AMY2A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AMY2A
Entrez Gene ID	279
Full Name	amylase, alpha 2A (pancreatic)
Synonyms	AMY2,PA
General protein information	Preferred Names amylase, alpha 2A (pancreatic) Names pancreatic alpha-amylase 1,4-alpha-D-glucan glucanohydrolase glycogenase pancreatic amylase alpha 2A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p21.1
Summary	This gene encodes a member of the alpha-amylase family of proteins. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen. This gene and several family members are present in a gene cluster on chromosome 1. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jan 2015].
Disorder MIM:	104650

mRNA and Protein(s)

mRNA	Protein	Name
NM_000699.3	NP_000690.1	pancreatic alpha-amylase precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC607460	XP_005642408.1
Canis lupus familiaris (dog)	LOC480825	XP_537942.2
Bos taurus (cattle)	AMY2B	NP_001030188.1
Bos taurus (cattle)	AMY2A	XP_002686210.1
Gallus gallus (chicken)	AMY2A	NP_001001473.2
Danio rerio (zebrafish)	zgc:92137	NP_001003729.1
Pan troglodytes (chimpanzee)	AMY2A	NP_001103628.1
Rattus norvegicus (Norway rat)	Amy1a	NP_001010970.1
Xenopus tropicalis (tropical clawed frog)	amy2b	NP_001007948.1
Gallus gallus (chicken)	AMY1A	NP_001001528.1
Danio rerio (zebrafish)	zgc:66313	NP_956722.1
Homo sapiens (human)	AMY2A	NP_000690.1
	AMY2A	XP_001109826.1
Mus musculus (house mouse)	Amy1	NP_031472.2

Gene Ontology
Bibliography

Related articles in PubMed

Starch Digestion-Related Amylase Genetic Variant Affects 2-Year Changes in Adiposity in Response to Weight-Loss Diets: The POUNDS Lost Trial.
Heianza Y, Sun D, Wang T, Huang T, Bray GA, Sacks FM, Qi L
Diabetes66(9)2416-2423(2017 09)

Relationships Between Fasting Serum Amylase and Ghrelin or Peptide YY3-36 Levels in Healthy Men.
Tak YJ, Yi YH, Lee SY, Kim YJ, Lee JG, Cho YH
Pancreas45(3)376-80(2016 Mar)

Role of Amylase-?2A Autoantibodies in the Diagnosis of Autoimmune Pancreatitis.
S?nchez Casta??n M, Zuliani V, Amodio A, Campagnola P, Granato A, Gabbrielli A, Benini L, L?pez Hoyos M, Frulloni L
Pancreas44(7)1078-82(2015 Oct)

An association-adjusted consensus deleterious scheme to classify homozygous Mis-sense mutations for personal genome interpretation.
Preeprem T, Gibson G
BioData mining6(1)24(2013 Dec)

The Role of Copy Number Variation in African Americans with Type 2 Diabetes-Associated End Stage Renal Disease.
Bailey JN, Lu L, Chou JW, Xu J, McWilliams DR, Howard TD, Freedman BI, Bowden DW, Langefeld CD, Palmer ND
Journal of molecular and genetic medicine : an international journal of biomedical research761(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It was concluded that the genetic variant determining starch metabolism influences the response to weight-loss dietary intervention. Overweight and obese individuals carrying the AMY1-AMY2 rs11185098 genotype associated with higher amylase activity may have greater loss of adiposity during low-calorie diet interventions.
Title: Starch Digestion-Related Amylase Genetic Variant Affects 2-Year Changes in Adiposity in Response to Weight-Loss Diets: The POUNDS Lost Trial.

Data show that serum amylase was found to be correlated with waist circumference, ghrelin and PYY3-36.
Title: Relationships Between Fasting Serum Amylase and Ghrelin or Peptide YY3-36 Levels in Healthy Men.

Data show that amylase-alpha2A autoantibodies may help to diagnosis of autoimmune pancreatitis (AIP) and to differentiate AIP subtypes.
Title: Role of Amylase-?2A Autoantibodies in the Diagnosis of Autoimmune Pancreatitis.

Mammalian pancreatic alpha-amylases share a common carbohydrate binding activity and specifically bind to the intestinal brush border.
Title: Functional regulation of sugar assimilation by N-glycan-specific interaction of pancreatic ?-amylase with glycoproteins of duodenal brush border membrane.

Studies indicate that chloride activates alpha-amylases and ACE, and gaining insight into the potential mechanisms by which chloride functions in PSII.
Title: Chloride regulation of enzyme turnover: application to the role of chloride in photosystem II.

The following AMY2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AMY2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu02582	NM_000699.3 Latest version!	Homo sapiens amylase alpha 2A (AMY2A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AMY2A ( NM_000699.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000690.1 Homo sapiens amylase alpha 2A (AMY2A), mRNA.

Price & Availability↑

CloneID	OHu02582
Clone ID Related Accession (Same CDS sequence)	NM_000699.3
Accession Version	NM_000699.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	pancreatic alpha-amylase precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC146997.1, AK223463.1, M28443.1 and AW873834.1. On Jan 14, 2015 this sequence version replaced NM_000699.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007060.1, AK223463.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGTTCT TTCTGTTGCT TTTCACCATT GGGTTCTGCT GGGCTCAGTA TTCCCCAAAT 
ACACAACAAG GACGGACATC TATTGTTCAT CTGTTTGAAT GGCGATGGGT TGATATTGCT 
CTTGAATGTG AGCGATATTT AGCTCCGAAG GGATTTGGAG GGGTTCAGGT CTCTCCACCA 
AATGAAAATG TTGCAATTTA CAACCCTTTC AGACCTTGGT GGGAAAGATA CCAACCAGTT 
AGCTATAAAT TATGCACAAG ATCTGGAAAT GAAGATGAAT TTAGAAACAT GGTGACTAGA 
TGTAACAATG TTGGGGTTCG TATTTATGTG GATGCTGTAA TTAATCATAT GTGTGGTAAC 
GCTGTGAGTG CAGGAACAAG CAGTACCTGT GGAAGTTACT TCAACCCTGG AAGTAGGGAC 
TTTCCAGCAG TCCCATATTC TGGATGGGAT TTCAATGATG GTAAATGTAA AACTGGAAGT 
GGAGATATCG AGAACTACAA TGATGCTACT CAGGTCAGAG ATTGTCGTCT GACTGGTCTT 
CTTGATCTTG CACTGGAGAA GGATTACGTG CGTTCTAAGA TTGCCGAATA TATGAACCAT 
CTCATTGACA TTGGTGTTGC AGGGTTCAGA CTTGATGCTT CCAAGCACAT GTGGCCTGGA 
GACATAAAGG CAATTTTGGA CAAACTGCAT AATCTAAACA GTAACTGGTT CCCTGCAGGA 
AGTAAACCTT TCATTTACCA GGAGGTAATT GATCTGGGTG GTGAGCCAAT TAAAAGCAGT 
GACTACTTTG GTAATGGCCG GGTGACAGAA TTCAAGTATG GTGCAAAACT CGGCACAGTT 
ATTCGCAAGT GGAATGGAGA GAAGATGTCT TACTTAAAGA ACTGGGGAGA AGGTTGGGGT 
TTCGTACCTT CTGACAGAGC GCTTGTCTTT GTGGATAACC ATGACAATCA ACGAGGACAT 
GGGGCTGGAG GAGCCTCTAT TCTTACCTTC TGGGATGCTA GGCTGTACAA AATGGCAGTT 
GGATTTATGC TTGCTCATCC TTACGGATTT ACACGAGTAA TGTCAAGCTA CCGTTGGCCA 
AGACAGTTTC AAAATGGAAA CGATGTTAAT GATTGGGTTG GGCCACCAAA TAATAATGGA 
GTAATTAAAG AAGTTACTAT TAATCCAGAC ACTACTTGTG GCAATGACTG GGTCTGTGAA 
CATCGATGGC GCCAAATAAG GAACATGGTT ATTTTCCGCA ATGTAGTGGA TGGCCAGCCT 
TTTACAAATT GGTATGATAA TGGGAGCAAC CAAGTGGCTT TTGGGAGAGG AAACAGAGGA 
TTCATTGTTT TCAACAATGA TGACTGGTCA TTTTCTTTAA CTTTGCAAAC TGGTCTTCCT 
GCTGGCACAT ACTGTGATGT CATTTCTGGA GATAAAATTA ATGGCAATTG CACAGGCATT 
AAAATTTACG TTTCTGATGA TGGCAAAGCT CATTTTTCTA TTAGTAACTC TGCTGAAGAT 
CCATTTATTG CAATTCATGC TGAATCTAAA TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000690.1
CDS	110..1645
Translation	MKFFLLLFTIGFCWAQYSPNTQQGRTSIVHLFEWRWVDIALECERYLAPKGFGGVQVSPPNENVAIYNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCNNVGVRIYVDAVINHMCGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGSGDIENYNDATQVRDCRLTGLLDLALEKDYVRSKIAEYMNHLIDIGVAGFRLDASKHMWPGDIKAILDKLHNLNSNWFPAGSKPFIYQEVIDLGGEPIKSSDYFGNGRVTEFKYGAKLGTVIRKWNGEKMSYLKNWGEGWGFVPSDRALVFVDNHDNQRGHGAGGASILTFWDARLYKMAVGFMLAHPYGFTRVMSSYRWPRQFQNGNDVNDWVGPPNNNGVIKEVTINPDTTCGNDWVCEHRWRQIRNMVIFRNVVDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWSFSLTLQTGLPAGTYCDVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAESKL

Target ORF information:

RefSeq Version	NM_000699.3
Organism	Homo sapiens(human)
Definition	Homo sapiens amylase alpha 2A (AMY2A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000699.3

ORF Insert Sequence:

ATGAAGTTCT TTCTGTTGCT TTTCACCATT GGGTTCTGCT GGGCTCAGTA TTCCCCAAAT 
ACACAACAAG GACGGACATC TATTGTTCAT CTGTTTGAAT GGCGATGGGT TGATATTGCT 
CTTGAATGTG AGCGATATTT AGCTCCGAAG GGATTTGGAG GGGTTCAGGT CTCTCCACCA 
AATGAAAATG TTGCAATTTA CAACCCTTTC AGACCTTGGT GGGAAAGATA CCAACCAGTT 
AGCTATAAAT TATGCACAAG ATCTGGAAAT GAAGATGAAT TTAGAAACAT GGTGACTAGA 
TGTAACAATG TTGGGGTTCG TATTTATGTG GATGCTGTAA TTAATCATAT GTGTGGTAAC 
GCTGTGAGTG CAGGAACAAG CAGTACCTGT GGAAGTTACT TCAACCCTGG AAGTAGGGAC 
TTTCCAGCAG TCCCATATTC TGGATGGGAT TTCAATGATG GTAAATGTAA AACTGGAAGT 
GGAGATATCG AGAACTACAA TGATGCTACT CAGGTCAGAG ATTGTCGTCT GACTGGTCTT 
CTTGATCTTG CACTGGAGAA GGATTACGTG CGTTCTAAGA TTGCCGAATA TATGAACCAT 
CTCATTGACA TTGGTGTTGC AGGGTTCAGA CTTGATGCTT CCAAGCACAT GTGGCCTGGA 
GACATAAAGG CAATTTTGGA CAAACTGCAT AATCTAAACA GTAACTGGTT CCCTGCAGGA 
AGTAAACCTT TCATTTACCA GGAGGTAATT GATCTGGGTG GTGAGCCAAT TAAAAGCAGT 
GACTACTTTG GTAATGGCCG GGTGACAGAA TTCAAGTATG GTGCAAAACT CGGCACAGTT 
ATTCGCAAGT GGAATGGAGA GAAGATGTCT TACTTAAAGA ACTGGGGAGA AGGTTGGGGT 
TTCGTACCTT CTGACAGAGC GCTTGTCTTT GTGGATAACC ATGACAATCA ACGAGGACAT 
GGGGCTGGAG GAGCCTCTAT TCTTACCTTC TGGGATGCTA GGCTGTACAA AATGGCAGTT 
GGATTTATGC TTGCTCATCC TTACGGATTT ACACGAGTAA TGTCAAGCTA CCGTTGGCCA 
AGACAGTTTC AAAATGGAAA CGATGTTAAT GATTGGGTTG GGCCACCAAA TAATAATGGA 
GTAATTAAAG AAGTTACTAT TAATCCAGAC ACTACTTGTG GCAATGACTG GGTCTGTGAA 
CATCGATGGC GCCAAATAAG GAACATGGTT ATTTTCCGCA ATGTAGTGGA TGGCCAGCCT 
TTTACAAATT GGTATGATAA TGGGAGCAAC CAAGTGGCTT TTGGGAGAGG AAACAGAGGA 
TTCATTGTTT TCAACAATGA TGACTGGTCA TTTTCTTTAA CTTTGCAAAC TGGTCTTCCT 
GCTGGCACAT ACTGTGATGT CATTTCTGGA GATAAAATTA ATGGCAATTG CACAGGCATT 
AAAATTTACG TTTCTGATGA TGGCAAAGCT CATTTTTCTA TTAGTAACTC TGCTGAAGAT 
CCATTTATTG CAATTCATGC TGAATCTAAA TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Starch Digestion-Related Amylase Genetic Variant Affects 2-Year Changes in Adiposity in Response to Weight-Loss Diets: The POUNDS Lost Trial.
Diabetes66(9)2416-2423(2017 09)
Heianza Y,Sun D,Wang T,Huang T,Bray GA,Sacks FM,Qi L

Relationships Between Fasting Serum Amylase and Ghrelin or Peptide YY3-36 Levels in Healthy Men.
Pancreas45(3)376-80(2016 Mar)
Tak YJ,Yi YH,Lee SY,Kim YJ,Lee JG,Cho YH

Role of Amylase-?2A Autoantibodies in the Diagnosis of Autoimmune Pancreatitis.
Pancreas44(7)1078-82(2015 Oct)
S?nchez Casta??n M,Zuliani V,Amodio A,Campagnola P,Granato A,Gabbrielli A,Benini L,L?pez Hoyos M,Frulloni L

An association-adjusted consensus deleterious scheme to classify homozygous Mis-sense mutations for personal genome interpretation.
BioData mining6(1)24(2013 Dec)
Preeprem T,Gibson G

The Role of Copy Number Variation in African Americans with Type 2 Diabetes-Associated End Stage Renal Disease.
Journal of molecular and genetic medicine : an international journal of biomedical research761(2013 Jul)
Bailey JN,Lu L,Chou JW,Xu J,McWilliams DR,Howard TD,Freedman BI,Bowden DW,Langefeld CD,Palmer ND

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AMY2A cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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