PPP2R3B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP2R3B
Entrez Gene ID	28227
Full Name	protein phosphatase 2 regulatory subunit B''beta
Synonyms	NYREN8,PPP2R3L,PPP2R3LY,PR48,PR70
General protein information	Preferred Names protein phosphatase 2 regulatory subunit B''beta Names serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta NY-REN-8 antigen PP2A, subunit B, PR48 isoform protein phosphatase 2 (formerly 2A), regulatory subunit B'', beta protein phosphatase 2, regulatory subunit B'', beta serine/threonine protein phosphatase 2A, 48kDa regulatory subunit B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X\|Y Map Location: Xp22.33 and Yp11.31
Summary	Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the beta subfamily of regulatory subunit B''. [provided by RefSeq, Apr 2010].
Disorder MIM:	300339

mRNA and Protein(s)

mRNA	Protein	Name
NM_013239.5	NP_037371.2	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta
XM_011546177.3	XP_011544479.1	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta isoform X1
XM_011545630.2	XP_011543932.1	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta isoform X1
XM_011545630.3	XP_011543932.1	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta isoform X1
NM_013239.4	NP_037371.2	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta
XM_011546177.2	XP_011544479.1	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PPP2R3B	NP_037371.2
Xenopus tropicalis (tropical clawed frog)	ppp2r3b	NP_001072674.1
Canis lupus familiaris (dog)	PPP2R3B	XP_003640321.1
Canis lupus familiaris (dog)	LOC607103	XP_005641024.1
Gallus gallus (chicken)	PPP2R3B	XP_416866.2
Drosophila melanogaster (fruit fly)	CG4733	NP_001247197.1
Arabidopsis thaliana (thale cress)	AT5G44090	NP_199222.1
Danio rerio (zebrafish)	ppp2r3b	XP_005167917.1
Arabidopsis thaliana (thale cress)	AT1G03960	NP_171892.2

Gene Ontology
Bibliography

Related articles in PubMed

The protein phosphatase 2A regulatory subunit PR70 is a gonosomal melanoma tumor suppressor gene.
van Kempen LC, Redpath M, Elchebly M, Klein KO, Papadakis AI, Wilmott JS, Scolyer RA, Edqvist PH, Pont?n F, Schadendorf D, van Rijk AF, Michiels S, Dumay A, Helbling-Leclerc A, Dessen P, Wouters J, Stass M, Greenwood CM, Ghanem GE, van den Oord J, Feunteun J, Spatz A
Science translational medicine8(369)369ra177(2016 Dec)

Characterization and Genetic Analyses of New Genes Coding for NOD2 Interacting Proteins.
Thi?baut R, Esmiol S, Lecine P, Mahfouz B, Hermant A, Nicoletti C, Parnis S, Perroy J, Borg JP, Pascoe L, Hugot JP, Ollendorff V
PloS one11(11)e0165420(2016)

Structural and biochemical characterization of human PR70 in isolation and in complex with the scaffolding subunit of protein phosphatase 2A.
Dovega R, Tsutakawa S, Quistgaard EM, Anandapadamanaban M, L?w C, Nordlund P
PloS one9(7)e101846(2014)

Next-generation sequencing to generate interactome datasets.
Yu H, Tardivo L, Tam S, Weiner E, Gebreab F, Fan C, Svrzikapa N, Hirozane-Kishikawa T, Rietman E, Yang X, Sahalie J, Salehi-Ashtiani K, Hao T, Cusick ME, Hill DE, Roth FP, Braun P, Vidal M
Nature methods8(6)478-80(2011 Jun)

Identification and characterization of B"-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues.
Stevens I, Janssens V, Martens E, Dilworth S, Goris J, Van Hoof C
European journal of biochemistry270(2)376-87(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PPP2R3B codes for the PR70 protein, a regulatory substrate-recognizing subunit of protein phosphatase 2A. PR70 decreased melanoma growth by negatively interfering with DNA replication and cell cycle progression through its role in stabilizing CDC6-chromatin licensing and CDT1 interaction
Title: The protein phosphatase 2A regulatory subunit PR70 is a gonosomal melanoma tumor suppressor gene.

Results show the crystal structure of PR48/PR70, at 2.0 A degrees resolution with two domain elongated structure and two Ca2+ binding EF-hands Scattering data.
Title: Structural and biochemical characterization of human PR70 in isolation and in complex with the scaffolding subunit of protein phosphatase 2A.

The following PPP2R3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP2R3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21915	NM_013239.5 Latest version!	Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
OHu67259	XM_011546177.3 Latest version!	Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu67259	XM_011545630.3 Latest version!	Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OHu67259	XM_011545630.2	Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu21915	NM_013239.4	Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
OHu67259	XM_011546177.2	Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
Hide Old Accession Versions >>

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Reference Sequences (Refseq)

PPP2R3B ( NM_013239.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_037371.2 Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), mRNA.

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CloneID	OHu21915
Clone ID Related Accession (Same CDS sequence)	NM_013239.5 , NM_013239.4
Accession Version	NM_013239.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA061573.1, BK000521.1, BM715684.1, AL954664.18 and BM666129.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_013239.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BK000521.1, BC063429.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000390665.9/ ENSP00000375080.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGCCCG GCAAAGTGCT GCAGCCGGTC CTGAAGATGA AGGTGGACGA GCTGTTCCTG 
TACTGGCTCA GCGAGGCCAG CACGCAGCGG ATGCTGCAGG ACTGCCTGCG CCGGATCAAG 
GCGCCCGGGC GGGACCAGCC GACCCCGGGG GACGGGGAGC AGCCCGGGGC CTGGCCCACA 
GCCCCGCTCG CCGCCCCCCG GCCCAGCGGG CTCGAACCCC CGGGAACCCC CGGGCCGGGC 
CCTGCGCTGC CCCTGGGCGC CGCCTCCAGC CCCAGGAACG CGCCCCACGT TCGAGGCACC 
CGTAGATCCG CAGGGACGAG AGTAGTTCAG ACACGGAAAG AAGAGCCTCT GCCCCCGGCC 
ACGAGCCAAA GCATTCCGAC CTTCTACTTC CCCAGAGGAC GCCCGCAGGA CTCCGTCAAC 
GTGGATGCCG TCATCAGCAA GATCGAGAGC ACCTTCGCCC GGTTCCCCCA CGAGAGGGCC 
ACCATGGATG ACATGGGCCT GGTGGCCAAG GCCTGCGGCT GCCCCCTCTA CTGGAAGGGG 
CCGCTCTTCT ATGGCGCCGG CGGGGAGCGC ACGGGCTCCG TGTCCGTCCA CAAGTTCGTC 
GCCATGTGGA GAAAAATCCT CCAGAACTGC CACGACGACG CGGCCAAGTT CGTCCATCTG 
CTCATGAGCC CCGGCTGCAA CTACCTGGTG CAGGAGGACT TTGTCCCCTT CTTGCAGGAC 
GTGGTGAACA CGCACCCGGG GCTGTCGTTC CTGAAGGAGG CGTCCGAGTT CCACTCGCGC 
TACATCACCA CGGTCATCCA GCGGATCTTC TACGCCGTGA ACCGGTCCTG GTCCGGCAGG 
ATCACCTGCG CCGAGCTGCG GAGGAGCTCC TTCCTGCAGA ATGTGGCGCT GCTGGAGGAG 
GAGGCGGACA TCAACCAGCT GACCGAATTC TTCTCGTACG AGCATTTCTA CGTCATCTAC 
TGCAAGTTCT GGGAGCTGGA CACGGACCAC GACCTGCTCA TCGACGCGGA CGACCTGGCG 
CGGCACAATG ACCACGCCCT TTCTACCAAG ATGATAGACA GGATCTTCTC AGGAGCAGTC 
ACACGAGGCA GAAAAGTGCA GAAGGAAGGG AAGATCAGCT ATGCCGACTT TGTCTGGTTT 
TTGATCTCTG AGGAAGACAA AAAAACACCG ACCAGCATCG AGTACTGGTT CCGCTGCATG 
GACCTGGACG GGGACGGCGC CCTGTCCATG TTCGAGCTCG AGTACTTCTA CGAGGAGCAG 
TGCCGAAGGC TGGACAGCAT GGCCATCGAG GCCCTGCCCT TCCAGGACTG CCTCTGCCAG 
ATGCTGGACC TGGTCAAGCC GAGGACTGAA GGGAAGATCA CGCTGCAGGA CCTGAAGCGC 
TGCAAGCTGG CTAACGTCTT CTTCGACACC TTCTTCAACA TCGAGAAGTA CCTCGACCAC 
GAGCAGAAAG AGCAGATCTC CCTGCTCAGG GACGGTGACA GCGGCGGCCC CGAGCTCTCG 
GACTGGGAGA AGTACGCGGC CGAGGAGTAC GACATCCTGG TGGCCGAGGA GACTGCGGGA 
GAGCCCTGGG AGGACGGGTT CGAGGCCGAG CTCAGCCCTG TGGAGCAGAA GCTGAGTGCG 
CTGCGCTCCC CGCTGGCCCA GAGGCCCTTC TTCGAGGCGC CCTCACCGCT GGGCGCCGTG 
GACCTGTACG AGTACGCATG CGGGGACGAG GACCTGGAGC CGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037371.2
CDS	217..1944
Translation	MPPGKVLQPVLKMKVDELFLYWLSEASTQRMLQDCLRRIKAPGRDQPTPGDGEQPGAWPTAPLAAPRPSGLEPPGTPGPGPALPLGAASSPRNAPHVRGTRRSAGTRVVQTRKEEPLPPATSQSIPTFYFPRGRPQDSVNVDAVISKIESTFARFPHERATMDDMGLVAKACGCPLYWKGPLFYGAGGERTGSVSVHKFVAMWRKILQNCHDDAAKFVHLLMSPGCNYLVQEDFVPFLQDVVNTHPGLSFLKEASEFHSRYITTVIQRIFYAVNRSWSGRITCAELRRSSFLQNVALLEEEADINQLTEFFSYEHFYVIYCKFWELDTDHDLLIDADDLARHNDHALSTKMIDRIFSGAVTRGRKVQKEGKISYADFVWFLISEEDKKTPTSIEYWFRCMDLDGDGALSMFELEYFYEEQCRRLDSMAIEALPFQDCLCQMLDLVKPRTEGKITLQDLKRCKLANVFFDTFFNIEKYLDHEQKEQISLLRDGDSGGPELSDWEKYAAEEYDILVAEETAGEPWEDGFEAELSPVEQKLSALRSPLAQRPFFEAPSPLGAVDLYEYACGDEDLEPL

Target ORF information:

RefSeq Version	NM_013239.5
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013239.5

ORF Insert Sequence:

ATGCCGCCCG GCAAAGTGCT GCAGCCGGTC CTGAAGATGA AGGTGGACGA GCTGTTCCTG 
TACTGGCTCA GCGAGGCCAG CACGCAGCGG ATGCTGCAGG ACTGCCTGCG CCGGATCAAG 
GCGCCCGGGC GGGACCAGCC GACCCCGGGG GACGGGGAGC AGCCCGGGGC CTGGCCCACA 
GCCCCGCTCG CCGCCCCCCG GCCCAGCGGG CTCGAACCCC CGGGAACCCC CGGGCCGGGC 
CCTGCGCTGC CCCTGGGCGC CGCCTCCAGC CCCAGGAACG CGCCCCACGT TCGAGGCACC 
CGTAGATCCG CAGGGACGAG AGTAGTTCAG ACACGGAAAG AAGAGCCTCT GCCCCCGGCC 
ACGAGCCAAA GCATTCCGAC CTTCTACTTC CCCAGAGGAC GCCCGCAGGA CTCCGTCAAC 
GTGGATGCCG TCATCAGCAA GATCGAGAGC ACCTTCGCCC GGTTCCCCCA CGAGAGGGCC 
ACCATGGATG ACATGGGCCT GGTGGCCAAG GCCTGCGGCT GCCCCCTCTA CTGGAAGGGG 
CCGCTCTTCT ATGGCGCCGG CGGGGAGCGC ACGGGCTCCG TGTCCGTCCA CAAGTTCGTC 
GCCATGTGGA GAAAAATCCT CCAGAACTGC CACGACGACG CGGCCAAGTT CGTCCATCTG 
CTCATGAGCC CCGGCTGCAA CTACCTGGTG CAGGAGGACT TTGTCCCCTT CTTGCAGGAC 
GTGGTGAACA CGCACCCGGG GCTGTCGTTC CTGAAGGAGG CGTCCGAGTT CCACTCGCGC 
TACATCACCA CGGTCATCCA GCGGATCTTC TACGCCGTGA ACCGGTCCTG GTCCGGCAGG 
ATCACCTGCG CCGAGCTGCG GAGGAGCTCC TTCCTGCAGA ATGTGGCGCT GCTGGAGGAG 
GAGGCGGACA TCAACCAGCT GACCGAATTC TTCTCGTACG AGCATTTCTA CGTCATCTAC 
TGCAAGTTCT GGGAGCTGGA CACGGACCAC GACCTGCTCA TCGACGCGGA CGACCTGGCG 
CGGCACAATG ACCACGCCCT TTCTACCAAG ATGATAGACA GGATCTTCTC AGGAGCAGTC 
ACACGAGGCA GAAAAGTGCA GAAGGAAGGG AAGATCAGCT ATGCCGACTT TGTCTGGTTT 
TTGATCTCTG AGGAAGACAA AAAAACACCG ACCAGCATCG AGTACTGGTT CCGCTGCATG 
GACCTGGACG GGGACGGCGC CCTGTCCATG TTCGAGCTCG AGTACTTCTA CGAGGAGCAG 
TGCCGAAGGC TGGACAGCAT GGCCATCGAG GCCCTGCCCT TCCAGGACTG CCTCTGCCAG 
ATGCTGGACC TGGTCAAGCC GAGGACTGAA GGGAAGATCA CGCTGCAGGA CCTGAAGCGC 
TGCAAGCTGG CTAACGTCTT CTTCGACACC TTCTTCAACA TCGAGAAGTA CCTCGACCAC 
GAGCAGAAAG AGCAGATCTC CCTGCTCAGG GACGGTGACA GCGGCGGCCC CGAGCTCTCG 
GACTGGGAGA AGTACGCGGC CGAGGAGTAC GACATCCTGG TGGCCGAGGA GACTGCGGGA 
GAGCCCTGGG AGGACGGGTT CGAGGCCGAG CTCAGCCCTG TGGAGCAGAA GCTGAGTGCG 
CTGCGCTCCC CGCTGGCCCA GAGGCCCTTC TTCGAGGCGC CCTCACCGCT GGGCGCCGTG 
GACCTGTACG AGTACGCATG CGGGGACGAG GACCTGGAGC CGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP2R3B ( NM_013239.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_037371.2 Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), mRNA.

Price & Availability↑

CloneID	OHu21915
Clone ID Related Accession (Same CDS sequence)	NM_013239.5 , NM_013239.4
Accession Version	NM_013239.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA061573.1, BK000521.1, BM715684.1, AL954664.18 and BM666129.1. This sequence is a reference standard in the RefSeqGene project. On Apr 10, 2010 this sequence version replaced NM_013239.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.161668.1, BK000521.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGCCCG GCAAAGTGCT GCAGCCGGTC CTGAAGATGA AGGTGGACGA GCTGTTCCTG 
TACTGGCTCA GCGAGGCCAG CACGCAGCGG ATGCTGCAGG ACTGCCTGCG CCGGATCAAG 
GCGCCCGGGC GGGACCAGCC GACCCCGGGG GACGGGGAGC AGCCCGGGGC CTGGCCCACA 
GCCCCGCTCG CCGCCCCCCG GCCCAGCGGG CTCGAACCCC CGGGAACCCC CGGGCCGGGC 
CCTGCGCTGC CCCTGGGCGC CGCCTCCAGC CCCAGGAACG CGCCCCACGT TCGAGGCACC 
CGTAGATCCG CAGGGACGAG AGTAGTTCAG ACACGGAAAG AAGAGCCTCT GCCCCCGGCC 
ACGAGCCAAA GCATTCCGAC CTTCTACTTC CCCAGAGGAC GCCCGCAGGA CTCCGTCAAC 
GTGGATGCCG TCATCAGCAA GATCGAGAGC ACCTTCGCCC GGTTCCCCCA CGAGAGGGCC 
ACCATGGATG ACATGGGCCT GGTGGCCAAG GCCTGCGGCT GCCCCCTCTA CTGGAAGGGG 
CCGCTCTTCT ATGGCGCCGG CGGGGAGCGC ACGGGCTCCG TGTCCGTCCA CAAGTTCGTC 
GCCATGTGGA GAAAAATCCT CCAGAACTGC CACGACGACG CGGCCAAGTT CGTCCATCTG 
CTCATGAGCC CCGGCTGCAA CTACCTGGTG CAGGAGGACT TTGTCCCCTT CTTGCAGGAC 
GTGGTGAACA CGCACCCGGG GCTGTCGTTC CTGAAGGAGG CGTCCGAGTT CCACTCGCGC 
TACATCACCA CGGTCATCCA GCGGATCTTC TACGCCGTGA ACCGGTCCTG GTCCGGCAGG 
ATCACCTGCG CCGAGCTGCG GAGGAGCTCC TTCCTGCAGA ATGTGGCGCT GCTGGAGGAG 
GAGGCGGACA TCAACCAGCT GACCGAATTC TTCTCGTACG AGCATTTCTA CGTCATCTAC 
TGCAAGTTCT GGGAGCTGGA CACGGACCAC GACCTGCTCA TCGACGCGGA CGACCTGGCG 
CGGCACAATG ACCACGCCCT TTCTACCAAG ATGATAGACA GGATCTTCTC AGGAGCAGTC 
ACACGAGGCA GAAAAGTGCA GAAGGAAGGG AAGATCAGCT ATGCCGACTT TGTCTGGTTT 
TTGATCTCTG AGGAAGACAA AAAAACACCG ACCAGCATCG AGTACTGGTT CCGCTGCATG 
GACCTGGACG GGGACGGCGC CCTGTCCATG TTCGAGCTCG AGTACTTCTA CGAGGAGCAG 
TGCCGAAGGC TGGACAGCAT GGCCATCGAG GCCCTGCCCT TCCAGGACTG CCTCTGCCAG 
ATGCTGGACC TGGTCAAGCC GAGGACTGAA GGGAAGATCA CGCTGCAGGA CCTGAAGCGC 
TGCAAGCTGG CTAACGTCTT CTTCGACACC TTCTTCAACA TCGAGAAGTA CCTCGACCAC 
GAGCAGAAAG AGCAGATCTC CCTGCTCAGG GACGGTGACA GCGGCGGCCC CGAGCTCTCG 
GACTGGGAGA AGTACGCGGC CGAGGAGTAC GACATCCTGG TGGCCGAGGA GACTGCGGGA 
GAGCCCTGGG AGGACGGGTT CGAGGCCGAG CTCAGCCCTG TGGAGCAGAA GCTGAGTGCG 
CTGCGCTCCC CGCTGGCCCA GAGGCCCTTC TTCGAGGCGC CCTCACCGCT GGGCGCCGTG 
GACCTGTACG AGTACGCATG CGGGGACGAG GACCTGGAGC CGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037371.2
CDS	265..1992
Translation	MPPGKVLQPVLKMKVDELFLYWLSEASTQRMLQDCLRRIKAPGRDQPTPGDGEQPGAWPTAPLAAPRPSGLEPPGTPGPGPALPLGAASSPRNAPHVRGTRRSAGTRVVQTRKEEPLPPATSQSIPTFYFPRGRPQDSVNVDAVISKIESTFARFPHERATMDDMGLVAKACGCPLYWKGPLFYGAGGERTGSVSVHKFVAMWRKILQNCHDDAAKFVHLLMSPGCNYLVQEDFVPFLQDVVNTHPGLSFLKEASEFHSRYITTVIQRIFYAVNRSWSGRITCAELRRSSFLQNVALLEEEADINQLTEFFSYEHFYVIYCKFWELDTDHDLLIDADDLARHNDHALSTKMIDRIFSGAVTRGRKVQKEGKISYADFVWFLISEEDKKTPTSIEYWFRCMDLDGDGALSMFELEYFYEEQCRRLDSMAIEALPFQDCLCQMLDLVKPRTEGKITLQDLKRCKLANVFFDTFFNIEKYLDHEQKEQISLLRDGDSGGPELSDWEKYAAEEYDILVAEETAGEPWEDGFEAELSPVEQKLSALRSPLAQRPFFEAPSPLGAVDLYEYACGDEDLEPL

Target ORF information:

RefSeq Version	NM_013239.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013239.4

ORF Insert Sequence:

ATGCCGCCCG GCAAAGTGCT GCAGCCGGTC CTGAAGATGA AGGTGGACGA GCTGTTCCTG 
TACTGGCTCA GCGAGGCCAG CACGCAGCGG ATGCTGCAGG ACTGCCTGCG CCGGATCAAG 
GCGCCCGGGC GGGACCAGCC GACCCCGGGG GACGGGGAGC AGCCCGGGGC CTGGCCCACA 
GCCCCGCTCG CCGCCCCCCG GCCCAGCGGG CTCGAACCCC CGGGAACCCC CGGGCCGGGC 
CCTGCGCTGC CCCTGGGCGC CGCCTCCAGC CCCAGGAACG CGCCCCACGT TCGAGGCACC 
CGTAGATCCG CAGGGACGAG AGTAGTTCAG ACACGGAAAG AAGAGCCTCT GCCCCCGGCC 
ACGAGCCAAA GCATTCCGAC CTTCTACTTC CCCAGAGGAC GCCCGCAGGA CTCCGTCAAC 
GTGGATGCCG TCATCAGCAA GATCGAGAGC ACCTTCGCCC GGTTCCCCCA CGAGAGGGCC 
ACCATGGATG ACATGGGCCT GGTGGCCAAG GCCTGCGGCT GCCCCCTCTA CTGGAAGGGG 
CCGCTCTTCT ATGGCGCCGG CGGGGAGCGC ACGGGCTCCG TGTCCGTCCA CAAGTTCGTC 
GCCATGTGGA GAAAAATCCT CCAGAACTGC CACGACGACG CGGCCAAGTT CGTCCATCTG 
CTCATGAGCC CCGGCTGCAA CTACCTGGTG CAGGAGGACT TTGTCCCCTT CTTGCAGGAC 
GTGGTGAACA CGCACCCGGG GCTGTCGTTC CTGAAGGAGG CGTCCGAGTT CCACTCGCGC 
TACATCACCA CGGTCATCCA GCGGATCTTC TACGCCGTGA ACCGGTCCTG GTCCGGCAGG 
ATCACCTGCG CCGAGCTGCG GAGGAGCTCC TTCCTGCAGA ATGTGGCGCT GCTGGAGGAG 
GAGGCGGACA TCAACCAGCT GACCGAATTC TTCTCGTACG AGCATTTCTA CGTCATCTAC 
TGCAAGTTCT GGGAGCTGGA CACGGACCAC GACCTGCTCA TCGACGCGGA CGACCTGGCG 
CGGCACAATG ACCACGCCCT TTCTACCAAG ATGATAGACA GGATCTTCTC AGGAGCAGTC 
ACACGAGGCA GAAAAGTGCA GAAGGAAGGG AAGATCAGCT ATGCCGACTT TGTCTGGTTT 
TTGATCTCTG AGGAAGACAA AAAAACACCG ACCAGCATCG AGTACTGGTT CCGCTGCATG 
GACCTGGACG GGGACGGCGC CCTGTCCATG TTCGAGCTCG AGTACTTCTA CGAGGAGCAG 
TGCCGAAGGC TGGACAGCAT GGCCATCGAG GCCCTGCCCT TCCAGGACTG CCTCTGCCAG 
ATGCTGGACC TGGTCAAGCC GAGGACTGAA GGGAAGATCA CGCTGCAGGA CCTGAAGCGC 
TGCAAGCTGG CTAACGTCTT CTTCGACACC TTCTTCAACA TCGAGAAGTA CCTCGACCAC 
GAGCAGAAAG AGCAGATCTC CCTGCTCAGG GACGGTGACA GCGGCGGCCC CGAGCTCTCG 
GACTGGGAGA AGTACGCGGC CGAGGAGTAC GACATCCTGG TGGCCGAGGA GACTGCGGGA 
GAGCCCTGGG AGGACGGGTT CGAGGCCGAG CTCAGCCCTG TGGAGCAGAA GCTGAGTGCG 
CTGCGCTCCC CGCTGGCCCA GAGGCCCTTC TTCGAGGCGC CCTCACCGCT GGGCGCCGTG 
GACCTGTACG AGTACGCATG CGGGGACGAG GACCTGGAGC CGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP2R3B ( XM_011546177.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011544479.1 Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), transcript variant X1, mRNA.

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CloneID	OHu67259
Clone ID Related Accession (Same CDS sequence)	XM_011546177.3 , XM_011545630.2 , XM_011545630.3 , XM_011546177.2
Accession Version	XM_011546177.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011546177.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCCG GCAAAGTGCT GCAGCCGGTC CTGAAGATGA AGGTGGACGA GCTGTTCCTG 
TACTGGCTCA GCGAGGCCAG CACGCAGCGG ATGCTGCAGG ACTGCCTGCG CCGGATCAAG 
GCGCCCGGGC GGGACCAGCC GACCCCGGGG GACGGGGAGC AGCCCGGGGC CTGGCCCACA 
GCCCCGCTCG CCGCCCCCCG GCCCAGCGGG CTCGAACCCC CGGGAACCCC CGGGCCGGGC 
CCTGCGCTGC CCCTGGGCGC CGCCTCCAGC CCCAGGAACG CGCCCCACGT TCGAGGCACC 
CGTAGATCCG CAGGGACGAG AGTAGTTCAG ACACGGAAAG AAGAGCCTCT GCCCCCGGCC 
ACGAGCCAAA GCATTCCGAC CTTCTACTTC CCCAGAGGAC GCCCGCAGGA CTCCGTCAAC 
GTGGATGCCG TCATCAGCAA GATCGAGAGC ACCTTCGCCC GGTTCCCCCA CGAGAGGGCC 
ACCATGGATG ACATGGGCCT GGTGGCCAAG GCCTGCGGCT GCCCCCTCTA CTGGAAGGGG 
CCGCTCTTCT ATGGCGCCGG CGGGGAGCGC ACGGGCTCCG TGTCCGTCCA CAAGTTCGTC 
GCCATGTGGA GAAAAATCCT CCAGAACTGC CACGACGACG CGGCCAAGTT CGTCCATCTG 
CTCATGAGCC CCGGCTGCAA CTACCTGGTG CAGGAGGACT TTGTCCCCTT CTTGCAGGAC 
GTGGTGAACA CGCACCCGGG GCTGTCGTTC CTGAAGGAGG CGTCCGAGTT CCACTCGCGC 
TACATCACCA CGGTCATCCA GCGGATCTTC TACGCCGTGA ACCGGTCCTG GTCCGGCAGG 
ATCACCTGCG CCGAGCTGCG GAGGAGCTCC TTCCTGCAGA ATGTGGCGCT GCTGGAGGAG 
GAGGCGGACA TCAACCAGCT GACCGAATTC TTCTCGTACG AGCATTTCTA CGTCATCTAC 
TGCAAGTTCT GGGAGCTGGA CACGGACCAC GACCTGCTCA TCGACGCGGA CGACCTGGCG 
CGGCACAATG ACCACGCCCT TTCTACCAAG ATGATAGACA GGATCTTCTC AGGAGCAGTC 
ACACGAGGCA GAAAAGTGCA GAAGGAAGGG AAGATCAGCT ATGCCGACTT TGTCTGGTTT 
TTGATCTCTG AGGAAGACAA AAAAACACCG ACCAGCATCG AGTACTGGTT CCGCTGCATG 
GACCTGGACG GGGACGGCGC CCTGTCCATG TTCGAGCTCG AGTACTTCTA CGAGGAGCAG 
TGCCGAAGGC TGGACAGCAT GGCCATCGAG GCCCTGCCCT TCCAGGACTG CCTCTGCCAG 
ATGCTGGACC TGGTCAAGCC GAGGACTGAA GGGAAGATCA CGCTGCAGGA CCTGAAGCGC 
TGCAAGCTGG CTAACGTCTT CTTCGACACC TTCTTCAACA TCGAGAAGTA CCTCGACCAC 
GAGCAGAAAG AGCAGATCTC CCTGCTCAGG GACGGTGACA GCGGCGGCCC CGAGCTCTCG 
GACTGGGAGA AGTACGCGGC CGAGGAGTAC GACATCCTGG TGGCCGAGGA GACTGCGGGA 
GAGCCCTGGG AGGACGGAGA TGGGGTTTCA CCGTGTTGCC CAGGTTGGTC TTGGATGCCT 
GAGCTCGAGG GTTCTCCCCG CCTCGGTCCC CCAACGTGCC GGAATTACAG GTGTGAGCCC 
CTGCCCAGCC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011544479.1
CDS	260..1957
Translation	MPPGKVLQPVLKMKVDELFLYWLSEASTQRMLQDCLRRIKAPGRDQPTPGDGEQPGAWPTAPLAAPRPSGLEPPGTPGPGPALPLGAASSPRNAPHVRGTRRSAGTRVVQTRKEEPLPPATSQSIPTFYFPRGRPQDSVNVDAVISKIESTFARFPHERATMDDMGLVAKACGCPLYWKGPLFYGAGGERTGSVSVHKFVAMWRKILQNCHDDAAKFVHLLMSPGCNYLVQEDFVPFLQDVVNTHPGLSFLKEASEFHSRYITTVIQRIFYAVNRSWSGRITCAELRRSSFLQNVALLEEEADINQLTEFFSYEHFYVIYCKFWELDTDHDLLIDADDLARHNDHALSTKMIDRIFSGAVTRGRKVQKEGKISYADFVWFLISEEDKKTPTSIEYWFRCMDLDGDGALSMFELEYFYEEQCRRLDSMAIEALPFQDCLCQMLDLVKPRTEGKITLQDLKRCKLANVFFDTFFNIEKYLDHEQKEQISLLRDGDSGGPELSDWEKYAAEEYDILVAEETAGEPWEDGDGVSPCCPGWSWMPELEGSPRLGPPTCRNYRCEPLPSLE

Target ORF information:

RefSeq Version	XM_011546177.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011546177.3

ORF Insert Sequence:

ATGCCGCCCG GCAAAGTGCT GCAGCCGGTC CTGAAGATGA AGGTGGACGA GCTGTTCCTG 
TACTGGCTCA GCGAGGCCAG CACGCAGCGG ATGCTGCAGG ACTGCCTGCG CCGGATCAAG 
GCGCCCGGGC GGGACCAGCC GACCCCGGGG GACGGGGAGC AGCCCGGGGC CTGGCCCACA 
GCCCCGCTCG CCGCCCCCCG GCCCAGCGGG CTCGAACCCC CGGGAACCCC CGGGCCGGGC 
CCTGCGCTGC CCCTGGGCGC CGCCTCCAGC CCCAGGAACG CGCCCCACGT TCGAGGCACC 
CGTAGATCCG CAGGGACGAG AGTAGTTCAG ACACGGAAAG AAGAGCCTCT GCCCCCGGCC 
ACGAGCCAAA GCATTCCGAC CTTCTACTTC CCCAGAGGAC GCCCGCAGGA CTCCGTCAAC 
GTGGATGCCG TCATCAGCAA GATCGAGAGC ACCTTCGCCC GGTTCCCCCA CGAGAGGGCC 
ACCATGGATG ACATGGGCCT GGTGGCCAAG GCCTGCGGCT GCCCCCTCTA CTGGAAGGGG 
CCGCTCTTCT ATGGCGCCGG CGGGGAGCGC ACGGGCTCCG TGTCCGTCCA CAAGTTCGTC 
GCCATGTGGA GAAAAATCCT CCAGAACTGC CACGACGACG CGGCCAAGTT CGTCCATCTG 
CTCATGAGCC CCGGCTGCAA CTACCTGGTG CAGGAGGACT TTGTCCCCTT CTTGCAGGAC 
GTGGTGAACA CGCACCCGGG GCTGTCGTTC CTGAAGGAGG CGTCCGAGTT CCACTCGCGC 
TACATCACCA CGGTCATCCA GCGGATCTTC TACGCCGTGA ACCGGTCCTG GTCCGGCAGG 
ATCACCTGCG CCGAGCTGCG GAGGAGCTCC TTCCTGCAGA ATGTGGCGCT GCTGGAGGAG 
GAGGCGGACA TCAACCAGCT GACCGAATTC TTCTCGTACG AGCATTTCTA CGTCATCTAC 
TGCAAGTTCT GGGAGCTGGA CACGGACCAC GACCTGCTCA TCGACGCGGA CGACCTGGCG 
CGGCACAATG ACCACGCCCT TTCTACCAAG ATGATAGACA GGATCTTCTC AGGAGCAGTC 
ACACGAGGCA GAAAAGTGCA GAAGGAAGGG AAGATCAGCT ATGCCGACTT TGTCTGGTTT 
TTGATCTCTG AGGAAGACAA AAAAACACCG ACCAGCATCG AGTACTGGTT CCGCTGCATG 
GACCTGGACG GGGACGGCGC CCTGTCCATG TTCGAGCTCG AGTACTTCTA CGAGGAGCAG 
TGCCGAAGGC TGGACAGCAT GGCCATCGAG GCCCTGCCCT TCCAGGACTG CCTCTGCCAG 
ATGCTGGACC TGGTCAAGCC GAGGACTGAA GGGAAGATCA CGCTGCAGGA CCTGAAGCGC 
TGCAAGCTGG CTAACGTCTT CTTCGACACC TTCTTCAACA TCGAGAAGTA CCTCGACCAC 
GAGCAGAAAG AGCAGATCTC CCTGCTCAGG GACGGTGACA GCGGCGGCCC CGAGCTCTCG 
GACTGGGAGA AGTACGCGGC CGAGGAGTAC GACATCCTGG TGGCCGAGGA GACTGCGGGA 
GAGCCCTGGG AGGACGGAGA TGGGGTTTCA CCGTGTTGCC CAGGTTGGTC TTGGATGCCT 
GAGCTCGAGG GTTCTCCCCG CCTCGGTCCC CCAACGTGCC GGAATTACAG GTGTGAGCCC 
CTGCCCAGCC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP2R3B ( XM_011545630.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543932.1 Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), transcript variant X1, mRNA.

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CloneID	OHu67259
Clone ID Related Accession (Same CDS sequence)	XM_011546177.3 , XM_011545630.2 , XM_011545630.3 , XM_011546177.2
Accession Version	XM_011545630.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000024.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545630.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCCG GCAAAGTGCT GCAGCCGGTC CTGAAGATGA AGGTGGACGA GCTGTTCCTG 
TACTGGCTCA GCGAGGCCAG CACGCAGCGG ATGCTGCAGG ACTGCCTGCG CCGGATCAAG 
GCGCCCGGGC GGGACCAGCC GACCCCGGGG GACGGGGAGC AGCCCGGGGC CTGGCCCACA 
GCCCCGCTCG CCGCCCCCCG GCCCAGCGGG CTCGAACCCC CGGGAACCCC CGGGCCGGGC 
CCTGCGCTGC CCCTGGGCGC CGCCTCCAGC CCCAGGAACG CGCCCCACGT TCGAGGCACC 
CGTAGATCCG CAGGGACGAG AGTAGTTCAG ACACGGAAAG AAGAGCCTCT GCCCCCGGCC 
ACGAGCCAAA GCATTCCGAC CTTCTACTTC CCCAGAGGAC GCCCGCAGGA CTCCGTCAAC 
GTGGATGCCG TCATCAGCAA GATCGAGAGC ACCTTCGCCC GGTTCCCCCA CGAGAGGGCC 
ACCATGGATG ACATGGGCCT GGTGGCCAAG GCCTGCGGCT GCCCCCTCTA CTGGAAGGGG 
CCGCTCTTCT ATGGCGCCGG CGGGGAGCGC ACGGGCTCCG TGTCCGTCCA CAAGTTCGTC 
GCCATGTGGA GAAAAATCCT CCAGAACTGC CACGACGACG CGGCCAAGTT CGTCCATCTG 
CTCATGAGCC CCGGCTGCAA CTACCTGGTG CAGGAGGACT TTGTCCCCTT CTTGCAGGAC 
GTGGTGAACA CGCACCCGGG GCTGTCGTTC CTGAAGGAGG CGTCCGAGTT CCACTCGCGC 
TACATCACCA CGGTCATCCA GCGGATCTTC TACGCCGTGA ACCGGTCCTG GTCCGGCAGG 
ATCACCTGCG CCGAGCTGCG GAGGAGCTCC TTCCTGCAGA ATGTGGCGCT GCTGGAGGAG 
GAGGCGGACA TCAACCAGCT GACCGAATTC TTCTCGTACG AGCATTTCTA CGTCATCTAC 
TGCAAGTTCT GGGAGCTGGA CACGGACCAC GACCTGCTCA TCGACGCGGA CGACCTGGCG 
CGGCACAATG ACCACGCCCT TTCTACCAAG ATGATAGACA GGATCTTCTC AGGAGCAGTC 
ACACGAGGCA GAAAAGTGCA GAAGGAAGGG AAGATCAGCT ATGCCGACTT TGTCTGGTTT 
TTGATCTCTG AGGAAGACAA AAAAACACCG ACCAGCATCG AGTACTGGTT CCGCTGCATG 
GACCTGGACG GGGACGGCGC CCTGTCCATG TTCGAGCTCG AGTACTTCTA CGAGGAGCAG 
TGCCGAAGGC TGGACAGCAT GGCCATCGAG GCCCTGCCCT TCCAGGACTG CCTCTGCCAG 
ATGCTGGACC TGGTCAAGCC GAGGACTGAA GGGAAGATCA CGCTGCAGGA CCTGAAGCGC 
TGCAAGCTGG CTAACGTCTT CTTCGACACC TTCTTCAACA TCGAGAAGTA CCTCGACCAC 
GAGCAGAAAG AGCAGATCTC CCTGCTCAGG GACGGTGACA GCGGCGGCCC CGAGCTCTCG 
GACTGGGAGA AGTACGCGGC CGAGGAGTAC GACATCCTGG TGGCCGAGGA GACTGCGGGA 
GAGCCCTGGG AGGACGGAGA TGGGGTTTCA CCGTGTTGCC CAGGTTGGTC TTGGATGCCT 
GAGCTCGAGG GTTCTCCCCG CCTCGGTCCC CCAACGTGCC GGAATTACAG GTGTGAGCCC 
CTGCCCAGCC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543932.1
CDS	265..1962
Translation	MPPGKVLQPVLKMKVDELFLYWLSEASTQRMLQDCLRRIKAPGRDQPTPGDGEQPGAWPTAPLAAPRPSGLEPPGTPGPGPALPLGAASSPRNAPHVRGTRRSAGTRVVQTRKEEPLPPATSQSIPTFYFPRGRPQDSVNVDAVISKIESTFARFPHERATMDDMGLVAKACGCPLYWKGPLFYGAGGERTGSVSVHKFVAMWRKILQNCHDDAAKFVHLLMSPGCNYLVQEDFVPFLQDVVNTHPGLSFLKEASEFHSRYITTVIQRIFYAVNRSWSGRITCAELRRSSFLQNVALLEEEADINQLTEFFSYEHFYVIYCKFWELDTDHDLLIDADDLARHNDHALSTKMIDRIFSGAVTRGRKVQKEGKISYADFVWFLISEEDKKTPTSIEYWFRCMDLDGDGALSMFELEYFYEEQCRRLDSMAIEALPFQDCLCQMLDLVKPRTEGKITLQDLKRCKLANVFFDTFFNIEKYLDHEQKEQISLLRDGDSGGPELSDWEKYAAEEYDILVAEETAGEPWEDGDGVSPCCPGWSWMPELEGSPRLGPPTCRNYRCEPLPSLE

Target ORF information:

RefSeq Version	XM_011545630.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545630.2

ORF Insert Sequence:

ATGCCGCCCG GCAAAGTGCT GCAGCCGGTC CTGAAGATGA AGGTGGACGA GCTGTTCCTG 
TACTGGCTCA GCGAGGCCAG CACGCAGCGG ATGCTGCAGG ACTGCCTGCG CCGGATCAAG 
GCGCCCGGGC GGGACCAGCC GACCCCGGGG GACGGGGAGC AGCCCGGGGC CTGGCCCACA 
GCCCCGCTCG CCGCCCCCCG GCCCAGCGGG CTCGAACCCC CGGGAACCCC CGGGCCGGGC 
CCTGCGCTGC CCCTGGGCGC CGCCTCCAGC CCCAGGAACG CGCCCCACGT TCGAGGCACC 
CGTAGATCCG CAGGGACGAG AGTAGTTCAG ACACGGAAAG AAGAGCCTCT GCCCCCGGCC 
ACGAGCCAAA GCATTCCGAC CTTCTACTTC CCCAGAGGAC GCCCGCAGGA CTCCGTCAAC 
GTGGATGCCG TCATCAGCAA GATCGAGAGC ACCTTCGCCC GGTTCCCCCA CGAGAGGGCC 
ACCATGGATG ACATGGGCCT GGTGGCCAAG GCCTGCGGCT GCCCCCTCTA CTGGAAGGGG 
CCGCTCTTCT ATGGCGCCGG CGGGGAGCGC ACGGGCTCCG TGTCCGTCCA CAAGTTCGTC 
GCCATGTGGA GAAAAATCCT CCAGAACTGC CACGACGACG CGGCCAAGTT CGTCCATCTG 
CTCATGAGCC CCGGCTGCAA CTACCTGGTG CAGGAGGACT TTGTCCCCTT CTTGCAGGAC 
GTGGTGAACA CGCACCCGGG GCTGTCGTTC CTGAAGGAGG CGTCCGAGTT CCACTCGCGC 
TACATCACCA CGGTCATCCA GCGGATCTTC TACGCCGTGA ACCGGTCCTG GTCCGGCAGG 
ATCACCTGCG CCGAGCTGCG GAGGAGCTCC TTCCTGCAGA ATGTGGCGCT GCTGGAGGAG 
GAGGCGGACA TCAACCAGCT GACCGAATTC TTCTCGTACG AGCATTTCTA CGTCATCTAC 
TGCAAGTTCT GGGAGCTGGA CACGGACCAC GACCTGCTCA TCGACGCGGA CGACCTGGCG 
CGGCACAATG ACCACGCCCT TTCTACCAAG ATGATAGACA GGATCTTCTC AGGAGCAGTC 
ACACGAGGCA GAAAAGTGCA GAAGGAAGGG AAGATCAGCT ATGCCGACTT TGTCTGGTTT 
TTGATCTCTG AGGAAGACAA AAAAACACCG ACCAGCATCG AGTACTGGTT CCGCTGCATG 
GACCTGGACG GGGACGGCGC CCTGTCCATG TTCGAGCTCG AGTACTTCTA CGAGGAGCAG 
TGCCGAAGGC TGGACAGCAT GGCCATCGAG GCCCTGCCCT TCCAGGACTG CCTCTGCCAG 
ATGCTGGACC TGGTCAAGCC GAGGACTGAA GGGAAGATCA CGCTGCAGGA CCTGAAGCGC 
TGCAAGCTGG CTAACGTCTT CTTCGACACC TTCTTCAACA TCGAGAAGTA CCTCGACCAC 
GAGCAGAAAG AGCAGATCTC CCTGCTCAGG GACGGTGACA GCGGCGGCCC CGAGCTCTCG 
GACTGGGAGA AGTACGCGGC CGAGGAGTAC GACATCCTGG TGGCCGAGGA GACTGCGGGA 
GAGCCCTGGG AGGACGGAGA TGGGGTTTCA CCGTGTTGCC CAGGTTGGTC TTGGATGCCT 
GAGCTCGAGG GTTCTCCCCG CCTCGGTCCC CCAACGTGCC GGAATTACAG GTGTGAGCCC 
CTGCCCAGCC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP2R3B ( XM_011545630.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543932.1 Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), transcript variant X1, mRNA.

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CloneID	OHu67259
Clone ID Related Accession (Same CDS sequence)	XM_011546177.3 , XM_011545630.2 , XM_011545630.3 , XM_011546177.2
Accession Version	XM_011545630.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000024.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545630.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCCG GCAAAGTGCT GCAGCCGGTC CTGAAGATGA AGGTGGACGA GCTGTTCCTG 
TACTGGCTCA GCGAGGCCAG CACGCAGCGG ATGCTGCAGG ACTGCCTGCG CCGGATCAAG 
GCGCCCGGGC GGGACCAGCC GACCCCGGGG GACGGGGAGC AGCCCGGGGC CTGGCCCACA 
GCCCCGCTCG CCGCCCCCCG GCCCAGCGGG CTCGAACCCC CGGGAACCCC CGGGCCGGGC 
CCTGCGCTGC CCCTGGGCGC CGCCTCCAGC CCCAGGAACG CGCCCCACGT TCGAGGCACC 
CGTAGATCCG CAGGGACGAG AGTAGTTCAG ACACGGAAAG AAGAGCCTCT GCCCCCGGCC 
ACGAGCCAAA GCATTCCGAC CTTCTACTTC CCCAGAGGAC GCCCGCAGGA CTCCGTCAAC 
GTGGATGCCG TCATCAGCAA GATCGAGAGC ACCTTCGCCC GGTTCCCCCA CGAGAGGGCC 
ACCATGGATG ACATGGGCCT GGTGGCCAAG GCCTGCGGCT GCCCCCTCTA CTGGAAGGGG 
CCGCTCTTCT ATGGCGCCGG CGGGGAGCGC ACGGGCTCCG TGTCCGTCCA CAAGTTCGTC 
GCCATGTGGA GAAAAATCCT CCAGAACTGC CACGACGACG CGGCCAAGTT CGTCCATCTG 
CTCATGAGCC CCGGCTGCAA CTACCTGGTG CAGGAGGACT TTGTCCCCTT CTTGCAGGAC 
GTGGTGAACA CGCACCCGGG GCTGTCGTTC CTGAAGGAGG CGTCCGAGTT CCACTCGCGC 
TACATCACCA CGGTCATCCA GCGGATCTTC TACGCCGTGA ACCGGTCCTG GTCCGGCAGG 
ATCACCTGCG CCGAGCTGCG GAGGAGCTCC TTCCTGCAGA ATGTGGCGCT GCTGGAGGAG 
GAGGCGGACA TCAACCAGCT GACCGAATTC TTCTCGTACG AGCATTTCTA CGTCATCTAC 
TGCAAGTTCT GGGAGCTGGA CACGGACCAC GACCTGCTCA TCGACGCGGA CGACCTGGCG 
CGGCACAATG ACCACGCCCT TTCTACCAAG ATGATAGACA GGATCTTCTC AGGAGCAGTC 
ACACGAGGCA GAAAAGTGCA GAAGGAAGGG AAGATCAGCT ATGCCGACTT TGTCTGGTTT 
TTGATCTCTG AGGAAGACAA AAAAACACCG ACCAGCATCG AGTACTGGTT CCGCTGCATG 
GACCTGGACG GGGACGGCGC CCTGTCCATG TTCGAGCTCG AGTACTTCTA CGAGGAGCAG 
TGCCGAAGGC TGGACAGCAT GGCCATCGAG GCCCTGCCCT TCCAGGACTG CCTCTGCCAG 
ATGCTGGACC TGGTCAAGCC GAGGACTGAA GGGAAGATCA CGCTGCAGGA CCTGAAGCGC 
TGCAAGCTGG CTAACGTCTT CTTCGACACC TTCTTCAACA TCGAGAAGTA CCTCGACCAC 
GAGCAGAAAG AGCAGATCTC CCTGCTCAGG GACGGTGACA GCGGCGGCCC CGAGCTCTCG 
GACTGGGAGA AGTACGCGGC CGAGGAGTAC GACATCCTGG TGGCCGAGGA GACTGCGGGA 
GAGCCCTGGG AGGACGGAGA TGGGGTTTCA CCGTGTTGCC CAGGTTGGTC TTGGATGCCT 
GAGCTCGAGG GTTCTCCCCG CCTCGGTCCC CCAACGTGCC GGAATTACAG GTGTGAGCCC 
CTGCCCAGCC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543932.1
CDS	260..1957
Translation	MPPGKVLQPVLKMKVDELFLYWLSEASTQRMLQDCLRRIKAPGRDQPTPGDGEQPGAWPTAPLAAPRPSGLEPPGTPGPGPALPLGAASSPRNAPHVRGTRRSAGTRVVQTRKEEPLPPATSQSIPTFYFPRGRPQDSVNVDAVISKIESTFARFPHERATMDDMGLVAKACGCPLYWKGPLFYGAGGERTGSVSVHKFVAMWRKILQNCHDDAAKFVHLLMSPGCNYLVQEDFVPFLQDVVNTHPGLSFLKEASEFHSRYITTVIQRIFYAVNRSWSGRITCAELRRSSFLQNVALLEEEADINQLTEFFSYEHFYVIYCKFWELDTDHDLLIDADDLARHNDHALSTKMIDRIFSGAVTRGRKVQKEGKISYADFVWFLISEEDKKTPTSIEYWFRCMDLDGDGALSMFELEYFYEEQCRRLDSMAIEALPFQDCLCQMLDLVKPRTEGKITLQDLKRCKLANVFFDTFFNIEKYLDHEQKEQISLLRDGDSGGPELSDWEKYAAEEYDILVAEETAGEPWEDGDGVSPCCPGWSWMPELEGSPRLGPPTCRNYRCEPLPSLE

Target ORF information:

RefSeq Version	XM_011545630.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545630.3

ORF Insert Sequence:

ATGCCGCCCG GCAAAGTGCT GCAGCCGGTC CTGAAGATGA AGGTGGACGA GCTGTTCCTG 
TACTGGCTCA GCGAGGCCAG CACGCAGCGG ATGCTGCAGG ACTGCCTGCG CCGGATCAAG 
GCGCCCGGGC GGGACCAGCC GACCCCGGGG GACGGGGAGC AGCCCGGGGC CTGGCCCACA 
GCCCCGCTCG CCGCCCCCCG GCCCAGCGGG CTCGAACCCC CGGGAACCCC CGGGCCGGGC 
CCTGCGCTGC CCCTGGGCGC CGCCTCCAGC CCCAGGAACG CGCCCCACGT TCGAGGCACC 
CGTAGATCCG CAGGGACGAG AGTAGTTCAG ACACGGAAAG AAGAGCCTCT GCCCCCGGCC 
ACGAGCCAAA GCATTCCGAC CTTCTACTTC CCCAGAGGAC GCCCGCAGGA CTCCGTCAAC 
GTGGATGCCG TCATCAGCAA GATCGAGAGC ACCTTCGCCC GGTTCCCCCA CGAGAGGGCC 
ACCATGGATG ACATGGGCCT GGTGGCCAAG GCCTGCGGCT GCCCCCTCTA CTGGAAGGGG 
CCGCTCTTCT ATGGCGCCGG CGGGGAGCGC ACGGGCTCCG TGTCCGTCCA CAAGTTCGTC 
GCCATGTGGA GAAAAATCCT CCAGAACTGC CACGACGACG CGGCCAAGTT CGTCCATCTG 
CTCATGAGCC CCGGCTGCAA CTACCTGGTG CAGGAGGACT TTGTCCCCTT CTTGCAGGAC 
GTGGTGAACA CGCACCCGGG GCTGTCGTTC CTGAAGGAGG CGTCCGAGTT CCACTCGCGC 
TACATCACCA CGGTCATCCA GCGGATCTTC TACGCCGTGA ACCGGTCCTG GTCCGGCAGG 
ATCACCTGCG CCGAGCTGCG GAGGAGCTCC TTCCTGCAGA ATGTGGCGCT GCTGGAGGAG 
GAGGCGGACA TCAACCAGCT GACCGAATTC TTCTCGTACG AGCATTTCTA CGTCATCTAC 
TGCAAGTTCT GGGAGCTGGA CACGGACCAC GACCTGCTCA TCGACGCGGA CGACCTGGCG 
CGGCACAATG ACCACGCCCT TTCTACCAAG ATGATAGACA GGATCTTCTC AGGAGCAGTC 
ACACGAGGCA GAAAAGTGCA GAAGGAAGGG AAGATCAGCT ATGCCGACTT TGTCTGGTTT 
TTGATCTCTG AGGAAGACAA AAAAACACCG ACCAGCATCG AGTACTGGTT CCGCTGCATG 
GACCTGGACG GGGACGGCGC CCTGTCCATG TTCGAGCTCG AGTACTTCTA CGAGGAGCAG 
TGCCGAAGGC TGGACAGCAT GGCCATCGAG GCCCTGCCCT TCCAGGACTG CCTCTGCCAG 
ATGCTGGACC TGGTCAAGCC GAGGACTGAA GGGAAGATCA CGCTGCAGGA CCTGAAGCGC 
TGCAAGCTGG CTAACGTCTT CTTCGACACC TTCTTCAACA TCGAGAAGTA CCTCGACCAC 
GAGCAGAAAG AGCAGATCTC CCTGCTCAGG GACGGTGACA GCGGCGGCCC CGAGCTCTCG 
GACTGGGAGA AGTACGCGGC CGAGGAGTAC GACATCCTGG TGGCCGAGGA GACTGCGGGA 
GAGCCCTGGG AGGACGGAGA TGGGGTTTCA CCGTGTTGCC CAGGTTGGTC TTGGATGCCT 
GAGCTCGAGG GTTCTCCCCG CCTCGGTCCC CCAACGTGCC GGAATTACAG GTGTGAGCCC 
CTGCCCAGCC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP2R3B ( XM_011546177.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011544479.1 Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), transcript variant X1, mRNA.

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CloneID	OHu67259
Clone ID Related Accession (Same CDS sequence)	XM_011546177.3 , XM_011545630.2 , XM_011545630.3 , XM_011546177.2
Accession Version	XM_011546177.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011546177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCCG GCAAAGTGCT GCAGCCGGTC CTGAAGATGA AGGTGGACGA GCTGTTCCTG 
TACTGGCTCA GCGAGGCCAG CACGCAGCGG ATGCTGCAGG ACTGCCTGCG CCGGATCAAG 
GCGCCCGGGC GGGACCAGCC GACCCCGGGG GACGGGGAGC AGCCCGGGGC CTGGCCCACA 
GCCCCGCTCG CCGCCCCCCG GCCCAGCGGG CTCGAACCCC CGGGAACCCC CGGGCCGGGC 
CCTGCGCTGC CCCTGGGCGC CGCCTCCAGC CCCAGGAACG CGCCCCACGT TCGAGGCACC 
CGTAGATCCG CAGGGACGAG AGTAGTTCAG ACACGGAAAG AAGAGCCTCT GCCCCCGGCC 
ACGAGCCAAA GCATTCCGAC CTTCTACTTC CCCAGAGGAC GCCCGCAGGA CTCCGTCAAC 
GTGGATGCCG TCATCAGCAA GATCGAGAGC ACCTTCGCCC GGTTCCCCCA CGAGAGGGCC 
ACCATGGATG ACATGGGCCT GGTGGCCAAG GCCTGCGGCT GCCCCCTCTA CTGGAAGGGG 
CCGCTCTTCT ATGGCGCCGG CGGGGAGCGC ACGGGCTCCG TGTCCGTCCA CAAGTTCGTC 
GCCATGTGGA GAAAAATCCT CCAGAACTGC CACGACGACG CGGCCAAGTT CGTCCATCTG 
CTCATGAGCC CCGGCTGCAA CTACCTGGTG CAGGAGGACT TTGTCCCCTT CTTGCAGGAC 
GTGGTGAACA CGCACCCGGG GCTGTCGTTC CTGAAGGAGG CGTCCGAGTT CCACTCGCGC 
TACATCACCA CGGTCATCCA GCGGATCTTC TACGCCGTGA ACCGGTCCTG GTCCGGCAGG 
ATCACCTGCG CCGAGCTGCG GAGGAGCTCC TTCCTGCAGA ATGTGGCGCT GCTGGAGGAG 
GAGGCGGACA TCAACCAGCT GACCGAATTC TTCTCGTACG AGCATTTCTA CGTCATCTAC 
TGCAAGTTCT GGGAGCTGGA CACGGACCAC GACCTGCTCA TCGACGCGGA CGACCTGGCG 
CGGCACAATG ACCACGCCCT TTCTACCAAG ATGATAGACA GGATCTTCTC AGGAGCAGTC 
ACACGAGGCA GAAAAGTGCA GAAGGAAGGG AAGATCAGCT ATGCCGACTT TGTCTGGTTT 
TTGATCTCTG AGGAAGACAA AAAAACACCG ACCAGCATCG AGTACTGGTT CCGCTGCATG 
GACCTGGACG GGGACGGCGC CCTGTCCATG TTCGAGCTCG AGTACTTCTA CGAGGAGCAG 
TGCCGAAGGC TGGACAGCAT GGCCATCGAG GCCCTGCCCT TCCAGGACTG CCTCTGCCAG 
ATGCTGGACC TGGTCAAGCC GAGGACTGAA GGGAAGATCA CGCTGCAGGA CCTGAAGCGC 
TGCAAGCTGG CTAACGTCTT CTTCGACACC TTCTTCAACA TCGAGAAGTA CCTCGACCAC 
GAGCAGAAAG AGCAGATCTC CCTGCTCAGG GACGGTGACA GCGGCGGCCC CGAGCTCTCG 
GACTGGGAGA AGTACGCGGC CGAGGAGTAC GACATCCTGG TGGCCGAGGA GACTGCGGGA 
GAGCCCTGGG AGGACGGAGA TGGGGTTTCA CCGTGTTGCC CAGGTTGGTC TTGGATGCCT 
GAGCTCGAGG GTTCTCCCCG CCTCGGTCCC CCAACGTGCC GGAATTACAG GTGTGAGCCC 
CTGCCCAGCC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011544479.1
CDS	265..1962
Translation	MPPGKVLQPVLKMKVDELFLYWLSEASTQRMLQDCLRRIKAPGRDQPTPGDGEQPGAWPTAPLAAPRPSGLEPPGTPGPGPALPLGAASSPRNAPHVRGTRRSAGTRVVQTRKEEPLPPATSQSIPTFYFPRGRPQDSVNVDAVISKIESTFARFPHERATMDDMGLVAKACGCPLYWKGPLFYGAGGERTGSVSVHKFVAMWRKILQNCHDDAAKFVHLLMSPGCNYLVQEDFVPFLQDVVNTHPGLSFLKEASEFHSRYITTVIQRIFYAVNRSWSGRITCAELRRSSFLQNVALLEEEADINQLTEFFSYEHFYVIYCKFWELDTDHDLLIDADDLARHNDHALSTKMIDRIFSGAVTRGRKVQKEGKISYADFVWFLISEEDKKTPTSIEYWFRCMDLDGDGALSMFELEYFYEEQCRRLDSMAIEALPFQDCLCQMLDLVKPRTEGKITLQDLKRCKLANVFFDTFFNIEKYLDHEQKEQISLLRDGDSGGPELSDWEKYAAEEYDILVAEETAGEPWEDGDGVSPCCPGWSWMPELEGSPRLGPPTCRNYRCEPLPSLE

Target ORF information:

RefSeq Version	XM_011546177.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 2 regulatory subunit B''beta (PPP2R3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011546177.2

ORF Insert Sequence:

ATGCCGCCCG GCAAAGTGCT GCAGCCGGTC CTGAAGATGA AGGTGGACGA GCTGTTCCTG 
TACTGGCTCA GCGAGGCCAG CACGCAGCGG ATGCTGCAGG ACTGCCTGCG CCGGATCAAG 
GCGCCCGGGC GGGACCAGCC GACCCCGGGG GACGGGGAGC AGCCCGGGGC CTGGCCCACA 
GCCCCGCTCG CCGCCCCCCG GCCCAGCGGG CTCGAACCCC CGGGAACCCC CGGGCCGGGC 
CCTGCGCTGC CCCTGGGCGC CGCCTCCAGC CCCAGGAACG CGCCCCACGT TCGAGGCACC 
CGTAGATCCG CAGGGACGAG AGTAGTTCAG ACACGGAAAG AAGAGCCTCT GCCCCCGGCC 
ACGAGCCAAA GCATTCCGAC CTTCTACTTC CCCAGAGGAC GCCCGCAGGA CTCCGTCAAC 
GTGGATGCCG TCATCAGCAA GATCGAGAGC ACCTTCGCCC GGTTCCCCCA CGAGAGGGCC 
ACCATGGATG ACATGGGCCT GGTGGCCAAG GCCTGCGGCT GCCCCCTCTA CTGGAAGGGG 
CCGCTCTTCT ATGGCGCCGG CGGGGAGCGC ACGGGCTCCG TGTCCGTCCA CAAGTTCGTC 
GCCATGTGGA GAAAAATCCT CCAGAACTGC CACGACGACG CGGCCAAGTT CGTCCATCTG 
CTCATGAGCC CCGGCTGCAA CTACCTGGTG CAGGAGGACT TTGTCCCCTT CTTGCAGGAC 
GTGGTGAACA CGCACCCGGG GCTGTCGTTC CTGAAGGAGG CGTCCGAGTT CCACTCGCGC 
TACATCACCA CGGTCATCCA GCGGATCTTC TACGCCGTGA ACCGGTCCTG GTCCGGCAGG 
ATCACCTGCG CCGAGCTGCG GAGGAGCTCC TTCCTGCAGA ATGTGGCGCT GCTGGAGGAG 
GAGGCGGACA TCAACCAGCT GACCGAATTC TTCTCGTACG AGCATTTCTA CGTCATCTAC 
TGCAAGTTCT GGGAGCTGGA CACGGACCAC GACCTGCTCA TCGACGCGGA CGACCTGGCG 
CGGCACAATG ACCACGCCCT TTCTACCAAG ATGATAGACA GGATCTTCTC AGGAGCAGTC 
ACACGAGGCA GAAAAGTGCA GAAGGAAGGG AAGATCAGCT ATGCCGACTT TGTCTGGTTT 
TTGATCTCTG AGGAAGACAA AAAAACACCG ACCAGCATCG AGTACTGGTT CCGCTGCATG 
GACCTGGACG GGGACGGCGC CCTGTCCATG TTCGAGCTCG AGTACTTCTA CGAGGAGCAG 
TGCCGAAGGC TGGACAGCAT GGCCATCGAG GCCCTGCCCT TCCAGGACTG CCTCTGCCAG 
ATGCTGGACC TGGTCAAGCC GAGGACTGAA GGGAAGATCA CGCTGCAGGA CCTGAAGCGC 
TGCAAGCTGG CTAACGTCTT CTTCGACACC TTCTTCAACA TCGAGAAGTA CCTCGACCAC 
GAGCAGAAAG AGCAGATCTC CCTGCTCAGG GACGGTGACA GCGGCGGCCC CGAGCTCTCG 
GACTGGGAGA AGTACGCGGC CGAGGAGTAC GACATCCTGG TGGCCGAGGA GACTGCGGGA 
GAGCCCTGGG AGGACGGAGA TGGGGTTTCA CCGTGTTGCC CAGGTTGGTC TTGGATGCCT 
GAGCTCGAGG GTTCTCCCCG CCTCGGTCCC CCAACGTGCC GGAATTACAG GTGTGAGCCC 
CTGCCCAGCC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The protein phosphatase 2A regulatory subunit PR70 is a gonosomal melanoma tumor suppressor gene.
Science translational medicine8(369)369ra177(2016 Dec)
van Kempen LC,Redpath M,Elchebly M,Klein KO,Papadakis AI,Wilmott JS,Scolyer RA,Edqvist PH,Pont?n F,Schadendorf D,van Rijk AF,Michiels S,Dumay A,Helbling-Leclerc A,Dessen P,Wouters J,Stass M,Greenwood CM,Ghanem GE,van den Oord J,Feunteun J,Spatz A

Characterization and Genetic Analyses of New Genes Coding for NOD2 Interacting Proteins.
PloS one11(11)e0165420(2016)
Thi?baut R,Esmiol S,Lecine P,Mahfouz B,Hermant A,Nicoletti C,Parnis S,Perroy J,Borg JP,Pascoe L,Hugot JP,Ollendorff V

Structural and biochemical characterization of human PR70 in isolation and in complex with the scaffolding subunit of protein phosphatase 2A.
PloS one9(7)e101846(2014)
Dovega R,Tsutakawa S,Quistgaard EM,Anandapadamanaban M,L?w C,Nordlund P

Next-generation sequencing to generate interactome datasets.
Nature methods8(6)478-80(2011 Jun)
Yu H,Tardivo L,Tam S,Weiner E,Gebreab F,Fan C,Svrzikapa N,Hirozane-Kishikawa T,Rietman E,Yang X,Sahalie J,Salehi-Ashtiani K,Hao T,Cusick ME,Hill DE,Roth FP,Braun P,Vidal M

Identification and characterization of B"-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues.
European journal of biochemistry270(2)376-87(2003 Jan)
Stevens I,Janssens V,Martens E,Dilworth S,Goris J,Van Hoof C

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PPP2R3B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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