COA3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COA3
Entrez Gene ID	28958
Full Name	cytochrome c oxidase assembly factor 3
Synonyms	CCDC56,COX25,HSPC009,MITRAC12
General protein information	Preferred Names cytochrome c oxidase assembly factor 3 Names cytochrome c oxidase assembly factor 3 homolog, mitochondrial coiled-coil domain-containing protein 56 cytochrome C oxidase assembly factor 3 homolog, mitochondrial cytochrome c oxidase assembly protein 3 homolog, mitochondrial mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 12 kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.2
Summary	This gene encodes a member of the cytochrome c oxidase assembly factor family. Studies of a related gene in fly suggest that the encoded protein is localized to mitochondria and is essential for cytochrome c oxidase function. [provided by RefSeq, Nov 2012].
Disorder MIM:	614775

mRNA and Protein(s)

mRNA	Protein	Name
NM_001040431.2	NP_001035521.1	cytochrome c oxidase assembly factor 3 homolog, mitochondrial
NM_001040431.3	NP_001035521.1	cytochrome c oxidase assembly factor 3 homolog, mitochondrial

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Coa3	NP_001102517.1
Danio rerio (zebrafish)	coa3	NP_001108388.2
Canis lupus familiaris (dog)	CCDC56	NP_001239298.1
	LOC695277	XP_001083342.1
Bos taurus (cattle)	COA3	NP_001029855.1
Mus musculus (house mouse)	Coa3	NP_080894.1
Xenopus tropicalis (tropical clawed frog)	ccdc56	NP_001165768.1
Homo sapiens (human)	COA3	NP_001035521.1
Pan troglodytes (chimpanzee)	CCDC56	XP_001160561.1

Gene Ontology
Bibliography

Related articles in PubMed

Human COA3 Is an Oligomeric Highly Flexible Protein in Solution.
Neira JL, Mart?nez-Rodr?guez S, Hern?ndez-Cifre JG, C?mara-Artigas A, Clemente P, Peralta S, Fern?ndez-Moreno M?, Garesse R, Garc?a de la Torre J, Rizzuti B
Biochemistry55(45)6209-6220(2016 Nov)

COX assembly factor ccdc56 regulates mitochondrial morphology by affecting mitochondrial recruitment of Drp1.
Ban-Ishihara R, Tomohiro-Takamiya S, Tani M, Baudier J, Ishihara N, Kuge O
FEBS letters589(20 Pt B)3126-32(2015 Oct)

MITRAC7 Acts as a COX1-Specific Chaperone and Reveals a Checkpoint during Cytochrome c Oxidase Assembly.
Dennerlein S, Oeljeklaus S, Jans D, Hellwig C, Bareth B, Jakobs S, Deckers M, Warscheid B, Rehling P
Cell reports12(10)1644-55(2015 Sep)

Mutations in COA3 cause isolated complex IV deficiency associated with neuropathy, exercise intolerance, obesity, and short stature.
Ostergaard E, Weraarpachai W, Ravn K, Born AP, J?nson L, Duno M, Wibrand F, Shoubridge EA, Vissing J
Journal of medical genetics52(3)203-7(2015 Mar)

hCOA3 stabilizes cytochrome c oxidase 1 (COX1) and promotes cytochrome c oxidase assembly in human mitochondria.
Clemente P, Peralta S, Cruz-Bermudez A, Echevarr?a L, Fontanesi F, Barrientos A, Fernandez-Moreno MA, Garesse R
The Journal of biological chemistry288(12)8321-31(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that COX assembly factor 3 (hCOA3) is a disordered protein and that the flexibility of hCOA3 is crucial for its interaction with other proteins to favor mitochondrial protein translocation and assembly of proteins involved in the respiratory chain.
Title: Human COA3 Is an Oligomeric Highly Flexible Protein in Solution.

Data show that mitochondrial protein ccdc56/MITRAC12/COA3 positively regulates mitochondrial fission by recruiting the fission factor Drp1.
Title: COX assembly factor ccdc56 regulates mitochondrial morphology by affecting mitochondrial recruitment of Drp1.

COX14 was undetectable in COA3 subject fibroblasts, and that COA3 was undetectable in fibroblasts from a COX14 subject, demonstrating the interdependence of these two COX assembly factors
Title: Mutations in COA3 cause isolated complex IV deficiency associated with neuropathy, exercise intolerance, obesity, and short stature.

hCOA3 stabilizes COX1 co-translationally and promotes its assembly with COX partner subunits.
Title: hCOA3 stabilizes cytochrome c oxidase 1 (COX1) and promotes cytochrome c oxidase assembly in human mitochondria.

Study comprehensively identified mammalian cytochrome c oxidase assembly factors. These analyses led to the discoveryof MITRAC12, which defines the MITRAC (mitochondrial translation regulation assembly intermediate of cytochrome c oxidase)complexes.
Title: MITRAC links mitochondrial protein translocation to respiratory-chain assembly and translational regulation.

The following COA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14809	NM_001040431.3 Latest version!	Homo sapiens cytochrome c oxidase assembly factor 3 (COA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu14809	NM_001040431.2	Homo sapiens cytochrome c oxidase assembly factor 3 (COA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COA3 ( NM_001040431.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035521.1 Homo sapiens cytochrome c oxidase assembly factor 3 (COA3), mRNA.

Price & Availability↑

CloneID	OHu14809
Clone ID Related Accession (Same CDS sequence)	NM_001040431.2 , NM_001040431.3
Accession Version	NM_001040431.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	cytochrome c oxidase assembly factor 3 homolog, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL524532.3, BC002698.2, AC016889.28 and AU122748.1. This sequence is a reference standard in the RefSeqGene project. On Nov 17, 2012 this sequence version replaced NM_001040431.1. ##Evidence-Data-START## Transcript exon combination :: AU122748.1, SRR1660805.46291.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMN01820695 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCTT CGGGAGCTGG TGACCCTCTG GATTCTAAGC GTGGAGAGGC CCCGTTCGCT 
CAGCGTATCG ACCCGACTCG GGAGAAGCTG ACACCCGAGC AACTGCATTC CATGCGGCAG 
GCGGAGCTTG CCCAGTGGCA GAAGGTCCTA CCACGGCGGC GAACCCGGAA CATCGTGACC 
GGCCTAGGCA TCGGGGCCCT GGTGTTGGCT ATTTATGGTT ACACCTTCTA CTCGATTTCC 
CAGGAGCGTT TCCTAGATGA GCTAGAAGAC GAGGCCAAAG CTGCCCGAGC CCGAGCTCTG 
GCAAGGGCGT CAGGGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035521.1
CDS	45..365
Translation	MASSGAGDPLDSKRGEAPFAQRIDPTREKLTPEQLHSMRQAELAQWQKVLPRRRTRNIVTGLGIGALVLAIYGYTFYSISQERFLDELEDEAKAARARALARASGS

Target ORF information:

RefSeq Version	NM_001040431.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome c oxidase assembly factor 3 (COA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040431.2

ORF Insert Sequence:

ATGGCGTCTT CGGGAGCTGG TGACCCTCTG GATTCTAAGC GTGGAGAGGC CCCGTTCGCT 
CAGCGTATCG ACCCGACTCG GGAGAAGCTG ACACCCGAGC AACTGCATTC CATGCGGCAG 
GCGGAGCTTG CCCAGTGGCA GAAGGTCCTA CCACGGCGGC GAACCCGGAA CATCGTGACC 
GGCCTAGGCA TCGGGGCCCT GGTGTTGGCT ATTTATGGTT ACACCTTCTA CTCGATTTCC 
CAGGAGCGTT TCCTAGATGA GCTAGAAGAC GAGGCCAAAG CTGCCCGAGC CCGAGCTCTG 
GCAAGGGCGT CAGGGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COA3 ( NM_001040431.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035521.1 Homo sapiens cytochrome c oxidase assembly factor 3 (COA3), mRNA.

Price & Availability↑

CloneID	OHu14809
Clone ID Related Accession (Same CDS sequence)	NM_001040431.2 , NM_001040431.3
Accession Version	NM_001040431.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	cytochrome c oxidase assembly factor 3 homolog, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL524532.3, BC002698.2 and AC016889.28. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001040431.2. ##Evidence-Data-START## Transcript exon combination :: AK225611.1, SRR5189661.151737.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMN01820695 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000328434.8/ ENSP00000354762.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGTCTT CGGGAGCTGG TGACCCTCTG GATTCTAAGC GTGGAGAGGC CCCGTTCGCT 
CAGCGTATCG ACCCGACTCG GGAGAAGCTG ACACCCGAGC AACTGCATTC CATGCGGCAG 
GCGGAGCTTG CCCAGTGGCA GAAGGTCCTA CCACGGCGGC GAACCCGGAA CATCGTGACC 
GGCCTAGGCA TCGGGGCCCT GGTGTTGGCT ATTTATGGTT ACACCTTCTA CTCGATTTCC 
CAGGAGCGTT TCCTAGATGA GCTAGAAGAC GAGGCCAAAG CTGCCCGAGC CCGAGCTCTG 
GCAAGGGCGT CAGGGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035521.1
CDS	24..344
Translation	MASSGAGDPLDSKRGEAPFAQRIDPTREKLTPEQLHSMRQAELAQWQKVLPRRRTRNIVTGLGIGALVLAIYGYTFYSISQERFLDELEDEAKAARARALARASGS

Target ORF information:

RefSeq Version	NM_001040431.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome c oxidase assembly factor 3 (COA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040431.3

ORF Insert Sequence:

ATGGCGTCTT CGGGAGCTGG TGACCCTCTG GATTCTAAGC GTGGAGAGGC CCCGTTCGCT 
CAGCGTATCG ACCCGACTCG GGAGAAGCTG ACACCCGAGC AACTGCATTC CATGCGGCAG 
GCGGAGCTTG CCCAGTGGCA GAAGGTCCTA CCACGGCGGC GAACCCGGAA CATCGTGACC 
GGCCTAGGCA TCGGGGCCCT GGTGTTGGCT ATTTATGGTT ACACCTTCTA CTCGATTTCC 
CAGGAGCGTT TCCTAGATGA GCTAGAAGAC GAGGCCAAAG CTGCCCGAGC CCGAGCTCTG 
GCAAGGGCGT CAGGGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human COA3 Is an Oligomeric Highly Flexible Protein in Solution.
Biochemistry55(45)6209-6220(2016 Nov)
Neira JL,Mart?nez-Rodr?guez S,Hern?ndez-Cifre JG,C?mara-Artigas A,Clemente P,Peralta S,Fern?ndez-Moreno M?,Garesse R,Garc?a de la Torre J,Rizzuti B

COX assembly factor ccdc56 regulates mitochondrial morphology by affecting mitochondrial recruitment of Drp1.
FEBS letters589(20 Pt B)3126-32(2015 Oct)
Ban-Ishihara R,Tomohiro-Takamiya S,Tani M,Baudier J,Ishihara N,Kuge O

MITRAC7 Acts as a COX1-Specific Chaperone and Reveals a Checkpoint during Cytochrome c Oxidase Assembly.
Cell reports12(10)1644-55(2015 Sep)
Dennerlein S,Oeljeklaus S,Jans D,Hellwig C,Bareth B,Jakobs S,Deckers M,Warscheid B,Rehling P

Mutations in COA3 cause isolated complex IV deficiency associated with neuropathy, exercise intolerance, obesity, and short stature.
Journal of medical genetics52(3)203-7(2015 Mar)
Ostergaard E,Weraarpachai W,Ravn K,Born AP,J?nson L,Duno M,Wibrand F,Shoubridge EA,Vissing J

hCOA3 stabilizes cytochrome c oxidase 1 (COX1) and promotes cytochrome c oxidase assembly in human mitochondria.
The Journal of biological chemistry288(12)8321-31(2013 Mar)
Clemente P,Peralta S,Cruz-Bermudez A,Echevarr?a L,Fontanesi F,Barrientos A,Fernandez-Moreno MA,Garesse R

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COA3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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