P4HA3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	P4HA3
Entrez Gene ID	283208
Full Name	prolyl 4-hydroxylase subunit alpha 3
General protein information	Preferred Names prolyl 4-hydroxylase subunit alpha 3 Names prolyl 4-hydroxylase subunit alpha-3 4-PH alpha-3 C-P4H alpha III collagen prolyl 4-hydroxylase alpha(III) procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III prolyl 4-hydroxylase, alpha polypeptide III
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.4
Summary	This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014].
Disorder MIM:	608987

mRNA and Protein(s)

mRNA	Protein	Name
NM_182904.5	NP_878907.1	prolyl 4-hydroxylase subunit alpha-3 isoform 1 precursor
NM_182904.4	NP_878907.1	prolyl 4-hydroxylase subunit alpha-3 isoform 1 precursor
NM_001288748.1	NP_001275677.1	prolyl 4-hydroxylase subunit alpha-3 isoform 2 precursor
NM_001288748.2	NP_001275677.1	prolyl 4-hydroxylase subunit alpha-3 isoform 2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	P4HA3	XP_001174896.1
Danio rerio (zebrafish)	p4ha3	XP_691737.4
Canis lupus familiaris (dog)	P4HA3	XP_851718.1
Homo sapiens (human)	P4HA3	NP_878907.1
Mus musculus (house mouse)	P4ha3	NP_796135.3
Gallus gallus (chicken)	P4HA3	XP_417248.4
Bos taurus (cattle)	P4HA3	NP_001001598.1
Rattus norvegicus (Norway rat)	P4ha3	NP_942070.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

The length of peptide substrates has a marked effect on hydroxylation by the hypoxia-inducible factor prolyl 4-hydroxylases.
Koivunen P, Hirsil? M, Kivirikko KI, Myllyharju J
The Journal of biological chemistry281(39)28712-20(2006 Sep)

Identification and characterization of a third human, rat, and mouse collagen prolyl 4-hydroxylase isoenzyme.
Kukkola L, Hieta R, Kivirikko KI, Myllyharju J
The Journal of biological chemistry278(48)47685-93(2003 Nov)

Cloning of a novel prolyl 4-hydroxylase subunit expressed in the fibrous cap of human atherosclerotic plaque.
Van Den Diepstraten C, Papay K, Bolender Z, Brown A, Pickering JG
Circulation108(5)508-11(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

HIF-P4H, HIF-1alpha and HIF-2alpha are effective oxygen sensors
Title: The length of peptide substrates has a marked effect on hydroxylation by the hypoxia-inducible factor prolyl 4-hydroxylases.

analysis of collagen prolyl 4-hydroxylase isoenzyme 3, its expression and catalytic properties
Title: Identification and characterization of a third human, rat, and mouse collagen prolyl 4-hydroxylase isoenzyme.

The following P4HA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P4HA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02312	NM_182904.5 Latest version!	Homo sapiens prolyl 4-hydroxylase subunit alpha 3 (P4HA3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
OHu06351	NM_001288748.2 Latest version!	Homo sapiens prolyl 4-hydroxylase subunit alpha 3 (P4HA3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu02312	NM_182904.4	Homo sapiens prolyl 4-hydroxylase subunit alpha 3 (P4HA3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
OHu06351	NM_001288748.1	Homo sapiens prolyl 4-hydroxylase subunit alpha 3 (P4HA3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P4HA3 ( NM_182904.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_878907.1 Homo sapiens prolyl 4-hydroxylase subunit alpha 3 (P4HA3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02312
Clone ID Related Accession (Same CDS sequence)	NM_182904.5 , NM_182904.4
Accession Version	NM_182904.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	prolyl 4-hydroxylase subunit alpha-3 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY004548.1 and AY358521.1. On Nov 22, 2018 this sequence version replaced NM_182904.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (1). ##Evidence-Data-START## Transcript exon combination :: AY358521.1, AY313448.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000331597.9/ ENSP00000332170.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGTCCTG GGGCGCGGCT GGCGGCGCTG CTGGCGGTGC TGGCGCTCGG GACAGGAGAC 
CCAGAAAGGG CTGCGGCTCG GGGCGACACG TTCTCGGCGC TGACCAGCGT GGCGCGCGCC 
CTGGCGCCCG AGCGCCGGCT GCTGGGGCTG CTGAGGCGGT ACCTGCGCGG GGAGGAGGCG 
CGGCTGCGGG ACCTGACTAG ATTCTACGAC AAGGTACTTT CTTTGCATGA GGATTCAACA 
ACCCCTGTGG CTAACCCTCT GCTTGCATTT ACTCTCATCA AACGCCTGCA GTCTGACTGG 
AGGAATGTGG TACATAGTCT GGAGGCCAGT GAGAACATCC GAGCTCTGAA GGATGGCTAT 
GAGAAGGTGG AGCAAGACCT TCCAGCCTTT GAGGACCTTG AGGGAGCAGC AAGGGCCCTG 
ATGCGGCTGC AGGACGTGTA CATGCTCAAT GTGAAAGGCC TGGCCCGAGG TGTCTTTCAG 
AGAGTCACTG GCTCTGCCAT CACTGACCTG TACAGCCCCA AACGGCTCTT TTCTCTCACA 
GGGGATGACT GCTTCCAAGT TGGCAAGGTG GCCTATGACA TGGGGGATTA TTACCATGCC 
ATTCCATGGC TGGAGGAGGC TGTCAGTCTC TTCCGAGGAT CTTACGGAGA GTGGAAGACA 
GAGGATGAGG CAAGTCTAGA AGATGCCTTG GATCACTTGG CCTTTGCTTA TTTCCGGGCA 
GGAAATGTTT CGTGTGCCCT CAGCCTCTCT CGGGAGTTTC TTCTCTACAG CCCAGATAAT 
AAGAGGATGG CCAGGAATGT CTTGAAATAT GAAAGGCTCT TGGCAGAGAG CCCCAACCAC 
GTGGTAGCTG AGGCTGTCAT CCAGAGGCCC AATATACCCC ACCTGCAGAC CAGAGACACC 
TACGAGGGGC TATGTCAGAC CCTGGGTTCC CAGCCCACTC TCTACCAGAT CCCTAGCCTC 
TACTGTTCCT ATGAGACCAA TTCCAACGCC TACCTGCTGC TCCAGCCCAT CCGGAAGGAG 
GTCATCCACC TGGAGCCCTA CATTGCTCTC TACCATGACT TCGTCAGTGA CTCAGAGGCT 
CAGAAAATTA GAGAACTTGC AGAACCATGG CTACAGAGGT CAGTGGTGGC ATCAGGGGAG 
AAGCAGTTAC AAGTGGAGTA CCGCATCAGC AAAAGTGCCT GGCTGAAGGA CACTGTTGAC 
CCAAAACTGG TGACCCTCAA CCACCGCATT GCTGCCCTCA CAGGCCTTGA TGTCCGGCCT 
CCCTATGCAG AGTATCTGCA GGTGGTGAAC TATGGCATCG GAGGACACTA TGAGCCTCAC 
TTTGACCATG CTACGTCACC AAGCAGCCCC CTCTACAGAA TGAAGTCAGG AAACCGAGTT 
GCAACATTTA TGATCTATCT GAGCTCGGTG GAAGCTGGAG GAGCCACAGC CTTCATCTAT 
GCCAACCTCA GCGTGCCTGT GGTTAGGAAT GCAGCACTGT TTTGGTGGAA CCTGCACAGG 
AGTGGTGAAG GGGACAGTGA CACACTTCAT GCTGGCTGTC CTGTCCTGGT GGGAGATAAG 
TGGGTGGCCA ACAAGTGGAT ACATGAGTAT GGACAGGAAT TCCGCAGACC CTGCAGCTCC 
AGCCCTGAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_878907.1
CDS	30..1664
Translation	MGPGARLAALLAVLALGTGDPERAAARGDTFSALTSVARALAPERRLLGLLRRYLRGEEARLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEASENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAITDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFRAGNVSCALSLSREFLLYSPDNKRMARNVLKYERLLAESPNHVVAEAVIQRPNIPHLQTRDTYEGLCQTLGSQPTLYQIPSLYCSYETNSNAYLLLQPIRKEVIHLEPYIALYHDFVSDSEAQKIRELAEPWLQRSVVASGEKQLQVEYRISKSAWLKDTVDPKLVTLNHRIAALTGLDVRPPYAEYLQVVNYGIGGHYEPHFDHATSPSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIYANLSVPVVRNAALFWWNLHRSGEGDSDTLHAGCPVLVGDKWVANKWIHEYGQEFRRPCSSSPED

Target ORF information:

RefSeq Version	NM_182904.5
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 4-hydroxylase subunit alpha 3 (P4HA3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182904.5

ORF Insert Sequence:

ATGGGTCCTG GGGCGCGGCT GGCGGCGCTG CTGGCGGTGC TGGCGCTCGG GACAGGAGAC 
CCAGAAAGGG CTGCGGCTCG GGGCGACACG TTCTCGGCGC TGACCAGCGT GGCGCGCGCC 
CTGGCGCCCG AGCGCCGGCT GCTGGGGCTG CTGAGGCGGT ACCTGCGCGG GGAGGAGGCG 
CGGCTGCGGG ACCTGACTAG ATTCTACGAC AAGGTACTTT CTTTGCATGA GGATTCAACA 
ACCCCTGTGG CTAACCCTCT GCTTGCATTT ACTCTCATCA AACGCCTGCA GTCTGACTGG 
AGGAATGTGG TACATAGTCT GGAGGCCAGT GAGAACATCC GAGCTCTGAA GGATGGCTAT 
GAGAAGGTGG AGCAAGACCT TCCAGCCTTT GAGGACCTTG AGGGAGCAGC AAGGGCCCTG 
ATGCGGCTGC AGGACGTGTA CATGCTCAAT GTGAAAGGCC TGGCCCGAGG TGTCTTTCAG 
AGAGTCACTG GCTCTGCCAT CACTGACCTG TACAGCCCCA AACGGCTCTT TTCTCTCACA 
GGGGATGACT GCTTCCAAGT TGGCAAGGTG GCCTATGACA TGGGGGATTA TTACCATGCC 
ATTCCATGGC TGGAGGAGGC TGTCAGTCTC TTCCGAGGAT CTTACGGAGA GTGGAAGACA 
GAGGATGAGG CAAGTCTAGA AGATGCCTTG GATCACTTGG CCTTTGCTTA TTTCCGGGCA 
GGAAATGTTT CGTGTGCCCT CAGCCTCTCT CGGGAGTTTC TTCTCTACAG CCCAGATAAT 
AAGAGGATGG CCAGGAATGT CTTGAAATAT GAAAGGCTCT TGGCAGAGAG CCCCAACCAC 
GTGGTAGCTG AGGCTGTCAT CCAGAGGCCC AATATACCCC ACCTGCAGAC CAGAGACACC 
TACGAGGGGC TATGTCAGAC CCTGGGTTCC CAGCCCACTC TCTACCAGAT CCCTAGCCTC 
TACTGTTCCT ATGAGACCAA TTCCAACGCC TACCTGCTGC TCCAGCCCAT CCGGAAGGAG 
GTCATCCACC TGGAGCCCTA CATTGCTCTC TACCATGACT TCGTCAGTGA CTCAGAGGCT 
CAGAAAATTA GAGAACTTGC AGAACCATGG CTACAGAGGT CAGTGGTGGC ATCAGGGGAG 
AAGCAGTTAC AAGTGGAGTA CCGCATCAGC AAAAGTGCCT GGCTGAAGGA CACTGTTGAC 
CCAAAACTGG TGACCCTCAA CCACCGCATT GCTGCCCTCA CAGGCCTTGA TGTCCGGCCT 
CCCTATGCAG AGTATCTGCA GGTGGTGAAC TATGGCATCG GAGGACACTA TGAGCCTCAC 
TTTGACCATG CTACGTCACC AAGCAGCCCC CTCTACAGAA TGAAGTCAGG AAACCGAGTT 
GCAACATTTA TGATCTATCT GAGCTCGGTG GAAGCTGGAG GAGCCACAGC CTTCATCTAT 
GCCAACCTCA GCGTGCCTGT GGTTAGGAAT GCAGCACTGT TTTGGTGGAA CCTGCACAGG 
AGTGGTGAAG GGGACAGTGA CACACTTCAT GCTGGCTGTC CTGTCCTGGT GGGAGATAAG 
TGGGTGGCCA ACAAGTGGAT ACATGAGTAT GGACAGGAAT TCCGCAGACC CTGCAGCTCC 
AGCCCTGAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P4HA3 ( NM_182904.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_878907.1 Homo sapiens prolyl 4-hydroxylase subunit alpha 3 (P4HA3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02312
Clone ID Related Accession (Same CDS sequence)	NM_182904.5 , NM_182904.4
Accession Version	NM_182904.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-28
Organism	Homo sapiens(human)
Product	prolyl 4-hydroxylase subunit alpha-3 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY004548.1, AY358521.1 and HY188166.1. On Jan 3, 2014 this sequence version replaced NM_182904.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (1). ##Evidence-Data-START## Transcript exon combination :: AY313448.1, AY358521.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCCTG GGGCGCGGCT GGCGGCGCTG CTGGCGGTGC TGGCGCTCGG GACAGGAGAC 
CCAGAAAGGG CTGCGGCTCG GGGCGACACG TTCTCGGCGC TGACCAGCGT GGCGCGCGCC 
CTGGCGCCCG AGCGCCGGCT GCTGGGGCTG CTGAGGCGGT ACCTGCGCGG GGAGGAGGCG 
CGGCTGCGGG ACCTGACTAG ATTCTACGAC AAGGTACTTT CTTTGCATGA GGATTCAACA 
ACCCCTGTGG CTAACCCTCT GCTTGCATTT ACTCTCATCA AACGCCTGCA GTCTGACTGG 
AGGAATGTGG TACATAGTCT GGAGGCCAGT GAGAACATCC GAGCTCTGAA GGATGGCTAT 
GAGAAGGTGG AGCAAGACCT TCCAGCCTTT GAGGACCTTG AGGGAGCAGC AAGGGCCCTG 
ATGCGGCTGC AGGACGTGTA CATGCTCAAT GTGAAAGGCC TGGCCCGAGG TGTCTTTCAG 
AGAGTCACTG GCTCTGCCAT CACTGACCTG TACAGCCCCA AACGGCTCTT TTCTCTCACA 
GGGGATGACT GCTTCCAAGT TGGCAAGGTG GCCTATGACA TGGGGGATTA TTACCATGCC 
ATTCCATGGC TGGAGGAGGC TGTCAGTCTC TTCCGAGGAT CTTACGGAGA GTGGAAGACA 
GAGGATGAGG CAAGTCTAGA AGATGCCTTG GATCACTTGG CCTTTGCTTA TTTCCGGGCA 
GGAAATGTTT CGTGTGCCCT CAGCCTCTCT CGGGAGTTTC TTCTCTACAG CCCAGATAAT 
AAGAGGATGG CCAGGAATGT CTTGAAATAT GAAAGGCTCT TGGCAGAGAG CCCCAACCAC 
GTGGTAGCTG AGGCTGTCAT CCAGAGGCCC AATATACCCC ACCTGCAGAC CAGAGACACC 
TACGAGGGGC TATGTCAGAC CCTGGGTTCC CAGCCCACTC TCTACCAGAT CCCTAGCCTC 
TACTGTTCCT ATGAGACCAA TTCCAACGCC TACCTGCTGC TCCAGCCCAT CCGGAAGGAG 
GTCATCCACC TGGAGCCCTA CATTGCTCTC TACCATGACT TCGTCAGTGA CTCAGAGGCT 
CAGAAAATTA GAGAACTTGC AGAACCATGG CTACAGAGGT CAGTGGTGGC ATCAGGGGAG 
AAGCAGTTAC AAGTGGAGTA CCGCATCAGC AAAAGTGCCT GGCTGAAGGA CACTGTTGAC 
CCAAAACTGG TGACCCTCAA CCACCGCATT GCTGCCCTCA CAGGCCTTGA TGTCCGGCCT 
CCCTATGCAG AGTATCTGCA GGTGGTGAAC TATGGCATCG GAGGACACTA TGAGCCTCAC 
TTTGACCATG CTACGTCACC AAGCAGCCCC CTCTACAGAA TGAAGTCAGG AAACCGAGTT 
GCAACATTTA TGATCTATCT GAGCTCGGTG GAAGCTGGAG GAGCCACAGC CTTCATCTAT 
GCCAACCTCA GCGTGCCTGT GGTTAGGAAT GCAGCACTGT TTTGGTGGAA CCTGCACAGG 
AGTGGTGAAG GGGACAGTGA CACACTTCAT GCTGGCTGTC CTGTCCTGGT GGGAGATAAG 
TGGGTGGCCA ACAAGTGGAT ACATGAGTAT GGACAGGAAT TCCGCAGACC CTGCAGCTCC 
AGCCCTGAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_878907.1
CDS	66..1700
Translation	MGPGARLAALLAVLALGTGDPERAAARGDTFSALTSVARALAPERRLLGLLRRYLRGEEARLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEASENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAITDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFRAGNVSCALSLSREFLLYSPDNKRMARNVLKYERLLAESPNHVVAEAVIQRPNIPHLQTRDTYEGLCQTLGSQPTLYQIPSLYCSYETNSNAYLLLQPIRKEVIHLEPYIALYHDFVSDSEAQKIRELAEPWLQRSVVASGEKQLQVEYRISKSAWLKDTVDPKLVTLNHRIAALTGLDVRPPYAEYLQVVNYGIGGHYEPHFDHATSPSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIYANLSVPVVRNAALFWWNLHRSGEGDSDTLHAGCPVLVGDKWVANKWIHEYGQEFRRPCSSSPED

Target ORF information:

RefSeq Version	NM_182904.4
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 4-hydroxylase subunit alpha 3 (P4HA3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182904.4

ORF Insert Sequence:

ATGGGTCCTG GGGCGCGGCT GGCGGCGCTG CTGGCGGTGC TGGCGCTCGG GACAGGAGAC 
CCAGAAAGGG CTGCGGCTCG GGGCGACACG TTCTCGGCGC TGACCAGCGT GGCGCGCGCC 
CTGGCGCCCG AGCGCCGGCT GCTGGGGCTG CTGAGGCGGT ACCTGCGCGG GGAGGAGGCG 
CGGCTGCGGG ACCTGACTAG ATTCTACGAC AAGGTACTTT CTTTGCATGA GGATTCAACA 
ACCCCTGTGG CTAACCCTCT GCTTGCATTT ACTCTCATCA AACGCCTGCA GTCTGACTGG 
AGGAATGTGG TACATAGTCT GGAGGCCAGT GAGAACATCC GAGCTCTGAA GGATGGCTAT 
GAGAAGGTGG AGCAAGACCT TCCAGCCTTT GAGGACCTTG AGGGAGCAGC AAGGGCCCTG 
ATGCGGCTGC AGGACGTGTA CATGCTCAAT GTGAAAGGCC TGGCCCGAGG TGTCTTTCAG 
AGAGTCACTG GCTCTGCCAT CACTGACCTG TACAGCCCCA AACGGCTCTT TTCTCTCACA 
GGGGATGACT GCTTCCAAGT TGGCAAGGTG GCCTATGACA TGGGGGATTA TTACCATGCC 
ATTCCATGGC TGGAGGAGGC TGTCAGTCTC TTCCGAGGAT CTTACGGAGA GTGGAAGACA 
GAGGATGAGG CAAGTCTAGA AGATGCCTTG GATCACTTGG CCTTTGCTTA TTTCCGGGCA 
GGAAATGTTT CGTGTGCCCT CAGCCTCTCT CGGGAGTTTC TTCTCTACAG CCCAGATAAT 
AAGAGGATGG CCAGGAATGT CTTGAAATAT GAAAGGCTCT TGGCAGAGAG CCCCAACCAC 
GTGGTAGCTG AGGCTGTCAT CCAGAGGCCC AATATACCCC ACCTGCAGAC CAGAGACACC 
TACGAGGGGC TATGTCAGAC CCTGGGTTCC CAGCCCACTC TCTACCAGAT CCCTAGCCTC 
TACTGTTCCT ATGAGACCAA TTCCAACGCC TACCTGCTGC TCCAGCCCAT CCGGAAGGAG 
GTCATCCACC TGGAGCCCTA CATTGCTCTC TACCATGACT TCGTCAGTGA CTCAGAGGCT 
CAGAAAATTA GAGAACTTGC AGAACCATGG CTACAGAGGT CAGTGGTGGC ATCAGGGGAG 
AAGCAGTTAC AAGTGGAGTA CCGCATCAGC AAAAGTGCCT GGCTGAAGGA CACTGTTGAC 
CCAAAACTGG TGACCCTCAA CCACCGCATT GCTGCCCTCA CAGGCCTTGA TGTCCGGCCT 
CCCTATGCAG AGTATCTGCA GGTGGTGAAC TATGGCATCG GAGGACACTA TGAGCCTCAC 
TTTGACCATG CTACGTCACC AAGCAGCCCC CTCTACAGAA TGAAGTCAGG AAACCGAGTT 
GCAACATTTA TGATCTATCT GAGCTCGGTG GAAGCTGGAG GAGCCACAGC CTTCATCTAT 
GCCAACCTCA GCGTGCCTGT GGTTAGGAAT GCAGCACTGT TTTGGTGGAA CCTGCACAGG 
AGTGGTGAAG GGGACAGTGA CACACTTCAT GCTGGCTGTC CTGTCCTGGT GGGAGATAAG 
TGGGTGGCCA ACAAGTGGAT ACATGAGTAT GGACAGGAAT TCCGCAGACC CTGCAGCTCC 
AGCCCTGAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P4HA3 ( NM_001288748.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001275677.1 Homo sapiens prolyl 4-hydroxylase subunit alpha 3 (P4HA3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu06351
Clone ID Related Accession (Same CDS sequence)	NM_001288748.1 , NM_001288748.2
Accession Version	NM_001288748.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	prolyl 4-hydroxylase subunit alpha-3 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY004548.1, AK300598.1 and HY188166.1. On Jan 3, 2014 this sequence version replaced XM_005273923.1. Transcript Variant: This variant (2) uses an alternate splice site in the central coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a longer and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK300598.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGGTCCTG GGGCGCGGCT GGCGGCGCTG CTGGCGGTGC TGGCGCTCGG GACAGGAGAC 
CCAGAAAGGG CTGCGGCTCG GGGCGACACG TTCTCGGCGC TGACCAGCGT GGCGCGCGCC 
CTGGCGCCCG AGCGCCGGCT GCTGGGGCTG CTGAGGCGGT ACCTGCGCGG GGAGGAGGCG 
CGGCTGCGGG ACCTGACTAG ATTCTACGAC AAGGTACTTT CTTTGCATGA GGATTCAACA 
ACCCCTGTGG CTAACCCTCT GCTTGCATTT ACTCTCATCA AACGCCTGCA GTCTGACTGG 
AGGAATGTGG TACATAGTCT GGAGGCCAGT GAGAACATCC GAGCTCTGAA GGATGGCTAT 
GAGAAGGTGG AGCAAGACCT TCCAGCCTTT GAGGACCTTG AGGGAGCAGC AAGGGCCCTG 
ATGCGGCTGC AGGACGTGTA CATGCTCAAT GTGAAAGGCC TGGCCCGAGG TGTCTTTCAG 
AGAGTCACTG GCTCTGCCAT CACTGACCTG TACAGCCCCA AACGGCTCTT TTCTCTCACA 
GGGGATGACT GCTTCCAAGT TGGCAAGGTG GCCTATGACA TGGGGGATTA TTACCATGCC 
ATTCCATGGC TGGAGGAGGC TGTCAGTCTC TTCCGAGGAT CTTACGGAGA GTGGAAGACA 
GAGGATGAGG CAAGTCTAGA AGATGCCTTG GATCACTTGG CCTTTGCTTA TTTCCGGGCA 
GGAAATGTTT CGTGTGCCCT CAGCCTCTCT CGGGAGTTTC TTCTCTACAG CCCAGATAAT 
AAGAGGATGG CCAGGAATGT CTTGAAATAT GAAAGGCTCT TGGCAGAGAG CCCCAACCAC 
GTGGTAGCTG AGGCTGTCAT CCAGAGGCCC AATATACCCC ACCTGCAGAC CAGAGACACC 
TACGAGGGGC TATGTCAGAC CCTGGGTTCC CAGCCCACTC TCTACCAGAT CCCTAGCCTC 
TACTGTTCCT ATGAGACCAA TTCCAACGCC TACCTGCTGC TCCAGCCCAT CCGGAAGGAG 
GTCATCCACC TGGAGCCCTA CATTGCTCTC TACCATGACT TCGTCAGTGA CTCAGAGGCT 
CAGAAAATTA GAGAACTTGC AGAACCATGG CTACAGAGGT CAGTGGTGGC ATCAGGGGAG 
AAGCAGTTAC AAGTGGAGTA CCGCATCAGC AAAAGTGCCT GGCTGAAGGA CACTGTTGAC 
CCAAAACTGG TGACCCTCAA CCACCGCATT GCTGCCCTCA CAGGCCTTGA TGTCCGGCCT 
CCCTATGCAG AGTATCTGCA GGTGGTGAAC TATGGCATCG GAGGACACTA TGAGCCTCAC 
TTTGACCATG CTACGTCACC AAGCAGCCCC CTCTACAGAA TGAAGTCAGG AAACCGAGTT 
GCAACATTTA TGATCTATCT GAGCTCGGTG GAAGCTGGAG GAGCCACAGC CTTCATCTAT 
GCCAACCTCA GCGTGCCTGT GGTTAGGCAC TGTTTTGGTG GAACCTGCAC AGGAGTGGTG 
AAGGGGACAG TGACACACTT CATGCTGGCT GTCCTGTCCT GGTGGGAGAT AAGTGGGTGG 
CCAACAAGTG GATACATGAG TATGGACAGG AATTCCGCAG ACCCTGCAGC TCCAGCCCTG 
AAGACTGAAC TGTTGGCAGA GAGAAGCTGG TGGAGTCCTG TGGCTTTCCA GAGAAGCCAG 
GAGCCAAAAG CTGGGGTAGG AGAGGAGAAA GCAGAGCAGC CTCCTGGAAG AAGGCCTTGT 
CAGCTTTGTC TGTGCCTCGC AAATCAGAGG CAAGGGAGAG GTTGTTACCA GGGGACACTG 
AGAATGTACA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001275677.1
CDS	66..1880
Translation	MGPGARLAALLAVLALGTGDPERAAARGDTFSALTSVARALAPERRLLGLLRRYLRGEEARLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEASENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAITDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFRAGNVSCALSLSREFLLYSPDNKRMARNVLKYERLLAESPNHVVAEAVIQRPNIPHLQTRDTYEGLCQTLGSQPTLYQIPSLYCSYETNSNAYLLLQPIRKEVIHLEPYIALYHDFVSDSEAQKIRELAEPWLQRSVVASGEKQLQVEYRISKSAWLKDTVDPKLVTLNHRIAALTGLDVRPPYAEYLQVVNYGIGGHYEPHFDHATSPSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIYANLSVPVVRHCFGGTCTGVVKGTVTHFMLAVLSWWEISGWPTSGYMSMDRNSADPAAPALKTELLAERSWWSPVAFQRSQEPKAGVGEEKAEQPPGRRPCQLCLCLANQRQGRGCYQGTLRMYI

Target ORF information:

RefSeq Version	NM_001288748.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 4-hydroxylase subunit alpha 3 (P4HA3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001288748.1

ORF Insert Sequence:

ATGGGTCCTG GGGCGCGGCT GGCGGCGCTG CTGGCGGTGC TGGCGCTCGG GACAGGAGAC 
CCAGAAAGGG CTGCGGCTCG GGGCGACACG TTCTCGGCGC TGACCAGCGT GGCGCGCGCC 
CTGGCGCCCG AGCGCCGGCT GCTGGGGCTG CTGAGGCGGT ACCTGCGCGG GGAGGAGGCG 
CGGCTGCGGG ACCTGACTAG ATTCTACGAC AAGGTACTTT CTTTGCATGA GGATTCAACA 
ACCCCTGTGG CTAACCCTCT GCTTGCATTT ACTCTCATCA AACGCCTGCA GTCTGACTGG 
AGGAATGTGG TACATAGTCT GGAGGCCAGT GAGAACATCC GAGCTCTGAA GGATGGCTAT 
GAGAAGGTGG AGCAAGACCT TCCAGCCTTT GAGGACCTTG AGGGAGCAGC AAGGGCCCTG 
ATGCGGCTGC AGGACGTGTA CATGCTCAAT GTGAAAGGCC TGGCCCGAGG TGTCTTTCAG 
AGAGTCACTG GCTCTGCCAT CACTGACCTG TACAGCCCCA AACGGCTCTT TTCTCTCACA 
GGGGATGACT GCTTCCAAGT TGGCAAGGTG GCCTATGACA TGGGGGATTA TTACCATGCC 
ATTCCATGGC TGGAGGAGGC TGTCAGTCTC TTCCGAGGAT CTTACGGAGA GTGGAAGACA 
GAGGATGAGG CAAGTCTAGA AGATGCCTTG GATCACTTGG CCTTTGCTTA TTTCCGGGCA 
GGAAATGTTT CGTGTGCCCT CAGCCTCTCT CGGGAGTTTC TTCTCTACAG CCCAGATAAT 
AAGAGGATGG CCAGGAATGT CTTGAAATAT GAAAGGCTCT TGGCAGAGAG CCCCAACCAC 
GTGGTAGCTG AGGCTGTCAT CCAGAGGCCC AATATACCCC ACCTGCAGAC CAGAGACACC 
TACGAGGGGC TATGTCAGAC CCTGGGTTCC CAGCCCACTC TCTACCAGAT CCCTAGCCTC 
TACTGTTCCT ATGAGACCAA TTCCAACGCC TACCTGCTGC TCCAGCCCAT CCGGAAGGAG 
GTCATCCACC TGGAGCCCTA CATTGCTCTC TACCATGACT TCGTCAGTGA CTCAGAGGCT 
CAGAAAATTA GAGAACTTGC AGAACCATGG CTACAGAGGT CAGTGGTGGC ATCAGGGGAG 
AAGCAGTTAC AAGTGGAGTA CCGCATCAGC AAAAGTGCCT GGCTGAAGGA CACTGTTGAC 
CCAAAACTGG TGACCCTCAA CCACCGCATT GCTGCCCTCA CAGGCCTTGA TGTCCGGCCT 
CCCTATGCAG AGTATCTGCA GGTGGTGAAC TATGGCATCG GAGGACACTA TGAGCCTCAC 
TTTGACCATG CTACGTCACC AAGCAGCCCC CTCTACAGAA TGAAGTCAGG AAACCGAGTT 
GCAACATTTA TGATCTATCT GAGCTCGGTG GAAGCTGGAG GAGCCACAGC CTTCATCTAT 
GCCAACCTCA GCGTGCCTGT GGTTAGGCAC TGTTTTGGTG GAACCTGCAC AGGAGTGGTG 
AAGGGGACAG TGACACACTT CATGCTGGCT GTCCTGTCCT GGTGGGAGAT AAGTGGGTGG 
CCAACAAGTG GATACATGAG TATGGACAGG AATTCCGCAG ACCCTGCAGC TCCAGCCCTG 
AAGACTGAAC TGTTGGCAGA GAGAAGCTGG TGGAGTCCTG TGGCTTTCCA GAGAAGCCAG 
GAGCCAAAAG CTGGGGTAGG AGAGGAGAAA GCAGAGCAGC CTCCTGGAAG AAGGCCTTGT 
CAGCTTTGTC TGTGCCTCGC AAATCAGAGG CAAGGGAGAG GTTGTTACCA GGGGACACTG 
AGAATGTACA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P4HA3 ( NM_001288748.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001275677.1 Homo sapiens prolyl 4-hydroxylase subunit alpha 3 (P4HA3), transcript variant 2, mRNA.

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CloneID	OHu06351
Clone ID Related Accession (Same CDS sequence)	NM_001288748.1 , NM_001288748.2
Accession Version	NM_001288748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	prolyl 4-hydroxylase subunit alpha-3 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300598.1 and HY188166.1. On May 31, 2019 this sequence version replaced NM_001288748.1. Transcript Variant: This variant (2) uses an alternate splice site in the central coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a longer and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK300598.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGGTCCTG GGGCGCGGCT GGCGGCGCTG CTGGCGGTGC TGGCGCTCGG GACAGGAGAC 
CCAGAAAGGG CTGCGGCTCG GGGCGACACG TTCTCGGCGC TGACCAGCGT GGCGCGCGCC 
CTGGCGCCCG AGCGCCGGCT GCTGGGGCTG CTGAGGCGGT ACCTGCGCGG GGAGGAGGCG 
CGGCTGCGGG ACCTGACTAG ATTCTACGAC AAGGTACTTT CTTTGCATGA GGATTCAACA 
ACCCCTGTGG CTAACCCTCT GCTTGCATTT ACTCTCATCA AACGCCTGCA GTCTGACTGG 
AGGAATGTGG TACATAGTCT GGAGGCCAGT GAGAACATCC GAGCTCTGAA GGATGGCTAT 
GAGAAGGTGG AGCAAGACCT TCCAGCCTTT GAGGACCTTG AGGGAGCAGC AAGGGCCCTG 
ATGCGGCTGC AGGACGTGTA CATGCTCAAT GTGAAAGGCC TGGCCCGAGG TGTCTTTCAG 
AGAGTCACTG GCTCTGCCAT CACTGACCTG TACAGCCCCA AACGGCTCTT TTCTCTCACA 
GGGGATGACT GCTTCCAAGT TGGCAAGGTG GCCTATGACA TGGGGGATTA TTACCATGCC 
ATTCCATGGC TGGAGGAGGC TGTCAGTCTC TTCCGAGGAT CTTACGGAGA GTGGAAGACA 
GAGGATGAGG CAAGTCTAGA AGATGCCTTG GATCACTTGG CCTTTGCTTA TTTCCGGGCA 
GGAAATGTTT CGTGTGCCCT CAGCCTCTCT CGGGAGTTTC TTCTCTACAG CCCAGATAAT 
AAGAGGATGG CCAGGAATGT CTTGAAATAT GAAAGGCTCT TGGCAGAGAG CCCCAACCAC 
GTGGTAGCTG AGGCTGTCAT CCAGAGGCCC AATATACCCC ACCTGCAGAC CAGAGACACC 
TACGAGGGGC TATGTCAGAC CCTGGGTTCC CAGCCCACTC TCTACCAGAT CCCTAGCCTC 
TACTGTTCCT ATGAGACCAA TTCCAACGCC TACCTGCTGC TCCAGCCCAT CCGGAAGGAG 
GTCATCCACC TGGAGCCCTA CATTGCTCTC TACCATGACT TCGTCAGTGA CTCAGAGGCT 
CAGAAAATTA GAGAACTTGC AGAACCATGG CTACAGAGGT CAGTGGTGGC ATCAGGGGAG 
AAGCAGTTAC AAGTGGAGTA CCGCATCAGC AAAAGTGCCT GGCTGAAGGA CACTGTTGAC 
CCAAAACTGG TGACCCTCAA CCACCGCATT GCTGCCCTCA CAGGCCTTGA TGTCCGGCCT 
CCCTATGCAG AGTATCTGCA GGTGGTGAAC TATGGCATCG GAGGACACTA TGAGCCTCAC 
TTTGACCATG CTACGTCACC AAGCAGCCCC CTCTACAGAA TGAAGTCAGG AAACCGAGTT 
GCAACATTTA TGATCTATCT GAGCTCGGTG GAAGCTGGAG GAGCCACAGC CTTCATCTAT 
GCCAACCTCA GCGTGCCTGT GGTTAGGCAC TGTTTTGGTG GAACCTGCAC AGGAGTGGTG 
AAGGGGACAG TGACACACTT CATGCTGGCT GTCCTGTCCT GGTGGGAGAT AAGTGGGTGG 
CCAACAAGTG GATACATGAG TATGGACAGG AATTCCGCAG ACCCTGCAGC TCCAGCCCTG 
AAGACTGAAC TGTTGGCAGA GAGAAGCTGG TGGAGTCCTG TGGCTTTCCA GAGAAGCCAG 
GAGCCAAAAG CTGGGGTAGG AGAGGAGAAA GCAGAGCAGC CTCCTGGAAG AAGGCCTTGT 
CAGCTTTGTC TGTGCCTCGC AAATCAGAGG CAAGGGAGAG GTTGTTACCA GGGGACACTG 
AGAATGTACA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001275677.1
CDS	30..1844
Translation	MGPGARLAALLAVLALGTGDPERAAARGDTFSALTSVARALAPERRLLGLLRRYLRGEEARLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEASENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAITDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFRAGNVSCALSLSREFLLYSPDNKRMARNVLKYERLLAESPNHVVAEAVIQRPNIPHLQTRDTYEGLCQTLGSQPTLYQIPSLYCSYETNSNAYLLLQPIRKEVIHLEPYIALYHDFVSDSEAQKIRELAEPWLQRSVVASGEKQLQVEYRISKSAWLKDTVDPKLVTLNHRIAALTGLDVRPPYAEYLQVVNYGIGGHYEPHFDHATSPSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIYANLSVPVVRHCFGGTCTGVVKGTVTHFMLAVLSWWEISGWPTSGYMSMDRNSADPAAPALKTELLAERSWWSPVAFQRSQEPKAGVGEEKAEQPPGRRPCQLCLCLANQRQGRGCYQGTLRMYI

Target ORF information:

RefSeq Version	NM_001288748.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 4-hydroxylase subunit alpha 3 (P4HA3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001288748.2

ORF Insert Sequence:

ATGGGTCCTG GGGCGCGGCT GGCGGCGCTG CTGGCGGTGC TGGCGCTCGG GACAGGAGAC 
CCAGAAAGGG CTGCGGCTCG GGGCGACACG TTCTCGGCGC TGACCAGCGT GGCGCGCGCC 
CTGGCGCCCG AGCGCCGGCT GCTGGGGCTG CTGAGGCGGT ACCTGCGCGG GGAGGAGGCG 
CGGCTGCGGG ACCTGACTAG ATTCTACGAC AAGGTACTTT CTTTGCATGA GGATTCAACA 
ACCCCTGTGG CTAACCCTCT GCTTGCATTT ACTCTCATCA AACGCCTGCA GTCTGACTGG 
AGGAATGTGG TACATAGTCT GGAGGCCAGT GAGAACATCC GAGCTCTGAA GGATGGCTAT 
GAGAAGGTGG AGCAAGACCT TCCAGCCTTT GAGGACCTTG AGGGAGCAGC AAGGGCCCTG 
ATGCGGCTGC AGGACGTGTA CATGCTCAAT GTGAAAGGCC TGGCCCGAGG TGTCTTTCAG 
AGAGTCACTG GCTCTGCCAT CACTGACCTG TACAGCCCCA AACGGCTCTT TTCTCTCACA 
GGGGATGACT GCTTCCAAGT TGGCAAGGTG GCCTATGACA TGGGGGATTA TTACCATGCC 
ATTCCATGGC TGGAGGAGGC TGTCAGTCTC TTCCGAGGAT CTTACGGAGA GTGGAAGACA 
GAGGATGAGG CAAGTCTAGA AGATGCCTTG GATCACTTGG CCTTTGCTTA TTTCCGGGCA 
GGAAATGTTT CGTGTGCCCT CAGCCTCTCT CGGGAGTTTC TTCTCTACAG CCCAGATAAT 
AAGAGGATGG CCAGGAATGT CTTGAAATAT GAAAGGCTCT TGGCAGAGAG CCCCAACCAC 
GTGGTAGCTG AGGCTGTCAT CCAGAGGCCC AATATACCCC ACCTGCAGAC CAGAGACACC 
TACGAGGGGC TATGTCAGAC CCTGGGTTCC CAGCCCACTC TCTACCAGAT CCCTAGCCTC 
TACTGTTCCT ATGAGACCAA TTCCAACGCC TACCTGCTGC TCCAGCCCAT CCGGAAGGAG 
GTCATCCACC TGGAGCCCTA CATTGCTCTC TACCATGACT TCGTCAGTGA CTCAGAGGCT 
CAGAAAATTA GAGAACTTGC AGAACCATGG CTACAGAGGT CAGTGGTGGC ATCAGGGGAG 
AAGCAGTTAC AAGTGGAGTA CCGCATCAGC AAAAGTGCCT GGCTGAAGGA CACTGTTGAC 
CCAAAACTGG TGACCCTCAA CCACCGCATT GCTGCCCTCA CAGGCCTTGA TGTCCGGCCT 
CCCTATGCAG AGTATCTGCA GGTGGTGAAC TATGGCATCG GAGGACACTA TGAGCCTCAC 
TTTGACCATG CTACGTCACC AAGCAGCCCC CTCTACAGAA TGAAGTCAGG AAACCGAGTT 
GCAACATTTA TGATCTATCT GAGCTCGGTG GAAGCTGGAG GAGCCACAGC CTTCATCTAT 
GCCAACCTCA GCGTGCCTGT GGTTAGGCAC TGTTTTGGTG GAACCTGCAC AGGAGTGGTG 
AAGGGGACAG TGACACACTT CATGCTGGCT GTCCTGTCCT GGTGGGAGAT AAGTGGGTGG 
CCAACAAGTG GATACATGAG TATGGACAGG AATTCCGCAG ACCCTGCAGC TCCAGCCCTG 
AAGACTGAAC TGTTGGCAGA GAGAAGCTGG TGGAGTCCTG TGGCTTTCCA GAGAAGCCAG 
GAGCCAAAAG CTGGGGTAGG AGAGGAGAAA GCAGAGCAGC CTCCTGGAAG AAGGCCTTGT 
CAGCTTTGTC TGTGCCTCGC AAATCAGAGG CAAGGGAGAG GTTGTTACCA GGGGACACTG 
AGAATGTACA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

The length of peptide substrates has a marked effect on hydroxylation by the hypoxia-inducible factor prolyl 4-hydroxylases.
The Journal of biological chemistry281(39)28712-20(2006 Sep)
Koivunen P,Hirsil? M,Kivirikko KI,Myllyharju J

Identification and characterization of a third human, rat, and mouse collagen prolyl 4-hydroxylase isoenzyme.
The Journal of biological chemistry278(48)47685-93(2003 Nov)
Kukkola L,Hieta R,Kivirikko KI,Myllyharju J

Cloning of a novel prolyl 4-hydroxylase subunit expressed in the fibrous cap of human atherosclerotic plaque.
Circulation108(5)508-11(2003 Aug)
Van Den Diepstraten C,Papay K,Bolender Z,Brown A,Pickering JG

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P4HA3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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