ABO cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ABO
Entrez Gene ID	28
Full Name	ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase
Synonyms	A3GALNT,A3GALT1,GTB,NAGAT
General protein information	Preferred Names ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase Names histo-blood group ABO system transferase ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase) B(A) alpha-1,3-galactosyltransferase histo-blood group A2 transferase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.2
Summary	This gene encodes proteins related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	110300
Disorder Html:	[Blood group, ABO system], 616093 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_020469.3	NP_065202.2	histo-blood group ABO system transferase
NM_020469.2	NP_065202.2	histo-blood group ABO system transferase

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Abo	NP_001153735.1
Rattus norvegicus (Norway rat)	Abo2	NP_001258227.1
Mus musculus (house mouse)	Abo	NP_109643.3
Homo sapiens (human)	ABO	NP_065202.2
	ABO	XP_001118424.2
Canis lupus familiaris (dog)	ABO	XP_005625199.1
Bos taurus (cattle)	ABO	NP_001071394.1
Pan troglodytes (chimpanzee)	ABO	NP_001181854.1
Rattus norvegicus (Norway rat)	Gbgt1	NP_075582.3

Gene Ontology
Bibliography

Related articles in PubMed

ABO transcript levels in peripheral blood and erythropoietic culture show different allele-related patterns independent of the CBF/NF-Y enhancer motif and multiple novel allele-specific variations in the 5'- and 3'-noncoding regions.
Thuresson B, Chester MA, Storry JR, Olsson ML
Transfusion48(3)493-504(2008 Mar)

Weak blood group B phenotypes may be caused by variations in the CCAAT-binding factor/NF-Y enhancer region of the ABO gene.
Seltsam A, Wagner FF, Gr?ger D, Gupta CD, Bade-Doeding C, Blasczyk R
Transfusion47(12)2330-5(2007 Dec)

Expression of the histo-blood group B gene predominates in AB-genotype cells.
Twu YC, Hsieh CY, Yu LC
Transfusion46(11)1988-96(2006 Nov)

ABO blood group determines plasma von Willebrand factor levels: a biologic function after all?
Jenkins PV, O'Donnell JS
Transfusion46(10)1836-44(2006 Oct)

Evolution of the O alleles of the human ABO blood group gene.
Roubinet F, Despiau S, Calafell F, Jin F, Bertranpetit J, Saitou N, Blancher A, Bertanpetit J
Transfusion44(5)707-15(2004 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

an ABO exonic missense mutation affects B-antigen expression by decreasing the mRNA level of the ABO gene
Title: An exonic missense mutation c.28G>A is associated with weak B blood group by affecting RNA splicing of the ABO gene.

Binding either of the donor substrate UDP-Gal or of UDP induces a semiclosed conformation of blood group glycosyltransferases A and B.
Title: Protein NMR Studies of Substrate Binding to Human Blood Group?A and B Glycosyltransferases.

Kinetic analysis of GTA and GTB point mutants E303C, E303D, E303Q and E303A shows that despite the enzymes having nearly identical sequences, the corresponding mutants of GTA/GTB have up to a 13-fold difference in their residual activities relative to wild type
Title: Glycosyltransfer in mutants of putative catalytic residue Glu303 of the human ABO(H) A and B blood group glycosyltransferases GTA and GTB proceeds through a labile active site.

These results support the contention that ABO expression is dependent upon a downstream positive regulatory element functioning through a tissue-restricted transcription factor, Elf5, in epithelial cells.
Title: Epithelial Expression of Human ABO Blood Group Genes Is Dependent upon a Downstream Regulatory Element Functioning through an Epithelial Cell-specific Transcription Factor, Elf5.

Our findings indicated that genetic variations of ABO gene may contribute to susceptibility of large artery atherosclerosis but not ischemic stroke and small vessel diseases in the Chinese population
Title: Association study of polymorphisms in the ABO gene with ischemic stroke in the Chinese population.

The following ABO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ABO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18243	NM_020469.3 Latest version!	Homo sapiens ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase (ABO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$144.50-$202.30 $289.00
View Old Accession Versions >>
OHu18243	NM_020469.2	Homo sapiens ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase (ABO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$144.50-$202.30 $289.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ABO ( NM_020469.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065202.2 Homo sapiens ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase (ABO), mRNA.

Price & Availability↑

CloneID	OHu18243
Clone ID Related Accession (Same CDS sequence)	NM_020469.3 , NM_020469.2
Accession Version	NM_020469.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-17
Organism	Homo sapiens(human)
Product	histo-blood group ABO system transferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV761252.1, AF134412.1, U15197.1 and AL772161.10. This sequence is a reference standard in the RefSeqGene project. On Sep 25, 2018 this sequence version replaced NM_020469.2. Sequence Note: This RefSeq record represents the ABO*A1.01 allele. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: JX519570.1, JX519569.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGCCGAGG TGTTGCGGAC GCTGGCCGGA AAACCAAAAT GCCACGCACT TCGACCTATG 
ATCCTTTTCC TAATAATGCT TGTCTTGGTC TTGTTTGGTT ACGGGGTCCT AAGCCCCAGA 
AGTCTAATGC CAGGAAGCCT GGAACGGGGG TTCTGCATGG CTGTTAGGGA ACCTGACCAT 
CTGCAGCGCG TCTCGTTGCC AAGGATGGTC TACCCCCAGC CAAAGGTGCT GACACCGTGT 
AGGAAGGATG TCCTCGTGGT GACCCCTTGG CTGGCTCCCA TTGTCTGGGA GGGCACATTC 
AACATCGACA TCCTCAACGA GCAGTTCAGG CTCCAGAACA CCACCATTGG GTTAACTGTG 
TTTGCCATCA AGAAATACGT GGCTTTCCTG AAGCTGTTCC TGGAGACGGC GGAGAAGCAC 
TTCATGGTGG GCCACCGTGT CCACTACTAT GTCTTCACCG ACCAGCCGGC CGCGGTGCCC 
CGCGTGACGC TGGGGACCGG TCGGCAGCTG TCAGTGCTGG AGGTGCGCGC CTACAAGCGC 
TGGCAGGACG TGTCCATGCG CCGCATGGAG ATGATCAGTG ACTTCTGCGA GCGGCGCTTC 
CTCAGCGAGG TGGATTACCT GGTGTGCGTG GACGTGGACA TGGAGTTCCG CGACCACGTG 
GGCGTGGAGA TCCTGACTCC GCTGTTCGGC ACCCTGCACC CCGGCTTCTA CGGAAGCAGC 
CGGGAGGCCT TCACCTACGA GCGCCGGCCC CAGTCCCAGG CCTACATCCC CAAGGACGAG 
GGCGATTTCT ACTACCTGGG GGGGTTCTTC GGGGGGTCGG TGCAAGAGGT GCAGCGGCTC 
ACCAGGGCCT GCCACCAGGC CATGATGGTC GACCAGGCCA ACGGCATCGA GGCCGTGTGG 
CACGACGAGA GCCACCTGAA CAAGTACCTG CTGCGCCACA AACCCACCAA GGTGCTCTCC 
CCCGAGTACT TGTGGGACCA GCAGCTGCTG GGCTGGCCCG CCGTCCTGAG GAAGCTGAGG 
TTCACTGCGG TGCCCAAGAA CCACCAGGCG GTCCGGAACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065202.2
CDS	13..1077
Translation	MAEVLRTLAGKPKCHALRPMILFLIMLVLVLFGYGVLSPRSLMPGSLERGFCMAVREPDHLQRVSLPRMVYPQPKVLTPCRKDVLVVTPWLAPIVWEGTFNIDILNEQFRLQNTTIGLTVFAIKKYVAFLKLFLETAEKHFMVGHRVHYYVFTDQPAAVPRVTLGTGRQLSVLEVRAYKRWQDVSMRRMEMISDFCERRFLSEVDYLVCVDVDMEFRDHVGVEILTPLFGTLHPGFYGSSREAFTYERRPQSQAYIPKDEGDFYYLGGFFGGSVQEVQRLTRACHQAMMVDQANGIEAVWHDESHLNKYLLRHKPTKVLSPEYLWDQQLLGWPAVLRKLRFTAVPKNHQAVRNP

Target ORF information:

RefSeq Version	NM_020469.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase (ABO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020469.3

ORF Insert Sequence:

ATGGCCGAGG TGTTGCGGAC GCTGGCCGGA AAACCAAAAT GCCACGCACT TCGACCTATG 
ATCCTTTTCC TAATAATGCT TGTCTTGGTC TTGTTTGGTT ACGGGGTCCT AAGCCCCAGA 
AGTCTAATGC CAGGAAGCCT GGAACGGGGG TTCTGCATGG CTGTTAGGGA ACCTGACCAT 
CTGCAGCGCG TCTCGTTGCC AAGGATGGTC TACCCCCAGC CAAAGGTGCT GACACCGTGT 
AGGAAGGATG TCCTCGTGGT GACCCCTTGG CTGGCTCCCA TTGTCTGGGA GGGCACATTC 
AACATCGACA TCCTCAACGA GCAGTTCAGG CTCCAGAACA CCACCATTGG GTTAACTGTG 
TTTGCCATCA AGAAATACGT GGCTTTCCTG AAGCTGTTCC TGGAGACGGC GGAGAAGCAC 
TTCATGGTGG GCCACCGTGT CCACTACTAT GTCTTCACCG ACCAGCCGGC CGCGGTGCCC 
CGCGTGACGC TGGGGACCGG TCGGCAGCTG TCAGTGCTGG AGGTGCGCGC CTACAAGCGC 
TGGCAGGACG TGTCCATGCG CCGCATGGAG ATGATCAGTG ACTTCTGCGA GCGGCGCTTC 
CTCAGCGAGG TGGATTACCT GGTGTGCGTG GACGTGGACA TGGAGTTCCG CGACCACGTG 
GGCGTGGAGA TCCTGACTCC GCTGTTCGGC ACCCTGCACC CCGGCTTCTA CGGAAGCAGC 
CGGGAGGCCT TCACCTACGA GCGCCGGCCC CAGTCCCAGG CCTACATCCC CAAGGACGAG 
GGCGATTTCT ACTACCTGGG GGGGTTCTTC GGGGGGTCGG TGCAAGAGGT GCAGCGGCTC 
ACCAGGGCCT GCCACCAGGC CATGATGGTC GACCAGGCCA ACGGCATCGA GGCCGTGTGG 
CACGACGAGA GCCACCTGAA CAAGTACCTG CTGCGCCACA AACCCACCAA GGTGCTCTCC 
CCCGAGTACT TGTGGGACCA GCAGCTGCTG GGCTGGCCCG CCGTCCTGAG GAAGCTGAGG 
TTCACTGCGG TGCCCAAGAA CCACCAGGCG GTCCGGAACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABO ( NM_020469.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065202.2 Homo sapiens ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase (ABO), mRNA.

Price & Availability↑

CloneID	OHu18243
Clone ID Related Accession (Same CDS sequence)	NM_020469.3 , NM_020469.2
Accession Version	NM_020469.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-28
Organism	Homo sapiens(human)
Product	histo-blood group ABO system transferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV761252.1, AF134412.1 and U15197.1. This sequence is a reference standard in the RefSeqGene project. On Jan 29, 2005 this sequence version replaced NM_020469.1. Sequence Note: This RefSeq record represents the ABO*A1.01.1.1 allele. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JX519569.1, JX519570.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGAGG TGTTGCGGAC GCTGGCCGGA AAACCAAAAT GCCACGCACT TCGACCTATG 
ATCCTTTTCC TAATAATGCT TGTCTTGGTC TTGTTTGGTT ACGGGGTCCT AAGCCCCAGA 
AGTCTAATGC CAGGAAGCCT GGAACGGGGG TTCTGCATGG CTGTTAGGGA ACCTGACCAT 
CTGCAGCGCG TCTCGTTGCC AAGGATGGTC TACCCCCAGC CAAAGGTGCT GACACCGTGT 
AGGAAGGATG TCCTCGTGGT GACCCCTTGG CTGGCTCCCA TTGTCTGGGA GGGCACATTC 
AACATCGACA TCCTCAACGA GCAGTTCAGG CTCCAGAACA CCACCATTGG GTTAACTGTG 
TTTGCCATCA AGAAATACGT GGCTTTCCTG AAGCTGTTCC TGGAGACGGC GGAGAAGCAC 
TTCATGGTGG GCCACCGTGT CCACTACTAT GTCTTCACCG ACCAGCCGGC CGCGGTGCCC 
CGCGTGACGC TGGGGACCGG TCGGCAGCTG TCAGTGCTGG AGGTGCGCGC CTACAAGCGC 
TGGCAGGACG TGTCCATGCG CCGCATGGAG ATGATCAGTG ACTTCTGCGA GCGGCGCTTC 
CTCAGCGAGG TGGATTACCT GGTGTGCGTG GACGTGGACA TGGAGTTCCG CGACCACGTG 
GGCGTGGAGA TCCTGACTCC GCTGTTCGGC ACCCTGCACC CCGGCTTCTA CGGAAGCAGC 
CGGGAGGCCT TCACCTACGA GCGCCGGCCC CAGTCCCAGG CCTACATCCC CAAGGACGAG 
GGCGATTTCT ACTACCTGGG GGGGTTCTTC GGGGGGTCGG TGCAAGAGGT GCAGCGGCTC 
ACCAGGGCCT GCCACCAGGC CATGATGGTC GACCAGGCCA ACGGCATCGA GGCCGTGTGG 
CACGACGAGA GCCACCTGAA CAAGTACCTG CTGCGCCACA AACCCACCAA GGTGCTCTCC 
CCCGAGTACT TGTGGGACCA GCAGCTGCTG GGCTGGCCCG CCGTCCTGAG GAAGCTGAGG 
TTCACTGCGG TGCCCAAGAA CCACCAGGCG GTCCGGAACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065202.2
CDS	26..1090
Translation	MAEVLRTLAGKPKCHALRPMILFLIMLVLVLFGYGVLSPRSLMPGSLERGFCMAVREPDHLQRVSLPRMVYPQPKVLTPCRKDVLVVTPWLAPIVWEGTFNIDILNEQFRLQNTTIGLTVFAIKKYVAFLKLFLETAEKHFMVGHRVHYYVFTDQPAAVPRVTLGTGRQLSVLEVRAYKRWQDVSMRRMEMISDFCERRFLSEVDYLVCVDVDMEFRDHVGVEILTPLFGTLHPGFYGSSREAFTYERRPQSQAYIPKDEGDFYYLGGFFGGSVQEVQRLTRACHQAMMVDQANGIEAVWHDESHLNKYLLRHKPTKVLSPEYLWDQQLLGWPAVLRKLRFTAVPKNHQAVRNP

Target ORF information:

RefSeq Version	NM_020469.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase (ABO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020469.2

ORF Insert Sequence:

ATGGCCGAGG TGTTGCGGAC GCTGGCCGGA AAACCAAAAT GCCACGCACT TCGACCTATG 
ATCCTTTTCC TAATAATGCT TGTCTTGGTC TTGTTTGGTT ACGGGGTCCT AAGCCCCAGA 
AGTCTAATGC CAGGAAGCCT GGAACGGGGG TTCTGCATGG CTGTTAGGGA ACCTGACCAT 
CTGCAGCGCG TCTCGTTGCC AAGGATGGTC TACCCCCAGC CAAAGGTGCT GACACCGTGT 
AGGAAGGATG TCCTCGTGGT GACCCCTTGG CTGGCTCCCA TTGTCTGGGA GGGCACATTC 
AACATCGACA TCCTCAACGA GCAGTTCAGG CTCCAGAACA CCACCATTGG GTTAACTGTG 
TTTGCCATCA AGAAATACGT GGCTTTCCTG AAGCTGTTCC TGGAGACGGC GGAGAAGCAC 
TTCATGGTGG GCCACCGTGT CCACTACTAT GTCTTCACCG ACCAGCCGGC CGCGGTGCCC 
CGCGTGACGC TGGGGACCGG TCGGCAGCTG TCAGTGCTGG AGGTGCGCGC CTACAAGCGC 
TGGCAGGACG TGTCCATGCG CCGCATGGAG ATGATCAGTG ACTTCTGCGA GCGGCGCTTC 
CTCAGCGAGG TGGATTACCT GGTGTGCGTG GACGTGGACA TGGAGTTCCG CGACCACGTG 
GGCGTGGAGA TCCTGACTCC GCTGTTCGGC ACCCTGCACC CCGGCTTCTA CGGAAGCAGC 
CGGGAGGCCT TCACCTACGA GCGCCGGCCC CAGTCCCAGG CCTACATCCC CAAGGACGAG 
GGCGATTTCT ACTACCTGGG GGGGTTCTTC GGGGGGTCGG TGCAAGAGGT GCAGCGGCTC 
ACCAGGGCCT GCCACCAGGC CATGATGGTC GACCAGGCCA ACGGCATCGA GGCCGTGTGG 
CACGACGAGA GCCACCTGAA CAAGTACCTG CTGCGCCACA AACCCACCAA GGTGCTCTCC 
CCCGAGTACT TGTGGGACCA GCAGCTGCTG GGCTGGCCCG CCGTCCTGAG GAAGCTGAGG 
TTCACTGCGG TGCCCAAGAA CCACCAGGCG GTCCGGAACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ABO transcript levels in peripheral blood and erythropoietic culture show different allele-related patterns independent of the CBF/NF-Y enhancer motif and multiple novel allele-specific variations in the 5'- and 3'-noncoding regions.
Transfusion48(3)493-504(2008 Mar)
Thuresson B,Chester MA,Storry JR,Olsson ML

Weak blood group B phenotypes may be caused by variations in the CCAAT-binding factor/NF-Y enhancer region of the ABO gene.
Transfusion47(12)2330-5(2007 Dec)
Seltsam A,Wagner FF,Gr?ger D,Gupta CD,Bade-Doeding C,Blasczyk R

Expression of the histo-blood group B gene predominates in AB-genotype cells.
Transfusion46(11)1988-96(2006 Nov)
Twu YC,Hsieh CY,Yu LC

ABO blood group determines plasma von Willebrand factor levels: a biologic function after all?
Transfusion46(10)1836-44(2006 Oct)
Jenkins PV,O'Donnell JS

Evolution of the O alleles of the human ABO blood group gene.
Transfusion44(5)707-15(2004 May)
Roubinet F,Despiau S,Calafell F,Jin F,Bertranpetit J,Saitou N,Blancher A,Bertanpetit J

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ABO cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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