GNAT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GNAT1
Entrez Gene ID	2779
Full Name	G protein subunit alpha transducin 1
Synonyms	CSNB1G,CSNBAD3,GBT1,GNATR
General protein information	Preferred Names G protein subunit alpha transducin 1 Names guanine nucleotide-binding protein G(t) subunit alpha-1 guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1 guanine nucleotide-binding protein G(T), alpha-1 subunit rod-type transducin alpha subunit transducin alpha-1 chain transducin, rod-specific
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.31
Summary	Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009].
Disorder MIM:	139330
Disorder Html:	Night blindness, congenital stationary, autosomal dominant 3, 610444 (3); ?Night blindness, congenital stationary, type 1G, 616389 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_144499.2	NP_653082.1	guanine nucleotide-binding protein G(t) subunit alpha-1
NM_000172.3	NP_000163.2	guanine nucleotide-binding protein G(t) subunit alpha-1
NM_000172.4	NP_000163.2	guanine nucleotide-binding protein G(t) subunit alpha-1
NM_144499.3	NP_653082.1	guanine nucleotide-binding protein G(t) subunit alpha-1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GNAT1	XP_001167971.1
Canis lupus familiaris (dog)	GNAT1	NP_001003068.1
Mus musculus (house mouse)	Gnat1	NP_032166.1
Rattus norvegicus (Norway rat)	Gnat1	NP_001102250.2
Gallus gallus (chicken)	GNAT1	NP_990022.1
Xenopus tropicalis (tropical clawed frog)	gnat1	NP_001096278.1
Homo sapiens (human)	GNAT1	NP_653082.1
	GNAT1	XP_001103202.1
Bos taurus (cattle)	GNAT1	NP_851365.1
Danio rerio (zebrafish)	gnat1	NP_571943.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel homozygous truncating GNAT1 mutation implicated in retinal degeneration.
Carrigan M, Duignan E, Humphries P, Palfi A, Kenna PF, Farrar GJ
The British journal of ophthalmology100(4)495-500(2016 Apr)

Screening of candidate tumor-suppressor genes in 3p21.3 and investigation of the methylation of gene promoters in oral squamous cell carcinoma.
Wang K, Ling T, Wu H, Zhang J
Oncology reports29(3)1175-82(2013 Mar)

GNAT1 associated with autosomal recessive congenital stationary night blindness.
Naeem MA, Chavali VR, Ali S, Iqbal M, Riazuddin S, Khan SN, Husnain T, Sieving PA, Ayyagari R, Riazuddin S, Hejtmancik JF, Riazuddin SA
Investigative ophthalmology & visual science53(3)1353-61(2012 Mar)

Simultaneous mutation detection in 90 retinal disease genes in multiple patients using a custom-designed 300-kb retinal resequencing chip.
Booij JC, Bakker A, Kulumbetova J, Moutaoukil Y, Smeets B, Verheij J, Kroes HY, Klaver CC, van Schooneveld M, Bergen AA, Florijn RJ
Ophthalmology118(1)160-167.e1-3(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data suggest that some truncating GNAT1 variants can indeed cause a recessive, mild, late-onset retinal degeneration in human beings rather than just stationary night-blindness as reported previously.
Title: A novel homozygous truncating GNAT1 mutation implicated in retinal degeneration.

Three candidate tumor-suppressor genes, SEMA3B, AXUD1 and GNAT1 may be involved in oral squamous cell carcinoma.
Title: Screening of candidate tumor-suppressor genes in 3p21.3 and investigation of the methylation of gene promoters in oral squamous cell carcinoma.

These data suggest that a homozygous missense mutation in GNAT1 is associated with autosomal recessive stationary night blindness.
Title: GNAT1 associated with autosomal recessive congenital stationary night blindness.

Observational study of genetic testing. (HuGE Navigator)
Title: Simultaneous mutation detection in 90 retinal disease genes in multiple patients using a custom-designed 300-kb retinal resequencing chip.

Expression of GNAT1 gene is down-regulated or absent in nasopharyngeal carcinoma tissues.
Title: [Expression, loss of heterozygosity, and methylation of GNAT1 gene in nasopharyngeal carcinoma].

The following GNAT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNAT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15872	NM_000172.4 Latest version!	Homo sapiens G protein subunit alpha transducin 1 (GNAT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$202.30 $289.00
OHu15872	NM_144499.3 Latest version!	Homo sapiens G protein subunit alpha transducin 1 (GNAT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$202.30 $289.00
View Old Accession Versions >>
OHu15872	NM_000172.3	Homo sapiens G protein subunit alpha transducin 1 (GNAT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$202.30 $289.00
OHu15872	NM_144499.2	Homo sapiens G protein subunit alpha transducin 1 (GNAT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$202.30 $289.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNAT1 ( NM_144499.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_653082.1 Homo sapiens G protein subunit alpha transducin 1 (GNAT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15872
Clone ID Related Accession (Same CDS sequence)	NM_144499.2 , NM_000172.3 , NM_000172.4 , NM_144499.3
Accession Version	NM_144499.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(t) subunit alpha-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC000063.1, BX953950.1, BC095505.1, BQ637667.1, AC002077.1 and BU727496.1. This sequence is a reference standard in the RefSeqGene project. On Feb 19, 2009 this sequence version replaced NM_144499.1. Transcript Variant: This variant (1) represents the longer transcript variant. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: X15088.1, BC095505.1 [ECO:0000331] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGGCTG GGGCCAGTGC TGAGGAGAAG CACTCCAGGG AGCTGGAAAA GAAGCTGAAA 
GAGGACGCTG AGAAGGATGC TCGAACCGTG AAGCTGCTGC TTCTGGGTGC CGGTGAGTCC 
GGGAAGAGCA CCATCGTCAA GCAGATGAAG ATTATCCACC AGGACGGGTA CTCGCTGGAA 
GAGTGCCTCG AGTTTATCGC CATCATCTAC GGCAACACGT TGCAGTCCAT CCTGGCCATC 
GTACGCGCCA TGACCACACT CAACATCCAG TACGGAGACT CTGCACGCCA GGACGACGCC 
CGGAAGCTGA TGCACATGGC AGACACTATC GAGGAGGGCA CGATGCCCAA GGAGATGTCG 
GACATCATCC AGCGGCTGTG GAAGGACTCC GGTATCCAGG CCTGTTTTGA GCGCGCCTCG 
GAGTACCAGC TCAACGACTC GGCGGGCTAC TACCTCTCCG ACCTGGAGCG CCTGGTAACC 
CCGGGCTACG TGCCCACCGA GCAGGACGTG CTGCGCTCGC GAGTCAAGAC CACTGGCATC 
ATCGAGACGC AGTTCTCCTT CAAGGATCTC AACTTCCGGA TGTTCGATGT GGGCGGGCAG 
CGCTCGGAGC GCAAGAAGTG GATCCACTGC TTCGAGGGCG TGACCTGCAT CATCTTCATC 
GCGGCGCTGA GCGCCTACGA CATGGTGCTA GTGGAGGACG ACGAAGTGAA CCGCATGCAC 
GAGAGCCTGC ACCTGTTCAA CAGCATCTGC AACCACCGCT ACTTCGCCAC GACGTCCATC 
GTGCTCTTCC TTAACAAGAA GGACGTCTTC TTCGAGAAGA TCAAGAAGGC GCACCTCAGC 
ATCTGTTTCC CGGACTACGA TGGACCCAAC ACCTACGAGG ACGCCGGCAA CTACATCAAG 
GTGCAGTTCC TCGAGCTCAA CATGCGGCGC GACGTGAAGG AGATCTATTC CCACATGACG 
TGCGCCACCG ACACGCAGAA CGTCAAATTT GTCTTCGACG CTGTCACCGA CATCATCATC 
AAGGAGAACC TCAAAGACTG TGGCCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653082.1
CDS	117..1169
Translation	MGAGASAEEKHSRELEKKLKEDAEKDARTVKLLLLGAGESGKSTIVKQMKIIHQDGYSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFERASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFFEKIKKAHLSICFPDYDGPNTYEDAGNYIKVQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	NM_144499.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit alpha transducin 1 (GNAT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144499.2

ORF Insert Sequence:

ATGGGGGCTG GGGCCAGTGC TGAGGAGAAG CACTCCAGGG AGCTGGAAAA GAAGCTGAAA 
GAGGACGCTG AGAAGGATGC TCGAACCGTG AAGCTGCTGC TTCTGGGTGC CGGTGAGTCC 
GGGAAGAGCA CCATCGTCAA GCAGATGAAG ATTATCCACC AGGACGGGTA CTCGCTGGAA 
GAGTGCCTCG AGTTTATCGC CATCATCTAC GGCAACACGT TGCAGTCCAT CCTGGCCATC 
GTACGCGCCA TGACCACACT CAACATCCAG TACGGAGACT CTGCACGCCA GGACGACGCC 
CGGAAGCTGA TGCACATGGC AGACACTATC GAGGAGGGCA CGATGCCCAA GGAGATGTCG 
GACATCATCC AGCGGCTGTG GAAGGACTCC GGTATCCAGG CCTGTTTTGA GCGCGCCTCG 
GAGTACCAGC TCAACGACTC GGCGGGCTAC TACCTCTCCG ACCTGGAGCG CCTGGTAACC 
CCGGGCTACG TGCCCACCGA GCAGGACGTG CTGCGCTCGC GAGTCAAGAC CACTGGCATC 
ATCGAGACGC AGTTCTCCTT CAAGGATCTC AACTTCCGGA TGTTCGATGT GGGCGGGCAG 
CGCTCGGAGC GCAAGAAGTG GATCCACTGC TTCGAGGGCG TGACCTGCAT CATCTTCATC 
GCGGCGCTGA GCGCCTACGA CATGGTGCTA GTGGAGGACG ACGAAGTGAA CCGCATGCAC 
GAGAGCCTGC ACCTGTTCAA CAGCATCTGC AACCACCGCT ACTTCGCCAC GACGTCCATC 
GTGCTCTTCC TTAACAAGAA GGACGTCTTC TTCGAGAAGA TCAAGAAGGC GCACCTCAGC 
ATCTGTTTCC CGGACTACGA TGGACCCAAC ACCTACGAGG ACGCCGGCAA CTACATCAAG 
GTGCAGTTCC TCGAGCTCAA CATGCGGCGC GACGTGAAGG AGATCTATTC CCACATGACG 
TGCGCCACCG ACACGCAGAA CGTCAAATTT GTCTTCGACG CTGTCACCGA CATCATCATC 
AAGGAGAACC TCAAAGACTG TGGCCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAT1 ( NM_000172.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000163.2 Homo sapiens G protein subunit alpha transducin 1 (GNAT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15872
Clone ID Related Accession (Same CDS sequence)	NM_144499.2 , NM_000172.3 , NM_000172.4 , NM_144499.3
Accession Version	NM_000172.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(t) subunit alpha-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC000063.1, BX953950.1, BC095505.1, BU728210.1, AC002077.1 and BU727496.1. On Feb 19, 2009 this sequence version replaced NM_000172.2. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: X15088.1, BC095505.1 [ECO:0000331] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGGCTG GGGCCAGTGC TGAGGAGAAG CACTCCAGGG AGCTGGAAAA GAAGCTGAAA 
GAGGACGCTG AGAAGGATGC TCGAACCGTG AAGCTGCTGC TTCTGGGTGC CGGTGAGTCC 
GGGAAGAGCA CCATCGTCAA GCAGATGAAG ATTATCCACC AGGACGGGTA CTCGCTGGAA 
GAGTGCCTCG AGTTTATCGC CATCATCTAC GGCAACACGT TGCAGTCCAT CCTGGCCATC 
GTACGCGCCA TGACCACACT CAACATCCAG TACGGAGACT CTGCACGCCA GGACGACGCC 
CGGAAGCTGA TGCACATGGC AGACACTATC GAGGAGGGCA CGATGCCCAA GGAGATGTCG 
GACATCATCC AGCGGCTGTG GAAGGACTCC GGTATCCAGG CCTGTTTTGA GCGCGCCTCG 
GAGTACCAGC TCAACGACTC GGCGGGCTAC TACCTCTCCG ACCTGGAGCG CCTGGTAACC 
CCGGGCTACG TGCCCACCGA GCAGGACGTG CTGCGCTCGC GAGTCAAGAC CACTGGCATC 
ATCGAGACGC AGTTCTCCTT CAAGGATCTC AACTTCCGGA TGTTCGATGT GGGCGGGCAG 
CGCTCGGAGC GCAAGAAGTG GATCCACTGC TTCGAGGGCG TGACCTGCAT CATCTTCATC 
GCGGCGCTGA GCGCCTACGA CATGGTGCTA GTGGAGGACG ACGAAGTGAA CCGCATGCAC 
GAGAGCCTGC ACCTGTTCAA CAGCATCTGC AACCACCGCT ACTTCGCCAC GACGTCCATC 
GTGCTCTTCC TTAACAAGAA GGACGTCTTC TTCGAGAAGA TCAAGAAGGC GCACCTCAGC 
ATCTGTTTCC CGGACTACGA TGGACCCAAC ACCTACGAGG ACGCCGGCAA CTACATCAAG 
GTGCAGTTCC TCGAGCTCAA CATGCGGCGC GACGTGAAGG AGATCTATTC CCACATGACG 
TGCGCCACCG ACACGCAGAA CGTCAAATTT GTCTTCGACG CTGTCACCGA CATCATCATC 
AAGGAGAACC TCAAAGACTG TGGCCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000163.2
CDS	117..1169
Translation	MGAGASAEEKHSRELEKKLKEDAEKDARTVKLLLLGAGESGKSTIVKQMKIIHQDGYSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFERASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFFEKIKKAHLSICFPDYDGPNTYEDAGNYIKVQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	NM_000172.3
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit alpha transducin 1 (GNAT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000172.3

ORF Insert Sequence:

ATGGGGGCTG GGGCCAGTGC TGAGGAGAAG CACTCCAGGG AGCTGGAAAA GAAGCTGAAA 
GAGGACGCTG AGAAGGATGC TCGAACCGTG AAGCTGCTGC TTCTGGGTGC CGGTGAGTCC 
GGGAAGAGCA CCATCGTCAA GCAGATGAAG ATTATCCACC AGGACGGGTA CTCGCTGGAA 
GAGTGCCTCG AGTTTATCGC CATCATCTAC GGCAACACGT TGCAGTCCAT CCTGGCCATC 
GTACGCGCCA TGACCACACT CAACATCCAG TACGGAGACT CTGCACGCCA GGACGACGCC 
CGGAAGCTGA TGCACATGGC AGACACTATC GAGGAGGGCA CGATGCCCAA GGAGATGTCG 
GACATCATCC AGCGGCTGTG GAAGGACTCC GGTATCCAGG CCTGTTTTGA GCGCGCCTCG 
GAGTACCAGC TCAACGACTC GGCGGGCTAC TACCTCTCCG ACCTGGAGCG CCTGGTAACC 
CCGGGCTACG TGCCCACCGA GCAGGACGTG CTGCGCTCGC GAGTCAAGAC CACTGGCATC 
ATCGAGACGC AGTTCTCCTT CAAGGATCTC AACTTCCGGA TGTTCGATGT GGGCGGGCAG 
CGCTCGGAGC GCAAGAAGTG GATCCACTGC TTCGAGGGCG TGACCTGCAT CATCTTCATC 
GCGGCGCTGA GCGCCTACGA CATGGTGCTA GTGGAGGACG ACGAAGTGAA CCGCATGCAC 
GAGAGCCTGC ACCTGTTCAA CAGCATCTGC AACCACCGCT ACTTCGCCAC GACGTCCATC 
GTGCTCTTCC TTAACAAGAA GGACGTCTTC TTCGAGAAGA TCAAGAAGGC GCACCTCAGC 
ATCTGTTTCC CGGACTACGA TGGACCCAAC ACCTACGAGG ACGCCGGCAA CTACATCAAG 
GTGCAGTTCC TCGAGCTCAA CATGCGGCGC GACGTGAAGG AGATCTATTC CCACATGACG 
TGCGCCACCG ACACGCAGAA CGTCAAATTT GTCTTCGACG CTGTCACCGA CATCATCATC 
AAGGAGAACC TCAAAGACTG TGGCCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAT1 ( NM_000172.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000163.2 Homo sapiens G protein subunit alpha transducin 1 (GNAT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15872
Clone ID Related Accession (Same CDS sequence)	NM_144499.2 , NM_000172.3 , NM_000172.4 , NM_144499.3
Accession Version	NM_000172.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(t) subunit alpha-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC000063.1, BX953950.1, BC095505.1, BU728210.1, AC002077.1 and BU727496.1. On Jun 1, 2019 this sequence version replaced NM_000172.3. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC095505.1, AF493908.1 [ECO:0000331] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGGCTG GGGCCAGTGC TGAGGAGAAG CACTCCAGGG AGCTGGAAAA GAAGCTGAAA 
GAGGACGCTG AGAAGGATGC TCGAACCGTG AAGCTGCTGC TTCTGGGTGC CGGTGAGTCC 
GGGAAGAGCA CCATCGTCAA GCAGATGAAG ATTATCCACC AGGACGGGTA CTCGCTGGAA 
GAGTGCCTCG AGTTTATCGC CATCATCTAC GGCAACACGT TGCAGTCCAT CCTGGCCATC 
GTACGCGCCA TGACCACACT CAACATCCAG TACGGAGACT CTGCACGCCA GGACGACGCC 
CGGAAGCTGA TGCACATGGC AGACACTATC GAGGAGGGCA CGATGCCCAA GGAGATGTCG 
GACATCATCC AGCGGCTGTG GAAGGACTCC GGTATCCAGG CCTGTTTTGA GCGCGCCTCG 
GAGTACCAGC TCAACGACTC GGCGGGCTAC TACCTCTCCG ACCTGGAGCG CCTGGTAACC 
CCGGGCTACG TGCCCACCGA GCAGGACGTG CTGCGCTCGC GAGTCAAGAC CACTGGCATC 
ATCGAGACGC AGTTCTCCTT CAAGGATCTC AACTTCCGGA TGTTCGATGT GGGCGGGCAG 
CGCTCGGAGC GCAAGAAGTG GATCCACTGC TTCGAGGGCG TGACCTGCAT CATCTTCATC 
GCGGCGCTGA GCGCCTACGA CATGGTGCTA GTGGAGGACG ACGAAGTGAA CCGCATGCAC 
GAGAGCCTGC ACCTGTTCAA CAGCATCTGC AACCACCGCT ACTTCGCCAC GACGTCCATC 
GTGCTCTTCC TTAACAAGAA GGACGTCTTC TTCGAGAAGA TCAAGAAGGC GCACCTCAGC 
ATCTGTTTCC CGGACTACGA TGGACCCAAC ACCTACGAGG ACGCCGGCAA CTACATCAAG 
GTGCAGTTCC TCGAGCTCAA CATGCGGCGC GACGTGAAGG AGATCTATTC CCACATGACG 
TGCGCCACCG ACACGCAGAA CGTCAAATTT GTCTTCGACG CTGTCACCGA CATCATCATC 
AAGGAGAACC TCAAAGACTG TGGCCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000163.2
CDS	117..1169
Translation	MGAGASAEEKHSRELEKKLKEDAEKDARTVKLLLLGAGESGKSTIVKQMKIIHQDGYSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFERASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFFEKIKKAHLSICFPDYDGPNTYEDAGNYIKVQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	NM_000172.4
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit alpha transducin 1 (GNAT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000172.4

ORF Insert Sequence:

ATGGGGGCTG GGGCCAGTGC TGAGGAGAAG CACTCCAGGG AGCTGGAAAA GAAGCTGAAA 
GAGGACGCTG AGAAGGATGC TCGAACCGTG AAGCTGCTGC TTCTGGGTGC CGGTGAGTCC 
GGGAAGAGCA CCATCGTCAA GCAGATGAAG ATTATCCACC AGGACGGGTA CTCGCTGGAA 
GAGTGCCTCG AGTTTATCGC CATCATCTAC GGCAACACGT TGCAGTCCAT CCTGGCCATC 
GTACGCGCCA TGACCACACT CAACATCCAG TACGGAGACT CTGCACGCCA GGACGACGCC 
CGGAAGCTGA TGCACATGGC AGACACTATC GAGGAGGGCA CGATGCCCAA GGAGATGTCG 
GACATCATCC AGCGGCTGTG GAAGGACTCC GGTATCCAGG CCTGTTTTGA GCGCGCCTCG 
GAGTACCAGC TCAACGACTC GGCGGGCTAC TACCTCTCCG ACCTGGAGCG CCTGGTAACC 
CCGGGCTACG TGCCCACCGA GCAGGACGTG CTGCGCTCGC GAGTCAAGAC CACTGGCATC 
ATCGAGACGC AGTTCTCCTT CAAGGATCTC AACTTCCGGA TGTTCGATGT GGGCGGGCAG 
CGCTCGGAGC GCAAGAAGTG GATCCACTGC TTCGAGGGCG TGACCTGCAT CATCTTCATC 
GCGGCGCTGA GCGCCTACGA CATGGTGCTA GTGGAGGACG ACGAAGTGAA CCGCATGCAC 
GAGAGCCTGC ACCTGTTCAA CAGCATCTGC AACCACCGCT ACTTCGCCAC GACGTCCATC 
GTGCTCTTCC TTAACAAGAA GGACGTCTTC TTCGAGAAGA TCAAGAAGGC GCACCTCAGC 
ATCTGTTTCC CGGACTACGA TGGACCCAAC ACCTACGAGG ACGCCGGCAA CTACATCAAG 
GTGCAGTTCC TCGAGCTCAA CATGCGGCGC GACGTGAAGG AGATCTATTC CCACATGACG 
TGCGCCACCG ACACGCAGAA CGTCAAATTT GTCTTCGACG CTGTCACCGA CATCATCATC 
AAGGAGAACC TCAAAGACTG TGGCCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAT1 ( NM_144499.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_653082.1 Homo sapiens G protein subunit alpha transducin 1 (GNAT1), transcript variant 1, mRNA.

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CloneID	OHu15872
Clone ID Related Accession (Same CDS sequence)	NM_144499.2 , NM_000172.3 , NM_000172.4 , NM_144499.3
Accession Version	NM_144499.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(t) subunit alpha-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC000063.1, BX953950.1, BC095505.1, BQ637667.1, AC002077.1 and BU727496.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_144499.2. Transcript Variant: This variant (1) represents the longer transcript variant. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC095505.1, AF493908.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000232461.8/ ENSP00000232461.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGGCTG GGGCCAGTGC TGAGGAGAAG CACTCCAGGG AGCTGGAAAA GAAGCTGAAA 
GAGGACGCTG AGAAGGATGC TCGAACCGTG AAGCTGCTGC TTCTGGGTGC CGGTGAGTCC 
GGGAAGAGCA CCATCGTCAA GCAGATGAAG ATTATCCACC AGGACGGGTA CTCGCTGGAA 
GAGTGCCTCG AGTTTATCGC CATCATCTAC GGCAACACGT TGCAGTCCAT CCTGGCCATC 
GTACGCGCCA TGACCACACT CAACATCCAG TACGGAGACT CTGCACGCCA GGACGACGCC 
CGGAAGCTGA TGCACATGGC AGACACTATC GAGGAGGGCA CGATGCCCAA GGAGATGTCG 
GACATCATCC AGCGGCTGTG GAAGGACTCC GGTATCCAGG CCTGTTTTGA GCGCGCCTCG 
GAGTACCAGC TCAACGACTC GGCGGGCTAC TACCTCTCCG ACCTGGAGCG CCTGGTAACC 
CCGGGCTACG TGCCCACCGA GCAGGACGTG CTGCGCTCGC GAGTCAAGAC CACTGGCATC 
ATCGAGACGC AGTTCTCCTT CAAGGATCTC AACTTCCGGA TGTTCGATGT GGGCGGGCAG 
CGCTCGGAGC GCAAGAAGTG GATCCACTGC TTCGAGGGCG TGACCTGCAT CATCTTCATC 
GCGGCGCTGA GCGCCTACGA CATGGTGCTA GTGGAGGACG ACGAAGTGAA CCGCATGCAC 
GAGAGCCTGC ACCTGTTCAA CAGCATCTGC AACCACCGCT ACTTCGCCAC GACGTCCATC 
GTGCTCTTCC TTAACAAGAA GGACGTCTTC TTCGAGAAGA TCAAGAAGGC GCACCTCAGC 
ATCTGTTTCC CGGACTACGA TGGACCCAAC ACCTACGAGG ACGCCGGCAA CTACATCAAG 
GTGCAGTTCC TCGAGCTCAA CATGCGGCGC GACGTGAAGG AGATCTATTC CCACATGACG 
TGCGCCACCG ACACGCAGAA CGTCAAATTT GTCTTCGACG CTGTCACCGA CATCATCATC 
AAGGAGAACC TCAAAGACTG TGGCCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653082.1
CDS	117..1169
Translation	MGAGASAEEKHSRELEKKLKEDAEKDARTVKLLLLGAGESGKSTIVKQMKIIHQDGYSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFERASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFFEKIKKAHLSICFPDYDGPNTYEDAGNYIKVQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	NM_144499.3
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit alpha transducin 1 (GNAT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144499.3

ORF Insert Sequence:

ATGGGGGCTG GGGCCAGTGC TGAGGAGAAG CACTCCAGGG AGCTGGAAAA GAAGCTGAAA 
GAGGACGCTG AGAAGGATGC TCGAACCGTG AAGCTGCTGC TTCTGGGTGC CGGTGAGTCC 
GGGAAGAGCA CCATCGTCAA GCAGATGAAG ATTATCCACC AGGACGGGTA CTCGCTGGAA 
GAGTGCCTCG AGTTTATCGC CATCATCTAC GGCAACACGT TGCAGTCCAT CCTGGCCATC 
GTACGCGCCA TGACCACACT CAACATCCAG TACGGAGACT CTGCACGCCA GGACGACGCC 
CGGAAGCTGA TGCACATGGC AGACACTATC GAGGAGGGCA CGATGCCCAA GGAGATGTCG 
GACATCATCC AGCGGCTGTG GAAGGACTCC GGTATCCAGG CCTGTTTTGA GCGCGCCTCG 
GAGTACCAGC TCAACGACTC GGCGGGCTAC TACCTCTCCG ACCTGGAGCG CCTGGTAACC 
CCGGGCTACG TGCCCACCGA GCAGGACGTG CTGCGCTCGC GAGTCAAGAC CACTGGCATC 
ATCGAGACGC AGTTCTCCTT CAAGGATCTC AACTTCCGGA TGTTCGATGT GGGCGGGCAG 
CGCTCGGAGC GCAAGAAGTG GATCCACTGC TTCGAGGGCG TGACCTGCAT CATCTTCATC 
GCGGCGCTGA GCGCCTACGA CATGGTGCTA GTGGAGGACG ACGAAGTGAA CCGCATGCAC 
GAGAGCCTGC ACCTGTTCAA CAGCATCTGC AACCACCGCT ACTTCGCCAC GACGTCCATC 
GTGCTCTTCC TTAACAAGAA GGACGTCTTC TTCGAGAAGA TCAAGAAGGC GCACCTCAGC 
ATCTGTTTCC CGGACTACGA TGGACCCAAC ACCTACGAGG ACGCCGGCAA CTACATCAAG 
GTGCAGTTCC TCGAGCTCAA CATGCGGCGC GACGTGAAGG AGATCTATTC CCACATGACG 
TGCGCCACCG ACACGCAGAA CGTCAAATTT GTCTTCGACG CTGTCACCGA CATCATCATC 
AAGGAGAACC TCAAAGACTG TGGCCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel homozygous truncating GNAT1 mutation implicated in retinal degeneration.
The British journal of ophthalmology100(4)495-500(2016 Apr)
Carrigan M,Duignan E,Humphries P,Palfi A,Kenna PF,Farrar GJ

Screening of candidate tumor-suppressor genes in 3p21.3 and investigation of the methylation of gene promoters in oral squamous cell carcinoma.
Oncology reports29(3)1175-82(2013 Mar)
Wang K,Ling T,Wu H,Zhang J

GNAT1 associated with autosomal recessive congenital stationary night blindness.
Investigative ophthalmology & visual science53(3)1353-61(2012 Mar)
Naeem MA,Chavali VR,Ali S,Iqbal M,Riazuddin S,Khan SN,Husnain T,Sieving PA,Ayyagari R,Riazuddin S,Hejtmancik JF,Riazuddin SA

Simultaneous mutation detection in 90 retinal disease genes in multiple patients using a custom-designed 300-kb retinal resequencing chip.
Ophthalmology118(1)160-167.e1-3(2011 Jan)
Booij JC,Bakker A,Kulumbetova J,Moutaoukil Y,Smeets B,Verheij J,Kroes HY,Klaver CC,van Schooneveld M,Bergen AA,Florijn RJ

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GNAT1 cDNA ORF clone, Homo sapiens(human)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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