GNAT2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GNAT2
Entrez Gene ID	2780
Full Name	G protein subunit alpha transducin 2
Synonyms	ACHM4,GNATC
General protein information	Preferred Names G protein subunit alpha transducin 2 Names guanine nucleotide-binding protein G(t) subunit alpha-2 cone-type transducin alpha subunit guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2 transducin alpha-2 chain transducin, cone-specific, alpha polypeptide
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p13.3
Summary	Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in cones. [provided by RefSeq, Jul 2008].
Disorder MIM:	139340
Disorder Html:	Achromatopsia 4, 613856 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_005272.3	NP_005263.1	guanine nucleotide-binding protein G(t) subunit alpha-2
NM_001377295.1	NP_001364224.1	guanine nucleotide-binding protein G(t) subunit alpha-2
NM_005272.5	NP_005263.1	guanine nucleotide-binding protein G(t) subunit alpha-2
NM_005272.4	NP_005263.1	guanine nucleotide-binding protein G(t) subunit alpha-2
XM_011541264.2	XP_011539566.1	guanine nucleotide-binding protein G(t) subunit alpha-2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GNAT2	XP_524787.2
Rattus norvegicus (Norway rat)	Gnat2	NP_001102420.2
Canis lupus familiaris (dog)	GNAT2	XP_547240.2
Mus musculus (house mouse)	Gnat2	NP_032167.1
Gallus gallus (chicken)	GNAT2	NP_990021.1
Xenopus tropicalis (tropical clawed frog)	LOC100216126	NP_001135577.1
Homo sapiens (human)	GNAT2	NP_005263.1
Bos taurus (cattle)	GNAT2	NP_776751.1
	GNAT2	XP_001091580.1
Danio rerio (zebrafish)	gnat2	NP_571944.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular genetics of achromatopsia in Newfoundland reveal genetic heterogeneity, founder effects and the first cases of Jalili syndrome in North America.
Doucette L, Green J, Black C, Schwartzentruber J, Johnson GJ, Galutira D, Young TL
Ophthalmic genetics34(3)119-29(2013 Sep)

Genome-wide meta-analysis identifies 11 new loci for anthropometric traits and provides insights into genetic architecture.
Berndt SI, Gustafsson S, M?gi R, Ganna A, Wheeler E, Feitosa MF, Justice AE, Monda KL, Croteau-Chonka DC, Day FR, Esko T, Fall T, Ferreira T, Gentilini D, Jackson AU, Luan J, Randall JC, Vedantam S, Willer CJ, Winkler TW, Wood AR, Workalemahu T, Hu YJ, Lee SH, Liang L, Lin DY, Min JL, Neale BM, Thorleifsson G, Yang J, Albrecht E, Amin N, Bragg-Gresham JL, Cadby G, den Heijer M, Eklund N, Fischer K, Goel A, Hottenga JJ, Huffman JE, Jarick I, Johansson ?, Johnson T, Kanoni S, Kleber ME, K?nig IR, Kristiansson K, Kutalik Z, Lamina C, Lecoeur C, Li G, Mangino M, McArdle WL, Medina-Gomez C, M?ller-Nurasyid M, Ngwa JS, Nolte IM, Paternoster L, Pechlivanis S, Perola M, Peters MJ, Preuss M, Rose LM, Shi J, Shungin D, Smith AV, Strawbridge RJ, Surakka I, Teumer A, Trip MD, Tyrer J, Van Vliet-Ostaptchouk JV, Vandenput L, Waite LL, Zhao JH, Absher D, Asselbergs FW, Atalay M, Attwood AP, Balmforth AJ, Basart H, Beilby J, Bonnycastle LL, Brambilla P, Bruinenberg M, Campbell H, Chasman DI, Chines PS, Collins FS, Connell JM, Cookson WO, de Faire U, de Vegt F, Dei M, Dimitriou M, Edkins S, Estrada K, Evans DM, Farrall M, Ferrario MM, Ferri?res J, Franke L, Frau F, Gejman PV, Grallert H, Gr?nberg H, Gudnason V, Hall AS, Hall P, Hartikainen AL, Hayward C, Heard-Costa NL, Heath AC, Hebebrand J, Homuth G, Hu FB, Hunt SE, Hypp?nen E, Iribarren C, Jacobs KB, Jansson JO, Jula A, K?h?nen M, Kathiresan S, Kee F, Khaw KT, Kivim?ki M, Koenig W, Kraja AT, Kumari M, Kuulasmaa K, Kuusisto J, Laitinen JH, Lakka TA, Langenberg C, Launer LJ, Lind L, Lindstr?m J, Liu J, Liuzzi A, Lokki ML, Lorentzon M, Madden PA, Magnusson PK, Manunta P, Marek D, M?rz W, Mateo Leach I, McKnight B, Medland SE, Mihailov E, Milani L, Montgomery GW, Mooser V, M?hleisen TW, Munroe PB, Musk AW, Narisu N, Navis G, Nicholson G, Nohr EA, Ong KK, Oostra BA, Palmer CN, Palotie A, Peden JF, Pedersen N, Peters A, Polasek O, Pouta A, Pramstaller PP, Prokopenko I, P?tter C, Radhakrishnan A, Raitakari O, Rendon A, Rivadeneira F, Rudan I, Saaristo TE, Sambrook JG, Sanders AR, Sanna S, Saramies J, Schipf S, Schreiber S, Schunkert H, Shin SY, Signorini S, Sinisalo J, Skrobek B, Soranzo N, Stan??kov? A, Stark K, Stephens JC, Stirrups K, Stolk RP, Stumvoll M, Swift AJ, Theodoraki EV, Thorand B, Tregouet DA, Tremoli E, Van der Klauw MM, van Meurs JB, Vermeulen SH, Viikari J, Virtamo J, Vitart V, Waeber G, Wang Z, Wid?n E, Wild SH, Willemsen G, Winkelmann BR, Witteman JC, Wolffenbuttel BH, Wong A, Wright AF, Zillikens MC, Amouyel P, Boehm BO, Boerwinkle E, Boomsma DI, Caulfield MJ, Chanock SJ, Cupples LA, Cusi D, Dedoussis GV, Erdmann J, Eriksson JG, Franks PW, Froguel P, Gieger C, Gyllensten U, Hamsten A, Harris TB, Hengstenberg C, Hicks AA, Hingorani A, Hinney A, Hofman A, Hovingh KG, Hveem K, Illig T, Jarvelin MR, J?ckel KH, Keinanen-Kiukaanniemi SM, Kiemeney LA, Kuh D, Laakso M, Lehtim?ki T, Levinson DF, Martin NG, Metspalu A, Morris AD, Nieminen MS, Nj?lstad I, Ohlsson C, Oldehinkel AJ, Ouwehand WH, Palmer LJ, Penninx B, Power C, Province MA, Psaty BM, Qi L, Rauramaa R, Ridker PM, Ripatti S, Salomaa V, Samani NJ, Snieder H, S?rensen TI, Spector TD, Stefansson K, T?njes A, Tuomilehto J, Uitterlinden AG, Uusitupa M, van der Harst P, Vollenweider P, Wallaschofski H, Wareham NJ, Watkins H, Wichmann HE, Wilson JF, Abecasis GR, Assimes TL, Barroso I, Boehnke M, Borecki IB, Deloukas P, Fox CS, Frayling T, Groop LC, Haritunian T, Heid IM, Hunter D, Kaplan RC, Karpe F, Moffatt MF, Mohlke KL, O'Connell JR, Pawitan Y, Schadt EE, Schlessinger D, Steinthorsdottir V, Strachan DP, Thorsteinsdottir U, van Duijn CM, Visscher PM, Di Blasio AM, Hirschhorn JN, Lindgren CM, Morris AP, Meyre D, Scherag A, McCarthy MI, Speliotes EK, North KE, Loos RJ, Ingelsson E
Nature genetics45(5)501-12(2013 May)

Clinical utility gene card for: achromatopsia.
Kohl S, Hamel CP
European journal of human genetics : EJHG19(6)(2011 Jun)

Clinical and genetic investigation of a large Tunisian family with complete achromatopsia: identification of a new nonsense mutation in GNAT2 gene.
Ouechtati F, Merdassi A, Bouyacoub Y, Largueche L, Derouiche K, Ouragini H, Nouira S, Tiab L, Baklouti K, Rebai A, Schorderet DF, Munier FL, Zografos L, Abdelhak S, El Matri L
Journal of human genetics56(1)22-8(2011 Jan)

Replacing the rod with the cone transducin subunit decreases sensitivity and accelerates response decay.
Chen CK, Woodruff ML, Chen FS, Shim H, Cilluffo MC, Fain GL
The Journal of physiology588(Pt 17)3231-41(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The majority (n = 12) of patients were either homozygotes or compound heterozygotes for known achromatopsia alleles, two in CNGB3 (p.T383fsX and p.T296YfsX9) and three in CNGA3 (p.R283Q, p.R427C and p.L527R).
Title: Molecular genetics of achromatopsia in Newfoundland reveal genetic heterogeneity, founder effects and the first cases of Jalili syndrome in North America.

Single nucleotide polymorphisms in GNAT2 gene is associated with obesity.
Title: Genome-wide meta-analysis identifies 11 new loci for anthropometric traits and provides insights into genetic architecture.

Missense mutations, nonsense mutations, splice mutations, and small deletions and insertions in the affected genes cause achromatopsia.
Title: Clinical utility gene card for: achromatopsia.

Clinical and genetic investigation of a large Tunisian family with complete achromatopsia: identification of a new nonsense mutation in GNAT2 gene.
Title: Clinical and genetic investigation of a large Tunisian family with complete achromatopsia: identification of a new nonsense mutation in GNAT2 gene.

Expression of GNAT2 transgene, when found in rod photoreceptor cells rather than in cones, demonstrates different mechanisms of amplification in the body's G-protein alpha cascades and the activation of phosphodiesterase 6.
Title: Replacing the rod with the cone transducin subunit decreases sensitivity and accelerates response decay.

The following GNAT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNAT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19578	XM_011541264.2 Latest version!	Homo sapiens G protein subunit alpha transducin 2 (GNAT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu19578	NM_005272.5 Latest version!	Homo sapiens G protein subunit alpha transducin 2 (GNAT2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu19578	NM_001377295.1 Latest version!	Homo sapiens G protein subunit alpha transducin 2 (GNAT2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu19578	NM_005272.3	Homo sapiens G protein subunit alpha transducin 2 (GNAT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu19578	NM_005272.4	Homo sapiens G protein subunit alpha transducin 2 (GNAT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNAT2 ( NM_005272.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005263.1 Homo sapiens G protein subunit alpha transducin 2 (GNAT2), mRNA.

Price & Availability↑

CloneID	OHu19578
Clone ID Related Accession (Same CDS sequence)	NM_005272.3 , NM_001377295.1 , NM_005272.5 , NM_005272.4 , XM_011541264.2
Accession Version	NM_005272.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(t) subunit alpha-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355310.20, AF493909.1 and BC000233.1. This sequence is a reference standard in the RefSeqGene project. On Jun 15, 2006 this sequence version replaced NM_005272.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000233.1, AF493909.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAAGTG GAGCCAGTGC TGAGGACAAA GAACTGGCCA AGAGGTCCAA GGAGCTAGAA 
AAGAAGCTGC AGGAGGATGC TGATAAGGAA GCCAAGACTG TCAAGCTGCT ACTGCTGGGT 
GCTGGGGAGT CAGGAAAGAG CACCATCGTC AAACAGATGA AGATCATTCA CCAGGATGGC 
TATTCACCAG AAGAATGCCT GGAGTTCAAG GCTATCATCT ATGGAAATGT GCTGCAGTCC 
ATCCTGGCTA TCATCCGGGC CATGACCACA CTGGGCATCG ATTATGCTGA ACCAAGCTGT 
GCGGATGACG GGCGACAGCT CAACAACCTG GCTGACTCCA TTGAGGAGGG AACCATGCCT 
CCTGAGCTCG TGGAGGTCAT TAGGAGGTTG TGGAAGGATG GTGGGGTGCA AGCCTGCTTC 
GAGAGAGCTG CAGAATACCA GCTTAATGAC TCCGCATCTT ACTACCTGAA CCAATTAGAA 
CGAATTACAG ACCCTGAGTA CCTCCCTAGT GAGCAAGATG TGCTCCGATC CAGAGTCAAA 
ACCACGGGCA TCATTGAAAC CAAGTTTTCC GTCAAAGACT TGAATTTCAG GATGTTTGAT 
GTGGGAGGGC AGAGATCCGA GAGAAAGAAG TGGATCCACT GCTTCGAGGG AGTCACCTGC 
ATCATTTTCT GTGCAGCCCT CAGTGCCTAT GATATGGTGC TGGTGGAAGA TGACGAAGTG 
AATCGTATGC ATGAGTCTTT GCATCTGTTC AACAGCATAT GTAACCACAA ATTCTTTGCG 
GCTACTTCCA TTGTCCTCTT TCTCAACAAG AAGGACCTCT TTGAGGAAAA AATCAAGAAA 
GTCCATCTCA GCATTTGTTT TCCAGAGTAT GATGGTAACA ACTCCTATGA TGATGCGGGG 
AATTACATAA AGAGCCAGTT CCTTGACCTC AATATGCGAA AAGATGTCAA AGAAATCTAC 
AGTCACATGA CCTGTGCTAC AGATACACAG AATGTCAAAT TTGTGTTTGA TGCAGTTACA 
GATATTATCA TCAAAGAAAA CCTCAAGGAC TGCGGCCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005263.1
CDS	214..1278
Translation	MGSGASAEDKELAKRSKELEKKLQEDADKEAKTVKLLLLGAGESGKSTIVKQMKIIHQDGYSPEECLEFKAIIYGNVLQSILAIIRAMTTLGIDYAEPSCADDGRQLNNLADSIEEGTMPPELVEVIRRLWKDGGVQACFERAAEYQLNDSASYYLNQLERITDPEYLPSEQDVLRSRVKTTGIIETKFSVKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFCAALSAYDMVLVEDDEVNRMHESLHLFNSICNHKFFAATSIVLFLNKKDLFEEKIKKVHLSICFPEYDGNNSYDDAGNYIKSQFLDLNMRKDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	NM_005272.3
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit alpha transducin 2 (GNAT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005272.3

ORF Insert Sequence:

ATGGGAAGTG GAGCCAGTGC TGAGGACAAA GAACTGGCCA AGAGGTCCAA GGAGCTAGAA 
AAGAAGCTGC AGGAGGATGC TGATAAGGAA GCCAAGACTG TCAAGCTGCT ACTGCTGGGT 
GCTGGGGAGT CAGGAAAGAG CACCATCGTC AAACAGATGA AGATCATTCA CCAGGATGGC 
TATTCACCAG AAGAATGCCT GGAGTTCAAG GCTATCATCT ATGGAAATGT GCTGCAGTCC 
ATCCTGGCTA TCATCCGGGC CATGACCACA CTGGGCATCG ATTATGCTGA ACCAAGCTGT 
GCGGATGACG GGCGACAGCT CAACAACCTG GCTGACTCCA TTGAGGAGGG AACCATGCCT 
CCTGAGCTCG TGGAGGTCAT TAGGAGGTTG TGGAAGGATG GTGGGGTGCA AGCCTGCTTC 
GAGAGAGCTG CAGAATACCA GCTTAATGAC TCCGCATCTT ACTACCTGAA CCAATTAGAA 
CGAATTACAG ACCCTGAGTA CCTCCCTAGT GAGCAAGATG TGCTCCGATC CAGAGTCAAA 
ACCACGGGCA TCATTGAAAC CAAGTTTTCC GTCAAAGACT TGAATTTCAG GATGTTTGAT 
GTGGGAGGGC AGAGATCCGA GAGAAAGAAG TGGATCCACT GCTTCGAGGG AGTCACCTGC 
ATCATTTTCT GTGCAGCCCT CAGTGCCTAT GATATGGTGC TGGTGGAAGA TGACGAAGTG 
AATCGTATGC ATGAGTCTTT GCATCTGTTC AACAGCATAT GTAACCACAA ATTCTTTGCG 
GCTACTTCCA TTGTCCTCTT TCTCAACAAG AAGGACCTCT TTGAGGAAAA AATCAAGAAA 
GTCCATCTCA GCATTTGTTT TCCAGAGTAT GATGGTAACA ACTCCTATGA TGATGCGGGG 
AATTACATAA AGAGCCAGTT CCTTGACCTC AATATGCGAA AAGATGTCAA AGAAATCTAC 
AGTCACATGA CCTGTGCTAC AGATACACAG AATGTCAAAT TTGTGTTTGA TGCAGTTACA 
GATATTATCA TCAAAGAAAA CCTCAAGGAC TGCGGCCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAT2 ( NM_005272.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005263.1 Homo sapiens G protein subunit alpha transducin 2 (GNAT2), mRNA.

Price & Availability↑

CloneID	OHu19578
Clone ID Related Accession (Same CDS sequence)	NM_005272.3 , NM_001377295.1 , NM_005272.5 , NM_005272.4 , XM_011541264.2
Accession Version	NM_005272.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(t) subunit alpha-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355310.20, AF493909.1 and BC000233.1. On May 17, 2019 this sequence version replaced NM_005272.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000233.1, AF493909.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGAAGTG GAGCCAGTGC TGAGGACAAA GAACTGGCCA AGAGGTCCAA GGAGCTAGAA 
AAGAAGCTGC AGGAGGATGC TGATAAGGAA GCCAAGACTG TCAAGCTGCT ACTGCTGGGT 
GCTGGGGAGT CAGGAAAGAG CACCATCGTC AAACAGATGA AGATCATTCA CCAGGATGGC 
TATTCACCAG AAGAATGCCT GGAGTTCAAG GCTATCATCT ATGGAAATGT GCTGCAGTCC 
ATCCTGGCTA TCATCCGGGC CATGACCACA CTGGGCATCG ATTATGCTGA ACCAAGCTGT 
GCGGATGACG GGCGACAGCT CAACAACCTG GCTGACTCCA TTGAGGAGGG AACCATGCCT 
CCTGAGCTCG TGGAGGTCAT TAGGAGGTTG TGGAAGGATG GTGGGGTGCA AGCCTGCTTC 
GAGAGAGCTG CAGAATACCA GCTTAATGAC TCCGCATCTT ACTACCTGAA CCAATTAGAA 
CGAATTACAG ACCCTGAGTA CCTCCCTAGT GAGCAAGATG TGCTCCGATC CAGAGTCAAA 
ACCACGGGCA TCATTGAAAC CAAGTTTTCC GTCAAAGACT TGAATTTCAG GATGTTTGAT 
GTGGGAGGGC AGAGATCCGA GAGAAAGAAG TGGATCCACT GCTTCGAGGG AGTCACCTGC 
ATCATTTTCT GTGCAGCCCT CAGTGCCTAT GATATGGTGC TGGTGGAAGA TGACGAAGTG 
AATCGTATGC ATGAGTCTTT GCATCTGTTC AACAGCATAT GTAACCACAA ATTCTTTGCG 
GCTACTTCCA TTGTCCTCTT TCTCAACAAG AAGGACCTCT TTGAGGAAAA AATCAAGAAA 
GTCCATCTCA GCATTTGTTT TCCAGAGTAT GATGGTAACA ACTCCTATGA TGATGCGGGG 
AATTACATAA AGAGCCAGTT CCTTGACCTC AATATGCGAA AAGATGTCAA AGAAATCTAC 
AGTCACATGA CCTGTGCTAC AGATACACAG AATGTCAAAT TTGTGTTTGA TGCAGTTACA 
GATATTATCA TCAAAGAAAA CCTCAAGGAC TGCGGCCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005263.1
CDS	215..1279
Translation	MGSGASAEDKELAKRSKELEKKLQEDADKEAKTVKLLLLGAGESGKSTIVKQMKIIHQDGYSPEECLEFKAIIYGNVLQSILAIIRAMTTLGIDYAEPSCADDGRQLNNLADSIEEGTMPPELVEVIRRLWKDGGVQACFERAAEYQLNDSASYYLNQLERITDPEYLPSEQDVLRSRVKTTGIIETKFSVKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFCAALSAYDMVLVEDDEVNRMHESLHLFNSICNHKFFAATSIVLFLNKKDLFEEKIKKVHLSICFPEYDGNNSYDDAGNYIKSQFLDLNMRKDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	NM_005272.4
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit alpha transducin 2 (GNAT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005272.4

ORF Insert Sequence:

ATGGGAAGTG GAGCCAGTGC TGAGGACAAA GAACTGGCCA AGAGGTCCAA GGAGCTAGAA 
AAGAAGCTGC AGGAGGATGC TGATAAGGAA GCCAAGACTG TCAAGCTGCT ACTGCTGGGT 
GCTGGGGAGT CAGGAAAGAG CACCATCGTC AAACAGATGA AGATCATTCA CCAGGATGGC 
TATTCACCAG AAGAATGCCT GGAGTTCAAG GCTATCATCT ATGGAAATGT GCTGCAGTCC 
ATCCTGGCTA TCATCCGGGC CATGACCACA CTGGGCATCG ATTATGCTGA ACCAAGCTGT 
GCGGATGACG GGCGACAGCT CAACAACCTG GCTGACTCCA TTGAGGAGGG AACCATGCCT 
CCTGAGCTCG TGGAGGTCAT TAGGAGGTTG TGGAAGGATG GTGGGGTGCA AGCCTGCTTC 
GAGAGAGCTG CAGAATACCA GCTTAATGAC TCCGCATCTT ACTACCTGAA CCAATTAGAA 
CGAATTACAG ACCCTGAGTA CCTCCCTAGT GAGCAAGATG TGCTCCGATC CAGAGTCAAA 
ACCACGGGCA TCATTGAAAC CAAGTTTTCC GTCAAAGACT TGAATTTCAG GATGTTTGAT 
GTGGGAGGGC AGAGATCCGA GAGAAAGAAG TGGATCCACT GCTTCGAGGG AGTCACCTGC 
ATCATTTTCT GTGCAGCCCT CAGTGCCTAT GATATGGTGC TGGTGGAAGA TGACGAAGTG 
AATCGTATGC ATGAGTCTTT GCATCTGTTC AACAGCATAT GTAACCACAA ATTCTTTGCG 
GCTACTTCCA TTGTCCTCTT TCTCAACAAG AAGGACCTCT TTGAGGAAAA AATCAAGAAA 
GTCCATCTCA GCATTTGTTT TCCAGAGTAT GATGGTAACA ACTCCTATGA TGATGCGGGG 
AATTACATAA AGAGCCAGTT CCTTGACCTC AATATGCGAA AAGATGTCAA AGAAATCTAC 
AGTCACATGA CCTGTGCTAC AGATACACAG AATGTCAAAT TTGTGTTTGA TGCAGTTACA 
GATATTATCA TCAAAGAAAA CCTCAAGGAC TGCGGCCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAT2 ( XM_011541264.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539566.1 Homo sapiens G protein subunit alpha transducin 2 (GNAT2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu19578
Clone ID Related Accession (Same CDS sequence)	NM_005272.3 , NM_001377295.1 , NM_005272.5 , NM_005272.4 , XM_011541264.2
Accession Version	XM_011541264.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(t) subunit alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541264.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGGAAGTG GAGCCAGTGC TGAGGACAAA GAACTGGCCA AGAGGTCCAA GGAGCTAGAA 
AAGAAGCTGC AGGAGGATGC TGATAAGGAA GCCAAGACTG TCAAGCTGCT ACTGCTGGGT 
GCTGGGGAGT CAGGAAAGAG CACCATCGTC AAACAGATGA AGATCATTCA CCAGGATGGC 
TATTCACCAG AAGAATGCCT GGAGTTCAAG GCTATCATCT ATGGAAATGT GCTGCAGTCC 
ATCCTGGCTA TCATCCGGGC CATGACCACA CTGGGCATCG ATTATGCTGA ACCAAGCTGT 
GCGGATGACG GGCGACAGCT CAACAACCTG GCTGACTCCA TTGAGGAGGG AACCATGCCT 
CCTGAGCTCG TGGAGGTCAT TAGGAGGTTG TGGAAGGATG GTGGGGTGCA AGCCTGCTTC 
GAGAGAGCTG CAGAATACCA GCTTAATGAC TCCGCATCTT ACTACCTGAA CCAATTAGAA 
CGAATTACAG ACCCTGAGTA CCTCCCTAGT GAGCAAGATG TGCTCCGATC CAGAGTCAAA 
ACCACGGGCA TCATTGAAAC CAAGTTTTCC GTCAAAGACT TGAATTTCAG GATGTTTGAT 
GTGGGAGGGC AGAGATCCGA GAGAAAGAAG TGGATCCACT GCTTCGAGGG AGTCACCTGC 
ATCATTTTCT GTGCAGCCCT CAGTGCCTAT GATATGGTGC TGGTGGAAGA TGACGAAGTG 
AATCGTATGC ATGAGTCTTT GCATCTGTTC AACAGCATAT GTAACCACAA ATTCTTTGCG 
GCTACTTCCA TTGTCCTCTT TCTCAACAAG AAGGACCTCT TTGAGGAAAA AATCAAGAAA 
GTCCATCTCA GCATTTGTTT TCCAGAGTAT GATGGTAACA ACTCCTATGA TGATGCGGGG 
AATTACATAA AGAGCCAGTT CCTTGACCTC AATATGCGAA AAGATGTCAA AGAAATCTAC 
AGTCACATGA CCTGTGCTAC AGATACACAG AATGTCAAAT TTGTGTTTGA TGCAGTTACA 
GATATTATCA TCAAAGAAAA CCTCAAGGAC TGCGGCCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539566.1
CDS	305..1369
Translation	MGSGASAEDKELAKRSKELEKKLQEDADKEAKTVKLLLLGAGESGKSTIVKQMKIIHQDGYSPEECLEFKAIIYGNVLQSILAIIRAMTTLGIDYAEPSCADDGRQLNNLADSIEEGTMPPELVEVIRRLWKDGGVQACFERAAEYQLNDSASYYLNQLERITDPEYLPSEQDVLRSRVKTTGIIETKFSVKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFCAALSAYDMVLVEDDEVNRMHESLHLFNSICNHKFFAATSIVLFLNKKDLFEEKIKKVHLSICFPEYDGNNSYDDAGNYIKSQFLDLNMRKDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	XM_011541264.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit alpha transducin 2 (GNAT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541264.2

ORF Insert Sequence:

ATGGGAAGTG GAGCCAGTGC TGAGGACAAA GAACTGGCCA AGAGGTCCAA GGAGCTAGAA 
AAGAAGCTGC AGGAGGATGC TGATAAGGAA GCCAAGACTG TCAAGCTGCT ACTGCTGGGT 
GCTGGGGAGT CAGGAAAGAG CACCATCGTC AAACAGATGA AGATCATTCA CCAGGATGGC 
TATTCACCAG AAGAATGCCT GGAGTTCAAG GCTATCATCT ATGGAAATGT GCTGCAGTCC 
ATCCTGGCTA TCATCCGGGC CATGACCACA CTGGGCATCG ATTATGCTGA ACCAAGCTGT 
GCGGATGACG GGCGACAGCT CAACAACCTG GCTGACTCCA TTGAGGAGGG AACCATGCCT 
CCTGAGCTCG TGGAGGTCAT TAGGAGGTTG TGGAAGGATG GTGGGGTGCA AGCCTGCTTC 
GAGAGAGCTG CAGAATACCA GCTTAATGAC TCCGCATCTT ACTACCTGAA CCAATTAGAA 
CGAATTACAG ACCCTGAGTA CCTCCCTAGT GAGCAAGATG TGCTCCGATC CAGAGTCAAA 
ACCACGGGCA TCATTGAAAC CAAGTTTTCC GTCAAAGACT TGAATTTCAG GATGTTTGAT 
GTGGGAGGGC AGAGATCCGA GAGAAAGAAG TGGATCCACT GCTTCGAGGG AGTCACCTGC 
ATCATTTTCT GTGCAGCCCT CAGTGCCTAT GATATGGTGC TGGTGGAAGA TGACGAAGTG 
AATCGTATGC ATGAGTCTTT GCATCTGTTC AACAGCATAT GTAACCACAA ATTCTTTGCG 
GCTACTTCCA TTGTCCTCTT TCTCAACAAG AAGGACCTCT TTGAGGAAAA AATCAAGAAA 
GTCCATCTCA GCATTTGTTT TCCAGAGTAT GATGGTAACA ACTCCTATGA TGATGCGGGG 
AATTACATAA AGAGCCAGTT CCTTGACCTC AATATGCGAA AAGATGTCAA AGAAATCTAC 
AGTCACATGA CCTGTGCTAC AGATACACAG AATGTCAAAT TTGTGTTTGA TGCAGTTACA 
GATATTATCA TCAAAGAAAA CCTCAAGGAC TGCGGCCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAT2 ( NM_001377295.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001364224.1 Homo sapiens G protein subunit alpha transducin 2 (GNAT2), transcript variant 2, mRNA.

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CloneID	OHu19578
Clone ID Related Accession (Same CDS sequence)	NM_005272.3 , NM_001377295.1 , NM_005272.5 , NM_005272.4 , XM_011541264.2
Accession Version	NM_001377295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-05
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(t) subunit alpha-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355310.20. On Jan 1, 2020 this sequence version replaced XM_011541264.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.130247.1, SRR1163655.684157.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGAAGTG GAGCCAGTGC TGAGGACAAA GAACTGGCCA AGAGGTCCAA GGAGCTAGAA 
AAGAAGCTGC AGGAGGATGC TGATAAGGAA GCCAAGACTG TCAAGCTGCT ACTGCTGGGT 
GCTGGGGAGT CAGGAAAGAG CACCATCGTC AAACAGATGA AGATCATTCA CCAGGATGGC 
TATTCACCAG AAGAATGCCT GGAGTTCAAG GCTATCATCT ATGGAAATGT GCTGCAGTCC 
ATCCTGGCTA TCATCCGGGC CATGACCACA CTGGGCATCG ATTATGCTGA ACCAAGCTGT 
GCGGATGACG GGCGACAGCT CAACAACCTG GCTGACTCCA TTGAGGAGGG AACCATGCCT 
CCTGAGCTCG TGGAGGTCAT TAGGAGGTTG TGGAAGGATG GTGGGGTGCA AGCCTGCTTC 
GAGAGAGCTG CAGAATACCA GCTTAATGAC TCCGCATCTT ACTACCTGAA CCAATTAGAA 
CGAATTACAG ACCCTGAGTA CCTCCCTAGT GAGCAAGATG TGCTCCGATC CAGAGTCAAA 
ACCACGGGCA TCATTGAAAC CAAGTTTTCC GTCAAAGACT TGAATTTCAG GATGTTTGAT 
GTGGGAGGGC AGAGATCCGA GAGAAAGAAG TGGATCCACT GCTTCGAGGG AGTCACCTGC 
ATCATTTTCT GTGCAGCCCT CAGTGCCTAT GATATGGTGC TGGTGGAAGA TGACGAAGTG 
AATCGTATGC ATGAGTCTTT GCATCTGTTC AACAGCATAT GTAACCACAA ATTCTTTGCG 
GCTACTTCCA TTGTCCTCTT TCTCAACAAG AAGGACCTCT TTGAGGAAAA AATCAAGAAA 
GTCCATCTCA GCATTTGTTT TCCAGAGTAT GATGGTAACA ACTCCTATGA TGATGCGGGG 
AATTACATAA AGAGCCAGTT CCTTGACCTC AATATGCGAA AAGATGTCAA AGAAATCTAC 
AGTCACATGA CCTGTGCTAC AGATACACAG AATGTCAAAT TTGTGTTTGA TGCAGTTACA 
GATATTATCA TCAAAGAAAA CCTCAAGGAC TGCGGCCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001364224.1
CDS	110..1174
Translation	MGSGASAEDKELAKRSKELEKKLQEDADKEAKTVKLLLLGAGESGKSTIVKQMKIIHQDGYSPEECLEFKAIIYGNVLQSILAIIRAMTTLGIDYAEPSCADDGRQLNNLADSIEEGTMPPELVEVIRRLWKDGGVQACFERAAEYQLNDSASYYLNQLERITDPEYLPSEQDVLRSRVKTTGIIETKFSVKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFCAALSAYDMVLVEDDEVNRMHESLHLFNSICNHKFFAATSIVLFLNKKDLFEEKIKKVHLSICFPEYDGNNSYDDAGNYIKSQFLDLNMRKDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	NM_001377295.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit alpha transducin 2 (GNAT2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377295.1

ORF Insert Sequence:

ATGGGAAGTG GAGCCAGTGC TGAGGACAAA GAACTGGCCA AGAGGTCCAA GGAGCTAGAA 
AAGAAGCTGC AGGAGGATGC TGATAAGGAA GCCAAGACTG TCAAGCTGCT ACTGCTGGGT 
GCTGGGGAGT CAGGAAAGAG CACCATCGTC AAACAGATGA AGATCATTCA CCAGGATGGC 
TATTCACCAG AAGAATGCCT GGAGTTCAAG GCTATCATCT ATGGAAATGT GCTGCAGTCC 
ATCCTGGCTA TCATCCGGGC CATGACCACA CTGGGCATCG ATTATGCTGA ACCAAGCTGT 
GCGGATGACG GGCGACAGCT CAACAACCTG GCTGACTCCA TTGAGGAGGG AACCATGCCT 
CCTGAGCTCG TGGAGGTCAT TAGGAGGTTG TGGAAGGATG GTGGGGTGCA AGCCTGCTTC 
GAGAGAGCTG CAGAATACCA GCTTAATGAC TCCGCATCTT ACTACCTGAA CCAATTAGAA 
CGAATTACAG ACCCTGAGTA CCTCCCTAGT GAGCAAGATG TGCTCCGATC CAGAGTCAAA 
ACCACGGGCA TCATTGAAAC CAAGTTTTCC GTCAAAGACT TGAATTTCAG GATGTTTGAT 
GTGGGAGGGC AGAGATCCGA GAGAAAGAAG TGGATCCACT GCTTCGAGGG AGTCACCTGC 
ATCATTTTCT GTGCAGCCCT CAGTGCCTAT GATATGGTGC TGGTGGAAGA TGACGAAGTG 
AATCGTATGC ATGAGTCTTT GCATCTGTTC AACAGCATAT GTAACCACAA ATTCTTTGCG 
GCTACTTCCA TTGTCCTCTT TCTCAACAAG AAGGACCTCT TTGAGGAAAA AATCAAGAAA 
GTCCATCTCA GCATTTGTTT TCCAGAGTAT GATGGTAACA ACTCCTATGA TGATGCGGGG 
AATTACATAA AGAGCCAGTT CCTTGACCTC AATATGCGAA AAGATGTCAA AGAAATCTAC 
AGTCACATGA CCTGTGCTAC AGATACACAG AATGTCAAAT TTGTGTTTGA TGCAGTTACA 
GATATTATCA TCAAAGAAAA CCTCAAGGAC TGCGGCCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAT2 ( NM_005272.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005263.1 Homo sapiens G protein subunit alpha transducin 2 (GNAT2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19578
Clone ID Related Accession (Same CDS sequence)	NM_005272.3 , NM_001377295.1 , NM_005272.5 , NM_005272.4 , XM_011541264.2
Accession Version	NM_005272.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-05
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(t) subunit alpha-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355310.20. On Jan 1, 2020 this sequence version replaced NM_005272.4. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000233.1, AF493909.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGAAGTG GAGCCAGTGC TGAGGACAAA GAACTGGCCA AGAGGTCCAA GGAGCTAGAA 
AAGAAGCTGC AGGAGGATGC TGATAAGGAA GCCAAGACTG TCAAGCTGCT ACTGCTGGGT 
GCTGGGGAGT CAGGAAAGAG CACCATCGTC AAACAGATGA AGATCATTCA CCAGGATGGC 
TATTCACCAG AAGAATGCCT GGAGTTCAAG GCTATCATCT ATGGAAATGT GCTGCAGTCC 
ATCCTGGCTA TCATCCGGGC CATGACCACA CTGGGCATCG ATTATGCTGA ACCAAGCTGT 
GCGGATGACG GGCGACAGCT CAACAACCTG GCTGACTCCA TTGAGGAGGG AACCATGCCT 
CCTGAGCTCG TGGAGGTCAT TAGGAGGTTG TGGAAGGATG GTGGGGTGCA AGCCTGCTTC 
GAGAGAGCTG CAGAATACCA GCTTAATGAC TCCGCATCTT ACTACCTGAA CCAATTAGAA 
CGAATTACAG ACCCTGAGTA CCTCCCTAGT GAGCAAGATG TGCTCCGATC CAGAGTCAAA 
ACCACGGGCA TCATTGAAAC CAAGTTTTCC GTCAAAGACT TGAATTTCAG GATGTTTGAT 
GTGGGAGGGC AGAGATCCGA GAGAAAGAAG TGGATCCACT GCTTCGAGGG AGTCACCTGC 
ATCATTTTCT GTGCAGCCCT CAGTGCCTAT GATATGGTGC TGGTGGAAGA TGACGAAGTG 
AATCGTATGC ATGAGTCTTT GCATCTGTTC AACAGCATAT GTAACCACAA ATTCTTTGCG 
GCTACTTCCA TTGTCCTCTT TCTCAACAAG AAGGACCTCT TTGAGGAAAA AATCAAGAAA 
GTCCATCTCA GCATTTGTTT TCCAGAGTAT GATGGTAACA ACTCCTATGA TGATGCGGGG 
AATTACATAA AGAGCCAGTT CCTTGACCTC AATATGCGAA AAGATGTCAA AGAAATCTAC 
AGTCACATGA CCTGTGCTAC AGATACACAG AATGTCAAAT TTGTGTTTGA TGCAGTTACA 
GATATTATCA TCAAAGAAAA CCTCAAGGAC TGCGGCCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005263.1
CDS	215..1279
Translation	MGSGASAEDKELAKRSKELEKKLQEDADKEAKTVKLLLLGAGESGKSTIVKQMKIIHQDGYSPEECLEFKAIIYGNVLQSILAIIRAMTTLGIDYAEPSCADDGRQLNNLADSIEEGTMPPELVEVIRRLWKDGGVQACFERAAEYQLNDSASYYLNQLERITDPEYLPSEQDVLRSRVKTTGIIETKFSVKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFCAALSAYDMVLVEDDEVNRMHESLHLFNSICNHKFFAATSIVLFLNKKDLFEEKIKKVHLSICFPEYDGNNSYDDAGNYIKSQFLDLNMRKDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	NM_005272.5
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit alpha transducin 2 (GNAT2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005272.5

ORF Insert Sequence:

ATGGGAAGTG GAGCCAGTGC TGAGGACAAA GAACTGGCCA AGAGGTCCAA GGAGCTAGAA 
AAGAAGCTGC AGGAGGATGC TGATAAGGAA GCCAAGACTG TCAAGCTGCT ACTGCTGGGT 
GCTGGGGAGT CAGGAAAGAG CACCATCGTC AAACAGATGA AGATCATTCA CCAGGATGGC 
TATTCACCAG AAGAATGCCT GGAGTTCAAG GCTATCATCT ATGGAAATGT GCTGCAGTCC 
ATCCTGGCTA TCATCCGGGC CATGACCACA CTGGGCATCG ATTATGCTGA ACCAAGCTGT 
GCGGATGACG GGCGACAGCT CAACAACCTG GCTGACTCCA TTGAGGAGGG AACCATGCCT 
CCTGAGCTCG TGGAGGTCAT TAGGAGGTTG TGGAAGGATG GTGGGGTGCA AGCCTGCTTC 
GAGAGAGCTG CAGAATACCA GCTTAATGAC TCCGCATCTT ACTACCTGAA CCAATTAGAA 
CGAATTACAG ACCCTGAGTA CCTCCCTAGT GAGCAAGATG TGCTCCGATC CAGAGTCAAA 
ACCACGGGCA TCATTGAAAC CAAGTTTTCC GTCAAAGACT TGAATTTCAG GATGTTTGAT 
GTGGGAGGGC AGAGATCCGA GAGAAAGAAG TGGATCCACT GCTTCGAGGG AGTCACCTGC 
ATCATTTTCT GTGCAGCCCT CAGTGCCTAT GATATGGTGC TGGTGGAAGA TGACGAAGTG 
AATCGTATGC ATGAGTCTTT GCATCTGTTC AACAGCATAT GTAACCACAA ATTCTTTGCG 
GCTACTTCCA TTGTCCTCTT TCTCAACAAG AAGGACCTCT TTGAGGAAAA AATCAAGAAA 
GTCCATCTCA GCATTTGTTT TCCAGAGTAT GATGGTAACA ACTCCTATGA TGATGCGGGG 
AATTACATAA AGAGCCAGTT CCTTGACCTC AATATGCGAA AAGATGTCAA AGAAATCTAC 
AGTCACATGA CCTGTGCTAC AGATACACAG AATGTCAAAT TTGTGTTTGA TGCAGTTACA 
GATATTATCA TCAAAGAAAA CCTCAAGGAC TGCGGCCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular genetics of achromatopsia in Newfoundland reveal genetic heterogeneity, founder effects and the first cases of Jalili syndrome in North America.
Ophthalmic genetics34(3)119-29(2013 Sep)
Doucette L,Green J,Black C,Schwartzentruber J,Johnson GJ,Galutira D,Young TL

Genome-wide meta-analysis identifies 11 new loci for anthropometric traits and provides insights into genetic architecture.
Nature genetics45(5)501-12(2013 May)
Berndt SI,Gustafsson S,M?gi R,Ganna A,Wheeler E,Feitosa MF,Justice AE,Monda KL,Croteau-Chonka DC,Day FR,Esko T,Fall T,Ferreira T,Gentilini D,Jackson AU,Luan J,Randall JC,Vedantam S,Willer CJ,Winkler TW,Wood AR,Workalemahu T,Hu YJ,Lee SH,Liang L,Lin DY,Min JL,Neale BM,Thorleifsson G,Yang J,Albrecht E,Amin N,Bragg-Gresham JL,Cadby G,den Heijer M,Eklund N,Fischer K,Goel A,Hottenga JJ,Huffman JE,Jarick I,Johansson ?,Johnson T,Kanoni S,Kleber ME,K?nig IR,Kristiansson K,Kutalik Z,Lamina C,Lecoeur C,Li G,Mangino M,McArdle WL,Medina-Gomez C,M?ller-Nurasyid M,Ngwa JS,Nolte IM,Paternoster L,Pechlivanis S,Perola M,Peters MJ,Preuss M,Rose LM,Shi J,Shungin D,Smith AV,Strawbridge RJ,Surakka I,Teumer A,Trip MD,Tyrer J,Van Vliet-Ostaptchouk JV,Vandenput L,Waite LL,Zhao JH,Absher D,Asselbergs FW,Atalay M,Attwood AP,Balmforth AJ,Basart H,Beilby J,Bonnycastle LL,Brambilla P,Bruinenberg M,Campbell H,Chasman DI,Chines PS,Collins FS,Connell JM,Cookson WO,de Faire U,de Vegt F,Dei M,Dimitriou M,Edkins S,Estrada K,Evans DM,Farrall M,Ferrario MM,Ferri?res J,Franke L,Frau F,Gejman PV,Grallert H,Gr?nberg H,Gudnason V,Hall AS,Hall P,Hartikainen AL,Hayward C,Heard-Costa NL,Heath AC,Hebebrand J,Homuth G,Hu FB,Hunt SE,Hypp?nen E,Iribarren C,Jacobs KB,Jansson JO,Jula A,K?h?nen M,Kathiresan S,Kee F,Khaw KT,Kivim?ki M,Koenig W,Kraja AT,Kumari M,Kuulasmaa K,Kuusisto J,Laitinen JH,Lakka TA,Langenberg C,Launer LJ,Lind L,Lindstr?m J,Liu J,Liuzzi A,Lokki ML,Lorentzon M,Madden PA,Magnusson PK,Manunta P,Marek D,M?rz W,Mateo Leach I,McKnight B,Medland SE,Mihailov E,Milani L,Montgomery GW,Mooser V,M?hleisen TW,Munroe PB,Musk AW,Narisu N,Navis G,Nicholson G,Nohr EA,Ong KK,Oostra BA,Palmer CN,Palotie A,Peden JF,Pedersen N,Peters A,Polasek O,Pouta A,Pramstaller PP,Prokopenko I,P?tter C,Radhakrishnan A,Raitakari O,Rendon A,Rivadeneira F,Rudan I,Saaristo TE,Sambrook JG,Sanders AR,Sanna S,Saramies J,Schipf S,Schreiber S,Schunkert H,Shin SY,Signorini S,Sinisalo J,Skrobek B,Soranzo N,Stan??kov? A,Stark K,Stephens JC,Stirrups K,Stolk RP,Stumvoll M,Swift AJ,Theodoraki EV,Thorand B,Tregouet DA,Tremoli E,Van der Klauw MM,van Meurs JB,Vermeulen SH,Viikari J,Virtamo J,Vitart V,Waeber G,Wang Z,Wid?n E,Wild SH,Willemsen G,Winkelmann BR,Witteman JC,Wolffenbuttel BH,Wong A,Wright AF,Zillikens MC,Amouyel P,Boehm BO,Boerwinkle E,Boomsma DI,Caulfield MJ,Chanock SJ,Cupples LA,Cusi D,Dedoussis GV,Erdmann J,Eriksson JG,Franks PW,Froguel P,Gieger C,Gyllensten U,Hamsten A,Harris TB,Hengstenberg C,Hicks AA,Hingorani A,Hinney A,Hofman A,Hovingh KG,Hveem K,Illig T,Jarvelin MR,J?ckel KH,Keinanen-Kiukaanniemi SM,Kiemeney LA,Kuh D,Laakso M,Lehtim?ki T,Levinson DF,Martin NG,Metspalu A,Morris AD,Nieminen MS,Nj?lstad I,Ohlsson C,Oldehinkel AJ,Ouwehand WH,Palmer LJ,Penninx B,Power C,Province MA,Psaty BM,Qi L,Rauramaa R,Ridker PM,Ripatti S,Salomaa V,Samani NJ,Snieder H,S?rensen TI,Spector TD,Stefansson K,T?njes A,Tuomilehto J,Uitterlinden AG,Uusitupa M,van der Harst P,Vollenweider P,Wallaschofski H,Wareham NJ,Watkins H,Wichmann HE,Wilson JF,Abecasis GR,Assimes TL,Barroso I,Boehnke M,Borecki IB,Deloukas P,Fox CS,Frayling T,Groop LC,Haritunian T,Heid IM,Hunter D,Kaplan RC,Karpe F,Moffatt MF,Mohlke KL,O'Connell JR,Pawitan Y,Schadt EE,Schlessinger D,Steinthorsdottir V,Strachan DP,Thorsteinsdottir U,van Duijn CM,Visscher PM,Di Blasio AM,Hirschhorn JN,Lindgren CM,Morris AP,Meyre D,Scherag A,McCarthy MI,Speliotes EK,North KE,Loos RJ,Ingelsson E

Clinical utility gene card for: achromatopsia.
European journal of human genetics : EJHG19(6)(2011 Jun)
Kohl S,Hamel CP

Clinical and genetic investigation of a large Tunisian family with complete achromatopsia: identification of a new nonsense mutation in GNAT2 gene.
Journal of human genetics56(1)22-8(2011 Jan)
Ouechtati F,Merdassi A,Bouyacoub Y,Largueche L,Derouiche K,Ouragini H,Nouira S,Tiab L,Baklouti K,Rebai A,Schorderet DF,Munier FL,Zografos L,Abdelhak S,El Matri L

Replacing the rod with the cone transducin subunit decreases sensitivity and accelerates response decay.
The Journal of physiology588(Pt 17)3231-41(2010 Sep)
Chen CK,Woodruff ML,Chen FS,Shim H,Cilluffo MC,Fain GL

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GNAT2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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