GNS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GNS
Entrez Gene ID	2799
Full Name	glucosamine (N-acetyl)-6-sulfatase
Synonyms	G6S
General protein information	Preferred Names glucosamine (N-acetyl)-6-sulfatase Names N-acetylglucosamine-6-sulfatase glucosamine -6-sulfatase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q14.3
Summary	The product of this gene is a lysosomal enzyme found in all cells. It is involved in the catabolism of heparin, heparan sulphate, and keratan sulphate. Deficiency of this enzyme results in the accumulation of undegraded substrate and the lysosomal storage disorder mucopolysaccharidosis type IIID (Sanfilippo D syndrome). Mucopolysaccharidosis type IIID is the least common of the four subtypes of Sanfilippo syndrome. [provided by RefSeq, Jul 2008].
Disorder MIM:	607664
Disorder Html:	Mucopolysaccharidosis type IIID, 252940 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002076.3	NP_002067.1	N-acetylglucosamine-6-sulfatase precursor
NM_002076.4	NP_002067.1	N-acetylglucosamine-6-sulfatase precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gns	NP_001011989.1
Rattus norvegicus (Norway rat)	LOC100909505	XP_003750373.1
Canis lupus familiaris (dog)	GNS	XP_531660.4
Drosophila melanogaster (fruit fly)	CG18278	NP_725289.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004721	XP_318108.3
Magnaporthe oryzae (rice blast fungus)	MGG_07920	XP_003713163.1
Xenopus tropicalis (tropical clawed frog)	gns	NP_001107314.1
Homo sapiens (human)	GNS	NP_002067.1
Bos taurus (cattle)	GNS	NP_001069030.1
Magnaporthe oryzae (rice blast fungus)	MGG_15457	XP_003710411.1
Neurospora crassa	NCU06041	XP_959804.2
	GNS	XP_001116940.2
Gallus gallus (chicken)	GNS	NP_001186488.1
Drosophila melanogaster (fruit fly)	CG30059	NP_610872.1
Pan troglodytes (chimpanzee)	GNS	XP_522454.1
Mus musculus (house mouse)	Gns	NP_083640.1
Danio rerio (zebrafish)	gnsa	NP_001025379.1

Gene Ontology
Bibliography

Related articles in PubMed

Disease correction by AAV-mediated gene therapy in a new mouse model of mucopolysaccharidosis type IIID.
Roca C, Motas S, Marc? S, Ribera A, S?nchez V, S?nchez X, Bertolin J, Le?n X, P?rez J, Garcia M, Villacampa P, Ruberte J, Pujol A, Haurigot V, Bosch F
Human molecular genetics26(8)1535-1551(2017 04)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Mucopolysaccharidosis type IIID: 12 new patients and 15 novel mutations.
Valstar MJ, Bertoli-Avella AM, Wessels MW, Ruijter GJ, de Graaf B, Olmer R, Elfferich P, Neijs S, Kariminejad R, Suheyl Ezg? F, Tokatli A, Czartoryska B, Bosschaart AN, van den Bos-Terpstra F, Puissant H, B?rger F, Omran H, Eckert D, Filocamo M, Simeonov E, Willems PJ, Wevers RA, Niermeijer MF, Halley DJ, Poorthuis BJ, van Diggelen OP
Human mutation31(5)E1348-60(2010 May)

A novel loss-of-function mutation in the GNS gene causes Sanfilippo syndrome type D.
El?ioglu NH, Pawlik B, Colak B, Beck M, Wollnik B
Genetic counseling (Geneva, Switzerland)20(2)133-9(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mice deficient in GNS showed lysosomal storage pathology and a phenotype that closely resembled human MPSIIID. Moreover, treatment of the GNS-deficient animals with GNS-encoding adeno-associated viral (AAV) vectors of serotype 9 delivered to the cerebrospinal fluid completely corrected pathological storage, improved lysosomal functionality in the CNS and somatic tissues, resolved neuroinflammation
Title: Disease correction by AAV-mediated gene therapy in a new mouse model of mucopolysaccharidosis type IIID.

12 new patients and 15 novel mutations were identified in Mucopolysaccharidosis type IIID.
Title: Mucopolysaccharidosis type IIID: 12 new patients and 15 novel mutations.

We identified the novel homozygous single base pair insertion, c.1226GinsG, which leads to a frame-shift and a premature truncation of the GNS protein (p.R409Rfs21X).
Title: A novel loss-of-function mutation in the GNS gene causes Sanfilippo syndrome type D.

Sanfilippo syndrome type D has 3 novel mutations in the GNS Gene.
Title: Sanfilippo syndrome type D: natural history and identification of 3 novel mutations in the GNS Gene.

A large intragenic deletion of 8723 bp encompassing exons 2 and 3 has been identified, the first large intragenic deletion to be reported in any of the four Sanfilippo subtypes. Q272X has also been found.
Title: Identification and characterisation of an 8.7 kb deletion and a novel nonsense mutation in two Italian families with Sanfilippo syndrome type D (mucopolysaccharidosis IIID).

The following GNS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19259	NM_002076.4 Latest version!	Homo sapiens glucosamine (N-acetyl)-6-sulfatase (GNS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
View Old Accession Versions >>
OHu19259	NM_002076.3	Homo sapiens glucosamine (N-acetyl)-6-sulfatase (GNS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNS ( NM_002076.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002067.1 Homo sapiens glucosamine (N-acetyl)-6-sulfatase (GNS), mRNA.

Price & Availability↑

CloneID	OHu19259
Clone ID Related Accession (Same CDS sequence)	NM_002076.3 , NM_002076.4
Accession Version	NM_002076.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	N-acetylglucosamine-6-sulfatase precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA437901.1, DA333542.1, BC012482.1, BX537363.1 and AA725727.1. This sequence is a reference standard in the RefSeqGene project. On May 29, 2008 this sequence version replaced NM_002076.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.146955.1, SRR1803617.19618.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCTCC TGCCTCTAGC CCCAGGTCGG CTCCGGCGGG GCAGCCCCCG CCACCTGCCC 
TCCTGCAGCC CAGCGCTGCT ACTGCTGGTG CTGGGCGGCT GCCTGGGGGT CTTCGGGGTG 
GCTGCGGGAA CCCGGAGGCC CAACGTGGTG CTGCTCCTCA CGGACGACCA GGACGAAGTG 
CTCGGCGGCA TGACACCGCT AAAGAAAACC AAAGCTCTCA TCGGAGAGAT GGGGATGACT 
TTTTCCAGTG CTTATGTGCC AAGTGCTCTC TGCTGCCCCA GCAGAGCCAG TATCCTGACA 
GGAAAGTACC CACATAATCA TCACGTTGTG AACAACACTC TGGAGGGGAA CTGCAGTAGT 
AAGTCCTGGC AGAAGATCCA AGAACCAAAT ACTTTCCCAG CAATTCTCAG ATCAATGTGT 
GGTTATCAGA CCTTTTTTGC AGGGAAATAT TTAAATGAGT ACGGAGCCCC AGATGCAGGT 
GGACTAGAAC ACGTTCCTCT GGGTTGGAGT TACTGGTATG CCTTGGAAAA GAATTCTAAG 
TATTATAATT ACACCCTGTC TATCAATGGG AAGGCACGGA AGCATGGTGA AAACTATAGT 
GTGGACTACC TGACAGATGT TTTGGCTAAT GTCTCCTTGG ACTTTCTGGA CTACAAGTCC 
AACTTTGAGC CCTTCTTCAT GATGATCGCC ACTCCAGCGC CTCATTCGCC TTGGACAGCT 
GCACCTCAGT ACCAGAAGGC TTTCCAGAAT GTCTTTGCAC CAAGAAACAA GAACTTCAAC 
ATCCATGGAA CGAACAAGCA CTGGTTAATT AGGCAAGCCA AGACTCCAAT GACTAATTCT 
TCAATACAGT TTTTAGATAA TGCATTTAGG AAAAGGTGGC AAACTCTCCT CTCAGTTGAT 
GACCTTGTGG AGAAACTGGT CAAGAGGCTG GAGTTCACTG GGGAGCTCAA CAACACTTAC 
ATCTTCTATA CCTCAGACAA TGGCTATCAC ACAGGACAGT TTTCCTTGCC AATAGACAAG 
AGACAGCTGT ATGAGTTTGA TATCAAAGTT CCACTGTTGG TTCGAGGACC TGGGATCAAA 
CCAAATCAGA CAAGCAAGAT GCTGGTTGCC AACATTGACT TGGGTCCTAC TATTTTGGAC 
ATTGCTGGCT ACGACCTAAA TAAGACACAG ATGGATGGGA TGTCCTTATT GCCCATTTTG 
AGAGGTGCCA GTAACTTGAC CTGGCGATCA GATGTCCTGG TGGAATACCA AGGAGAAGGC 
CGTAACGTCA CTGACCCAAC ATGCCCTTCC CTGAGTCCTG GCGTATCTCA ATGCTTCCCA 
GACTGTGTAT GTGAAGATGC TTATAACAAT ACCTATGCCT GTGTGAGGAC AATGTCAGCA 
TTGTGGAATT TGCAGTATTG CGAGTTTGAT GACCAGGAGG TGTTTGTAGA AGTCTATAAT 
CTGACTGCAG ACCCAGACCA GATCACTAAC ATTGCTAAAA CCATAGACCC AGAGCTTTTA 
GGAAAGATGA ACTATCGGTT AATGATGTTA CAGTCCTGTT CTGGGCCAAC CTGTCGCACT 
CCAGGGGTTT TTGACCCCGG ATACAGGTTT GACCCCCGTC TCATGTTCAG CAATCGCGGC 
AGTGTCAGGA CTCGAAGATT TTCCAAACAT CTTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002067.1
CDS	171..1829
Translation	MRLLPLAPGRLRRGSPRHLPSCSPALLLLVLGGCLGVFGVAAGTRRPNVVLLLTDDQDEVLGGMTPLKKTKALIGEMGMTFSSAYVPSALCCPSRASILTGKYPHNHHVVNNTLEGNCSSKSWQKIQEPNTFPAILRSMCGYQTFFAGKYLNEYGAPDAGGLEHVPLGWSYWYALEKNSKYYNYTLSINGKARKHGENYSVDYLTDVLANVSLDFLDYKSNFEPFFMMIATPAPHSPWTAAPQYQKAFQNVFAPRNKNFNIHGTNKHWLIRQAKTPMTNSSIQFLDNAFRKRWQTLLSVDDLVEKLVKRLEFTGELNNTYIFYTSDNGYHTGQFSLPIDKRQLYEFDIKVPLLVRGPGIKPNQTSKMLVANIDLGPTILDIAGYDLNKTQMDGMSLLPILRGASNLTWRSDVLVEYQGEGRNVTDPTCPSLSPGVSQCFPDCVCEDAYNNTYACVRTMSALWNLQYCEFDDQEVFVEVYNLTADPDQITNIAKTIDPELLGKMNYRLMMLQSCSGPTCRTPGVFDPGYRFDPRLMFSNRGSVRTRRFSKHLL

Target ORF information:

RefSeq Version	NM_002076.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (N-acetyl)-6-sulfatase (GNS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002076.3

ORF Insert Sequence:

ATGCGGCTCC TGCCTCTAGC CCCAGGTCGG CTCCGGCGGG GCAGCCCCCG CCACCTGCCC 
TCCTGCAGCC CAGCGCTGCT ACTGCTGGTG CTGGGCGGCT GCCTGGGGGT CTTCGGGGTG 
GCTGCGGGAA CCCGGAGGCC CAACGTGGTG CTGCTCCTCA CGGACGACCA GGACGAAGTG 
CTCGGCGGCA TGACACCGCT AAAGAAAACC AAAGCTCTCA TCGGAGAGAT GGGGATGACT 
TTTTCCAGTG CTTATGTGCC AAGTGCTCTC TGCTGCCCCA GCAGAGCCAG TATCCTGACA 
GGAAAGTACC CACATAATCA TCACGTTGTG AACAACACTC TGGAGGGGAA CTGCAGTAGT 
AAGTCCTGGC AGAAGATCCA AGAACCAAAT ACTTTCCCAG CAATTCTCAG ATCAATGTGT 
GGTTATCAGA CCTTTTTTGC AGGGAAATAT TTAAATGAGT ACGGAGCCCC AGATGCAGGT 
GGACTAGAAC ACGTTCCTCT GGGTTGGAGT TACTGGTATG CCTTGGAAAA GAATTCTAAG 
TATTATAATT ACACCCTGTC TATCAATGGG AAGGCACGGA AGCATGGTGA AAACTATAGT 
GTGGACTACC TGACAGATGT TTTGGCTAAT GTCTCCTTGG ACTTTCTGGA CTACAAGTCC 
AACTTTGAGC CCTTCTTCAT GATGATCGCC ACTCCAGCGC CTCATTCGCC TTGGACAGCT 
GCACCTCAGT ACCAGAAGGC TTTCCAGAAT GTCTTTGCAC CAAGAAACAA GAACTTCAAC 
ATCCATGGAA CGAACAAGCA CTGGTTAATT AGGCAAGCCA AGACTCCAAT GACTAATTCT 
TCAATACAGT TTTTAGATAA TGCATTTAGG AAAAGGTGGC AAACTCTCCT CTCAGTTGAT 
GACCTTGTGG AGAAACTGGT CAAGAGGCTG GAGTTCACTG GGGAGCTCAA CAACACTTAC 
ATCTTCTATA CCTCAGACAA TGGCTATCAC ACAGGACAGT TTTCCTTGCC AATAGACAAG 
AGACAGCTGT ATGAGTTTGA TATCAAAGTT CCACTGTTGG TTCGAGGACC TGGGATCAAA 
CCAAATCAGA CAAGCAAGAT GCTGGTTGCC AACATTGACT TGGGTCCTAC TATTTTGGAC 
ATTGCTGGCT ACGACCTAAA TAAGACACAG ATGGATGGGA TGTCCTTATT GCCCATTTTG 
AGAGGTGCCA GTAACTTGAC CTGGCGATCA GATGTCCTGG TGGAATACCA AGGAGAAGGC 
CGTAACGTCA CTGACCCAAC ATGCCCTTCC CTGAGTCCTG GCGTATCTCA ATGCTTCCCA 
GACTGTGTAT GTGAAGATGC TTATAACAAT ACCTATGCCT GTGTGAGGAC AATGTCAGCA 
TTGTGGAATT TGCAGTATTG CGAGTTTGAT GACCAGGAGG TGTTTGTAGA AGTCTATAAT 
CTGACTGCAG ACCCAGACCA GATCACTAAC ATTGCTAAAA CCATAGACCC AGAGCTTTTA 
GGAAAGATGA ACTATCGGTT AATGATGTTA CAGTCCTGTT CTGGGCCAAC CTGTCGCACT 
CCAGGGGTTT TTGACCCCGG ATACAGGTTT GACCCCCGTC TCATGTTCAG CAATCGCGGC 
AGTGTCAGGA CTCGAAGATT TTCCAAACAT CTTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNS ( NM_002076.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002067.1 Homo sapiens glucosamine (N-acetyl)-6-sulfatase (GNS), mRNA.

Price & Availability↑

CloneID	OHu19259
Clone ID Related Accession (Same CDS sequence)	NM_002076.3 , NM_002076.4
Accession Version	NM_002076.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	N-acetylglucosamine-6-sulfatase precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA333542.1, BC012482.1 and BX537363.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_002076.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.55744.1, SRR1803616.138171.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000258145.8/ ENSP00000258145.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGCTCC TGCCTCTAGC CCCAGGTCGG CTCCGGCGGG GCAGCCCCCG CCACCTGCCC 
TCCTGCAGCC CAGCGCTGCT ACTGCTGGTG CTGGGCGGCT GCCTGGGGGT CTTCGGGGTG 
GCTGCGGGAA CCCGGAGGCC CAACGTGGTG CTGCTCCTCA CGGACGACCA GGACGAAGTG 
CTCGGCGGCA TGACACCGCT AAAGAAAACC AAAGCTCTCA TCGGAGAGAT GGGGATGACT 
TTTTCCAGTG CTTATGTGCC AAGTGCTCTC TGCTGCCCCA GCAGAGCCAG TATCCTGACA 
GGAAAGTACC CACATAATCA TCACGTTGTG AACAACACTC TGGAGGGGAA CTGCAGTAGT 
AAGTCCTGGC AGAAGATCCA AGAACCAAAT ACTTTCCCAG CAATTCTCAG ATCAATGTGT 
GGTTATCAGA CCTTTTTTGC AGGGAAATAT TTAAATGAGT ACGGAGCCCC AGATGCAGGT 
GGACTAGAAC ACGTTCCTCT GGGTTGGAGT TACTGGTATG CCTTGGAAAA GAATTCTAAG 
TATTATAATT ACACCCTGTC TATCAATGGG AAGGCACGGA AGCATGGTGA AAACTATAGT 
GTGGACTACC TGACAGATGT TTTGGCTAAT GTCTCCTTGG ACTTTCTGGA CTACAAGTCC 
AACTTTGAGC CCTTCTTCAT GATGATCGCC ACTCCAGCGC CTCATTCGCC TTGGACAGCT 
GCACCTCAGT ACCAGAAGGC TTTCCAGAAT GTCTTTGCAC CAAGAAACAA GAACTTCAAC 
ATCCATGGAA CGAACAAGCA CTGGTTAATT AGGCAAGCCA AGACTCCAAT GACTAATTCT 
TCAATACAGT TTTTAGATAA TGCATTTAGG AAAAGGTGGC AAACTCTCCT CTCAGTTGAT 
GACCTTGTGG AGAAACTGGT CAAGAGGCTG GAGTTCACTG GGGAGCTCAA CAACACTTAC 
ATCTTCTATA CCTCAGACAA TGGCTATCAC ACAGGACAGT TTTCCTTGCC AATAGACAAG 
AGACAGCTGT ATGAGTTTGA TATCAAAGTT CCACTGTTGG TTCGAGGACC TGGGATCAAA 
CCAAATCAGA CAAGCAAGAT GCTGGTTGCC AACATTGACT TGGGTCCTAC TATTTTGGAC 
ATTGCTGGCT ACGACCTAAA TAAGACACAG ATGGATGGGA TGTCCTTATT GCCCATTTTG 
AGAGGTGCCA GTAACTTGAC CTGGCGATCA GATGTCCTGG TGGAATACCA AGGAGAAGGC 
CGTAACGTCA CTGACCCAAC ATGCCCTTCC CTGAGTCCTG GCGTATCTCA ATGCTTCCCA 
GACTGTGTAT GTGAAGATGC TTATAACAAT ACCTATGCCT GTGTGAGGAC AATGTCAGCA 
TTGTGGAATT TGCAGTATTG CGAGTTTGAT GACCAGGAGG TGTTTGTAGA AGTCTATAAT 
CTGACTGCAG ACCCAGACCA GATCACTAAC ATTGCTAAAA CCATAGACCC AGAGCTTTTA 
GGAAAGATGA ACTATCGGTT AATGATGTTA CAGTCCTGTT CTGGGCCAAC CTGTCGCACT 
CCAGGGGTTT TTGACCCCGG ATACAGGTTT GACCCCCGTC TCATGTTCAG CAATCGCGGC 
AGTGTCAGGA CTCGAAGATT TTCCAAACAT CTTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002067.1
CDS	131..1789
Translation	MRLLPLAPGRLRRGSPRHLPSCSPALLLLVLGGCLGVFGVAAGTRRPNVVLLLTDDQDEVLGGMTPLKKTKALIGEMGMTFSSAYVPSALCCPSRASILTGKYPHNHHVVNNTLEGNCSSKSWQKIQEPNTFPAILRSMCGYQTFFAGKYLNEYGAPDAGGLEHVPLGWSYWYALEKNSKYYNYTLSINGKARKHGENYSVDYLTDVLANVSLDFLDYKSNFEPFFMMIATPAPHSPWTAAPQYQKAFQNVFAPRNKNFNIHGTNKHWLIRQAKTPMTNSSIQFLDNAFRKRWQTLLSVDDLVEKLVKRLEFTGELNNTYIFYTSDNGYHTGQFSLPIDKRQLYEFDIKVPLLVRGPGIKPNQTSKMLVANIDLGPTILDIAGYDLNKTQMDGMSLLPILRGASNLTWRSDVLVEYQGEGRNVTDPTCPSLSPGVSQCFPDCVCEDAYNNTYACVRTMSALWNLQYCEFDDQEVFVEVYNLTADPDQITNIAKTIDPELLGKMNYRLMMLQSCSGPTCRTPGVFDPGYRFDPRLMFSNRGSVRTRRFSKHLL

Target ORF information:

RefSeq Version	NM_002076.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (N-acetyl)-6-sulfatase (GNS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002076.4

ORF Insert Sequence:

ATGCGGCTCC TGCCTCTAGC CCCAGGTCGG CTCCGGCGGG GCAGCCCCCG CCACCTGCCC 
TCCTGCAGCC CAGCGCTGCT ACTGCTGGTG CTGGGCGGCT GCCTGGGGGT CTTCGGGGTG 
GCTGCGGGAA CCCGGAGGCC CAACGTGGTG CTGCTCCTCA CGGACGACCA GGACGAAGTG 
CTCGGCGGCA TGACACCGCT AAAGAAAACC AAAGCTCTCA TCGGAGAGAT GGGGATGACT 
TTTTCCAGTG CTTATGTGCC AAGTGCTCTC TGCTGCCCCA GCAGAGCCAG TATCCTGACA 
GGAAAGTACC CACATAATCA TCACGTTGTG AACAACACTC TGGAGGGGAA CTGCAGTAGT 
AAGTCCTGGC AGAAGATCCA AGAACCAAAT ACTTTCCCAG CAATTCTCAG ATCAATGTGT 
GGTTATCAGA CCTTTTTTGC AGGGAAATAT TTAAATGAGT ACGGAGCCCC AGATGCAGGT 
GGACTAGAAC ACGTTCCTCT GGGTTGGAGT TACTGGTATG CCTTGGAAAA GAATTCTAAG 
TATTATAATT ACACCCTGTC TATCAATGGG AAGGCACGGA AGCATGGTGA AAACTATAGT 
GTGGACTACC TGACAGATGT TTTGGCTAAT GTCTCCTTGG ACTTTCTGGA CTACAAGTCC 
AACTTTGAGC CCTTCTTCAT GATGATCGCC ACTCCAGCGC CTCATTCGCC TTGGACAGCT 
GCACCTCAGT ACCAGAAGGC TTTCCAGAAT GTCTTTGCAC CAAGAAACAA GAACTTCAAC 
ATCCATGGAA CGAACAAGCA CTGGTTAATT AGGCAAGCCA AGACTCCAAT GACTAATTCT 
TCAATACAGT TTTTAGATAA TGCATTTAGG AAAAGGTGGC AAACTCTCCT CTCAGTTGAT 
GACCTTGTGG AGAAACTGGT CAAGAGGCTG GAGTTCACTG GGGAGCTCAA CAACACTTAC 
ATCTTCTATA CCTCAGACAA TGGCTATCAC ACAGGACAGT TTTCCTTGCC AATAGACAAG 
AGACAGCTGT ATGAGTTTGA TATCAAAGTT CCACTGTTGG TTCGAGGACC TGGGATCAAA 
CCAAATCAGA CAAGCAAGAT GCTGGTTGCC AACATTGACT TGGGTCCTAC TATTTTGGAC 
ATTGCTGGCT ACGACCTAAA TAAGACACAG ATGGATGGGA TGTCCTTATT GCCCATTTTG 
AGAGGTGCCA GTAACTTGAC CTGGCGATCA GATGTCCTGG TGGAATACCA AGGAGAAGGC 
CGTAACGTCA CTGACCCAAC ATGCCCTTCC CTGAGTCCTG GCGTATCTCA ATGCTTCCCA 
GACTGTGTAT GTGAAGATGC TTATAACAAT ACCTATGCCT GTGTGAGGAC AATGTCAGCA 
TTGTGGAATT TGCAGTATTG CGAGTTTGAT GACCAGGAGG TGTTTGTAGA AGTCTATAAT 
CTGACTGCAG ACCCAGACCA GATCACTAAC ATTGCTAAAA CCATAGACCC AGAGCTTTTA 
GGAAAGATGA ACTATCGGTT AATGATGTTA CAGTCCTGTT CTGGGCCAAC CTGTCGCACT 
CCAGGGGTTT TTGACCCCGG ATACAGGTTT GACCCCCGTC TCATGTTCAG CAATCGCGGC 
AGTGTCAGGA CTCGAAGATT TTCCAAACAT CTTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Disease correction by AAV-mediated gene therapy in a new mouse model of mucopolysaccharidosis type IIID.
Human molecular genetics26(8)1535-1551(2017 04)
Roca C,Motas S,Marc? S,Ribera A,S?nchez V,S?nchez X,Bertolin J,Le?n X,P?rez J,Garcia M,Villacampa P,Ruberte J,Pujol A,Haurigot V,Bosch F

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Mucopolysaccharidosis type IIID: 12 new patients and 15 novel mutations.
Human mutation31(5)E1348-60(2010 May)
Valstar MJ,Bertoli-Avella AM,Wessels MW,Ruijter GJ,de Graaf B,Olmer R,Elfferich P,Neijs S,Kariminejad R,Suheyl Ezg? F,Tokatli A,Czartoryska B,Bosschaart AN,van den Bos-Terpstra F,Puissant H,B?rger F,Omran H,Eckert D,Filocamo M,Simeonov E,Willems PJ,Wevers RA,Niermeijer MF,Halley DJ,Poorthuis BJ,van Diggelen OP

A novel loss-of-function mutation in the GNS gene causes Sanfilippo syndrome type D.
Genetic counseling (Geneva, Switzerland)20(2)133-9(2009)
El?ioglu NH,Pawlik B,Colak B,Beck M,Wollnik B

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GNS cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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