TTGGAGACCA 
TCCCTCAACA TCGCAACTGT GTTGACCTCT ATTCAGCTGC TCATGTCAGA ACCCAACCCT 
GATGACCCGC TCATGGCTGA CATATCCTCA GAATTTAAAT ATAATAAGCC AGCCTTCCTC 
AAGAATGCCA GACAGTGGAC AGAGAAGCAT GCAAGACAGA AACAAAAGGC TGATGAGGAA 
GAGATGCTTG ATAATCTACC AGAGGCTGGT GACTCCAGAG TACACAACTC AACACAGAAA 
AGGAAGGCCA GTCAGCTAGT AGGCATAGAA AAGAAATTTC ATCCTGATGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054895.1
CDS	107..700
Translation	MQRASRLKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQKADEEEMLDNLPEAGDSRVHNSTQKRKASQLVGIEKKFHPDV

Target ORF information:

RefSeq Version	NM_014176.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 T (UBE2T), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014176.4

ORF Insert Sequence:

ATGCAGAGAG CTTCACGTCT GAAGAGAGAG CTGCACATGT TAGCCACAGA GCCACCCCCA 
GGCATCACAT GTTGGCAAGA TAAAGACCAA ATGGATGACC TGCGAGCTCA AATATTAGGT 
GGAGCCAACA CACCTTATGA GAAAGGTGTT TTTAAGCTAG AAGTTATCAT TCCTGAGAGG 
TACCCATTTG AACCTCCTCA GATCCGATTT CTCACTCCAA TTTATCATCC AAACATTGAT 
TCTGCTGGAA GGATTTGTCT GGATGTTCTC AAATTGCCAC CAAAAGGTGC TTGGAGACCA 
TCCCTCAACA TCGCAACTGT GTTGACCTCT ATTCAGCTGC TCATGTCAGA ACCCAACCCT 
GATGACCCGC TCATGGCTGA CATATCCTCA GAATTTAAAT ATAATAAGCC AGCCTTCCTC 
AAGAATGCCA GACAGTGGAC AGAGAAGCAT GCAAGACAGA AACAAAAGGC TGATGAGGAA 
GAGATGCTTG ATAATCTACC AGAGGCTGGT GACTCCAGAG TACACAACTC AACACAGAAA 
AGGAAGGCCA GTCAGCTAGT AGGCATAGAA AAGAAATTTC ATCCTGATGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2T ( NM_001310326.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001297255.1 Homo sapiens ubiquitin conjugating enzyme E2 T (UBE2T), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu67329
Clone ID Related Accession (Same CDS sequence)	NM_001310326.1
Accession Version	NM_001310326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 T isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP244265.1, BM460257.1 and AF161499.1. Transcript Variant: This variant (2) uses an alternate splice junction in a 5' exon and initiates translation at an alternate start codon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM460257.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119, SAMN03267751 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCTGCG AGCTCGTAGA AATATTAGGT GGAGCCAACA CACCTTATGA GAAAGGTGTT 
TTTAAGCTAG AAGTTATCAT TCCTGAGAGG TACCCATTTG AACCTCCTCA GATCCGATTT 
CTCACTCCAA TTTATCATCC AAACATTGAT TCTGCTGGAA GGATTTGTCT GGATGTTCTC 
AAATTGCCAC CAAAAGGTGC TTGGAGACCA TCCCTCAACA TCGCAACTGT GTTGACCTCT 
ATTCAGCTGC TCATGTCAGA ACCCAACCCT GATGACCCGC TCATGGCTGA CATATCCTCA 
GAATTTAAAT ATAATAAGCC AGCCTTCCTC AAGAATGCCA GACAGTGGAC AGAGAAGCAT 
GCAAGACAGA AACAAAAGGC TGATGAGGAA GAGATGCTTG ATAATCTACC AGAGGCTGGT 
GACTCCAGAG TACACAACTC AACACAGAAA AGGAAGGCCA GTCAGCTAGT AGGCATAGAA 
AAGAAATTTC ATCCTGATGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297255.1
CDS	231..734
Translation	MTCELVEILGGANTPYEKGVFKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQKADEEEMLDNLPEAGDSRVHNSTQKRKASQLVGIEKKFHPDV

Target ORF information:

RefSeq Version	NM_001310326.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 T (UBE2T), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310326.1

ORF Insert Sequence:

ATGACCTGCG AGCTCGTAGA AATATTAGGT GGAGCCAACA CACCTTATGA GAAAGGTGTT 
TTTAAGCTAG AAGTTATCAT TCCTGAGAGG TACCCATTTG AACCTCCTCA GATCCGATTT 
CTCACTCCAA TTTATCATCC AAACATTGAT TCTGCTGGAA GGATTTGTCT GGATGTTCTC 
AAATTGCCAC CAAAAGGTGC TTGGAGACCA TCCCTCAACA TCGCAACTGT GTTGACCTCT 
ATTCAGCTGC TCATGTCAGA ACCCAACCCT GATGACCCGC TCATGGCTGA CATATCCTCA 
GAATTTAAAT ATAATAAGCC AGCCTTCCTC AAGAATGCCA GACAGTGGAC AGAGAAGCAT 
GCAAGACAGA AACAAAAGGC TGATGAGGAA GAGATGCTTG ATAATCTACC AGAGGCTGGT 
GACTCCAGAG TACACAACTC AACACAGAAA AGGAAGGCCA GTCAGCTAGT AGGCATAGAA 
AAGAAATTTC ATCCTGATGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

UBE2T promotes hepatocellular carcinoma cell growth via ubiquitination of p53.
Biochemical and biophysical research communications493(1)20-27(2017 11)
Liu LP,Yang M,Peng QZ,Li MY,Zhang YS,Guo YH,Chen Y,Bao SY

UBE2T knockdown inhibits gastric cancer progression.
Oncotarget8(20)32639-32654(2017 May)
Luo C,Yao Y,Yu Z,Zhou H,Guo L,Zhang J,Cao H,Zhang G,Li Y,Jiao Z

Allosteric Targeting of the Fanconi Anemia Ubiquitin-Conjugating Enzyme Ube2T by Fragment Screening.
Journal of medicinal chemistry60(9)4093-4098(2017 May)
Morreale FE,Bortoluzzi A,Chaugule VK,Arkinson C,Walden H,Ciulli A

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UBE2T cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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