H2BW2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	H2BW2
Entrez Gene ID	286436
Full Name	H2B histone family member M
General protein information	Preferred Names H2B histone family member M Names histone H2B type F-M H2B/s histone H2B.s
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq22.2
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the H2B histone family. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_011530922.1	XP_011529224.1	histone H2B type F-M isoform X1
XM_006724640.2	XP_006724703.1	histone H2B type F-M isoform X1
XM_006724640.1	XP_006724703.1	histone H2B type F-M isoform X1
NM_001164416.1	NP_001157888.1	histone H2B type F-M
XM_011530922.2	XP_011529224.1	histone H2B type F-M isoform X1
NM_001377073.1	NP_001364002.1	histone H2B type F-M

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	H2bfm	NP_081343.1
Homo sapiens (human)	H2BFM	NP_001157888.1
	LOC698930	XP_001087407.2

Gene Ontology
Bibliography

Related articles in PubMed

MS_HistoneDB, a manually curated resource for proteomic analysis of human and mouse histones.
El Kennani S, Adrait A, Shaytan AK, Khochbin S, Bruley C, Panchenko AR, Landsman D, Pflieger D, Govin J
Epigenetics & chromatin102(2017)

A unified phylogeny-based nomenclature for histone variants.
Talbert PB, Ahmad K, Almouzni G, Ausi? J, Berger F, Bhalla PL, Bonner WM, Cande WZ, Chadwick BP, Chan SW, Cross GA, Cui L, Dimitrov SI, Doenecke D, Eirin-L?pez JM, Gorovsky MA, Hake SB, Hamkalo BA, Holec S, Jacobsen SE, Kamieniarz K, Khochbin S, Ladurner AG, Landsman D, Latham JA, Loppin B, Malik HS, Marzluff WF, Pehrson JR, Postberg J, Schneider R, Singh MB, Smith MM, Thompson E, Torres-Padilla ME, Tremethick DJ, Turner BM, Waterborg JH, Wollmann H, Yelagandula R, Zhu B, Henikoff S
Epigenetics & chromatin57(2012 Jun)

The DNA sequence of the human X chromosome.
Ross MT, Grafham DV, Coffey AJ, Scherer S, McLay K, Muzny D, Platzer M, Howell GR, Burrows C, Bird CP, Frankish A, Lovell FL, Howe KL, Ashurst JL, Fulton RS, Sudbrak R, Wen G, Jones MC, Hurles ME, Andrews TD, Scott CE, Searle S, Ramser J, Whittaker A, Deadman R, Carter NP, Hunt SE, Chen R, Cree A, Gunaratne P, Havlak P, Hodgson A, Metzker ML, Richards S, Scott G, Steffen D, Sodergren E, Wheeler DA, Worley KC, Ainscough R, Ambrose KD, Ansari-Lari MA, Aradhya S, Ashwell RI, Babbage AK, Bagguley CL, Ballabio A, Banerjee R, Barker GE, Barlow KF, Barrett IP, Bates KN, Beare DM, Beasley H, Beasley O, Beck A, Bethel G, Blechschmidt K, Brady N, Bray-Allen S, Bridgeman AM, Brown AJ, Brown MJ, Bonnin D, Bruford EA, Buhay C, Burch P, Burford D, Burgess J, Burrill W, Burton J, Bye JM, Carder C, Carrel L, Chako J, Chapman JC, Chavez D, Chen E, Chen G, Chen Y, Chen Z, Chinault C, Ciccodicola A, Clark SY, Clarke G, Clee CM, Clegg S, Clerc-Blankenburg K, Clifford K, Cobley V, Cole CG, Conquer JS, Corby N, Connor RE, David R, Davies J, Davis C, Davis J, Delgado O, Deshazo D, Dhami P, Ding Y, Dinh H, Dodsworth S, Draper H, Dugan-Rocha S, Dunham A, Dunn M, Durbin KJ, Dutta I, Eades T, Ellwood M, Emery-Cohen A, Errington H, Evans KL, Faulkner L, Francis F, Frankland J, Fraser AE, Galgoczy P, Gilbert J, Gill R, Gl?ckner G, Gregory SG, Gribble S, Griffiths C, Grocock R, Gu Y, Gwilliam R, Hamilton C, Hart EA, Hawes A, Heath PD, Heitmann K, Hennig S, Hernandez J, Hinzmann B, Ho S, Hoffs M, Howden PJ, Huckle EJ, Hume J, Hunt PJ, Hunt AR, Isherwood J, Jacob L, Johnson D, Jones S, de Jong PJ, Joseph SS, Keenan S, Kelly S, Kershaw JK, Khan Z, Kioschis P, Klages S, Knights AJ, Kosiura A, Kovar-Smith C, Laird GK, Langford C, Lawlor S, Leversha M, Lewis L, Liu W, Lloyd C, Lloyd DM, Loulseged H, Loveland JE, Lovell JD, Lozado R, Lu J, Lyne R, Ma J, Maheshwari M, Matthews LH, McDowall J, McLaren S, McMurray A, Meidl P, Meitinger T, Milne S, Miner G, Mistry SL, Morgan M, Morris S, M?ller I, Mullikin JC, Nguyen N, Nordsiek G, Nyakatura G, O'Dell CN, Okwuonu G, Palmer S, Pandian R, Parker D, Parrish J, Pasternak S, Patel D, Pearce AV, Pearson DM, Pelan SE, Perez L, Porter KM, Ramsey Y, Reichwald K, Rhodes S, Ridler KA, Schlessinger D, Schueler MG, Sehra HK, Shaw-Smith C, Shen H, Sheridan EM, Shownkeen R, Skuce CD, Smith ML, Sotheran EC, Steingruber HE, Steward CA, Storey R, Swann RM, Swarbreck D, Tabor PE, Taudien S, Taylor T, Teague B, Thomas K, Thorpe A, Timms K, Tracey A, Trevanion S, Tromans AC, d'Urso M, Verduzco D, Villasana D, Waldron L, Wall M, Wang Q, Warren J, Warry GL, Wei X, West A, Whitehead SL, Whiteley MN, Wilkinson JE, Willey DL, Williams G, Williams L, Williamson A, Williamson H, Wilming L, Woodmansey RL, Wray PW, Yen J, Zhang J, Zhou J, Zoghbi H, Zorilla S, Buck D, Reinhardt R, Poustka A, Rosenthal A, Lehrach H, Meindl A, Minx PJ, Hillier LW, Willard HF, Wilson RK, Waterston RH, Rice CM, Vaudin M, Coulson A, Nelson DL, Weinstock G, Sulston JE, Durbin R, Hubbard T, Gibbs RA, Beck S, Rogers J, Bentley DR
Nature434(7031)325-37(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following H2BW2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H2BW2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01694	NM_001377073.1 Latest version!	Homo sapiens H2B.W histone 2 (H2BW2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu01694	NM_001164416.1 Latest version!	Homo sapiens H2B.W histone 2 (H2BW2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu01694	XM_006724640.2 Latest version!	Homo sapiens H2B histone family member M (H2BFM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu01694	XM_011530922.2 Latest version!	Homo sapiens H2B.W histone 2 (H2BW2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu01694	XM_006724640.1	Homo sapiens H2B histone family, member M (H2BFM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu01694	XM_011530922.1	Homo sapiens H2B histone family, member M (H2BFM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H2BW2 ( NM_001164416.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157888.1 Homo sapiens H2B.W histone 2 (H2BW2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01694
Clone ID Related Accession (Same CDS sequence)	XM_011530922.1 , XM_006724640.2 , XM_006724640.1 , NM_001164416.1 , XM_011530922.2 , NM_001377073.1
Accession Version	NM_001164416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-11
Organism	Homo sapiens(human)
Product	histone H2B type F-M
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK093522.1, AC234782.5 and AI018051.1. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK093522.1, DB041370.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## replication-independent histone :: PMID: 22650316 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCTG CTTCCGCCAT GGCTGAGGCT TCCTCTGAGA CAACCTCGGA GGAAGGCCAG 
AGCATCCAGG AGCCCAAAGA GGCCAACTCC ACGAAGGCCC AGAAGCAGAA GAGGCGAGGG 
TGCCGAGGCT CCCGCAGGCG CCACGCCAAC CGCCGTGGGG ACAGCTTCGG GGACAGCTTC 
ACCCCCTATT TCCCCCGGGT GCTGAAGCAG GTTCACCAGG GCCTCAGCCT TTCCCAGGAG 
GCCGTGAGTG TCATGGATTC TATGATCCAT GACATATTGG ACCGCATCGC CACCGAGGCT 
GGTCAGCTGG CCCATTACAC CAAGCGCGTG ACCATCACCT CCCGGGACAT CCAGATGGCC 
GTGCGACTGC TGCTGCCGGG GAAGATGGGC AAGCTCGCCG AGGCCCAGGG CACGAATGCC 
GCCCTCAGAA CTTCATTATG TGCGATATGG CAACAGAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157888.1
CDS	29..493
Translation	MAAASAMAEASSETTSEEGQSIQEPKEANSTKAQKQKRRGCRGSRRRHANRRGDSFGDSFTPYFPRVLKQVHQGLSLSQEAVSVMDSMIHDILDRIATEAGQLAHYTKRVTITSRDIQMAVRLLLPGKMGKLAEAQGTNAALRTSLCAIWQQRK

Target ORF information:

RefSeq Version	NM_001164416.1
Organism	Homo sapiens(human)
Definition	Homo sapiens H2B.W histone 2 (H2BW2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164416.1

ORF Insert Sequence:

ATGGCCGCTG CTTCCGCCAT GGCTGAGGCT TCCTCTGAGA CAACCTCGGA GGAAGGCCAG 
AGCATCCAGG AGCCCAAAGA GGCCAACTCC ACGAAGGCCC AGAAGCAGAA GAGGCGAGGG 
TGCCGAGGCT CCCGCAGGCG CCACGCCAAC CGCCGTGGGG ACAGCTTCGG GGACAGCTTC 
ACCCCCTATT TCCCCCGGGT GCTGAAGCAG GTTCACCAGG GCCTCAGCCT TTCCCAGGAG 
GCCGTGAGTG TCATGGATTC TATGATCCAT GACATATTGG ACCGCATCGC CACCGAGGCT 
GGTCAGCTGG CCCATTACAC CAAGCGCGTG ACCATCACCT CCCGGGACAT CCAGATGGCC 
GTGCGACTGC TGCTGCCGGG GAAGATGGGC AAGCTCGCCG AGGCCCAGGG CACGAATGCC 
GCCCTCAGAA CTTCATTATG TGCGATATGG CAACAGAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H2BW2 ( NM_001377073.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001364002.1 Homo sapiens H2B.W histone 2 (H2BW2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01694
Clone ID Related Accession (Same CDS sequence)	XM_011530922.1 , XM_006724640.2 , XM_006724640.1 , NM_001164416.1 , XM_011530922.2 , NM_001377073.1
Accession Version	NM_001377073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-11
Organism	Homo sapiens(human)
Product	histone H2B type F-M
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC234782.5. On Dec 12, 2019 this sequence version replaced XM_006724640.2. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein replication-independent histone :: PMID: 22650316 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCTG CTTCCGCCAT GGCTGAGGCT TCCTCTGAGA CAACCTCGGA GGAAGGCCAG 
AGCATCCAGG AGCCCAAAGA GGCCAACTCC ACGAAGGCCC AGAAGCAGAA GAGGCGAGGG 
TGCCGAGGCT CCCGCAGGCG CCACGCCAAC CGCCGTGGGG ACAGCTTCGG GGACAGCTTC 
ACCCCCTATT TCCCCCGGGT GCTGAAGCAG GTTCACCAGG GCCTCAGCCT TTCCCAGGAG 
GCCGTGAGTG TCATGGATTC TATGATCCAT GACATATTGG ACCGCATCGC CACCGAGGCT 
GGTCAGCTGG CCCATTACAC CAAGCGCGTG ACCATCACCT CCCGGGACAT CCAGATGGCC 
GTGCGACTGC TGCTGCCGGG GAAGATGGGC AAGCTCGCCG AGGCCCAGGG CACGAATGCC 
GCCCTCAGAA CTTCATTATG TGCGATATGG CAACAGAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001364002.1
CDS	22..486
Translation	MAAASAMAEASSETTSEEGQSIQEPKEANSTKAQKQKRRGCRGSRRRHANRRGDSFGDSFTPYFPRVLKQVHQGLSLSQEAVSVMDSMIHDILDRIATEAGQLAHYTKRVTITSRDIQMAVRLLLPGKMGKLAEAQGTNAALRTSLCAIWQQRK

Target ORF information:

RefSeq Version	NM_001377073.1
Organism	Homo sapiens(human)
Definition	Homo sapiens H2B.W histone 2 (H2BW2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377073.1

ORF Insert Sequence:

ATGGCCGCTG CTTCCGCCAT GGCTGAGGCT TCCTCTGAGA CAACCTCGGA GGAAGGCCAG 
AGCATCCAGG AGCCCAAAGA GGCCAACTCC ACGAAGGCCC AGAAGCAGAA GAGGCGAGGG 
TGCCGAGGCT CCCGCAGGCG CCACGCCAAC CGCCGTGGGG ACAGCTTCGG GGACAGCTTC 
ACCCCCTATT TCCCCCGGGT GCTGAAGCAG GTTCACCAGG GCCTCAGCCT TTCCCAGGAG 
GCCGTGAGTG TCATGGATTC TATGATCCAT GACATATTGG ACCGCATCGC CACCGAGGCT 
GGTCAGCTGG CCCATTACAC CAAGCGCGTG ACCATCACCT CCCGGGACAT CCAGATGGCC 
GTGCGACTGC TGCTGCCGGG GAAGATGGGC AAGCTCGCCG AGGCCCAGGG CACGAATGCC 
GCCCTCAGAA CTTCATTATG TGCGATATGG CAACAGAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H2BW2 ( XM_011530922.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529224.1 Homo sapiens H2B histone family, member M (H2BFM), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu01694
Clone ID Related Accession (Same CDS sequence)	XM_011530922.1 , XM_006724640.2 , XM_006724640.1 , NM_001164416.1 , XM_011530922.2 , NM_001377073.1
Accession Version	XM_011530922.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	histone H2B type F-M isoform X1
Comment

ATGGCCGCTG CTTCCGCCAT GGCTGAGGCT TCCTCTGAGA CAACCTCGGA GGAAGGCCAG 
AGCATCCAGG AGCCCAAAGA GGCCAACTCC ACGAAGGCCC AGAAGCAGAA GAGGCGAGGG 
TGCCGAGGCT CCCGCAGGCG CCACGCCAAC CGCCGTGGGG ACAGCTTCGG GGACAGCTTC 
ACCCCCTATT TCCCCCGGGT GCTGAAGCAG GTTCACCAGG GCCTCAGCCT TTCCCAGGAG 
GCCGTGAGTG TCATGGATTC TATGATCCAT GACATATTGG ACCGCATCGC CACCGAGGCT 
GGTCAGCTGG CCCATTACAC CAAGCGCGTG ACCATCACCT CCCGGGACAT CCAGATGGCC 
GTGCGACTGC TGCTGCCGGG GAAGATGGGC AAGCTCGCCG AGGCCCAGGG CACGAATGCC 
GCCCTCAGAA CTTCATTATG TGCGATATGG CAACAGAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529224.1
Translation	MAAASAMAEASSETTSEEGQSIQEPKEANSTKAQKQKRRGCRGSRRRHANRRGDSFGDSFTPYFPRVLKQVHQGLSLSQEAVSVMDSMIHDILDRIATEAGQLAHYTKRVTITSRDIQMAVRLLLPGKMGKLAEAQGTNAALRTSLCAIWQQRK

Target ORF information:

RefSeq Version	XM_011530922.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens H2B histone family, member M (H2BFM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530922.1

ORF Insert Sequence:

ATGGCCGCTG CTTCCGCCAT GGCTGAGGCT TCCTCTGAGA CAACCTCGGA GGAAGGCCAG 
AGCATCCAGG AGCCCAAAGA GGCCAACTCC ACGAAGGCCC AGAAGCAGAA GAGGCGAGGG 
TGCCGAGGCT CCCGCAGGCG CCACGCCAAC CGCCGTGGGG ACAGCTTCGG GGACAGCTTC 
ACCCCCTATT TCCCCCGGGT GCTGAAGCAG GTTCACCAGG GCCTCAGCCT TTCCCAGGAG 
GCCGTGAGTG TCATGGATTC TATGATCCAT GACATATTGG ACCGCATCGC CACCGAGGCT 
GGTCAGCTGG CCCATTACAC CAAGCGCGTG ACCATCACCT CCCGGGACAT CCAGATGGCC 
GTGCGACTGC TGCTGCCGGG GAAGATGGGC AAGCTCGCCG AGGCCCAGGG CACGAATGCC 
GCCCTCAGAA CTTCATTATG TGCGATATGG CAACAGAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H2BW2 ( XM_006724640.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724703.1 Homo sapiens H2B histone family member M (H2BFM), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu01694
Clone ID Related Accession (Same CDS sequence)	XM_011530922.1 , XM_006724640.2 , XM_006724640.1 , NM_001164416.1 , XM_011530922.2 , NM_001377073.1
Accession Version	XM_006724640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	histone H2B type F-M isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006724640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGCCGCTG CTTCCGCCAT GGCTGAGGCT TCCTCTGAGA CAACCTCGGA GGAAGGCCAG 
AGCATCCAGG AGCCCAAAGA GGCCAACTCC ACGAAGGCCC AGAAGCAGAA GAGGCGAGGG 
TGCCGAGGCT CCCGCAGGCG CCACGCCAAC CGCCGTGGGG ACAGCTTCGG GGACAGCTTC 
ACCCCCTATT TCCCCCGGGT GCTGAAGCAG GTTCACCAGG GCCTCAGCCT TTCCCAGGAG 
GCCGTGAGTG TCATGGATTC TATGATCCAT GACATATTGG ACCGCATCGC CACCGAGGCT 
GGTCAGCTGG CCCATTACAC CAAGCGCGTG ACCATCACCT CCCGGGACAT CCAGATGGCC 
GTGCGACTGC TGCTGCCGGG GAAGATGGGC AAGCTCGCCG AGGCCCAGGG CACGAATGCC 
GCCCTCAGAA CTTCATTATG TGCGATATGG CAACAGAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724703.1
CDS	62..526
Translation	MAAASAMAEASSETTSEEGQSIQEPKEANSTKAQKQKRRGCRGSRRRHANRRGDSFGDSFTPYFPRVLKQVHQGLSLSQEAVSVMDSMIHDILDRIATEAGQLAHYTKRVTITSRDIQMAVRLLLPGKMGKLAEAQGTNAALRTSLCAIWQQRK

Target ORF information:

RefSeq Version	XM_006724640.2
Organism	Homo sapiens(human)
Definition	Homo sapiens H2B histone family member M (H2BFM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724640.2

ORF Insert Sequence:

ATGGCCGCTG CTTCCGCCAT GGCTGAGGCT TCCTCTGAGA CAACCTCGGA GGAAGGCCAG 
AGCATCCAGG AGCCCAAAGA GGCCAACTCC ACGAAGGCCC AGAAGCAGAA GAGGCGAGGG 
TGCCGAGGCT CCCGCAGGCG CCACGCCAAC CGCCGTGGGG ACAGCTTCGG GGACAGCTTC 
ACCCCCTATT TCCCCCGGGT GCTGAAGCAG GTTCACCAGG GCCTCAGCCT TTCCCAGGAG 
GCCGTGAGTG TCATGGATTC TATGATCCAT GACATATTGG ACCGCATCGC CACCGAGGCT 
GGTCAGCTGG CCCATTACAC CAAGCGCGTG ACCATCACCT CCCGGGACAT CCAGATGGCC 
GTGCGACTGC TGCTGCCGGG GAAGATGGGC AAGCTCGCCG AGGCCCAGGG CACGAATGCC 
GCCCTCAGAA CTTCATTATG TGCGATATGG CAACAGAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H2BW2 ( XM_006724640.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724703.1 Homo sapiens H2B histone family, member M (H2BFM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu01694
Clone ID Related Accession (Same CDS sequence)	XM_011530922.1 , XM_006724640.2 , XM_006724640.1 , NM_001164416.1 , XM_011530922.2 , NM_001377073.1
Accession Version	XM_006724640.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	histone H2B type F-M isoform X1
Comment

ATGGCCGCTG CTTCCGCCAT GGCTGAGGCT TCCTCTGAGA CAACCTCGGA GGAAGGCCAG 
AGCATCCAGG AGCCCAAAGA GGCCAACTCC ACGAAGGCCC AGAAGCAGAA GAGGCGAGGG 
TGCCGAGGCT CCCGCAGGCG CCACGCCAAC CGCCGTGGGG ACAGCTTCGG GGACAGCTTC 
ACCCCCTATT TCCCCCGGGT GCTGAAGCAG GTTCACCAGG GCCTCAGCCT TTCCCAGGAG 
GCCGTGAGTG TCATGGATTC TATGATCCAT GACATATTGG ACCGCATCGC CACCGAGGCT 
GGTCAGCTGG CCCATTACAC CAAGCGCGTG ACCATCACCT CCCGGGACAT CCAGATGGCC 
GTGCGACTGC TGCTGCCGGG GAAGATGGGC AAGCTCGCCG AGGCCCAGGG CACGAATGCC 
GCCCTCAGAA CTTCATTATG TGCGATATGG CAACAGAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724703.1
Translation	MAAASAMAEASSETTSEEGQSIQEPKEANSTKAQKQKRRGCRGSRRRHANRRGDSFGDSFTPYFPRVLKQVHQGLSLSQEAVSVMDSMIHDILDRIATEAGQLAHYTKRVTITSRDIQMAVRLLLPGKMGKLAEAQGTNAALRTSLCAIWQQRK

Target ORF information:

RefSeq Version	XM_006724640.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens H2B histone family, member M (H2BFM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724640.1

ORF Insert Sequence:

ATGGCCGCTG CTTCCGCCAT GGCTGAGGCT TCCTCTGAGA CAACCTCGGA GGAAGGCCAG 
AGCATCCAGG AGCCCAAAGA GGCCAACTCC ACGAAGGCCC AGAAGCAGAA GAGGCGAGGG 
TGCCGAGGCT CCCGCAGGCG CCACGCCAAC CGCCGTGGGG ACAGCTTCGG GGACAGCTTC 
ACCCCCTATT TCCCCCGGGT GCTGAAGCAG GTTCACCAGG GCCTCAGCCT TTCCCAGGAG 
GCCGTGAGTG TCATGGATTC TATGATCCAT GACATATTGG ACCGCATCGC CACCGAGGCT 
GGTCAGCTGG CCCATTACAC CAAGCGCGTG ACCATCACCT CCCGGGACAT CCAGATGGCC 
GTGCGACTGC TGCTGCCGGG GAAGATGGGC AAGCTCGCCG AGGCCCAGGG CACGAATGCC 
GCCCTCAGAA CTTCATTATG TGCGATATGG CAACAGAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H2BW2 ( XM_011530922.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529224.1 Homo sapiens H2B.W histone 2 (H2BW2), transcript variant X1, mRNA.

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CloneID	OHu01694
Clone ID Related Accession (Same CDS sequence)	XM_011530922.1 , XM_006724640.2 , XM_006724640.1 , NM_001164416.1 , XM_011530922.2 , NM_001377073.1
Accession Version	XM_011530922.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	histone H2B type F-M isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCTG CTTCCGCCAT GGCTGAGGCT TCCTCTGAGA CAACCTCGGA GGAAGGCCAG 
AGCATCCAGG AGCCCAAAGA GGCCAACTCC ACGAAGGCCC AGAAGCAGAA GAGGCGAGGG 
TGCCGAGGCT CCCGCAGGCG CCACGCCAAC CGCCGTGGGG ACAGCTTCGG GGACAGCTTC 
ACCCCCTATT TCCCCCGGGT GCTGAAGCAG GTTCACCAGG GCCTCAGCCT TTCCCAGGAG 
GCCGTGAGTG TCATGGATTC TATGATCCAT GACATATTGG ACCGCATCGC CACCGAGGCT 
GGTCAGCTGG CCCATTACAC CAAGCGCGTG ACCATCACCT CCCGGGACAT CCAGATGGCC 
GTGCGACTGC TGCTGCCGGG GAAGATGGGC AAGCTCGCCG AGGCCCAGGG CACGAATGCC 
GCCCTCAGAA CTTCATTATG TGCGATATGG CAACAGAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529224.1
CDS	225..689
Translation	MAAASAMAEASSETTSEEGQSIQEPKEANSTKAQKQKRRGCRGSRRRHANRRGDSFGDSFTPYFPRVLKQVHQGLSLSQEAVSVMDSMIHDILDRIATEAGQLAHYTKRVTITSRDIQMAVRLLLPGKMGKLAEAQGTNAALRTSLCAIWQQRK

Target ORF information:

RefSeq Version	XM_011530922.2
Organism	Homo sapiens(human)
Definition	Homo sapiens H2B.W histone 2 (H2BW2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530922.2

ORF Insert Sequence:

ATGGCCGCTG CTTCCGCCAT GGCTGAGGCT TCCTCTGAGA CAACCTCGGA GGAAGGCCAG 
AGCATCCAGG AGCCCAAAGA GGCCAACTCC ACGAAGGCCC AGAAGCAGAA GAGGCGAGGG 
TGCCGAGGCT CCCGCAGGCG CCACGCCAAC CGCCGTGGGG ACAGCTTCGG GGACAGCTTC 
ACCCCCTATT TCCCCCGGGT GCTGAAGCAG GTTCACCAGG GCCTCAGCCT TTCCCAGGAG 
GCCGTGAGTG TCATGGATTC TATGATCCAT GACATATTGG ACCGCATCGC CACCGAGGCT 
GGTCAGCTGG CCCATTACAC CAAGCGCGTG ACCATCACCT CCCGGGACAT CCAGATGGCC 
GTGCGACTGC TGCTGCCGGG GAAGATGGGC AAGCTCGCCG AGGCCCAGGG CACGAATGCC 
GCCCTCAGAA CTTCATTATG TGCGATATGG CAACAGAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MS_HistoneDB, a manually curated resource for proteomic analysis of human and mouse histones.
Epigenetics & chromatin102(2017)
El Kennani S,Adrait A,Shaytan AK,Khochbin S,Bruley C,Panchenko AR,Landsman D,Pflieger D,Govin J

A unified phylogeny-based nomenclature for histone variants.
Epigenetics & chromatin57(2012 Jun)
Talbert PB,Ahmad K,Almouzni G,Ausi? J,Berger F,Bhalla PL,Bonner WM,Cande WZ,Chadwick BP,Chan SW,Cross GA,Cui L,Dimitrov SI,Doenecke D,Eirin-L?pez JM,Gorovsky MA,Hake SB,Hamkalo BA,Holec S,Jacobsen SE,Kamieniarz K,Khochbin S,Ladurner AG,Landsman D,Latham JA,Loppin B,Malik HS,Marzluff WF,Pehrson JR,Postberg J,Schneider R,Singh MB,Smith MM,Thompson E,Torres-Padilla ME,Tremethick DJ,Turner BM,Waterborg JH,Wollmann H,Yelagandula R,Zhu B,Henikoff S

The DNA sequence of the human X chromosome.
Nature434(7031)325-37(2005 Mar)
Ross MT,Grafham DV,Coffey AJ,Scherer S,McLay K,Muzny D,Platzer M,Howell GR,Burrows C,Bird CP,Frankish A,Lovell FL,Howe KL,Ashurst JL,Fulton RS,Sudbrak R,Wen G,Jones MC,Hurles ME,Andrews TD,Scott CE,Searle S,Ramser J,Whittaker A,Deadman R,Carter NP,Hunt SE,Chen R,Cree A,Gunaratne P,Havlak P,Hodgson A,Metzker ML,Richards S,Scott G,Steffen D,Sodergren E,Wheeler DA,Worley KC,Ainscough R,Ambrose KD,Ansari-Lari MA,Aradhya S,Ashwell RI,Babbage AK,Bagguley CL,Ballabio A,Banerjee R,Barker GE,Barlow KF,Barrett IP,Bates KN,Beare DM,Beasley H,Beasley O,Beck A,Bethel G,Blechschmidt K,Brady N,Bray-Allen S,Bridgeman AM,Brown AJ,Brown MJ,Bonnin D,Bruford EA,Buhay C,Burch P,Burford D,Burgess J,Burrill W,Burton J,Bye JM,Carder C,Carrel L,Chako J,Chapman JC,Chavez D,Chen E,Chen G,Chen Y,Chen Z,Chinault C,Ciccodicola A,Clark SY,Clarke G,Clee CM,Clegg S,Clerc-Blankenburg K,Clifford K,Cobley V,Cole CG,Conquer JS,Corby N,Connor RE,David R,Davies J,Davis C,Davis J,Delgado O,Deshazo D,Dhami P,Ding Y,Dinh H,Dodsworth S,Draper H,Dugan-Rocha S,Dunham A,Dunn M,Durbin KJ,Dutta I,Eades T,Ellwood M,Emery-Cohen A,Errington H,Evans KL,Faulkner L,Francis F,Frankland J,Fraser AE,Galgoczy P,Gilbert J,Gill R,Gl?ckner G,Gregory SG,Gribble S,Griffiths C,Grocock R,Gu Y,Gwilliam R,Hamilton C,Hart EA,Hawes A,Heath PD,Heitmann K,Hennig S,Hernandez J,Hinzmann B,Ho S,Hoffs M,Howden PJ,Huckle EJ,Hume J,Hunt PJ,Hunt AR,Isherwood J,Jacob L,Johnson D,Jones S,de Jong PJ,Joseph SS,Keenan S,Kelly S,Kershaw JK,Khan Z,Kioschis P,Klages S,Knights AJ,Kosiura A,Kovar-Smith C,Laird GK,Langford C,Lawlor S,Leversha M,Lewis L,Liu W,Lloyd C,Lloyd DM,Loulseged H,Loveland JE,Lovell JD,Lozado R,Lu J,Lyne R,Ma J,Maheshwari M,Matthews LH,McDowall J,McLaren S,McMurray A,Meidl P,Meitinger T,Milne S,Miner G,Mistry SL,Morgan M,Morris S,M?ller I,Mullikin JC,Nguyen N,Nordsiek G,Nyakatura G,O'Dell CN,Okwuonu G,Palmer S,Pandian R,Parker D,Parrish J,Pasternak S,Patel D,Pearce AV,Pearson DM,Pelan SE,Perez L,Porter KM,Ramsey Y,Reichwald K,Rhodes S,Ridler KA,Schlessinger D,Schueler MG,Sehra HK,Shaw-Smith C,Shen H,Sheridan EM,Shownkeen R,Skuce CD,Smith ML,Sotheran EC,Steingruber HE,Steward CA,Storey R,Swann RM,Swarbreck D,Tabor PE,Taudien S,Taylor T,Teague B,Thomas K,Thorpe A,Timms K,Tracey A,Trevanion S,Tromans AC,d'Urso M,Verduzco D,Villasana D,Waldron L,Wall M,Wang Q,Warren J,Warry GL,Wei X,West A,Whitehead SL,Whiteley MN,Wilkinson JE,Willey DL,Williams G,Williams L,Williamson A,Williamson H,Wilming L,Woodmansey RL,Wray PW,Yen J,Zhang J,Zhou J,Zoghbi H,Zorilla S,Buck D,Reinhardt R,Poustka A,Rosenthal A,Lehrach H,Meindl A,Minx PJ,Hillier LW,Willard HF,Wilson RK,Waterston RH,Rice CM,Vaudin M,Coulson A,Nelson DL,Weinstock G,Sulston JE,Durbin R,Hubbard T,Gibbs RA,Beck S,Rogers J,Bentley DR

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H2BW2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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