PCLO cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCLO
Entrez Gene ID	27445
Full Name	piccolo presynaptic cytomatrix protein
Synonyms	ACZ,PCH3
General protein information	Preferred Names piccolo presynaptic cytomatrix protein Names protein piccolo aczonin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q21.11
Summary	The protein encoded by this gene is part of the presynaptic cytoskeletal matrix, which is involved in establishing active synaptic zones and in synaptic vesicle trafficking. Variations in this gene have been associated with bipolar disorder and major depressive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011].
Disorder MIM:	604918
Disorder Html:	?Pontocerebellar hypoplasia, type 3, 608027 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017012006.1	XP_016867495.1	protein piccolo isoform X1
NM_014510.3	NP_055325.2	protein piccolo isoform 2
NM_014510.2	NP_055325.2	protein piccolo isoform 2
NM_033026.5	NP_149015.2	protein piccolo isoform 1
NM_033026.6	NP_149015.2	protein piccolo isoform 1
XM_017012006.2	XP_016867495.1	protein piccolo isoform X1
XM_017012007.1	XP_016867496.1	protein piccolo isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:dkey-108d22.4	XP_005164728.1
Homo sapiens (human)	PCLO	NP_149015.2
Rattus norvegicus (Norway rat)	Pclo	NP_064483.1
Pan troglodytes (chimpanzee)	PCLO	XP_001160384.2
Mus musculus (house mouse)	Pclo	NP_036125.4
Gallus gallus (chicken)	PCLO	XP_001231455.2
	PCLO	XP_002808407.1
Bos taurus (cattle)	PCLO	XP_003586004.1
Canis lupus familiaris (dog)	PCLO	XP_005631078.1
Xenopus tropicalis (tropical clawed frog)	pclo	XP_002935303.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome-Wide Significance for PCLO as a Gene for Major Depressive Disorder.
Mbarek H, Milaneschi Y, Hottenga JJ, Ligthart L, de Geus EJC, Ehli EA, Willemsen G, Davies GE, Smit JH, Boomsma DI, Penninx BWJH
Twin research and human genetics : the official journal of the International Society for Twin Studies20(4)267-270(2017 Aug)

Piccolo mediates EGFR signaling and acts as a prognostic biomarker in esophageal squamous cell carcinoma.
Zhang W, Hong R, Xue L, Ou Y, Liu X, Zhao Z, Xiao W, Dong D, Dong L, Fu M, Ma L, Lu N, Chen H, Song Y, Zhan Q
Oncogene36(27)3890-3902(2017 Jul)

A 7q21.11 microdeletion presenting with apparent intellectual disability without epilepsy.
Siddique A, Willoughby J, , McNeill A
American journal of medical genetics. Part A173(4)1128-1130(2017 04)

Quantitative proteomics reveals Piccolo as a candidate serological correlate of recovery from Guillain-Barr? syndrome.
Mateos-Hern?ndez L, Villar M, Doncel-P?rez E, Trevisan-Herraz M, Garc?a-Forcada ?, Ganuza FR, V?zquez J, de la Fuente J
Oncotarget7(46)74582-74591(2016 11)

GWAS-identified risk variants for major depressive disorder: Preliminary support for an association with late-life depressive symptoms and brain structural alterations.
Ryan J, Artero S, Carri?re I, Maller JJ, Meslin C, Ritchie K, Ancelin ML
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology26(1)113-25(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Piccolo contributes to tumor aggressiveness in esophageal squamous cell carcinoma, likely by stabilizing EGFR and promoting EGFR-dependent signaling.
Title: Piccolo mediates EGFR signaling and acts as a prognostic biomarker in esophageal squamous cell carcinoma.

we report an additional patient with the 7q21.11 deletion syndrome and provide evidence that haploinsufficiency for a single gene may not be the disease mechanism. In vitro studies of the interaction between PCLO and CACNA2D1 will be required to examine the hypothesis that combined haploinsufficiency for these two synaptic proteins results in neuronal dysfunction
Title: A 7q21.11 microdeletion presenting with apparent intellectual disability without epilepsy.

Results provide some support for the involvement of BICC1 and PCLO in late-life depressive disorders and preliminary evidence that these genetic variants may also influence brain structural volumes
Title: GWAS-identified risk variants for major depressive disorder: Preliminary support for an association with late-life depressive symptoms and brain structural alterations.

Study created and characterized a knock-in mouse model carrying the PCLO Ser to Ala missense variant rs2522833, which has shown suggestive association with major depressive disorder in human population studies
Title: Functional characterization of the PCLO p.Ser4814Ala variant associated with major depressive disorder reveals cellular but not behavioral differences.

A homozygous, nonsense PCLO mutation underlies the autosomal recessive neurodegenerative disorder pontocerebellar hypoplasia type III.
Title: Loss of PCLO function underlies pontocerebellar hypoplasia type III.

The following PCLO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCLO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24261	NM_014510.3 Latest version!	Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu24277	NM_033026.6 Latest version!	Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu97174	XM_017012006.2 Latest version!	Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu97175	XM_017012007.1 Latest version!	Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
View Old Accession Versions >>
OHu24261	NM_014510.2	Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu24277	NM_033026.5	Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu97174	XM_017012006.1	Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCLO ( NM_014510.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055325.2 Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu24261
Clone ID Related Accession (Same CDS sequence)	NM_014510.3 , NM_014510.2
Accession Version	NM_014510.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	protein piccolo isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093461.4, AC004903.2, AC004886.1, DN990884.1, AB011131.1, BG913494.1 and AC004082.1. On Jun 1, 2019 this sequence version replaced NM_014510.2. Transcript Variant: This variant (2) lacks several 3' terminal exons and differs at the 3' end compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA 
GCGGCTGGAG GAGGAGCTAG CGGGGCGGGG AGCCCCTCTC ACACCGCGAT CCCGGCCGGC 
ATGGAGGCGG ATTTGAGCCA GCTGAGCGAA GAGGAGAGGA GACAGATCGC CGCTGTCATG 
TCAAGGGCGC AGGGGCTGCC CAAGGGAAGC GTCCCCCCGG CCGCTGCGGA GTCGCCTTCC 
ATGCACAGGA AACAAGAGTT GGATAGTAGT CATCCTCCAA AGCAATCAGG AAGACCCCCG 
GACCCTGGGC GTCCAGCTCA ACCTGGTCTC AGTAAAAGTA GAACTACAGA CACTTTCAGG 
TCAGAGCAGA AATTGCCTGG GAGGAGTCCT TCCACTATTA GCTTGAAAGA ATCAAAGTCC 
AGAACTGATT TAAAGGAAGA GCACAAGTCT AGTATGATGC CTGGCTTCCT CTCAGAGGTT 
AACGCTTTAA GTGCTGTTTC CTCTGTTGTA AATAAATTCA ACCCTTTTGA TTTGATATCA 
GACTCTGAGG CATCCCAGGA AGAAACCACC AAGAAACAAA AAGTGGTTCA GAAGGAGCAA 
GGAAAACCTG AAGGAATCAT AAAACCTCCT TTACAACAAC AGCCACCCAA GCCGATTCCT 
AAGCAGCAAG GACCTGGTAG GGATCCGCTT CAGCAGGATG GCACTCCCAA ATCAATATCT 
TCTCAACAAC CAGAAAAAAT TAAATCACAA CCTCCAGGTA CAGGAAAGCC AATTCAGGGT 
CCTACCCAGA CTCCTCAGAC AGACCATGCA AAATTGCCAC TTCAACGAGA TGCATCCAGG 
CCTCAGACTA AACAGGCAGA CATAGTAAGG GGAGAATCAG TTAAACCCTC ACTGCCAAGC 
CCATCCAAAC CACCTATTCA GCAACCAACT CCTGGAAAAC CTCCAGCACA GCAGCCTGGA 
CATGAAAAAT CACAGCCTGG GCCTGCAAAG CCCCCAGCTC AGCCCTCAGG GCTAACAAAG 
CCATTGGCTC AACAACCAGG GACAGTGAAA CCCCCAGTCC AGCCACCAGG GACAACAAAG 
CCTCCAGCTC AGCCTCTTGG TCCTGCTAAG CCTCCAGCTC AGCAGACTGG GTCAGAGAAG 
CCTTCATCGG AGCAGCCTGG GCCAAAGGCT TTAGCTCAGC CTCCTGGAGT TGGAAAGACT 
CCAGCTCAAC AGCCAGGGCC AGCAAAGCCT CCAACCCAGC AGGTGGGGAC ACCAAAACCC 
CTAGCTCAAC AACCTGGGCT ACAGTCTCCA GCTAAGGCAC CTGGGCCTAC AAAGACTCCA 
GTTCAGCAGC CTGGGCCAGG AAAGATTCCA GCTCAACAGG CAGGACCCGG AAAGACTTCT 
GCCCAGCAGA CTGGCCCAAC AAAGCCTCCT TCACAACTGC CTGGCCCAGC AAAGCCCCCA 
CCTCAACAGC CTGGCCCAGC AAAGCCCCCA CCTCAACAGC CTGGCTCAGC AAAGCCCCCA 
TCTCAACAGC CTGGCTCAAC AAAACCCCCA CCTCAACAGC CTGGCCCAGC AAAGCCCTCA 
CCTCAACAGC CTGGCTCAAC AAAACCCCCA TCTCAACAGC CTGGCTCAGC AAAACCCTCA 
GCTCAACAGC CTAGCCCAGC AAAGCCCTCG GCTCAGCAAT CTACAAAACC AGTAAGCCAA 
ACAGGATCTG GAAAACCTCT GCAGCCACCA ACAGTGTCTC CATCTGCAAA ACAGCCTCCT 
TCACAAGGCC TCCCTAAAAC CATCTGTCCT CTTTGCAATA CCACTGAACT TCTGTTGCAT 
GTTCCAGAAA AGGCCAATTT TAACACATGC ACTGAGTGTC AAACCACTGT CTGTAGTCTC 
TGTGGTTTTA ATCCCAATCC TCATTTAACG GAGGTAAAAG AGTGGCTCTG TTTGAACTGT 
CAAATGAAAA GAGCTCTAGG CGGGGATCTG GCTCCAGTTC CGTCATCACC CCAGCCCAAA 
CTGAAGACTG CACCTGTTAC CACTACATCA GCAGTGAGCA AATCATCCCC ACAGCCACAG 
CAGACTTCCC CAAAGAAGGA TGCTGCACCA AAACAGGATC TCTCCAAGGC ACCTGAGCCT 
AAAAAGCCAC CACCACTAGT GAAACAACCA ACCCTTCATG GCTCTCCTTC AGCCAAGGCC 
AAGCAGCCCC CTGAGGCAGA TTCTTTGTCC AAGCCAGCCC CTCCCAAAGA ACCTTCTGTC 
CCATCTGAGC AGGACAAGGC CCCTGTTGCT GATGATAAAC CAAAGCAGCC CAAGATGGTA 
AAGCCAACCA CTGACCTTGT ATCTTCATCA TCAGCAACAA CAAAACCTGA TATTCCAAGC 
TCCAAAGTAC AGTCACAAGC TGAAGAGAAA ACAACCCCTC CTCTAAAAAC AGACTCTGCC 
AAACCCTCAC AGAGTTTTCC ACCAACAGGG GAAAAAGTCA GCCCATTTGA TTCTAAAGCC 
ATACCTCGAC CTGCATCAGA TTCAAAAATT ATTTCACATC CTGGTCCCAG TTCAGAGAGC 
AAAGGTCAAA AACAAGTTGA CCCCGTACAA AAGAAGGAAG AACCCAAGAA AGCACAAACC 
AAAATGAGTC CTAAACCAGA TGCCAAGCCA ATGCCAAAAG GGTCACCAAC ACCCCCTGGC 
CCACGACCTA CCGCTGGCCA AACTGTCCCC ACACCTCAAC AGTCCCCAAA GCCTCAGGAG 
CAGTCAAGGC GTTTCAGTCT GAATCTGGGA AGTATTACTG ATGCCCCCAA ATCACAGCCT 
ACAACTCCTC AAGAGACCGT GACTGGGAAA CTCTTTGGGT TTGGAGCATC AATCTTCAGC 
CAGGCATCAA ATTTAATTTC CACTGCAGGC CAACCTGGAC CTCATTCACA AAGTGGACCA 
GGGGCCCCAA TGAAACAAGC CCCTGCCCCT TCACAGCCAC CTACTTCACA AGGGCCACCC 
AAATCCACAG GTCAAGCACC ACCAGCACCT GCAAAAAGTA TACCTGTGAA AAAGGAAACA 
AAAGCCCCAG CAGCTGAAAA ATTAGAGCCC AAAGCTGAAC AAGCTCCAAC AGTAAAAAGA 
ACAGAAACAG AAAAAAAGCC ACCACCTATT AAGGATAGCA AATCTTTAAC AGCTGAGCCT 
CAAAAGGCTG TCCTTCCCAC AAAACTGGAG AAATCGCCCA AACCAGAATC AACCTGTCCT 
CTCTGCAAAA CTGAACTCAA CATAGGTTCT AAGGATCCTC CTAACTTCAA TACTTGCACT 
GAATGCAAGA ATCAAGTGTG TAATCTCTGT GGATTTAACC CTACACCACA TTTGACTGAG 
ATTCAAGAAT GGCTTTGTTT AAATTGCCAA ACCCAGAGAG CAATATCAGG ACAGCTTGGA 
GACATACGCA AAATGCCACC TGCACCATCA GGACCCAAAG CATCTCCTAT GCCTGTTCCT 
ACAGAATCAT CATCTCAGAA AACAGCAGTG CCTCCCCAAG TAAAATTAGT GAAAAAGCAA 
GAACAAGAAG TAAAAACGGA AGCTGAAAAA GTCATTCTGG AAAAAGTAAA GGAAACACTA 
TCAATGGAAA AAATTCCTCC TATGGTAACC ACAGATCAAA AACAAGAAGA GAGTAAACTA 
GAGAAAGACA AAGCTTCAGC TCTTCAAGAA AAAAAGCCAC TCCCTGAAGA AAAAAAACTA 
ATCCCTGAAG AAGAAAAGAT ACGTTCTGAA GAAAAAAAGC CACTCCTAGA AGAAAAAAAG 
CCAACCCCTG AAGACAAAAA GCTACTCCCA GAGGCAAAAA CATCAGCCCC AGAAGAACAG 
AAACATGACT TACTTAAATC TCAAGTACAA ATTGCTGAAG AAAAGCTTGA AGGCAGAGTG 
GCTCCAAAGA CAGTGCAAGA AGGGAAACAA CCACAGACCA AGATGGAAGG TTTACCATCT 
GGCACACCTC AGAGTTTACC TAAAGAAGAT GATAAGACAA CCAAAACAAT AAAAGAACAG 
CCACAGCCAC CATGCACAGC AAAACCTGAT CAGGTGGAAC CTGGGAAAGA AAAAACAGAA 
AAGGAAGATG ACAAATCAGA CACCTCAAGT TCTCAGCAGC CTAAAAGCCC CCAAGGTCTG 
AGCGACACGG GATATTCTTC CGATGGAATA TCAAGCTCAC TTGGTGAAAT TCCAAGTCTT 
ATTCCAACTG ATGAAAAGGA TATTCTCAAG GGACTCAAAA AGGACTCTTT TTCACAAGAA 
AGCAGCCCTT CCAGCCCCTC AGATTTGGCT AAGTTAGAAA GTACAGTCCT ATCTATTTTG 
GAAGCTCAAG CAAGTACACT TGCTGATGAA AAGTCAGAAA AGAAAACACA ACCCCATGAA 
GTTTCTCCTG AACAGCCTAA AGACCAAGAG AAAACTCAGA GTTTATCTGA AACCTTGGAA 
ATTACTATTT CAGAAGAGGA GATCAAAGAG AGTCAAGAAG AAAGGAAAGA CACTTTTAAA 
AAAGATAGCC AACAAGATAT TCCTTCCAGC AAGGACCATA AAGAGAAGTC TGAGTTTGTT 
GATGACATAA CTACTAGAAG AGAGCCTTAT GATTCAGTTG AAGAGAGTAG TGAAAGTGAA 
AACTCACCTG TTCCACAAAG AAAACGAAGA ACTAGTGTTG GCTCATCAAG CAGTGATGAG 
TATAAACAGG AAGACAGCCA AGGATCAGGG GAAGAGGAGG ACTTCATTCG AAAACAAATC 
ATAGAAATGA GTGCTGATGA AGATGCTTCA GGTTCTGAAG ATGATGAGTT CATCAGAAAC 
CAGCTCAAAG AGATTAGTAG CAGTACTGAG AGCCAGAAGA AGGAAGAAAC AAAGGGAAAA 
GGCAAAATAA CAGCAGGGAA ACACAGACGA CTGACTCGAA AAAGTAGCAC AAGCATTGAT 
GAAGATGCAG GAAGACGTCA CTCATGGCAT GATGAAGACG ATGAAGCATT TGATGAAAGT 
CCTGAACTTA AATACAGAGA AACTAAAAGT CAGGAAAGTG AAGAACTTGT AGTTACTGGA 
GGAGGAGGGC TACGCCGATT TAAAACAATT GAGCTCAACA GTACAATAGC AGATAAATAT 
TCTGCAGAGT CATCACAGAA AAAAACAAGT TTGTATTTTG ACGAAGAGCC AGAATTGGAA 
ATGGAAAGCC TGACAGACTC ACCTGAAGAT AGGTCAAGGG GAGAGGGATC TTCGAGTCTG 
CATGCTTCCA GCTTCACTCC TGGTACATCC CCTACATCAG TATCATCACT TGATGAGGAC 
AGTGACAGTA GCCCGAGTCA CAAAAAAGGA GAGAGCAAAC AGCAACGCAA AGCTCGGCAC 
AGACCACATG GCCCTCTTTT GCCTACTATT GAAGATTCTT CAGAGGAAGA AGAATTGAGA 
GAGGAAGAAG AATTATTAAA GGAGCAAGAA AAGCAGAGGG AAATAGAACA GCAACAAAGA 
AAGAGTTCTA GTAAAAAATC AAAGAAAGAC AAAGATGAAC TTCGAGCTCA GAGAAGAAGG 
GAAAGGCCAA AGACACCACC TAGTAATCTC TCTCCCATTG AAGATGCATC TCCGACAGAA 
GAGTTACGTC AGGCTGCAGA AATGGAGGAG CTCCATAGAT CTTCTTGTTC TGAATATTCA 
CCTAGCATAG AGTCAGACCC AGAAGGTTTT GAAATAAGCC CGGAAAAAAT AATAGAAGTA 
CAAAAAGTTT ATAAATTGCC CACAGCTGTT TCATTATACT CACCAACAGA TGAGCAATCT 
ATTATGCAGA AAGAAGGTAG CCAAAAGGCG TTAAAAAGTG CTGAGGAGAT GTATGAAGAA 
ATGATGCATA AAACACACAA ATACAAAGCT TTTCCAGCTG CAAATGAACG AGATGAAGTG 
TTTGAAAAAG AGCCTTTGTA TGGTGGGATG CTAATAGAGG ATTATATTTA TGAATCTTTA 
GTAGAAGACA CGTACAATGG ATCGGTAGAT GGCAGTCTGC TAACAAGGCA AGAAGAAGAA 
AATGGATTTA TGCAGCAGAA AGGAAGAGAG CAAAAGATAA GACTTTCAGA ACAGATTTAT 
GAAGATCCTA TGCAGAAAAT TACAGACCTC CAGAAAGAGT TTTATGAGTT AGAAAGCTTA 
CATTCTGTTG TGCCTCAGGA AGATATTGTT TCAAGCTCTT TTATCATCCC AGAAAGCCAT 
GAGATAGTGG ACCTGGGTAC TATGGTAACT TCTACAGAAG AAGAAAGGAA ACTACTAGAT 
GCTGATGCTG CCTATGAAGA ACTTATGAAG AGGCAACAGA TGCAATTAAC ACCTGGATCT 
AGCCCAACCC AGGCCCCCAT TGGTGAGGAT ATGACAGAGT CCACCATGGA CTTTGACAGA 
ATGCCAGATG CCTCTTTGAC ATCAAGTGTT CTCTCAGGAG CGTCTCTTAC AGATTCGACC 
AGCAGTGCAA CACTCTCTAT CCCAGATGTT AAAATAACCC AACATTTTTC AACAGAAGAA 
ATTGAGGATG AATATGTAAC CGATTATACA AGAGAAATTC AAGAGATAAT TGCCCATGAA 
TCGCTGATTT TGACCTACTC GGAGCCTTCA GAAAGTGCTA CATCTGTCCC ACCCTCTGAC 
ACACCTTCTC TCACATCATC TGTTTCTTCG GTCTGTACCA CAGATAGCTC TTCACCCATT 
ACTACCCTGG ATAGCATAAC CACAGTTTAT ACAGAGCCAG TGGACATGAT AACTAAATTT 
GAAGATTCTG AGGAAATTTC TTCATCAACT TATTTTCCAG GCAGCATTAT AGACTATCCA 
GAAGAAATAA GTGTATCTTT AGATCGGACT GCCCCACCAG ATGGTAGAGC TAGTGCTGAT 
CATATTGTTA TTTCCTTATC TGATATGGCA TCTTCTATCA TAGAATCTGT AGTACCTAAA 
CCTGAAGGGC CAGTTGCTGA CACTGTTTCT ACTGACTTAC TTATATCTGA AAAGGACCCA 
GTGAAGAAAG CCAAGAAGGA AACTGGGAAT GGAATCATTC TGGAAGTTTT GGAAGCTTAC 
AGAGATAAAA AGGAGTTGGA GGCCGAACGA ACAAAAAGTA GCTTATCCGA AACCGTGTTT 
GATCACCCAC CTTCTTCTGT AATAGCCCTT CCAATGAAAG AGCAGCTTTC AACTACATAC 
TTTACATCTG GAGAGACCTT TGGTCAGGAA AAACCTGCAT CTCAGTTACC ATCTGGCAGT 
CCTTCTGTTT CCTCTCTTCC AGCTAAACCT CGCCCATTCT TTAGAAGTTC TTCTTTGGAT 
ATATCAGCTC AACCTCCTCC CCCTCCTCCC CCTCCCCCTC CTCCTCCTCC TCCACCACCA 
CCCCCTCCTC CCCCACCACT TCCTCCACCA ACTTCACCTA AACCAACTAT TCTTCCTAAA 
AAAAAGTTAA CAGTTGCATC TCCAGTGACT ACAGCTACAC CTCTGTTTGA TGCTGTTACT 
ACTCTAGAGA CCACAGCTGT TCTGAGAAGT AATGGATTAC CTGTTACAAG AATATGTACT 
ACTGCACCTC CTCCTGTTCC TCCTAAGCCA TCTTCAATTC CATCTGGACT TGTATTTACC 
CACAGGCCTG AGCCAAGCAA ACCTCCAATC GCCCCCAAAC CAGTGATTCC TCAGCTTCCA 
ACAACTACAC AAAAACCAAC AGATATACAC CCCAAACCAA CAGGCCTATC TTTAACTTCA 
AGTATGACCT TAAATTTAGT GACTTCAGCA GATTATAAAT TGCCTTCCCC TACCTCCCCA 
CTTTCCCCAC ACTCCAACAA GTCTTCACCA AGATTTTCCA AATCCCTCAC AGAAACTTAT 
GTAGTTATTA CATTGCCATC TGAACCAGGG ACTCCAACAG ATTCTTCTGC TAGTCAAGCA 
ATTACCAGTT GGCCCTTGGG ATCACCCTCC AAAGATCTGG TTTCTGTTGA ACCTGTGTTT 
TCTGTAGTTC CTCCTGTGAC AGCTGTAGAA ATTCCAATTT CTTCAGAACA GACCTTCTAC 
ATCTCTGGAG CTTTACAGAC ATTTTCTGCT ACCCCTGTCA CAGCACCCTC TTCATTTCAA 
GCAGCTCCCA CATCAGTTAC ACAGTTTCTC ACTACTGAAG TTTCCAAGAC TGAGGTTTCA 
GCAACCAGAA GTACAGCTCC TAGTGTTGGT CTCAGCAGCA TTTCCATAAC AATTCCTCCA 
GAGCCTCTTG CTCTAGATAA CATACATTTA GAGAAGCCTC AGTATAAAGA AGATGGAAAA 
TTGCAACTTG TTGGTGATGT AATTGATTTG CGTACAGTAC CAAAGGTAGA AGTTAAAACA 
ACTGATAAAT GTATTGATCT TTCTGCTTCT ACAATGGATG TGAAAAGGCA GATCACAGCA 
AATGAAGTTT ATGGGAAACA AATTAGTGCT GTCCAACCCT CTATTATAAA TCTTAGTGTG 
ACATCATCAA TAGTGACTCC TGTATCTCTG GCCACTGAGA CAGTGACCTT TGTCACATGC 
ACAGCTAGTG CAAGTTACAC TACAGGCACA GAAAGCCTAG TGGGTGCAGA ACATGCAATG 
ACAACACCAC TCCAACTTAC AACATCAAAG CATGCTGAGC CCCCATACAG GATACCAAGT 
GACCAGGTCT TTCCTATAGC TAGGGAAGAA GCACCAATAA ACTTATCTCT AGGTACTCCA 
GCACATGCAG TGACATTGGC TATTACAAAA CCTGTCACTG TGCCTCCTGT TGGTGTCACA 
AATGGATGGA CTGATAGCAC CGTATCCCAG GGAATCACTG ATGGGGAAGT AGTGGATCTC 
AGTACAACCA AGTCTCACAG AACAGTCGTA ACAATGGATG AGTCTACTTC AAGTGTGATG 
ACCAAAATAA TAGAAGATGA AAAACCCGTT GATTTAACCG CAGGGAGAAG AGCTGTGTGC 
TGTGATGTGG TTTATAAATT ACCATTTGGA AGGAGCTGCA CAGCACAGCA GCCTGCAACT 
ACTCTTCCTG AGGATCGTTT TGGTTATAGG GATGACCACT ATCAGTATGA TCGATCAGGG 
CCATATGGTT ATAGAGGGAT TGGGGGAATG AAGCCTTCCA TGTCTGACAC AAATTTAGCA 
GAAGCTGGAC ATTTTTTCTA TAAAAGTAAG AATGCTTTTG ATTATTCTGA AGGAACTGAC 
ACAGCAGTAG ATCTGACTTC AGGGAGAGTT ACTACAGGTG AGGTAATGGA TTATTCAAGC 
AAGACTACAG GTCCATATCC AGAAACACGA CAAGTCATTT CAGGAGCTGG GATTAGTACC 
CCACAGTATT CCACAGCAAG AATGACACCA CCACCAGGAC CCCAGTATTG TGTGGGGAGT 
GTTTTGAGGT CATCTAATGG TGTTGTCTAT TCTTCAGTAG CAACTCCAAC ACCCTCTACA 
TTTGCTATCA CCACACAACC TGGCTCCATT TTCAGCACCA CAGTGAGGGA TTTGTCTGGT 
ATTCATACGG CTGATGCAGT GACTTCATTA CCTGCCATGC ACCATAGCCA GCCAATGCCT 
AGATCATATT TTATAACAAC AGGTGCATCT GAAACGGACA TTGCAGTAAC TGGTATTGAT 
ATCAGTGCCA GTTTGCAAAC TATTACTATG GAGTCTCTTA CTGCTGAGAC GATAGACTCT 
GTTCCCACTT TAACCACAGC ATCCGAAGTG TTTCCTGAAG TGGTGGGAGA TGAAAGTGCT 
CTTTTAATTG TCCCTGAAGA AGATAAACAA CAGCAGCAGC TAGACTTGGA GCGTGAGCTC 
CTGGAACTGG AGAAAATTAA GCAACAGCGC TTTGCTGAGG AATTGGAGTG GGAACGTCAG 
GAAATTCAAA GGTTCCGAGA ACAAGAAAAG ATCATGGTTC AGAAAAAGTT GGAGGAGCTG 
CAGTCTATGA AGCAACACCT TCTCTTTCAG CAAGAAGAAG AGCGGCAAGC CCAGTTCATG 
ATGAGGCAGG AGACGTTAGC TCAGCAACAG TTACAGCTTG AGCAGATCCA ACAGCTGCAA 
CAACAGCTTC ACCAGCAGCT GGAGGAGCAA AAGATTCGGC AGATCTACCA GTATAACTAT 
GACCCTTCTG GAACTGCTTC TCCACAAACC ACTACAGAGC AGGCAATTTT GGAAGGTCAG 
TATGCTGCTC TGGAAGGCAG TCAATTTTGG GCAACTGAAG ATGCAACCAC CACAGCTTCA 
GCTGTTGTGG CAATTGAAAT ACCACAAAGC CAAGGATGGT ACACCGTTCA GTCTGATGGT 
GTTACTCAGT ACATTGCCCC ACCTGGTATC CTGAGCACTG TTTCAGAAAT ACCTCTAACA 
GATGTTGTTG TGAAAGAGGA AAAACAACCC AAAAAGAGAA GTTCTGGAGC TAAAGTCCGA 
GGACAGTATG ATGACATGGG AGAAAATATG ACAGATGATC CCCGAAGTTT TAAAAAGATA 
GTGGACAGTG GTGTACAAAC GGATGACGAA GATGCCACAG ATCGGAGCTA TGTGAGTAGG 
AGAAGGAGAA CTAAAAAGAG TGTGGATACA AGCGTCCAAA CTGATGATGA AGATCAGGAT 
GAGTGGGATA TGCCTACTAG ATCAAGGAGG AAAGCTCGTG TAGGGAAATA TGGTGACAGC 
ATGACAGAGG CTGACAAGAC CAAACCCCTT TCCAAAGTCT CCAGCATAGC AGTTCAAACG 
GTAGCAGAGA TATCTGTGCA AACTGAACCA GTTGGAACCA TAAGAACACC CTCCATACGG 
GCACGAGTGG ATGCCAAGGT AGAAATAATT AAACACATTT CAGCACCTGA AAAGACTTAC 
AAAGGGGGCA GTTTAGGATG TCAAACAGAA GCAGATTCAG ACACACAAAG TCCTCAATAT 
CTGAGTGCCA CATCTCCACC CAAAGACAAG AAACGCCCAA CACCTTTAGA GATTGGTTAT 
TCATCTCACC TCCGGGCAGA TTCCACAGTA CAGCTGGCTC CTTCCCCACC CAAATCCCCC 
AAAGTCCTTT ACTCACCCAT CTCACCACTT TCACCAGGCA AAGCCTTAGA ATCAGCCTTT 
GTACCTTATG AAAAACCCCT CCCTGATGAT ATAAGTCCAC AGAAAGTACT GCATCCAGAT 
ATGGCTAAAG TTCCCCCAGC AAGTCCTAAG ACAGCCAAGA TGATGCAGCG TTCTATGTCT 
GACCCCAAGC CTCTGAGTCC AACAGCAGAC GAAAGTTCCA GGGCTCCTTT TCAGTATACC 
GAGGGCTATA CGACTAAAGG TTCTCAAACC ATGACATCCT CTGGAGCCCA GAAAAAAGTT 
AAAAGAACTC TGCCAAATCC ACCTCCTGAG GAGATTTCCA CAGGAACTCA ATCCACATTC 
AGCACAATGG GCACAGTTTC CAGGAGAAGG ATCTGCAGAA CCAACACAAT GGCACGAGCC 
AAGATTCTCC AGGACATAGA CAGAGAGCTT GATCTTGTGG AAAGGGAGTC TGCAAAACTT 
CGAAAGAAAC AAGCAGAGCT TGATGAAGAA GAAAAGGAGA TTGATGCTAA GCTACGATAC 
CTGGAAATGG GAATTAACAG GAGGAAAGAG GCCCTATTAA AGGAGAGAGA AAAGAGAGAA 
CGAGCCTACC TCCAGGGAGT AGCTGAGGAT CGTGATTACA TGTCTGACAG TGAAGTGAGT 
AGCACAAGAC CAACCCGAAT AGAAAGTCAG CATGGCATTG AGCGACCAAG AACTGCTCCC 
CAAACTGAAT TCAGCCAGTT TATACCACCA CAAACCCAAA CAGAATCTCA ACTAGTTCCT 
CCGACAAGTC CTTACACACA ATACCAGTAC TCTTCCCCTG CTCTTCCTAC CCAAGCACCC 
ACCTCATACA CTCAACAGTC TCATTTTGAG CAACAAACTT TGTACCATCA GCAAGTTTCA 
CCTTATCAGA CTCAGCCAAC ATTCCAAGCT GTGGCAACAA TGTCCTTCAC ACCTCAAGTT 
CAACCTACAC CAACCCCACA GCCTTCTTAT CAGTTACCTT CACAGATGAT GGTGATACAA 
CAGAAGCCAC GGCAAACTAC ATTATATTTG GAGCCCAAGA TAACCTCAAA CTATGAAGTG 
ATTCGCAACC AACCCCTTAT GATAGCACCT GTTTCTACGG ATAACACATT TGCTGTTTCC 
CATCTTGGTA GTAAGTACAA TAGTTTAGAC TTGAGAATAG GTTTGGAGGA AAGAAGTAGC 
ATGGCAAGCA GTCCAATATC AAGCATATCT GCAGATTCTT TCTATGCAGA TATTGATCAC 
CATACTCCAC GAAATTATGT CCTAATTGAC GACATTGGAG AGATCACCAA AGGAACAGCG 
GCATTAAGCA CCGCATTTAG CCTTCATGAA AAGGATCTGT CAAAAACAGA CCGTCTCCTT 
CGAACCACTG AGACACGCCG GTCTCAAGAA GTGACAGATT TCCTAGCACC TTTACAGTCT 
TCCTCTAGAT TGCATAGTTA TGTGAAGGCG GAGGAAGACC CAATGGAGGA TCCTTACGAG 
TTAAAGCTTC TGAAACATCA GATTAAACAG GAATTTCGTA GAGGGACAGA GAGCTTAGAT 
CACCTTGCTG GTCTTTCTCA TTATTACCAT GCTGATACTA GCTACAGACA TTTTCCAAAA 
TCTGAGAAGT ATAGCATCAG TAGACTCACA CTTGAAAAAC AAGCAGCAAA ACAACTGCCA 
GCAGCCATAC TTTATCAAAA GCAGTCAAAG CATAAGAAAT CACTAATTGA CCCTAAAATG 
TCAAAATTTT CACCTATTCA AGAAAGTAGA GACCTTGAAC CTGATTATTC AAGCTATATG 
ACTTCTAGCA CTTCATCTAT TGGTGGCATT TCCTCCAGGG CAAGGCTCCT TCAAGATGAC 
ATCACTTTTG GCCTCAGAAA AAATATTACA GACCAACAAA AATTTATGGG ATCTTCTCTT 
GGCACAGGAC TGGGCACATT AGGAAATACC ATACGCTCAG CTCTGCAGGA TGAAGCGGAT 
AAGCCATACA GTAGTGGCAG CAGGTCCAGA CCTTCCTCCA GACCTTCCTC TGTCTATGGG 
CTTGATTTAT CAATTAAAAG GGATTCTTCT AGCTCTTCCC TAAGACTGAA AGCTCAAGAG 
GCTGAAGCTC TAGATGTTTC CTTTAGTCAT GCATCATCCT CTGCCAGAAC TAAGCCGACC 
AGTTTGCCAA TTAGTCAAAG TAGAGGAAGA ATACCAATTG TGGCCCAGAA TTCTGAAGAA 
GAAAGCCCAC TCAGTCCTGT TGGCCAGCCA ATGGGAATGG CCAGGGCTGC AGCTGGACCC 
CTGCCACCAA TATCTGCAGA CACCAGGGAT CAGTTTGGAT CAAGCCACTC ATTGCCTGAA 
GTTCAGCAAC ACATGAGGGA AGAATCACGG ACTCGAGGCT ATGACCGTGA CATAGCATTC 
ATCATGGATG ACTTCCAACA TGCCATGTCA GACAGTGAAG CCTATCATCT GCGTCGTGAG 
GAAACAGATT GGTTTGATAA ACCCAGGGAG TCTCGTTTGG AAAATGGACA TGGTCTGGAC 
CGAAAACTGC CGGAAAGATT GGTCCACTCT AGACCACTCA GTCAACATCA AGAGCAAATT 
ATACAGATGA ACGGGAAAAC TATGCACTAC ATCTTTCCTC ACGCAAGGAT AAAAATAACA 
AGAGACTCAA AGGATCACAC AGTTTCAGGT AATGGATTAG GAATTAGAAT TGTGGGTGGT 
AAAGAAATCC CGGGACATAG TGGAGAAATT GGAGCCTATA TTGCCAAGAT TCTTCCTGGG 
GGAAGTGCGG AACAGACGGG GAAGCTTATG GAAGGGATGC AAGTATTGGA ATGGAATGGA 
ATTCCCTTGA CTTCTAAAAC ATATGAAGAA GTTCAGAGTA TCATTAGTCA GCAAAGTGGG 
GAAGCAGAAA TATGTGTAAG ACTGGACCTC AATATGCTAT CAGATTCTGA AAATTCCCAG 
CATCTGGAAC TTCATGAGCC ACCAAAAGCT GTGGATAAGG CGAAATCCCC AGGGGTTGAT 
CCTAAGCAGT TGGCAGCAGA ACTCCAGAAG GTTTCACTAC AGCAGTCACC GCTGGTTCTG 
TCATCAGTTG TTGAAAAAGG ATCTCATGTT CATTCAGGTC CTACATCAGC AGGATCCAGT 
TCCGTTCCCA GCCCTGGGCA ACCAGGGTCC CCCTCAGTGA GCAAAAAGAA GCACGGCAGC 
AGCAAGCCTA CCGATGGAAC AAAGGTTGTC TCTCATCCAA TTACAGGAGA AATTCAGCTT 
CAAATTAACT ATGATCTTGG AAATCTCATA ATACATATTC TCCAAGCAAG AAATCTTGTT 
CCTCGAGACA ACAATGGTTA TTCTGACCCT TTTGTGAAAG TGTACCTTCT TCCAGGGAGA 
GGTCAAGTCA TGGTTGTCCA GAATGCAAGT GCTGAGTACA AGAGAAGGAC TAAACATGTC 
CAGAAAAGTC TTAATCCTGA GTGGAATCAA ACAGTAATTT ATAAAAGTAT TTCCATGGAA 
CAGCTCAAGA AGAAAACACT GGAGGTGACA GTTTGGGATT ATGATAGATT TTCATCCAAC 
GACTTCCTTG GGGAGGTATT GATTGATTTA TCTAGCACAT CTCACCTCGA TAACACTCCA 
AGGTGGTATC CTCTCAAAGA ACAGACTGAA AGCATTGATC ATGGCAAGTC TCATTCCAGT 
CAGAGCAGCC AGCAGTCCCC AAAGCCATCT GTTATCAAAA GCAGAAGCCA TGGTATCTTC 
CCTGACCCAT CAAAGGACAT GCAGGTTCCC ACCATTGAGA AATCCCATAG TAGTCCTGGT 
AGCTCAAAAT CATCATCAGA AGGCCATCTC CGTTCTCATG GACCATCTCG CAGTCAAAGC 
AAAACCAGCG TCACTCAGAC CCACCTGGAA GATGCAGGGG CTGCCATAGC TGCTGCCGAA 
GCTGCCGTGC AACAACTCCG CATTCAACCA AGTAAAAGAC GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055325.2
CDS	293..15100
Translation	MGNEASLEGEGLPEGLAAAAAAGGGASGAGSPSHTAIPAGMEADLSQLSEEERRQIAAVMSRAQGLPKGSVPPAAAESPSMHRKQELDSSHPPKQSGRPPDPGRPAQPGLSKSRTTDTFRSEQKLPGRSPSTISLKESKSRTDLKEEHKSSMMPGFLSEVNALSAVSSVVNKFNPFDLISDSEASQEETTKKQKVVQKEQGKPEGIIKPPLQQQPPKPIPKQQGPGRDPLQQDGTPKSISSQQPEKIKSQPPGTGKPIQGPTQTPQTDHAKLPLQRDASRPQTKQADIVRGESVKPSLPSPSKPPIQQPTPGKPPAQQPGHEKSQPGPAKPPAQPSGLTKPLAQQPGTVKPPVQPPGTTKPPAQPLGPAKPPAQQTGSEKPSSEQPGPKALAQPPGVGKTPAQQPGPAKPPTQQVGTPKPLAQQPGLQSPAKAPGPTKTPVQQPGPGKIPAQQAGPGKTSAQQTGPTKPPSQLPGPAKPPPQQPGPAKPPPQQPGSAKPPSQQPGSTKPPPQQPGPAKPSPQQPGSTKPPSQQPGSAKPSAQQPSPAKPSAQQSTKPVSQTGSGKPLQPPTVSPSAKQPPSQGLPKTICPLCNTTELLLHVPEKANFNTCTECQTTVCSLCGFNPNPHLTEVKEWLCLNCQMKRALGGDLAPVPSSPQPKLKTAPVTTTSAVSKSSPQPQQTSPKKDAAPKQDLSKAPEPKKPPPLVKQPTLHGSPSAKAKQPPEADSLSKPAPPKEPSVPSEQDKAPVADDKPKQPKMVKPTTDLVSSSSATTKPDIPSSKVQSQAEEKTTPPLKTDSAKPSQSFPPTGEKVSPFDSKAIPRPASDSKIISHPGPSSESKGQKQVDPVQKKEEPKKAQTKMSPKPDAKPMPKGSPTPPGPRPTAGQTVPTPQQSPKPQEQSRRFSLNLGSITDAPKSQPTTPQETVTGKLFGFGASIFSQASNLISTAGQPGPHSQSGPGAPMKQAPAPSQPPTSQGPPKSTGQAPPAPAKSIPVKKETKAPAAEKLEPKAEQAPTVKRTETEKKPPPIKDSKSLTAEPQKAVLPTKLEKSPKPESTCPLCKTELNIGSKDPPNFNTCTECKNQVCNLCGFNPTPHLTEIQEWLCLNCQTQRAISGQLGDIRKMPPAPSGPKASPMPVPTESSSQKTAVPPQVKLVKKQEQEVKTEAEKVILEKVKETLSMEKIPPMVTTDQKQEESKLEKDKASALQEKKPLPEEKKLIPEEEKIRSEEKKPLLEEKKPTPEDKKLLPEAKTSAPEEQKHDLLKSQVQIAEEKLEGRVAPKTVQEGKQPQTKMEGLPSGTPQSLPKEDDKTTKTIKEQPQPPCTAKPDQVEPGKEKTEKEDDKSDTSSSQQPKSPQGLSDTGYSSDGISSSLGEIPSLIPTDEKDILKGLKKDSFSQESSPSSPSDLAKLESTVLSILEAQASTLADEKSEKKTQPHEVSPEQPKDQEKTQSLSETLEITISEEEIKESQEERKDTFKKDSQQDIPSSKDHKEKSEFVDDITTRREPYDSVEESSESENSPVPQRKRRTSVGSSSSDEYKQEDSQGSGEEEDFIRKQIIEMSADEDASGSEDDEFIRNQLKEISSSTESQKKEETKGKGKITAGKHRRLTRKSSTSIDEDAGRRHSWHDEDDEAFDESPELKYRETKSQESEELVVTGGGGLRRFKTIELNSTIADKYSAESSQKKTSLYFDEEPELEMESLTDSPEDRSRGEGSSSLHASSFTPGTSPTSVSSLDEDSDSSPSHKKGESKQQRKARHRPHGPLLPTIEDSSEEEELREEEELLKEQEKQREIEQQQRKSSSKKSKKDKDELRAQRRRERPKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSPSIESDPEGFEISPEKIIEVQKVYKLPTAVSLYSPTDEQSIMQKEGSQKALKSAEEMYEEMMHKTHKYKAFPAANERDEVFEKEPLYGGMLIEDYIYESLVEDTYNGSVDGSLLTRQEEENGFMQQKGREQKIRLSEQIYEDPMQKITDLQKEFYELESLHSVVPQEDIVSSSFIIPESHEIVDLGTMVTSTEEERKLLDADAAYEELMKRQQMQLTPGSSPTQAPIGEDMTESTMDFDRMPDASLTSSVLSGASLTDSTSSATLSIPDVKITQHFSTEEIEDEYVTDYTREIQEIIAHESLILTYSEPSESATSVPPSDTPSLTSSVSSVCTTDSSSPITTLDSITTVYTEPVDMITKFEDSEEISSSTYFPGSIIDYPEEISVSLDRTAPPDGRASADHIVISLSDMASSIIESVVPKPEGPVADTVSTDLLISEKDPVKKAKKETGNGIILEVLEAYRDKKELEAERTKSSLSETVFDHPPSSVIALPMKEQLSTTYFTSGETFGQEKPASQLPSGSPSVSSLPAKPRPFFRSSSLDISAQPPPPPPPPPPPPPPPPPPPPPPLPPPTSPKPTILPKKKLTVASPVTTATPLFDAVTTLETTAVLRSNGLPVTRICTTAPPPVPPKPSSIPSGLVFTHRPEPSKPPIAPKPVIPQLPTTTQKPTDIHPKPTGLSLTSSMTLNLVTSADYKLPSPTSPLSPHSNKSSPRFSKSLTETYVVITLPSEPGTPTDSSASQAITSWPLGSPSKDLVSVEPVFSVVPPVTAVEIPISSEQTFYISGALQTFSATPVTAPSSFQAAPTSVTQFLTTEVSKTEVSATRSTAPSVGLSSISITIPPEPLALDNIHLEKPQYKEDGKLQLVGDVIDLRTVPKVEVKTTDKCIDLSASTMDVKRQITANEVYGKQISAVQPSIINLSVTSSIVTPVSLATETVTFVTCTASASYTTGTESLVGAEHAMTTPLQLTTSKHAEPPYRIPSDQVFPIAREEAPINLSLGTPAHAVTLAITKPVTVPPVGVTNGWTDSTVSQGITDGEVVDLSTTKSHRTVVTMDESTSSVMTKIIEDEKPVDLTAGRRAVCCDVVYKLPFGRSCTAQQPATTLPEDRFGYRDDHYQYDRSGPYGYRGIGGMKPSMSDTNLAEAGHFFYKSKNAFDYSEGTDTAVDLTSGRVTTGEVMDYSSKTTGPYPETRQVISGAGISTPQYSTARMTPPPGPQYCVGSVLRSSNGVVYSSVATPTPSTFAITTQPGSIFSTTVRDLSGIHTADAVTSLPAMHHSQPMPRSYFITTGASETDIAVTGIDISASLQTITMESLTAETIDSVPTLTTASEVFPEVVGDESALLIVPEEDKQQQQLDLERELLELEKIKQQRFAEELEWERQEIQRFREQEKIMVQKKLEELQSMKQHLLFQQEEERQAQFMMRQETLAQQQLQLEQIQQLQQQLHQQLEEQKIRQIYQYNYDPSGTASPQTTTEQAILEGQYAALEGSQFWATEDATTTASAVVAIEIPQSQGWYTVQSDGVTQYIAPPGILSTVSEIPLTDVVVKEEKQPKKRSSGAKVRGQYDDMGENMTDDPRSFKKIVDSGVQTDDEDATDRSYVSRRRRTKKSVDTSVQTDDEDQDEWDMPTRSRRKARVGKYGDSMTEADKTKPLSKVSSIAVQTVAEISVQTEPVGTIRTPSIRARVDAKVEIIKHISAPEKTYKGGSLGCQTEADSDTQSPQYLSATSPPKDKKRPTPLEIGYSSHLRADSTVQLAPSPPKSPKVLYSPISPLSPGKALESAFVPYEKPLPDDISPQKVLHPDMAKVPPASPKTAKMMQRSMSDPKPLSPTADESSRAPFQYTEGYTTKGSQTMTSSGAQKKVKRTLPNPPPEEISTGTQSTFSTMGTVSRRRICRTNTMARAKILQDIDRELDLVERESAKLRKKQAELDEEEKEIDAKLRYLEMGINRRKEALLKEREKRERAYLQGVAEDRDYMSDSEVSSTRPTRIESQHGIERPRTAPQTEFSQFIPPQTQTESQLVPPTSPYTQYQYSSPALPTQAPTSYTQQSHFEQQTLYHQQVSPYQTQPTFQAVATMSFTPQVQPTPTPQPSYQLPSQMMVIQQKPRQTTLYLEPKITSNYEVIRNQPLMIAPVSTDNTFAVSHLGSKYNSLDLRIGLEERSSMASSPISSISADSFYADIDHHTPRNYVLIDDIGEITKGTAALSTAFSLHEKDLSKTDRLLRTTETRRSQEVTDFLAPLQSSSRLHSYVKAEEDPMEDPYELKLLKHQIKQEFRRGTESLDHLAGLSHYYHADTSYRHFPKSEKYSISRLTLEKQAAKQLPAAILYQKQSKHKKSLIDPKMSKFSPIQESRDLEPDYSSYMTSSTSSIGGISSRARLLQDDITFGLRKNITDQQKFMGSSLGTGLGTLGNTIRSALQDEADKPYSSGSRSRPSSRPSSVYGLDLSIKRDSSSSSLRLKAQEAEALDVSFSHASSSARTKPTSLPISQSRGRIPIVAQNSEEESPLSPVGQPMGMARAAAGPLPPISADTRDQFGSSHSLPEVQQHMREESRTRGYDRDIAFIMDDFQHAMSDSEAYHLRREETDWFDKPRESRLENGHGLDRKLPERLVHSRPLSQHQEQIIQMNGKTMHYIFPHARIKITRDSKDHTVSGNGLGIRIVGGKEIPGHSGEIGAYIAKILPGGSAEQTGKLMEGMQVLEWNGIPLTSKTYEEVQSIISQQSGEAEICVRLDLNMLSDSENSQHLELHEPPKAVDKAKSPGVDPKQLAAELQKVSLQQSPLVLSSVVEKGSHVHSGPTSAGSSSVPSPGQPGSPSVSKKKHGSSKPTDGTKVVSHPITGEIQLQINYDLGNLIIHILQARNLVPRDNNGYSDPFVKVYLLPGRGQVMVVQNASAEYKRRTKHVQKSLNPEWNQTVIYKSISMEQLKKKTLEVTVWDYDRFSSNDFLGEVLIDLSSTSHLDNTPRWYPLKEQTESIDHGKSHSSQSSQQSPKPSVIKSRSHGIFPDPSKDMQVPTIEKSHSSPGSSKSSSEGHLRSHGPSRSQSKTSVTQTHLEDAGAAIAAAEAAVQQLRIQPSKRRK

Target ORF information:

RefSeq Version	NM_014510.3
Organism	Homo sapiens(human)
Definition	Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014510.3

ORF Insert Sequence:

ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA 
GCGGCTGGAG GAGGAGCTAG CGGGGCGGGG AGCCCCTCTC ACACCGCGAT CCCGGCCGGC 
ATGGAGGCGG ATTTGAGCCA GCTGAGCGAA GAGGAGAGGA GACAGATCGC CGCTGTCATG 
TCAAGGGCGC AGGGGCTGCC CAAGGGAAGC GTCCCCCCGG CCGCTGCGGA GTCGCCTTCC 
ATGCACAGGA AACAAGAGTT GGATAGTAGT CATCCTCCAA AGCAATCAGG AAGACCCCCG 
GACCCTGGGC GTCCAGCTCA ACCTGGTCTC AGTAAAAGTA GAACTACAGA CACTTTCAGG 
TCAGAGCAGA AATTGCCTGG GAGGAGTCCT TCCACTATTA GCTTGAAAGA ATCAAAGTCC 
AGAACTGATT TAAAGGAAGA GCACAAGTCT AGTATGATGC CTGGCTTCCT CTCAGAGGTT 
AACGCTTTAA GTGCTGTTTC CTCTGTTGTA AATAAATTCA ACCCTTTTGA TTTGATATCA 
GACTCTGAGG CATCCCAGGA AGAAACCACC AAGAAACAAA AAGTGGTTCA GAAGGAGCAA 
GGAAAACCTG AAGGAATCAT AAAACCTCCT TTACAACAAC AGCCACCCAA GCCGATTCCT 
AAGCAGCAAG GACCTGGTAG GGATCCGCTT CAGCAGGATG GCACTCCCAA ATCAATATCT 
TCTCAACAAC CAGAAAAAAT TAAATCACAA CCTCCAGGTA CAGGAAAGCC AATTCAGGGT 
CCTACCCAGA CTCCTCAGAC AGACCATGCA AAATTGCCAC TTCAACGAGA TGCATCCAGG 
CCTCAGACTA AACAGGCAGA CATAGTAAGG GGAGAATCAG TTAAACCCTC ACTGCCAAGC 
CCATCCAAAC CACCTATTCA GCAACCAACT CCTGGAAAAC CTCCAGCACA GCAGCCTGGA 
CATGAAAAAT CACAGCCTGG GCCTGCAAAG CCCCCAGCTC AGCCCTCAGG GCTAACAAAG 
CCATTGGCTC AACAACCAGG GACAGTGAAA CCCCCAGTCC AGCCACCAGG GACAACAAAG 
CCTCCAGCTC AGCCTCTTGG TCCTGCTAAG CCTCCAGCTC AGCAGACTGG GTCAGAGAAG 
CCTTCATCGG AGCAGCCTGG GCCAAAGGCT TTAGCTCAGC CTCCTGGAGT TGGAAAGACT 
CCAGCTCAAC AGCCAGGGCC AGCAAAGCCT CCAACCCAGC AGGTGGGGAC ACCAAAACCC 
CTAGCTCAAC AACCTGGGCT ACAGTCTCCA GCTAAGGCAC CTGGGCCTAC AAAGACTCCA 
GTTCAGCAGC CTGGGCCAGG AAAGATTCCA GCTCAACAGG CAGGACCCGG AAAGACTTCT 
GCCCAGCAGA CTGGCCCAAC AAAGCCTCCT TCACAACTGC CTGGCCCAGC AAAGCCCCCA 
CCTCAACAGC CTGGCCCAGC AAAGCCCCCA CCTCAACAGC CTGGCTCAGC AAAGCCCCCA 
TCTCAACAGC CTGGCTCAAC AAAACCCCCA CCTCAACAGC CTGGCCCAGC AAAGCCCTCA 
CCTCAACAGC CTGGCTCAAC AAAACCCCCA TCTCAACAGC CTGGCTCAGC AAAACCCTCA 
GCTCAACAGC CTAGCCCAGC AAAGCCCTCG GCTCAGCAAT CTACAAAACC AGTAAGCCAA 
ACAGGATCTG GAAAACCTCT GCAGCCACCA ACAGTGTCTC CATCTGCAAA ACAGCCTCCT 
TCACAAGGCC TCCCTAAAAC CATCTGTCCT CTTTGCAATA CCACTGAACT TCTGTTGCAT 
GTTCCAGAAA AGGCCAATTT TAACACATGC ACTGAGTGTC AAACCACTGT CTGTAGTCTC 
TGTGGTTTTA ATCCCAATCC TCATTTAACG GAGGTAAAAG AGTGGCTCTG TTTGAACTGT 
CAAATGAAAA GAGCTCTAGG CGGGGATCTG GCTCCAGTTC CGTCATCACC CCAGCCCAAA 
CTGAAGACTG CACCTGTTAC CACTACATCA GCAGTGAGCA AATCATCCCC ACAGCCACAG 
CAGACTTCCC CAAAGAAGGA TGCTGCACCA AAACAGGATC TCTCCAAGGC ACCTGAGCCT 
AAAAAGCCAC CACCACTAGT GAAACAACCA ACCCTTCATG GCTCTCCTTC AGCCAAGGCC 
AAGCAGCCCC CTGAGGCAGA TTCTTTGTCC AAGCCAGCCC CTCCCAAAGA ACCTTCTGTC 
CCATCTGAGC AGGACAAGGC CCCTGTTGCT GATGATAAAC CAAAGCAGCC CAAGATGGTA 
AAGCCAACCA CTGACCTTGT ATCTTCATCA TCAGCAACAA CAAAACCTGA TATTCCAAGC 
TCCAAAGTAC AGTCACAAGC TGAAGAGAAA ACAACCCCTC CTCTAAAAAC AGACTCTGCC 
AAACCCTCAC AGAGTTTTCC ACCAACAGGG GAAAAAGTCA GCCCATTTGA TTCTAAAGCC 
ATACCTCGAC CTGCATCAGA TTCAAAAATT ATTTCACATC CTGGTCCCAG TTCAGAGAGC 
AAAGGTCAAA AACAAGTTGA CCCCGTACAA AAGAAGGAAG AACCCAAGAA AGCACAAACC 
AAAATGAGTC CTAAACCAGA TGCCAAGCCA ATGCCAAAAG GGTCACCAAC ACCCCCTGGC 
CCACGACCTA CCGCTGGCCA AACTGTCCCC ACACCTCAAC AGTCCCCAAA GCCTCAGGAG 
CAGTCAAGGC GTTTCAGTCT GAATCTGGGA AGTATTACTG ATGCCCCCAA ATCACAGCCT 
ACAACTCCTC AAGAGACCGT GACTGGGAAA CTCTTTGGGT TTGGAGCATC AATCTTCAGC 
CAGGCATCAA ATTTAATTTC CACTGCAGGC CAACCTGGAC CTCATTCACA AAGTGGACCA 
GGGGCCCCAA TGAAACAAGC CCCTGCCCCT TCACAGCCAC CTACTTCACA AGGGCCACCC 
AAATCCACAG GTCAAGCACC ACCAGCACCT GCAAAAAGTA TACCTGTGAA AAAGGAAACA 
AAAGCCCCAG CAGCTGAAAA ATTAGAGCCC AAAGCTGAAC AAGCTCCAAC AGTAAAAAGA 
ACAGAAACAG AAAAAAAGCC ACCACCTATT AAGGATAGCA AATCTTTAAC AGCTGAGCCT 
CAAAAGGCTG TCCTTCCCAC AAAACTGGAG AAATCGCCCA AACCAGAATC AACCTGTCCT 
CTCTGCAAAA CTGAACTCAA CATAGGTTCT AAGGATCCTC CTAACTTCAA TACTTGCACT 
GAATGCAAGA ATCAAGTGTG TAATCTCTGT GGATTTAACC CTACACCACA TTTGACTGAG 
ATTCAAGAAT GGCTTTGTTT AAATTGCCAA ACCCAGAGAG CAATATCAGG ACAGCTTGGA 
GACATACGCA AAATGCCACC TGCACCATCA GGACCCAAAG CATCTCCTAT GCCTGTTCCT 
ACAGAATCAT CATCTCAGAA AACAGCAGTG CCTCCCCAAG TAAAATTAGT GAAAAAGCAA 
GAACAAGAAG TAAAAACGGA AGCTGAAAAA GTCATTCTGG AAAAAGTAAA GGAAACACTA 
TCAATGGAAA AAATTCCTCC TATGGTAACC ACAGATCAAA AACAAGAAGA GAGTAAACTA 
GAGAAAGACA AAGCTTCAGC TCTTCAAGAA AAAAAGCCAC TCCCTGAAGA AAAAAAACTA 
ATCCCTGAAG AAGAAAAGAT ACGTTCTGAA GAAAAAAAGC CACTCCTAGA AGAAAAAAAG 
CCAACCCCTG AAGACAAAAA GCTACTCCCA GAGGCAAAAA CATCAGCCCC AGAAGAACAG 
AAACATGACT TACTTAAATC TCAAGTACAA ATTGCTGAAG AAAAGCTTGA AGGCAGAGTG 
GCTCCAAAGA CAGTGCAAGA AGGGAAACAA CCACAGACCA AGATGGAAGG TTTACCATCT 
GGCACACCTC AGAGTTTACC TAAAGAAGAT GATAAGACAA CCAAAACAAT AAAAGAACAG 
CCACAGCCAC CATGCACAGC AAAACCTGAT CAGGTGGAAC CTGGGAAAGA AAAAACAGAA 
AAGGAAGATG ACAAATCAGA CACCTCAAGT TCTCAGCAGC CTAAAAGCCC CCAAGGTCTG 
AGCGACACGG GATATTCTTC CGATGGAATA TCAAGCTCAC TTGGTGAAAT TCCAAGTCTT 
ATTCCAACTG ATGAAAAGGA TATTCTCAAG GGACTCAAAA AGGACTCTTT TTCACAAGAA 
AGCAGCCCTT CCAGCCCCTC AGATTTGGCT AAGTTAGAAA GTACAGTCCT ATCTATTTTG 
GAAGCTCAAG CAAGTACACT TGCTGATGAA AAGTCAGAAA AGAAAACACA ACCCCATGAA 
GTTTCTCCTG AACAGCCTAA AGACCAAGAG AAAACTCAGA GTTTATCTGA AACCTTGGAA 
ATTACTATTT CAGAAGAGGA GATCAAAGAG AGTCAAGAAG AAAGGAAAGA CACTTTTAAA 
AAAGATAGCC AACAAGATAT TCCTTCCAGC AAGGACCATA AAGAGAAGTC TGAGTTTGTT 
GATGACATAA CTACTAGAAG AGAGCCTTAT GATTCAGTTG AAGAGAGTAG TGAAAGTGAA 
AACTCACCTG TTCCACAAAG AAAACGAAGA ACTAGTGTTG GCTCATCAAG CAGTGATGAG 
TATAAACAGG AAGACAGCCA AGGATCAGGG GAAGAGGAGG ACTTCATTCG AAAACAAATC 
ATAGAAATGA GTGCTGATGA AGATGCTTCA GGTTCTGAAG ATGATGAGTT CATCAGAAAC 
CAGCTCAAAG AGATTAGTAG CAGTACTGAG AGCCAGAAGA AGGAAGAAAC AAAGGGAAAA 
GGCAAAATAA CAGCAGGGAA ACACAGACGA CTGACTCGAA AAAGTAGCAC AAGCATTGAT 
GAAGATGCAG GAAGACGTCA CTCATGGCAT GATGAAGACG ATGAAGCATT TGATGAAAGT 
CCTGAACTTA AATACAGAGA AACTAAAAGT CAGGAAAGTG AAGAACTTGT AGTTACTGGA 
GGAGGAGGGC TACGCCGATT TAAAACAATT GAGCTCAACA GTACAATAGC AGATAAATAT 
TCTGCAGAGT CATCACAGAA AAAAACAAGT TTGTATTTTG ACGAAGAGCC AGAATTGGAA 
ATGGAAAGCC TGACAGACTC ACCTGAAGAT AGGTCAAGGG GAGAGGGATC TTCGAGTCTG 
CATGCTTCCA GCTTCACTCC TGGTACATCC CCTACATCAG TATCATCACT TGATGAGGAC 
AGTGACAGTA GCCCGAGTCA CAAAAAAGGA GAGAGCAAAC AGCAACGCAA AGCTCGGCAC 
AGACCACATG GCCCTCTTTT GCCTACTATT GAAGATTCTT CAGAGGAAGA AGAATTGAGA 
GAGGAAGAAG AATTATTAAA GGAGCAAGAA AAGCAGAGGG AAATAGAACA GCAACAAAGA 
AAGAGTTCTA GTAAAAAATC AAAGAAAGAC AAAGATGAAC TTCGAGCTCA GAGAAGAAGG 
GAAAGGCCAA AGACACCACC TAGTAATCTC TCTCCCATTG AAGATGCATC TCCGACAGAA 
GAGTTACGTC AGGCTGCAGA AATGGAGGAG CTCCATAGAT CTTCTTGTTC TGAATATTCA 
CCTAGCATAG AGTCAGACCC AGAAGGTTTT GAAATAAGCC CGGAAAAAAT AATAGAAGTA 
CAAAAAGTTT ATAAATTGCC CACAGCTGTT TCATTATACT CACCAACAGA TGAGCAATCT 
ATTATGCAGA AAGAAGGTAG CCAAAAGGCG TTAAAAAGTG CTGAGGAGAT GTATGAAGAA 
ATGATGCATA AAACACACAA ATACAAAGCT TTTCCAGCTG CAAATGAACG AGATGAAGTG 
TTTGAAAAAG AGCCTTTGTA TGGTGGGATG CTAATAGAGG ATTATATTTA TGAATCTTTA 
GTAGAAGACA CGTACAATGG ATCGGTAGAT GGCAGTCTGC TAACAAGGCA AGAAGAAGAA 
AATGGATTTA TGCAGCAGAA AGGAAGAGAG CAAAAGATAA GACTTTCAGA ACAGATTTAT 
GAAGATCCTA TGCAGAAAAT TACAGACCTC CAGAAAGAGT TTTATGAGTT AGAAAGCTTA 
CATTCTGTTG TGCCTCAGGA AGATATTGTT TCAAGCTCTT TTATCATCCC AGAAAGCCAT 
GAGATAGTGG ACCTGGGTAC TATGGTAACT TCTACAGAAG AAGAAAGGAA ACTACTAGAT 
GCTGATGCTG CCTATGAAGA ACTTATGAAG AGGCAACAGA TGCAATTAAC ACCTGGATCT 
AGCCCAACCC AGGCCCCCAT TGGTGAGGAT ATGACAGAGT CCACCATGGA CTTTGACAGA 
ATGCCAGATG CCTCTTTGAC ATCAAGTGTT CTCTCAGGAG CGTCTCTTAC AGATTCGACC 
AGCAGTGCAA CACTCTCTAT CCCAGATGTT AAAATAACCC AACATTTTTC AACAGAAGAA 
ATTGAGGATG AATATGTAAC CGATTATACA AGAGAAATTC AAGAGATAAT TGCCCATGAA 
TCGCTGATTT TGACCTACTC GGAGCCTTCA GAAAGTGCTA CATCTGTCCC ACCCTCTGAC 
ACACCTTCTC TCACATCATC TGTTTCTTCG GTCTGTACCA CAGATAGCTC TTCACCCATT 
ACTACCCTGG ATAGCATAAC CACAGTTTAT ACAGAGCCAG TGGACATGAT AACTAAATTT 
GAAGATTCTG AGGAAATTTC TTCATCAACT TATTTTCCAG GCAGCATTAT AGACTATCCA 
GAAGAAATAA GTGTATCTTT AGATCGGACT GCCCCACCAG ATGGTAGAGC TAGTGCTGAT 
CATATTGTTA TTTCCTTATC TGATATGGCA TCTTCTATCA TAGAATCTGT AGTACCTAAA 
CCTGAAGGGC CAGTTGCTGA CACTGTTTCT ACTGACTTAC TTATATCTGA AAAGGACCCA 
GTGAAGAAAG CCAAGAAGGA AACTGGGAAT GGAATCATTC TGGAAGTTTT GGAAGCTTAC 
AGAGATAAAA AGGAGTTGGA GGCCGAACGA ACAAAAAGTA GCTTATCCGA AACCGTGTTT 
GATCACCCAC CTTCTTCTGT AATAGCCCTT CCAATGAAAG AGCAGCTTTC AACTACATAC 
TTTACATCTG GAGAGACCTT TGGTCAGGAA AAACCTGCAT CTCAGTTACC ATCTGGCAGT 
CCTTCTGTTT CCTCTCTTCC AGCTAAACCT CGCCCATTCT TTAGAAGTTC TTCTTTGGAT 
ATATCAGCTC AACCTCCTCC CCCTCCTCCC CCTCCCCCTC CTCCTCCTCC TCCACCACCA 
CCCCCTCCTC CCCCACCACT TCCTCCACCA ACTTCACCTA AACCAACTAT TCTTCCTAAA 
AAAAAGTTAA CAGTTGCATC TCCAGTGACT ACAGCTACAC CTCTGTTTGA TGCTGTTACT 
ACTCTAGAGA CCACAGCTGT TCTGAGAAGT AATGGATTAC CTGTTACAAG AATATGTACT 
ACTGCACCTC CTCCTGTTCC TCCTAAGCCA TCTTCAATTC CATCTGGACT TGTATTTACC 
CACAGGCCTG AGCCAAGCAA ACCTCCAATC GCCCCCAAAC CAGTGATTCC TCAGCTTCCA 
ACAACTACAC AAAAACCAAC AGATATACAC CCCAAACCAA CAGGCCTATC TTTAACTTCA 
AGTATGACCT TAAATTTAGT GACTTCAGCA GATTATAAAT TGCCTTCCCC TACCTCCCCA 
CTTTCCCCAC ACTCCAACAA GTCTTCACCA AGATTTTCCA AATCCCTCAC AGAAACTTAT 
GTAGTTATTA CATTGCCATC TGAACCAGGG ACTCCAACAG ATTCTTCTGC TAGTCAAGCA 
ATTACCAGTT GGCCCTTGGG ATCACCCTCC AAAGATCTGG TTTCTGTTGA ACCTGTGTTT 
TCTGTAGTTC CTCCTGTGAC AGCTGTAGAA ATTCCAATTT CTTCAGAACA GACCTTCTAC 
ATCTCTGGAG CTTTACAGAC ATTTTCTGCT ACCCCTGTCA CAGCACCCTC TTCATTTCAA 
GCAGCTCCCA CATCAGTTAC ACAGTTTCTC ACTACTGAAG TTTCCAAGAC TGAGGTTTCA 
GCAACCAGAA GTACAGCTCC TAGTGTTGGT CTCAGCAGCA TTTCCATAAC AATTCCTCCA 
GAGCCTCTTG CTCTAGATAA CATACATTTA GAGAAGCCTC AGTATAAAGA AGATGGAAAA 
TTGCAACTTG TTGGTGATGT AATTGATTTG CGTACAGTAC CAAAGGTAGA AGTTAAAACA 
ACTGATAAAT GTATTGATCT TTCTGCTTCT ACAATGGATG TGAAAAGGCA GATCACAGCA 
AATGAAGTTT ATGGGAAACA AATTAGTGCT GTCCAACCCT CTATTATAAA TCTTAGTGTG 
ACATCATCAA TAGTGACTCC TGTATCTCTG GCCACTGAGA CAGTGACCTT TGTCACATGC 
ACAGCTAGTG CAAGTTACAC TACAGGCACA GAAAGCCTAG TGGGTGCAGA ACATGCAATG 
ACAACACCAC TCCAACTTAC AACATCAAAG CATGCTGAGC CCCCATACAG GATACCAAGT 
GACCAGGTCT TTCCTATAGC TAGGGAAGAA GCACCAATAA ACTTATCTCT AGGTACTCCA 
GCACATGCAG TGACATTGGC TATTACAAAA CCTGTCACTG TGCCTCCTGT TGGTGTCACA 
AATGGATGGA CTGATAGCAC CGTATCCCAG GGAATCACTG ATGGGGAAGT AGTGGATCTC 
AGTACAACCA AGTCTCACAG AACAGTCGTA ACAATGGATG AGTCTACTTC AAGTGTGATG 
ACCAAAATAA TAGAAGATGA AAAACCCGTT GATTTAACCG CAGGGAGAAG AGCTGTGTGC 
TGTGATGTGG TTTATAAATT ACCATTTGGA AGGAGCTGCA CAGCACAGCA GCCTGCAACT 
ACTCTTCCTG AGGATCGTTT TGGTTATAGG GATGACCACT ATCAGTATGA TCGATCAGGG 
CCATATGGTT ATAGAGGGAT TGGGGGAATG AAGCCTTCCA TGTCTGACAC AAATTTAGCA 
GAAGCTGGAC ATTTTTTCTA TAAAAGTAAG AATGCTTTTG ATTATTCTGA AGGAACTGAC 
ACAGCAGTAG ATCTGACTTC AGGGAGAGTT ACTACAGGTG AGGTAATGGA TTATTCAAGC 
AAGACTACAG GTCCATATCC AGAAACACGA CAAGTCATTT CAGGAGCTGG GATTAGTACC 
CCACAGTATT CCACAGCAAG AATGACACCA CCACCAGGAC CCCAGTATTG TGTGGGGAGT 
GTTTTGAGGT CATCTAATGG TGTTGTCTAT TCTTCAGTAG CAACTCCAAC ACCCTCTACA 
TTTGCTATCA CCACACAACC TGGCTCCATT TTCAGCACCA CAGTGAGGGA TTTGTCTGGT 
ATTCATACGG CTGATGCAGT GACTTCATTA CCTGCCATGC ACCATAGCCA GCCAATGCCT 
AGATCATATT TTATAACAAC AGGTGCATCT GAAACGGACA TTGCAGTAAC TGGTATTGAT 
ATCAGTGCCA GTTTGCAAAC TATTACTATG GAGTCTCTTA CTGCTGAGAC GATAGACTCT 
GTTCCCACTT TAACCACAGC ATCCGAAGTG TTTCCTGAAG TGGTGGGAGA TGAAAGTGCT 
CTTTTAATTG TCCCTGAAGA AGATAAACAA CAGCAGCAGC TAGACTTGGA GCGTGAGCTC 
CTGGAACTGG AGAAAATTAA GCAACAGCGC TTTGCTGAGG AATTGGAGTG GGAACGTCAG 
GAAATTCAAA GGTTCCGAGA ACAAGAAAAG ATCATGGTTC AGAAAAAGTT GGAGGAGCTG 
CAGTCTATGA AGCAACACCT TCTCTTTCAG CAAGAAGAAG AGCGGCAAGC CCAGTTCATG 
ATGAGGCAGG AGACGTTAGC TCAGCAACAG TTACAGCTTG AGCAGATCCA ACAGCTGCAA 
CAACAGCTTC ACCAGCAGCT GGAGGAGCAA AAGATTCGGC AGATCTACCA GTATAACTAT 
GACCCTTCTG GAACTGCTTC TCCACAAACC ACTACAGAGC AGGCAATTTT GGAAGGTCAG 
TATGCTGCTC TGGAAGGCAG TCAATTTTGG GCAACTGAAG ATGCAACCAC CACAGCTTCA 
GCTGTTGTGG CAATTGAAAT ACCACAAAGC CAAGGATGGT ACACCGTTCA GTCTGATGGT 
GTTACTCAGT ACATTGCCCC ACCTGGTATC CTGAGCACTG TTTCAGAAAT ACCTCTAACA 
GATGTTGTTG TGAAAGAGGA AAAACAACCC AAAAAGAGAA GTTCTGGAGC TAAAGTCCGA 
GGACAGTATG ATGACATGGG AGAAAATATG ACAGATGATC CCCGAAGTTT TAAAAAGATA 
GTGGACAGTG GTGTACAAAC GGATGACGAA GATGCCACAG ATCGGAGCTA TGTGAGTAGG 
AGAAGGAGAA CTAAAAAGAG TGTGGATACA AGCGTCCAAA CTGATGATGA AGATCAGGAT 
GAGTGGGATA TGCCTACTAG ATCAAGGAGG AAAGCTCGTG TAGGGAAATA TGGTGACAGC 
ATGACAGAGG CTGACAAGAC CAAACCCCTT TCCAAAGTCT CCAGCATAGC AGTTCAAACG 
GTAGCAGAGA TATCTGTGCA AACTGAACCA GTTGGAACCA TAAGAACACC CTCCATACGG 
GCACGAGTGG ATGCCAAGGT AGAAATAATT AAACACATTT CAGCACCTGA AAAGACTTAC 
AAAGGGGGCA GTTTAGGATG TCAAACAGAA GCAGATTCAG ACACACAAAG TCCTCAATAT 
CTGAGTGCCA CATCTCCACC CAAAGACAAG AAACGCCCAA CACCTTTAGA GATTGGTTAT 
TCATCTCACC TCCGGGCAGA TTCCACAGTA CAGCTGGCTC CTTCCCCACC CAAATCCCCC 
AAAGTCCTTT ACTCACCCAT CTCACCACTT TCACCAGGCA AAGCCTTAGA ATCAGCCTTT 
GTACCTTATG AAAAACCCCT CCCTGATGAT ATAAGTCCAC AGAAAGTACT GCATCCAGAT 
ATGGCTAAAG TTCCCCCAGC AAGTCCTAAG ACAGCCAAGA TGATGCAGCG TTCTATGTCT 
GACCCCAAGC CTCTGAGTCC AACAGCAGAC GAAAGTTCCA GGGCTCCTTT TCAGTATACC 
GAGGGCTATA CGACTAAAGG TTCTCAAACC ATGACATCCT CTGGAGCCCA GAAAAAAGTT 
AAAAGAACTC TGCCAAATCC ACCTCCTGAG GAGATTTCCA CAGGAACTCA ATCCACATTC 
AGCACAATGG GCACAGTTTC CAGGAGAAGG ATCTGCAGAA CCAACACAAT GGCACGAGCC 
AAGATTCTCC AGGACATAGA CAGAGAGCTT GATCTTGTGG AAAGGGAGTC TGCAAAACTT 
CGAAAGAAAC AAGCAGAGCT TGATGAAGAA GAAAAGGAGA TTGATGCTAA GCTACGATAC 
CTGGAAATGG GAATTAACAG GAGGAAAGAG GCCCTATTAA AGGAGAGAGA AAAGAGAGAA 
CGAGCCTACC TCCAGGGAGT AGCTGAGGAT CGTGATTACA TGTCTGACAG TGAAGTGAGT 
AGCACAAGAC CAACCCGAAT AGAAAGTCAG CATGGCATTG AGCGACCAAG AACTGCTCCC 
CAAACTGAAT TCAGCCAGTT TATACCACCA CAAACCCAAA CAGAATCTCA ACTAGTTCCT 
CCGACAAGTC CTTACACACA ATACCAGTAC TCTTCCCCTG CTCTTCCTAC CCAAGCACCC 
ACCTCATACA CTCAACAGTC TCATTTTGAG CAACAAACTT TGTACCATCA GCAAGTTTCA 
CCTTATCAGA CTCAGCCAAC ATTCCAAGCT GTGGCAACAA TGTCCTTCAC ACCTCAAGTT 
CAACCTACAC CAACCCCACA GCCTTCTTAT CAGTTACCTT CACAGATGAT GGTGATACAA 
CAGAAGCCAC GGCAAACTAC ATTATATTTG GAGCCCAAGA TAACCTCAAA CTATGAAGTG 
ATTCGCAACC AACCCCTTAT GATAGCACCT GTTTCTACGG ATAACACATT TGCTGTTTCC 
CATCTTGGTA GTAAGTACAA TAGTTTAGAC TTGAGAATAG GTTTGGAGGA AAGAAGTAGC 
ATGGCAAGCA GTCCAATATC AAGCATATCT GCAGATTCTT TCTATGCAGA TATTGATCAC 
CATACTCCAC GAAATTATGT CCTAATTGAC GACATTGGAG AGATCACCAA AGGAACAGCG 
GCATTAAGCA CCGCATTTAG CCTTCATGAA AAGGATCTGT CAAAAACAGA CCGTCTCCTT 
CGAACCACTG AGACACGCCG GTCTCAAGAA GTGACAGATT TCCTAGCACC TTTACAGTCT 
TCCTCTAGAT TGCATAGTTA TGTGAAGGCG GAGGAAGACC CAATGGAGGA TCCTTACGAG 
TTAAAGCTTC TGAAACATCA GATTAAACAG GAATTTCGTA GAGGGACAGA GAGCTTAGAT 
CACCTTGCTG GTCTTTCTCA TTATTACCAT GCTGATACTA GCTACAGACA TTTTCCAAAA 
TCTGAGAAGT ATAGCATCAG TAGACTCACA CTTGAAAAAC AAGCAGCAAA ACAACTGCCA 
GCAGCCATAC TTTATCAAAA GCAGTCAAAG CATAAGAAAT CACTAATTGA CCCTAAAATG 
TCAAAATTTT CACCTATTCA AGAAAGTAGA GACCTTGAAC CTGATTATTC AAGCTATATG 
ACTTCTAGCA CTTCATCTAT TGGTGGCATT TCCTCCAGGG CAAGGCTCCT TCAAGATGAC 
ATCACTTTTG GCCTCAGAAA AAATATTACA GACCAACAAA AATTTATGGG ATCTTCTCTT 
GGCACAGGAC TGGGCACATT AGGAAATACC ATACGCTCAG CTCTGCAGGA TGAAGCGGAT 
AAGCCATACA GTAGTGGCAG CAGGTCCAGA CCTTCCTCCA GACCTTCCTC TGTCTATGGG 
CTTGATTTAT CAATTAAAAG GGATTCTTCT AGCTCTTCCC TAAGACTGAA AGCTCAAGAG 
GCTGAAGCTC TAGATGTTTC CTTTAGTCAT GCATCATCCT CTGCCAGAAC TAAGCCGACC 
AGTTTGCCAA TTAGTCAAAG TAGAGGAAGA ATACCAATTG TGGCCCAGAA TTCTGAAGAA 
GAAAGCCCAC TCAGTCCTGT TGGCCAGCCA ATGGGAATGG CCAGGGCTGC AGCTGGACCC 
CTGCCACCAA TATCTGCAGA CACCAGGGAT CAGTTTGGAT CAAGCCACTC ATTGCCTGAA 
GTTCAGCAAC ACATGAGGGA AGAATCACGG ACTCGAGGCT ATGACCGTGA CATAGCATTC 
ATCATGGATG ACTTCCAACA TGCCATGTCA GACAGTGAAG CCTATCATCT GCGTCGTGAG 
GAAACAGATT GGTTTGATAA ACCCAGGGAG TCTCGTTTGG AAAATGGACA TGGTCTGGAC 
CGAAAACTGC CGGAAAGATT GGTCCACTCT AGACCACTCA GTCAACATCA AGAGCAAATT 
ATACAGATGA ACGGGAAAAC TATGCACTAC ATCTTTCCTC ACGCAAGGAT AAAAATAACA 
AGAGACTCAA AGGATCACAC AGTTTCAGGT AATGGATTAG GAATTAGAAT TGTGGGTGGT 
AAAGAAATCC CGGGACATAG TGGAGAAATT GGAGCCTATA TTGCCAAGAT TCTTCCTGGG 
GGAAGTGCGG AACAGACGGG GAAGCTTATG GAAGGGATGC AAGTATTGGA ATGGAATGGA 
ATTCCCTTGA CTTCTAAAAC ATATGAAGAA GTTCAGAGTA TCATTAGTCA GCAAAGTGGG 
GAAGCAGAAA TATGTGTAAG ACTGGACCTC AATATGCTAT CAGATTCTGA AAATTCCCAG 
CATCTGGAAC TTCATGAGCC ACCAAAAGCT GTGGATAAGG CGAAATCCCC AGGGGTTGAT 
CCTAAGCAGT TGGCAGCAGA ACTCCAGAAG GTTTCACTAC AGCAGTCACC GCTGGTTCTG 
TCATCAGTTG TTGAAAAAGG ATCTCATGTT CATTCAGGTC CTACATCAGC AGGATCCAGT 
TCCGTTCCCA GCCCTGGGCA ACCAGGGTCC CCCTCAGTGA GCAAAAAGAA GCACGGCAGC 
AGCAAGCCTA CCGATGGAAC AAAGGTTGTC TCTCATCCAA TTACAGGAGA AATTCAGCTT 
CAAATTAACT ATGATCTTGG AAATCTCATA ATACATATTC TCCAAGCAAG AAATCTTGTT 
CCTCGAGACA ACAATGGTTA TTCTGACCCT TTTGTGAAAG TGTACCTTCT TCCAGGGAGA 
GGTCAAGTCA TGGTTGTCCA GAATGCAAGT GCTGAGTACA AGAGAAGGAC TAAACATGTC 
CAGAAAAGTC TTAATCCTGA GTGGAATCAA ACAGTAATTT ATAAAAGTAT TTCCATGGAA 
CAGCTCAAGA AGAAAACACT GGAGGTGACA GTTTGGGATT ATGATAGATT TTCATCCAAC 
GACTTCCTTG GGGAGGTATT GATTGATTTA TCTAGCACAT CTCACCTCGA TAACACTCCA 
AGGTGGTATC CTCTCAAAGA ACAGACTGAA AGCATTGATC ATGGCAAGTC TCATTCCAGT 
CAGAGCAGCC AGCAGTCCCC AAAGCCATCT GTTATCAAAA GCAGAAGCCA TGGTATCTTC 
CCTGACCCAT CAAAGGACAT GCAGGTTCCC ACCATTGAGA AATCCCATAG TAGTCCTGGT 
AGCTCAAAAT CATCATCAGA AGGCCATCTC CGTTCTCATG GACCATCTCG CAGTCAAAGC 
AAAACCAGCG TCACTCAGAC CCACCTGGAA GATGCAGGGG CTGCCATAGC TGCTGCCGAA 
GCTGCCGTGC AACAACTCCG CATTCAACCA AGTAAAAGAC GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCLO ( NM_014510.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055325.2 Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant 2, mRNA.

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CloneID	OHu24261
Clone ID Related Accession (Same CDS sequence)	NM_014510.3 , NM_014510.2
Accession Version	NM_014510.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	protein piccolo isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093461.4, AC004903.2, AC004886.1, DN990884.1, AB011131.1 and BG913494.1. On or before Jul 1, 2007 this sequence version replaced XM_929946.2, XM_940805.2, XM_935710.2, NM_014510.1. Transcript Variant: This variant (2) lacks several 3' terminal exons and differs at the 3' end compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA 
GCGGCTGGAG GAGGAGCTAG CGGGGCGGGG AGCCCCTCTC ACACCGCGAT CCCGGCCGGC 
ATGGAGGCGG ATTTGAGCCA GCTGAGCGAA GAGGAGAGGA GACAGATCGC CGCTGTCATG 
TCAAGGGCGC AGGGGCTGCC CAAGGGAAGC GTCCCCCCGG CCGCTGCGGA GTCGCCTTCC 
ATGCACAGGA AACAAGAGTT GGATAGTAGT CATCCTCCAA AGCAATCAGG AAGACCCCCG 
GACCCTGGGC GTCCAGCTCA ACCTGGTCTC AGTAAAAGTA GAACTACAGA CACTTTCAGG 
TCAGAGCAGA AATTGCCTGG GAGGAGTCCT TCCACTATTA GCTTGAAAGA ATCAAAGTCC 
AGAACTGATT TAAAGGAAGA GCACAAGTCT AGTATGATGC CTGGCTTCCT CTCAGAGGTT 
AACGCTTTAA GTGCTGTTTC CTCTGTTGTA AATAAATTCA ACCCTTTTGA TTTGATATCA 
GACTCTGAGG CATCCCAGGA AGAAACCACC AAGAAACAAA AAGTGGTTCA GAAGGAGCAA 
GGAAAACCTG AAGGAATCAT AAAACCTCCT TTACAACAAC AGCCACCCAA GCCGATTCCT 
AAGCAGCAAG GACCTGGTAG GGATCCGCTT CAGCAGGATG GCACTCCCAA ATCAATATCT 
TCTCAACAAC CAGAAAAAAT TAAATCACAA CCTCCAGGTA CAGGAAAGCC AATTCAGGGT 
CCTACCCAGA CTCCTCAGAC AGACCATGCA AAATTGCCAC TTCAACGAGA TGCATCCAGG 
CCTCAGACTA AACAGGCAGA CATAGTAAGG GGAGAATCAG TTAAACCCTC ACTGCCAAGC 
CCATCCAAAC CACCTATTCA GCAACCAACT CCTGGAAAAC CTCCAGCACA GCAGCCTGGA 
CATGAAAAAT CACAGCCTGG GCCTGCAAAG CCCCCAGCTC AGCCCTCAGG GCTAACAAAG 
CCATTGGCTC AACAACCAGG GACAGTGAAA CCCCCAGTCC AGCCACCAGG GACAACAAAG 
CCTCCAGCTC AGCCTCTTGG TCCTGCTAAG CCTCCAGCTC AGCAGACTGG GTCAGAGAAG 
CCTTCATCGG AGCAGCCTGG GCCAAAGGCT TTAGCTCAGC CTCCTGGAGT TGGAAAGACT 
CCAGCTCAAC AGCCAGGGCC AGCAAAGCCT CCAACCCAGC AGGTGGGGAC ACCAAAACCC 
CTAGCTCAAC AACCTGGGCT ACAGTCTCCA GCTAAGGCAC CTGGGCCTAC AAAGACTCCA 
GTTCAGCAGC CTGGGCCAGG AAAGATTCCA GCTCAACAGG CAGGACCCGG AAAGACTTCT 
GCCCAGCAGA CTGGCCCAAC AAAGCCTCCT TCACAACTGC CTGGCCCAGC AAAGCCCCCA 
CCTCAACAGC CTGGCCCAGC AAAGCCCCCA CCTCAACAGC CTGGCTCAGC AAAGCCCCCA 
TCTCAACAGC CTGGCTCAAC AAAACCCCCA CCTCAACAGC CTGGCCCAGC AAAGCCCTCA 
CCTCAACAGC CTGGCTCAAC AAAACCCCCA TCTCAACAGC CTGGCTCAGC AAAACCCTCA 
GCTCAACAGC CTAGCCCAGC AAAGCCCTCG GCTCAGCAAT CTACAAAACC AGTAAGCCAA 
ACAGGATCTG GAAAACCTCT GCAGCCACCA ACAGTGTCTC CATCTGCAAA ACAGCCTCCT 
TCACAAGGCC TCCCTAAAAC CATCTGTCCT CTTTGCAATA CCACTGAACT TCTGTTGCAT 
GTTCCAGAAA AGGCCAATTT TAACACATGC ACTGAGTGTC AAACCACTGT CTGTAGTCTC 
TGTGGTTTTA ATCCCAATCC TCATTTAACG GAGGTAAAAG AGTGGCTCTG TTTGAACTGT 
CAAATGAAAA GAGCTCTAGG CGGGGATCTG GCTCCAGTTC CGTCATCACC CCAGCCCAAA 
CTGAAGACTG CACCTGTTAC CACTACATCA GCAGTGAGCA AATCATCCCC ACAGCCACAG 
CAGACTTCCC CAAAGAAGGA TGCTGCACCA AAACAGGATC TCTCCAAGGC ACCTGAGCCT 
AAAAAGCCAC CACCACTAGT GAAACAACCA ACCCTTCATG GCTCTCCTTC AGCCAAGGCC 
AAGCAGCCCC CTGAGGCAGA TTCTTTGTCC AAGCCAGCCC CTCCCAAAGA ACCTTCTGTC 
CCATCTGAGC AGGACAAGGC CCCTGTTGCT GATGATAAAC CAAAGCAGCC CAAGATGGTA 
AAGCCAACCA CTGACCTTGT ATCTTCATCA TCAGCAACAA CAAAACCTGA TATTCCAAGC 
TCCAAAGTAC AGTCACAAGC TGAAGAGAAA ACAACCCCTC CTCTAAAAAC AGACTCTGCC 
AAACCCTCAC AGAGTTTTCC ACCAACAGGG GAAAAAGTCA GCCCATTTGA TTCTAAAGCC 
ATACCTCGAC CTGCATCAGA TTCAAAAATT ATTTCACATC CTGGTCCCAG TTCAGAGAGC 
AAAGGTCAAA AACAAGTTGA CCCCGTACAA AAGAAGGAAG AACCCAAGAA AGCACAAACC 
AAAATGAGTC CTAAACCAGA TGCCAAGCCA ATGCCAAAAG GGTCACCAAC ACCCCCTGGC 
CCACGACCTA CCGCTGGCCA AACTGTCCCC ACACCTCAAC AGTCCCCAAA GCCTCAGGAG 
CAGTCAAGGC GTTTCAGTCT GAATCTGGGA AGTATTACTG ATGCCCCCAA ATCACAGCCT 
ACAACTCCTC AAGAGACCGT GACTGGGAAA CTCTTTGGGT TTGGAGCATC AATCTTCAGC 
CAGGCATCAA ATTTAATTTC CACTGCAGGC CAACCTGGAC CTCATTCACA AAGTGGACCA 
GGGGCCCCAA TGAAACAAGC CCCTGCCCCT TCACAGCCAC CTACTTCACA AGGGCCACCC 
AAATCCACAG GTCAAGCACC ACCAGCACCT GCAAAAAGTA TACCTGTGAA AAAGGAAACA 
AAAGCCCCAG CAGCTGAAAA ATTAGAGCCC AAAGCTGAAC AAGCTCCAAC AGTAAAAAGA 
ACAGAAACAG AAAAAAAGCC ACCACCTATT AAGGATAGCA AATCTTTAAC AGCTGAGCCT 
CAAAAGGCTG TCCTTCCCAC AAAACTGGAG AAATCGCCCA AACCAGAATC AACCTGTCCT 
CTCTGCAAAA CTGAACTCAA CATAGGTTCT AAGGATCCTC CTAACTTCAA TACTTGCACT 
GAATGCAAGA ATCAAGTGTG TAATCTCTGT GGATTTAACC CTACACCACA TTTGACTGAG 
ATTCAAGAAT GGCTTTGTTT AAATTGCCAA ACCCAGAGAG CAATATCAGG ACAGCTTGGA 
GACATACGCA AAATGCCACC TGCACCATCA GGACCCAAAG CATCTCCTAT GCCTGTTCCT 
ACAGAATCAT CATCTCAGAA AACAGCAGTG CCTCCCCAAG TAAAATTAGT GAAAAAGCAA 
GAACAAGAAG TAAAAACGGA AGCTGAAAAA GTCATTCTGG AAAAAGTAAA GGAAACACTA 
TCAATGGAAA AAATTCCTCC TATGGTAACC ACAGATCAAA AACAAGAAGA GAGTAAACTA 
GAGAAAGACA AAGCTTCAGC TCTTCAAGAA AAAAAGCCAC TCCCTGAAGA AAAAAAACTA 
ATCCCTGAAG AAGAAAAGAT ACGTTCTGAA GAAAAAAAGC CACTCCTAGA AGAAAAAAAG 
CCAACCCCTG AAGACAAAAA GCTACTCCCA GAGGCAAAAA CATCAGCCCC AGAAGAACAG 
AAACATGACT TACTTAAATC TCAAGTACAA ATTGCTGAAG AAAAGCTTGA AGGCAGAGTG 
GCTCCAAAGA CAGTGCAAGA AGGGAAACAA CCACAGACCA AGATGGAAGG TTTACCATCT 
GGCACACCTC AGAGTTTACC TAAAGAAGAT GATAAGACAA CCAAAACAAT AAAAGAACAG 
CCACAGCCAC CATGCACAGC AAAACCTGAT CAGGTGGAAC CTGGGAAAGA AAAAACAGAA 
AAGGAAGATG ACAAATCAGA CACCTCAAGT TCTCAGCAGC CTAAAAGCCC CCAAGGTCTG 
AGCGACACGG GATATTCTTC CGATGGAATA TCAAGCTCAC TTGGTGAAAT TCCAAGTCTT 
ATTCCAACTG ATGAAAAGGA TATTCTCAAG GGACTCAAAA AGGACTCTTT TTCACAAGAA 
AGCAGCCCTT CCAGCCCCTC AGATTTGGCT AAGTTAGAAA GTACAGTCCT ATCTATTTTG 
GAAGCTCAAG CAAGTACACT TGCTGATGAA AAGTCAGAAA AGAAAACACA ACCCCATGAA 
GTTTCTCCTG AACAGCCTAA AGACCAAGAG AAAACTCAGA GTTTATCTGA AACCTTGGAA 
ATTACTATTT CAGAAGAGGA GATCAAAGAG AGTCAAGAAG AAAGGAAAGA CACTTTTAAA 
AAAGATAGCC AACAAGATAT TCCTTCCAGC AAGGACCATA AAGAGAAGTC TGAGTTTGTT 
GATGACATAA CTACTAGAAG AGAGCCTTAT GATTCAGTTG AAGAGAGTAG TGAAAGTGAA 
AACTCACCTG TTCCACAAAG AAAACGAAGA ACTAGTGTTG GCTCATCAAG CAGTGATGAG 
TATAAACAGG AAGACAGCCA AGGATCAGGG GAAGAGGAGG ACTTCATTCG AAAACAAATC 
ATAGAAATGA GTGCTGATGA AGATGCTTCA GGTTCTGAAG ATGATGAGTT CATCAGAAAC 
CAGCTCAAAG AGATTAGTAG CAGTACTGAG AGCCAGAAGA AGGAAGAAAC AAAGGGAAAA 
GGCAAAATAA CAGCAGGGAA ACACAGACGA CTGACTCGAA AAAGTAGCAC AAGCATTGAT 
GAAGATGCAG GAAGACGTCA CTCATGGCAT GATGAAGACG ATGAAGCATT TGATGAAAGT 
CCTGAACTTA AATACAGAGA AACTAAAAGT CAGGAAAGTG AAGAACTTGT AGTTACTGGA 
GGAGGAGGGC TACGCCGATT TAAAACAATT GAGCTCAACA GTACAATAGC AGATAAATAT 
TCTGCAGAGT CATCACAGAA AAAAACAAGT TTGTATTTTG ACGAAGAGCC AGAATTGGAA 
ATGGAAAGCC TGACAGACTC ACCTGAAGAT AGGTCAAGGG GAGAGGGATC TTCGAGTCTG 
CATGCTTCCA GCTTCACTCC TGGTACATCC CCTACATCAG TATCATCACT TGATGAGGAC 
AGTGACAGTA GCCCGAGTCA CAAAAAAGGA GAGAGCAAAC AGCAACGCAA AGCTCGGCAC 
AGACCACATG GCCCTCTTTT GCCTACTATT GAAGATTCTT CAGAGGAAGA AGAATTGAGA 
GAGGAAGAAG AATTATTAAA GGAGCAAGAA AAGCAGAGGG AAATAGAACA GCAACAAAGA 
AAGAGTTCTA GTAAAAAATC AAAGAAAGAC AAAGATGAAC TTCGAGCTCA GAGAAGAAGG 
GAAAGGCCAA AGACACCACC TAGTAATCTC TCTCCCATTG AAGATGCATC TCCGACAGAA 
GAGTTACGTC AGGCTGCAGA AATGGAGGAG CTCCATAGAT CTTCTTGTTC TGAATATTCA 
CCTAGCATAG AGTCAGACCC AGAAGGTTTT GAAATAAGCC CGGAAAAAAT AATAGAAGTA 
CAAAAAGTTT ATAAATTGCC CACAGCTGTT TCATTATACT CACCAACAGA TGAGCAATCT 
ATTATGCAGA AAGAAGGTAG CCAAAAGGCG TTAAAAAGTG CTGAGGAGAT GTATGAAGAA 
ATGATGCATA AAACACACAA ATACAAAGCT TTTCCAGCTG CAAATGAACG AGATGAAGTG 
TTTGAAAAAG AGCCTTTGTA TGGTGGGATG CTAATAGAGG ATTATATTTA TGAATCTTTA 
GTAGAAGACA CGTACAATGG ATCGGTAGAT GGCAGTCTGC TAACAAGGCA AGAAGAAGAA 
AATGGATTTA TGCAGCAGAA AGGAAGAGAG CAAAAGATAA GACTTTCAGA ACAGATTTAT 
GAAGATCCTA TGCAGAAAAT TACAGACCTC CAGAAAGAGT TTTATGAGTT AGAAAGCTTA 
CATTCTGTTG TGCCTCAGGA AGATATTGTT TCAAGCTCTT TTATCATCCC AGAAAGCCAT 
GAGATAGTGG ACCTGGGTAC TATGGTAACT TCTACAGAAG AAGAAAGGAA ACTACTAGAT 
GCTGATGCTG CCTATGAAGA ACTTATGAAG AGGCAACAGA TGCAATTAAC ACCTGGATCT 
AGCCCAACCC AGGCCCCCAT TGGTGAGGAT ATGACAGAGT CCACCATGGA CTTTGACAGA 
ATGCCAGATG CCTCTTTGAC ATCAAGTGTT CTCTCAGGAG CGTCTCTTAC AGATTCGACC 
AGCAGTGCAA CACTCTCTAT CCCAGATGTT AAAATAACCC AACATTTTTC AACAGAAGAA 
ATTGAGGATG AATATGTAAC CGATTATACA AGAGAAATTC AAGAGATAAT TGCCCATGAA 
TCGCTGATTT TGACCTACTC GGAGCCTTCA GAAAGTGCTA CATCTGTCCC ACCCTCTGAC 
ACACCTTCTC TCACATCATC TGTTTCTTCG GTCTGTACCA CAGATAGCTC TTCACCCATT 
ACTACCCTGG ATAGCATAAC CACAGTTTAT ACAGAGCCAG TGGACATGAT AACTAAATTT 
GAAGATTCTG AGGAAATTTC TTCATCAACT TATTTTCCAG GCAGCATTAT AGACTATCCA 
GAAGAAATAA GTGTATCTTT AGATCGGACT GCCCCACCAG ATGGTAGAGC TAGTGCTGAT 
CATATTGTTA TTTCCTTATC TGATATGGCA TCTTCTATCA TAGAATCTGT AGTACCTAAA 
CCTGAAGGGC CAGTTGCTGA CACTGTTTCT ACTGACTTAC TTATATCTGA AAAGGACCCA 
GTGAAGAAAG CCAAGAAGGA AACTGGGAAT GGAATCATTC TGGAAGTTTT GGAAGCTTAC 
AGAGATAAAA AGGAGTTGGA GGCCGAACGA ACAAAAAGTA GCTTATCCGA AACCGTGTTT 
GATCACCCAC CTTCTTCTGT AATAGCCCTT CCAATGAAAG AGCAGCTTTC AACTACATAC 
TTTACATCTG GAGAGACCTT TGGTCAGGAA AAACCTGCAT CTCAGTTACC ATCTGGCAGT 
CCTTCTGTTT CCTCTCTTCC AGCTAAACCT CGCCCATTCT TTAGAAGTTC TTCTTTGGAT 
ATATCAGCTC AACCTCCTCC CCCTCCTCCC CCTCCCCCTC CTCCTCCTCC TCCACCACCA 
CCCCCTCCTC CCCCACCACT TCCTCCACCA ACTTCACCTA AACCAACTAT TCTTCCTAAA 
AAAAAGTTAA CAGTTGCATC TCCAGTGACT ACAGCTACAC CTCTGTTTGA TGCTGTTACT 
ACTCTAGAGA CCACAGCTGT TCTGAGAAGT AATGGATTAC CTGTTACAAG AATATGTACT 
ACTGCACCTC CTCCTGTTCC TCCTAAGCCA TCTTCAATTC CATCTGGACT TGTATTTACC 
CACAGGCCTG AGCCAAGCAA ACCTCCAATC GCCCCCAAAC CAGTGATTCC TCAGCTTCCA 
ACAACTACAC AAAAACCAAC AGATATACAC CCCAAACCAA CAGGCCTATC TTTAACTTCA 
AGTATGACCT TAAATTTAGT GACTTCAGCA GATTATAAAT TGCCTTCCCC TACCTCCCCA 
CTTTCCCCAC ACTCCAACAA GTCTTCACCA AGATTTTCCA AATCCCTCAC AGAAACTTAT 
GTAGTTATTA CATTGCCATC TGAACCAGGG ACTCCAACAG ATTCTTCTGC TAGTCAAGCA 
ATTACCAGTT GGCCCTTGGG ATCACCCTCC AAAGATCTGG TTTCTGTTGA ACCTGTGTTT 
TCTGTAGTTC CTCCTGTGAC AGCTGTAGAA ATTCCAATTT CTTCAGAACA GACCTTCTAC 
ATCTCTGGAG CTTTACAGAC ATTTTCTGCT ACCCCTGTCA CAGCACCCTC TTCATTTCAA 
GCAGCTCCCA CATCAGTTAC ACAGTTTCTC ACTACTGAAG TTTCCAAGAC TGAGGTTTCA 
GCAACCAGAA GTACAGCTCC TAGTGTTGGT CTCAGCAGCA TTTCCATAAC AATTCCTCCA 
GAGCCTCTTG CTCTAGATAA CATACATTTA GAGAAGCCTC AGTATAAAGA AGATGGAAAA 
TTGCAACTTG TTGGTGATGT AATTGATTTG CGTACAGTAC CAAAGGTAGA AGTTAAAACA 
ACTGATAAAT GTATTGATCT TTCTGCTTCT ACAATGGATG TGAAAAGGCA GATCACAGCA 
AATGAAGTTT ATGGGAAACA AATTAGTGCT GTCCAACCCT CTATTATAAA TCTTAGTGTG 
ACATCATCAA TAGTGACTCC TGTATCTCTG GCCACTGAGA CAGTGACCTT TGTCACATGC 
ACAGCTAGTG CAAGTTACAC TACAGGCACA GAAAGCCTAG TGGGTGCAGA ACATGCAATG 
ACAACACCAC TCCAACTTAC AACATCAAAG CATGCTGAGC CCCCATACAG GATACCAAGT 
GACCAGGTCT TTCCTATAGC TAGGGAAGAA GCACCAATAA ACTTATCTCT AGGTACTCCA 
GCACATGCAG TGACATTGGC TATTACAAAA CCTGTCACTG TGCCTCCTGT TGGTGTCACA 
AATGGATGGA CTGATAGCAC CGTATCCCAG GGAATCACTG ATGGGGAAGT AGTGGATCTC 
AGTACAACCA AGTCTCACAG AACAGTCGTA ACAATGGATG AGTCTACTTC AAGTGTGATG 
ACCAAAATAA TAGAAGATGA AAAACCCGTT GATTTAACCG CAGGGAGAAG AGCTGTGTGC 
TGTGATGTGG TTTATAAATT ACCATTTGGA AGGAGCTGCA CAGCACAGCA GCCTGCAACT 
ACTCTTCCTG AGGATCGTTT TGGTTATAGG GATGACCACT ATCAGTATGA TCGATCAGGG 
CCATATGGTT ATAGAGGGAT TGGGGGAATG AAGCCTTCCA TGTCTGACAC AAATTTAGCA 
GAAGCTGGAC ATTTTTTCTA TAAAAGTAAG AATGCTTTTG ATTATTCTGA AGGAACTGAC 
ACAGCAGTAG ATCTGACTTC AGGGAGAGTT ACTACAGGTG AGGTAATGGA TTATTCAAGC 
AAGACTACAG GTCCATATCC AGAAACACGA CAAGTCATTT CAGGAGCTGG GATTAGTACC 
CCACAGTATT CCACAGCAAG AATGACACCA CCACCAGGAC CCCAGTATTG TGTGGGGAGT 
GTTTTGAGGT CATCTAATGG TGTTGTCTAT TCTTCAGTAG CAACTCCAAC ACCCTCTACA 
TTTGCTATCA CCACACAACC TGGCTCCATT TTCAGCACCA CAGTGAGGGA TTTGTCTGGT 
ATTCATACGG CTGATGCAGT GACTTCATTA CCTGCCATGC ACCATAGCCA GCCAATGCCT 
AGATCATATT TTATAACAAC AGGTGCATCT GAAACGGACA TTGCAGTAAC TGGTATTGAT 
ATCAGTGCCA GTTTGCAAAC TATTACTATG GAGTCTCTTA CTGCTGAGAC GATAGACTCT 
GTTCCCACTT TAACCACAGC ATCCGAAGTG TTTCCTGAAG TGGTGGGAGA TGAAAGTGCT 
CTTTTAATTG TCCCTGAAGA AGATAAACAA CAGCAGCAGC TAGACTTGGA GCGTGAGCTC 
CTGGAACTGG AGAAAATTAA GCAACAGCGC TTTGCTGAGG AATTGGAGTG GGAACGTCAG 
GAAATTCAAA GGTTCCGAGA ACAAGAAAAG ATCATGGTTC AGAAAAAGTT GGAGGAGCTG 
CAGTCTATGA AGCAACACCT TCTCTTTCAG CAAGAAGAAG AGCGGCAAGC CCAGTTCATG 
ATGAGGCAGG AGACGTTAGC TCAGCAACAG TTACAGCTTG AGCAGATCCA ACAGCTGCAA 
CAACAGCTTC ACCAGCAGCT GGAGGAGCAA AAGATTCGGC AGATCTACCA GTATAACTAT 
GACCCTTCTG GAACTGCTTC TCCACAAACC ACTACAGAGC AGGCAATTTT GGAAGGTCAG 
TATGCTGCTC TGGAAGGCAG TCAATTTTGG GCAACTGAAG ATGCAACCAC CACAGCTTCA 
GCTGTTGTGG CAATTGAAAT ACCACAAAGC CAAGGATGGT ACACCGTTCA GTCTGATGGT 
GTTACTCAGT ACATTGCCCC ACCTGGTATC CTGAGCACTG TTTCAGAAAT ACCTCTAACA 
GATGTTGTTG TGAAAGAGGA AAAACAACCC AAAAAGAGAA GTTCTGGAGC TAAAGTCCGA 
GGACAGTATG ATGACATGGG AGAAAATATG ACAGATGATC CCCGAAGTTT TAAAAAGATA 
GTGGACAGTG GTGTACAAAC GGATGACGAA GATGCCACAG ATCGGAGCTA TGTGAGTAGG 
AGAAGGAGAA CTAAAAAGAG TGTGGATACA AGCGTCCAAA CTGATGATGA AGATCAGGAT 
GAGTGGGATA TGCCTACTAG ATCAAGGAGG AAAGCTCGTG TAGGGAAATA TGGTGACAGC 
ATGACAGAGG CTGACAAGAC CAAACCCCTT TCCAAAGTCT CCAGCATAGC AGTTCAAACG 
GTAGCAGAGA TATCTGTGCA AACTGAACCA GTTGGAACCA TAAGAACACC CTCCATACGG 
GCACGAGTGG ATGCCAAGGT AGAAATAATT AAACACATTT CAGCACCTGA AAAGACTTAC 
AAAGGGGGCA GTTTAGGATG TCAAACAGAA GCAGATTCAG ACACACAAAG TCCTCAATAT 
CTGAGTGCCA CATCTCCACC CAAAGACAAG AAACGCCCAA CACCTTTAGA GATTGGTTAT 
TCATCTCACC TCCGGGCAGA TTCCACAGTA CAGCTGGCTC CTTCCCCACC CAAATCCCCC 
AAAGTCCTTT ACTCACCCAT CTCACCACTT TCACCAGGCA AAGCCTTAGA ATCAGCCTTT 
GTACCTTATG AAAAACCCCT CCCTGATGAT ATAAGTCCAC AGAAAGTACT GCATCCAGAT 
ATGGCTAAAG TTCCCCCAGC AAGTCCTAAG ACAGCCAAGA TGATGCAGCG TTCTATGTCT 
GACCCCAAGC CTCTGAGTCC AACAGCAGAC GAAAGTTCCA GGGCTCCTTT TCAGTATACC 
GAGGGCTATA CGACTAAAGG TTCTCAAACC ATGACATCCT CTGGAGCCCA GAAAAAAGTT 
AAAAGAACTC TGCCAAATCC ACCTCCTGAG GAGATTTCCA CAGGAACTCA ATCCACATTC 
AGCACAATGG GCACAGTTTC CAGGAGAAGG ATCTGCAGAA CCAACACAAT GGCACGAGCC 
AAGATTCTCC AGGACATAGA CAGAGAGCTT GATCTTGTGG AAAGGGAGTC TGCAAAACTT 
CGAAAGAAAC AAGCAGAGCT TGATGAAGAA GAAAAGGAGA TTGATGCTAA GCTACGATAC 
CTGGAAATGG GAATTAACAG GAGGAAAGAG GCCCTATTAA AGGAGAGAGA AAAGAGAGAA 
CGAGCCTACC TCCAGGGAGT AGCTGAGGAT CGTGATTACA TGTCTGACAG TGAAGTGAGT 
AGCACAAGAC CAACCCGAAT AGAAAGTCAG CATGGCATTG AGCGACCAAG AACTGCTCCC 
CAAACTGAAT TCAGCCAGTT TATACCACCA CAAACCCAAA CAGAATCTCA ACTAGTTCCT 
CCGACAAGTC CTTACACACA ATACCAGTAC TCTTCCCCTG CTCTTCCTAC CCAAGCACCC 
ACCTCATACA CTCAACAGTC TCATTTTGAG CAACAAACTT TGTACCATCA GCAAGTTTCA 
CCTTATCAGA CTCAGCCAAC ATTCCAAGCT GTGGCAACAA TGTCCTTCAC ACCTCAAGTT 
CAACCTACAC CAACCCCACA GCCTTCTTAT CAGTTACCTT CACAGATGAT GGTGATACAA 
CAGAAGCCAC GGCAAACTAC ATTATATTTG GAGCCCAAGA TAACCTCAAA CTATGAAGTG 
ATTCGCAACC AACCCCTTAT GATAGCACCT GTTTCTACGG ATAACACATT TGCTGTTTCC 
CATCTTGGTA GTAAGTACAA TAGTTTAGAC TTGAGAATAG GTTTGGAGGA AAGAAGTAGC 
ATGGCAAGCA GTCCAATATC AAGCATATCT GCAGATTCTT TCTATGCAGA TATTGATCAC 
CATACTCCAC GAAATTATGT CCTAATTGAC GACATTGGAG AGATCACCAA AGGAACAGCG 
GCATTAAGCA CCGCATTTAG CCTTCATGAA AAGGATCTGT CAAAAACAGA CCGTCTCCTT 
CGAACCACTG AGACACGCCG GTCTCAAGAA GTGACAGATT TCCTAGCACC TTTACAGTCT 
TCCTCTAGAT TGCATAGTTA TGTGAAGGCG GAGGAAGACC CAATGGAGGA TCCTTACGAG 
TTAAAGCTTC TGAAACATCA GATTAAACAG GAATTTCGTA GAGGGACAGA GAGCTTAGAT 
CACCTTGCTG GTCTTTCTCA TTATTACCAT GCTGATACTA GCTACAGACA TTTTCCAAAA 
TCTGAGAAGT ATAGCATCAG TAGACTCACA CTTGAAAAAC AAGCAGCAAA ACAACTGCCA 
GCAGCCATAC TTTATCAAAA GCAGTCAAAG CATAAGAAAT CACTAATTGA CCCTAAAATG 
TCAAAATTTT CACCTATTCA AGAAAGTAGA GACCTTGAAC CTGATTATTC AAGCTATATG 
ACTTCTAGCA CTTCATCTAT TGGTGGCATT TCCTCCAGGG CAAGGCTCCT TCAAGATGAC 
ATCACTTTTG GCCTCAGAAA AAATATTACA GACCAACAAA AATTTATGGG ATCTTCTCTT 
GGCACAGGAC TGGGCACATT AGGAAATACC ATACGCTCAG CTCTGCAGGA TGAAGCGGAT 
AAGCCATACA GTAGTGGCAG CAGGTCCAGA CCTTCCTCCA GACCTTCCTC TGTCTATGGG 
CTTGATTTAT CAATTAAAAG GGATTCTTCT AGCTCTTCCC TAAGACTGAA AGCTCAAGAG 
GCTGAAGCTC TAGATGTTTC CTTTAGTCAT GCATCATCCT CTGCCAGAAC TAAGCCGACC 
AGTTTGCCAA TTAGTCAAAG TAGAGGAAGA ATACCAATTG TGGCCCAGAA TTCTGAAGAA 
GAAAGCCCAC TCAGTCCTGT TGGCCAGCCA ATGGGAATGG CCAGGGCTGC AGCTGGACCC 
CTGCCACCAA TATCTGCAGA CACCAGGGAT CAGTTTGGAT CAAGCCACTC ATTGCCTGAA 
GTTCAGCAAC ACATGAGGGA AGAATCACGG ACTCGAGGCT ATGACCGTGA CATAGCATTC 
ATCATGGATG ACTTCCAACA TGCCATGTCA GACAGTGAAG CCTATCATCT GCGTCGTGAG 
GAAACAGATT GGTTTGATAA ACCCAGGGAG TCTCGTTTGG AAAATGGACA TGGTCTGGAC 
CGAAAACTGC CGGAAAGATT GGTCCACTCT AGACCACTCA GTCAACATCA AGAGCAAATT 
ATACAGATGA ACGGGAAAAC TATGCACTAC ATCTTTCCTC ACGCAAGGAT AAAAATAACA 
AGAGACTCAA AGGATCACAC AGTTTCAGGT AATGGATTAG GAATTAGAAT TGTGGGTGGT 
AAAGAAATCC CGGGACATAG TGGAGAAATT GGAGCCTATA TTGCCAAGAT TCTTCCTGGG 
GGAAGTGCGG AACAGACGGG GAAGCTTATG GAAGGGATGC AAGTATTGGA ATGGAATGGA 
ATTCCCTTGA CTTCTAAAAC ATATGAAGAA GTTCAGAGTA TCATTAGTCA GCAAAGTGGG 
GAAGCAGAAA TATGTGTAAG ACTGGACCTC AATATGCTAT CAGATTCTGA AAATTCCCAG 
CATCTGGAAC TTCATGAGCC ACCAAAAGCT GTGGATAAGG CGAAATCCCC AGGGGTTGAT 
CCTAAGCAGT TGGCAGCAGA ACTCCAGAAG GTTTCACTAC AGCAGTCACC GCTGGTTCTG 
TCATCAGTTG TTGAAAAAGG ATCTCATGTT CATTCAGGTC CTACATCAGC AGGATCCAGT 
TCCGTTCCCA GCCCTGGGCA ACCAGGGTCC CCCTCAGTGA GCAAAAAGAA GCACGGCAGC 
AGCAAGCCTA CCGATGGAAC AAAGGTTGTC TCTCATCCAA TTACAGGAGA AATTCAGCTT 
CAAATTAACT ATGATCTTGG AAATCTCATA ATACATATTC TCCAAGCAAG AAATCTTGTT 
CCTCGAGACA ACAATGGTTA TTCTGACCCT TTTGTGAAAG TGTACCTTCT TCCAGGGAGA 
GGTCAAGTCA TGGTTGTCCA GAATGCAAGT GCTGAGTACA AGAGAAGGAC TAAACATGTC 
CAGAAAAGTC TTAATCCTGA GTGGAATCAA ACAGTAATTT ATAAAAGTAT TTCCATGGAA 
CAGCTCAAGA AGAAAACACT GGAGGTGACA GTTTGGGATT ATGATAGATT TTCATCCAAC 
GACTTCCTTG GGGAGGTATT GATTGATTTA TCTAGCACAT CTCACCTCGA TAACACTCCA 
AGGTGGTATC CTCTCAAAGA ACAGACTGAA AGCATTGATC ATGGCAAGTC TCATTCCAGT 
CAGAGCAGCC AGCAGTCCCC AAAGCCATCT GTTATCAAAA GCAGAAGCCA TGGTATCTTC 
CCTGACCCAT CAAAGGACAT GCAGGTTCCC ACCATTGAGA AATCCCATAG TAGTCCTGGT 
AGCTCAAAAT CATCATCAGA AGGCCATCTC CGTTCTCATG GACCATCTCG CAGTCAAAGC 
AAAACCAGCG TCACTCAGAC CCACCTGGAA GATGCAGGGG CTGCCATAGC TGCTGCCGAA 
GCTGCCGTGC AACAACTCCG CATTCAACCA AGTAAAAGAC GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055325.2
CDS	290..15097
Translation	MGNEASLEGEGLPEGLAAAAAAGGGASGAGSPSHTAIPAGMEADLSQLSEEERRQIAAVMSRAQGLPKGSVPPAAAESPSMHRKQELDSSHPPKQSGRPPDPGRPAQPGLSKSRTTDTFRSEQKLPGRSPSTISLKESKSRTDLKEEHKSSMMPGFLSEVNALSAVSSVVNKFNPFDLISDSEASQEETTKKQKVVQKEQGKPEGIIKPPLQQQPPKPIPKQQGPGRDPLQQDGTPKSISSQQPEKIKSQPPGTGKPIQGPTQTPQTDHAKLPLQRDASRPQTKQADIVRGESVKPSLPSPSKPPIQQPTPGKPPAQQPGHEKSQPGPAKPPAQPSGLTKPLAQQPGTVKPPVQPPGTTKPPAQPLGPAKPPAQQTGSEKPSSEQPGPKALAQPPGVGKTPAQQPGPAKPPTQQVGTPKPLAQQPGLQSPAKAPGPTKTPVQQPGPGKIPAQQAGPGKTSAQQTGPTKPPSQLPGPAKPPPQQPGPAKPPPQQPGSAKPPSQQPGSTKPPPQQPGPAKPSPQQPGSTKPPSQQPGSAKPSAQQPSPAKPSAQQSTKPVSQTGSGKPLQPPTVSPSAKQPPSQGLPKTICPLCNTTELLLHVPEKANFNTCTECQTTVCSLCGFNPNPHLTEVKEWLCLNCQMKRALGGDLAPVPSSPQPKLKTAPVTTTSAVSKSSPQPQQTSPKKDAAPKQDLSKAPEPKKPPPLVKQPTLHGSPSAKAKQPPEADSLSKPAPPKEPSVPSEQDKAPVADDKPKQPKMVKPTTDLVSSSSATTKPDIPSSKVQSQAEEKTTPPLKTDSAKPSQSFPPTGEKVSPFDSKAIPRPASDSKIISHPGPSSESKGQKQVDPVQKKEEPKKAQTKMSPKPDAKPMPKGSPTPPGPRPTAGQTVPTPQQSPKPQEQSRRFSLNLGSITDAPKSQPTTPQETVTGKLFGFGASIFSQASNLISTAGQPGPHSQSGPGAPMKQAPAPSQPPTSQGPPKSTGQAPPAPAKSIPVKKETKAPAAEKLEPKAEQAPTVKRTETEKKPPPIKDSKSLTAEPQKAVLPTKLEKSPKPESTCPLCKTELNIGSKDPPNFNTCTECKNQVCNLCGFNPTPHLTEIQEWLCLNCQTQRAISGQLGDIRKMPPAPSGPKASPMPVPTESSSQKTAVPPQVKLVKKQEQEVKTEAEKVILEKVKETLSMEKIPPMVTTDQKQEESKLEKDKASALQEKKPLPEEKKLIPEEEKIRSEEKKPLLEEKKPTPEDKKLLPEAKTSAPEEQKHDLLKSQVQIAEEKLEGRVAPKTVQEGKQPQTKMEGLPSGTPQSLPKEDDKTTKTIKEQPQPPCTAKPDQVEPGKEKTEKEDDKSDTSSSQQPKSPQGLSDTGYSSDGISSSLGEIPSLIPTDEKDILKGLKKDSFSQESSPSSPSDLAKLESTVLSILEAQASTLADEKSEKKTQPHEVSPEQPKDQEKTQSLSETLEITISEEEIKESQEERKDTFKKDSQQDIPSSKDHKEKSEFVDDITTRREPYDSVEESSESENSPVPQRKRRTSVGSSSSDEYKQEDSQGSGEEEDFIRKQIIEMSADEDASGSEDDEFIRNQLKEISSSTESQKKEETKGKGKITAGKHRRLTRKSSTSIDEDAGRRHSWHDEDDEAFDESPELKYRETKSQESEELVVTGGGGLRRFKTIELNSTIADKYSAESSQKKTSLYFDEEPELEMESLTDSPEDRSRGEGSSSLHASSFTPGTSPTSVSSLDEDSDSSPSHKKGESKQQRKARHRPHGPLLPTIEDSSEEEELREEEELLKEQEKQREIEQQQRKSSSKKSKKDKDELRAQRRRERPKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSPSIESDPEGFEISPEKIIEVQKVYKLPTAVSLYSPTDEQSIMQKEGSQKALKSAEEMYEEMMHKTHKYKAFPAANERDEVFEKEPLYGGMLIEDYIYESLVEDTYNGSVDGSLLTRQEEENGFMQQKGREQKIRLSEQIYEDPMQKITDLQKEFYELESLHSVVPQEDIVSSSFIIPESHEIVDLGTMVTSTEEERKLLDADAAYEELMKRQQMQLTPGSSPTQAPIGEDMTESTMDFDRMPDASLTSSVLSGASLTDSTSSATLSIPDVKITQHFSTEEIEDEYVTDYTREIQEIIAHESLILTYSEPSESATSVPPSDTPSLTSSVSSVCTTDSSSPITTLDSITTVYTEPVDMITKFEDSEEISSSTYFPGSIIDYPEEISVSLDRTAPPDGRASADHIVISLSDMASSIIESVVPKPEGPVADTVSTDLLISEKDPVKKAKKETGNGIILEVLEAYRDKKELEAERTKSSLSETVFDHPPSSVIALPMKEQLSTTYFTSGETFGQEKPASQLPSGSPSVSSLPAKPRPFFRSSSLDISAQPPPPPPPPPPPPPPPPPPPPPPLPPPTSPKPTILPKKKLTVASPVTTATPLFDAVTTLETTAVLRSNGLPVTRICTTAPPPVPPKPSSIPSGLVFTHRPEPSKPPIAPKPVIPQLPTTTQKPTDIHPKPTGLSLTSSMTLNLVTSADYKLPSPTSPLSPHSNKSSPRFSKSLTETYVVITLPSEPGTPTDSSASQAITSWPLGSPSKDLVSVEPVFSVVPPVTAVEIPISSEQTFYISGALQTFSATPVTAPSSFQAAPTSVTQFLTTEVSKTEVSATRSTAPSVGLSSISITIPPEPLALDNIHLEKPQYKEDGKLQLVGDVIDLRTVPKVEVKTTDKCIDLSASTMDVKRQITANEVYGKQISAVQPSIINLSVTSSIVTPVSLATETVTFVTCTASASYTTGTESLVGAEHAMTTPLQLTTSKHAEPPYRIPSDQVFPIAREEAPINLSLGTPAHAVTLAITKPVTVPPVGVTNGWTDSTVSQGITDGEVVDLSTTKSHRTVVTMDESTSSVMTKIIEDEKPVDLTAGRRAVCCDVVYKLPFGRSCTAQQPATTLPEDRFGYRDDHYQYDRSGPYGYRGIGGMKPSMSDTNLAEAGHFFYKSKNAFDYSEGTDTAVDLTSGRVTTGEVMDYSSKTTGPYPETRQVISGAGISTPQYSTARMTPPPGPQYCVGSVLRSSNGVVYSSVATPTPSTFAITTQPGSIFSTTVRDLSGIHTADAVTSLPAMHHSQPMPRSYFITTGASETDIAVTGIDISASLQTITMESLTAETIDSVPTLTTASEVFPEVVGDESALLIVPEEDKQQQQLDLERELLELEKIKQQRFAEELEWERQEIQRFREQEKIMVQKKLEELQSMKQHLLFQQEEERQAQFMMRQETLAQQQLQLEQIQQLQQQLHQQLEEQKIRQIYQYNYDPSGTASPQTTTEQAILEGQYAALEGSQFWATEDATTTASAVVAIEIPQSQGWYTVQSDGVTQYIAPPGILSTVSEIPLTDVVVKEEKQPKKRSSGAKVRGQYDDMGENMTDDPRSFKKIVDSGVQTDDEDATDRSYVSRRRRTKKSVDTSVQTDDEDQDEWDMPTRSRRKARVGKYGDSMTEADKTKPLSKVSSIAVQTVAEISVQTEPVGTIRTPSIRARVDAKVEIIKHISAPEKTYKGGSLGCQTEADSDTQSPQYLSATSPPKDKKRPTPLEIGYSSHLRADSTVQLAPSPPKSPKVLYSPISPLSPGKALESAFVPYEKPLPDDISPQKVLHPDMAKVPPASPKTAKMMQRSMSDPKPLSPTADESSRAPFQYTEGYTTKGSQTMTSSGAQKKVKRTLPNPPPEEISTGTQSTFSTMGTVSRRRICRTNTMARAKILQDIDRELDLVERESAKLRKKQAELDEEEKEIDAKLRYLEMGINRRKEALLKEREKRERAYLQGVAEDRDYMSDSEVSSTRPTRIESQHGIERPRTAPQTEFSQFIPPQTQTESQLVPPTSPYTQYQYSSPALPTQAPTSYTQQSHFEQQTLYHQQVSPYQTQPTFQAVATMSFTPQVQPTPTPQPSYQLPSQMMVIQQKPRQTTLYLEPKITSNYEVIRNQPLMIAPVSTDNTFAVSHLGSKYNSLDLRIGLEERSSMASSPISSISADSFYADIDHHTPRNYVLIDDIGEITKGTAALSTAFSLHEKDLSKTDRLLRTTETRRSQEVTDFLAPLQSSSRLHSYVKAEEDPMEDPYELKLLKHQIKQEFRRGTESLDHLAGLSHYYHADTSYRHFPKSEKYSISRLTLEKQAAKQLPAAILYQKQSKHKKSLIDPKMSKFSPIQESRDLEPDYSSYMTSSTSSIGGISSRARLLQDDITFGLRKNITDQQKFMGSSLGTGLGTLGNTIRSALQDEADKPYSSGSRSRPSSRPSSVYGLDLSIKRDSSSSSLRLKAQEAEALDVSFSHASSSARTKPTSLPISQSRGRIPIVAQNSEEESPLSPVGQPMGMARAAAGPLPPISADTRDQFGSSHSLPEVQQHMREESRTRGYDRDIAFIMDDFQHAMSDSEAYHLRREETDWFDKPRESRLENGHGLDRKLPERLVHSRPLSQHQEQIIQMNGKTMHYIFPHARIKITRDSKDHTVSGNGLGIRIVGGKEIPGHSGEIGAYIAKILPGGSAEQTGKLMEGMQVLEWNGIPLTSKTYEEVQSIISQQSGEAEICVRLDLNMLSDSENSQHLELHEPPKAVDKAKSPGVDPKQLAAELQKVSLQQSPLVLSSVVEKGSHVHSGPTSAGSSSVPSPGQPGSPSVSKKKHGSSKPTDGTKVVSHPITGEIQLQINYDLGNLIIHILQARNLVPRDNNGYSDPFVKVYLLPGRGQVMVVQNASAEYKRRTKHVQKSLNPEWNQTVIYKSISMEQLKKKTLEVTVWDYDRFSSNDFLGEVLIDLSSTSHLDNTPRWYPLKEQTESIDHGKSHSSQSSQQSPKPSVIKSRSHGIFPDPSKDMQVPTIEKSHSSPGSSKSSSEGHLRSHGPSRSQSKTSVTQTHLEDAGAAIAAAEAAVQQLRIQPSKRRK

Target ORF information:

RefSeq Version	NM_014510.2
Organism	Homo sapiens(human)
Definition	Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014510.2

ORF Insert Sequence:

ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA 
GCGGCTGGAG GAGGAGCTAG CGGGGCGGGG AGCCCCTCTC ACACCGCGAT CCCGGCCGGC 
ATGGAGGCGG ATTTGAGCCA GCTGAGCGAA GAGGAGAGGA GACAGATCGC CGCTGTCATG 
TCAAGGGCGC AGGGGCTGCC CAAGGGAAGC GTCCCCCCGG CCGCTGCGGA GTCGCCTTCC 
ATGCACAGGA AACAAGAGTT GGATAGTAGT CATCCTCCAA AGCAATCAGG AAGACCCCCG 
GACCCTGGGC GTCCAGCTCA ACCTGGTCTC AGTAAAAGTA GAACTACAGA CACTTTCAGG 
TCAGAGCAGA AATTGCCTGG GAGGAGTCCT TCCACTATTA GCTTGAAAGA ATCAAAGTCC 
AGAACTGATT TAAAGGAAGA GCACAAGTCT AGTATGATGC CTGGCTTCCT CTCAGAGGTT 
AACGCTTTAA GTGCTGTTTC CTCTGTTGTA AATAAATTCA ACCCTTTTGA TTTGATATCA 
GACTCTGAGG CATCCCAGGA AGAAACCACC AAGAAACAAA AAGTGGTTCA GAAGGAGCAA 
GGAAAACCTG AAGGAATCAT AAAACCTCCT TTACAACAAC AGCCACCCAA GCCGATTCCT 
AAGCAGCAAG GACCTGGTAG GGATCCGCTT CAGCAGGATG GCACTCCCAA ATCAATATCT 
TCTCAACAAC CAGAAAAAAT TAAATCACAA CCTCCAGGTA CAGGAAAGCC AATTCAGGGT 
CCTACCCAGA CTCCTCAGAC AGACCATGCA AAATTGCCAC TTCAACGAGA TGCATCCAGG 
CCTCAGACTA AACAGGCAGA CATAGTAAGG GGAGAATCAG TTAAACCCTC ACTGCCAAGC 
CCATCCAAAC CACCTATTCA GCAACCAACT CCTGGAAAAC CTCCAGCACA GCAGCCTGGA 
CATGAAAAAT CACAGCCTGG GCCTGCAAAG CCCCCAGCTC AGCCCTCAGG GCTAACAAAG 
CCATTGGCTC AACAACCAGG GACAGTGAAA CCCCCAGTCC AGCCACCAGG GACAACAAAG 
CCTCCAGCTC AGCCTCTTGG TCCTGCTAAG CCTCCAGCTC AGCAGACTGG GTCAGAGAAG 
CCTTCATCGG AGCAGCCTGG GCCAAAGGCT TTAGCTCAGC CTCCTGGAGT TGGAAAGACT 
CCAGCTCAAC AGCCAGGGCC AGCAAAGCCT CCAACCCAGC AGGTGGGGAC ACCAAAACCC 
CTAGCTCAAC AACCTGGGCT ACAGTCTCCA GCTAAGGCAC CTGGGCCTAC AAAGACTCCA 
GTTCAGCAGC CTGGGCCAGG AAAGATTCCA GCTCAACAGG CAGGACCCGG AAAGACTTCT 
GCCCAGCAGA CTGGCCCAAC AAAGCCTCCT TCACAACTGC CTGGCCCAGC AAAGCCCCCA 
CCTCAACAGC CTGGCCCAGC AAAGCCCCCA CCTCAACAGC CTGGCTCAGC AAAGCCCCCA 
TCTCAACAGC CTGGCTCAAC AAAACCCCCA CCTCAACAGC CTGGCCCAGC AAAGCCCTCA 
CCTCAACAGC CTGGCTCAAC AAAACCCCCA TCTCAACAGC CTGGCTCAGC AAAACCCTCA 
GCTCAACAGC CTAGCCCAGC AAAGCCCTCG GCTCAGCAAT CTACAAAACC AGTAAGCCAA 
ACAGGATCTG GAAAACCTCT GCAGCCACCA ACAGTGTCTC CATCTGCAAA ACAGCCTCCT 
TCACAAGGCC TCCCTAAAAC CATCTGTCCT CTTTGCAATA CCACTGAACT TCTGTTGCAT 
GTTCCAGAAA AGGCCAATTT TAACACATGC ACTGAGTGTC AAACCACTGT CTGTAGTCTC 
TGTGGTTTTA ATCCCAATCC TCATTTAACG GAGGTAAAAG AGTGGCTCTG TTTGAACTGT 
CAAATGAAAA GAGCTCTAGG CGGGGATCTG GCTCCAGTTC CGTCATCACC CCAGCCCAAA 
CTGAAGACTG CACCTGTTAC CACTACATCA GCAGTGAGCA AATCATCCCC ACAGCCACAG 
CAGACTTCCC CAAAGAAGGA TGCTGCACCA AAACAGGATC TCTCCAAGGC ACCTGAGCCT 
AAAAAGCCAC CACCACTAGT GAAACAACCA ACCCTTCATG GCTCTCCTTC AGCCAAGGCC 
AAGCAGCCCC CTGAGGCAGA TTCTTTGTCC AAGCCAGCCC CTCCCAAAGA ACCTTCTGTC 
CCATCTGAGC AGGACAAGGC CCCTGTTGCT GATGATAAAC CAAAGCAGCC CAAGATGGTA 
AAGCCAACCA CTGACCTTGT ATCTTCATCA TCAGCAACAA CAAAACCTGA TATTCCAAGC 
TCCAAAGTAC AGTCACAAGC TGAAGAGAAA ACAACCCCTC CTCTAAAAAC AGACTCTGCC 
AAACCCTCAC AGAGTTTTCC ACCAACAGGG GAAAAAGTCA GCCCATTTGA TTCTAAAGCC 
ATACCTCGAC CTGCATCAGA TTCAAAAATT ATTTCACATC CTGGTCCCAG TTCAGAGAGC 
AAAGGTCAAA AACAAGTTGA CCCCGTACAA AAGAAGGAAG AACCCAAGAA AGCACAAACC 
AAAATGAGTC CTAAACCAGA TGCCAAGCCA ATGCCAAAAG GGTCACCAAC ACCCCCTGGC 
CCACGACCTA CCGCTGGCCA AACTGTCCCC ACACCTCAAC AGTCCCCAAA GCCTCAGGAG 
CAGTCAAGGC GTTTCAGTCT GAATCTGGGA AGTATTACTG ATGCCCCCAA ATCACAGCCT 
ACAACTCCTC AAGAGACCGT GACTGGGAAA CTCTTTGGGT TTGGAGCATC AATCTTCAGC 
CAGGCATCAA ATTTAATTTC CACTGCAGGC CAACCTGGAC CTCATTCACA AAGTGGACCA 
GGGGCCCCAA TGAAACAAGC CCCTGCCCCT TCACAGCCAC CTACTTCACA AGGGCCACCC 
AAATCCACAG GTCAAGCACC ACCAGCACCT GCAAAAAGTA TACCTGTGAA AAAGGAAACA 
AAAGCCCCAG CAGCTGAAAA ATTAGAGCCC AAAGCTGAAC AAGCTCCAAC AGTAAAAAGA 
ACAGAAACAG AAAAAAAGCC ACCACCTATT AAGGATAGCA AATCTTTAAC AGCTGAGCCT 
CAAAAGGCTG TCCTTCCCAC AAAACTGGAG AAATCGCCCA AACCAGAATC AACCTGTCCT 
CTCTGCAAAA CTGAACTCAA CATAGGTTCT AAGGATCCTC CTAACTTCAA TACTTGCACT 
GAATGCAAGA ATCAAGTGTG TAATCTCTGT GGATTTAACC CTACACCACA TTTGACTGAG 
ATTCAAGAAT GGCTTTGTTT AAATTGCCAA ACCCAGAGAG CAATATCAGG ACAGCTTGGA 
GACATACGCA AAATGCCACC TGCACCATCA GGACCCAAAG CATCTCCTAT GCCTGTTCCT 
ACAGAATCAT CATCTCAGAA AACAGCAGTG CCTCCCCAAG TAAAATTAGT GAAAAAGCAA 
GAACAAGAAG TAAAAACGGA AGCTGAAAAA GTCATTCTGG AAAAAGTAAA GGAAACACTA 
TCAATGGAAA AAATTCCTCC TATGGTAACC ACAGATCAAA AACAAGAAGA GAGTAAACTA 
GAGAAAGACA AAGCTTCAGC TCTTCAAGAA AAAAAGCCAC TCCCTGAAGA AAAAAAACTA 
ATCCCTGAAG AAGAAAAGAT ACGTTCTGAA GAAAAAAAGC CACTCCTAGA AGAAAAAAAG 
CCAACCCCTG AAGACAAAAA GCTACTCCCA GAGGCAAAAA CATCAGCCCC AGAAGAACAG 
AAACATGACT TACTTAAATC TCAAGTACAA ATTGCTGAAG AAAAGCTTGA AGGCAGAGTG 
GCTCCAAAGA CAGTGCAAGA AGGGAAACAA CCACAGACCA AGATGGAAGG TTTACCATCT 
GGCACACCTC AGAGTTTACC TAAAGAAGAT GATAAGACAA CCAAAACAAT AAAAGAACAG 
CCACAGCCAC CATGCACAGC AAAACCTGAT CAGGTGGAAC CTGGGAAAGA AAAAACAGAA 
AAGGAAGATG ACAAATCAGA CACCTCAAGT TCTCAGCAGC CTAAAAGCCC CCAAGGTCTG 
AGCGACACGG GATATTCTTC CGATGGAATA TCAAGCTCAC TTGGTGAAAT TCCAAGTCTT 
ATTCCAACTG ATGAAAAGGA TATTCTCAAG GGACTCAAAA AGGACTCTTT TTCACAAGAA 
AGCAGCCCTT CCAGCCCCTC AGATTTGGCT AAGTTAGAAA GTACAGTCCT ATCTATTTTG 
GAAGCTCAAG CAAGTACACT TGCTGATGAA AAGTCAGAAA AGAAAACACA ACCCCATGAA 
GTTTCTCCTG AACAGCCTAA AGACCAAGAG AAAACTCAGA GTTTATCTGA AACCTTGGAA 
ATTACTATTT CAGAAGAGGA GATCAAAGAG AGTCAAGAAG AAAGGAAAGA CACTTTTAAA 
AAAGATAGCC AACAAGATAT TCCTTCCAGC AAGGACCATA AAGAGAAGTC TGAGTTTGTT 
GATGACATAA CTACTAGAAG AGAGCCTTAT GATTCAGTTG AAGAGAGTAG TGAAAGTGAA 
AACTCACCTG TTCCACAAAG AAAACGAAGA ACTAGTGTTG GCTCATCAAG CAGTGATGAG 
TATAAACAGG AAGACAGCCA AGGATCAGGG GAAGAGGAGG ACTTCATTCG AAAACAAATC 
ATAGAAATGA GTGCTGATGA AGATGCTTCA GGTTCTGAAG ATGATGAGTT CATCAGAAAC 
CAGCTCAAAG AGATTAGTAG CAGTACTGAG AGCCAGAAGA AGGAAGAAAC AAAGGGAAAA 
GGCAAAATAA CAGCAGGGAA ACACAGACGA CTGACTCGAA AAAGTAGCAC AAGCATTGAT 
GAAGATGCAG GAAGACGTCA CTCATGGCAT GATGAAGACG ATGAAGCATT TGATGAAAGT 
CCTGAACTTA AATACAGAGA AACTAAAAGT CAGGAAAGTG AAGAACTTGT AGTTACTGGA 
GGAGGAGGGC TACGCCGATT TAAAACAATT GAGCTCAACA GTACAATAGC AGATAAATAT 
TCTGCAGAGT CATCACAGAA AAAAACAAGT TTGTATTTTG ACGAAGAGCC AGAATTGGAA 
ATGGAAAGCC TGACAGACTC ACCTGAAGAT AGGTCAAGGG GAGAGGGATC TTCGAGTCTG 
CATGCTTCCA GCTTCACTCC TGGTACATCC CCTACATCAG TATCATCACT TGATGAGGAC 
AGTGACAGTA GCCCGAGTCA CAAAAAAGGA GAGAGCAAAC AGCAACGCAA AGCTCGGCAC 
AGACCACATG GCCCTCTTTT GCCTACTATT GAAGATTCTT CAGAGGAAGA AGAATTGAGA 
GAGGAAGAAG AATTATTAAA GGAGCAAGAA AAGCAGAGGG AAATAGAACA GCAACAAAGA 
AAGAGTTCTA GTAAAAAATC AAAGAAAGAC AAAGATGAAC TTCGAGCTCA GAGAAGAAGG 
GAAAGGCCAA AGACACCACC TAGTAATCTC TCTCCCATTG AAGATGCATC TCCGACAGAA 
GAGTTACGTC AGGCTGCAGA AATGGAGGAG CTCCATAGAT CTTCTTGTTC TGAATATTCA 
CCTAGCATAG AGTCAGACCC AGAAGGTTTT GAAATAAGCC CGGAAAAAAT AATAGAAGTA 
CAAAAAGTTT ATAAATTGCC CACAGCTGTT TCATTATACT CACCAACAGA TGAGCAATCT 
ATTATGCAGA AAGAAGGTAG CCAAAAGGCG TTAAAAAGTG CTGAGGAGAT GTATGAAGAA 
ATGATGCATA AAACACACAA ATACAAAGCT TTTCCAGCTG CAAATGAACG AGATGAAGTG 
TTTGAAAAAG AGCCTTTGTA TGGTGGGATG CTAATAGAGG ATTATATTTA TGAATCTTTA 
GTAGAAGACA CGTACAATGG ATCGGTAGAT GGCAGTCTGC TAACAAGGCA AGAAGAAGAA 
AATGGATTTA TGCAGCAGAA AGGAAGAGAG CAAAAGATAA GACTTTCAGA ACAGATTTAT 
GAAGATCCTA TGCAGAAAAT TACAGACCTC CAGAAAGAGT TTTATGAGTT AGAAAGCTTA 
CATTCTGTTG TGCCTCAGGA AGATATTGTT TCAAGCTCTT TTATCATCCC AGAAAGCCAT 
GAGATAGTGG ACCTGGGTAC TATGGTAACT TCTACAGAAG AAGAAAGGAA ACTACTAGAT 
GCTGATGCTG CCTATGAAGA ACTTATGAAG AGGCAACAGA TGCAATTAAC ACCTGGATCT 
AGCCCAACCC AGGCCCCCAT TGGTGAGGAT ATGACAGAGT CCACCATGGA CTTTGACAGA 
ATGCCAGATG CCTCTTTGAC ATCAAGTGTT CTCTCAGGAG CGTCTCTTAC AGATTCGACC 
AGCAGTGCAA CACTCTCTAT CCCAGATGTT AAAATAACCC AACATTTTTC AACAGAAGAA 
ATTGAGGATG AATATGTAAC CGATTATACA AGAGAAATTC AAGAGATAAT TGCCCATGAA 
TCGCTGATTT TGACCTACTC GGAGCCTTCA GAAAGTGCTA CATCTGTCCC ACCCTCTGAC 
ACACCTTCTC TCACATCATC TGTTTCTTCG GTCTGTACCA CAGATAGCTC TTCACCCATT 
ACTACCCTGG ATAGCATAAC CACAGTTTAT ACAGAGCCAG TGGACATGAT AACTAAATTT 
GAAGATTCTG AGGAAATTTC TTCATCAACT TATTTTCCAG GCAGCATTAT AGACTATCCA 
GAAGAAATAA GTGTATCTTT AGATCGGACT GCCCCACCAG ATGGTAGAGC TAGTGCTGAT 
CATATTGTTA TTTCCTTATC TGATATGGCA TCTTCTATCA TAGAATCTGT AGTACCTAAA 
CCTGAAGGGC CAGTTGCTGA CACTGTTTCT ACTGACTTAC TTATATCTGA AAAGGACCCA 
GTGAAGAAAG CCAAGAAGGA AACTGGGAAT GGAATCATTC TGGAAGTTTT GGAAGCTTAC 
AGAGATAAAA AGGAGTTGGA GGCCGAACGA ACAAAAAGTA GCTTATCCGA AACCGTGTTT 
GATCACCCAC CTTCTTCTGT AATAGCCCTT CCAATGAAAG AGCAGCTTTC AACTACATAC 
TTTACATCTG GAGAGACCTT TGGTCAGGAA AAACCTGCAT CTCAGTTACC ATCTGGCAGT 
CCTTCTGTTT CCTCTCTTCC AGCTAAACCT CGCCCATTCT TTAGAAGTTC TTCTTTGGAT 
ATATCAGCTC AACCTCCTCC CCCTCCTCCC CCTCCCCCTC CTCCTCCTCC TCCACCACCA 
CCCCCTCCTC CCCCACCACT TCCTCCACCA ACTTCACCTA AACCAACTAT TCTTCCTAAA 
AAAAAGTTAA CAGTTGCATC TCCAGTGACT ACAGCTACAC CTCTGTTTGA TGCTGTTACT 
ACTCTAGAGA CCACAGCTGT TCTGAGAAGT AATGGATTAC CTGTTACAAG AATATGTACT 
ACTGCACCTC CTCCTGTTCC TCCTAAGCCA TCTTCAATTC CATCTGGACT TGTATTTACC 
CACAGGCCTG AGCCAAGCAA ACCTCCAATC GCCCCCAAAC CAGTGATTCC TCAGCTTCCA 
ACAACTACAC AAAAACCAAC AGATATACAC CCCAAACCAA CAGGCCTATC TTTAACTTCA 
AGTATGACCT TAAATTTAGT GACTTCAGCA GATTATAAAT TGCCTTCCCC TACCTCCCCA 
CTTTCCCCAC ACTCCAACAA GTCTTCACCA AGATTTTCCA AATCCCTCAC AGAAACTTAT 
GTAGTTATTA CATTGCCATC TGAACCAGGG ACTCCAACAG ATTCTTCTGC TAGTCAAGCA 
ATTACCAGTT GGCCCTTGGG ATCACCCTCC AAAGATCTGG TTTCTGTTGA ACCTGTGTTT 
TCTGTAGTTC CTCCTGTGAC AGCTGTAGAA ATTCCAATTT CTTCAGAACA GACCTTCTAC 
ATCTCTGGAG CTTTACAGAC ATTTTCTGCT ACCCCTGTCA CAGCACCCTC TTCATTTCAA 
GCAGCTCCCA CATCAGTTAC ACAGTTTCTC ACTACTGAAG TTTCCAAGAC TGAGGTTTCA 
GCAACCAGAA GTACAGCTCC TAGTGTTGGT CTCAGCAGCA TTTCCATAAC AATTCCTCCA 
GAGCCTCTTG CTCTAGATAA CATACATTTA GAGAAGCCTC AGTATAAAGA AGATGGAAAA 
TTGCAACTTG TTGGTGATGT AATTGATTTG CGTACAGTAC CAAAGGTAGA AGTTAAAACA 
ACTGATAAAT GTATTGATCT TTCTGCTTCT ACAATGGATG TGAAAAGGCA GATCACAGCA 
AATGAAGTTT ATGGGAAACA AATTAGTGCT GTCCAACCCT CTATTATAAA TCTTAGTGTG 
ACATCATCAA TAGTGACTCC TGTATCTCTG GCCACTGAGA CAGTGACCTT TGTCACATGC 
ACAGCTAGTG CAAGTTACAC TACAGGCACA GAAAGCCTAG TGGGTGCAGA ACATGCAATG 
ACAACACCAC TCCAACTTAC AACATCAAAG CATGCTGAGC CCCCATACAG GATACCAAGT 
GACCAGGTCT TTCCTATAGC TAGGGAAGAA GCACCAATAA ACTTATCTCT AGGTACTCCA 
GCACATGCAG TGACATTGGC TATTACAAAA CCTGTCACTG TGCCTCCTGT TGGTGTCACA 
AATGGATGGA CTGATAGCAC CGTATCCCAG GGAATCACTG ATGGGGAAGT AGTGGATCTC 
AGTACAACCA AGTCTCACAG AACAGTCGTA ACAATGGATG AGTCTACTTC AAGTGTGATG 
ACCAAAATAA TAGAAGATGA AAAACCCGTT GATTTAACCG CAGGGAGAAG AGCTGTGTGC 
TGTGATGTGG TTTATAAATT ACCATTTGGA AGGAGCTGCA CAGCACAGCA GCCTGCAACT 
ACTCTTCCTG AGGATCGTTT TGGTTATAGG GATGACCACT ATCAGTATGA TCGATCAGGG 
CCATATGGTT ATAGAGGGAT TGGGGGAATG AAGCCTTCCA TGTCTGACAC AAATTTAGCA 
GAAGCTGGAC ATTTTTTCTA TAAAAGTAAG AATGCTTTTG ATTATTCTGA AGGAACTGAC 
ACAGCAGTAG ATCTGACTTC AGGGAGAGTT ACTACAGGTG AGGTAATGGA TTATTCAAGC 
AAGACTACAG GTCCATATCC AGAAACACGA CAAGTCATTT CAGGAGCTGG GATTAGTACC 
CCACAGTATT CCACAGCAAG AATGACACCA CCACCAGGAC CCCAGTATTG TGTGGGGAGT 
GTTTTGAGGT CATCTAATGG TGTTGTCTAT TCTTCAGTAG CAACTCCAAC ACCCTCTACA 
TTTGCTATCA CCACACAACC TGGCTCCATT TTCAGCACCA CAGTGAGGGA TTTGTCTGGT 
ATTCATACGG CTGATGCAGT GACTTCATTA CCTGCCATGC ACCATAGCCA GCCAATGCCT 
AGATCATATT TTATAACAAC AGGTGCATCT GAAACGGACA TTGCAGTAAC TGGTATTGAT 
ATCAGTGCCA GTTTGCAAAC TATTACTATG GAGTCTCTTA CTGCTGAGAC GATAGACTCT 
GTTCCCACTT TAACCACAGC ATCCGAAGTG TTTCCTGAAG TGGTGGGAGA TGAAAGTGCT 
CTTTTAATTG TCCCTGAAGA AGATAAACAA CAGCAGCAGC TAGACTTGGA GCGTGAGCTC 
CTGGAACTGG AGAAAATTAA GCAACAGCGC TTTGCTGAGG AATTGGAGTG GGAACGTCAG 
GAAATTCAAA GGTTCCGAGA ACAAGAAAAG ATCATGGTTC AGAAAAAGTT GGAGGAGCTG 
CAGTCTATGA AGCAACACCT TCTCTTTCAG CAAGAAGAAG AGCGGCAAGC CCAGTTCATG 
ATGAGGCAGG AGACGTTAGC TCAGCAACAG TTACAGCTTG AGCAGATCCA ACAGCTGCAA 
CAACAGCTTC ACCAGCAGCT GGAGGAGCAA AAGATTCGGC AGATCTACCA GTATAACTAT 
GACCCTTCTG GAACTGCTTC TCCACAAACC ACTACAGAGC AGGCAATTTT GGAAGGTCAG 
TATGCTGCTC TGGAAGGCAG TCAATTTTGG GCAACTGAAG ATGCAACCAC CACAGCTTCA 
GCTGTTGTGG CAATTGAAAT ACCACAAAGC CAAGGATGGT ACACCGTTCA GTCTGATGGT 
GTTACTCAGT ACATTGCCCC ACCTGGTATC CTGAGCACTG TTTCAGAAAT ACCTCTAACA 
GATGTTGTTG TGAAAGAGGA AAAACAACCC AAAAAGAGAA GTTCTGGAGC TAAAGTCCGA 
GGACAGTATG ATGACATGGG AGAAAATATG ACAGATGATC CCCGAAGTTT TAAAAAGATA 
GTGGACAGTG GTGTACAAAC GGATGACGAA GATGCCACAG ATCGGAGCTA TGTGAGTAGG 
AGAAGGAGAA CTAAAAAGAG TGTGGATACA AGCGTCCAAA CTGATGATGA AGATCAGGAT 
GAGTGGGATA TGCCTACTAG ATCAAGGAGG AAAGCTCGTG TAGGGAAATA TGGTGACAGC 
ATGACAGAGG CTGACAAGAC CAAACCCCTT TCCAAAGTCT CCAGCATAGC AGTTCAAACG 
GTAGCAGAGA TATCTGTGCA AACTGAACCA GTTGGAACCA TAAGAACACC CTCCATACGG 
GCACGAGTGG ATGCCAAGGT AGAAATAATT AAACACATTT CAGCACCTGA AAAGACTTAC 
AAAGGGGGCA GTTTAGGATG TCAAACAGAA GCAGATTCAG ACACACAAAG TCCTCAATAT 
CTGAGTGCCA CATCTCCACC CAAAGACAAG AAACGCCCAA CACCTTTAGA GATTGGTTAT 
TCATCTCACC TCCGGGCAGA TTCCACAGTA CAGCTGGCTC CTTCCCCACC CAAATCCCCC 
AAAGTCCTTT ACTCACCCAT CTCACCACTT TCACCAGGCA AAGCCTTAGA ATCAGCCTTT 
GTACCTTATG AAAAACCCCT CCCTGATGAT ATAAGTCCAC AGAAAGTACT GCATCCAGAT 
ATGGCTAAAG TTCCCCCAGC AAGTCCTAAG ACAGCCAAGA TGATGCAGCG TTCTATGTCT 
GACCCCAAGC CTCTGAGTCC AACAGCAGAC GAAAGTTCCA GGGCTCCTTT TCAGTATACC 
GAGGGCTATA CGACTAAAGG TTCTCAAACC ATGACATCCT CTGGAGCCCA GAAAAAAGTT 
AAAAGAACTC TGCCAAATCC ACCTCCTGAG GAGATTTCCA CAGGAACTCA ATCCACATTC 
AGCACAATGG GCACAGTTTC CAGGAGAAGG ATCTGCAGAA CCAACACAAT GGCACGAGCC 
AAGATTCTCC AGGACATAGA CAGAGAGCTT GATCTTGTGG AAAGGGAGTC TGCAAAACTT 
CGAAAGAAAC AAGCAGAGCT TGATGAAGAA GAAAAGGAGA TTGATGCTAA GCTACGATAC 
CTGGAAATGG GAATTAACAG GAGGAAAGAG GCCCTATTAA AGGAGAGAGA AAAGAGAGAA 
CGAGCCTACC TCCAGGGAGT AGCTGAGGAT CGTGATTACA TGTCTGACAG TGAAGTGAGT 
AGCACAAGAC CAACCCGAAT AGAAAGTCAG CATGGCATTG AGCGACCAAG AACTGCTCCC 
CAAACTGAAT TCAGCCAGTT TATACCACCA CAAACCCAAA CAGAATCTCA ACTAGTTCCT 
CCGACAAGTC CTTACACACA ATACCAGTAC TCTTCCCCTG CTCTTCCTAC CCAAGCACCC 
ACCTCATACA CTCAACAGTC TCATTTTGAG CAACAAACTT TGTACCATCA GCAAGTTTCA 
CCTTATCAGA CTCAGCCAAC ATTCCAAGCT GTGGCAACAA TGTCCTTCAC ACCTCAAGTT 
CAACCTACAC CAACCCCACA GCCTTCTTAT CAGTTACCTT CACAGATGAT GGTGATACAA 
CAGAAGCCAC GGCAAACTAC ATTATATTTG GAGCCCAAGA TAACCTCAAA CTATGAAGTG 
ATTCGCAACC AACCCCTTAT GATAGCACCT GTTTCTACGG ATAACACATT TGCTGTTTCC 
CATCTTGGTA GTAAGTACAA TAGTTTAGAC TTGAGAATAG GTTTGGAGGA AAGAAGTAGC 
ATGGCAAGCA GTCCAATATC AAGCATATCT GCAGATTCTT TCTATGCAGA TATTGATCAC 
CATACTCCAC GAAATTATGT CCTAATTGAC GACATTGGAG AGATCACCAA AGGAACAGCG 
GCATTAAGCA CCGCATTTAG CCTTCATGAA AAGGATCTGT CAAAAACAGA CCGTCTCCTT 
CGAACCACTG AGACACGCCG GTCTCAAGAA GTGACAGATT TCCTAGCACC TTTACAGTCT 
TCCTCTAGAT TGCATAGTTA TGTGAAGGCG GAGGAAGACC CAATGGAGGA TCCTTACGAG 
TTAAAGCTTC TGAAACATCA GATTAAACAG GAATTTCGTA GAGGGACAGA GAGCTTAGAT 
CACCTTGCTG GTCTTTCTCA TTATTACCAT GCTGATACTA GCTACAGACA TTTTCCAAAA 
TCTGAGAAGT ATAGCATCAG TAGACTCACA CTTGAAAAAC AAGCAGCAAA ACAACTGCCA 
GCAGCCATAC TTTATCAAAA GCAGTCAAAG CATAAGAAAT CACTAATTGA CCCTAAAATG 
TCAAAATTTT CACCTATTCA AGAAAGTAGA GACCTTGAAC CTGATTATTC AAGCTATATG 
ACTTCTAGCA CTTCATCTAT TGGTGGCATT TCCTCCAGGG CAAGGCTCCT TCAAGATGAC 
ATCACTTTTG GCCTCAGAAA AAATATTACA GACCAACAAA AATTTATGGG ATCTTCTCTT 
GGCACAGGAC TGGGCACATT AGGAAATACC ATACGCTCAG CTCTGCAGGA TGAAGCGGAT 
AAGCCATACA GTAGTGGCAG CAGGTCCAGA CCTTCCTCCA GACCTTCCTC TGTCTATGGG 
CTTGATTTAT CAATTAAAAG GGATTCTTCT AGCTCTTCCC TAAGACTGAA AGCTCAAGAG 
GCTGAAGCTC TAGATGTTTC CTTTAGTCAT GCATCATCCT CTGCCAGAAC TAAGCCGACC 
AGTTTGCCAA TTAGTCAAAG TAGAGGAAGA ATACCAATTG TGGCCCAGAA TTCTGAAGAA 
GAAAGCCCAC TCAGTCCTGT TGGCCAGCCA ATGGGAATGG CCAGGGCTGC AGCTGGACCC 
CTGCCACCAA TATCTGCAGA CACCAGGGAT CAGTTTGGAT CAAGCCACTC ATTGCCTGAA 
GTTCAGCAAC ACATGAGGGA AGAATCACGG ACTCGAGGCT ATGACCGTGA CATAGCATTC 
ATCATGGATG ACTTCCAACA TGCCATGTCA GACAGTGAAG CCTATCATCT GCGTCGTGAG 
GAAACAGATT GGTTTGATAA ACCCAGGGAG TCTCGTTTGG AAAATGGACA TGGTCTGGAC 
CGAAAACTGC CGGAAAGATT GGTCCACTCT AGACCACTCA GTCAACATCA AGAGCAAATT 
ATACAGATGA ACGGGAAAAC TATGCACTAC ATCTTTCCTC ACGCAAGGAT AAAAATAACA 
AGAGACTCAA AGGATCACAC AGTTTCAGGT AATGGATTAG GAATTAGAAT TGTGGGTGGT 
AAAGAAATCC CGGGACATAG TGGAGAAATT GGAGCCTATA TTGCCAAGAT TCTTCCTGGG 
GGAAGTGCGG AACAGACGGG GAAGCTTATG GAAGGGATGC AAGTATTGGA ATGGAATGGA 
ATTCCCTTGA CTTCTAAAAC ATATGAAGAA GTTCAGAGTA TCATTAGTCA GCAAAGTGGG 
GAAGCAGAAA TATGTGTAAG ACTGGACCTC AATATGCTAT CAGATTCTGA AAATTCCCAG 
CATCTGGAAC TTCATGAGCC ACCAAAAGCT GTGGATAAGG CGAAATCCCC AGGGGTTGAT 
CCTAAGCAGT TGGCAGCAGA ACTCCAGAAG GTTTCACTAC AGCAGTCACC GCTGGTTCTG 
TCATCAGTTG TTGAAAAAGG ATCTCATGTT CATTCAGGTC CTACATCAGC AGGATCCAGT 
TCCGTTCCCA GCCCTGGGCA ACCAGGGTCC CCCTCAGTGA GCAAAAAGAA GCACGGCAGC 
AGCAAGCCTA CCGATGGAAC AAAGGTTGTC TCTCATCCAA TTACAGGAGA AATTCAGCTT 
CAAATTAACT ATGATCTTGG AAATCTCATA ATACATATTC TCCAAGCAAG AAATCTTGTT 
CCTCGAGACA ACAATGGTTA TTCTGACCCT TTTGTGAAAG TGTACCTTCT TCCAGGGAGA 
GGTCAAGTCA TGGTTGTCCA GAATGCAAGT GCTGAGTACA AGAGAAGGAC TAAACATGTC 
CAGAAAAGTC TTAATCCTGA GTGGAATCAA ACAGTAATTT ATAAAAGTAT TTCCATGGAA 
CAGCTCAAGA AGAAAACACT GGAGGTGACA GTTTGGGATT ATGATAGATT TTCATCCAAC 
GACTTCCTTG GGGAGGTATT GATTGATTTA TCTAGCACAT CTCACCTCGA TAACACTCCA 
AGGTGGTATC CTCTCAAAGA ACAGACTGAA AGCATTGATC ATGGCAAGTC TCATTCCAGT 
CAGAGCAGCC AGCAGTCCCC AAAGCCATCT GTTATCAAAA GCAGAAGCCA TGGTATCTTC 
CCTGACCCAT CAAAGGACAT GCAGGTTCCC ACCATTGAGA AATCCCATAG TAGTCCTGGT 
AGCTCAAAAT CATCATCAGA AGGCCATCTC CGTTCTCATG GACCATCTCG CAGTCAAAGC 
AAAACCAGCG TCACTCAGAC CCACCTGGAA GATGCAGGGG CTGCCATAGC TGCTGCCGAA 
GCTGCCGTGC AACAACTCCG CATTCAACCA AGTAAAAGAC GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCLO ( NM_033026.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_149015.2 Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant 1, mRNA.

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CloneID	OHu24277
Clone ID Related Accession (Same CDS sequence)	NM_033026.5 , NM_033026.6
Accession Version	NM_033026.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 15429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	protein piccolo isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093461.4, AC004903.2, AC004886.1, AC080093.6, AC004082.1 and AC004006.1. This sequence is a reference standard in the RefSeqGene project. On Nov 20, 2007 this sequence version replaced NM_033026.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA 
GCGGCTGGAG GAGGAGCTAG CGGGGCGGGG AGCCCCTCTC ACACCGCGAT CCCGGCCGGC 
ATGGAGGCGG ATTTGAGCCA GCTGAGCGAA GAGGAGAGGA GACAGATCGC CGCTGTCATG 
TCAAGGGCGC AGGGGCTGCC CAAGGGAAGC GTCCCCCCGG CCGCTGCGGA GTCGCCTTCC 
ATGCACAGGA AACAAGAGTT GGATAGTAGT CATCCTCCAA AGCAATCAGG AAGACCCCCG 
GACCCTGGGC GTCCAGCTCA ACCTGGTCTC AGTAAAAGTA GAACTACAGA CACTTTCAGG 
TCAGAGCAGA AATTGCCTGG GAGGAGTCCT TCCACTATTA GCTTGAAAGA ATCAAAGTCC 
AGAACTGATT TAAAGGAAGA GCACAAGTCT AGTATGATGC CTGGCTTCCT CTCAGAGGTT 
AACGCTTTAA GTGCTGTTTC CTCTGTTGTA AATAAATTCA ACCCTTTTGA TTTGATATCA 
GACTCTGAGG CATCCCAGGA AGAAACCACC AAGAAACAAA AAGTGGTTCA GAAGGAGCAA 
GGAAAACCTG AAGGAATCAT AAAACCTCCT TTACAACAAC AGCCACCCAA GCCGATTCCT 
AAGCAGCAAG GACCTGGTAG GGATCCGCTT CAGCAGGATG GCACTCCCAA ATCAATATCT 
TCTCAACAAC CAGAAAAAAT TAAATCACAA CCTCCAGGTA CAGGAAAGCC AATTCAGGGT 
CCTACCCAGA CTCCTCAGAC AGACCATGCA AAATTGCCAC TTCAACGAGA TGCATCCAGG 
CCTCAGACTA AACAGGCAGA CATAGTAAGG GGAGAATCAG TTAAACCCTC ACTGCCAAGC 
CCATCCAAAC CACCTATTCA GCAACCAACT CCTGGAAAAC CTCCAGCACA GCAGCCTGGA 
CATGAAAAAT CACAGCCTGG GCCTGCAAAG CCCCCAGCTC AGCCCTCAGG GCTAACAAAG 
CCATTGGCTC AACAACCAGG GACAGTGAAA CCCCCAGTCC AGCCACCAGG GACAACAAAG 
CCTCCAGCTC AGCCTCTTGG TCCTGCTAAG CCTCCAGCTC AGCAGACTGG GTCAGAGAAG 
CCTTCATCGG AGCAGCCTGG GCCAAAGGCT TTAGCTCAGC CTCCTGGAGT TGGAAAGACT 
CCAGCTCAAC AGCCAGGGCC AGCAAAGCCT CCAACCCAGC AGGTGGGGAC ACCAAAACCC 
CTAGCTCAAC AACCTGGGCT ACAGTCTCCA GCTAAGGCAC CTGGGCCTAC AAAGACTCCA 
GTTCAGCAGC CTGGGCCAGG AAAGATTCCA GCTCAACAGG CAGGACCCGG AAAGACTTCT 
GCCCAGCAGA CTGGCCCAAC AAAGCCTCCT TCACAACTGC CTGGCCCAGC AAAGCCCCCA 
CCTCAACAGC CTGGCCCAGC AAAGCCCCCA CCTCAACAGC CTGGCTCAGC AAAGCCCCCA 
TCTCAACAGC CTGGCTCAAC AAAACCCCCA CCTCAACAGC CTGGCCCAGC AAAGCCCTCA 
CCTCAACAGC CTGGCTCAAC AAAACCCCCA TCTCAACAGC CTGGCTCAGC AAAACCCTCA 
GCTCAACAGC CTAGCCCAGC AAAGCCCTCG GCTCAGCAAT CTACAAAACC AGTAAGCCAA 
ACAGGATCTG GAAAACCTCT GCAGCCACCA ACAGTGTCTC CATCTGCAAA ACAGCCTCCT 
TCACAAGGCC TCCCTAAAAC CATCTGTCCT CTTTGCAATA CCACTGAACT TCTGTTGCAT 
GTTCCAGAAA AGGCCAATTT TAACACATGC ACTGAGTGTC AAACCACTGT CTGTAGTCTC 
TGTGGTTTTA ATCCCAATCC TCATTTAACG GAGGTAAAAG AGTGGCTCTG TTTGAACTGT 
CAAATGAAAA GAGCTCTAGG CGGGGATCTG GCTCCAGTTC CGTCATCACC CCAGCCCAAA 
CTGAAGACTG CACCTGTTAC CACTACATCA GCAGTGAGCA AATCATCCCC ACAGCCACAG 
CAGACTTCCC CAAAGAAGGA TGCTGCACCA AAACAGGATC TCTCCAAGGC ACCTGAGCCT 
AAAAAGCCAC CACCACTAGT GAAACAACCA ACCCTTCATG GCTCTCCTTC AGCCAAGGCC 
AAGCAGCCCC CTGAGGCAGA TTCTTTGTCC AAGCCAGCCC CTCCCAAAGA ACCTTCTGTC 
CCATCTGAGC AGGACAAGGC CCCTGTTGCT GATGATAAAC CAAAGCAGCC CAAGATGGTA 
AAGCCAACCA CTGACCTTGT ATCTTCATCA TCAGCAACAA CAAAACCTGA TATTCCAAGC 
TCCAAAGTAC AGTCACAAGC TGAAGAGAAA ACAACCCCTC CTCTAAAAAC AGACTCTGCC 
AAACCCTCAC AGAGTTTTCC ACCAACAGGG GAAAAAGTCA GCCCATTTGA TTCTAAAGCC 
ATACCTCGAC CTGCATCAGA TTCAAAAATT ATTTCACATC CTGGTCCCAG TTCAGAGAGC 
AAAGGTCAAA AACAAGTTGA CCCCGTACAA AAGAAGGAAG AACCCAAGAA AGCACAAACC 
AAAATGAGTC CTAAACCAGA TGCCAAGCCA ATGCCAAAAG GGTCACCAAC ACCCCCTGGC 
CCACGACCTA CCGCTGGCCA AACTGTCCCC ACACCTCAAC AGTCCCCAAA GCCTCAGGAG 
CAGTCAAGGC GTTTCAGTCT GAATCTGGGA AGTATTACTG ATGCCCCCAA ATCACAGCCT 
ACAACTCCTC AAGAGACCGT GACTGGGAAA CTCTTTGGGT TTGGAGCATC AATCTTCAGC 
CAGGCATCAA ATTTAATTTC CACTGCAGGC CAACCTGGAC CTCATTCACA AAGTGGACCA 
GGGGCCCCAA TGAAACAAGC CCCTGCCCCT TCACAGCCAC CTACTTCACA AGGGCCACCC 
AAATCCACAG GTCAAGCACC ACCAGCACCT GCAAAAAGTA TACCTGTGAA AAAGGAAACA 
AAAGCCCCAG CAGCTGAAAA ATTAGAGCCC AAAGCTGAAC AAGCTCCAAC AGTAAAAAGA 
ACAGAAACAG AAAAAAAGCC ACCACCTATT AAGGATAGCA AATCTTTAAC AGCTGAGCCT 
CAAAAGGCTG TCCTTCCCAC AAAACTGGAG AAATCGCCCA AACCAGAATC AACCTGTCCT 
CTCTGCAAAA CTGAACTCAA CATAGGTTCT AAGGATCCTC CTAACTTCAA TACTTGCACT 
GAATGCAAGA ATCAAGTGTG TAATCTCTGT GGATTTAACC CTACACCACA TTTGACTGAG 
ATTCAAGAAT GGCTTTGTTT AAATTGCCAA ACCCAGAGAG CAATATCAGG ACAGCTTGGA 
GACATACGCA AAATGCCACC TGCACCATCA GGACCCAAAG CATCTCCTAT GCCTGTTCCT 
ACAGAATCAT CATCTCAGAA AACAGCAGTG CCTCCCCAAG TAAAATTAGT GAAAAAGCAA 
GAACAAGAAG TAAAAACGGA AGCTGAAAAA GTCATTCTGG AAAAAGTAAA GGAAACACTA 
TCAATGGAAA AAATTCCTCC TATGGTAACC ACAGATCAAA AACAAGAAGA GAGTAAACTA 
GAGAAAGACA AAGCTTCAGC TCTTCAAGAA AAAAAGCCAC TCCCTGAAGA AAAAAAACTA 
ATCCCTGAAG AAGAAAAGAT ACGTTCTGAA GAAAAAAAGC CACTCCTAGA AGAAAAAAAG 
CCAACCCCTG AAGACAAAAA GCTACTCCCA GAGGCAAAAA CATCAGCCCC AGAAGAACAG 
AAACATGACT TACTTAAATC TCAAGTACAA ATTGCTGAAG AAAAGCTTGA AGGCAGAGTG 
GCTCCAAAGA CAGTGCAAGA AGGGAAACAA CCACAGACCA AGATGGAAGG TTTACCATCT 
GGCACACCTC AGAGTTTACC TAAAGAAGAT GATAAGACAA CCAAAACAAT AAAAGAACAG 
CCACAGCCAC CATGCACAGC AAAACCTGAT CAGGTGGAAC CTGGGAAAGA AAAAACAGAA 
AAGGAAGATG ACAAATCAGA CACCTCAAGT TCTCAGCAGC CTAAAAGCCC CCAAGGTCTG 
AGCGACACGG GATATTCTTC CGATGGAATA TCAAGCTCAC TTGGTGAAAT TCCAAGTCTT 
ATTCCAACTG ATGAAAAGGA TATTCTCAAG GGACTCAAAA AGGACTCTTT TTCACAAGAA 
AGCAGCCCTT CCAGCCCCTC AGATTTGGCT AAGTTAGAAA GTACAGTCCT ATCTATTTTG 
GAAGCTCAAG CAAGTACACT TGCTGATGAA AAGTCAGAAA AGAAAACACA ACCCCATGAA 
GTTTCTCCTG AACAGCCTAA AGACCAAGAG AAAACTCAGA GTTTATCTGA AACCTTGGAA 
ATTACTATTT CAGAAGAGGA GATCAAAGAG AGTCAAGAAG AAAGGAAAGA CACTTTTAAA 
AAAGATAGCC AACAAGATAT TCCTTCCAGC AAGGACCATA AAGAGAAGTC TGAGTTTGTT 
GATGACATAA CTACTAGAAG AGAGCCTTAT GATTCAGTTG AAGAGAGTAG TGAAAGTGAA 
AACTCACCTG TTCCACAAAG AAAACGAAGA ACTAGTGTTG GCTCATCAAG CAGTGATGAG 
TATAAACAGG AAGACAGCCA AGGATCAGGG GAAGAGGAGG ACTTCATTCG AAAACAAATC 
ATAGAAATGA GTGCTGATGA AGATGCTTCA GGTTCTGAAG ATGATGAGTT CATCAGAAAC 
CAGCTCAAAG AGATTAGTAG CAGTACTGAG AGCCAGAAGA AGGAAGAAAC AAAGGGAAAA 
GGCAAAATAA CAGCAGGGAA ACACAGACGA CTGACTCGAA AAAGTAGCAC AAGCATTGAT 
GAAGATGCAG GAAGACGTCA CTCATGGCAT GATGAAGACG ATGAAGCATT TGATGAAAGT 
CCTGAACTTA AATACAGAGA AACTAAAAGT CAGGAAAGTG AAGAACTTGT AGTTACTGGA 
GGAGGAGGGC TACGCCGATT TAAAACAATT GAGCTCAACA GTACAATAGC AGATAAATAT 
TCTGCAGAGT CATCACAGAA AAAAACAAGT TTGTATTTTG ACGAAGAGCC AGAATTGGAA 
ATGGAAAGCC TGACAGACTC ACCTGAAGAT AGGTCAAGGG GAGAGGGATC TTCGAGTCTG 
CATGCTTCCA GCTTCACTCC TGGTACATCC CCTACATCAG TATCATCACT TGATGAGGAC 
AGTGACAGTA GCCCGAGTCA CAAAAAAGGA GAGAGCAAAC AGCAACGCAA AGCTCGGCAC 
AGACCACATG GCCCTCTTTT GCCTACTATT GAAGATTCTT CAGAGGAAGA AGAATTGAGA 
GAGGAAGAAG AATTATTAAA GGAGCAAGAA AAGCAGAGGG AAATAGAACA GCAACAAAGA 
AAGAGTTCTA GTAAAAAATC AAAGAAAGAC AAAGATGAAC TTCGAGCTCA GAGAAGAAGG 
GAAAGGCCAA AGACACCACC TAGTAATCTC TCTCCCATTG AAGATGCATC TCCGACAGAA 
GAGTTACGTC AGGCTGCAGA AATGGAGGAG CTCCATAGAT CTTCTTGTTC TGAATATTCA 
CCTAGCATAG AGTCAGACCC AGAAGGTTTT GAAATAAGCC CGGAAAAAAT AATAGAAGTA 
CAAAAAGTTT ATAAATTGCC CACAGCTGTT TCATTATACT CACCAACAGA TGAGCAATCT 
ATTATGCAGA AAGAAGGTAG CCAAAAGGCG TTAAAAAGTG CTGAGGAGAT GTATGAAGAA 
ATGATGCATA AAACACACAA ATACAAAGCT TTTCCAGCTG CAAATGAACG AGATGAAGTG 
TTTGAAAAAG AGCCTTTGTA TGGTGGGATG CTAATAGAGG ATTATATTTA TGAATCTTTA 
GTAGAAGACA CGTACAATGG ATCGGTAGAT GGCAGTCTGC TAACAAGGCA AGAAGAAGAA 
AATGGATTTA TGCAGCAGAA AGGAAGAGAG CAAAAGATAA GACTTTCAGA ACAGATTTAT 
GAAGATCCTA TGCAGAAAAT TACAGACCTC CAGAAAGAGT TTTATGAGTT AGAAAGCTTA 
CATTCTGTTG TGCCTCAGGA AGATATTGTT TCAAGCTCTT TTATCATCCC AGAAAGCCAT 
GAGATAGTGG ACCTGGGTAC TATGGTAACT TCTACAGAAG AAGAAAGGAA ACTACTAGAT 
GCTGATGCTG CCTATGAAGA ACTTATGAAG AGGCAACAGA TGCAATTAAC ACCTGGATCT 
AGCCCAACCC AGGCCCCCAT TGGTGAGGAT ATGACAGAGT CCACCATGGA CTTTGACAGA 
ATGCCAGATG CCTCTTTGAC ATCAAGTGTT CTCTCAGGAG CGTCTCTTAC AGATTCGACC 
AGCAGTGCAA CACTCTCTAT CCCAGATGTT AAAATAACCC AACATTTTTC AACAGAAGAA 
ATTGAGGATG AATATGTAAC CGATTATACA AGAGAAATTC AAGAGATAAT TGCCCATGAA 
TCGCTGATTT TGACCTACTC GGAGCCTTCA GAAAGTGCTA CATCTGTCCC ACCCTCTGAC 
ACACCTTCTC TCACATCATC TGTTTCTTCG GTCTGTACCA CAGATAGCTC TTCACCCATT 
ACTACCCTGG ATAGCATAAC CACAGTTTAT ACAGAGCCAG TGGACATGAT AACTAAATTT 
GAAGATTCTG AGGAAATTTC TTCATCAACT TATTTTCCAG GCAGCATTAT AGACTATCCA 
GAAGAAATAA GTGTATCTTT AGATCGGACT GCCCCACCAG ATGGTAGAGC TAGTGCTGAT 
CATATTGTTA TTTCCTTATC TGATATGGCA TCTTCTATCA TAGAATCTGT AGTACCTAAA 
CCTGAAGGGC CAGTTGCTGA CACTGTTTCT ACTGACTTAC TTATATCTGA AAAGGACCCA 
GTGAAGAAAG CCAAGAAGGA AACTGGGAAT GGAATCATTC TGGAAGTTTT GGAAGCTTAC 
AGAGATAAAA AGGAGTTGGA GGCCGAACGA ACAAAAAGTA GCTTATCCGA AACCGTGTTT 
GATCACCCAC CTTCTTCTGT AATAGCCCTT CCAATGAAAG AGCAGCTTTC AACTACATAC 
TTTACATCTG GAGAGACCTT TGGTCAGGAA AAACCTGCAT CTCAGTTACC ATCTGGCAGT 
CCTTCTGTTT CCTCTCTTCC AGCTAAACCT CGCCCATTCT TTAGAAGTTC TTCTTTGGAT 
ATATCAGCTC AACCTCCTCC CCCTCCTCCC CCTCCCCCTC CTCCTCCTCC TCCACCACCA 
CCCCCTCCTC CCCCACCACT TCCTCCACCA ACTTCACCTA AACCAACTAT TCTTCCTAAA 
AAAAAGTTAA CAGTTGCATC TCCAGTGACT ACAGCTACAC CTCTGTTTGA TGCTGTTACT 
ACTCTAGAGA CCACAGCTGT TCTGAGAAGT AATGGATTAC CTGTTACAAG AATATGTACT 
ACTGCACCTC CTCCTGTTCC TCCTAAGCCA TCTTCAATTC CATCTGGACT TGTATTTACC 
CACAGGCCTG AGCCAAGCAA ACCTCCAATC GCCCCCAAAC CAGTGATTCC TCAGCTTCCA 
ACAACTACAC AAAAACCAAC AGATATACAC CCCAAACCAA CAGGCCTATC TTTAACTTCA 
AGTATGACCT TAAATTTAGT GACTTCAGCA GATTATAAAT TGCCTTCCCC TACCTCCCCA 
CTTTCCCCAC ACTCCAACAA GTCTTCACCA AGATTTTCCA AATCCCTCAC AGAAACTTAT 
GTAGTTATTA CATTGCCATC TGAACCAGGG ACTCCAACAG ATTCTTCTGC TAGTCAAGCA 
ATTACCAGTT GGCCCTTGGG ATCACCCTCC AAAGATCTGG TTTCTGTTGA ACCTGTGTTT 
TCTGTAGTTC CTCCTGTGAC AGCTGTAGAA ATTCCAATTT CTTCAGAACA GACCTTCTAC 
ATCTCTGGAG CTTTACAGAC ATTTTCTGCT ACCCCTGTCA CAGCACCCTC TTCATTTCAA 
GCAGCTCCCA CATCAGTTAC ACAGTTTCTC ACTACTGAAG TTTCCAAGAC TGAGGTTTCA 
GCAACCAGAA GTACAGCTCC TAGTGTTGGT CTCAGCAGCA TTTCCATAAC AATTCCTCCA 
GAGCCTCTTG CTCTAGATAA CATACATTTA GAGAAGCCTC AGTATAAAGA AGATGGAAAA 
TTGCAACTTG TTGGTGATGT AATTGATTTG CGTACAGTAC CAAAGGTAGA AGTTAAAACA 
ACTGATAAAT GTATTGATCT TTCTGCTTCT ACAATGGATG TGAAAAGGCA GATCACAGCA 
AATGAAGTTT ATGGGAAACA AATTAGTGCT GTCCAACCCT CTATTATAAA TCTTAGTGTG 
ACATCATCAA TAGTGACTCC TGTATCTCTG GCCACTGAGA CAGTGACCTT TGTCACATGC 
ACAGCTAGTG CAAGTTACAC TACAGGCACA GAAAGCCTAG TGGGTGCAGA ACATGCAATG 
ACAACACCAC TCCAACTTAC AACATCAAAG CATGCTGAGC CCCCATACAG GATACCAAGT 
GACCAGGTCT TTCCTATAGC TAGGGAAGAA GCACCAATAA ACTTATCTCT AGGTACTCCA 
GCACATGCAG TGACATTGGC TATTACAAAA CCTGTCACTG TGCCTCCTGT TGGTGTCACA 
AATGGATGGA CTGATAGCAC CGTATCCCAG GGAATCACTG ATGGGGAAGT AGTGGATCTC 
AGTACAACCA AGTCTCACAG AACAGTCGTA ACAATGGATG AGTCTACTTC AAGTGTGATG 
ACCAAAATAA TAGAAGATGA AAAACCCGTT GATTTAACCG CAGGGAGAAG AGCTGTGTGC 
TGTGATGTGG TTTATAAATT ACCATTTGGA AGGAGCTGCA CAGCACAGCA GCCTGCAACT 
ACTCTTCCTG AGGATCGTTT TGGTTATAGG GATGACCACT ATCAGTATGA TCGATCAGGG 
CCATATGGTT ATAGAGGGAT TGGGGGAATG AAGCCTTCCA TGTCTGACAC AAATTTAGCA 
GAAGCTGGAC ATTTTTTCTA TAAAAGTAAG AATGCTTTTG ATTATTCTGA AGGAACTGAC 
ACAGCAGTAG ATCTGACTTC AGGGAGAGTT ACTACAGGTG AGGTAATGGA TTATTCAAGC 
AAGACTACAG GTCCATATCC AGAAACACGA CAAGTCATTT CAGGAGCTGG GATTAGTACC 
CCACAGTATT CCACAGCAAG AATGACACCA CCACCAGGAC CCCAGTATTG TGTGGGGAGT 
GTTTTGAGGT CATCTAATGG TGTTGTCTAT TCTTCAGTAG CAACTCCAAC ACCCTCTACA 
TTTGCTATCA CCACACAACC TGGCTCCATT TTCAGCACCA CAGTGAGGGA TTTGTCTGGT 
ATTCATACGG CTGATGCAGT GACTTCATTA CCTGCCATGC ACCATAGCCA GCCAATGCCT 
AGATCATATT TTATAACAAC AGGTGCATCT GAAACGGACA TTGCAGTAAC TGGTATTGAT 
ATCAGTGCCA GTTTGCAAAC TATTACTATG GAGTCTCTTA CTGCTGAGAC GATAGACTCT 
GTTCCCACTT TAACCACAGC ATCCGAAGTG TTTCCTGAAG TGGTGGGAGA TGAAAGTGCT 
CTTTTAATTG TCCCTGAAGA AGATAAACAA CAGCAGCAGC TAGACTTGGA GCGTGAGCTC 
CTGGAACTGG AGAAAATTAA GCAACAGCGC TTTGCTGAGG AATTGGAGTG GGAACGTCAG 
GAAATTCAAA GGTTCCGAGA ACAAGAAAAG ATCATGGTTC AGAAAAAGTT GGAGGAGCTG 
CAGTCTATGA AGCAACACCT TCTCTTTCAG CAAGAAGAAG AGCGGCAAGC CCAGTTCATG 
ATGAGGCAGG AGACGTTAGC TCAGCAACAG TTACAGCTTG AGCAGATCCA ACAGCTGCAA 
CAACAGCTTC ACCAGCAGCT GGAGGAGCAA AAGATTCGGC AGATCTACCA GTATAACTAT 
GACCCTTCTG GAACTGCTTC TCCACAAACC ACTACAGAGC AGGCAATTTT GGAAGGTCAG 
TATGCTGCTC TGGAAGGCAG TCAATTTTGG GCAACTGAAG ATGCAACCAC CACAGCTTCA 
GCTGTTGTGG CAATTGAAAT ACCACAAAGC CAAGGATGGT ACACCGTTCA GTCTGATGGT 
GTTACTCAGT ACATTGCCCC ACCTGGTATC CTGAGCACTG TTTCAGAAAT ACCTCTAACA 
GATGTTGTTG TGAAAGAGGA AAAACAACCC AAAAAGAGAA GTTCTGGAGC TAAAGTCCGA 
GGACAGTATG ATGACATGGG AGAAAATATG ACAGATGATC CCCGAAGTTT TAAAAAGATA 
GTGGACAGTG GTGTACAAAC GGATGACGAA GATGCCACAG ATCGGAGCTA TGTGAGTAGG 
AGAAGGAGAA CTAAAAAGAG TGTGGATACA AGCGTCCAAA CTGATGATGA AGATCAGGAT 
GAGTGGGATA TGCCTACTAG ATCAAGGAGG AAAGCTCGTG TAGGGAAATA TGGTGACAGC 
ATGACAGAGG CTGACAAGAC CAAACCCCTT TCCAAAGTCT CCAGCATAGC AGTTCAAACG 
GTAGCAGAGA TATCTGTGCA AACTGAACCA GTTGGAACCA TAAGAACACC CTCCATACGG 
GCACGAGTGG ATGCCAAGGT AGAAATAATT AAACACATTT CAGCACCTGA AAAGACTTAC 
AAAGGGGGCA GTTTAGGATG TCAAACAGAA GCAGATTCAG ACACACAAAG TCCTCAATAT 
CTGAGTGCCA CATCTCCACC CAAAGACAAG AAACGCCCAA CACCTTTAGA GATTGGTTAT 
TCATCTCACC TCCGGGCAGA TTCCACAGTA CAGCTGGCTC CTTCCCCACC CAAATCCCCC 
AAAGTCCTTT ACTCACCCAT CTCACCACTT TCACCAGGCA AAGCCTTAGA ATCAGCCTTT 
GTACCTTATG AAAAACCCCT CCCTGATGAT ATAAGTCCAC AGAAAGTACT GCATCCAGAT 
ATGGCTAAAG TTCCCCCAGC AAGTCCTAAG ACAGCCAAGA TGATGCAGCG TTCTATGTCT 
GACCCCAAGC CTCTGAGTCC AACAGCAGAC GAAAGTTCCA GGGCTCCTTT TCAGTATACC 
GAGGGCTATA CGACTAAAGG TTCTCAAACC ATGACATCCT CTGGAGCCCA GAAAAAAGTT 
AAAAGAACTC TGCCAAATCC ACCTCCTGAG GAGATTTCCA CAGGAACTCA ATCCACATTC 
AGCACAATGG GCACAGTTTC CAGGAGAAGG ATCTGCAGAA CCAACACAAT GGCACGAGCC 
AAGATTCTCC AGGACATAGA CAGAGAGCTT GATCTTGTGG AAAGGGAGTC TGCAAAACTT 
CGAAAGAAAC AAGCAGAGCT TGATGAAGAA GAAAAGGAGA TTGATGCTAA GCTACGATAC 
CTGGAAATGG GAATTAACAG GAGGAAAGAG GCCCTATTAA AGGAGAGAGA AAAGAGAGAA 
CGAGCCTACC TCCAGGGAGT AGCTGAGGAT CGTGATTACA TGTCTGACAG TGAAGTGAGT 
AGCACAAGAC CAACCCGAAT AGAAAGTCAG CATGGCATTG AGCGACCAAG AACTGCTCCC 
CAAACTGAAT TCAGCCAGTT TATACCACCA CAAACCCAAA CAGAATCTCA ACTAGTTCCT 
CCGACAAGTC CTTACACACA ATACCAGTAC TCTTCCCCTG CTCTTCCTAC CCAAGCACCC 
ACCTCATACA CTCAACAGTC TCATTTTGAG CAACAAACTT TGTACCATCA GCAAGTTTCA 
CCTTATCAGA CTCAGCCAAC ATTCCAAGCT GTGGCAACAA TGTCCTTCAC ACCTCAAGTT 
CAACCTACAC CAACCCCACA GCCTTCTTAT CAGTTACCTT CACAGATGAT GGTGATACAA 
CAGAAGCCAC GGCAAACTAC ATTATATTTG GAGCCCAAGA TAACCTCAAA CTATGAAGTG 
ATTCGCAACC AACCCCTTAT GATAGCACCT GTTTCTACGG ATAACACATT TGCTGTTTCC 
CATCTTGGTA GTAAGTACAA TAGTTTAGAC TTGAGAATAG GTTTGGAGGA AAGAAGTAGC 
ATGGCAAGCA GTCCAATATC AAGCATATCT GCAGATTCTT TCTATGCAGA TATTGATCAC 
CATACTCCAC GAAATTATGT CCTAATTGAC GACATTGGAG AGATCACCAA AGGAACAGCG 
GCATTAAGCA CCGCATTTAG CCTTCATGAA AAGGATCTGT CAAAAACAGA CCGTCTCCTT 
CGAACCACTG AGACACGCCG GTCTCAAGAA GTGACAGATT TCCTAGCACC TTTACAGTCT 
TCCTCTAGAT TGCATAGTTA TGTGAAGGCG GAGGAAGACC CAATGGAGGA TCCTTACGAG 
TTAAAGCTTC TGAAACATCA GATTAAACAG GAATTTCGTA GAGGGACAGA GAGCTTAGAT 
CACCTTGCTG GTCTTTCTCA TTATTACCAT GCTGATACTA GCTACAGACA TTTTCCAAAA 
TCTGAGAAGT ATAGCATCAG TAGACTCACA CTTGAAAAAC AAGCAGCAAA ACAACTGCCA 
GCAGCCATAC TTTATCAAAA GCAGTCAAAG CATAAGAAAT CACTAATTGA CCCTAAAATG 
TCAAAATTTT CACCTATTCA AGAAAGTAGA GACCTTGAAC CTGATTATTC AAGCTATATG 
ACTTCTAGCA CTTCATCTAT TGGTGGCATT TCCTCCAGGG CAAGGCTCCT TCAAGATGAC 
ATCACTTTTG GCCTCAGAAA AAATATTACA GACCAACAAA AATTTATGGG ATCTTCTCTT 
GGCACAGGAC TGGGCACATT AGGAAATACC ATACGCTCAG CTCTGCAGGA TGAAGCGGAT 
AAGCCATACA GTAGTGGCAG CAGGTCCAGA CCTTCCTCCA GACCTTCCTC TGTCTATGGG 
CTTGATTTAT CAATTAAAAG GGATTCTTCT AGCTCTTCCC TAAGACTGAA AGCTCAAGAG 
GCTGAAGCTC TAGATGTTTC CTTTAGTCAT GCATCATCCT CTGCCAGAAC TAAGCCGACC 
AGTTTGCCAA TTAGTCAAAG TAGAGGAAGA ATACCAATTG TGGCCCAGAA TTCTGAAGAA 
GAAAGCCCAC TCAGTCCTGT TGGCCAGCCA ATGGGAATGG CCAGGGCTGC AGCTGGACCC 
CTGCCACCAA TATCTGCAGA CACCAGGGAT CAGTTTGGAT CAAGCCACTC ATTGCCTGAA 
GTTCAGCAAC ACATGAGGGA AGAATCACGG ACTCGAGGCT ATGACCGTGA CATAGCATTC 
ATCATGGATG ACTTCCAACA TGCCATGTCA GACAGTGAAG CCTATCATCT GCGTCGTGAG 
GAAACAGATT GGTTTGATAA ACCCAGGGAG TCTCGTTTGG AAAATGGACA TGGTCTGGAC 
CGAAAACTGC CGGAAAGATT GGTCCACTCT AGACCACTCA GTCAACATCA AGAGCAAATT 
ATACAGATGA ACGGGAAAAC TATGCACTAC ATCTTTCCTC ACGCAAGGAT AAAAATAACA 
AGAGACTCAA AGGATCACAC AGTTTCAGGT AATGGATTAG GAATTAGAAT TGTGGGTGGT 
AAAGAAATCC CGGGACATAG TGGAGAAATT GGAGCCTATA TTGCCAAGAT TCTTCCTGGG 
GGAAGTGCGG AACAGACGGG GAAGCTTATG GAAGGGATGC AAGTATTGGA ATGGAATGGA 
ATTCCCTTGA CTTCTAAAAC ATATGAAGAA GTTCAGAGTA TCATTAGTCA GCAAAGTGGG 
GAAGCAGAAA TATGTGTAAG ACTGGACCTC AATATGCTAT CAGATTCTGA AAATTCCCAG 
CATCTGGAAC TTCATGAGCC ACCAAAAGCT GTGGATAAGG CGAAATCCCC AGGGGTTGAT 
CCTAAGCAGT TGGCAGCAGA ACTCCAGAAG GTTTCACTAC AGCAGTCACC GCTGGTTCTG 
TCATCAGTTG TTGAAAAAGG ATCTCATGTT CATTCAGGTC CTACATCAGC AGGATCCAGT 
TCCGTTCCCA GCCCTGGGCA ACCAGGGTCC CCCTCAGTGA GCAAAAAGAA GCACGGCAGC 
AGCAAGCCTA CCGATGGAAC AAAGGTTGTC TCTCATCCAA TTACAGGAGA AATTCAGCTT 
CAAATTAACT ATGATCTTGG AAATCTCATA ATACATATTC TCCAAGCAAG AAATCTTGTT 
CCTCGAGACA ACAATGGTTA TTCTGACCCT TTTGTGAAAG TGTACCTTCT TCCAGGGAGA 
GGTCAAGTCA TGGTTGTCCA GAATGCAAGT GCTGAGTACA AGAGAAGGAC TAAACATGTC 
CAGAAAAGTC TTAATCCTGA GTGGAATCAA ACAGTAATTT ATAAAAGTAT TTCCATGGAA 
CAGCTCAAGA AGAAAACACT GGAGGTGACA GTTTGGGATT ATGATAGATT TTCATCCAAC 
GACTTCCTTG GGGAGGTATT GATTGATTTA TCTAGCACAT CTCACCTCGA TAACACTCCA 
AGGTGGTATC CTCTCAAAGA ACAGACTGAA AGCATTGATC ATGGCAAGTC TCATTCCAGT 
CAGAGCAGCC AGCAGTCCCC AAAGCCATCT GTTATCAAAA GCAGAAGCCA TGGTATCTTC 
CCTGACCCAT CAAAGGACAT GCAGGTTCCC ACCATTGAGA AATCCCATAG TAGTCCTGGT 
AGCTCAAAAT CATCATCAGA AGGCCATCTC CGTTCTCATG GACCATCTCG CAGTCAAAGC 
AAAACCAGCG TCACTCAGAC CCACCTGGAA GATGCAGGGG CTGCCATAGC TGCTGCCGAA 
GCTGCCGTGC AACAACTCCG CATTCAACCA ACAAAGCCTC CCAATCACCG GCCTGCAGAA 
AGCTCTGTGT CCACCGGCTC CTCGGGCAGC AGCTTTGGCA GTGGGTATAG CGTGGACAGT 
GAAGGAAGCA GCAGCACTGC AGGGGAGACT AATCTATTTC CTATTCCGAG GATAGGGAAG 
ATGGGACAGA ATGGACAAGA GCCTGTAAAA CAGCCAGGGG TAGGAGTAGG ACTAGCAGAC 
ACTGAAGCTA AAACTCAGGT AATGGGAGAA ATCAAGATTG CATTGAAGAA GGAAATGAAG 
ACAGATGGTG AACAACTAAT AGTTGAAATT CTCCAATGCA GAAATATTAC ATACAAATTT 
AAGTCTCCTG ATCATCTACC AGATTTATAT GTGAAAATAT ATGTGATGAA TATTTCTACC 
CAAAAAAAGG TGATCAAGAA AAAAACAAGA GTATGCAGAC ATGATCGAGA GCCTTCGTTT 
AATGAAACTT TTCGATTCAG TCTAAGTCCT GCAGGACATT CTCTTCAGAT TTTACTTTTC 
TCCAATGGAG GGAAGTTTAT GAAAAAGACC TTGATTGGTG AAGCCTGTAT CTGGCTTGAC 
AAAGTGGATC TCAGAAAAAG AATAGTCAAC TGGCACAAAC TTTTGGTCAG TCCTACTCAA 
ACGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149015.2
CDS	290..15718
Translation	MGNEASLEGEGLPEGLAAAAAAGGGASGAGSPSHTAIPAGMEADLSQLSEEERRQIAAVMSRAQGLPKGSVPPAAAESPSMHRKQELDSSHPPKQSGRPPDPGRPAQPGLSKSRTTDTFRSEQKLPGRSPSTISLKESKSRTDLKEEHKSSMMPGFLSEVNALSAVSSVVNKFNPFDLISDSEASQEETTKKQKVVQKEQGKPEGIIKPPLQQQPPKPIPKQQGPGRDPLQQDGTPKSISSQQPEKIKSQPPGTGKPIQGPTQTPQTDHAKLPLQRDASRPQTKQADIVRGESVKPSLPSPSKPPIQQPTPGKPPAQQPGHEKSQPGPAKPPAQPSGLTKPLAQQPGTVKPPVQPPGTTKPPAQPLGPAKPPAQQTGSEKPSSEQPGPKALAQPPGVGKTPAQQPGPAKPPTQQVGTPKPLAQQPGLQSPAKAPGPTKTPVQQPGPGKIPAQQAGPGKTSAQQTGPTKPPSQLPGPAKPPPQQPGPAKPPPQQPGSAKPPSQQPGSTKPPPQQPGPAKPSPQQPGSTKPPSQQPGSAKPSAQQPSPAKPSAQQSTKPVSQTGSGKPLQPPTVSPSAKQPPSQGLPKTICPLCNTTELLLHVPEKANFNTCTECQTTVCSLCGFNPNPHLTEVKEWLCLNCQMKRALGGDLAPVPSSPQPKLKTAPVTTTSAVSKSSPQPQQTSPKKDAAPKQDLSKAPEPKKPPPLVKQPTLHGSPSAKAKQPPEADSLSKPAPPKEPSVPSEQDKAPVADDKPKQPKMVKPTTDLVSSSSATTKPDIPSSKVQSQAEEKTTPPLKTDSAKPSQSFPPTGEKVSPFDSKAIPRPASDSKIISHPGPSSESKGQKQVDPVQKKEEPKKAQTKMSPKPDAKPMPKGSPTPPGPRPTAGQTVPTPQQSPKPQEQSRRFSLNLGSITDAPKSQPTTPQETVTGKLFGFGASIFSQASNLISTAGQPGPHSQSGPGAPMKQAPAPSQPPTSQGPPKSTGQAPPAPAKSIPVKKETKAPAAEKLEPKAEQAPTVKRTETEKKPPPIKDSKSLTAEPQKAVLPTKLEKSPKPESTCPLCKTELNIGSKDPPNFNTCTECKNQVCNLCGFNPTPHLTEIQEWLCLNCQTQRAISGQLGDIRKMPPAPSGPKASPMPVPTESSSQKTAVPPQVKLVKKQEQEVKTEAEKVILEKVKETLSMEKIPPMVTTDQKQEESKLEKDKASALQEKKPLPEEKKLIPEEEKIRSEEKKPLLEEKKPTPEDKKLLPEAKTSAPEEQKHDLLKSQVQIAEEKLEGRVAPKTVQEGKQPQTKMEGLPSGTPQSLPKEDDKTTKTIKEQPQPPCTAKPDQVEPGKEKTEKEDDKSDTSSSQQPKSPQGLSDTGYSSDGISSSLGEIPSLIPTDEKDILKGLKKDSFSQESSPSSPSDLAKLESTVLSILEAQASTLADEKSEKKTQPHEVSPEQPKDQEKTQSLSETLEITISEEEIKESQEERKDTFKKDSQQDIPSSKDHKEKSEFVDDITTRREPYDSVEESSESENSPVPQRKRRTSVGSSSSDEYKQEDSQGSGEEEDFIRKQIIEMSADEDASGSEDDEFIRNQLKEISSSTESQKKEETKGKGKITAGKHRRLTRKSSTSIDEDAGRRHSWHDEDDEAFDESPELKYRETKSQESEELVVTGGGGLRRFKTIELNSTIADKYSAESSQKKTSLYFDEEPELEMESLTDSPEDRSRGEGSSSLHASSFTPGTSPTSVSSLDEDSDSSPSHKKGESKQQRKARHRPHGPLLPTIEDSSEEEELREEEELLKEQEKQREIEQQQRKSSSKKSKKDKDELRAQRRRERPKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSPSIESDPEGFEISPEKIIEVQKVYKLPTAVSLYSPTDEQSIMQKEGSQKALKSAEEMYEEMMHKTHKYKAFPAANERDEVFEKEPLYGGMLIEDYIYESLVEDTYNGSVDGSLLTRQEEENGFMQQKGREQKIRLSEQIYEDPMQKITDLQKEFYELESLHSVVPQEDIVSSSFIIPESHEIVDLGTMVTSTEEERKLLDADAAYEELMKRQQMQLTPGSSPTQAPIGEDMTESTMDFDRMPDASLTSSVLSGASLTDSTSSATLSIPDVKITQHFSTEEIEDEYVTDYTREIQEIIAHESLILTYSEPSESATSVPPSDTPSLTSSVSSVCTTDSSSPITTLDSITTVYTEPVDMITKFEDSEEISSSTYFPGSIIDYPEEISVSLDRTAPPDGRASADHIVISLSDMASSIIESVVPKPEGPVADTVSTDLLISEKDPVKKAKKETGNGIILEVLEAYRDKKELEAERTKSSLSETVFDHPPSSVIALPMKEQLSTTYFTSGETFGQEKPASQLPSGSPSVSSLPAKPRPFFRSSSLDISAQPPPPPPPPPPPPPPPPPPPPPPLPPPTSPKPTILPKKKLTVASPVTTATPLFDAVTTLETTAVLRSNGLPVTRICTTAPPPVPPKPSSIPSGLVFTHRPEPSKPPIAPKPVIPQLPTTTQKPTDIHPKPTGLSLTSSMTLNLVTSADYKLPSPTSPLSPHSNKSSPRFSKSLTETYVVITLPSEPGTPTDSSASQAITSWPLGSPSKDLVSVEPVFSVVPPVTAVEIPISSEQTFYISGALQTFSATPVTAPSSFQAAPTSVTQFLTTEVSKTEVSATRSTAPSVGLSSISITIPPEPLALDNIHLEKPQYKEDGKLQLVGDVIDLRTVPKVEVKTTDKCIDLSASTMDVKRQITANEVYGKQISAVQPSIINLSVTSSIVTPVSLATETVTFVTCTASASYTTGTESLVGAEHAMTTPLQLTTSKHAEPPYRIPSDQVFPIAREEAPINLSLGTPAHAVTLAITKPVTVPPVGVTNGWTDSTVSQGITDGEVVDLSTTKSHRTVVTMDESTSSVMTKIIEDEKPVDLTAGRRAVCCDVVYKLPFGRSCTAQQPATTLPEDRFGYRDDHYQYDRSGPYGYRGIGGMKPSMSDTNLAEAGHFFYKSKNAFDYSEGTDTAVDLTSGRVTTGEVMDYSSKTTGPYPETRQVISGAGISTPQYSTARMTPPPGPQYCVGSVLRSSNGVVYSSVATPTPSTFAITTQPGSIFSTTVRDLSGIHTADAVTSLPAMHHSQPMPRSYFITTGASETDIAVTGIDISASLQTITMESLTAETIDSVPTLTTASEVFPEVVGDESALLIVPEEDKQQQQLDLERELLELEKIKQQRFAEELEWERQEIQRFREQEKIMVQKKLEELQSMKQHLLFQQEEERQAQFMMRQETLAQQQLQLEQIQQLQQQLHQQLEEQKIRQIYQYNYDPSGTASPQTTTEQAILEGQYAALEGSQFWATEDATTTASAVVAIEIPQSQGWYTVQSDGVTQYIAPPGILSTVSEIPLTDVVVKEEKQPKKRSSGAKVRGQYDDMGENMTDDPRSFKKIVDSGVQTDDEDATDRSYVSRRRRTKKSVDTSVQTDDEDQDEWDMPTRSRRKARVGKYGDSMTEADKTKPLSKVSSIAVQTVAEISVQTEPVGTIRTPSIRARVDAKVEIIKHISAPEKTYKGGSLGCQTEADSDTQSPQYLSATSPPKDKKRPTPLEIGYSSHLRADSTVQLAPSPPKSPKVLYSPISPLSPGKALESAFVPYEKPLPDDISPQKVLHPDMAKVPPASPKTAKMMQRSMSDPKPLSPTADESSRAPFQYTEGYTTKGSQTMTSSGAQKKVKRTLPNPPPEEISTGTQSTFSTMGTVSRRRICRTNTMARAKILQDIDRELDLVERESAKLRKKQAELDEEEKEIDAKLRYLEMGINRRKEALLKEREKRERAYLQGVAEDRDYMSDSEVSSTRPTRIESQHGIERPRTAPQTEFSQFIPPQTQTESQLVPPTSPYTQYQYSSPALPTQAPTSYTQQSHFEQQTLYHQQVSPYQTQPTFQAVATMSFTPQVQPTPTPQPSYQLPSQMMVIQQKPRQTTLYLEPKITSNYEVIRNQPLMIAPVSTDNTFAVSHLGSKYNSLDLRIGLEERSSMASSPISSISADSFYADIDHHTPRNYVLIDDIGEITKGTAALSTAFSLHEKDLSKTDRLLRTTETRRSQEVTDFLAPLQSSSRLHSYVKAEEDPMEDPYELKLLKHQIKQEFRRGTESLDHLAGLSHYYHADTSYRHFPKSEKYSISRLTLEKQAAKQLPAAILYQKQSKHKKSLIDPKMSKFSPIQESRDLEPDYSSYMTSSTSSIGGISSRARLLQDDITFGLRKNITDQQKFMGSSLGTGLGTLGNTIRSALQDEADKPYSSGSRSRPSSRPSSVYGLDLSIKRDSSSSSLRLKAQEAEALDVSFSHASSSARTKPTSLPISQSRGRIPIVAQNSEEESPLSPVGQPMGMARAAAGPLPPISADTRDQFGSSHSLPEVQQHMREESRTRGYDRDIAFIMDDFQHAMSDSEAYHLRREETDWFDKPRESRLENGHGLDRKLPERLVHSRPLSQHQEQIIQMNGKTMHYIFPHARIKITRDSKDHTVSGNGLGIRIVGGKEIPGHSGEIGAYIAKILPGGSAEQTGKLMEGMQVLEWNGIPLTSKTYEEVQSIISQQSGEAEICVRLDLNMLSDSENSQHLELHEPPKAVDKAKSPGVDPKQLAAELQKVSLQQSPLVLSSVVEKGSHVHSGPTSAGSSSVPSPGQPGSPSVSKKKHGSSKPTDGTKVVSHPITGEIQLQINYDLGNLIIHILQARNLVPRDNNGYSDPFVKVYLLPGRGQVMVVQNASAEYKRRTKHVQKSLNPEWNQTVIYKSISMEQLKKKTLEVTVWDYDRFSSNDFLGEVLIDLSSTSHLDNTPRWYPLKEQTESIDHGKSHSSQSSQQSPKPSVIKSRSHGIFPDPSKDMQVPTIEKSHSSPGSSKSSSEGHLRSHGPSRSQSKTSVTQTHLEDAGAAIAAAEAAVQQLRIQPTKPPNHRPAESSVSTGSSGSSFGSGYSVDSEGSSSTAGETNLFPIPRIGKMGQNGQEPVKQPGVGVGLADTEAKTQVMGEIKIALKKEMKTDGEQLIVEILQCRNITYKFKSPDHLPDLYVKIYVMNISTQKKVIKKKTRVCRHDREPSFNETFRFSLSPAGHSLQILLFSNGGKFMKKTLIGEACIWLDKVDLRKRIVNWHKLLVSPTQTH

Target ORF information:

RefSeq Version	NM_033026.5
Organism	Homo sapiens(human)
Definition	Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033026.5

ORF Insert Sequence:

ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA 
GCGGCTGGAG GAGGAGCTAG CGGGGCGGGG AGCCCCTCTC ACACCGCGAT CCCGGCCGGC 
ATGGAGGCGG ATTTGAGCCA GCTGAGCGAA GAGGAGAGGA GACAGATCGC CGCTGTCATG 
TCAAGGGCGC AGGGGCTGCC CAAGGGAAGC GTCCCCCCGG CCGCTGCGGA GTCGCCTTCC 
ATGCACAGGA AACAAGAGTT GGATAGTAGT CATCCTCCAA AGCAATCAGG AAGACCCCCG 
GACCCTGGGC GTCCAGCTCA ACCTGGTCTC AGTAAAAGTA GAACTACAGA CACTTTCAGG 
TCAGAGCAGA AATTGCCTGG GAGGAGTCCT TCCACTATTA GCTTGAAAGA ATCAAAGTCC 
AGAACTGATT TAAAGGAAGA GCACAAGTCT AGTATGATGC CTGGCTTCCT CTCAGAGGTT 
AACGCTTTAA GTGCTGTTTC CTCTGTTGTA AATAAATTCA ACCCTTTTGA TTTGATATCA 
GACTCTGAGG CATCCCAGGA AGAAACCACC AAGAAACAAA AAGTGGTTCA GAAGGAGCAA 
GGAAAACCTG AAGGAATCAT AAAACCTCCT TTACAACAAC AGCCACCCAA GCCGATTCCT 
AAGCAGCAAG GACCTGGTAG GGATCCGCTT CAGCAGGATG GCACTCCCAA ATCAATATCT 
TCTCAACAAC CAGAAAAAAT TAAATCACAA CCTCCAGGTA CAGGAAAGCC AATTCAGGGT 
CCTACCCAGA CTCCTCAGAC AGACCATGCA AAATTGCCAC TTCAACGAGA TGCATCCAGG 
CCTCAGACTA AACAGGCAGA CATAGTAAGG GGAGAATCAG TTAAACCCTC ACTGCCAAGC 
CCATCCAAAC CACCTATTCA GCAACCAACT CCTGGAAAAC CTCCAGCACA GCAGCCTGGA 
CATGAAAAAT CACAGCCTGG GCCTGCAAAG CCCCCAGCTC AGCCCTCAGG GCTAACAAAG 
CCATTGGCTC AACAACCAGG GACAGTGAAA CCCCCAGTCC AGCCACCAGG GACAACAAAG 
CCTCCAGCTC AGCCTCTTGG TCCTGCTAAG CCTCCAGCTC AGCAGACTGG GTCAGAGAAG 
CCTTCATCGG AGCAGCCTGG GCCAAAGGCT TTAGCTCAGC CTCCTGGAGT TGGAAAGACT 
CCAGCTCAAC AGCCAGGGCC AGCAAAGCCT CCAACCCAGC AGGTGGGGAC ACCAAAACCC 
CTAGCTCAAC AACCTGGGCT ACAGTCTCCA GCTAAGGCAC CTGGGCCTAC AAAGACTCCA 
GTTCAGCAGC CTGGGCCAGG AAAGATTCCA GCTCAACAGG CAGGACCCGG AAAGACTTCT 
GCCCAGCAGA CTGGCCCAAC AAAGCCTCCT TCACAACTGC CTGGCCCAGC AAAGCCCCCA 
CCTCAACAGC CTGGCCCAGC AAAGCCCCCA CCTCAACAGC CTGGCTCAGC AAAGCCCCCA 
TCTCAACAGC CTGGCTCAAC AAAACCCCCA CCTCAACAGC CTGGCCCAGC AAAGCCCTCA 
CCTCAACAGC CTGGCTCAAC AAAACCCCCA TCTCAACAGC CTGGCTCAGC AAAACCCTCA 
GCTCAACAGC CTAGCCCAGC AAAGCCCTCG GCTCAGCAAT CTACAAAACC AGTAAGCCAA 
ACAGGATCTG GAAAACCTCT GCAGCCACCA ACAGTGTCTC CATCTGCAAA ACAGCCTCCT 
TCACAAGGCC TCCCTAAAAC CATCTGTCCT CTTTGCAATA CCACTGAACT TCTGTTGCAT 
GTTCCAGAAA AGGCCAATTT TAACACATGC ACTGAGTGTC AAACCACTGT CTGTAGTCTC 
TGTGGTTTTA ATCCCAATCC TCATTTAACG GAGGTAAAAG AGTGGCTCTG TTTGAACTGT 
CAAATGAAAA GAGCTCTAGG CGGGGATCTG GCTCCAGTTC CGTCATCACC CCAGCCCAAA 
CTGAAGACTG CACCTGTTAC CACTACATCA GCAGTGAGCA AATCATCCCC ACAGCCACAG 
CAGACTTCCC CAAAGAAGGA TGCTGCACCA AAACAGGATC TCTCCAAGGC ACCTGAGCCT 
AAAAAGCCAC CACCACTAGT GAAACAACCA ACCCTTCATG GCTCTCCTTC AGCCAAGGCC 
AAGCAGCCCC CTGAGGCAGA TTCTTTGTCC AAGCCAGCCC CTCCCAAAGA ACCTTCTGTC 
CCATCTGAGC AGGACAAGGC CCCTGTTGCT GATGATAAAC CAAAGCAGCC CAAGATGGTA 
AAGCCAACCA CTGACCTTGT ATCTTCATCA TCAGCAACAA CAAAACCTGA TATTCCAAGC 
TCCAAAGTAC AGTCACAAGC TGAAGAGAAA ACAACCCCTC CTCTAAAAAC AGACTCTGCC 
AAACCCTCAC AGAGTTTTCC ACCAACAGGG GAAAAAGTCA GCCCATTTGA TTCTAAAGCC 
ATACCTCGAC CTGCATCAGA TTCAAAAATT ATTTCACATC CTGGTCCCAG TTCAGAGAGC 
AAAGGTCAAA AACAAGTTGA CCCCGTACAA AAGAAGGAAG AACCCAAGAA AGCACAAACC 
AAAATGAGTC CTAAACCAGA TGCCAAGCCA ATGCCAAAAG GGTCACCAAC ACCCCCTGGC 
CCACGACCTA CCGCTGGCCA AACTGTCCCC ACACCTCAAC AGTCCCCAAA GCCTCAGGAG 
CAGTCAAGGC GTTTCAGTCT GAATCTGGGA AGTATTACTG ATGCCCCCAA ATCACAGCCT 
ACAACTCCTC AAGAGACCGT GACTGGGAAA CTCTTTGGGT TTGGAGCATC AATCTTCAGC 
CAGGCATCAA ATTTAATTTC CACTGCAGGC CAACCTGGAC CTCATTCACA AAGTGGACCA 
GGGGCCCCAA TGAAACAAGC CCCTGCCCCT TCACAGCCAC CTACTTCACA AGGGCCACCC 
AAATCCACAG GTCAAGCACC ACCAGCACCT GCAAAAAGTA TACCTGTGAA AAAGGAAACA 
AAAGCCCCAG CAGCTGAAAA ATTAGAGCCC AAAGCTGAAC AAGCTCCAAC AGTAAAAAGA 
ACAGAAACAG AAAAAAAGCC ACCACCTATT AAGGATAGCA AATCTTTAAC AGCTGAGCCT 
CAAAAGGCTG TCCTTCCCAC AAAACTGGAG AAATCGCCCA AACCAGAATC AACCTGTCCT 
CTCTGCAAAA CTGAACTCAA CATAGGTTCT AAGGATCCTC CTAACTTCAA TACTTGCACT 
GAATGCAAGA ATCAAGTGTG TAATCTCTGT GGATTTAACC CTACACCACA TTTGACTGAG 
ATTCAAGAAT GGCTTTGTTT AAATTGCCAA ACCCAGAGAG CAATATCAGG ACAGCTTGGA 
GACATACGCA AAATGCCACC TGCACCATCA GGACCCAAAG CATCTCCTAT GCCTGTTCCT 
ACAGAATCAT CATCTCAGAA AACAGCAGTG CCTCCCCAAG TAAAATTAGT GAAAAAGCAA 
GAACAAGAAG TAAAAACGGA AGCTGAAAAA GTCATTCTGG AAAAAGTAAA GGAAACACTA 
TCAATGGAAA AAATTCCTCC TATGGTAACC ACAGATCAAA AACAAGAAGA GAGTAAACTA 
GAGAAAGACA AAGCTTCAGC TCTTCAAGAA AAAAAGCCAC TCCCTGAAGA AAAAAAACTA 
ATCCCTGAAG AAGAAAAGAT ACGTTCTGAA GAAAAAAAGC CACTCCTAGA AGAAAAAAAG 
CCAACCCCTG AAGACAAAAA GCTACTCCCA GAGGCAAAAA CATCAGCCCC AGAAGAACAG 
AAACATGACT TACTTAAATC TCAAGTACAA ATTGCTGAAG AAAAGCTTGA AGGCAGAGTG 
GCTCCAAAGA CAGTGCAAGA AGGGAAACAA CCACAGACCA AGATGGAAGG TTTACCATCT 
GGCACACCTC AGAGTTTACC TAAAGAAGAT GATAAGACAA CCAAAACAAT AAAAGAACAG 
CCACAGCCAC CATGCACAGC AAAACCTGAT CAGGTGGAAC CTGGGAAAGA AAAAACAGAA 
AAGGAAGATG ACAAATCAGA CACCTCAAGT TCTCAGCAGC CTAAAAGCCC CCAAGGTCTG 
AGCGACACGG GATATTCTTC CGATGGAATA TCAAGCTCAC TTGGTGAAAT TCCAAGTCTT 
ATTCCAACTG ATGAAAAGGA TATTCTCAAG GGACTCAAAA AGGACTCTTT TTCACAAGAA 
AGCAGCCCTT CCAGCCCCTC AGATTTGGCT AAGTTAGAAA GTACAGTCCT ATCTATTTTG 
GAAGCTCAAG CAAGTACACT TGCTGATGAA AAGTCAGAAA AGAAAACACA ACCCCATGAA 
GTTTCTCCTG AACAGCCTAA AGACCAAGAG AAAACTCAGA GTTTATCTGA AACCTTGGAA 
ATTACTATTT CAGAAGAGGA GATCAAAGAG AGTCAAGAAG AAAGGAAAGA CACTTTTAAA 
AAAGATAGCC AACAAGATAT TCCTTCCAGC AAGGACCATA AAGAGAAGTC TGAGTTTGTT 
GATGACATAA CTACTAGAAG AGAGCCTTAT GATTCAGTTG AAGAGAGTAG TGAAAGTGAA 
AACTCACCTG TTCCACAAAG AAAACGAAGA ACTAGTGTTG GCTCATCAAG CAGTGATGAG 
TATAAACAGG AAGACAGCCA AGGATCAGGG GAAGAGGAGG ACTTCATTCG AAAACAAATC 
ATAGAAATGA GTGCTGATGA AGATGCTTCA GGTTCTGAAG ATGATGAGTT CATCAGAAAC 
CAGCTCAAAG AGATTAGTAG CAGTACTGAG AGCCAGAAGA AGGAAGAAAC AAAGGGAAAA 
GGCAAAATAA CAGCAGGGAA ACACAGACGA CTGACTCGAA AAAGTAGCAC AAGCATTGAT 
GAAGATGCAG GAAGACGTCA CTCATGGCAT GATGAAGACG ATGAAGCATT TGATGAAAGT 
CCTGAACTTA AATACAGAGA AACTAAAAGT CAGGAAAGTG AAGAACTTGT AGTTACTGGA 
GGAGGAGGGC TACGCCGATT TAAAACAATT GAGCTCAACA GTACAATAGC AGATAAATAT 
TCTGCAGAGT CATCACAGAA AAAAACAAGT TTGTATTTTG ACGAAGAGCC AGAATTGGAA 
ATGGAAAGCC TGACAGACTC ACCTGAAGAT AGGTCAAGGG GAGAGGGATC TTCGAGTCTG 
CATGCTTCCA GCTTCACTCC TGGTACATCC CCTACATCAG TATCATCACT TGATGAGGAC 
AGTGACAGTA GCCCGAGTCA CAAAAAAGGA GAGAGCAAAC AGCAACGCAA AGCTCGGCAC 
AGACCACATG GCCCTCTTTT GCCTACTATT GAAGATTCTT CAGAGGAAGA AGAATTGAGA 
GAGGAAGAAG AATTATTAAA GGAGCAAGAA AAGCAGAGGG AAATAGAACA GCAACAAAGA 
AAGAGTTCTA GTAAAAAATC AAAGAAAGAC AAAGATGAAC TTCGAGCTCA GAGAAGAAGG 
GAAAGGCCAA AGACACCACC TAGTAATCTC TCTCCCATTG AAGATGCATC TCCGACAGAA 
GAGTTACGTC AGGCTGCAGA AATGGAGGAG CTCCATAGAT CTTCTTGTTC TGAATATTCA 
CCTAGCATAG AGTCAGACCC AGAAGGTTTT GAAATAAGCC CGGAAAAAAT AATAGAAGTA 
CAAAAAGTTT ATAAATTGCC CACAGCTGTT TCATTATACT CACCAACAGA TGAGCAATCT 
ATTATGCAGA AAGAAGGTAG CCAAAAGGCG TTAAAAAGTG CTGAGGAGAT GTATGAAGAA 
ATGATGCATA AAACACACAA ATACAAAGCT TTTCCAGCTG CAAATGAACG AGATGAAGTG 
TTTGAAAAAG AGCCTTTGTA TGGTGGGATG CTAATAGAGG ATTATATTTA TGAATCTTTA 
GTAGAAGACA CGTACAATGG ATCGGTAGAT GGCAGTCTGC TAACAAGGCA AGAAGAAGAA 
AATGGATTTA TGCAGCAGAA AGGAAGAGAG CAAAAGATAA GACTTTCAGA ACAGATTTAT 
GAAGATCCTA TGCAGAAAAT TACAGACCTC CAGAAAGAGT TTTATGAGTT AGAAAGCTTA 
CATTCTGTTG TGCCTCAGGA AGATATTGTT TCAAGCTCTT TTATCATCCC AGAAAGCCAT 
GAGATAGTGG ACCTGGGTAC TATGGTAACT TCTACAGAAG AAGAAAGGAA ACTACTAGAT 
GCTGATGCTG CCTATGAAGA ACTTATGAAG AGGCAACAGA TGCAATTAAC ACCTGGATCT 
AGCCCAACCC AGGCCCCCAT TGGTGAGGAT ATGACAGAGT CCACCATGGA CTTTGACAGA 
ATGCCAGATG CCTCTTTGAC ATCAAGTGTT CTCTCAGGAG CGTCTCTTAC AGATTCGACC 
AGCAGTGCAA CACTCTCTAT CCCAGATGTT AAAATAACCC AACATTTTTC AACAGAAGAA 
ATTGAGGATG AATATGTAAC CGATTATACA AGAGAAATTC AAGAGATAAT TGCCCATGAA 
TCGCTGATTT TGACCTACTC GGAGCCTTCA GAAAGTGCTA CATCTGTCCC ACCCTCTGAC 
ACACCTTCTC TCACATCATC TGTTTCTTCG GTCTGTACCA CAGATAGCTC TTCACCCATT 
ACTACCCTGG ATAGCATAAC CACAGTTTAT ACAGAGCCAG TGGACATGAT AACTAAATTT 
GAAGATTCTG AGGAAATTTC TTCATCAACT TATTTTCCAG GCAGCATTAT AGACTATCCA 
GAAGAAATAA GTGTATCTTT AGATCGGACT GCCCCACCAG ATGGTAGAGC TAGTGCTGAT 
CATATTGTTA TTTCCTTATC TGATATGGCA TCTTCTATCA TAGAATCTGT AGTACCTAAA 
CCTGAAGGGC CAGTTGCTGA CACTGTTTCT ACTGACTTAC TTATATCTGA AAAGGACCCA 
GTGAAGAAAG CCAAGAAGGA AACTGGGAAT GGAATCATTC TGGAAGTTTT GGAAGCTTAC 
AGAGATAAAA AGGAGTTGGA GGCCGAACGA ACAAAAAGTA GCTTATCCGA AACCGTGTTT 
GATCACCCAC CTTCTTCTGT AATAGCCCTT CCAATGAAAG AGCAGCTTTC AACTACATAC 
TTTACATCTG GAGAGACCTT TGGTCAGGAA AAACCTGCAT CTCAGTTACC ATCTGGCAGT 
CCTTCTGTTT CCTCTCTTCC AGCTAAACCT CGCCCATTCT TTAGAAGTTC TTCTTTGGAT 
ATATCAGCTC AACCTCCTCC CCCTCCTCCC CCTCCCCCTC CTCCTCCTCC TCCACCACCA 
CCCCCTCCTC CCCCACCACT TCCTCCACCA ACTTCACCTA AACCAACTAT TCTTCCTAAA 
AAAAAGTTAA CAGTTGCATC TCCAGTGACT ACAGCTACAC CTCTGTTTGA TGCTGTTACT 
ACTCTAGAGA CCACAGCTGT TCTGAGAAGT AATGGATTAC CTGTTACAAG AATATGTACT 
ACTGCACCTC CTCCTGTTCC TCCTAAGCCA TCTTCAATTC CATCTGGACT TGTATTTACC 
CACAGGCCTG AGCCAAGCAA ACCTCCAATC GCCCCCAAAC CAGTGATTCC TCAGCTTCCA 
ACAACTACAC AAAAACCAAC AGATATACAC CCCAAACCAA CAGGCCTATC TTTAACTTCA 
AGTATGACCT TAAATTTAGT GACTTCAGCA GATTATAAAT TGCCTTCCCC TACCTCCCCA 
CTTTCCCCAC ACTCCAACAA GTCTTCACCA AGATTTTCCA AATCCCTCAC AGAAACTTAT 
GTAGTTATTA CATTGCCATC TGAACCAGGG ACTCCAACAG ATTCTTCTGC TAGTCAAGCA 
ATTACCAGTT GGCCCTTGGG ATCACCCTCC AAAGATCTGG TTTCTGTTGA ACCTGTGTTT 
TCTGTAGTTC CTCCTGTGAC AGCTGTAGAA ATTCCAATTT CTTCAGAACA GACCTTCTAC 
ATCTCTGGAG CTTTACAGAC ATTTTCTGCT ACCCCTGTCA CAGCACCCTC TTCATTTCAA 
GCAGCTCCCA CATCAGTTAC ACAGTTTCTC ACTACTGAAG TTTCCAAGAC TGAGGTTTCA 
GCAACCAGAA GTACAGCTCC TAGTGTTGGT CTCAGCAGCA TTTCCATAAC AATTCCTCCA 
GAGCCTCTTG CTCTAGATAA CATACATTTA GAGAAGCCTC AGTATAAAGA AGATGGAAAA 
TTGCAACTTG TTGGTGATGT AATTGATTTG CGTACAGTAC CAAAGGTAGA AGTTAAAACA 
ACTGATAAAT GTATTGATCT TTCTGCTTCT ACAATGGATG TGAAAAGGCA GATCACAGCA 
AATGAAGTTT ATGGGAAACA AATTAGTGCT GTCCAACCCT CTATTATAAA TCTTAGTGTG 
ACATCATCAA TAGTGACTCC TGTATCTCTG GCCACTGAGA CAGTGACCTT TGTCACATGC 
ACAGCTAGTG CAAGTTACAC TACAGGCACA GAAAGCCTAG TGGGTGCAGA ACATGCAATG 
ACAACACCAC TCCAACTTAC AACATCAAAG CATGCTGAGC CCCCATACAG GATACCAAGT 
GACCAGGTCT TTCCTATAGC TAGGGAAGAA GCACCAATAA ACTTATCTCT AGGTACTCCA 
GCACATGCAG TGACATTGGC TATTACAAAA CCTGTCACTG TGCCTCCTGT TGGTGTCACA 
AATGGATGGA CTGATAGCAC CGTATCCCAG GGAATCACTG ATGGGGAAGT AGTGGATCTC 
AGTACAACCA AGTCTCACAG AACAGTCGTA ACAATGGATG AGTCTACTTC AAGTGTGATG 
ACCAAAATAA TAGAAGATGA AAAACCCGTT GATTTAACCG CAGGGAGAAG AGCTGTGTGC 
TGTGATGTGG TTTATAAATT ACCATTTGGA AGGAGCTGCA CAGCACAGCA GCCTGCAACT 
ACTCTTCCTG AGGATCGTTT TGGTTATAGG GATGACCACT ATCAGTATGA TCGATCAGGG 
CCATATGGTT ATAGAGGGAT TGGGGGAATG AAGCCTTCCA TGTCTGACAC AAATTTAGCA 
GAAGCTGGAC ATTTTTTCTA TAAAAGTAAG AATGCTTTTG ATTATTCTGA AGGAACTGAC 
ACAGCAGTAG ATCTGACTTC AGGGAGAGTT ACTACAGGTG AGGTAATGGA TTATTCAAGC 
AAGACTACAG GTCCATATCC AGAAACACGA CAAGTCATTT CAGGAGCTGG GATTAGTACC 
CCACAGTATT CCACAGCAAG AATGACACCA CCACCAGGAC CCCAGTATTG TGTGGGGAGT 
GTTTTGAGGT CATCTAATGG TGTTGTCTAT TCTTCAGTAG CAACTCCAAC ACCCTCTACA 
TTTGCTATCA CCACACAACC TGGCTCCATT TTCAGCACCA CAGTGAGGGA TTTGTCTGGT 
ATTCATACGG CTGATGCAGT GACTTCATTA CCTGCCATGC ACCATAGCCA GCCAATGCCT 
AGATCATATT TTATAACAAC AGGTGCATCT GAAACGGACA TTGCAGTAAC TGGTATTGAT 
ATCAGTGCCA GTTTGCAAAC TATTACTATG GAGTCTCTTA CTGCTGAGAC GATAGACTCT 
GTTCCCACTT TAACCACAGC ATCCGAAGTG TTTCCTGAAG TGGTGGGAGA TGAAAGTGCT 
CTTTTAATTG TCCCTGAAGA AGATAAACAA CAGCAGCAGC TAGACTTGGA GCGTGAGCTC 
CTGGAACTGG AGAAAATTAA GCAACAGCGC TTTGCTGAGG AATTGGAGTG GGAACGTCAG 
GAAATTCAAA GGTTCCGAGA ACAAGAAAAG ATCATGGTTC AGAAAAAGTT GGAGGAGCTG 
CAGTCTATGA AGCAACACCT TCTCTTTCAG CAAGAAGAAG AGCGGCAAGC CCAGTTCATG 
ATGAGGCAGG AGACGTTAGC TCAGCAACAG TTACAGCTTG AGCAGATCCA ACAGCTGCAA 
CAACAGCTTC ACCAGCAGCT GGAGGAGCAA AAGATTCGGC AGATCTACCA GTATAACTAT 
GACCCTTCTG GAACTGCTTC TCCACAAACC ACTACAGAGC AGGCAATTTT GGAAGGTCAG 
TATGCTGCTC TGGAAGGCAG TCAATTTTGG GCAACTGAAG ATGCAACCAC CACAGCTTCA 
GCTGTTGTGG CAATTGAAAT ACCACAAAGC CAAGGATGGT ACACCGTTCA GTCTGATGGT 
GTTACTCAGT ACATTGCCCC ACCTGGTATC CTGAGCACTG TTTCAGAAAT ACCTCTAACA 
GATGTTGTTG TGAAAGAGGA AAAACAACCC AAAAAGAGAA GTTCTGGAGC TAAAGTCCGA 
GGACAGTATG ATGACATGGG AGAAAATATG ACAGATGATC CCCGAAGTTT TAAAAAGATA 
GTGGACAGTG GTGTACAAAC GGATGACGAA GATGCCACAG ATCGGAGCTA TGTGAGTAGG 
AGAAGGAGAA CTAAAAAGAG TGTGGATACA AGCGTCCAAA CTGATGATGA AGATCAGGAT 
GAGTGGGATA TGCCTACTAG ATCAAGGAGG AAAGCTCGTG TAGGGAAATA TGGTGACAGC 
ATGACAGAGG CTGACAAGAC CAAACCCCTT TCCAAAGTCT CCAGCATAGC AGTTCAAACG 
GTAGCAGAGA TATCTGTGCA AACTGAACCA GTTGGAACCA TAAGAACACC CTCCATACGG 
GCACGAGTGG ATGCCAAGGT AGAAATAATT AAACACATTT CAGCACCTGA AAAGACTTAC 
AAAGGGGGCA GTTTAGGATG TCAAACAGAA GCAGATTCAG ACACACAAAG TCCTCAATAT 
CTGAGTGCCA CATCTCCACC CAAAGACAAG AAACGCCCAA CACCTTTAGA GATTGGTTAT 
TCATCTCACC TCCGGGCAGA TTCCACAGTA CAGCTGGCTC CTTCCCCACC CAAATCCCCC 
AAAGTCCTTT ACTCACCCAT CTCACCACTT TCACCAGGCA AAGCCTTAGA ATCAGCCTTT 
GTACCTTATG AAAAACCCCT CCCTGATGAT ATAAGTCCAC AGAAAGTACT GCATCCAGAT 
ATGGCTAAAG TTCCCCCAGC AAGTCCTAAG ACAGCCAAGA TGATGCAGCG TTCTATGTCT 
GACCCCAAGC CTCTGAGTCC AACAGCAGAC GAAAGTTCCA GGGCTCCTTT TCAGTATACC 
GAGGGCTATA CGACTAAAGG TTCTCAAACC ATGACATCCT CTGGAGCCCA GAAAAAAGTT 
AAAAGAACTC TGCCAAATCC ACCTCCTGAG GAGATTTCCA CAGGAACTCA ATCCACATTC 
AGCACAATGG GCACAGTTTC CAGGAGAAGG ATCTGCAGAA CCAACACAAT GGCACGAGCC 
AAGATTCTCC AGGACATAGA CAGAGAGCTT GATCTTGTGG AAAGGGAGTC TGCAAAACTT 
CGAAAGAAAC AAGCAGAGCT TGATGAAGAA GAAAAGGAGA TTGATGCTAA GCTACGATAC 
CTGGAAATGG GAATTAACAG GAGGAAAGAG GCCCTATTAA AGGAGAGAGA AAAGAGAGAA 
CGAGCCTACC TCCAGGGAGT AGCTGAGGAT CGTGATTACA TGTCTGACAG TGAAGTGAGT 
AGCACAAGAC CAACCCGAAT AGAAAGTCAG CATGGCATTG AGCGACCAAG AACTGCTCCC 
CAAACTGAAT TCAGCCAGTT TATACCACCA CAAACCCAAA CAGAATCTCA ACTAGTTCCT 
CCGACAAGTC CTTACACACA ATACCAGTAC TCTTCCCCTG CTCTTCCTAC CCAAGCACCC 
ACCTCATACA CTCAACAGTC TCATTTTGAG CAACAAACTT TGTACCATCA GCAAGTTTCA 
CCTTATCAGA CTCAGCCAAC ATTCCAAGCT GTGGCAACAA TGTCCTTCAC ACCTCAAGTT 
CAACCTACAC CAACCCCACA GCCTTCTTAT CAGTTACCTT CACAGATGAT GGTGATACAA 
CAGAAGCCAC GGCAAACTAC ATTATATTTG GAGCCCAAGA TAACCTCAAA CTATGAAGTG 
ATTCGCAACC AACCCCTTAT GATAGCACCT GTTTCTACGG ATAACACATT TGCTGTTTCC 
CATCTTGGTA GTAAGTACAA TAGTTTAGAC TTGAGAATAG GTTTGGAGGA AAGAAGTAGC 
ATGGCAAGCA GTCCAATATC AAGCATATCT GCAGATTCTT TCTATGCAGA TATTGATCAC 
CATACTCCAC GAAATTATGT CCTAATTGAC GACATTGGAG AGATCACCAA AGGAACAGCG 
GCATTAAGCA CCGCATTTAG CCTTCATGAA AAGGATCTGT CAAAAACAGA CCGTCTCCTT 
CGAACCACTG AGACACGCCG GTCTCAAGAA GTGACAGATT TCCTAGCACC TTTACAGTCT 
TCCTCTAGAT TGCATAGTTA TGTGAAGGCG GAGGAAGACC CAATGGAGGA TCCTTACGAG 
TTAAAGCTTC TGAAACATCA GATTAAACAG GAATTTCGTA GAGGGACAGA GAGCTTAGAT 
CACCTTGCTG GTCTTTCTCA TTATTACCAT GCTGATACTA GCTACAGACA TTTTCCAAAA 
TCTGAGAAGT ATAGCATCAG TAGACTCACA CTTGAAAAAC AAGCAGCAAA ACAACTGCCA 
GCAGCCATAC TTTATCAAAA GCAGTCAAAG CATAAGAAAT CACTAATTGA CCCTAAAATG 
TCAAAATTTT CACCTATTCA AGAAAGTAGA GACCTTGAAC CTGATTATTC AAGCTATATG 
ACTTCTAGCA CTTCATCTAT TGGTGGCATT TCCTCCAGGG CAAGGCTCCT TCAAGATGAC 
ATCACTTTTG GCCTCAGAAA AAATATTACA GACCAACAAA AATTTATGGG ATCTTCTCTT 
GGCACAGGAC TGGGCACATT AGGAAATACC ATACGCTCAG CTCTGCAGGA TGAAGCGGAT 
AAGCCATACA GTAGTGGCAG CAGGTCCAGA CCTTCCTCCA GACCTTCCTC TGTCTATGGG 
CTTGATTTAT CAATTAAAAG GGATTCTTCT AGCTCTTCCC TAAGACTGAA AGCTCAAGAG 
GCTGAAGCTC TAGATGTTTC CTTTAGTCAT GCATCATCCT CTGCCAGAAC TAAGCCGACC 
AGTTTGCCAA TTAGTCAAAG TAGAGGAAGA ATACCAATTG TGGCCCAGAA TTCTGAAGAA 
GAAAGCCCAC TCAGTCCTGT TGGCCAGCCA ATGGGAATGG CCAGGGCTGC AGCTGGACCC 
CTGCCACCAA TATCTGCAGA CACCAGGGAT CAGTTTGGAT CAAGCCACTC ATTGCCTGAA 
GTTCAGCAAC ACATGAGGGA AGAATCACGG ACTCGAGGCT ATGACCGTGA CATAGCATTC 
ATCATGGATG ACTTCCAACA TGCCATGTCA GACAGTGAAG CCTATCATCT GCGTCGTGAG 
GAAACAGATT GGTTTGATAA ACCCAGGGAG TCTCGTTTGG AAAATGGACA TGGTCTGGAC 
CGAAAACTGC CGGAAAGATT GGTCCACTCT AGACCACTCA GTCAACATCA AGAGCAAATT 
ATACAGATGA ACGGGAAAAC TATGCACTAC ATCTTTCCTC ACGCAAGGAT AAAAATAACA 
AGAGACTCAA AGGATCACAC AGTTTCAGGT AATGGATTAG GAATTAGAAT TGTGGGTGGT 
AAAGAAATCC CGGGACATAG TGGAGAAATT GGAGCCTATA TTGCCAAGAT TCTTCCTGGG 
GGAAGTGCGG AACAGACGGG GAAGCTTATG GAAGGGATGC AAGTATTGGA ATGGAATGGA 
ATTCCCTTGA CTTCTAAAAC ATATGAAGAA GTTCAGAGTA TCATTAGTCA GCAAAGTGGG 
GAAGCAGAAA TATGTGTAAG ACTGGACCTC AATATGCTAT CAGATTCTGA AAATTCCCAG 
CATCTGGAAC TTCATGAGCC ACCAAAAGCT GTGGATAAGG CGAAATCCCC AGGGGTTGAT 
CCTAAGCAGT TGGCAGCAGA ACTCCAGAAG GTTTCACTAC AGCAGTCACC GCTGGTTCTG 
TCATCAGTTG TTGAAAAAGG ATCTCATGTT CATTCAGGTC CTACATCAGC AGGATCCAGT 
TCCGTTCCCA GCCCTGGGCA ACCAGGGTCC CCCTCAGTGA GCAAAAAGAA GCACGGCAGC 
AGCAAGCCTA CCGATGGAAC AAAGGTTGTC TCTCATCCAA TTACAGGAGA AATTCAGCTT 
CAAATTAACT ATGATCTTGG AAATCTCATA ATACATATTC TCCAAGCAAG AAATCTTGTT 
CCTCGAGACA ACAATGGTTA TTCTGACCCT TTTGTGAAAG TGTACCTTCT TCCAGGGAGA 
GGTCAAGTCA TGGTTGTCCA GAATGCAAGT GCTGAGTACA AGAGAAGGAC TAAACATGTC 
CAGAAAAGTC TTAATCCTGA GTGGAATCAA ACAGTAATTT ATAAAAGTAT TTCCATGGAA 
CAGCTCAAGA AGAAAACACT GGAGGTGACA GTTTGGGATT ATGATAGATT TTCATCCAAC 
GACTTCCTTG GGGAGGTATT GATTGATTTA TCTAGCACAT CTCACCTCGA TAACACTCCA 
AGGTGGTATC CTCTCAAAGA ACAGACTGAA AGCATTGATC ATGGCAAGTC TCATTCCAGT 
CAGAGCAGCC AGCAGTCCCC AAAGCCATCT GTTATCAAAA GCAGAAGCCA TGGTATCTTC 
CCTGACCCAT CAAAGGACAT GCAGGTTCCC ACCATTGAGA AATCCCATAG TAGTCCTGGT 
AGCTCAAAAT CATCATCAGA AGGCCATCTC CGTTCTCATG GACCATCTCG CAGTCAAAGC 
AAAACCAGCG TCACTCAGAC CCACCTGGAA GATGCAGGGG CTGCCATAGC TGCTGCCGAA 
GCTGCCGTGC AACAACTCCG CATTCAACCA ACAAAGCCTC CCAATCACCG GCCTGCAGAA 
AGCTCTGTGT CCACCGGCTC CTCGGGCAGC AGCTTTGGCA GTGGGTATAG CGTGGACAGT 
GAAGGAAGCA GCAGCACTGC AGGGGAGACT AATCTATTTC CTATTCCGAG GATAGGGAAG 
ATGGGACAGA ATGGACAAGA GCCTGTAAAA CAGCCAGGGG TAGGAGTAGG ACTAGCAGAC 
ACTGAAGCTA AAACTCAGGT AATGGGAGAA ATCAAGATTG CATTGAAGAA GGAAATGAAG 
ACAGATGGTG AACAACTAAT AGTTGAAATT CTCCAATGCA GAAATATTAC ATACAAATTT 
AAGTCTCCTG ATCATCTACC AGATTTATAT GTGAAAATAT ATGTGATGAA TATTTCTACC 
CAAAAAAAGG TGATCAAGAA AAAAACAAGA GTATGCAGAC ATGATCGAGA GCCTTCGTTT 
AATGAAACTT TTCGATTCAG TCTAAGTCCT GCAGGACATT CTCTTCAGAT TTTACTTTTC 
TCCAATGGAG GGAAGTTTAT GAAAAAGACC TTGATTGGTG AAGCCTGTAT CTGGCTTGAC 
AAAGTGGATC TCAGAAAAAG AATAGTCAAC TGGCACAAAC TTTTGGTCAG TCCTACTCAA 
ACGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCLO ( NM_033026.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_149015.2 Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant 1, mRNA.

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CloneID	OHu24277
Clone ID Related Accession (Same CDS sequence)	NM_033026.5 , NM_033026.6
Accession Version	NM_033026.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 15429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	protein piccolo isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093461.4, AC004903.2, AC004886.1, AC080093.6, AC004082.1 and AC004006.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_033026.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000333891.14/ ENSP00000334319.8 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA 
GCGGCTGGAG GAGGAGCTAG CGGGGCGGGG AGCCCCTCTC ACACCGCGAT CCCGGCCGGC 
ATGGAGGCGG ATTTGAGCCA GCTGAGCGAA GAGGAGAGGA GACAGATCGC CGCTGTCATG 
TCAAGGGCGC AGGGGCTGCC CAAGGGAAGC GTCCCCCCGG CCGCTGCGGA GTCGCCTTCC 
ATGCACAGGA AACAAGAGTT GGATAGTAGT CATCCTCCAA AGCAATCAGG AAGACCCCCG 
GACCCTGGGC GTCCAGCTCA ACCTGGTCTC AGTAAAAGTA GAACTACAGA CACTTTCAGG 
TCAGAGCAGA AATTGCCTGG GAGGAGTCCT TCCACTATTA GCTTGAAAGA ATCAAAGTCC 
AGAACTGATT TAAAGGAAGA GCACAAGTCT AGTATGATGC CTGGCTTCCT CTCAGAGGTT 
AACGCTTTAA GTGCTGTTTC CTCTGTTGTA AATAAATTCA ACCCTTTTGA TTTGATATCA 
GACTCTGAGG CATCCCAGGA AGAAACCACC AAGAAACAAA AAGTGGTTCA GAAGGAGCAA 
GGAAAACCTG AAGGAATCAT AAAACCTCCT TTACAACAAC AGCCACCCAA GCCGATTCCT 
AAGCAGCAAG GACCTGGTAG GGATCCGCTT CAGCAGGATG GCACTCCCAA ATCAATATCT 
TCTCAACAAC CAGAAAAAAT TAAATCACAA CCTCCAGGTA CAGGAAAGCC AATTCAGGGT 
CCTACCCAGA CTCCTCAGAC AGACCATGCA AAATTGCCAC TTCAACGAGA TGCATCCAGG 
CCTCAGACTA AACAGGCAGA CATAGTAAGG GGAGAATCAG TTAAACCCTC ACTGCCAAGC 
CCATCCAAAC CACCTATTCA GCAACCAACT CCTGGAAAAC CTCCAGCACA GCAGCCTGGA 
CATGAAAAAT CACAGCCTGG GCCTGCAAAG CCCCCAGCTC AGCCCTCAGG GCTAACAAAG 
CCATTGGCTC AACAACCAGG GACAGTGAAA CCCCCAGTCC AGCCACCAGG GACAACAAAG 
CCTCCAGCTC AGCCTCTTGG TCCTGCTAAG CCTCCAGCTC AGCAGACTGG GTCAGAGAAG 
CCTTCATCGG AGCAGCCTGG GCCAAAGGCT TTAGCTCAGC CTCCTGGAGT TGGAAAGACT 
CCAGCTCAAC AGCCAGGGCC AGCAAAGCCT CCAACCCAGC AGGTGGGGAC ACCAAAACCC 
CTAGCTCAAC AACCTGGGCT ACAGTCTCCA GCTAAGGCAC CTGGGCCTAC AAAGACTCCA 
GTTCAGCAGC CTGGGCCAGG AAAGATTCCA GCTCAACAGG CAGGACCCGG AAAGACTTCT 
GCCCAGCAGA CTGGCCCAAC AAAGCCTCCT TCACAACTGC CTGGCCCAGC AAAGCCCCCA 
CCTCAACAGC CTGGCCCAGC AAAGCCCCCA CCTCAACAGC CTGGCTCAGC AAAGCCCCCA 
TCTCAACAGC CTGGCTCAAC AAAACCCCCA CCTCAACAGC CTGGCCCAGC AAAGCCCTCA 
CCTCAACAGC CTGGCTCAAC AAAACCCCCA TCTCAACAGC CTGGCTCAGC AAAACCCTCA 
GCTCAACAGC CTAGCCCAGC AAAGCCCTCG GCTCAGCAAT CTACAAAACC AGTAAGCCAA 
ACAGGATCTG GAAAACCTCT GCAGCCACCA ACAGTGTCTC CATCTGCAAA ACAGCCTCCT 
TCACAAGGCC TCCCTAAAAC CATCTGTCCT CTTTGCAATA CCACTGAACT TCTGTTGCAT 
GTTCCAGAAA AGGCCAATTT TAACACATGC ACTGAGTGTC AAACCACTGT CTGTAGTCTC 
TGTGGTTTTA ATCCCAATCC TCATTTAACG GAGGTAAAAG AGTGGCTCTG TTTGAACTGT 
CAAATGAAAA GAGCTCTAGG CGGGGATCTG GCTCCAGTTC CGTCATCACC CCAGCCCAAA 
CTGAAGACTG CACCTGTTAC CACTACATCA GCAGTGAGCA AATCATCCCC ACAGCCACAG 
CAGACTTCCC CAAAGAAGGA TGCTGCACCA AAACAGGATC TCTCCAAGGC ACCTGAGCCT 
AAAAAGCCAC CACCACTAGT GAAACAACCA ACCCTTCATG GCTCTCCTTC AGCCAAGGCC 
AAGCAGCCCC CTGAGGCAGA TTCTTTGTCC AAGCCAGCCC CTCCCAAAGA ACCTTCTGTC 
CCATCTGAGC AGGACAAGGC CCCTGTTGCT GATGATAAAC CAAAGCAGCC CAAGATGGTA 
AAGCCAACCA CTGACCTTGT ATCTTCATCA TCAGCAACAA CAAAACCTGA TATTCCAAGC 
TCCAAAGTAC AGTCACAAGC TGAAGAGAAA ACAACCCCTC CTCTAAAAAC AGACTCTGCC 
AAACCCTCAC AGAGTTTTCC ACCAACAGGG GAAAAAGTCA GCCCATTTGA TTCTAAAGCC 
ATACCTCGAC CTGCATCAGA TTCAAAAATT ATTTCACATC CTGGTCCCAG TTCAGAGAGC 
AAAGGTCAAA AACAAGTTGA CCCCGTACAA AAGAAGGAAG AACCCAAGAA AGCACAAACC 
AAAATGAGTC CTAAACCAGA TGCCAAGCCA ATGCCAAAAG GGTCACCAAC ACCCCCTGGC 
CCACGACCTA CCGCTGGCCA AACTGTCCCC ACACCTCAAC AGTCCCCAAA GCCTCAGGAG 
CAGTCAAGGC GTTTCAGTCT GAATCTGGGA AGTATTACTG ATGCCCCCAA ATCACAGCCT 
ACAACTCCTC AAGAGACCGT GACTGGGAAA CTCTTTGGGT TTGGAGCATC AATCTTCAGC 
CAGGCATCAA ATTTAATTTC CACTGCAGGC CAACCTGGAC CTCATTCACA AAGTGGACCA 
GGGGCCCCAA TGAAACAAGC CCCTGCCCCT TCACAGCCAC CTACTTCACA AGGGCCACCC 
AAATCCACAG GTCAAGCACC ACCAGCACCT GCAAAAAGTA TACCTGTGAA AAAGGAAACA 
AAAGCCCCAG CAGCTGAAAA ATTAGAGCCC AAAGCTGAAC AAGCTCCAAC AGTAAAAAGA 
ACAGAAACAG AAAAAAAGCC ACCACCTATT AAGGATAGCA AATCTTTAAC AGCTGAGCCT 
CAAAAGGCTG TCCTTCCCAC AAAACTGGAG AAATCGCCCA AACCAGAATC AACCTGTCCT 
CTCTGCAAAA CTGAACTCAA CATAGGTTCT AAGGATCCTC CTAACTTCAA TACTTGCACT 
GAATGCAAGA ATCAAGTGTG TAATCTCTGT GGATTTAACC CTACACCACA TTTGACTGAG 
ATTCAAGAAT GGCTTTGTTT AAATTGCCAA ACCCAGAGAG CAATATCAGG ACAGCTTGGA 
GACATACGCA AAATGCCACC TGCACCATCA GGACCCAAAG CATCTCCTAT GCCTGTTCCT 
ACAGAATCAT CATCTCAGAA AACAGCAGTG CCTCCCCAAG TAAAATTAGT GAAAAAGCAA 
GAACAAGAAG TAAAAACGGA AGCTGAAAAA GTCATTCTGG AAAAAGTAAA GGAAACACTA 
TCAATGGAAA AAATTCCTCC TATGGTAACC ACAGATCAAA AACAAGAAGA GAGTAAACTA 
GAGAAAGACA AAGCTTCAGC TCTTCAAGAA AAAAAGCCAC TCCCTGAAGA AAAAAAACTA 
ATCCCTGAAG AAGAAAAGAT ACGTTCTGAA GAAAAAAAGC CACTCCTAGA AGAAAAAAAG 
CCAACCCCTG AAGACAAAAA GCTACTCCCA GAGGCAAAAA CATCAGCCCC AGAAGAACAG 
AAACATGACT TACTTAAATC TCAAGTACAA ATTGCTGAAG AAAAGCTTGA AGGCAGAGTG 
GCTCCAAAGA CAGTGCAAGA AGGGAAACAA CCACAGACCA AGATGGAAGG TTTACCATCT 
GGCACACCTC AGAGTTTACC TAAAGAAGAT GATAAGACAA CCAAAACAAT AAAAGAACAG 
CCACAGCCAC CATGCACAGC AAAACCTGAT CAGGTGGAAC CTGGGAAAGA AAAAACAGAA 
AAGGAAGATG ACAAATCAGA CACCTCAAGT TCTCAGCAGC CTAAAAGCCC CCAAGGTCTG 
AGCGACACGG GATATTCTTC CGATGGAATA TCAAGCTCAC TTGGTGAAAT TCCAAGTCTT 
ATTCCAACTG ATGAAAAGGA TATTCTCAAG GGACTCAAAA AGGACTCTTT TTCACAAGAA 
AGCAGCCCTT CCAGCCCCTC AGATTTGGCT AAGTTAGAAA GTACAGTCCT ATCTATTTTG 
GAAGCTCAAG CAAGTACACT TGCTGATGAA AAGTCAGAAA AGAAAACACA ACCCCATGAA 
GTTTCTCCTG AACAGCCTAA AGACCAAGAG AAAACTCAGA GTTTATCTGA AACCTTGGAA 
ATTACTATTT CAGAAGAGGA GATCAAAGAG AGTCAAGAAG AAAGGAAAGA CACTTTTAAA 
AAAGATAGCC AACAAGATAT TCCTTCCAGC AAGGACCATA AAGAGAAGTC TGAGTTTGTT 
GATGACATAA CTACTAGAAG AGAGCCTTAT GATTCAGTTG AAGAGAGTAG TGAAAGTGAA 
AACTCACCTG TTCCACAAAG AAAACGAAGA ACTAGTGTTG GCTCATCAAG CAGTGATGAG 
TATAAACAGG AAGACAGCCA AGGATCAGGG GAAGAGGAGG ACTTCATTCG AAAACAAATC 
ATAGAAATGA GTGCTGATGA AGATGCTTCA GGTTCTGAAG ATGATGAGTT CATCAGAAAC 
CAGCTCAAAG AGATTAGTAG CAGTACTGAG AGCCAGAAGA AGGAAGAAAC AAAGGGAAAA 
GGCAAAATAA CAGCAGGGAA ACACAGACGA CTGACTCGAA AAAGTAGCAC AAGCATTGAT 
GAAGATGCAG GAAGACGTCA CTCATGGCAT GATGAAGACG ATGAAGCATT TGATGAAAGT 
CCTGAACTTA AATACAGAGA AACTAAAAGT CAGGAAAGTG AAGAACTTGT AGTTACTGGA 
GGAGGAGGGC TACGCCGATT TAAAACAATT GAGCTCAACA GTACAATAGC AGATAAATAT 
TCTGCAGAGT CATCACAGAA AAAAACAAGT TTGTATTTTG ACGAAGAGCC AGAATTGGAA 
ATGGAAAGCC TGACAGACTC ACCTGAAGAT AGGTCAAGGG GAGAGGGATC TTCGAGTCTG 
CATGCTTCCA GCTTCACTCC TGGTACATCC CCTACATCAG TATCATCACT TGATGAGGAC 
AGTGACAGTA GCCCGAGTCA CAAAAAAGGA GAGAGCAAAC AGCAACGCAA AGCTCGGCAC 
AGACCACATG GCCCTCTTTT GCCTACTATT GAAGATTCTT CAGAGGAAGA AGAATTGAGA 
GAGGAAGAAG AATTATTAAA GGAGCAAGAA AAGCAGAGGG AAATAGAACA GCAACAAAGA 
AAGAGTTCTA GTAAAAAATC AAAGAAAGAC AAAGATGAAC TTCGAGCTCA GAGAAGAAGG 
GAAAGGCCAA AGACACCACC TAGTAATCTC TCTCCCATTG AAGATGCATC TCCGACAGAA 
GAGTTACGTC AGGCTGCAGA AATGGAGGAG CTCCATAGAT CTTCTTGTTC TGAATATTCA 
CCTAGCATAG AGTCAGACCC AGAAGGTTTT GAAATAAGCC CGGAAAAAAT AATAGAAGTA 
CAAAAAGTTT ATAAATTGCC CACAGCTGTT TCATTATACT CACCAACAGA TGAGCAATCT 
ATTATGCAGA AAGAAGGTAG CCAAAAGGCG TTAAAAAGTG CTGAGGAGAT GTATGAAGAA 
ATGATGCATA AAACACACAA ATACAAAGCT TTTCCAGCTG CAAATGAACG AGATGAAGTG 
TTTGAAAAAG AGCCTTTGTA TGGTGGGATG CTAATAGAGG ATTATATTTA TGAATCTTTA 
GTAGAAGACA CGTACAATGG ATCGGTAGAT GGCAGTCTGC TAACAAGGCA AGAAGAAGAA 
AATGGATTTA TGCAGCAGAA AGGAAGAGAG CAAAAGATAA GACTTTCAGA ACAGATTTAT 
GAAGATCCTA TGCAGAAAAT TACAGACCTC CAGAAAGAGT TTTATGAGTT AGAAAGCTTA 
CATTCTGTTG TGCCTCAGGA AGATATTGTT TCAAGCTCTT TTATCATCCC AGAAAGCCAT 
GAGATAGTGG ACCTGGGTAC TATGGTAACT TCTACAGAAG AAGAAAGGAA ACTACTAGAT 
GCTGATGCTG CCTATGAAGA ACTTATGAAG AGGCAACAGA TGCAATTAAC ACCTGGATCT 
AGCCCAACCC AGGCCCCCAT TGGTGAGGAT ATGACAGAGT CCACCATGGA CTTTGACAGA 
ATGCCAGATG CCTCTTTGAC ATCAAGTGTT CTCTCAGGAG CGTCTCTTAC AGATTCGACC 
AGCAGTGCAA CACTCTCTAT CCCAGATGTT AAAATAACCC AACATTTTTC AACAGAAGAA 
ATTGAGGATG AATATGTAAC CGATTATACA AGAGAAATTC AAGAGATAAT TGCCCATGAA 
TCGCTGATTT TGACCTACTC GGAGCCTTCA GAAAGTGCTA CATCTGTCCC ACCCTCTGAC 
ACACCTTCTC TCACATCATC TGTTTCTTCG GTCTGTACCA CAGATAGCTC TTCACCCATT 
ACTACCCTGG ATAGCATAAC CACAGTTTAT ACAGAGCCAG TGGACATGAT AACTAAATTT 
GAAGATTCTG AGGAAATTTC TTCATCAACT TATTTTCCAG GCAGCATTAT AGACTATCCA 
GAAGAAATAA GTGTATCTTT AGATCGGACT GCCCCACCAG ATGGTAGAGC TAGTGCTGAT 
CATATTGTTA TTTCCTTATC TGATATGGCA TCTTCTATCA TAGAATCTGT AGTACCTAAA 
CCTGAAGGGC CAGTTGCTGA CACTGTTTCT ACTGACTTAC TTATATCTGA AAAGGACCCA 
GTGAAGAAAG CCAAGAAGGA AACTGGGAAT GGAATCATTC TGGAAGTTTT GGAAGCTTAC 
AGAGATAAAA AGGAGTTGGA GGCCGAACGA ACAAAAAGTA GCTTATCCGA AACCGTGTTT 
GATCACCCAC CTTCTTCTGT AATAGCCCTT CCAATGAAAG AGCAGCTTTC AACTACATAC 
TTTACATCTG GAGAGACCTT TGGTCAGGAA AAACCTGCAT CTCAGTTACC ATCTGGCAGT 
CCTTCTGTTT CCTCTCTTCC AGCTAAACCT CGCCCATTCT TTAGAAGTTC TTCTTTGGAT 
ATATCAGCTC AACCTCCTCC CCCTCCTCCC CCTCCCCCTC CTCCTCCTCC TCCACCACCA 
CCCCCTCCTC CCCCACCACT TCCTCCACCA ACTTCACCTA AACCAACTAT TCTTCCTAAA 
AAAAAGTTAA CAGTTGCATC TCCAGTGACT ACAGCTACAC CTCTGTTTGA TGCTGTTACT 
ACTCTAGAGA CCACAGCTGT TCTGAGAAGT AATGGATTAC CTGTTACAAG AATATGTACT 
ACTGCACCTC CTCCTGTTCC TCCTAAGCCA TCTTCAATTC CATCTGGACT TGTATTTACC 
CACAGGCCTG AGCCAAGCAA ACCTCCAATC GCCCCCAAAC CAGTGATTCC TCAGCTTCCA 
ACAACTACAC AAAAACCAAC AGATATACAC CCCAAACCAA CAGGCCTATC TTTAACTTCA 
AGTATGACCT TAAATTTAGT GACTTCAGCA GATTATAAAT TGCCTTCCCC TACCTCCCCA 
CTTTCCCCAC ACTCCAACAA GTCTTCACCA AGATTTTCCA AATCCCTCAC AGAAACTTAT 
GTAGTTATTA CATTGCCATC TGAACCAGGG ACTCCAACAG ATTCTTCTGC TAGTCAAGCA 
ATTACCAGTT GGCCCTTGGG ATCACCCTCC AAAGATCTGG TTTCTGTTGA ACCTGTGTTT 
TCTGTAGTTC CTCCTGTGAC AGCTGTAGAA ATTCCAATTT CTTCAGAACA GACCTTCTAC 
ATCTCTGGAG CTTTACAGAC ATTTTCTGCT ACCCCTGTCA CAGCACCCTC TTCATTTCAA 
GCAGCTCCCA CATCAGTTAC ACAGTTTCTC ACTACTGAAG TTTCCAAGAC TGAGGTTTCA 
GCAACCAGAA GTACAGCTCC TAGTGTTGGT CTCAGCAGCA TTTCCATAAC AATTCCTCCA 
GAGCCTCTTG CTCTAGATAA CATACATTTA GAGAAGCCTC AGTATAAAGA AGATGGAAAA 
TTGCAACTTG TTGGTGATGT AATTGATTTG CGTACAGTAC CAAAGGTAGA AGTTAAAACA 
ACTGATAAAT GTATTGATCT TTCTGCTTCT ACAATGGATG TGAAAAGGCA GATCACAGCA 
AATGAAGTTT ATGGGAAACA AATTAGTGCT GTCCAACCCT CTATTATAAA TCTTAGTGTG 
ACATCATCAA TAGTGACTCC TGTATCTCTG GCCACTGAGA CAGTGACCTT TGTCACATGC 
ACAGCTAGTG CAAGTTACAC TACAGGCACA GAAAGCCTAG TGGGTGCAGA ACATGCAATG 
ACAACACCAC TCCAACTTAC AACATCAAAG CATGCTGAGC CCCCATACAG GATACCAAGT 
GACCAGGTCT TTCCTATAGC TAGGGAAGAA GCACCAATAA ACTTATCTCT AGGTACTCCA 
GCACATGCAG TGACATTGGC TATTACAAAA CCTGTCACTG TGCCTCCTGT TGGTGTCACA 
AATGGATGGA CTGATAGCAC CGTATCCCAG GGAATCACTG ATGGGGAAGT AGTGGATCTC 
AGTACAACCA AGTCTCACAG AACAGTCGTA ACAATGGATG AGTCTACTTC AAGTGTGATG 
ACCAAAATAA TAGAAGATGA AAAACCCGTT GATTTAACCG CAGGGAGAAG AGCTGTGTGC 
TGTGATGTGG TTTATAAATT ACCATTTGGA AGGAGCTGCA CAGCACAGCA GCCTGCAACT 
ACTCTTCCTG AGGATCGTTT TGGTTATAGG GATGACCACT ATCAGTATGA TCGATCAGGG 
CCATATGGTT ATAGAGGGAT TGGGGGAATG AAGCCTTCCA TGTCTGACAC AAATTTAGCA 
GAAGCTGGAC ATTTTTTCTA TAAAAGTAAG AATGCTTTTG ATTATTCTGA AGGAACTGAC 
ACAGCAGTAG ATCTGACTTC AGGGAGAGTT ACTACAGGTG AGGTAATGGA TTATTCAAGC 
AAGACTACAG GTCCATATCC AGAAACACGA CAAGTCATTT CAGGAGCTGG GATTAGTACC 
CCACAGTATT CCACAGCAAG AATGACACCA CCACCAGGAC CCCAGTATTG TGTGGGGAGT 
GTTTTGAGGT CATCTAATGG TGTTGTCTAT TCTTCAGTAG CAACTCCAAC ACCCTCTACA 
TTTGCTATCA CCACACAACC TGGCTCCATT TTCAGCACCA CAGTGAGGGA TTTGTCTGGT 
ATTCATACGG CTGATGCAGT GACTTCATTA CCTGCCATGC ACCATAGCCA GCCAATGCCT 
AGATCATATT TTATAACAAC AGGTGCATCT GAAACGGACA TTGCAGTAAC TGGTATTGAT 
ATCAGTGCCA GTTTGCAAAC TATTACTATG GAGTCTCTTA CTGCTGAGAC GATAGACTCT 
GTTCCCACTT TAACCACAGC ATCCGAAGTG TTTCCTGAAG TGGTGGGAGA TGAAAGTGCT 
CTTTTAATTG TCCCTGAAGA AGATAAACAA CAGCAGCAGC TAGACTTGGA GCGTGAGCTC 
CTGGAACTGG AGAAAATTAA GCAACAGCGC TTTGCTGAGG AATTGGAGTG GGAACGTCAG 
GAAATTCAAA GGTTCCGAGA ACAAGAAAAG ATCATGGTTC AGAAAAAGTT GGAGGAGCTG 
CAGTCTATGA AGCAACACCT TCTCTTTCAG CAAGAAGAAG AGCGGCAAGC CCAGTTCATG 
ATGAGGCAGG AGACGTTAGC TCAGCAACAG TTACAGCTTG AGCAGATCCA ACAGCTGCAA 
CAACAGCTTC ACCAGCAGCT GGAGGAGCAA AAGATTCGGC AGATCTACCA GTATAACTAT 
GACCCTTCTG GAACTGCTTC TCCACAAACC ACTACAGAGC AGGCAATTTT GGAAGGTCAG 
TATGCTGCTC TGGAAGGCAG TCAATTTTGG GCAACTGAAG ATGCAACCAC CACAGCTTCA 
GCTGTTGTGG CAATTGAAAT ACCACAAAGC CAAGGATGGT ACACCGTTCA GTCTGATGGT 
GTTACTCAGT ACATTGCCCC ACCTGGTATC CTGAGCACTG TTTCAGAAAT ACCTCTAACA 
GATGTTGTTG TGAAAGAGGA AAAACAACCC AAAAAGAGAA GTTCTGGAGC TAAAGTCCGA 
GGACAGTATG ATGACATGGG AGAAAATATG ACAGATGATC CCCGAAGTTT TAAAAAGATA 
GTGGACAGTG GTGTACAAAC GGATGACGAA GATGCCACAG ATCGGAGCTA TGTGAGTAGG 
AGAAGGAGAA CTAAAAAGAG TGTGGATACA AGCGTCCAAA CTGATGATGA AGATCAGGAT 
GAGTGGGATA TGCCTACTAG ATCAAGGAGG AAAGCTCGTG TAGGGAAATA TGGTGACAGC 
ATGACAGAGG CTGACAAGAC CAAACCCCTT TCCAAAGTCT CCAGCATAGC AGTTCAAACG 
GTAGCAGAGA TATCTGTGCA AACTGAACCA GTTGGAACCA TAAGAACACC CTCCATACGG 
GCACGAGTGG ATGCCAAGGT AGAAATAATT AAACACATTT CAGCACCTGA AAAGACTTAC 
AAAGGGGGCA GTTTAGGATG TCAAACAGAA GCAGATTCAG ACACACAAAG TCCTCAATAT 
CTGAGTGCCA CATCTCCACC CAAAGACAAG AAACGCCCAA CACCTTTAGA GATTGGTTAT 
TCATCTCACC TCCGGGCAGA TTCCACAGTA CAGCTGGCTC CTTCCCCACC CAAATCCCCC 
AAAGTCCTTT ACTCACCCAT CTCACCACTT TCACCAGGCA AAGCCTTAGA ATCAGCCTTT 
GTACCTTATG AAAAACCCCT CCCTGATGAT ATAAGTCCAC AGAAAGTACT GCATCCAGAT 
ATGGCTAAAG TTCCCCCAGC AAGTCCTAAG ACAGCCAAGA TGATGCAGCG TTCTATGTCT 
GACCCCAAGC CTCTGAGTCC AACAGCAGAC GAAAGTTCCA GGGCTCCTTT TCAGTATACC 
GAGGGCTATA CGACTAAAGG TTCTCAAACC ATGACATCCT CTGGAGCCCA GAAAAAAGTT 
AAAAGAACTC TGCCAAATCC ACCTCCTGAG GAGATTTCCA CAGGAACTCA ATCCACATTC 
AGCACAATGG GCACAGTTTC CAGGAGAAGG ATCTGCAGAA CCAACACAAT GGCACGAGCC 
AAGATTCTCC AGGACATAGA CAGAGAGCTT GATCTTGTGG AAAGGGAGTC TGCAAAACTT 
CGAAAGAAAC AAGCAGAGCT TGATGAAGAA GAAAAGGAGA TTGATGCTAA GCTACGATAC 
CTGGAAATGG GAATTAACAG GAGGAAAGAG GCCCTATTAA AGGAGAGAGA AAAGAGAGAA 
CGAGCCTACC TCCAGGGAGT AGCTGAGGAT CGTGATTACA TGTCTGACAG TGAAGTGAGT 
AGCACAAGAC CAACCCGAAT AGAAAGTCAG CATGGCATTG AGCGACCAAG AACTGCTCCC 
CAAACTGAAT TCAGCCAGTT TATACCACCA CAAACCCAAA CAGAATCTCA ACTAGTTCCT 
CCGACAAGTC CTTACACACA ATACCAGTAC TCTTCCCCTG CTCTTCCTAC CCAAGCACCC 
ACCTCATACA CTCAACAGTC TCATTTTGAG CAACAAACTT TGTACCATCA GCAAGTTTCA 
CCTTATCAGA CTCAGCCAAC ATTCCAAGCT GTGGCAACAA TGTCCTTCAC ACCTCAAGTT 
CAACCTACAC CAACCCCACA GCCTTCTTAT CAGTTACCTT CACAGATGAT GGTGATACAA 
CAGAAGCCAC GGCAAACTAC ATTATATTTG GAGCCCAAGA TAACCTCAAA CTATGAAGTG 
ATTCGCAACC AACCCCTTAT GATAGCACCT GTTTCTACGG ATAACACATT TGCTGTTTCC 
CATCTTGGTA GTAAGTACAA TAGTTTAGAC TTGAGAATAG GTTTGGAGGA AAGAAGTAGC 
ATGGCAAGCA GTCCAATATC AAGCATATCT GCAGATTCTT TCTATGCAGA TATTGATCAC 
CATACTCCAC GAAATTATGT CCTAATTGAC GACATTGGAG AGATCACCAA AGGAACAGCG 
GCATTAAGCA CCGCATTTAG CCTTCATGAA AAGGATCTGT CAAAAACAGA CCGTCTCCTT 
CGAACCACTG AGACACGCCG GTCTCAAGAA GTGACAGATT TCCTAGCACC TTTACAGTCT 
TCCTCTAGAT TGCATAGTTA TGTGAAGGCG GAGGAAGACC CAATGGAGGA TCCTTACGAG 
TTAAAGCTTC TGAAACATCA GATTAAACAG GAATTTCGTA GAGGGACAGA GAGCTTAGAT 
CACCTTGCTG GTCTTTCTCA TTATTACCAT GCTGATACTA GCTACAGACA TTTTCCAAAA 
TCTGAGAAGT ATAGCATCAG TAGACTCACA CTTGAAAAAC AAGCAGCAAA ACAACTGCCA 
GCAGCCATAC TTTATCAAAA GCAGTCAAAG CATAAGAAAT CACTAATTGA CCCTAAAATG 
TCAAAATTTT CACCTATTCA AGAAAGTAGA GACCTTGAAC CTGATTATTC AAGCTATATG 
ACTTCTAGCA CTTCATCTAT TGGTGGCATT TCCTCCAGGG CAAGGCTCCT TCAAGATGAC 
ATCACTTTTG GCCTCAGAAA AAATATTACA GACCAACAAA AATTTATGGG ATCTTCTCTT 
GGCACAGGAC TGGGCACATT AGGAAATACC ATACGCTCAG CTCTGCAGGA TGAAGCGGAT 
AAGCCATACA GTAGTGGCAG CAGGTCCAGA CCTTCCTCCA GACCTTCCTC TGTCTATGGG 
CTTGATTTAT CAATTAAAAG GGATTCTTCT AGCTCTTCCC TAAGACTGAA AGCTCAAGAG 
GCTGAAGCTC TAGATGTTTC CTTTAGTCAT GCATCATCCT CTGCCAGAAC TAAGCCGACC 
AGTTTGCCAA TTAGTCAAAG TAGAGGAAGA ATACCAATTG TGGCCCAGAA TTCTGAAGAA 
GAAAGCCCAC TCAGTCCTGT TGGCCAGCCA ATGGGAATGG CCAGGGCTGC AGCTGGACCC 
CTGCCACCAA TATCTGCAGA CACCAGGGAT CAGTTTGGAT CAAGCCACTC ATTGCCTGAA 
GTTCAGCAAC ACATGAGGGA AGAATCACGG ACTCGAGGCT ATGACCGTGA CATAGCATTC 
ATCATGGATG ACTTCCAACA TGCCATGTCA GACAGTGAAG CCTATCATCT GCGTCGTGAG 
GAAACAGATT GGTTTGATAA ACCCAGGGAG TCTCGTTTGG AAAATGGACA TGGTCTGGAC 
CGAAAACTGC CGGAAAGATT GGTCCACTCT AGACCACTCA GTCAACATCA AGAGCAAATT 
ATACAGATGA ACGGGAAAAC TATGCACTAC ATCTTTCCTC ACGCAAGGAT AAAAATAACA 
AGAGACTCAA AGGATCACAC AGTTTCAGGT AATGGATTAG GAATTAGAAT TGTGGGTGGT 
AAAGAAATCC CGGGACATAG TGGAGAAATT GGAGCCTATA TTGCCAAGAT TCTTCCTGGG 
GGAAGTGCGG AACAGACGGG GAAGCTTATG GAAGGGATGC AAGTATTGGA ATGGAATGGA 
ATTCCCTTGA CTTCTAAAAC ATATGAAGAA GTTCAGAGTA TCATTAGTCA GCAAAGTGGG 
GAAGCAGAAA TATGTGTAAG ACTGGACCTC AATATGCTAT CAGATTCTGA AAATTCCCAG 
CATCTGGAAC TTCATGAGCC ACCAAAAGCT GTGGATAAGG CGAAATCCCC AGGGGTTGAT 
CCTAAGCAGT TGGCAGCAGA ACTCCAGAAG GTTTCACTAC AGCAGTCACC GCTGGTTCTG 
TCATCAGTTG TTGAAAAAGG ATCTCATGTT CATTCAGGTC CTACATCAGC AGGATCCAGT 
TCCGTTCCCA GCCCTGGGCA ACCAGGGTCC CCCTCAGTGA GCAAAAAGAA GCACGGCAGC 
AGCAAGCCTA CCGATGGAAC AAAGGTTGTC TCTCATCCAA TTACAGGAGA AATTCAGCTT 
CAAATTAACT ATGATCTTGG AAATCTCATA ATACATATTC TCCAAGCAAG AAATCTTGTT 
CCTCGAGACA ACAATGGTTA TTCTGACCCT TTTGTGAAAG TGTACCTTCT TCCAGGGAGA 
GGTCAAGTCA TGGTTGTCCA GAATGCAAGT GCTGAGTACA AGAGAAGGAC TAAACATGTC 
CAGAAAAGTC TTAATCCTGA GTGGAATCAA ACAGTAATTT ATAAAAGTAT TTCCATGGAA 
CAGCTCAAGA AGAAAACACT GGAGGTGACA GTTTGGGATT ATGATAGATT TTCATCCAAC 
GACTTCCTTG GGGAGGTATT GATTGATTTA TCTAGCACAT CTCACCTCGA TAACACTCCA 
AGGTGGTATC CTCTCAAAGA ACAGACTGAA AGCATTGATC ATGGCAAGTC TCATTCCAGT 
CAGAGCAGCC AGCAGTCCCC AAAGCCATCT GTTATCAAAA GCAGAAGCCA TGGTATCTTC 
CCTGACCCAT CAAAGGACAT GCAGGTTCCC ACCATTGAGA AATCCCATAG TAGTCCTGGT 
AGCTCAAAAT CATCATCAGA AGGCCATCTC CGTTCTCATG GACCATCTCG CAGTCAAAGC 
AAAACCAGCG TCACTCAGAC CCACCTGGAA GATGCAGGGG CTGCCATAGC TGCTGCCGAA 
GCTGCCGTGC AACAACTCCG CATTCAACCA ACAAAGCCTC CCAATCACCG GCCTGCAGAA 
AGCTCTGTGT CCACCGGCTC CTCGGGCAGC AGCTTTGGCA GTGGGTATAG CGTGGACAGT 
GAAGGAAGCA GCAGCACTGC AGGGGAGACT AATCTATTTC CTATTCCGAG GATAGGGAAG 
ATGGGACAGA ATGGACAAGA GCCTGTAAAA CAGCCAGGGG TAGGAGTAGG ACTAGCAGAC 
ACTGAAGCTA AAACTCAGGT AATGGGAGAA ATCAAGATTG CATTGAAGAA GGAAATGAAG 
ACAGATGGTG AACAACTAAT AGTTGAAATT CTCCAATGCA GAAATATTAC ATACAAATTT 
AAGTCTCCTG ATCATCTACC AGATTTATAT GTGAAAATAT ATGTGATGAA TATTTCTACC 
CAAAAAAAGG TGATCAAGAA AAAAACAAGA GTATGCAGAC ATGATCGAGA GCCTTCGTTT 
AATGAAACTT TTCGATTCAG TCTAAGTCCT GCAGGACATT CTCTTCAGAT TTTACTTTTC 
TCCAATGGAG GGAAGTTTAT GAAAAAGACC TTGATTGGTG AAGCCTGTAT CTGGCTTGAC 
AAAGTGGATC TCAGAAAAAG AATAGTCAAC TGGCACAAAC TTTTGGTCAG TCCTACTCAA 
ACGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149015.2
CDS	293..15721
Translation	MGNEASLEGEGLPEGLAAAAAAGGGASGAGSPSHTAIPAGMEADLSQLSEEERRQIAAVMSRAQGLPKGSVPPAAAESPSMHRKQELDSSHPPKQSGRPPDPGRPAQPGLSKSRTTDTFRSEQKLPGRSPSTISLKESKSRTDLKEEHKSSMMPGFLSEVNALSAVSSVVNKFNPFDLISDSEASQEETTKKQKVVQKEQGKPEGIIKPPLQQQPPKPIPKQQGPGRDPLQQDGTPKSISSQQPEKIKSQPPGTGKPIQGPTQTPQTDHAKLPLQRDASRPQTKQADIVRGESVKPSLPSPSKPPIQQPTPGKPPAQQPGHEKSQPGPAKPPAQPSGLTKPLAQQPGTVKPPVQPPGTTKPPAQPLGPAKPPAQQTGSEKPSSEQPGPKALAQPPGVGKTPAQQPGPAKPPTQQVGTPKPLAQQPGLQSPAKAPGPTKTPVQQPGPGKIPAQQAGPGKTSAQQTGPTKPPSQLPGPAKPPPQQPGPAKPPPQQPGSAKPPSQQPGSTKPPPQQPGPAKPSPQQPGSTKPPSQQPGSAKPSAQQPSPAKPSAQQSTKPVSQTGSGKPLQPPTVSPSAKQPPSQGLPKTICPLCNTTELLLHVPEKANFNTCTECQTTVCSLCGFNPNPHLTEVKEWLCLNCQMKRALGGDLAPVPSSPQPKLKTAPVTTTSAVSKSSPQPQQTSPKKDAAPKQDLSKAPEPKKPPPLVKQPTLHGSPSAKAKQPPEADSLSKPAPPKEPSVPSEQDKAPVADDKPKQPKMVKPTTDLVSSSSATTKPDIPSSKVQSQAEEKTTPPLKTDSAKPSQSFPPTGEKVSPFDSKAIPRPASDSKIISHPGPSSESKGQKQVDPVQKKEEPKKAQTKMSPKPDAKPMPKGSPTPPGPRPTAGQTVPTPQQSPKPQEQSRRFSLNLGSITDAPKSQPTTPQETVTGKLFGFGASIFSQASNLISTAGQPGPHSQSGPGAPMKQAPAPSQPPTSQGPPKSTGQAPPAPAKSIPVKKETKAPAAEKLEPKAEQAPTVKRTETEKKPPPIKDSKSLTAEPQKAVLPTKLEKSPKPESTCPLCKTELNIGSKDPPNFNTCTECKNQVCNLCGFNPTPHLTEIQEWLCLNCQTQRAISGQLGDIRKMPPAPSGPKASPMPVPTESSSQKTAVPPQVKLVKKQEQEVKTEAEKVILEKVKETLSMEKIPPMVTTDQKQEESKLEKDKASALQEKKPLPEEKKLIPEEEKIRSEEKKPLLEEKKPTPEDKKLLPEAKTSAPEEQKHDLLKSQVQIAEEKLEGRVAPKTVQEGKQPQTKMEGLPSGTPQSLPKEDDKTTKTIKEQPQPPCTAKPDQVEPGKEKTEKEDDKSDTSSSQQPKSPQGLSDTGYSSDGISSSLGEIPSLIPTDEKDILKGLKKDSFSQESSPSSPSDLAKLESTVLSILEAQASTLADEKSEKKTQPHEVSPEQPKDQEKTQSLSETLEITISEEEIKESQEERKDTFKKDSQQDIPSSKDHKEKSEFVDDITTRREPYDSVEESSESENSPVPQRKRRTSVGSSSSDEYKQEDSQGSGEEEDFIRKQIIEMSADEDASGSEDDEFIRNQLKEISSSTESQKKEETKGKGKITAGKHRRLTRKSSTSIDEDAGRRHSWHDEDDEAFDESPELKYRETKSQESEELVVTGGGGLRRFKTIELNSTIADKYSAESSQKKTSLYFDEEPELEMESLTDSPEDRSRGEGSSSLHASSFTPGTSPTSVSSLDEDSDSSPSHKKGESKQQRKARHRPHGPLLPTIEDSSEEEELREEEELLKEQEKQREIEQQQRKSSSKKSKKDKDELRAQRRRERPKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSPSIESDPEGFEISPEKIIEVQKVYKLPTAVSLYSPTDEQSIMQKEGSQKALKSAEEMYEEMMHKTHKYKAFPAANERDEVFEKEPLYGGMLIEDYIYESLVEDTYNGSVDGSLLTRQEEENGFMQQKGREQKIRLSEQIYEDPMQKITDLQKEFYELESLHSVVPQEDIVSSSFIIPESHEIVDLGTMVTSTEEERKLLDADAAYEELMKRQQMQLTPGSSPTQAPIGEDMTESTMDFDRMPDASLTSSVLSGASLTDSTSSATLSIPDVKITQHFSTEEIEDEYVTDYTREIQEIIAHESLILTYSEPSESATSVPPSDTPSLTSSVSSVCTTDSSSPITTLDSITTVYTEPVDMITKFEDSEEISSSTYFPGSIIDYPEEISVSLDRTAPPDGRASADHIVISLSDMASSIIESVVPKPEGPVADTVSTDLLISEKDPVKKAKKETGNGIILEVLEAYRDKKELEAERTKSSLSETVFDHPPSSVIALPMKEQLSTTYFTSGETFGQEKPASQLPSGSPSVSSLPAKPRPFFRSSSLDISAQPPPPPPPPPPPPPPPPPPPPPPLPPPTSPKPTILPKKKLTVASPVTTATPLFDAVTTLETTAVLRSNGLPVTRICTTAPPPVPPKPSSIPSGLVFTHRPEPSKPPIAPKPVIPQLPTTTQKPTDIHPKPTGLSLTSSMTLNLVTSADYKLPSPTSPLSPHSNKSSPRFSKSLTETYVVITLPSEPGTPTDSSASQAITSWPLGSPSKDLVSVEPVFSVVPPVTAVEIPISSEQTFYISGALQTFSATPVTAPSSFQAAPTSVTQFLTTEVSKTEVSATRSTAPSVGLSSISITIPPEPLALDNIHLEKPQYKEDGKLQLVGDVIDLRTVPKVEVKTTDKCIDLSASTMDVKRQITANEVYGKQISAVQPSIINLSVTSSIVTPVSLATETVTFVTCTASASYTTGTESLVGAEHAMTTPLQLTTSKHAEPPYRIPSDQVFPIAREEAPINLSLGTPAHAVTLAITKPVTVPPVGVTNGWTDSTVSQGITDGEVVDLSTTKSHRTVVTMDESTSSVMTKIIEDEKPVDLTAGRRAVCCDVVYKLPFGRSCTAQQPATTLPEDRFGYRDDHYQYDRSGPYGYRGIGGMKPSMSDTNLAEAGHFFYKSKNAFDYSEGTDTAVDLTSGRVTTGEVMDYSSKTTGPYPETRQVISGAGISTPQYSTARMTPPPGPQYCVGSVLRSSNGVVYSSVATPTPSTFAITTQPGSIFSTTVRDLSGIHTADAVTSLPAMHHSQPMPRSYFITTGASETDIAVTGIDISASLQTITMESLTAETIDSVPTLTTASEVFPEVVGDESALLIVPEEDKQQQQLDLERELLELEKIKQQRFAEELEWERQEIQRFREQEKIMVQKKLEELQSMKQHLLFQQEEERQAQFMMRQETLAQQQLQLEQIQQLQQQLHQQLEEQKIRQIYQYNYDPSGTASPQTTTEQAILEGQYAALEGSQFWATEDATTTASAVVAIEIPQSQGWYTVQSDGVTQYIAPPGILSTVSEIPLTDVVVKEEKQPKKRSSGAKVRGQYDDMGENMTDDPRSFKKIVDSGVQTDDEDATDRSYVSRRRRTKKSVDTSVQTDDEDQDEWDMPTRSRRKARVGKYGDSMTEADKTKPLSKVSSIAVQTVAEISVQTEPVGTIRTPSIRARVDAKVEIIKHISAPEKTYKGGSLGCQTEADSDTQSPQYLSATSPPKDKKRPTPLEIGYSSHLRADSTVQLAPSPPKSPKVLYSPISPLSPGKALESAFVPYEKPLPDDISPQKVLHPDMAKVPPASPKTAKMMQRSMSDPKPLSPTADESSRAPFQYTEGYTTKGSQTMTSSGAQKKVKRTLPNPPPEEISTGTQSTFSTMGTVSRRRICRTNTMARAKILQDIDRELDLVERESAKLRKKQAELDEEEKEIDAKLRYLEMGINRRKEALLKEREKRERAYLQGVAEDRDYMSDSEVSSTRPTRIESQHGIERPRTAPQTEFSQFIPPQTQTESQLVPPTSPYTQYQYSSPALPTQAPTSYTQQSHFEQQTLYHQQVSPYQTQPTFQAVATMSFTPQVQPTPTPQPSYQLPSQMMVIQQKPRQTTLYLEPKITSNYEVIRNQPLMIAPVSTDNTFAVSHLGSKYNSLDLRIGLEERSSMASSPISSISADSFYADIDHHTPRNYVLIDDIGEITKGTAALSTAFSLHEKDLSKTDRLLRTTETRRSQEVTDFLAPLQSSSRLHSYVKAEEDPMEDPYELKLLKHQIKQEFRRGTESLDHLAGLSHYYHADTSYRHFPKSEKYSISRLTLEKQAAKQLPAAILYQKQSKHKKSLIDPKMSKFSPIQESRDLEPDYSSYMTSSTSSIGGISSRARLLQDDITFGLRKNITDQQKFMGSSLGTGLGTLGNTIRSALQDEADKPYSSGSRSRPSSRPSSVYGLDLSIKRDSSSSSLRLKAQEAEALDVSFSHASSSARTKPTSLPISQSRGRIPIVAQNSEEESPLSPVGQPMGMARAAAGPLPPISADTRDQFGSSHSLPEVQQHMREESRTRGYDRDIAFIMDDFQHAMSDSEAYHLRREETDWFDKPRESRLENGHGLDRKLPERLVHSRPLSQHQEQIIQMNGKTMHYIFPHARIKITRDSKDHTVSGNGLGIRIVGGKEIPGHSGEIGAYIAKILPGGSAEQTGKLMEGMQVLEWNGIPLTSKTYEEVQSIISQQSGEAEICVRLDLNMLSDSENSQHLELHEPPKAVDKAKSPGVDPKQLAAELQKVSLQQSPLVLSSVVEKGSHVHSGPTSAGSSSVPSPGQPGSPSVSKKKHGSSKPTDGTKVVSHPITGEIQLQINYDLGNLIIHILQARNLVPRDNNGYSDPFVKVYLLPGRGQVMVVQNASAEYKRRTKHVQKSLNPEWNQTVIYKSISMEQLKKKTLEVTVWDYDRFSSNDFLGEVLIDLSSTSHLDNTPRWYPLKEQTESIDHGKSHSSQSSQQSPKPSVIKSRSHGIFPDPSKDMQVPTIEKSHSSPGSSKSSSEGHLRSHGPSRSQSKTSVTQTHLEDAGAAIAAAEAAVQQLRIQPTKPPNHRPAESSVSTGSSGSSFGSGYSVDSEGSSSTAGETNLFPIPRIGKMGQNGQEPVKQPGVGVGLADTEAKTQVMGEIKIALKKEMKTDGEQLIVEILQCRNITYKFKSPDHLPDLYVKIYVMNISTQKKVIKKKTRVCRHDREPSFNETFRFSLSPAGHSLQILLFSNGGKFMKKTLIGEACIWLDKVDLRKRIVNWHKLLVSPTQTH

Target ORF information:

RefSeq Version	NM_033026.6
Organism	Homo sapiens(human)
Definition	Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033026.6

ORF Insert Sequence:

ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA 
GCGGCTGGAG GAGGAGCTAG CGGGGCGGGG AGCCCCTCTC ACACCGCGAT CCCGGCCGGC 
ATGGAGGCGG ATTTGAGCCA GCTGAGCGAA GAGGAGAGGA GACAGATCGC CGCTGTCATG 
TCAAGGGCGC AGGGGCTGCC CAAGGGAAGC GTCCCCCCGG CCGCTGCGGA GTCGCCTTCC 
ATGCACAGGA AACAAGAGTT GGATAGTAGT CATCCTCCAA AGCAATCAGG AAGACCCCCG 
GACCCTGGGC GTCCAGCTCA ACCTGGTCTC AGTAAAAGTA GAACTACAGA CACTTTCAGG 
TCAGAGCAGA AATTGCCTGG GAGGAGTCCT TCCACTATTA GCTTGAAAGA ATCAAAGTCC 
AGAACTGATT TAAAGGAAGA GCACAAGTCT AGTATGATGC CTGGCTTCCT CTCAGAGGTT 
AACGCTTTAA GTGCTGTTTC CTCTGTTGTA AATAAATTCA ACCCTTTTGA TTTGATATCA 
GACTCTGAGG CATCCCAGGA AGAAACCACC AAGAAACAAA AAGTGGTTCA GAAGGAGCAA 
GGAAAACCTG AAGGAATCAT AAAACCTCCT TTACAACAAC AGCCACCCAA GCCGATTCCT 
AAGCAGCAAG GACCTGGTAG GGATCCGCTT CAGCAGGATG GCACTCCCAA ATCAATATCT 
TCTCAACAAC CAGAAAAAAT TAAATCACAA CCTCCAGGTA CAGGAAAGCC AATTCAGGGT 
CCTACCCAGA CTCCTCAGAC AGACCATGCA AAATTGCCAC TTCAACGAGA TGCATCCAGG 
CCTCAGACTA AACAGGCAGA CATAGTAAGG GGAGAATCAG TTAAACCCTC ACTGCCAAGC 
CCATCCAAAC CACCTATTCA GCAACCAACT CCTGGAAAAC CTCCAGCACA GCAGCCTGGA 
CATGAAAAAT CACAGCCTGG GCCTGCAAAG CCCCCAGCTC AGCCCTCAGG GCTAACAAAG 
CCATTGGCTC AACAACCAGG GACAGTGAAA CCCCCAGTCC AGCCACCAGG GACAACAAAG 
CCTCCAGCTC AGCCTCTTGG TCCTGCTAAG CCTCCAGCTC AGCAGACTGG GTCAGAGAAG 
CCTTCATCGG AGCAGCCTGG GCCAAAGGCT TTAGCTCAGC CTCCTGGAGT TGGAAAGACT 
CCAGCTCAAC AGCCAGGGCC AGCAAAGCCT CCAACCCAGC AGGTGGGGAC ACCAAAACCC 
CTAGCTCAAC AACCTGGGCT ACAGTCTCCA GCTAAGGCAC CTGGGCCTAC AAAGACTCCA 
GTTCAGCAGC CTGGGCCAGG AAAGATTCCA GCTCAACAGG CAGGACCCGG AAAGACTTCT 
GCCCAGCAGA CTGGCCCAAC AAAGCCTCCT TCACAACTGC CTGGCCCAGC AAAGCCCCCA 
CCTCAACAGC CTGGCCCAGC AAAGCCCCCA CCTCAACAGC CTGGCTCAGC AAAGCCCCCA 
TCTCAACAGC CTGGCTCAAC AAAACCCCCA CCTCAACAGC CTGGCCCAGC AAAGCCCTCA 
CCTCAACAGC CTGGCTCAAC AAAACCCCCA TCTCAACAGC CTGGCTCAGC AAAACCCTCA 
GCTCAACAGC CTAGCCCAGC AAAGCCCTCG GCTCAGCAAT CTACAAAACC AGTAAGCCAA 
ACAGGATCTG GAAAACCTCT GCAGCCACCA ACAGTGTCTC CATCTGCAAA ACAGCCTCCT 
TCACAAGGCC TCCCTAAAAC CATCTGTCCT CTTTGCAATA CCACTGAACT TCTGTTGCAT 
GTTCCAGAAA AGGCCAATTT TAACACATGC ACTGAGTGTC AAACCACTGT CTGTAGTCTC 
TGTGGTTTTA ATCCCAATCC TCATTTAACG GAGGTAAAAG AGTGGCTCTG TTTGAACTGT 
CAAATGAAAA GAGCTCTAGG CGGGGATCTG GCTCCAGTTC CGTCATCACC CCAGCCCAAA 
CTGAAGACTG CACCTGTTAC CACTACATCA GCAGTGAGCA AATCATCCCC ACAGCCACAG 
CAGACTTCCC CAAAGAAGGA TGCTGCACCA AAACAGGATC TCTCCAAGGC ACCTGAGCCT 
AAAAAGCCAC CACCACTAGT GAAACAACCA ACCCTTCATG GCTCTCCTTC AGCCAAGGCC 
AAGCAGCCCC CTGAGGCAGA TTCTTTGTCC AAGCCAGCCC CTCCCAAAGA ACCTTCTGTC 
CCATCTGAGC AGGACAAGGC CCCTGTTGCT GATGATAAAC CAAAGCAGCC CAAGATGGTA 
AAGCCAACCA CTGACCTTGT ATCTTCATCA TCAGCAACAA CAAAACCTGA TATTCCAAGC 
TCCAAAGTAC AGTCACAAGC TGAAGAGAAA ACAACCCCTC CTCTAAAAAC AGACTCTGCC 
AAACCCTCAC AGAGTTTTCC ACCAACAGGG GAAAAAGTCA GCCCATTTGA TTCTAAAGCC 
ATACCTCGAC CTGCATCAGA TTCAAAAATT ATTTCACATC CTGGTCCCAG TTCAGAGAGC 
AAAGGTCAAA AACAAGTTGA CCCCGTACAA AAGAAGGAAG AACCCAAGAA AGCACAAACC 
AAAATGAGTC CTAAACCAGA TGCCAAGCCA ATGCCAAAAG GGTCACCAAC ACCCCCTGGC 
CCACGACCTA CCGCTGGCCA AACTGTCCCC ACACCTCAAC AGTCCCCAAA GCCTCAGGAG 
CAGTCAAGGC GTTTCAGTCT GAATCTGGGA AGTATTACTG ATGCCCCCAA ATCACAGCCT 
ACAACTCCTC AAGAGACCGT GACTGGGAAA CTCTTTGGGT TTGGAGCATC AATCTTCAGC 
CAGGCATCAA ATTTAATTTC CACTGCAGGC CAACCTGGAC CTCATTCACA AAGTGGACCA 
GGGGCCCCAA TGAAACAAGC CCCTGCCCCT TCACAGCCAC CTACTTCACA AGGGCCACCC 
AAATCCACAG GTCAAGCACC ACCAGCACCT GCAAAAAGTA TACCTGTGAA AAAGGAAACA 
AAAGCCCCAG CAGCTGAAAA ATTAGAGCCC AAAGCTGAAC AAGCTCCAAC AGTAAAAAGA 
ACAGAAACAG AAAAAAAGCC ACCACCTATT AAGGATAGCA AATCTTTAAC AGCTGAGCCT 
CAAAAGGCTG TCCTTCCCAC AAAACTGGAG AAATCGCCCA AACCAGAATC AACCTGTCCT 
CTCTGCAAAA CTGAACTCAA CATAGGTTCT AAGGATCCTC CTAACTTCAA TACTTGCACT 
GAATGCAAGA ATCAAGTGTG TAATCTCTGT GGATTTAACC CTACACCACA TTTGACTGAG 
ATTCAAGAAT GGCTTTGTTT AAATTGCCAA ACCCAGAGAG CAATATCAGG ACAGCTTGGA 
GACATACGCA AAATGCCACC TGCACCATCA GGACCCAAAG CATCTCCTAT GCCTGTTCCT 
ACAGAATCAT CATCTCAGAA AACAGCAGTG CCTCCCCAAG TAAAATTAGT GAAAAAGCAA 
GAACAAGAAG TAAAAACGGA AGCTGAAAAA GTCATTCTGG AAAAAGTAAA GGAAACACTA 
TCAATGGAAA AAATTCCTCC TATGGTAACC ACAGATCAAA AACAAGAAGA GAGTAAACTA 
GAGAAAGACA AAGCTTCAGC TCTTCAAGAA AAAAAGCCAC TCCCTGAAGA AAAAAAACTA 
ATCCCTGAAG AAGAAAAGAT ACGTTCTGAA GAAAAAAAGC CACTCCTAGA AGAAAAAAAG 
CCAACCCCTG AAGACAAAAA GCTACTCCCA GAGGCAAAAA CATCAGCCCC AGAAGAACAG 
AAACATGACT TACTTAAATC TCAAGTACAA ATTGCTGAAG AAAAGCTTGA AGGCAGAGTG 
GCTCCAAAGA CAGTGCAAGA AGGGAAACAA CCACAGACCA AGATGGAAGG TTTACCATCT 
GGCACACCTC AGAGTTTACC TAAAGAAGAT GATAAGACAA CCAAAACAAT AAAAGAACAG 
CCACAGCCAC CATGCACAGC AAAACCTGAT CAGGTGGAAC CTGGGAAAGA AAAAACAGAA 
AAGGAAGATG ACAAATCAGA CACCTCAAGT TCTCAGCAGC CTAAAAGCCC CCAAGGTCTG 
AGCGACACGG GATATTCTTC CGATGGAATA TCAAGCTCAC TTGGTGAAAT TCCAAGTCTT 
ATTCCAACTG ATGAAAAGGA TATTCTCAAG GGACTCAAAA AGGACTCTTT TTCACAAGAA 
AGCAGCCCTT CCAGCCCCTC AGATTTGGCT AAGTTAGAAA GTACAGTCCT ATCTATTTTG 
GAAGCTCAAG CAAGTACACT TGCTGATGAA AAGTCAGAAA AGAAAACACA ACCCCATGAA 
GTTTCTCCTG AACAGCCTAA AGACCAAGAG AAAACTCAGA GTTTATCTGA AACCTTGGAA 
ATTACTATTT CAGAAGAGGA GATCAAAGAG AGTCAAGAAG AAAGGAAAGA CACTTTTAAA 
AAAGATAGCC AACAAGATAT TCCTTCCAGC AAGGACCATA AAGAGAAGTC TGAGTTTGTT 
GATGACATAA CTACTAGAAG AGAGCCTTAT GATTCAGTTG AAGAGAGTAG TGAAAGTGAA 
AACTCACCTG TTCCACAAAG AAAACGAAGA ACTAGTGTTG GCTCATCAAG CAGTGATGAG 
TATAAACAGG AAGACAGCCA AGGATCAGGG GAAGAGGAGG ACTTCATTCG AAAACAAATC 
ATAGAAATGA GTGCTGATGA AGATGCTTCA GGTTCTGAAG ATGATGAGTT CATCAGAAAC 
CAGCTCAAAG AGATTAGTAG CAGTACTGAG AGCCAGAAGA AGGAAGAAAC AAAGGGAAAA 
GGCAAAATAA CAGCAGGGAA ACACAGACGA CTGACTCGAA AAAGTAGCAC AAGCATTGAT 
GAAGATGCAG GAAGACGTCA CTCATGGCAT GATGAAGACG ATGAAGCATT TGATGAAAGT 
CCTGAACTTA AATACAGAGA AACTAAAAGT CAGGAAAGTG AAGAACTTGT AGTTACTGGA 
GGAGGAGGGC TACGCCGATT TAAAACAATT GAGCTCAACA GTACAATAGC AGATAAATAT 
TCTGCAGAGT CATCACAGAA AAAAACAAGT TTGTATTTTG ACGAAGAGCC AGAATTGGAA 
ATGGAAAGCC TGACAGACTC ACCTGAAGAT AGGTCAAGGG GAGAGGGATC TTCGAGTCTG 
CATGCTTCCA GCTTCACTCC TGGTACATCC CCTACATCAG TATCATCACT TGATGAGGAC 
AGTGACAGTA GCCCGAGTCA CAAAAAAGGA GAGAGCAAAC AGCAACGCAA AGCTCGGCAC 
AGACCACATG GCCCTCTTTT GCCTACTATT GAAGATTCTT CAGAGGAAGA AGAATTGAGA 
GAGGAAGAAG AATTATTAAA GGAGCAAGAA AAGCAGAGGG AAATAGAACA GCAACAAAGA 
AAGAGTTCTA GTAAAAAATC AAAGAAAGAC AAAGATGAAC TTCGAGCTCA GAGAAGAAGG 
GAAAGGCCAA AGACACCACC TAGTAATCTC TCTCCCATTG AAGATGCATC TCCGACAGAA 
GAGTTACGTC AGGCTGCAGA AATGGAGGAG CTCCATAGAT CTTCTTGTTC TGAATATTCA 
CCTAGCATAG AGTCAGACCC AGAAGGTTTT GAAATAAGCC CGGAAAAAAT AATAGAAGTA 
CAAAAAGTTT ATAAATTGCC CACAGCTGTT TCATTATACT CACCAACAGA TGAGCAATCT 
ATTATGCAGA AAGAAGGTAG CCAAAAGGCG TTAAAAAGTG CTGAGGAGAT GTATGAAGAA 
ATGATGCATA AAACACACAA ATACAAAGCT TTTCCAGCTG CAAATGAACG AGATGAAGTG 
TTTGAAAAAG AGCCTTTGTA TGGTGGGATG CTAATAGAGG ATTATATTTA TGAATCTTTA 
GTAGAAGACA CGTACAATGG ATCGGTAGAT GGCAGTCTGC TAACAAGGCA AGAAGAAGAA 
AATGGATTTA TGCAGCAGAA AGGAAGAGAG CAAAAGATAA GACTTTCAGA ACAGATTTAT 
GAAGATCCTA TGCAGAAAAT TACAGACCTC CAGAAAGAGT TTTATGAGTT AGAAAGCTTA 
CATTCTGTTG TGCCTCAGGA AGATATTGTT TCAAGCTCTT TTATCATCCC AGAAAGCCAT 
GAGATAGTGG ACCTGGGTAC TATGGTAACT TCTACAGAAG AAGAAAGGAA ACTACTAGAT 
GCTGATGCTG CCTATGAAGA ACTTATGAAG AGGCAACAGA TGCAATTAAC ACCTGGATCT 
AGCCCAACCC AGGCCCCCAT TGGTGAGGAT ATGACAGAGT CCACCATGGA CTTTGACAGA 
ATGCCAGATG CCTCTTTGAC ATCAAGTGTT CTCTCAGGAG CGTCTCTTAC AGATTCGACC 
AGCAGTGCAA CACTCTCTAT CCCAGATGTT AAAATAACCC AACATTTTTC AACAGAAGAA 
ATTGAGGATG AATATGTAAC CGATTATACA AGAGAAATTC AAGAGATAAT TGCCCATGAA 
TCGCTGATTT TGACCTACTC GGAGCCTTCA GAAAGTGCTA CATCTGTCCC ACCCTCTGAC 
ACACCTTCTC TCACATCATC TGTTTCTTCG GTCTGTACCA CAGATAGCTC TTCACCCATT 
ACTACCCTGG ATAGCATAAC CACAGTTTAT ACAGAGCCAG TGGACATGAT AACTAAATTT 
GAAGATTCTG AGGAAATTTC TTCATCAACT TATTTTCCAG GCAGCATTAT AGACTATCCA 
GAAGAAATAA GTGTATCTTT AGATCGGACT GCCCCACCAG ATGGTAGAGC TAGTGCTGAT 
CATATTGTTA TTTCCTTATC TGATATGGCA TCTTCTATCA TAGAATCTGT AGTACCTAAA 
CCTGAAGGGC CAGTTGCTGA CACTGTTTCT ACTGACTTAC TTATATCTGA AAAGGACCCA 
GTGAAGAAAG CCAAGAAGGA AACTGGGAAT GGAATCATTC TGGAAGTTTT GGAAGCTTAC 
AGAGATAAAA AGGAGTTGGA GGCCGAACGA ACAAAAAGTA GCTTATCCGA AACCGTGTTT 
GATCACCCAC CTTCTTCTGT AATAGCCCTT CCAATGAAAG AGCAGCTTTC AACTACATAC 
TTTACATCTG GAGAGACCTT TGGTCAGGAA AAACCTGCAT CTCAGTTACC ATCTGGCAGT 
CCTTCTGTTT CCTCTCTTCC AGCTAAACCT CGCCCATTCT TTAGAAGTTC TTCTTTGGAT 
ATATCAGCTC AACCTCCTCC CCCTCCTCCC CCTCCCCCTC CTCCTCCTCC TCCACCACCA 
CCCCCTCCTC CCCCACCACT TCCTCCACCA ACTTCACCTA AACCAACTAT TCTTCCTAAA 
AAAAAGTTAA CAGTTGCATC TCCAGTGACT ACAGCTACAC CTCTGTTTGA TGCTGTTACT 
ACTCTAGAGA CCACAGCTGT TCTGAGAAGT AATGGATTAC CTGTTACAAG AATATGTACT 
ACTGCACCTC CTCCTGTTCC TCCTAAGCCA TCTTCAATTC CATCTGGACT TGTATTTACC 
CACAGGCCTG AGCCAAGCAA ACCTCCAATC GCCCCCAAAC CAGTGATTCC TCAGCTTCCA 
ACAACTACAC AAAAACCAAC AGATATACAC CCCAAACCAA CAGGCCTATC TTTAACTTCA 
AGTATGACCT TAAATTTAGT GACTTCAGCA GATTATAAAT TGCCTTCCCC TACCTCCCCA 
CTTTCCCCAC ACTCCAACAA GTCTTCACCA AGATTTTCCA AATCCCTCAC AGAAACTTAT 
GTAGTTATTA CATTGCCATC TGAACCAGGG ACTCCAACAG ATTCTTCTGC TAGTCAAGCA 
ATTACCAGTT GGCCCTTGGG ATCACCCTCC AAAGATCTGG TTTCTGTTGA ACCTGTGTTT 
TCTGTAGTTC CTCCTGTGAC AGCTGTAGAA ATTCCAATTT CTTCAGAACA GACCTTCTAC 
ATCTCTGGAG CTTTACAGAC ATTTTCTGCT ACCCCTGTCA CAGCACCCTC TTCATTTCAA 
GCAGCTCCCA CATCAGTTAC ACAGTTTCTC ACTACTGAAG TTTCCAAGAC TGAGGTTTCA 
GCAACCAGAA GTACAGCTCC TAGTGTTGGT CTCAGCAGCA TTTCCATAAC AATTCCTCCA 
GAGCCTCTTG CTCTAGATAA CATACATTTA GAGAAGCCTC AGTATAAAGA AGATGGAAAA 
TTGCAACTTG TTGGTGATGT AATTGATTTG CGTACAGTAC CAAAGGTAGA AGTTAAAACA 
ACTGATAAAT GTATTGATCT TTCTGCTTCT ACAATGGATG TGAAAAGGCA GATCACAGCA 
AATGAAGTTT ATGGGAAACA AATTAGTGCT GTCCAACCCT CTATTATAAA TCTTAGTGTG 
ACATCATCAA TAGTGACTCC TGTATCTCTG GCCACTGAGA CAGTGACCTT TGTCACATGC 
ACAGCTAGTG CAAGTTACAC TACAGGCACA GAAAGCCTAG TGGGTGCAGA ACATGCAATG 
ACAACACCAC TCCAACTTAC AACATCAAAG CATGCTGAGC CCCCATACAG GATACCAAGT 
GACCAGGTCT TTCCTATAGC TAGGGAAGAA GCACCAATAA ACTTATCTCT AGGTACTCCA 
GCACATGCAG TGACATTGGC TATTACAAAA CCTGTCACTG TGCCTCCTGT TGGTGTCACA 
AATGGATGGA CTGATAGCAC CGTATCCCAG GGAATCACTG ATGGGGAAGT AGTGGATCTC 
AGTACAACCA AGTCTCACAG AACAGTCGTA ACAATGGATG AGTCTACTTC AAGTGTGATG 
ACCAAAATAA TAGAAGATGA AAAACCCGTT GATTTAACCG CAGGGAGAAG AGCTGTGTGC 
TGTGATGTGG TTTATAAATT ACCATTTGGA AGGAGCTGCA CAGCACAGCA GCCTGCAACT 
ACTCTTCCTG AGGATCGTTT TGGTTATAGG GATGACCACT ATCAGTATGA TCGATCAGGG 
CCATATGGTT ATAGAGGGAT TGGGGGAATG AAGCCTTCCA TGTCTGACAC AAATTTAGCA 
GAAGCTGGAC ATTTTTTCTA TAAAAGTAAG AATGCTTTTG ATTATTCTGA AGGAACTGAC 
ACAGCAGTAG ATCTGACTTC AGGGAGAGTT ACTACAGGTG AGGTAATGGA TTATTCAAGC 
AAGACTACAG GTCCATATCC AGAAACACGA CAAGTCATTT CAGGAGCTGG GATTAGTACC 
CCACAGTATT CCACAGCAAG AATGACACCA CCACCAGGAC CCCAGTATTG TGTGGGGAGT 
GTTTTGAGGT CATCTAATGG TGTTGTCTAT TCTTCAGTAG CAACTCCAAC ACCCTCTACA 
TTTGCTATCA CCACACAACC TGGCTCCATT TTCAGCACCA CAGTGAGGGA TTTGTCTGGT 
ATTCATACGG CTGATGCAGT GACTTCATTA CCTGCCATGC ACCATAGCCA GCCAATGCCT 
AGATCATATT TTATAACAAC AGGTGCATCT GAAACGGACA TTGCAGTAAC TGGTATTGAT 
ATCAGTGCCA GTTTGCAAAC TATTACTATG GAGTCTCTTA CTGCTGAGAC GATAGACTCT 
GTTCCCACTT TAACCACAGC ATCCGAAGTG TTTCCTGAAG TGGTGGGAGA TGAAAGTGCT 
CTTTTAATTG TCCCTGAAGA AGATAAACAA CAGCAGCAGC TAGACTTGGA GCGTGAGCTC 
CTGGAACTGG AGAAAATTAA GCAACAGCGC TTTGCTGAGG AATTGGAGTG GGAACGTCAG 
GAAATTCAAA GGTTCCGAGA ACAAGAAAAG ATCATGGTTC AGAAAAAGTT GGAGGAGCTG 
CAGTCTATGA AGCAACACCT TCTCTTTCAG CAAGAAGAAG AGCGGCAAGC CCAGTTCATG 
ATGAGGCAGG AGACGTTAGC TCAGCAACAG TTACAGCTTG AGCAGATCCA ACAGCTGCAA 
CAACAGCTTC ACCAGCAGCT GGAGGAGCAA AAGATTCGGC AGATCTACCA GTATAACTAT 
GACCCTTCTG GAACTGCTTC TCCACAAACC ACTACAGAGC AGGCAATTTT GGAAGGTCAG 
TATGCTGCTC TGGAAGGCAG TCAATTTTGG GCAACTGAAG ATGCAACCAC CACAGCTTCA 
GCTGTTGTGG CAATTGAAAT ACCACAAAGC CAAGGATGGT ACACCGTTCA GTCTGATGGT 
GTTACTCAGT ACATTGCCCC ACCTGGTATC CTGAGCACTG TTTCAGAAAT ACCTCTAACA 
GATGTTGTTG TGAAAGAGGA AAAACAACCC AAAAAGAGAA GTTCTGGAGC TAAAGTCCGA 
GGACAGTATG ATGACATGGG AGAAAATATG ACAGATGATC CCCGAAGTTT TAAAAAGATA 
GTGGACAGTG GTGTACAAAC GGATGACGAA GATGCCACAG ATCGGAGCTA TGTGAGTAGG 
AGAAGGAGAA CTAAAAAGAG TGTGGATACA AGCGTCCAAA CTGATGATGA AGATCAGGAT 
GAGTGGGATA TGCCTACTAG ATCAAGGAGG AAAGCTCGTG TAGGGAAATA TGGTGACAGC 
ATGACAGAGG CTGACAAGAC CAAACCCCTT TCCAAAGTCT CCAGCATAGC AGTTCAAACG 
GTAGCAGAGA TATCTGTGCA AACTGAACCA GTTGGAACCA TAAGAACACC CTCCATACGG 
GCACGAGTGG ATGCCAAGGT AGAAATAATT AAACACATTT CAGCACCTGA AAAGACTTAC 
AAAGGGGGCA GTTTAGGATG TCAAACAGAA GCAGATTCAG ACACACAAAG TCCTCAATAT 
CTGAGTGCCA CATCTCCACC CAAAGACAAG AAACGCCCAA CACCTTTAGA GATTGGTTAT 
TCATCTCACC TCCGGGCAGA TTCCACAGTA CAGCTGGCTC CTTCCCCACC CAAATCCCCC 
AAAGTCCTTT ACTCACCCAT CTCACCACTT TCACCAGGCA AAGCCTTAGA ATCAGCCTTT 
GTACCTTATG AAAAACCCCT CCCTGATGAT ATAAGTCCAC AGAAAGTACT GCATCCAGAT 
ATGGCTAAAG TTCCCCCAGC AAGTCCTAAG ACAGCCAAGA TGATGCAGCG TTCTATGTCT 
GACCCCAAGC CTCTGAGTCC AACAGCAGAC GAAAGTTCCA GGGCTCCTTT TCAGTATACC 
GAGGGCTATA CGACTAAAGG TTCTCAAACC ATGACATCCT CTGGAGCCCA GAAAAAAGTT 
AAAAGAACTC TGCCAAATCC ACCTCCTGAG GAGATTTCCA CAGGAACTCA ATCCACATTC 
AGCACAATGG GCACAGTTTC CAGGAGAAGG ATCTGCAGAA CCAACACAAT GGCACGAGCC 
AAGATTCTCC AGGACATAGA CAGAGAGCTT GATCTTGTGG AAAGGGAGTC TGCAAAACTT 
CGAAAGAAAC AAGCAGAGCT TGATGAAGAA GAAAAGGAGA TTGATGCTAA GCTACGATAC 
CTGGAAATGG GAATTAACAG GAGGAAAGAG GCCCTATTAA AGGAGAGAGA AAAGAGAGAA 
CGAGCCTACC TCCAGGGAGT AGCTGAGGAT CGTGATTACA TGTCTGACAG TGAAGTGAGT 
AGCACAAGAC CAACCCGAAT AGAAAGTCAG CATGGCATTG AGCGACCAAG AACTGCTCCC 
CAAACTGAAT TCAGCCAGTT TATACCACCA CAAACCCAAA CAGAATCTCA ACTAGTTCCT 
CCGACAAGTC CTTACACACA ATACCAGTAC TCTTCCCCTG CTCTTCCTAC CCAAGCACCC 
ACCTCATACA CTCAACAGTC TCATTTTGAG CAACAAACTT TGTACCATCA GCAAGTTTCA 
CCTTATCAGA CTCAGCCAAC ATTCCAAGCT GTGGCAACAA TGTCCTTCAC ACCTCAAGTT 
CAACCTACAC CAACCCCACA GCCTTCTTAT CAGTTACCTT CACAGATGAT GGTGATACAA 
CAGAAGCCAC GGCAAACTAC ATTATATTTG GAGCCCAAGA TAACCTCAAA CTATGAAGTG 
ATTCGCAACC AACCCCTTAT GATAGCACCT GTTTCTACGG ATAACACATT TGCTGTTTCC 
CATCTTGGTA GTAAGTACAA TAGTTTAGAC TTGAGAATAG GTTTGGAGGA AAGAAGTAGC 
ATGGCAAGCA GTCCAATATC AAGCATATCT GCAGATTCTT TCTATGCAGA TATTGATCAC 
CATACTCCAC GAAATTATGT CCTAATTGAC GACATTGGAG AGATCACCAA AGGAACAGCG 
GCATTAAGCA CCGCATTTAG CCTTCATGAA AAGGATCTGT CAAAAACAGA CCGTCTCCTT 
CGAACCACTG AGACACGCCG GTCTCAAGAA GTGACAGATT TCCTAGCACC TTTACAGTCT 
TCCTCTAGAT TGCATAGTTA TGTGAAGGCG GAGGAAGACC CAATGGAGGA TCCTTACGAG 
TTAAAGCTTC TGAAACATCA GATTAAACAG GAATTTCGTA GAGGGACAGA GAGCTTAGAT 
CACCTTGCTG GTCTTTCTCA TTATTACCAT GCTGATACTA GCTACAGACA TTTTCCAAAA 
TCTGAGAAGT ATAGCATCAG TAGACTCACA CTTGAAAAAC AAGCAGCAAA ACAACTGCCA 
GCAGCCATAC TTTATCAAAA GCAGTCAAAG CATAAGAAAT CACTAATTGA CCCTAAAATG 
TCAAAATTTT CACCTATTCA AGAAAGTAGA GACCTTGAAC CTGATTATTC AAGCTATATG 
ACTTCTAGCA CTTCATCTAT TGGTGGCATT TCCTCCAGGG CAAGGCTCCT TCAAGATGAC 
ATCACTTTTG GCCTCAGAAA AAATATTACA GACCAACAAA AATTTATGGG ATCTTCTCTT 
GGCACAGGAC TGGGCACATT AGGAAATACC ATACGCTCAG CTCTGCAGGA TGAAGCGGAT 
AAGCCATACA GTAGTGGCAG CAGGTCCAGA CCTTCCTCCA GACCTTCCTC TGTCTATGGG 
CTTGATTTAT CAATTAAAAG GGATTCTTCT AGCTCTTCCC TAAGACTGAA AGCTCAAGAG 
GCTGAAGCTC TAGATGTTTC CTTTAGTCAT GCATCATCCT CTGCCAGAAC TAAGCCGACC 
AGTTTGCCAA TTAGTCAAAG TAGAGGAAGA ATACCAATTG TGGCCCAGAA TTCTGAAGAA 
GAAAGCCCAC TCAGTCCTGT TGGCCAGCCA ATGGGAATGG CCAGGGCTGC AGCTGGACCC 
CTGCCACCAA TATCTGCAGA CACCAGGGAT CAGTTTGGAT CAAGCCACTC ATTGCCTGAA 
GTTCAGCAAC ACATGAGGGA AGAATCACGG ACTCGAGGCT ATGACCGTGA CATAGCATTC 
ATCATGGATG ACTTCCAACA TGCCATGTCA GACAGTGAAG CCTATCATCT GCGTCGTGAG 
GAAACAGATT GGTTTGATAA ACCCAGGGAG TCTCGTTTGG AAAATGGACA TGGTCTGGAC 
CGAAAACTGC CGGAAAGATT GGTCCACTCT AGACCACTCA GTCAACATCA AGAGCAAATT 
ATACAGATGA ACGGGAAAAC TATGCACTAC ATCTTTCCTC ACGCAAGGAT AAAAATAACA 
AGAGACTCAA AGGATCACAC AGTTTCAGGT AATGGATTAG GAATTAGAAT TGTGGGTGGT 
AAAGAAATCC CGGGACATAG TGGAGAAATT GGAGCCTATA TTGCCAAGAT TCTTCCTGGG 
GGAAGTGCGG AACAGACGGG GAAGCTTATG GAAGGGATGC AAGTATTGGA ATGGAATGGA 
ATTCCCTTGA CTTCTAAAAC ATATGAAGAA GTTCAGAGTA TCATTAGTCA GCAAAGTGGG 
GAAGCAGAAA TATGTGTAAG ACTGGACCTC AATATGCTAT CAGATTCTGA AAATTCCCAG 
CATCTGGAAC TTCATGAGCC ACCAAAAGCT GTGGATAAGG CGAAATCCCC AGGGGTTGAT 
CCTAAGCAGT TGGCAGCAGA ACTCCAGAAG GTTTCACTAC AGCAGTCACC GCTGGTTCTG 
TCATCAGTTG TTGAAAAAGG ATCTCATGTT CATTCAGGTC CTACATCAGC AGGATCCAGT 
TCCGTTCCCA GCCCTGGGCA ACCAGGGTCC CCCTCAGTGA GCAAAAAGAA GCACGGCAGC 
AGCAAGCCTA CCGATGGAAC AAAGGTTGTC TCTCATCCAA TTACAGGAGA AATTCAGCTT 
CAAATTAACT ATGATCTTGG AAATCTCATA ATACATATTC TCCAAGCAAG AAATCTTGTT 
CCTCGAGACA ACAATGGTTA TTCTGACCCT TTTGTGAAAG TGTACCTTCT TCCAGGGAGA 
GGTCAAGTCA TGGTTGTCCA GAATGCAAGT GCTGAGTACA AGAGAAGGAC TAAACATGTC 
CAGAAAAGTC TTAATCCTGA GTGGAATCAA ACAGTAATTT ATAAAAGTAT TTCCATGGAA 
CAGCTCAAGA AGAAAACACT GGAGGTGACA GTTTGGGATT ATGATAGATT TTCATCCAAC 
GACTTCCTTG GGGAGGTATT GATTGATTTA TCTAGCACAT CTCACCTCGA TAACACTCCA 
AGGTGGTATC CTCTCAAAGA ACAGACTGAA AGCATTGATC ATGGCAAGTC TCATTCCAGT 
CAGAGCAGCC AGCAGTCCCC AAAGCCATCT GTTATCAAAA GCAGAAGCCA TGGTATCTTC 
CCTGACCCAT CAAAGGACAT GCAGGTTCCC ACCATTGAGA AATCCCATAG TAGTCCTGGT 
AGCTCAAAAT CATCATCAGA AGGCCATCTC CGTTCTCATG GACCATCTCG CAGTCAAAGC 
AAAACCAGCG TCACTCAGAC CCACCTGGAA GATGCAGGGG CTGCCATAGC TGCTGCCGAA 
GCTGCCGTGC AACAACTCCG CATTCAACCA ACAAAGCCTC CCAATCACCG GCCTGCAGAA 
AGCTCTGTGT CCACCGGCTC CTCGGGCAGC AGCTTTGGCA GTGGGTATAG CGTGGACAGT 
GAAGGAAGCA GCAGCACTGC AGGGGAGACT AATCTATTTC CTATTCCGAG GATAGGGAAG 
ATGGGACAGA ATGGACAAGA GCCTGTAAAA CAGCCAGGGG TAGGAGTAGG ACTAGCAGAC 
ACTGAAGCTA AAACTCAGGT AATGGGAGAA ATCAAGATTG CATTGAAGAA GGAAATGAAG 
ACAGATGGTG AACAACTAAT AGTTGAAATT CTCCAATGCA GAAATATTAC ATACAAATTT 
AAGTCTCCTG ATCATCTACC AGATTTATAT GTGAAAATAT ATGTGATGAA TATTTCTACC 
CAAAAAAAGG TGATCAAGAA AAAAACAAGA GTATGCAGAC ATGATCGAGA GCCTTCGTTT 
AATGAAACTT TTCGATTCAG TCTAAGTCCT GCAGGACATT CTCTTCAGAT TTTACTTTTC 
TCCAATGGAG GGAAGTTTAT GAAAAAGACC TTGATTGGTG AAGCCTGTAT CTGGCTTGAC 
AAAGTGGATC TCAGAAAAAG AATAGTCAAC TGGCACAAAC TTTTGGTCAG TCCTACTCAA 
ACGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCLO ( XM_017012006.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867495.1 Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant X2, mRNA.

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CloneID	OHu97174
Clone ID Related Accession (Same CDS sequence)	XM_017012006.1 , XM_017012006.2
Accession Version	XM_017012006.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein piccolo isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA 
GCGGCTGGAG GAGGAGCTAG CGGGGCGGGG AGCCCCTCTC ACACCGCGAT CCCGGCCGGC 
ATGGAGGCGG ATTTGAGCCA GCTGAGCGAA GAGGAGAGGA GACAGATCGC CGCTGTCATG 
TCAAGGGCGC AGGGGCTGCC CAAGGGAAGC GTCCCCCCGG CCGCTGCGGA GTCGCCTTCC 
ATGCACAGGA AACAAGAGTT GGATAGTAGT CATCCTCCAA AGCAATCAGG AAGACCCCCG 
GACCCTGGGC GTCCAGCTCA ACCTGGTCTC AGTAAAAGTA GAACTACAGA CACTTTCAGG 
TCAGAGCAGA AATTGCCTGG GAGGAGTCCT TCCACTATTA GCTTGAAAGA ATCAAAGTCC 
AGAACTGATT TAAAGGAAGA GCACAAGTCT AGTATGATGC CTGGCTTCCT CTCAGAGGTT 
AACGCTTTAA GTGCTGTTTC CTCTGTTGTA AATAAATTCA ACCCTTTTGA TTTGATATCA 
GACTCTGAGG CATCCCAGGA AGAAACCACC AAGAAACAAA AAGTGGTTCA GAAGGAGCAA 
GGAAAACCTG AAGGAATCAT AAAACCTCCT TTACAACAAC AGCCACCCAA GCCGATTCCT 
AAGCAGCAAG GACCTGGTAG GGATCCGCTT CAGCAGGATG GCACTCCCAA ATCAATATCT 
TCTCAACAAC CAGAAAAAAT TAAATCACAA CCTCCAGGTA CAGGAAAGCC AATTCAGGGT 
CCTACCCAGA CTCCTCAGAC AGACCATGCA AAATTGCCAC TTCAACGAGA TGCATCCAGG 
CCTCAGACTA AACAGGCAGA CATAGTAAGG GGAGAATCAG TTAAACCCTC ACTGCCAAGC 
CCATCCAAAC CACCTATTCA GCAACCAACT CCTGGAAAAC CTCCAGCACA GCAGCCTGGA 
CATGAAAAAT CACAGCCTGG GCCTGCAAAG CCCCCAGCTC AGCCCTCAGG GCTAACAAAG 
CCATTGGCTC AACAACCAGG GACAGTGAAA CCCCCAGTCC AGCCACCAGG GACAACAAAG 
CCTCCAGCTC AGCCTCTTGG TCCTGCTAAG CCTCCAGCTC AGCAGACTGG GTCAGAGAAG 
CCTTCATCGG AGCAGCCTGG GCCAAAGGCT TTAGCTCAGC CTCCTGGAGT TGGAAAGACT 
CCAGCTCAAC AGCCAGGGCC AGCAAAGCCT CCAACCCAGC AGGTGGGGAC ACCAAAACCC 
CTAGCTCAAC AACCTGGGCT ACAGTCTCCA GCTAAGGCAC CTGGGCCTAC AAAGACTCCA 
GTTCAGCAGC CTGGGCCAGG AAAGATTCCA GCTCAACAGG CAGGACCCGG AAAGACTTCT 
GCCCAGCAGA CTGGCCCAAC AAAGCCTCCT TCACAACTGC CTGGCCCAGC AAAGCCCCCA 
CCTCAACAGC CTGGCCCAGC AAAGCCCCCA CCTCAACAGC CTGGCTCAGC AAAGCCCCCA 
TCTCAACAGC CTGGCTCAAC AAAACCCCCA CCTCAACAGC CTGGCCCAGC AAAGCCCTCA 
CCTCAACAGC CTGGCTCAAC AAAACCCCCA TCTCAACAGC CTGGCTCAGC AAAACCCTCA 
GCTCAACAGC CTAGCCCAGC AAAGCCCTCG GCTCAGCAAT CTACAAAACC AGTAAGCCAA 
ACAGGATCTG GAAAACCTCT GCAGCCACCA ACAGTGTCTC CATCTGCAAA ACAGCCTCCT 
TCACAAGGCC TCCCTAAAAC CATCTGTCCT CTTTGCAATA CCACTGAACT TCTGTTGCAT 
GTTCCAGAAA AGGCCAATTT TAACACATGC ACTGAGTGTC AAACCACTGT CTGTAGTCTC 
TGTGGTTTTA ATCCCAATCC TCATTTAACG GAGGTAAAAG AGTGGCTCTG TTTGAACTGT 
CAAATGAAAA GAGCTCTAGG CGGGGATCTG GCTCCAGTTC CGTCATCACC CCAGCCCAAA 
CTGAAGACTG CACCTGTTAC CACTACATCA GCAGTGAGCA AATCATCCCC ACAGCCACAG 
CAGACTTCCC CAAAGAAGGA TGCTGCACCA AAACAGGATC TCTCCAAGGC ACCTGAGCCT 
AAAAAGCCAC CACCACTAGT GAAACAACCA ACCCTTCATG GCTCTCCTTC AGCCAAGGCC 
AAGCAGCCCC CTGAGGCAGA TTCTTTGTCC AAGCCAGCCC CTCCCAAAGA ACCTTCTGTC 
CCATCTGAGC AGGACAAGGC CCCTGTTGCT GATGATAAAC CAAAGCAGCC CAAGATGGTA 
AAGCCAACCA CTGACCTTGT ATCTTCATCA TCAGCAACAA CAAAACCTGA TATTCCAAGC 
TCCAAAGTAC AGTCACAAGC TGAAGAGAAA ACAACCCCTC CTCTAAAAAC AGACTCTGCC 
AAACCCTCAC AGAGTTTTCC ACCAACAGGG GAAAAAGTCA GCCCATTTGA TTCTAAAGCC 
ATACCTCGAC CTGCATCAGA TTCAAAAATT ATTTCACATC CTGGTCCCAG TTCAGAGAGC 
AAAGGTCAAA AACAAGTTGA CCCCGTACAA AAGAAGGAAG AACCCAAGAA AGCACAAACC 
AAAATGAGTC CTAAACCAGA TGCCAAGCCA ATGCCAAAAG GGTCACCAAC ACCCCCTGGC 
CCACGACCTA CCGCTGGCCA AACTGTCCCC ACACCTCAAC AGTCCCCAAA GCCTCAGGAG 
CAGTCAAGGC GTTTCAGTCT GAATCTGGGA AGTATTACTG ATGCCCCCAA ATCACAGCCT 
ACAACTCCTC AAGAGACCGT GACTGGGAAA CTCTTTGGGT TTGGAGCATC AATCTTCAGC 
CAGGCATCAA ATTTAATTTC CACTGCAGGC CAACCTGGAC CTCATTCACA AAGTGGACCA 
GGGGCCCCAA TGAAACAAGC CCCTGCCCCT TCACAGCCAC CTACTTCACA AGGGCCACCC 
AAATCCACAG GTCAAGCACC ACCAGCACCT GCAAAAAGTA TACCTGTGAA AAAGGAAACA 
AAAGCCCCAG CAGCTGAAAA ATTAGAGCCC AAAGCTGAAC AAGCTCCAAC AGTAAAAAGA 
ACAGAAACAG AAAAAAAGCC ACCACCTATT AAGGATAGCA AATCTTTAAC AGCTGAGCCT 
CAAAAGGCTG TCCTTCCCAC AAAACTGGAG AAATCGCCCA AACCAGAATC AACCTGTCCT 
CTCTGCAAAA CTGAACTCAA CATAGGTTCT AAGGATCCTC CTAACTTCAA TACTTGCACT 
GAATGCAAGA ATCAAGTGTG TAATCTCTGT GGATTTAACC CTACACCACA TTTGACTGAG 
ATTCAAGAAT GGCTTTGTTT AAATTGCCAA ACCCAGAGAG CAATATCAGG ACAGCTTGGA 
GACATACGCA AAATGCCACC TGCACCATCA GGACCCAAAG CATCTCCTAT GCCTGTTCCT 
ACAGAATCAT CATCTCAGAA AACAGCAGTG CCTCCCCAAG TAAAATTAGT GAAAAAGCAA 
GAACAAGAAG TAAAAACGGA AGCTGAAAAA GTCATTCTGG AAAAAGTAAA GGAAACACTA 
TCAATGGAAA AAATTCCTCC TATGGTAACC ACAGATCAAA AACAAGAAGA GAGTAAACTA 
GAGAAAGACA AAGCTTCAGC TCTTCAAGAA AAAAAGCCAC TCCCTGAAGA AAAAAAACTA 
ATCCCTGAAG AAGAAAAGAT ACGTTCTGAA GAAAAAAAGC CACTCCTAGA AGAAAAAAAG 
CCAACCCCTG AAGACAAAAA GCTACTCCCA GAGGCAAAAA CATCAGCCCC AGAAGAACAG 
AAACATGACT TACTTAAATC TCAAGTACAA ATTGCTGAAG AAAAGCTTGA AGGCAGAGTG 
GCTCCAAAGA CAGTGCAAGA AGGGAAACAA CCACAGACCA AGATGGAAGG TTTACCATCT 
GGCACACCTC AGAGTTTACC TAAAGAAGAT GATAAGACAA CCAAAACAAT AAAAGAACAG 
CCACAGCCAC CATGCACAGC AAAACCTGAT CAGGTGGAAC CTGGGAAAGA AAAAACAACT 
AAAGGTTCTC AAACCATGAC ATCCTCTGGA GCCCAGAAAA AAGTTAAAAG AACTCTGCCA 
AATCCACCTC CTGAGGAGAT TTCCACAGGA ACTCAATCCA CATTCAGCAC AATGGGCACA 
GTTTCCAGGA GAAGGATCTG CAGAACCAAC ACAATGGCAC GAGCCAAGAT TCTCCAGGAC 
ATAGACAGAG AGCTTGATCT TGTGGAAAGG GAGTCTGCAA AACTTCGAAA GAAACAAGCA 
GAGCTTGATG AAGAAGAAAA GGAGATTGAT GCTAAGCTAC GATACCTGGA AATGGGAATT 
AACAGGAGGA AAGAGGCCCT ATTAAAGGAG AGAGAAAAGA GAGAACGAGC CTACCTCCAG 
GGAGTAGCTG AGGATCGTGA TTACATGTCT GACAGTGAAG TGAGTAGCAC AAGACCAACC 
CGAATAGAAA GTCAGCATGG CATTGAGCGA CCAAGAACTG CTCCCCAAAC TGAATTCAGC 
CAGTTTATAC CACCACAAAC CCAAACAGAA TCTCAACTAG TTCCTCCGAC AAGTCCTTAC 
ACACAATACC AGTACTCTTC CCCTGCTCTT CCTACCCAAG CACCCACCTC ATACACTCAA 
CAGTCTCATT TTGAGCAACA AACTTTGTAC CATCAGCAAG TTTCACCTTA TCAGACTCAG 
CCAACATTCC AAGCTGTGGC AACAATGTCC TTCACACCTC AAGTTCAACC TACACCAACC 
CCACAGCCTT CTTATCAGTT ACCTTCACAG ATGATGGTGA TACAACAGAA GCCACGGCAA 
ACTACATTAT ATTTGGAGCC CAAGATAACC TCAAACTATG AAGTGATTCG CAACCAACCC 
CTTATGATAG CACCTGTTTC TACGGATAAC ACATTTGCTG TTTCCCATCT TGGTAGTAAG 
TACAATAGTT TAGACTTGAG AATAGGTTTG GAGGAAAGAA GTAGCATGGC AAGCAGTCCA 
ATATCAAGCA TATCTGCAGA TTCTTTCTAT GCAGATATTG ATCACCATAC TCCACGAAAT 
TATGTCCTAA TTGACGACAT TGGAGAGATC ACCAAAGGAA CAGCGGCATT AAGCACCGCA 
TTTAGCCTTC ATGAAAAGGA TCTGTCAAAA ACAGACCGTC TCCTTCGAAC CACTGAGACA 
CGCCGGTCTC AAGAAGTGAC AGATTTCCTA GCACCTTTAC AGTCTTCCTC TAGATTGCAT 
AGTTATGTGA AGGCGGAGGA AGACCCAATG GAGGATCCTT ACGAGTTAAA GCTTCTGAAA 
CATCAGATTA AACAGGAATT TCGTAGAGGG ACAGAGAGCT TAGATCACCT TGCTGGTCTT 
TCTCATTATT ACCATGCTGA TACTAGCTAC AGACATTTTC CAAAATCTGA GAAGTATAGC 
ATCAGTAGAC TCACACTTGA AAAACAAGCA GCAAAACAAC TGCCAGCAGC CATACTTTAT 
CAAAAGCAGT CAAAGCATAA GAAATCACTA ATTGACCCTA AAATGTCAAA ATTTTCACCT 
ATTCAAGAAA GTAGAGACCT TGAACCTGAT TATTCAAGCT ATATGACTTC TAGCACTTCA 
TCTATTGGTG GCATTTCCTC CAGGGCAAGG CTCCTTCAAG ATGACATCAC TTTTGGCCTC 
AGAAAAAATA TTACAGACCA ACAAAAATTT ATGGGATCTT CTCTTGGCAC AGGACTGGGC 
ACATTAGGAA ATACCATACG CTCAGCTCTG CAGGATGAAG CGGATAAGCC ATACAGTAGT 
GGCAGCAGGT CCAGACCTTC CTCCAGACCT TCCTCTGTCT ATGGGCTTGA TTTATCAATT 
AAAAGGGATT CTTCTAGCTC TTCCCTAAGA CTGAAAGCTC AAGAGGCTGA AGCTCTAGAT 
GTTTCCTTTA GTCATGCATC ATCCTCTGCC AGAACTAAGC CGACCAGTTT GCCAATTAGT 
CAAAGTAGAG GAAGAATACC AATTGTGGCC CAGAATTCTG AAGAAGAAAG CCCACTCAGT 
CCTGTTGGCC AGCCAATGGG AATGGCCAGG GCTGCAGCTG GACCCCTGCC ACCAATATCT 
GCAGACACCA GGGATCAGTT TGGATCAAGC CACTCATTGC CTGAAGTTCA GCAACACATG 
AGGGAAGAAT CACGGACTCG AGGCTATGAC CGTGACATAG CATTCATCAT GGATGACTTC 
CAACATGCCA TGTCAGACAG TGAAGCCTAT CATCTGCGTC GTGAGGAAAC AGATTGGTTT 
GATAAACCCA GGGAGTCTCG TTTGGAAAAT GGACATGGTC TGGACCGAAA ACTGCCGGAA 
AGATTGGTCC ACTCTAGACC ACTCAGTCAA CATCAAGAGC AAATTATACA GATGAACGGG 
AAAACTATGC ACTACATCTT TCCTCACGCA AGGATAAAAA TAACAAGAGA CTCAAAGGAT 
CACACAGTTT CAGGTAATGG ATTAGGAATT AGAATTGTGG GTGGTAAAGA AATCCCGGGA 
CATAGTGGAG AAATTGGAGC CTATATTGCC AAGATTCTTC CTGGGGGAAG TGCGGAACAG 
ACGGGGAAGC TTATGGAAGG GATGCAAGTA TTGGAATGGA ATGGAATTCC CTTGACTTCT 
AAAACATATG AAGAAGTTCA GAGTATCATT AGTCAGCAAA GTGGGGAAGC AGAAATATGT 
GTAAGACTGG ACCTCAATAT GCTATCAGAT TCTGAAAATT CCCAGCATCT GGAACTTCAT 
GAGCCACCAA AAGCTGTGGA TAAGGCGAAA TCCCCAGGGG TTGATCCTAA GCAGTTGGCA 
GCAGAACTCC AGAAGGTTTC ACTACAGCAG TCACCGCTGG TTCTGTCATC AGTTGTTGAA 
AAAGGATCTC ATGTTCATTC AGGTCCTACA TCAGCAGGAT CCAGTTCCGT TCCCAGCCCT 
GGGCAACCAG GGTCCCCCTC AGTGAGCAAA AAGAAGCACG GCAGCAGCAA GCCTACCGAT 
GGAACAAAGG TTGTCTCTCA TCCAATTACA GGAGAAATTC AGCTTCAAAT TAACTATGAT 
CTTGGAAATC TCATAATACA TATTCTCCAA GCAAGAAATC TTGTTCCTCG AGACAACAAT 
GGTTATTCTG ACCCTTTTGT GAAAGTGTAC CTTCTTCCAG GGAGAGGTCA AGTCATGGTT 
GTCCAGAATG CAAGTGCTGA GTACAAGAGA AGGACTAAAC ATGTCCAGAA AAGTCTTAAT 
CCTGAGTGGA ATCAAACAGT AATTTATAAA AGTATTTCCA TGGAACAGCT CAAGAAGAAA 
ACACTGGAGG TGACAGTTTG GGATTATGAT AGATTTTCAT CCAACGACTT CCTTGGGGAG 
GTATTGATTG ATTTATCTAG CACATCTCAC CTCGATAACA CTCCAAGGTG GTATCCTCTC 
AAAGAACAGA CTGAAAGCAT TGATCATGGC AAGTCTCATT CCAGTCAGAG CAGCCAGCAG 
TCCCCAAAGC CATCTGTTAT CAAAAGCAGA AGCCATGGTA TCTTCCCTGA CCCATCAAAG 
GACATGCAGG TTCCCACCAT TGAGAAATCC CATAGTAGTC CTGGTAGCTC AAAATCATCA 
TCAGAAGGCC ATCTCCGTTC TCATGGACCA TCTCGCAGTC AAAGCAAAAC CAGCGTCACT 
CAGACCCACC TGGAAGATGC AGGGGCTGCC ATAGCTGCTG CCGAAGCTGC CGTGCAACAA 
CTCCGCATTC AACCAACAGC ACATAAAAGC GGTCAGTCTA ATCATGCCAG GAAGCAGCAC 
AGACACAGCA TAGCAGGAGT CCTCCCCATT CAAAGAACTC AGTCTGATAA TCTGCCTCCA 
CCAGCCAATG GCAACCAAGA CCAAAGCCAG CTAGCCCTCA GGAAGGTGAT GTCTGATGGA 
CCAGTCAAGC CAGAAGGAGC AAAGCCTCCC AATCACCGGC CTGCAGAAAG CTCTGTGTCC 
ACCGGCTCCT CGGGCAGCAG CTTTGGCAGT GGGTATAGCG TGGACAGTGA AGGAAGCAGC 
AGCACTGCAG GGGAGACTAA TCTATTTCCT ATTCCGAGGA TAGGGAAGAT GGGACAGAAT 
GGACAAGAGC CTGTAAAACA GCCAGGGGTA GGAGTAGGAC TAGCAGACAC TGAAGCTAAA 
ACTCAGGTAA TGGGAGAAAT CAAGATTGCA TTGAAGAAGG AAATGAAGAC AGATGGTGAA 
CAACTAATAG TTGAAATTCT CCAATGCAGA AATATTACAT ACAAATTTAA GTCTCCTGAT 
CATCTACCAG ATTTATATGT GAAAATATAT GTGATGAATA TTTCTACCCA AAAAAAGGTG 
ATCAAGAAAA AAACAAGAGT ATGCAGACAT GATCGAGAGC CTTCGTTTAA TGAAACTTTT 
CGATTCAGTC TAAGTCCTGC AGGACATTCT CTTCAGATTT TACTTTTCTC CAATGGAGGG 
AAGTTTATGA AAAAGACCTT GATTGGTGAA GCCTGTATCT GGCTTGACAA AGTGGATCTC 
AGAAAAAGAA TAGTCAACTG GCACAAACTT TTGGTCAGTC CTACTCAAAC GCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867495.1
CDS	1565..10081
Translation	MGNEASLEGEGLPEGLAAAAAAGGGASGAGSPSHTAIPAGMEADLSQLSEEERRQIAAVMSRAQGLPKGSVPPAAAESPSMHRKQELDSSHPPKQSGRPPDPGRPAQPGLSKSRTTDTFRSEQKLPGRSPSTISLKESKSRTDLKEEHKSSMMPGFLSEVNALSAVSSVVNKFNPFDLISDSEASQEETTKKQKVVQKEQGKPEGIIKPPLQQQPPKPIPKQQGPGRDPLQQDGTPKSISSQQPEKIKSQPPGTGKPIQGPTQTPQTDHAKLPLQRDASRPQTKQADIVRGESVKPSLPSPSKPPIQQPTPGKPPAQQPGHEKSQPGPAKPPAQPSGLTKPLAQQPGTVKPPVQPPGTTKPPAQPLGPAKPPAQQTGSEKPSSEQPGPKALAQPPGVGKTPAQQPGPAKPPTQQVGTPKPLAQQPGLQSPAKAPGPTKTPVQQPGPGKIPAQQAGPGKTSAQQTGPTKPPSQLPGPAKPPPQQPGPAKPPPQQPGSAKPPSQQPGSTKPPPQQPGPAKPSPQQPGSTKPPSQQPGSAKPSAQQPSPAKPSAQQSTKPVSQTGSGKPLQPPTVSPSAKQPPSQGLPKTICPLCNTTELLLHVPEKANFNTCTECQTTVCSLCGFNPNPHLTEVKEWLCLNCQMKRALGGDLAPVPSSPQPKLKTAPVTTTSAVSKSSPQPQQTSPKKDAAPKQDLSKAPEPKKPPPLVKQPTLHGSPSAKAKQPPEADSLSKPAPPKEPSVPSEQDKAPVADDKPKQPKMVKPTTDLVSSSSATTKPDIPSSKVQSQAEEKTTPPLKTDSAKPSQSFPPTGEKVSPFDSKAIPRPASDSKIISHPGPSSESKGQKQVDPVQKKEEPKKAQTKMSPKPDAKPMPKGSPTPPGPRPTAGQTVPTPQQSPKPQEQSRRFSLNLGSITDAPKSQPTTPQETVTGKLFGFGASIFSQASNLISTAGQPGPHSQSGPGAPMKQAPAPSQPPTSQGPPKSTGQAPPAPAKSIPVKKETKAPAAEKLEPKAEQAPTVKRTETEKKPPPIKDSKSLTAEPQKAVLPTKLEKSPKPESTCPLCKTELNIGSKDPPNFNTCTECKNQVCNLCGFNPTPHLTEIQEWLCLNCQTQRAISGQLGDIRKMPPAPSGPKASPMPVPTESSSQKTAVPPQVKLVKKQEQEVKTEAEKVILEKVKETLSMEKIPPMVTTDQKQEESKLEKDKASALQEKKPLPEEKKLIPEEEKIRSEEKKPLLEEKKPTPEDKKLLPEAKTSAPEEQKHDLLKSQVQIAEEKLEGRVAPKTVQEGKQPQTKMEGLPSGTPQSLPKEDDKTTKTIKEQPQPPCTAKPDQVEPGKEKTTKGSQTMTSSGAQKKVKRTLPNPPPEEISTGTQSTFSTMGTVSRRRICRTNTMARAKILQDIDRELDLVERESAKLRKKQAELDEEEKEIDAKLRYLEMGINRRKEALLKEREKRERAYLQGVAEDRDYMSDSEVSSTRPTRIESQHGIERPRTAPQTEFSQFIPPQTQTESQLVPPTSPYTQYQYSSPALPTQAPTSYTQQSHFEQQTLYHQQVSPYQTQPTFQAVATMSFTPQVQPTPTPQPSYQLPSQMMVIQQKPRQTTLYLEPKITSNYEVIRNQPLMIAPVSTDNTFAVSHLGSKYNSLDLRIGLEERSSMASSPISSISADSFYADIDHHTPRNYVLIDDIGEITKGTAALSTAFSLHEKDLSKTDRLLRTTETRRSQEVTDFLAPLQSSSRLHSYVKAEEDPMEDPYELKLLKHQIKQEFRRGTESLDHLAGLSHYYHADTSYRHFPKSEKYSISRLTLEKQAAKQLPAAILYQKQSKHKKSLIDPKMSKFSPIQESRDLEPDYSSYMTSSTSSIGGISSRARLLQDDITFGLRKNITDQQKFMGSSLGTGLGTLGNTIRSALQDEADKPYSSGSRSRPSSRPSSVYGLDLSIKRDSSSSSLRLKAQEAEALDVSFSHASSSARTKPTSLPISQSRGRIPIVAQNSEEESPLSPVGQPMGMARAAAGPLPPISADTRDQFGSSHSLPEVQQHMREESRTRGYDRDIAFIMDDFQHAMSDSEAYHLRREETDWFDKPRESRLENGHGLDRKLPERLVHSRPLSQHQEQIIQMNGKTMHYIFPHARIKITRDSKDHTVSGNGLGIRIVGGKEIPGHSGEIGAYIAKILPGGSAEQTGKLMEGMQVLEWNGIPLTSKTYEEVQSIISQQSGEAEICVRLDLNMLSDSENSQHLELHEPPKAVDKAKSPGVDPKQLAAELQKVSLQQSPLVLSSVVEKGSHVHSGPTSAGSSSVPSPGQPGSPSVSKKKHGSSKPTDGTKVVSHPITGEIQLQINYDLGNLIIHILQARNLVPRDNNGYSDPFVKVYLLPGRGQVMVVQNASAEYKRRTKHVQKSLNPEWNQTVIYKSISMEQLKKKTLEVTVWDYDRFSSNDFLGEVLIDLSSTSHLDNTPRWYPLKEQTESIDHGKSHSSQSSQQSPKPSVIKSRSHGIFPDPSKDMQVPTIEKSHSSPGSSKSSSEGHLRSHGPSRSQSKTSVTQTHLEDAGAAIAAAEAAVQQLRIQPTAHKSGQSNHARKQHRHSIAGVLPIQRTQSDNLPPPANGNQDQSQLALRKVMSDGPVKPEGAKPPNHRPAESSVSTGSSGSSFGSGYSVDSEGSSSTAGETNLFPIPRIGKMGQNGQEPVKQPGVGVGLADTEAKTQVMGEIKIALKKEMKTDGEQLIVEILQCRNITYKFKSPDHLPDLYVKIYVMNISTQKKVIKKKTRVCRHDREPSFNETFRFSLSPAGHSLQILLFSNGGKFMKKTLIGEACIWLDKVDLRKRIVNWHKLLVSPTQTH

Target ORF information:

RefSeq Version	XM_017012006.1
Organism	Homo sapiens(human)
Definition	Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012006.1

ORF Insert Sequence:

ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA 
GCGGCTGGAG GAGGAGCTAG CGGGGCGGGG AGCCCCTCTC ACACCGCGAT CCCGGCCGGC 
ATGGAGGCGG ATTTGAGCCA GCTGAGCGAA GAGGAGAGGA GACAGATCGC CGCTGTCATG 
TCAAGGGCGC AGGGGCTGCC CAAGGGAAGC GTCCCCCCGG CCGCTGCGGA GTCGCCTTCC 
ATGCACAGGA AACAAGAGTT GGATAGTAGT CATCCTCCAA AGCAATCAGG AAGACCCCCG 
GACCCTGGGC GTCCAGCTCA ACCTGGTCTC AGTAAAAGTA GAACTACAGA CACTTTCAGG 
TCAGAGCAGA AATTGCCTGG GAGGAGTCCT TCCACTATTA GCTTGAAAGA ATCAAAGTCC 
AGAACTGATT TAAAGGAAGA GCACAAGTCT AGTATGATGC CTGGCTTCCT CTCAGAGGTT 
AACGCTTTAA GTGCTGTTTC CTCTGTTGTA AATAAATTCA ACCCTTTTGA TTTGATATCA 
GACTCTGAGG CATCCCAGGA AGAAACCACC AAGAAACAAA AAGTGGTTCA GAAGGAGCAA 
GGAAAACCTG AAGGAATCAT AAAACCTCCT TTACAACAAC AGCCACCCAA GCCGATTCCT 
AAGCAGCAAG GACCTGGTAG GGATCCGCTT CAGCAGGATG GCACTCCCAA ATCAATATCT 
TCTCAACAAC CAGAAAAAAT TAAATCACAA CCTCCAGGTA CAGGAAAGCC AATTCAGGGT 
CCTACCCAGA CTCCTCAGAC AGACCATGCA AAATTGCCAC TTCAACGAGA TGCATCCAGG 
CCTCAGACTA AACAGGCAGA CATAGTAAGG GGAGAATCAG TTAAACCCTC ACTGCCAAGC 
CCATCCAAAC CACCTATTCA GCAACCAACT CCTGGAAAAC CTCCAGCACA GCAGCCTGGA 
CATGAAAAAT CACAGCCTGG GCCTGCAAAG CCCCCAGCTC AGCCCTCAGG GCTAACAAAG 
CCATTGGCTC AACAACCAGG GACAGTGAAA CCCCCAGTCC AGCCACCAGG GACAACAAAG 
CCTCCAGCTC AGCCTCTTGG TCCTGCTAAG CCTCCAGCTC AGCAGACTGG GTCAGAGAAG 
CCTTCATCGG AGCAGCCTGG GCCAAAGGCT TTAGCTCAGC CTCCTGGAGT TGGAAAGACT 
CCAGCTCAAC AGCCAGGGCC AGCAAAGCCT CCAACCCAGC AGGTGGGGAC ACCAAAACCC 
CTAGCTCAAC AACCTGGGCT ACAGTCTCCA GCTAAGGCAC CTGGGCCTAC AAAGACTCCA 
GTTCAGCAGC CTGGGCCAGG AAAGATTCCA GCTCAACAGG CAGGACCCGG AAAGACTTCT 
GCCCAGCAGA CTGGCCCAAC AAAGCCTCCT TCACAACTGC CTGGCCCAGC AAAGCCCCCA 
CCTCAACAGC CTGGCCCAGC AAAGCCCCCA CCTCAACAGC CTGGCTCAGC AAAGCCCCCA 
TCTCAACAGC CTGGCTCAAC AAAACCCCCA CCTCAACAGC CTGGCCCAGC AAAGCCCTCA 
CCTCAACAGC CTGGCTCAAC AAAACCCCCA TCTCAACAGC CTGGCTCAGC AAAACCCTCA 
GCTCAACAGC CTAGCCCAGC AAAGCCCTCG GCTCAGCAAT CTACAAAACC AGTAAGCCAA 
ACAGGATCTG GAAAACCTCT GCAGCCACCA ACAGTGTCTC CATCTGCAAA ACAGCCTCCT 
TCACAAGGCC TCCCTAAAAC CATCTGTCCT CTTTGCAATA CCACTGAACT TCTGTTGCAT 
GTTCCAGAAA AGGCCAATTT TAACACATGC ACTGAGTGTC AAACCACTGT CTGTAGTCTC 
TGTGGTTTTA ATCCCAATCC TCATTTAACG GAGGTAAAAG AGTGGCTCTG TTTGAACTGT 
CAAATGAAAA GAGCTCTAGG CGGGGATCTG GCTCCAGTTC CGTCATCACC CCAGCCCAAA 
CTGAAGACTG CACCTGTTAC CACTACATCA GCAGTGAGCA AATCATCCCC ACAGCCACAG 
CAGACTTCCC CAAAGAAGGA TGCTGCACCA AAACAGGATC TCTCCAAGGC ACCTGAGCCT 
AAAAAGCCAC CACCACTAGT GAAACAACCA ACCCTTCATG GCTCTCCTTC AGCCAAGGCC 
AAGCAGCCCC CTGAGGCAGA TTCTTTGTCC AAGCCAGCCC CTCCCAAAGA ACCTTCTGTC 
CCATCTGAGC AGGACAAGGC CCCTGTTGCT GATGATAAAC CAAAGCAGCC CAAGATGGTA 
AAGCCAACCA CTGACCTTGT ATCTTCATCA TCAGCAACAA CAAAACCTGA TATTCCAAGC 
TCCAAAGTAC AGTCACAAGC TGAAGAGAAA ACAACCCCTC CTCTAAAAAC AGACTCTGCC 
AAACCCTCAC AGAGTTTTCC ACCAACAGGG GAAAAAGTCA GCCCATTTGA TTCTAAAGCC 
ATACCTCGAC CTGCATCAGA TTCAAAAATT ATTTCACATC CTGGTCCCAG TTCAGAGAGC 
AAAGGTCAAA AACAAGTTGA CCCCGTACAA AAGAAGGAAG AACCCAAGAA AGCACAAACC 
AAAATGAGTC CTAAACCAGA TGCCAAGCCA ATGCCAAAAG GGTCACCAAC ACCCCCTGGC 
CCACGACCTA CCGCTGGCCA AACTGTCCCC ACACCTCAAC AGTCCCCAAA GCCTCAGGAG 
CAGTCAAGGC GTTTCAGTCT GAATCTGGGA AGTATTACTG ATGCCCCCAA ATCACAGCCT 
ACAACTCCTC AAGAGACCGT GACTGGGAAA CTCTTTGGGT TTGGAGCATC AATCTTCAGC 
CAGGCATCAA ATTTAATTTC CACTGCAGGC CAACCTGGAC CTCATTCACA AAGTGGACCA 
GGGGCCCCAA TGAAACAAGC CCCTGCCCCT TCACAGCCAC CTACTTCACA AGGGCCACCC 
AAATCCACAG GTCAAGCACC ACCAGCACCT GCAAAAAGTA TACCTGTGAA AAAGGAAACA 
AAAGCCCCAG CAGCTGAAAA ATTAGAGCCC AAAGCTGAAC AAGCTCCAAC AGTAAAAAGA 
ACAGAAACAG AAAAAAAGCC ACCACCTATT AAGGATAGCA AATCTTTAAC AGCTGAGCCT 
CAAAAGGCTG TCCTTCCCAC AAAACTGGAG AAATCGCCCA AACCAGAATC AACCTGTCCT 
CTCTGCAAAA CTGAACTCAA CATAGGTTCT AAGGATCCTC CTAACTTCAA TACTTGCACT 
GAATGCAAGA ATCAAGTGTG TAATCTCTGT GGATTTAACC CTACACCACA TTTGACTGAG 
ATTCAAGAAT GGCTTTGTTT AAATTGCCAA ACCCAGAGAG CAATATCAGG ACAGCTTGGA 
GACATACGCA AAATGCCACC TGCACCATCA GGACCCAAAG CATCTCCTAT GCCTGTTCCT 
ACAGAATCAT CATCTCAGAA AACAGCAGTG CCTCCCCAAG TAAAATTAGT GAAAAAGCAA 
GAACAAGAAG TAAAAACGGA AGCTGAAAAA GTCATTCTGG AAAAAGTAAA GGAAACACTA 
TCAATGGAAA AAATTCCTCC TATGGTAACC ACAGATCAAA AACAAGAAGA GAGTAAACTA 
GAGAAAGACA AAGCTTCAGC TCTTCAAGAA AAAAAGCCAC TCCCTGAAGA AAAAAAACTA 
ATCCCTGAAG AAGAAAAGAT ACGTTCTGAA GAAAAAAAGC CACTCCTAGA AGAAAAAAAG 
CCAACCCCTG AAGACAAAAA GCTACTCCCA GAGGCAAAAA CATCAGCCCC AGAAGAACAG 
AAACATGACT TACTTAAATC TCAAGTACAA ATTGCTGAAG AAAAGCTTGA AGGCAGAGTG 
GCTCCAAAGA CAGTGCAAGA AGGGAAACAA CCACAGACCA AGATGGAAGG TTTACCATCT 
GGCACACCTC AGAGTTTACC TAAAGAAGAT GATAAGACAA CCAAAACAAT AAAAGAACAG 
CCACAGCCAC CATGCACAGC AAAACCTGAT CAGGTGGAAC CTGGGAAAGA AAAAACAACT 
AAAGGTTCTC AAACCATGAC ATCCTCTGGA GCCCAGAAAA AAGTTAAAAG AACTCTGCCA 
AATCCACCTC CTGAGGAGAT TTCCACAGGA ACTCAATCCA CATTCAGCAC AATGGGCACA 
GTTTCCAGGA GAAGGATCTG CAGAACCAAC ACAATGGCAC GAGCCAAGAT TCTCCAGGAC 
ATAGACAGAG AGCTTGATCT TGTGGAAAGG GAGTCTGCAA AACTTCGAAA GAAACAAGCA 
GAGCTTGATG AAGAAGAAAA GGAGATTGAT GCTAAGCTAC GATACCTGGA AATGGGAATT 
AACAGGAGGA AAGAGGCCCT ATTAAAGGAG AGAGAAAAGA GAGAACGAGC CTACCTCCAG 
GGAGTAGCTG AGGATCGTGA TTACATGTCT GACAGTGAAG TGAGTAGCAC AAGACCAACC 
CGAATAGAAA GTCAGCATGG CATTGAGCGA CCAAGAACTG CTCCCCAAAC TGAATTCAGC 
CAGTTTATAC CACCACAAAC CCAAACAGAA TCTCAACTAG TTCCTCCGAC AAGTCCTTAC 
ACACAATACC AGTACTCTTC CCCTGCTCTT CCTACCCAAG CACCCACCTC ATACACTCAA 
CAGTCTCATT TTGAGCAACA AACTTTGTAC CATCAGCAAG TTTCACCTTA TCAGACTCAG 
CCAACATTCC AAGCTGTGGC AACAATGTCC TTCACACCTC AAGTTCAACC TACACCAACC 
CCACAGCCTT CTTATCAGTT ACCTTCACAG ATGATGGTGA TACAACAGAA GCCACGGCAA 
ACTACATTAT ATTTGGAGCC CAAGATAACC TCAAACTATG AAGTGATTCG CAACCAACCC 
CTTATGATAG CACCTGTTTC TACGGATAAC ACATTTGCTG TTTCCCATCT TGGTAGTAAG 
TACAATAGTT TAGACTTGAG AATAGGTTTG GAGGAAAGAA GTAGCATGGC AAGCAGTCCA 
ATATCAAGCA TATCTGCAGA TTCTTTCTAT GCAGATATTG ATCACCATAC TCCACGAAAT 
TATGTCCTAA TTGACGACAT TGGAGAGATC ACCAAAGGAA CAGCGGCATT AAGCACCGCA 
TTTAGCCTTC ATGAAAAGGA TCTGTCAAAA ACAGACCGTC TCCTTCGAAC CACTGAGACA 
CGCCGGTCTC AAGAAGTGAC AGATTTCCTA GCACCTTTAC AGTCTTCCTC TAGATTGCAT 
AGTTATGTGA AGGCGGAGGA AGACCCAATG GAGGATCCTT ACGAGTTAAA GCTTCTGAAA 
CATCAGATTA AACAGGAATT TCGTAGAGGG ACAGAGAGCT TAGATCACCT TGCTGGTCTT 
TCTCATTATT ACCATGCTGA TACTAGCTAC AGACATTTTC CAAAATCTGA GAAGTATAGC 
ATCAGTAGAC TCACACTTGA AAAACAAGCA GCAAAACAAC TGCCAGCAGC CATACTTTAT 
CAAAAGCAGT CAAAGCATAA GAAATCACTA ATTGACCCTA AAATGTCAAA ATTTTCACCT 
ATTCAAGAAA GTAGAGACCT TGAACCTGAT TATTCAAGCT ATATGACTTC TAGCACTTCA 
TCTATTGGTG GCATTTCCTC CAGGGCAAGG CTCCTTCAAG ATGACATCAC TTTTGGCCTC 
AGAAAAAATA TTACAGACCA ACAAAAATTT ATGGGATCTT CTCTTGGCAC AGGACTGGGC 
ACATTAGGAA ATACCATACG CTCAGCTCTG CAGGATGAAG CGGATAAGCC ATACAGTAGT 
GGCAGCAGGT CCAGACCTTC CTCCAGACCT TCCTCTGTCT ATGGGCTTGA TTTATCAATT 
AAAAGGGATT CTTCTAGCTC TTCCCTAAGA CTGAAAGCTC AAGAGGCTGA AGCTCTAGAT 
GTTTCCTTTA GTCATGCATC ATCCTCTGCC AGAACTAAGC CGACCAGTTT GCCAATTAGT 
CAAAGTAGAG GAAGAATACC AATTGTGGCC CAGAATTCTG AAGAAGAAAG CCCACTCAGT 
CCTGTTGGCC AGCCAATGGG AATGGCCAGG GCTGCAGCTG GACCCCTGCC ACCAATATCT 
GCAGACACCA GGGATCAGTT TGGATCAAGC CACTCATTGC CTGAAGTTCA GCAACACATG 
AGGGAAGAAT CACGGACTCG AGGCTATGAC CGTGACATAG CATTCATCAT GGATGACTTC 
CAACATGCCA TGTCAGACAG TGAAGCCTAT CATCTGCGTC GTGAGGAAAC AGATTGGTTT 
GATAAACCCA GGGAGTCTCG TTTGGAAAAT GGACATGGTC TGGACCGAAA ACTGCCGGAA 
AGATTGGTCC ACTCTAGACC ACTCAGTCAA CATCAAGAGC AAATTATACA GATGAACGGG 
AAAACTATGC ACTACATCTT TCCTCACGCA AGGATAAAAA TAACAAGAGA CTCAAAGGAT 
CACACAGTTT CAGGTAATGG ATTAGGAATT AGAATTGTGG GTGGTAAAGA AATCCCGGGA 
CATAGTGGAG AAATTGGAGC CTATATTGCC AAGATTCTTC CTGGGGGAAG TGCGGAACAG 
ACGGGGAAGC TTATGGAAGG GATGCAAGTA TTGGAATGGA ATGGAATTCC CTTGACTTCT 
AAAACATATG AAGAAGTTCA GAGTATCATT AGTCAGCAAA GTGGGGAAGC AGAAATATGT 
GTAAGACTGG ACCTCAATAT GCTATCAGAT TCTGAAAATT CCCAGCATCT GGAACTTCAT 
GAGCCACCAA AAGCTGTGGA TAAGGCGAAA TCCCCAGGGG TTGATCCTAA GCAGTTGGCA 
GCAGAACTCC AGAAGGTTTC ACTACAGCAG TCACCGCTGG TTCTGTCATC AGTTGTTGAA 
AAAGGATCTC ATGTTCATTC AGGTCCTACA TCAGCAGGAT CCAGTTCCGT TCCCAGCCCT 
GGGCAACCAG GGTCCCCCTC AGTGAGCAAA AAGAAGCACG GCAGCAGCAA GCCTACCGAT 
GGAACAAAGG TTGTCTCTCA TCCAATTACA GGAGAAATTC AGCTTCAAAT TAACTATGAT 
CTTGGAAATC TCATAATACA TATTCTCCAA GCAAGAAATC TTGTTCCTCG AGACAACAAT 
GGTTATTCTG ACCCTTTTGT GAAAGTGTAC CTTCTTCCAG GGAGAGGTCA AGTCATGGTT 
GTCCAGAATG CAAGTGCTGA GTACAAGAGA AGGACTAAAC ATGTCCAGAA AAGTCTTAAT 
CCTGAGTGGA ATCAAACAGT AATTTATAAA AGTATTTCCA TGGAACAGCT CAAGAAGAAA 
ACACTGGAGG TGACAGTTTG GGATTATGAT AGATTTTCAT CCAACGACTT CCTTGGGGAG 
GTATTGATTG ATTTATCTAG CACATCTCAC CTCGATAACA CTCCAAGGTG GTATCCTCTC 
AAAGAACAGA CTGAAAGCAT TGATCATGGC AAGTCTCATT CCAGTCAGAG CAGCCAGCAG 
TCCCCAAAGC CATCTGTTAT CAAAAGCAGA AGCCATGGTA TCTTCCCTGA CCCATCAAAG 
GACATGCAGG TTCCCACCAT TGAGAAATCC CATAGTAGTC CTGGTAGCTC AAAATCATCA 
TCAGAAGGCC ATCTCCGTTC TCATGGACCA TCTCGCAGTC AAAGCAAAAC CAGCGTCACT 
CAGACCCACC TGGAAGATGC AGGGGCTGCC ATAGCTGCTG CCGAAGCTGC CGTGCAACAA 
CTCCGCATTC AACCAACAGC ACATAAAAGC GGTCAGTCTA ATCATGCCAG GAAGCAGCAC 
AGACACAGCA TAGCAGGAGT CCTCCCCATT CAAAGAACTC AGTCTGATAA TCTGCCTCCA 
CCAGCCAATG GCAACCAAGA CCAAAGCCAG CTAGCCCTCA GGAAGGTGAT GTCTGATGGA 
CCAGTCAAGC CAGAAGGAGC AAAGCCTCCC AATCACCGGC CTGCAGAAAG CTCTGTGTCC 
ACCGGCTCCT CGGGCAGCAG CTTTGGCAGT GGGTATAGCG TGGACAGTGA AGGAAGCAGC 
AGCACTGCAG GGGAGACTAA TCTATTTCCT ATTCCGAGGA TAGGGAAGAT GGGACAGAAT 
GGACAAGAGC CTGTAAAACA GCCAGGGGTA GGAGTAGGAC TAGCAGACAC TGAAGCTAAA 
ACTCAGGTAA TGGGAGAAAT CAAGATTGCA TTGAAGAAGG AAATGAAGAC AGATGGTGAA 
CAACTAATAG TTGAAATTCT CCAATGCAGA AATATTACAT ACAAATTTAA GTCTCCTGAT 
CATCTACCAG ATTTATATGT GAAAATATAT GTGATGAATA TTTCTACCCA AAAAAAGGTG 
ATCAAGAAAA AAACAAGAGT ATGCAGACAT GATCGAGAGC CTTCGTTTAA TGAAACTTTT 
CGATTCAGTC TAAGTCCTGC AGGACATTCT CTTCAGATTT TACTTTTCTC CAATGGAGGG 
AAGTTTATGA AAAAGACCTT GATTGGTGAA GCCTGTATCT GGCTTGACAA AGTGGATCTC 
AGAAAAAGAA TAGTCAACTG GCACAAACTT TTGGTCAGTC CTACTCAAAC GCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCLO ( XM_017012006.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867495.1 Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant X2, mRNA.

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CloneID	OHu97174
Clone ID Related Accession (Same CDS sequence)	XM_017012006.1 , XM_017012006.2
Accession Version	XM_017012006.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein piccolo isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017012006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA 
GCGGCTGGAG GAGGAGCTAG CGGGGCGGGG AGCCCCTCTC ACACCGCGAT CCCGGCCGGC 
ATGGAGGCGG ATTTGAGCCA GCTGAGCGAA GAGGAGAGGA GACAGATCGC CGCTGTCATG 
TCAAGGGCGC AGGGGCTGCC CAAGGGAAGC GTCCCCCCGG CCGCTGCGGA GTCGCCTTCC 
ATGCACAGGA AACAAGAGTT GGATAGTAGT CATCCTCCAA AGCAATCAGG AAGACCCCCG 
GACCCTGGGC GTCCAGCTCA ACCTGGTCTC AGTAAAAGTA GAACTACAGA CACTTTCAGG 
TCAGAGCAGA AATTGCCTGG GAGGAGTCCT TCCACTATTA GCTTGAAAGA ATCAAAGTCC 
AGAACTGATT TAAAGGAAGA GCACAAGTCT AGTATGATGC CTGGCTTCCT CTCAGAGGTT 
AACGCTTTAA GTGCTGTTTC CTCTGTTGTA AATAAATTCA ACCCTTTTGA TTTGATATCA 
GACTCTGAGG CATCCCAGGA AGAAACCACC AAGAAACAAA AAGTGGTTCA GAAGGAGCAA 
GGAAAACCTG AAGGAATCAT AAAACCTCCT TTACAACAAC AGCCACCCAA GCCGATTCCT 
AAGCAGCAAG GACCTGGTAG GGATCCGCTT CAGCAGGATG GCACTCCCAA ATCAATATCT 
TCTCAACAAC CAGAAAAAAT TAAATCACAA CCTCCAGGTA CAGGAAAGCC AATTCAGGGT 
CCTACCCAGA CTCCTCAGAC AGACCATGCA AAATTGCCAC TTCAACGAGA TGCATCCAGG 
CCTCAGACTA AACAGGCAGA CATAGTAAGG GGAGAATCAG TTAAACCCTC ACTGCCAAGC 
CCATCCAAAC CACCTATTCA GCAACCAACT CCTGGAAAAC CTCCAGCACA GCAGCCTGGA 
CATGAAAAAT CACAGCCTGG GCCTGCAAAG CCCCCAGCTC AGCCCTCAGG GCTAACAAAG 
CCATTGGCTC AACAACCAGG GACAGTGAAA CCCCCAGTCC AGCCACCAGG GACAACAAAG 
CCTCCAGCTC AGCCTCTTGG TCCTGCTAAG CCTCCAGCTC AGCAGACTGG GTCAGAGAAG 
CCTTCATCGG AGCAGCCTGG GCCAAAGGCT TTAGCTCAGC CTCCTGGAGT TGGAAAGACT 
CCAGCTCAAC AGCCAGGGCC AGCAAAGCCT CCAACCCAGC AGGTGGGGAC ACCAAAACCC 
CTAGCTCAAC AACCTGGGCT ACAGTCTCCA GCTAAGGCAC CTGGGCCTAC AAAGACTCCA 
GTTCAGCAGC CTGGGCCAGG AAAGATTCCA GCTCAACAGG CAGGACCCGG AAAGACTTCT 
GCCCAGCAGA CTGGCCCAAC AAAGCCTCCT TCACAACTGC CTGGCCCAGC AAAGCCCCCA 
CCTCAACAGC CTGGCCCAGC AAAGCCCCCA CCTCAACAGC CTGGCTCAGC AAAGCCCCCA 
TCTCAACAGC CTGGCTCAAC AAAACCCCCA CCTCAACAGC CTGGCCCAGC AAAGCCCTCA 
CCTCAACAGC CTGGCTCAAC AAAACCCCCA TCTCAACAGC CTGGCTCAGC AAAACCCTCA 
GCTCAACAGC CTAGCCCAGC AAAGCCCTCG GCTCAGCAAT CTACAAAACC AGTAAGCCAA 
ACAGGATCTG GAAAACCTCT GCAGCCACCA ACAGTGTCTC CATCTGCAAA ACAGCCTCCT 
TCACAAGGCC TCCCTAAAAC CATCTGTCCT CTTTGCAATA CCACTGAACT TCTGTTGCAT 
GTTCCAGAAA AGGCCAATTT TAACACATGC ACTGAGTGTC AAACCACTGT CTGTAGTCTC 
TGTGGTTTTA ATCCCAATCC TCATTTAACG GAGGTAAAAG AGTGGCTCTG TTTGAACTGT 
CAAATGAAAA GAGCTCTAGG CGGGGATCTG GCTCCAGTTC CGTCATCACC CCAGCCCAAA 
CTGAAGACTG CACCTGTTAC CACTACATCA GCAGTGAGCA AATCATCCCC ACAGCCACAG 
CAGACTTCCC CAAAGAAGGA TGCTGCACCA AAACAGGATC TCTCCAAGGC ACCTGAGCCT 
AAAAAGCCAC CACCACTAGT GAAACAACCA ACCCTTCATG GCTCTCCTTC AGCCAAGGCC 
AAGCAGCCCC CTGAGGCAGA TTCTTTGTCC AAGCCAGCCC CTCCCAAAGA ACCTTCTGTC 
CCATCTGAGC AGGACAAGGC CCCTGTTGCT GATGATAAAC CAAAGCAGCC CAAGATGGTA 
AAGCCAACCA CTGACCTTGT ATCTTCATCA TCAGCAACAA CAAAACCTGA TATTCCAAGC 
TCCAAAGTAC AGTCACAAGC TGAAGAGAAA ACAACCCCTC CTCTAAAAAC AGACTCTGCC 
AAACCCTCAC AGAGTTTTCC ACCAACAGGG GAAAAAGTCA GCCCATTTGA TTCTAAAGCC 
ATACCTCGAC CTGCATCAGA TTCAAAAATT ATTTCACATC CTGGTCCCAG TTCAGAGAGC 
AAAGGTCAAA AACAAGTTGA CCCCGTACAA AAGAAGGAAG AACCCAAGAA AGCACAAACC 
AAAATGAGTC CTAAACCAGA TGCCAAGCCA ATGCCAAAAG GGTCACCAAC ACCCCCTGGC 
CCACGACCTA CCGCTGGCCA AACTGTCCCC ACACCTCAAC AGTCCCCAAA GCCTCAGGAG 
CAGTCAAGGC GTTTCAGTCT GAATCTGGGA AGTATTACTG ATGCCCCCAA ATCACAGCCT 
ACAACTCCTC AAGAGACCGT GACTGGGAAA CTCTTTGGGT TTGGAGCATC AATCTTCAGC 
CAGGCATCAA ATTTAATTTC CACTGCAGGC CAACCTGGAC CTCATTCACA AAGTGGACCA 
GGGGCCCCAA TGAAACAAGC CCCTGCCCCT TCACAGCCAC CTACTTCACA AGGGCCACCC 
AAATCCACAG GTCAAGCACC ACCAGCACCT GCAAAAAGTA TACCTGTGAA AAAGGAAACA 
AAAGCCCCAG CAGCTGAAAA ATTAGAGCCC AAAGCTGAAC AAGCTCCAAC AGTAAAAAGA 
ACAGAAACAG AAAAAAAGCC ACCACCTATT AAGGATAGCA AATCTTTAAC AGCTGAGCCT 
CAAAAGGCTG TCCTTCCCAC AAAACTGGAG AAATCGCCCA AACCAGAATC AACCTGTCCT 
CTCTGCAAAA CTGAACTCAA CATAGGTTCT AAGGATCCTC CTAACTTCAA TACTTGCACT 
GAATGCAAGA ATCAAGTGTG TAATCTCTGT GGATTTAACC CTACACCACA TTTGACTGAG 
ATTCAAGAAT GGCTTTGTTT AAATTGCCAA ACCCAGAGAG CAATATCAGG ACAGCTTGGA 
GACATACGCA AAATGCCACC TGCACCATCA GGACCCAAAG CATCTCCTAT GCCTGTTCCT 
ACAGAATCAT CATCTCAGAA AACAGCAGTG CCTCCCCAAG TAAAATTAGT GAAAAAGCAA 
GAACAAGAAG TAAAAACGGA AGCTGAAAAA GTCATTCTGG AAAAAGTAAA GGAAACACTA 
TCAATGGAAA AAATTCCTCC TATGGTAACC ACAGATCAAA AACAAGAAGA GAGTAAACTA 
GAGAAAGACA AAGCTTCAGC TCTTCAAGAA AAAAAGCCAC TCCCTGAAGA AAAAAAACTA 
ATCCCTGAAG AAGAAAAGAT ACGTTCTGAA GAAAAAAAGC CACTCCTAGA AGAAAAAAAG 
CCAACCCCTG AAGACAAAAA GCTACTCCCA GAGGCAAAAA CATCAGCCCC AGAAGAACAG 
AAACATGACT TACTTAAATC TCAAGTACAA ATTGCTGAAG AAAAGCTTGA AGGCAGAGTG 
GCTCCAAAGA CAGTGCAAGA AGGGAAACAA CCACAGACCA AGATGGAAGG TTTACCATCT 
GGCACACCTC AGAGTTTACC TAAAGAAGAT GATAAGACAA CCAAAACAAT AAAAGAACAG 
CCACAGCCAC CATGCACAGC AAAACCTGAT CAGGTGGAAC CTGGGAAAGA AAAAACAACT 
AAAGGTTCTC AAACCATGAC ATCCTCTGGA GCCCAGAAAA AAGTTAAAAG AACTCTGCCA 
AATCCACCTC CTGAGGAGAT TTCCACAGGA ACTCAATCCA CATTCAGCAC AATGGGCACA 
GTTTCCAGGA GAAGGATCTG CAGAACCAAC ACAATGGCAC GAGCCAAGAT TCTCCAGGAC 
ATAGACAGAG AGCTTGATCT TGTGGAAAGG GAGTCTGCAA AACTTCGAAA GAAACAAGCA 
GAGCTTGATG AAGAAGAAAA GGAGATTGAT GCTAAGCTAC GATACCTGGA AATGGGAATT 
AACAGGAGGA AAGAGGCCCT ATTAAAGGAG AGAGAAAAGA GAGAACGAGC CTACCTCCAG 
GGAGTAGCTG AGGATCGTGA TTACATGTCT GACAGTGAAG TGAGTAGCAC AAGACCAACC 
CGAATAGAAA GTCAGCATGG CATTGAGCGA CCAAGAACTG CTCCCCAAAC TGAATTCAGC 
CAGTTTATAC CACCACAAAC CCAAACAGAA TCTCAACTAG TTCCTCCGAC AAGTCCTTAC 
ACACAATACC AGTACTCTTC CCCTGCTCTT CCTACCCAAG CACCCACCTC ATACACTCAA 
CAGTCTCATT TTGAGCAACA AACTTTGTAC CATCAGCAAG TTTCACCTTA TCAGACTCAG 
CCAACATTCC AAGCTGTGGC AACAATGTCC TTCACACCTC AAGTTCAACC TACACCAACC 
CCACAGCCTT CTTATCAGTT ACCTTCACAG ATGATGGTGA TACAACAGAA GCCACGGCAA 
ACTACATTAT ATTTGGAGCC CAAGATAACC TCAAACTATG AAGTGATTCG CAACCAACCC 
CTTATGATAG CACCTGTTTC TACGGATAAC ACATTTGCTG TTTCCCATCT TGGTAGTAAG 
TACAATAGTT TAGACTTGAG AATAGGTTTG GAGGAAAGAA GTAGCATGGC AAGCAGTCCA 
ATATCAAGCA TATCTGCAGA TTCTTTCTAT GCAGATATTG ATCACCATAC TCCACGAAAT 
TATGTCCTAA TTGACGACAT TGGAGAGATC ACCAAAGGAA CAGCGGCATT AAGCACCGCA 
TTTAGCCTTC ATGAAAAGGA TCTGTCAAAA ACAGACCGTC TCCTTCGAAC CACTGAGACA 
CGCCGGTCTC AAGAAGTGAC AGATTTCCTA GCACCTTTAC AGTCTTCCTC TAGATTGCAT 
AGTTATGTGA AGGCGGAGGA AGACCCAATG GAGGATCCTT ACGAGTTAAA GCTTCTGAAA 
CATCAGATTA AACAGGAATT TCGTAGAGGG ACAGAGAGCT TAGATCACCT TGCTGGTCTT 
TCTCATTATT ACCATGCTGA TACTAGCTAC AGACATTTTC CAAAATCTGA GAAGTATAGC 
ATCAGTAGAC TCACACTTGA AAAACAAGCA GCAAAACAAC TGCCAGCAGC CATACTTTAT 
CAAAAGCAGT CAAAGCATAA GAAATCACTA ATTGACCCTA AAATGTCAAA ATTTTCACCT 
ATTCAAGAAA GTAGAGACCT TGAACCTGAT TATTCAAGCT ATATGACTTC TAGCACTTCA 
TCTATTGGTG GCATTTCCTC CAGGGCAAGG CTCCTTCAAG ATGACATCAC TTTTGGCCTC 
AGAAAAAATA TTACAGACCA ACAAAAATTT ATGGGATCTT CTCTTGGCAC AGGACTGGGC 
ACATTAGGAA ATACCATACG CTCAGCTCTG CAGGATGAAG CGGATAAGCC ATACAGTAGT 
GGCAGCAGGT CCAGACCTTC CTCCAGACCT TCCTCTGTCT ATGGGCTTGA TTTATCAATT 
AAAAGGGATT CTTCTAGCTC TTCCCTAAGA CTGAAAGCTC AAGAGGCTGA AGCTCTAGAT 
GTTTCCTTTA GTCATGCATC ATCCTCTGCC AGAACTAAGC CGACCAGTTT GCCAATTAGT 
CAAAGTAGAG GAAGAATACC AATTGTGGCC CAGAATTCTG AAGAAGAAAG CCCACTCAGT 
CCTGTTGGCC AGCCAATGGG AATGGCCAGG GCTGCAGCTG GACCCCTGCC ACCAATATCT 
GCAGACACCA GGGATCAGTT TGGATCAAGC CACTCATTGC CTGAAGTTCA GCAACACATG 
AGGGAAGAAT CACGGACTCG AGGCTATGAC CGTGACATAG CATTCATCAT GGATGACTTC 
CAACATGCCA TGTCAGACAG TGAAGCCTAT CATCTGCGTC GTGAGGAAAC AGATTGGTTT 
GATAAACCCA GGGAGTCTCG TTTGGAAAAT GGACATGGTC TGGACCGAAA ACTGCCGGAA 
AGATTGGTCC ACTCTAGACC ACTCAGTCAA CATCAAGAGC AAATTATACA GATGAACGGG 
AAAACTATGC ACTACATCTT TCCTCACGCA AGGATAAAAA TAACAAGAGA CTCAAAGGAT 
CACACAGTTT CAGGTAATGG ATTAGGAATT AGAATTGTGG GTGGTAAAGA AATCCCGGGA 
CATAGTGGAG AAATTGGAGC CTATATTGCC AAGATTCTTC CTGGGGGAAG TGCGGAACAG 
ACGGGGAAGC TTATGGAAGG GATGCAAGTA TTGGAATGGA ATGGAATTCC CTTGACTTCT 
AAAACATATG AAGAAGTTCA GAGTATCATT AGTCAGCAAA GTGGGGAAGC AGAAATATGT 
GTAAGACTGG ACCTCAATAT GCTATCAGAT TCTGAAAATT CCCAGCATCT GGAACTTCAT 
GAGCCACCAA AAGCTGTGGA TAAGGCGAAA TCCCCAGGGG TTGATCCTAA GCAGTTGGCA 
GCAGAACTCC AGAAGGTTTC ACTACAGCAG TCACCGCTGG TTCTGTCATC AGTTGTTGAA 
AAAGGATCTC ATGTTCATTC AGGTCCTACA TCAGCAGGAT CCAGTTCCGT TCCCAGCCCT 
GGGCAACCAG GGTCCCCCTC AGTGAGCAAA AAGAAGCACG GCAGCAGCAA GCCTACCGAT 
GGAACAAAGG TTGTCTCTCA TCCAATTACA GGAGAAATTC AGCTTCAAAT TAACTATGAT 
CTTGGAAATC TCATAATACA TATTCTCCAA GCAAGAAATC TTGTTCCTCG AGACAACAAT 
GGTTATTCTG ACCCTTTTGT GAAAGTGTAC CTTCTTCCAG GGAGAGGTCA AGTCATGGTT 
GTCCAGAATG CAAGTGCTGA GTACAAGAGA AGGACTAAAC ATGTCCAGAA AAGTCTTAAT 
CCTGAGTGGA ATCAAACAGT AATTTATAAA AGTATTTCCA TGGAACAGCT CAAGAAGAAA 
ACACTGGAGG TGACAGTTTG GGATTATGAT AGATTTTCAT CCAACGACTT CCTTGGGGAG 
GTATTGATTG ATTTATCTAG CACATCTCAC CTCGATAACA CTCCAAGGTG GTATCCTCTC 
AAAGAACAGA CTGAAAGCAT TGATCATGGC AAGTCTCATT CCAGTCAGAG CAGCCAGCAG 
TCCCCAAAGC CATCTGTTAT CAAAAGCAGA AGCCATGGTA TCTTCCCTGA CCCATCAAAG 
GACATGCAGG TTCCCACCAT TGAGAAATCC CATAGTAGTC CTGGTAGCTC AAAATCATCA 
TCAGAAGGCC ATCTCCGTTC TCATGGACCA TCTCGCAGTC AAAGCAAAAC CAGCGTCACT 
CAGACCCACC TGGAAGATGC AGGGGCTGCC ATAGCTGCTG CCGAAGCTGC CGTGCAACAA 
CTCCGCATTC AACCAACAGC ACATAAAAGC GGTCAGTCTA ATCATGCCAG GAAGCAGCAC 
AGACACAGCA TAGCAGGAGT CCTCCCCATT CAAAGAACTC AGTCTGATAA TCTGCCTCCA 
CCAGCCAATG GCAACCAAGA CCAAAGCCAG CTAGCCCTCA GGAAGGTGAT GTCTGATGGA 
CCAGTCAAGC CAGAAGGAGC AAAGCCTCCC AATCACCGGC CTGCAGAAAG CTCTGTGTCC 
ACCGGCTCCT CGGGCAGCAG CTTTGGCAGT GGGTATAGCG TGGACAGTGA AGGAAGCAGC 
AGCACTGCAG GGGAGACTAA TCTATTTCCT ATTCCGAGGA TAGGGAAGAT GGGACAGAAT 
GGACAAGAGC CTGTAAAACA GCCAGGGGTA GGAGTAGGAC TAGCAGACAC TGAAGCTAAA 
ACTCAGGTAA TGGGAGAAAT CAAGATTGCA TTGAAGAAGG AAATGAAGAC AGATGGTGAA 
CAACTAATAG TTGAAATTCT CCAATGCAGA AATATTACAT ACAAATTTAA GTCTCCTGAT 
CATCTACCAG ATTTATATGT GAAAATATAT GTGATGAATA TTTCTACCCA AAAAAAGGTG 
ATCAAGAAAA AAACAAGAGT ATGCAGACAT GATCGAGAGC CTTCGTTTAA TGAAACTTTT 
CGATTCAGTC TAAGTCCTGC AGGACATTCT CTTCAGATTT TACTTTTCTC CAATGGAGGG 
AAGTTTATGA AAAAGACCTT GATTGGTGAA GCCTGTATCT GGCTTGACAA AGTGGATCTC 
AGAAAAAGAA TAGTCAACTG GCACAAACTT TTGGTCAGTC CTACTCAAAC GCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867495.1
CDS	1565..10081
Translation	MGNEASLEGEGLPEGLAAAAAAGGGASGAGSPSHTAIPAGMEADLSQLSEEERRQIAAVMSRAQGLPKGSVPPAAAESPSMHRKQELDSSHPPKQSGRPPDPGRPAQPGLSKSRTTDTFRSEQKLPGRSPSTISLKESKSRTDLKEEHKSSMMPGFLSEVNALSAVSSVVNKFNPFDLISDSEASQEETTKKQKVVQKEQGKPEGIIKPPLQQQPPKPIPKQQGPGRDPLQQDGTPKSISSQQPEKIKSQPPGTGKPIQGPTQTPQTDHAKLPLQRDASRPQTKQADIVRGESVKPSLPSPSKPPIQQPTPGKPPAQQPGHEKSQPGPAKPPAQPSGLTKPLAQQPGTVKPPVQPPGTTKPPAQPLGPAKPPAQQTGSEKPSSEQPGPKALAQPPGVGKTPAQQPGPAKPPTQQVGTPKPLAQQPGLQSPAKAPGPTKTPVQQPGPGKIPAQQAGPGKTSAQQTGPTKPPSQLPGPAKPPPQQPGPAKPPPQQPGSAKPPSQQPGSTKPPPQQPGPAKPSPQQPGSTKPPSQQPGSAKPSAQQPSPAKPSAQQSTKPVSQTGSGKPLQPPTVSPSAKQPPSQGLPKTICPLCNTTELLLHVPEKANFNTCTECQTTVCSLCGFNPNPHLTEVKEWLCLNCQMKRALGGDLAPVPSSPQPKLKTAPVTTTSAVSKSSPQPQQTSPKKDAAPKQDLSKAPEPKKPPPLVKQPTLHGSPSAKAKQPPEADSLSKPAPPKEPSVPSEQDKAPVADDKPKQPKMVKPTTDLVSSSSATTKPDIPSSKVQSQAEEKTTPPLKTDSAKPSQSFPPTGEKVSPFDSKAIPRPASDSKIISHPGPSSESKGQKQVDPVQKKEEPKKAQTKMSPKPDAKPMPKGSPTPPGPRPTAGQTVPTPQQSPKPQEQSRRFSLNLGSITDAPKSQPTTPQETVTGKLFGFGASIFSQASNLISTAGQPGPHSQSGPGAPMKQAPAPSQPPTSQGPPKSTGQAPPAPAKSIPVKKETKAPAAEKLEPKAEQAPTVKRTETEKKPPPIKDSKSLTAEPQKAVLPTKLEKSPKPESTCPLCKTELNIGSKDPPNFNTCTECKNQVCNLCGFNPTPHLTEIQEWLCLNCQTQRAISGQLGDIRKMPPAPSGPKASPMPVPTESSSQKTAVPPQVKLVKKQEQEVKTEAEKVILEKVKETLSMEKIPPMVTTDQKQEESKLEKDKASALQEKKPLPEEKKLIPEEEKIRSEEKKPLLEEKKPTPEDKKLLPEAKTSAPEEQKHDLLKSQVQIAEEKLEGRVAPKTVQEGKQPQTKMEGLPSGTPQSLPKEDDKTTKTIKEQPQPPCTAKPDQVEPGKEKTTKGSQTMTSSGAQKKVKRTLPNPPPEEISTGTQSTFSTMGTVSRRRICRTNTMARAKILQDIDRELDLVERESAKLRKKQAELDEEEKEIDAKLRYLEMGINRRKEALLKEREKRERAYLQGVAEDRDYMSDSEVSSTRPTRIESQHGIERPRTAPQTEFSQFIPPQTQTESQLVPPTSPYTQYQYSSPALPTQAPTSYTQQSHFEQQTLYHQQVSPYQTQPTFQAVATMSFTPQVQPTPTPQPSYQLPSQMMVIQQKPRQTTLYLEPKITSNYEVIRNQPLMIAPVSTDNTFAVSHLGSKYNSLDLRIGLEERSSMASSPISSISADSFYADIDHHTPRNYVLIDDIGEITKGTAALSTAFSLHEKDLSKTDRLLRTTETRRSQEVTDFLAPLQSSSRLHSYVKAEEDPMEDPYELKLLKHQIKQEFRRGTESLDHLAGLSHYYHADTSYRHFPKSEKYSISRLTLEKQAAKQLPAAILYQKQSKHKKSLIDPKMSKFSPIQESRDLEPDYSSYMTSSTSSIGGISSRARLLQDDITFGLRKNITDQQKFMGSSLGTGLGTLGNTIRSALQDEADKPYSSGSRSRPSSRPSSVYGLDLSIKRDSSSSSLRLKAQEAEALDVSFSHASSSARTKPTSLPISQSRGRIPIVAQNSEEESPLSPVGQPMGMARAAAGPLPPISADTRDQFGSSHSLPEVQQHMREESRTRGYDRDIAFIMDDFQHAMSDSEAYHLRREETDWFDKPRESRLENGHGLDRKLPERLVHSRPLSQHQEQIIQMNGKTMHYIFPHARIKITRDSKDHTVSGNGLGIRIVGGKEIPGHSGEIGAYIAKILPGGSAEQTGKLMEGMQVLEWNGIPLTSKTYEEVQSIISQQSGEAEICVRLDLNMLSDSENSQHLELHEPPKAVDKAKSPGVDPKQLAAELQKVSLQQSPLVLSSVVEKGSHVHSGPTSAGSSSVPSPGQPGSPSVSKKKHGSSKPTDGTKVVSHPITGEIQLQINYDLGNLIIHILQARNLVPRDNNGYSDPFVKVYLLPGRGQVMVVQNASAEYKRRTKHVQKSLNPEWNQTVIYKSISMEQLKKKTLEVTVWDYDRFSSNDFLGEVLIDLSSTSHLDNTPRWYPLKEQTESIDHGKSHSSQSSQQSPKPSVIKSRSHGIFPDPSKDMQVPTIEKSHSSPGSSKSSSEGHLRSHGPSRSQSKTSVTQTHLEDAGAAIAAAEAAVQQLRIQPTAHKSGQSNHARKQHRHSIAGVLPIQRTQSDNLPPPANGNQDQSQLALRKVMSDGPVKPEGAKPPNHRPAESSVSTGSSGSSFGSGYSVDSEGSSSTAGETNLFPIPRIGKMGQNGQEPVKQPGVGVGLADTEAKTQVMGEIKIALKKEMKTDGEQLIVEILQCRNITYKFKSPDHLPDLYVKIYVMNISTQKKVIKKKTRVCRHDREPSFNETFRFSLSPAGHSLQILLFSNGGKFMKKTLIGEACIWLDKVDLRKRIVNWHKLLVSPTQTH

Target ORF information:

RefSeq Version	XM_017012006.2
Organism	Homo sapiens(human)
Definition	Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012006.2

ORF Insert Sequence:

ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA 
GCGGCTGGAG GAGGAGCTAG CGGGGCGGGG AGCCCCTCTC ACACCGCGAT CCCGGCCGGC 
ATGGAGGCGG ATTTGAGCCA GCTGAGCGAA GAGGAGAGGA GACAGATCGC CGCTGTCATG 
TCAAGGGCGC AGGGGCTGCC CAAGGGAAGC GTCCCCCCGG CCGCTGCGGA GTCGCCTTCC 
ATGCACAGGA AACAAGAGTT GGATAGTAGT CATCCTCCAA AGCAATCAGG AAGACCCCCG 
GACCCTGGGC GTCCAGCTCA ACCTGGTCTC AGTAAAAGTA GAACTACAGA CACTTTCAGG 
TCAGAGCAGA AATTGCCTGG GAGGAGTCCT TCCACTATTA GCTTGAAAGA ATCAAAGTCC 
AGAACTGATT TAAAGGAAGA GCACAAGTCT AGTATGATGC CTGGCTTCCT CTCAGAGGTT 
AACGCTTTAA GTGCTGTTTC CTCTGTTGTA AATAAATTCA ACCCTTTTGA TTTGATATCA 
GACTCTGAGG CATCCCAGGA AGAAACCACC AAGAAACAAA AAGTGGTTCA GAAGGAGCAA 
GGAAAACCTG AAGGAATCAT AAAACCTCCT TTACAACAAC AGCCACCCAA GCCGATTCCT 
AAGCAGCAAG GACCTGGTAG GGATCCGCTT CAGCAGGATG GCACTCCCAA ATCAATATCT 
TCTCAACAAC CAGAAAAAAT TAAATCACAA CCTCCAGGTA CAGGAAAGCC AATTCAGGGT 
CCTACCCAGA CTCCTCAGAC AGACCATGCA AAATTGCCAC TTCAACGAGA TGCATCCAGG 
CCTCAGACTA AACAGGCAGA CATAGTAAGG GGAGAATCAG TTAAACCCTC ACTGCCAAGC 
CCATCCAAAC CACCTATTCA GCAACCAACT CCTGGAAAAC CTCCAGCACA GCAGCCTGGA 
CATGAAAAAT CACAGCCTGG GCCTGCAAAG CCCCCAGCTC AGCCCTCAGG GCTAACAAAG 
CCATTGGCTC AACAACCAGG GACAGTGAAA CCCCCAGTCC AGCCACCAGG GACAACAAAG 
CCTCCAGCTC AGCCTCTTGG TCCTGCTAAG CCTCCAGCTC AGCAGACTGG GTCAGAGAAG 
CCTTCATCGG AGCAGCCTGG GCCAAAGGCT TTAGCTCAGC CTCCTGGAGT TGGAAAGACT 
CCAGCTCAAC AGCCAGGGCC AGCAAAGCCT CCAACCCAGC AGGTGGGGAC ACCAAAACCC 
CTAGCTCAAC AACCTGGGCT ACAGTCTCCA GCTAAGGCAC CTGGGCCTAC AAAGACTCCA 
GTTCAGCAGC CTGGGCCAGG AAAGATTCCA GCTCAACAGG CAGGACCCGG AAAGACTTCT 
GCCCAGCAGA CTGGCCCAAC AAAGCCTCCT TCACAACTGC CTGGCCCAGC AAAGCCCCCA 
CCTCAACAGC CTGGCCCAGC AAAGCCCCCA CCTCAACAGC CTGGCTCAGC AAAGCCCCCA 
TCTCAACAGC CTGGCTCAAC AAAACCCCCA CCTCAACAGC CTGGCCCAGC AAAGCCCTCA 
CCTCAACAGC CTGGCTCAAC AAAACCCCCA TCTCAACAGC CTGGCTCAGC AAAACCCTCA 
GCTCAACAGC CTAGCCCAGC AAAGCCCTCG GCTCAGCAAT CTACAAAACC AGTAAGCCAA 
ACAGGATCTG GAAAACCTCT GCAGCCACCA ACAGTGTCTC CATCTGCAAA ACAGCCTCCT 
TCACAAGGCC TCCCTAAAAC CATCTGTCCT CTTTGCAATA CCACTGAACT TCTGTTGCAT 
GTTCCAGAAA AGGCCAATTT TAACACATGC ACTGAGTGTC AAACCACTGT CTGTAGTCTC 
TGTGGTTTTA ATCCCAATCC TCATTTAACG GAGGTAAAAG AGTGGCTCTG TTTGAACTGT 
CAAATGAAAA GAGCTCTAGG CGGGGATCTG GCTCCAGTTC CGTCATCACC CCAGCCCAAA 
CTGAAGACTG CACCTGTTAC CACTACATCA GCAGTGAGCA AATCATCCCC ACAGCCACAG 
CAGACTTCCC CAAAGAAGGA TGCTGCACCA AAACAGGATC TCTCCAAGGC ACCTGAGCCT 
AAAAAGCCAC CACCACTAGT GAAACAACCA ACCCTTCATG GCTCTCCTTC AGCCAAGGCC 
AAGCAGCCCC CTGAGGCAGA TTCTTTGTCC AAGCCAGCCC CTCCCAAAGA ACCTTCTGTC 
CCATCTGAGC AGGACAAGGC CCCTGTTGCT GATGATAAAC CAAAGCAGCC CAAGATGGTA 
AAGCCAACCA CTGACCTTGT ATCTTCATCA TCAGCAACAA CAAAACCTGA TATTCCAAGC 
TCCAAAGTAC AGTCACAAGC TGAAGAGAAA ACAACCCCTC CTCTAAAAAC AGACTCTGCC 
AAACCCTCAC AGAGTTTTCC ACCAACAGGG GAAAAAGTCA GCCCATTTGA TTCTAAAGCC 
ATACCTCGAC CTGCATCAGA TTCAAAAATT ATTTCACATC CTGGTCCCAG TTCAGAGAGC 
AAAGGTCAAA AACAAGTTGA CCCCGTACAA AAGAAGGAAG AACCCAAGAA AGCACAAACC 
AAAATGAGTC CTAAACCAGA TGCCAAGCCA ATGCCAAAAG GGTCACCAAC ACCCCCTGGC 
CCACGACCTA CCGCTGGCCA AACTGTCCCC ACACCTCAAC AGTCCCCAAA GCCTCAGGAG 
CAGTCAAGGC GTTTCAGTCT GAATCTGGGA AGTATTACTG ATGCCCCCAA ATCACAGCCT 
ACAACTCCTC AAGAGACCGT GACTGGGAAA CTCTTTGGGT TTGGAGCATC AATCTTCAGC 
CAGGCATCAA ATTTAATTTC CACTGCAGGC CAACCTGGAC CTCATTCACA AAGTGGACCA 
GGGGCCCCAA TGAAACAAGC CCCTGCCCCT TCACAGCCAC CTACTTCACA AGGGCCACCC 
AAATCCACAG GTCAAGCACC ACCAGCACCT GCAAAAAGTA TACCTGTGAA AAAGGAAACA 
AAAGCCCCAG CAGCTGAAAA ATTAGAGCCC AAAGCTGAAC AAGCTCCAAC AGTAAAAAGA 
ACAGAAACAG AAAAAAAGCC ACCACCTATT AAGGATAGCA AATCTTTAAC AGCTGAGCCT 
CAAAAGGCTG TCCTTCCCAC AAAACTGGAG AAATCGCCCA AACCAGAATC AACCTGTCCT 
CTCTGCAAAA CTGAACTCAA CATAGGTTCT AAGGATCCTC CTAACTTCAA TACTTGCACT 
GAATGCAAGA ATCAAGTGTG TAATCTCTGT GGATTTAACC CTACACCACA TTTGACTGAG 
ATTCAAGAAT GGCTTTGTTT AAATTGCCAA ACCCAGAGAG CAATATCAGG ACAGCTTGGA 
GACATACGCA AAATGCCACC TGCACCATCA GGACCCAAAG CATCTCCTAT GCCTGTTCCT 
ACAGAATCAT CATCTCAGAA AACAGCAGTG CCTCCCCAAG TAAAATTAGT GAAAAAGCAA 
GAACAAGAAG TAAAAACGGA AGCTGAAAAA GTCATTCTGG AAAAAGTAAA GGAAACACTA 
TCAATGGAAA AAATTCCTCC TATGGTAACC ACAGATCAAA AACAAGAAGA GAGTAAACTA 
GAGAAAGACA AAGCTTCAGC TCTTCAAGAA AAAAAGCCAC TCCCTGAAGA AAAAAAACTA 
ATCCCTGAAG AAGAAAAGAT ACGTTCTGAA GAAAAAAAGC CACTCCTAGA AGAAAAAAAG 
CCAACCCCTG AAGACAAAAA GCTACTCCCA GAGGCAAAAA CATCAGCCCC AGAAGAACAG 
AAACATGACT TACTTAAATC TCAAGTACAA ATTGCTGAAG AAAAGCTTGA AGGCAGAGTG 
GCTCCAAAGA CAGTGCAAGA AGGGAAACAA CCACAGACCA AGATGGAAGG TTTACCATCT 
GGCACACCTC AGAGTTTACC TAAAGAAGAT GATAAGACAA CCAAAACAAT AAAAGAACAG 
CCACAGCCAC CATGCACAGC AAAACCTGAT CAGGTGGAAC CTGGGAAAGA AAAAACAACT 
AAAGGTTCTC AAACCATGAC ATCCTCTGGA GCCCAGAAAA AAGTTAAAAG AACTCTGCCA 
AATCCACCTC CTGAGGAGAT TTCCACAGGA ACTCAATCCA CATTCAGCAC AATGGGCACA 
GTTTCCAGGA GAAGGATCTG CAGAACCAAC ACAATGGCAC GAGCCAAGAT TCTCCAGGAC 
ATAGACAGAG AGCTTGATCT TGTGGAAAGG GAGTCTGCAA AACTTCGAAA GAAACAAGCA 
GAGCTTGATG AAGAAGAAAA GGAGATTGAT GCTAAGCTAC GATACCTGGA AATGGGAATT 
AACAGGAGGA AAGAGGCCCT ATTAAAGGAG AGAGAAAAGA GAGAACGAGC CTACCTCCAG 
GGAGTAGCTG AGGATCGTGA TTACATGTCT GACAGTGAAG TGAGTAGCAC AAGACCAACC 
CGAATAGAAA GTCAGCATGG CATTGAGCGA CCAAGAACTG CTCCCCAAAC TGAATTCAGC 
CAGTTTATAC CACCACAAAC CCAAACAGAA TCTCAACTAG TTCCTCCGAC AAGTCCTTAC 
ACACAATACC AGTACTCTTC CCCTGCTCTT CCTACCCAAG CACCCACCTC ATACACTCAA 
CAGTCTCATT TTGAGCAACA AACTTTGTAC CATCAGCAAG TTTCACCTTA TCAGACTCAG 
CCAACATTCC AAGCTGTGGC AACAATGTCC TTCACACCTC AAGTTCAACC TACACCAACC 
CCACAGCCTT CTTATCAGTT ACCTTCACAG ATGATGGTGA TACAACAGAA GCCACGGCAA 
ACTACATTAT ATTTGGAGCC CAAGATAACC TCAAACTATG AAGTGATTCG CAACCAACCC 
CTTATGATAG CACCTGTTTC TACGGATAAC ACATTTGCTG TTTCCCATCT TGGTAGTAAG 
TACAATAGTT TAGACTTGAG AATAGGTTTG GAGGAAAGAA GTAGCATGGC AAGCAGTCCA 
ATATCAAGCA TATCTGCAGA TTCTTTCTAT GCAGATATTG ATCACCATAC TCCACGAAAT 
TATGTCCTAA TTGACGACAT TGGAGAGATC ACCAAAGGAA CAGCGGCATT AAGCACCGCA 
TTTAGCCTTC ATGAAAAGGA TCTGTCAAAA ACAGACCGTC TCCTTCGAAC CACTGAGACA 
CGCCGGTCTC AAGAAGTGAC AGATTTCCTA GCACCTTTAC AGTCTTCCTC TAGATTGCAT 
AGTTATGTGA AGGCGGAGGA AGACCCAATG GAGGATCCTT ACGAGTTAAA GCTTCTGAAA 
CATCAGATTA AACAGGAATT TCGTAGAGGG ACAGAGAGCT TAGATCACCT TGCTGGTCTT 
TCTCATTATT ACCATGCTGA TACTAGCTAC AGACATTTTC CAAAATCTGA GAAGTATAGC 
ATCAGTAGAC TCACACTTGA AAAACAAGCA GCAAAACAAC TGCCAGCAGC CATACTTTAT 
CAAAAGCAGT CAAAGCATAA GAAATCACTA ATTGACCCTA AAATGTCAAA ATTTTCACCT 
ATTCAAGAAA GTAGAGACCT TGAACCTGAT TATTCAAGCT ATATGACTTC TAGCACTTCA 
TCTATTGGTG GCATTTCCTC CAGGGCAAGG CTCCTTCAAG ATGACATCAC TTTTGGCCTC 
AGAAAAAATA TTACAGACCA ACAAAAATTT ATGGGATCTT CTCTTGGCAC AGGACTGGGC 
ACATTAGGAA ATACCATACG CTCAGCTCTG CAGGATGAAG CGGATAAGCC ATACAGTAGT 
GGCAGCAGGT CCAGACCTTC CTCCAGACCT TCCTCTGTCT ATGGGCTTGA TTTATCAATT 
AAAAGGGATT CTTCTAGCTC TTCCCTAAGA CTGAAAGCTC AAGAGGCTGA AGCTCTAGAT 
GTTTCCTTTA GTCATGCATC ATCCTCTGCC AGAACTAAGC CGACCAGTTT GCCAATTAGT 
CAAAGTAGAG GAAGAATACC AATTGTGGCC CAGAATTCTG AAGAAGAAAG CCCACTCAGT 
CCTGTTGGCC AGCCAATGGG AATGGCCAGG GCTGCAGCTG GACCCCTGCC ACCAATATCT 
GCAGACACCA GGGATCAGTT TGGATCAAGC CACTCATTGC CTGAAGTTCA GCAACACATG 
AGGGAAGAAT CACGGACTCG AGGCTATGAC CGTGACATAG CATTCATCAT GGATGACTTC 
CAACATGCCA TGTCAGACAG TGAAGCCTAT CATCTGCGTC GTGAGGAAAC AGATTGGTTT 
GATAAACCCA GGGAGTCTCG TTTGGAAAAT GGACATGGTC TGGACCGAAA ACTGCCGGAA 
AGATTGGTCC ACTCTAGACC ACTCAGTCAA CATCAAGAGC AAATTATACA GATGAACGGG 
AAAACTATGC ACTACATCTT TCCTCACGCA AGGATAAAAA TAACAAGAGA CTCAAAGGAT 
CACACAGTTT CAGGTAATGG ATTAGGAATT AGAATTGTGG GTGGTAAAGA AATCCCGGGA 
CATAGTGGAG AAATTGGAGC CTATATTGCC AAGATTCTTC CTGGGGGAAG TGCGGAACAG 
ACGGGGAAGC TTATGGAAGG GATGCAAGTA TTGGAATGGA ATGGAATTCC CTTGACTTCT 
AAAACATATG AAGAAGTTCA GAGTATCATT AGTCAGCAAA GTGGGGAAGC AGAAATATGT 
GTAAGACTGG ACCTCAATAT GCTATCAGAT TCTGAAAATT CCCAGCATCT GGAACTTCAT 
GAGCCACCAA AAGCTGTGGA TAAGGCGAAA TCCCCAGGGG TTGATCCTAA GCAGTTGGCA 
GCAGAACTCC AGAAGGTTTC ACTACAGCAG TCACCGCTGG TTCTGTCATC AGTTGTTGAA 
AAAGGATCTC ATGTTCATTC AGGTCCTACA TCAGCAGGAT CCAGTTCCGT TCCCAGCCCT 
GGGCAACCAG GGTCCCCCTC AGTGAGCAAA AAGAAGCACG GCAGCAGCAA GCCTACCGAT 
GGAACAAAGG TTGTCTCTCA TCCAATTACA GGAGAAATTC AGCTTCAAAT TAACTATGAT 
CTTGGAAATC TCATAATACA TATTCTCCAA GCAAGAAATC TTGTTCCTCG AGACAACAAT 
GGTTATTCTG ACCCTTTTGT GAAAGTGTAC CTTCTTCCAG GGAGAGGTCA AGTCATGGTT 
GTCCAGAATG CAAGTGCTGA GTACAAGAGA AGGACTAAAC ATGTCCAGAA AAGTCTTAAT 
CCTGAGTGGA ATCAAACAGT AATTTATAAA AGTATTTCCA TGGAACAGCT CAAGAAGAAA 
ACACTGGAGG TGACAGTTTG GGATTATGAT AGATTTTCAT CCAACGACTT CCTTGGGGAG 
GTATTGATTG ATTTATCTAG CACATCTCAC CTCGATAACA CTCCAAGGTG GTATCCTCTC 
AAAGAACAGA CTGAAAGCAT TGATCATGGC AAGTCTCATT CCAGTCAGAG CAGCCAGCAG 
TCCCCAAAGC CATCTGTTAT CAAAAGCAGA AGCCATGGTA TCTTCCCTGA CCCATCAAAG 
GACATGCAGG TTCCCACCAT TGAGAAATCC CATAGTAGTC CTGGTAGCTC AAAATCATCA 
TCAGAAGGCC ATCTCCGTTC TCATGGACCA TCTCGCAGTC AAAGCAAAAC CAGCGTCACT 
CAGACCCACC TGGAAGATGC AGGGGCTGCC ATAGCTGCTG CCGAAGCTGC CGTGCAACAA 
CTCCGCATTC AACCAACAGC ACATAAAAGC GGTCAGTCTA ATCATGCCAG GAAGCAGCAC 
AGACACAGCA TAGCAGGAGT CCTCCCCATT CAAAGAACTC AGTCTGATAA TCTGCCTCCA 
CCAGCCAATG GCAACCAAGA CCAAAGCCAG CTAGCCCTCA GGAAGGTGAT GTCTGATGGA 
CCAGTCAAGC CAGAAGGAGC AAAGCCTCCC AATCACCGGC CTGCAGAAAG CTCTGTGTCC 
ACCGGCTCCT CGGGCAGCAG CTTTGGCAGT GGGTATAGCG TGGACAGTGA AGGAAGCAGC 
AGCACTGCAG GGGAGACTAA TCTATTTCCT ATTCCGAGGA TAGGGAAGAT GGGACAGAAT 
GGACAAGAGC CTGTAAAACA GCCAGGGGTA GGAGTAGGAC TAGCAGACAC TGAAGCTAAA 
ACTCAGGTAA TGGGAGAAAT CAAGATTGCA TTGAAGAAGG AAATGAAGAC AGATGGTGAA 
CAACTAATAG TTGAAATTCT CCAATGCAGA AATATTACAT ACAAATTTAA GTCTCCTGAT 
CATCTACCAG ATTTATATGT GAAAATATAT GTGATGAATA TTTCTACCCA AAAAAAGGTG 
ATCAAGAAAA AAACAAGAGT ATGCAGACAT GATCGAGAGC CTTCGTTTAA TGAAACTTTT 
CGATTCAGTC TAAGTCCTGC AGGACATTCT CTTCAGATTT TACTTTTCTC CAATGGAGGG 
AAGTTTATGA AAAAGACCTT GATTGGTGAA GCCTGTATCT GGCTTGACAA AGTGGATCTC 
AGAAAAAGAA TAGTCAACTG GCACAAACTT TTGGTCAGTC CTACTCAAAC GCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCLO ( XM_017012007.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867496.1 Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant X3, mRNA.

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CloneID	OHu97175
Clone ID Related Accession (Same CDS sequence)	XM_017012007.1
Accession Version	XM_017012007.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein piccolo isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA 
GCGGCTGGAG GAGGAGCTAG CGGGGCGGGG AGCCCCTCTC ACACCGCGAT CCCGGCCGGC 
ATGGAGGCGG ATTTGAGCCA GCTGAGCGAA GAGGAGAGGA GACAGATCGC CGCTGTCATG 
TCAAGGGCGC AGGGGCTGCC CAAGGGAAGC GTCCCCCCGG CCGCTGCGGA GTCGCCTTCC 
ATGCACAGGA AACAAGAGTT GGATAGTAGT CATCCTCCAA AGCAATCAGG AAGACCCCCG 
GACCCTGGGC GTCCAGCTCA ACCTGGTCTC AGTAAAAGTA GAACTACAGA CACTTTCAGG 
TCAGAGCAGA AATTGCCTGG GAGGAGTCCT TCCACTATTA GCTTGAAAGA ATCAAAGTCC 
AGAACTGATT TAAAGGAAGA GCACAAGTCT AGTATGATGC CTGGCTTCCT CTCAGAGGTT 
AACGCTTTAA GTGCTGTTTC CTCTGTTGTA AATAAATTCA ACCCTTTTGA TTTGATATCA 
GACTCTGAGG CATCCCAGGA AGAAACCACC AAGAAACAAA AAGTGGTTCA GAAGGAGCAA 
GGAAAACCTG AAGGAATCAT AAAACCTCCT TTACAACAAC AGCCACCCAA GCCGATTCCT 
AAGCAGCAAG GACCTGGTAG GGATCCGCTT CAGCAGGATG GCACTCCCAA ATCAATATCT 
TCTCAACAAC CAGAAAAAAT TAAATCACAA CCTCCAGGTA CAGGAAAGCC AATTCAGGGT 
CCTACCCAGA CTCCTCAGAC AGACCATGCA AAATTGCCAC TTCAACGAGA TGCATCCAGG 
CCTCAGACTA AACAGGCAGA CATAGTAAGG GGAGAATCAG TTAAACCCTC ACTGCCAAGC 
CCATCCAAAC CACCTATTCA GCAACCAACT CCTGGAAAAC CTCCAGCACA GCAGCCTGGA 
CATGAAAAAT CACAGCCTGG GCCTGCAAAG CCCCCAGCTC AGCCCTCAGG GCTAACAAAG 
CCATTGGCTC AACAACCAGG GACAGTGAAA CCCCCAGTCC AGCCACCAGG GACAACAAAG 
CCTCCAGCTC AGCCTCTTGG TCCTGCTAAG CCTCCAGCTC AGCAGACTGG GTCAGAGAAG 
CCTTCATCGG AGCAGCCTGG GCCAAAGGCT TTAGCTCAGC CTCCTGGAGT TGGAAAGACT 
CCAGCTCAAC AGCCAGGGCC AGCAAAGCCT CCAACCCAGC AGGTGGGGAC ACCAAAACCC 
CTAGCTCAAC AACCTGGGCT ACAGTCTCCA GCTAAGGCAC CTGGGCCTAC AAAGACTCCA 
GTTCAGCAGC CTGGGCCAGG AAAGATTCCA GCTCAACAGG CAGGACCCGG AAAGACTTCT 
GCCCAGCAGA CTGGCCCAAC AAAGCCTCCT TCACAACTGC CTGGCCCAGC AAAGCCCCCA 
CCTCAACAGC CTGGCCCAGC AAAGCCCCCA CCTCAACAGC CTGGCTCAGC AAAGCCCCCA 
TCTCAACAGC CTGGCTCAAC AAAACCCCCA CCTCAACAGC CTGGCCCAGC AAAGCCCTCA 
CCTCAACAGC CTGGCTCAAC AAAACCCCCA TCTCAACAGC CTGGCTCAGC AAAACCCTCA 
GCTCAACAGC CTAGCCCAGC AAAGCCCTCG GCTCAGCAAT CTACAAAACC AGTAAGCCAA 
ACAGGATCTG GAAAACCTCT GCAGCCACCA ACAGTGTCTC CATCTGCAAA ACAGCCTCCT 
TCACAAGGCC TCCCTAAAAC CATCTGTCCT CTTTGCAATA CCACTGAACT TCTGTTGCAT 
GTTCCAGAAA AGGCCAATTT TAACACATGC ACTGAGTGTC AAACCACTGT CTGTAGTCTC 
TGTGGTTTTA ATCCCAATCC TCATTTAACG GAGGTAAAAG AGTGGCTCTG TTTGAACTGT 
CAAATGAAAA GAGCTCTAGG CGGGGATCTG GCTCCAGTTC CGTCATCACC CCAGCCCAAA 
CTGAAGACTG CACCTGTTAC CACTACATCA GCAGTGAGCA AATCATCCCC ACAGCCACAG 
CAGACTTCCC CAAAGAAGGA TGCTGCACCA AAACAGGATC TCTCCAAGGC ACCTGAGCCT 
AAAAAGCCAC CACCACTAGT GAAACAACCA ACCCTTCATG GCTCTCCTTC AGCCAAGGCC 
AAGCAGCCCC CTGAGGCAGA TTCTTTGTCC AAGCCAGCCC CTCCCAAAGA ACCTTCTGTC 
CCATCTGAGC AGGACAAGGC CCCTGTTGCT GATGATAAAC CAAAGCAGCC CAAGATGGTA 
AAGCCAACCA CTGACCTTGT ATCTTCATCA TCAGCAACAA CAAAACCTGA TATTCCAAGC 
TCCAAAGTAC AGTCACAAGC TGAAGAGAAA ACAACCCCTC CTCTAAAAAC AGACTCTGCC 
AAACCCTCAC AGAGTTTTCC ACCAACAGGG GAAAAAGTCA GCCCATTTGA TTCTAAAGCC 
ATACCTCGAC CTGCATCAGA TTCAAAAATT ATTTCACATC CTGGTCCCAG TTCAGAGAGC 
AAAGGTCAAA AACAAGTTGA CCCCGTACAA AAGAAGGAAG AACCCAAGAA AGCACAAACC 
AAAATGAGTC CTAAACCAGA TGCCAAGCCA ATGCCAAAAG GGTCACCAAC ACCCCCTGGC 
CCACGACCTA CCGCTGGCCA AACTGTCCCC ACACCTCAAC AGTCCCCAAA GCCTCAGGAG 
CAGTCAAGGC GTTTCAGTCT GAATCTGGGA AGTATTACTG ATGCCCCCAA ATCACAGCCT 
ACAACTCCTC AAGAGACCGT GACTGGGAAA CTCTTTGGGT TTGGAGCATC AATCTTCAGC 
CAGGCATCAA ATTTAATTTC CACTGCAGGC CAACCTGGAC CTCATTCACA AAGTGGACCA 
GGGGCCCCAA TGAAACAAGC CCCTGCCCCT TCACAGCCAC CTACTTCACA AGGGCCACCC 
AAATCCACAG GTCAAGCACC ACCAGCACCT GCAAAAAGTA TACCTGTGAA AAAGGAAACA 
AAAGCCCCAG CAGCTGAAAA ATTAGAGCCC AAAGCTGAAC AAGCTCCAAC AGTAAAAAGA 
ACAGAAACAG AAAAAAAGCC ACCACCTATT AAGGATAGCA AATCTTTAAC AGCTGAGCCT 
CAAAAGGCTG TCCTTCCCAC AAAACTGGAG AAATCGCCCA AACCAGAATC AACCTGTCCT 
CTCTGCAAAA CTGAACTCAA CATAGGTTCT AAGGATCCTC CTAACTTCAA TACTTGCACT 
GAATGCAAGA ATCAAGTGTG TAATCTCTGT GGATTTAACC CTACACCACA TTTGACTGAG 
ATTCAAGAAT GGCTTTGTTT AAATTGCCAA ACCCAGAGAG CAATATCAGG ACAGCTTGGA 
GACATACGCA AAATGCCACC TGCACCATCA GGACCCAAAG CATCTCCTAT GCCTGTTCCT 
ACAGAATCAT CATCTCAGAA AACAGCAGTG CCTCCCCAAG TAAAATTAGT GAAAAAGCAA 
GAACAAGAAG TAAAAACGGA AGCTGAAAAA GTCATTCTGG AAAAAGTAAA GGAAACACTA 
TCAATGGAAA AAATTCCTCC TATGGTAACC ACAGATCAAA AACAAGAAGA GAGTAAACTA 
GAGAAAGACA AAGCTTCAGC TCTTCAAGAA AAAAAGCCAC TCCCTGAAGA AAAAAAACTA 
ATCCCTGAAG AAGAAAAGAT ACGTTCTGAA GAAAAAAAGC CACTCCTAGA AGAAAAAAAG 
CCAACCCCTG AAGACAAAAA GCTACTCCCA GAGGCAAAAA CATCAGCCCC AGAAGAACAG 
AAACATGACT TACTTAAATC TCAAGTACAA ATTGCTGAAG AAAAGCTTGA AGGCAGAGTG 
GCTCCAAAGA CAGTGCAAGA AGGGAAACAA CCACAGACCA AGATGGAAGG TTTACCATCT 
GGCACACCTC AGAGTTTACC TAAAGAAGAT GATAAGACAA CCAAAACAAT AAAAGAACAG 
CCACAGCCAC CATGCACAGC AAAACCTGAT CAGGTGGAAC CTGGGAAAGA AAAAACAACT 
AAAGGTTCTC AAACCATGAC ATCCTCTGGA GCCCAGAAAA AAGTTAAAAG AACTCTGCCA 
AATCCACCTC CTGAGGAGAT TTCCACAGGA ACTCAATCCA CATTCAGCAC AATGGGCACA 
GTTTCCAGGA GAAGGATCTG CAGAACCAAC ACAATGGCAC GAGCCAAGAT TCTCCAGGAC 
ATAGACAGAG AGCTTGATCT TGTGGAAAGG GAGTCTGCAA AACTTCGAAA GAAACAAGCA 
GAGCTTGATG AAGAAGAAAA GGAGATTGAT GCTAAGCTAC GATACCTGGA AATGGGAATT 
AACAGGAGGA AAGAGGCCCT ATTAAAGGAG AGAGAAAAGA GAGAACGAGC CTACCTCCAG 
GGAGTAGCTG AGGATCGTGA TTACATGTCT GACAGTGAAG TGAGTAGCAC AAGACCAACC 
CGAATAGAAA GTCAGCATGG CATTGAGCGA CCAAGAACTG CTCCCCAAAC TGAATTCAGC 
CAGTTTATAC CACCACAAAC CCAAACAGAA TCTCAACTAG TTCCTCCGAC AAGTCCTTAC 
ACACAATACC AGTACTCTTC CCCTGCTCTT CCTACCCAAG CACCCACCTC ATACACTCAA 
CAGTCTCATT TTGAGCAACA AACTTTGTAC CATCAGCAAG TTTCACCTTA TCAGACTCAG 
CCAACATTCC AAGCTGTGGC AACAATGTCC TTCACACCTC AAGTTCAACC TACACCAACC 
CCACAGCCTT CTTATCAGTT ACCTTCACAG ATGATGGTGA TACAACAGAA GCCACGGCAA 
ACTACATTAT ATTTGGAGCC CAAGATAACC TCAAACTATG AAGTGATTCG CAACCAACCC 
CTTATGATAG CACCTGTTTC TACGGATAAC ACATTTGCTG TTTCCCATCT TGGTAGTAAG 
TACAATAGTT TAGACTTGAG AATAGGTTTG GAGGAAAGAA GTAGCATGGC AAGCAGTCCA 
ATATCAAGCA TATCTGCAGA TTCTTTCTAT GCAGATATTG ATCACCATAC TCCACGAAAT 
TATGTCCTAA TTGACGACAT TGGAGAGATC ACCAAAGGAA CAGCGGCATT AAGCACCGCA 
TTTAGCCTTC ATGAAAAGGA TCTGTCAAAA ACAGACCGTC TCCTTCGAAC CACTGAGACA 
CGCCGGTCTC AAGAAGTGAC AGATTTCCTA GCACCTTTAC AGTCTTCCTC TAGATTGCAT 
AGTTATGTGA AGGCGGAGGA AGACCCAATG GAGGATCCTT ACGAGTTAAA GCTTCTGAAA 
CATCAGATTA AACAGGAATT TCGTAGAGGG ACAGAGAGCT TAGATCACCT TGCTGGTCTT 
TCTCATTATT ACCATGCTGA TACTAGCTAC AGACATTTTC CAAAATCTGA GAAGTATAGC 
ATCAGTAGAC TCACACTTGA AAAACAAGCA GCAAAACAAC TGCCAGCAGC CATACTTTAT 
CAAAAGCAGT CAAAGCATAA GAAATCACTA ATTGACCCTA AAATGTCAAA ATTTTCACCT 
ATTCAAGAAA GTAGAGACCT TGAACCTGAT TATTCAAGCT ATATGACTTC TAGCACTTCA 
TCTATTGGTG GCATTTCCTC CAGGGCAAGG CTCCTTCAAG ATGACATCAC TTTTGGCCTC 
AGAAAAAATA TTACAGACCA ACAAAAATTT ATGGGATCTT CTCTTGGCAC AGGACTGGGC 
ACATTAGGAA ATACCATACG CTCAGCTCTG CAGGATGAAG CGGATAAGCC ATACAGTAGT 
GGCAGCAGGT CCAGACCTTC CTCCAGACCT TCCTCTGTCT ATGGGCTTGA TTTATCAATT 
AAAAGGGATT CTTCTAGCTC TTCCCTAAGA CTGAAAGCTC AAGAGGCTGA AGCTCTAGAT 
GTTTCCTTTA GTCATGCATC ATCCTCTGCC AGAACTAAGC CGACCAGTTT GCCAATTAGT 
CAAAGTAGAG GAAGAATACC AATTGTGGCC CAGAATTCTG AAGAAGAAAG CCCACTCAGT 
CCTGTTGGCC AGCCAATGGG AATGGCCAGG GCTGCAGCTG GACCCCTGCC ACCAATATCT 
GCAGACACCA GGGATCAGTT TGGATCAAGC CACTCATTGC CTGAAGTTCA GCAACACATG 
AGGGAAGAAT CACGGACTCG AGGCTATGAC CGTGACATAG CATTCATCAT GGATGACTTC 
CAACATGCCA TGTCAGACAG TGAAGCCTAT CATCTGCGTC GTGAGGAAAC AGATTGGTTT 
GATAAACCCA GGGAGTCTCG TTTGGAAAAT GGACATGGTC TGGACCGAAA ACTGCCGGAA 
AGATTGGTCC ACTCTAGACC ACTCAGTCAA CATCAAGAGC AAATTATACA GATGAACGGG 
AAAACTATGC ACTACATCTT TCCTCACGCA AGGATAAAAA TAACAAGAGA CTCAAAGGAT 
CACACAGTTT CAGGTAATGG ATTAGGAATT AGAATTGTGG GTGGTAAAGA AATCCCGGGA 
CATAGTGGAG AAATTGGAGC CTATATTGCC AAGATTCTTC CTGGGGGAAG TGCGGAACAG 
ACGGGGAAGC TTATGGAAGG GATGCAAGTA TTGGAATGGA ATGGAATTCC CTTGACTTCT 
AAAACATATG AAGAAGTTCA GAGTATCATT AGTCAGCAAA GTGGGGAAGC AGAAATATGT 
GTAAGACTGG ACCTCAATAT GCTATCAGAT TCTGAAAATT CCCAGCATCT GGAACTTCAT 
GAGCCACCAA AAGCTGTGGA TAAGGCGAAA TCCCCAGGGG TTGATCCTAA GCAGTTGGCA 
GCAGAACTCC AGAAGGTTTC ACTACAGCAG TCACCGCTGG TTCTGTCATC AGTTGTTGAA 
AAAGGATCTC ATGTTCATTC AGGTCCTACA TCAGCAGGAT CCAGTTCCGT TCCCAGCCCT 
GGGCAACCAG GGTCCCCCTC AGTGAGCAAA AAGAAGCACG GCAGCAGCAA GCCTACCGAT 
GGAACAAAGG TTGTCTCTCA TCCAATTACA GGAGAAATTC AGCTTCAAAT TAACTATGAT 
CTTGGAAATC TCATAATACA TATTCTCCAA GCAAGAAATC TTGTTCCTCG AGACAACAAT 
GGTTATTCTG ACCCTTTTGT GAAAGTGTAC CTTCTTCCAG GGAGAGGTGC TGAGTACAAG 
AGAAGGACTA AACATGTCCA GAAAAGTCTT AATCCTGAGT GGAATCAAAC AGTAATTTAT 
AAAAGTATTT CCATGGAACA GCTCAAGAAG AAAACACTGG AGGTGACAGT TTGGGATTAT 
GATAGATTTT CATCCAACGA CTTCCTTGGG GAGGTATTGA TTGATTTATC TAGCACATCT 
CACCTCGATA ACACTCCAAG GTGGTATCCT CTCAAAGAAC AGACTGAAAG CATTGATCAT 
GGCAAGTCTC ATTCCAGTCA GAGCAGCCAG CAGTCCCCAA AGCCATCTGT TATCAAAAGC 
AGAAGCCATG GTATCTTCCC TGACCCATCA AAGGACATGC AGGTTCCCAC CATTGAGAAA 
TCCCATAGTA GTCCTGGTAG CTCAAAATCA TCATCAGAAG GCCATCTCCG TTCTCATGGA 
CCATCTCGCA GTCAAAGCAA AACCAGCGTC ACTCAGACCC ACCTGGAAGA TGCAGGGGCT 
GCCATAGCTG CTGCCGAAGC TGCCGTGCAA CAACTCCGCA TTCAACCAAG TAAAAGACGC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867496.1
CDS	1549..9234
Translation	MGNEASLEGEGLPEGLAAAAAAGGGASGAGSPSHTAIPAGMEADLSQLSEEERRQIAAVMSRAQGLPKGSVPPAAAESPSMHRKQELDSSHPPKQSGRPPDPGRPAQPGLSKSRTTDTFRSEQKLPGRSPSTISLKESKSRTDLKEEHKSSMMPGFLSEVNALSAVSSVVNKFNPFDLISDSEASQEETTKKQKVVQKEQGKPEGIIKPPLQQQPPKPIPKQQGPGRDPLQQDGTPKSISSQQPEKIKSQPPGTGKPIQGPTQTPQTDHAKLPLQRDASRPQTKQADIVRGESVKPSLPSPSKPPIQQPTPGKPPAQQPGHEKSQPGPAKPPAQPSGLTKPLAQQPGTVKPPVQPPGTTKPPAQPLGPAKPPAQQTGSEKPSSEQPGPKALAQPPGVGKTPAQQPGPAKPPTQQVGTPKPLAQQPGLQSPAKAPGPTKTPVQQPGPGKIPAQQAGPGKTSAQQTGPTKPPSQLPGPAKPPPQQPGPAKPPPQQPGSAKPPSQQPGSTKPPPQQPGPAKPSPQQPGSTKPPSQQPGSAKPSAQQPSPAKPSAQQSTKPVSQTGSGKPLQPPTVSPSAKQPPSQGLPKTICPLCNTTELLLHVPEKANFNTCTECQTTVCSLCGFNPNPHLTEVKEWLCLNCQMKRALGGDLAPVPSSPQPKLKTAPVTTTSAVSKSSPQPQQTSPKKDAAPKQDLSKAPEPKKPPPLVKQPTLHGSPSAKAKQPPEADSLSKPAPPKEPSVPSEQDKAPVADDKPKQPKMVKPTTDLVSSSSATTKPDIPSSKVQSQAEEKTTPPLKTDSAKPSQSFPPTGEKVSPFDSKAIPRPASDSKIISHPGPSSESKGQKQVDPVQKKEEPKKAQTKMSPKPDAKPMPKGSPTPPGPRPTAGQTVPTPQQSPKPQEQSRRFSLNLGSITDAPKSQPTTPQETVTGKLFGFGASIFSQASNLISTAGQPGPHSQSGPGAPMKQAPAPSQPPTSQGPPKSTGQAPPAPAKSIPVKKETKAPAAEKLEPKAEQAPTVKRTETEKKPPPIKDSKSLTAEPQKAVLPTKLEKSPKPESTCPLCKTELNIGSKDPPNFNTCTECKNQVCNLCGFNPTPHLTEIQEWLCLNCQTQRAISGQLGDIRKMPPAPSGPKASPMPVPTESSSQKTAVPPQVKLVKKQEQEVKTEAEKVILEKVKETLSMEKIPPMVTTDQKQEESKLEKDKASALQEKKPLPEEKKLIPEEEKIRSEEKKPLLEEKKPTPEDKKLLPEAKTSAPEEQKHDLLKSQVQIAEEKLEGRVAPKTVQEGKQPQTKMEGLPSGTPQSLPKEDDKTTKTIKEQPQPPCTAKPDQVEPGKEKTTKGSQTMTSSGAQKKVKRTLPNPPPEEISTGTQSTFSTMGTVSRRRICRTNTMARAKILQDIDRELDLVERESAKLRKKQAELDEEEKEIDAKLRYLEMGINRRKEALLKEREKRERAYLQGVAEDRDYMSDSEVSSTRPTRIESQHGIERPRTAPQTEFSQFIPPQTQTESQLVPPTSPYTQYQYSSPALPTQAPTSYTQQSHFEQQTLYHQQVSPYQTQPTFQAVATMSFTPQVQPTPTPQPSYQLPSQMMVIQQKPRQTTLYLEPKITSNYEVIRNQPLMIAPVSTDNTFAVSHLGSKYNSLDLRIGLEERSSMASSPISSISADSFYADIDHHTPRNYVLIDDIGEITKGTAALSTAFSLHEKDLSKTDRLLRTTETRRSQEVTDFLAPLQSSSRLHSYVKAEEDPMEDPYELKLLKHQIKQEFRRGTESLDHLAGLSHYYHADTSYRHFPKSEKYSISRLTLEKQAAKQLPAAILYQKQSKHKKSLIDPKMSKFSPIQESRDLEPDYSSYMTSSTSSIGGISSRARLLQDDITFGLRKNITDQQKFMGSSLGTGLGTLGNTIRSALQDEADKPYSSGSRSRPSSRPSSVYGLDLSIKRDSSSSSLRLKAQEAEALDVSFSHASSSARTKPTSLPISQSRGRIPIVAQNSEEESPLSPVGQPMGMARAAAGPLPPISADTRDQFGSSHSLPEVQQHMREESRTRGYDRDIAFIMDDFQHAMSDSEAYHLRREETDWFDKPRESRLENGHGLDRKLPERLVHSRPLSQHQEQIIQMNGKTMHYIFPHARIKITRDSKDHTVSGNGLGIRIVGGKEIPGHSGEIGAYIAKILPGGSAEQTGKLMEGMQVLEWNGIPLTSKTYEEVQSIISQQSGEAEICVRLDLNMLSDSENSQHLELHEPPKAVDKAKSPGVDPKQLAAELQKVSLQQSPLVLSSVVEKGSHVHSGPTSAGSSSVPSPGQPGSPSVSKKKHGSSKPTDGTKVVSHPITGEIQLQINYDLGNLIIHILQARNLVPRDNNGYSDPFVKVYLLPGRGAEYKRRTKHVQKSLNPEWNQTVIYKSISMEQLKKKTLEVTVWDYDRFSSNDFLGEVLIDLSSTSHLDNTPRWYPLKEQTESIDHGKSHSSQSSQQSPKPSVIKSRSHGIFPDPSKDMQVPTIEKSHSSPGSSKSSSEGHLRSHGPSRSQSKTSVTQTHLEDAGAAIAAAEAAVQQLRIQPSKRRK

Target ORF information:

RefSeq Version	XM_017012007.1
Organism	Homo sapiens(human)
Definition	Homo sapiens piccolo presynaptic cytomatrix protein (PCLO), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012007.1

ORF Insert Sequence:

ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA 
GCGGCTGGAG GAGGAGCTAG CGGGGCGGGG AGCCCCTCTC ACACCGCGAT CCCGGCCGGC 
ATGGAGGCGG ATTTGAGCCA GCTGAGCGAA GAGGAGAGGA GACAGATCGC CGCTGTCATG 
TCAAGGGCGC AGGGGCTGCC CAAGGGAAGC GTCCCCCCGG CCGCTGCGGA GTCGCCTTCC 
ATGCACAGGA AACAAGAGTT GGATAGTAGT CATCCTCCAA AGCAATCAGG AAGACCCCCG 
GACCCTGGGC GTCCAGCTCA ACCTGGTCTC AGTAAAAGTA GAACTACAGA CACTTTCAGG 
TCAGAGCAGA AATTGCCTGG GAGGAGTCCT TCCACTATTA GCTTGAAAGA ATCAAAGTCC 
AGAACTGATT TAAAGGAAGA GCACAAGTCT AGTATGATGC CTGGCTTCCT CTCAGAGGTT 
AACGCTTTAA GTGCTGTTTC CTCTGTTGTA AATAAATTCA ACCCTTTTGA TTTGATATCA 
GACTCTGAGG CATCCCAGGA AGAAACCACC AAGAAACAAA AAGTGGTTCA GAAGGAGCAA 
GGAAAACCTG AAGGAATCAT AAAACCTCCT TTACAACAAC AGCCACCCAA GCCGATTCCT 
AAGCAGCAAG GACCTGGTAG GGATCCGCTT CAGCAGGATG GCACTCCCAA ATCAATATCT 
TCTCAACAAC CAGAAAAAAT TAAATCACAA CCTCCAGGTA CAGGAAAGCC AATTCAGGGT 
CCTACCCAGA CTCCTCAGAC AGACCATGCA AAATTGCCAC TTCAACGAGA TGCATCCAGG 
CCTCAGACTA AACAGGCAGA CATAGTAAGG GGAGAATCAG TTAAACCCTC ACTGCCAAGC 
CCATCCAAAC CACCTATTCA GCAACCAACT CCTGGAAAAC CTCCAGCACA GCAGCCTGGA 
CATGAAAAAT CACAGCCTGG GCCTGCAAAG CCCCCAGCTC AGCCCTCAGG GCTAACAAAG 
CCATTGGCTC AACAACCAGG GACAGTGAAA CCCCCAGTCC AGCCACCAGG GACAACAAAG 
CCTCCAGCTC AGCCTCTTGG TCCTGCTAAG CCTCCAGCTC AGCAGACTGG GTCAGAGAAG 
CCTTCATCGG AGCAGCCTGG GCCAAAGGCT TTAGCTCAGC CTCCTGGAGT TGGAAAGACT 
CCAGCTCAAC AGCCAGGGCC AGCAAAGCCT CCAACCCAGC AGGTGGGGAC ACCAAAACCC 
CTAGCTCAAC AACCTGGGCT ACAGTCTCCA GCTAAGGCAC CTGGGCCTAC AAAGACTCCA 
GTTCAGCAGC CTGGGCCAGG AAAGATTCCA GCTCAACAGG CAGGACCCGG AAAGACTTCT 
GCCCAGCAGA CTGGCCCAAC AAAGCCTCCT TCACAACTGC CTGGCCCAGC AAAGCCCCCA 
CCTCAACAGC CTGGCCCAGC AAAGCCCCCA CCTCAACAGC CTGGCTCAGC AAAGCCCCCA 
TCTCAACAGC CTGGCTCAAC AAAACCCCCA CCTCAACAGC CTGGCCCAGC AAAGCCCTCA 
CCTCAACAGC CTGGCTCAAC AAAACCCCCA TCTCAACAGC CTGGCTCAGC AAAACCCTCA 
GCTCAACAGC CTAGCCCAGC AAAGCCCTCG GCTCAGCAAT CTACAAAACC AGTAAGCCAA 
ACAGGATCTG GAAAACCTCT GCAGCCACCA ACAGTGTCTC CATCTGCAAA ACAGCCTCCT 
TCACAAGGCC TCCCTAAAAC CATCTGTCCT CTTTGCAATA CCACTGAACT TCTGTTGCAT 
GTTCCAGAAA AGGCCAATTT TAACACATGC ACTGAGTGTC AAACCACTGT CTGTAGTCTC 
TGTGGTTTTA ATCCCAATCC TCATTTAACG GAGGTAAAAG AGTGGCTCTG TTTGAACTGT 
CAAATGAAAA GAGCTCTAGG CGGGGATCTG GCTCCAGTTC CGTCATCACC CCAGCCCAAA 
CTGAAGACTG CACCTGTTAC CACTACATCA GCAGTGAGCA AATCATCCCC ACAGCCACAG 
CAGACTTCCC CAAAGAAGGA TGCTGCACCA AAACAGGATC TCTCCAAGGC ACCTGAGCCT 
AAAAAGCCAC CACCACTAGT GAAACAACCA ACCCTTCATG GCTCTCCTTC AGCCAAGGCC 
AAGCAGCCCC CTGAGGCAGA TTCTTTGTCC AAGCCAGCCC CTCCCAAAGA ACCTTCTGTC 
CCATCTGAGC AGGACAAGGC CCCTGTTGCT GATGATAAAC CAAAGCAGCC CAAGATGGTA 
AAGCCAACCA CTGACCTTGT ATCTTCATCA TCAGCAACAA CAAAACCTGA TATTCCAAGC 
TCCAAAGTAC AGTCACAAGC TGAAGAGAAA ACAACCCCTC CTCTAAAAAC AGACTCTGCC 
AAACCCTCAC AGAGTTTTCC ACCAACAGGG GAAAAAGTCA GCCCATTTGA TTCTAAAGCC 
ATACCTCGAC CTGCATCAGA TTCAAAAATT ATTTCACATC CTGGTCCCAG TTCAGAGAGC 
AAAGGTCAAA AACAAGTTGA CCCCGTACAA AAGAAGGAAG AACCCAAGAA AGCACAAACC 
AAAATGAGTC CTAAACCAGA TGCCAAGCCA ATGCCAAAAG GGTCACCAAC ACCCCCTGGC 
CCACGACCTA CCGCTGGCCA AACTGTCCCC ACACCTCAAC AGTCCCCAAA GCCTCAGGAG 
CAGTCAAGGC GTTTCAGTCT GAATCTGGGA AGTATTACTG ATGCCCCCAA ATCACAGCCT 
ACAACTCCTC AAGAGACCGT GACTGGGAAA CTCTTTGGGT TTGGAGCATC AATCTTCAGC 
CAGGCATCAA ATTTAATTTC CACTGCAGGC CAACCTGGAC CTCATTCACA AAGTGGACCA 
GGGGCCCCAA TGAAACAAGC CCCTGCCCCT TCACAGCCAC CTACTTCACA AGGGCCACCC 
AAATCCACAG GTCAAGCACC ACCAGCACCT GCAAAAAGTA TACCTGTGAA AAAGGAAACA 
AAAGCCCCAG CAGCTGAAAA ATTAGAGCCC AAAGCTGAAC AAGCTCCAAC AGTAAAAAGA 
ACAGAAACAG AAAAAAAGCC ACCACCTATT AAGGATAGCA AATCTTTAAC AGCTGAGCCT 
CAAAAGGCTG TCCTTCCCAC AAAACTGGAG AAATCGCCCA AACCAGAATC AACCTGTCCT 
CTCTGCAAAA CTGAACTCAA CATAGGTTCT AAGGATCCTC CTAACTTCAA TACTTGCACT 
GAATGCAAGA ATCAAGTGTG TAATCTCTGT GGATTTAACC CTACACCACA TTTGACTGAG 
ATTCAAGAAT GGCTTTGTTT AAATTGCCAA ACCCAGAGAG CAATATCAGG ACAGCTTGGA 
GACATACGCA AAATGCCACC TGCACCATCA GGACCCAAAG CATCTCCTAT GCCTGTTCCT 
ACAGAATCAT CATCTCAGAA AACAGCAGTG CCTCCCCAAG TAAAATTAGT GAAAAAGCAA 
GAACAAGAAG TAAAAACGGA AGCTGAAAAA GTCATTCTGG AAAAAGTAAA GGAAACACTA 
TCAATGGAAA AAATTCCTCC TATGGTAACC ACAGATCAAA AACAAGAAGA GAGTAAACTA 
GAGAAAGACA AAGCTTCAGC TCTTCAAGAA AAAAAGCCAC TCCCTGAAGA AAAAAAACTA 
ATCCCTGAAG AAGAAAAGAT ACGTTCTGAA GAAAAAAAGC CACTCCTAGA AGAAAAAAAG 
CCAACCCCTG AAGACAAAAA GCTACTCCCA GAGGCAAAAA CATCAGCCCC AGAAGAACAG 
AAACATGACT TACTTAAATC TCAAGTACAA ATTGCTGAAG AAAAGCTTGA AGGCAGAGTG 
GCTCCAAAGA CAGTGCAAGA AGGGAAACAA CCACAGACCA AGATGGAAGG TTTACCATCT 
GGCACACCTC AGAGTTTACC TAAAGAAGAT GATAAGACAA CCAAAACAAT AAAAGAACAG 
CCACAGCCAC CATGCACAGC AAAACCTGAT CAGGTGGAAC CTGGGAAAGA AAAAACAACT 
AAAGGTTCTC AAACCATGAC ATCCTCTGGA GCCCAGAAAA AAGTTAAAAG AACTCTGCCA 
AATCCACCTC CTGAGGAGAT TTCCACAGGA ACTCAATCCA CATTCAGCAC AATGGGCACA 
GTTTCCAGGA GAAGGATCTG CAGAACCAAC ACAATGGCAC GAGCCAAGAT TCTCCAGGAC 
ATAGACAGAG AGCTTGATCT TGTGGAAAGG GAGTCTGCAA AACTTCGAAA GAAACAAGCA 
GAGCTTGATG AAGAAGAAAA GGAGATTGAT GCTAAGCTAC GATACCTGGA AATGGGAATT 
AACAGGAGGA AAGAGGCCCT ATTAAAGGAG AGAGAAAAGA GAGAACGAGC CTACCTCCAG 
GGAGTAGCTG AGGATCGTGA TTACATGTCT GACAGTGAAG TGAGTAGCAC AAGACCAACC 
CGAATAGAAA GTCAGCATGG CATTGAGCGA CCAAGAACTG CTCCCCAAAC TGAATTCAGC 
CAGTTTATAC CACCACAAAC CCAAACAGAA TCTCAACTAG TTCCTCCGAC AAGTCCTTAC 
ACACAATACC AGTACTCTTC CCCTGCTCTT CCTACCCAAG CACCCACCTC ATACACTCAA 
CAGTCTCATT TTGAGCAACA AACTTTGTAC CATCAGCAAG TTTCACCTTA TCAGACTCAG 
CCAACATTCC AAGCTGTGGC AACAATGTCC TTCACACCTC AAGTTCAACC TACACCAACC 
CCACAGCCTT CTTATCAGTT ACCTTCACAG ATGATGGTGA TACAACAGAA GCCACGGCAA 
ACTACATTAT ATTTGGAGCC CAAGATAACC TCAAACTATG AAGTGATTCG CAACCAACCC 
CTTATGATAG CACCTGTTTC TACGGATAAC ACATTTGCTG TTTCCCATCT TGGTAGTAAG 
TACAATAGTT TAGACTTGAG AATAGGTTTG GAGGAAAGAA GTAGCATGGC AAGCAGTCCA 
ATATCAAGCA TATCTGCAGA TTCTTTCTAT GCAGATATTG ATCACCATAC TCCACGAAAT 
TATGTCCTAA TTGACGACAT TGGAGAGATC ACCAAAGGAA CAGCGGCATT AAGCACCGCA 
TTTAGCCTTC ATGAAAAGGA TCTGTCAAAA ACAGACCGTC TCCTTCGAAC CACTGAGACA 
CGCCGGTCTC AAGAAGTGAC AGATTTCCTA GCACCTTTAC AGTCTTCCTC TAGATTGCAT 
AGTTATGTGA AGGCGGAGGA AGACCCAATG GAGGATCCTT ACGAGTTAAA GCTTCTGAAA 
CATCAGATTA AACAGGAATT TCGTAGAGGG ACAGAGAGCT TAGATCACCT TGCTGGTCTT 
TCTCATTATT ACCATGCTGA TACTAGCTAC AGACATTTTC CAAAATCTGA GAAGTATAGC 
ATCAGTAGAC TCACACTTGA AAAACAAGCA GCAAAACAAC TGCCAGCAGC CATACTTTAT 
CAAAAGCAGT CAAAGCATAA GAAATCACTA ATTGACCCTA AAATGTCAAA ATTTTCACCT 
ATTCAAGAAA GTAGAGACCT TGAACCTGAT TATTCAAGCT ATATGACTTC TAGCACTTCA 
TCTATTGGTG GCATTTCCTC CAGGGCAAGG CTCCTTCAAG ATGACATCAC TTTTGGCCTC 
AGAAAAAATA TTACAGACCA ACAAAAATTT ATGGGATCTT CTCTTGGCAC AGGACTGGGC 
ACATTAGGAA ATACCATACG CTCAGCTCTG CAGGATGAAG CGGATAAGCC ATACAGTAGT 
GGCAGCAGGT CCAGACCTTC CTCCAGACCT TCCTCTGTCT ATGGGCTTGA TTTATCAATT 
AAAAGGGATT CTTCTAGCTC TTCCCTAAGA CTGAAAGCTC AAGAGGCTGA AGCTCTAGAT 
GTTTCCTTTA GTCATGCATC ATCCTCTGCC AGAACTAAGC CGACCAGTTT GCCAATTAGT 
CAAAGTAGAG GAAGAATACC AATTGTGGCC CAGAATTCTG AAGAAGAAAG CCCACTCAGT 
CCTGTTGGCC AGCCAATGGG AATGGCCAGG GCTGCAGCTG GACCCCTGCC ACCAATATCT 
GCAGACACCA GGGATCAGTT TGGATCAAGC CACTCATTGC CTGAAGTTCA GCAACACATG 
AGGGAAGAAT CACGGACTCG AGGCTATGAC CGTGACATAG CATTCATCAT GGATGACTTC 
CAACATGCCA TGTCAGACAG TGAAGCCTAT CATCTGCGTC GTGAGGAAAC AGATTGGTTT 
GATAAACCCA GGGAGTCTCG TTTGGAAAAT GGACATGGTC TGGACCGAAA ACTGCCGGAA 
AGATTGGTCC ACTCTAGACC ACTCAGTCAA CATCAAGAGC AAATTATACA GATGAACGGG 
AAAACTATGC ACTACATCTT TCCTCACGCA AGGATAAAAA TAACAAGAGA CTCAAAGGAT 
CACACAGTTT CAGGTAATGG ATTAGGAATT AGAATTGTGG GTGGTAAAGA AATCCCGGGA 
CATAGTGGAG AAATTGGAGC CTATATTGCC AAGATTCTTC CTGGGGGAAG TGCGGAACAG 
ACGGGGAAGC TTATGGAAGG GATGCAAGTA TTGGAATGGA ATGGAATTCC CTTGACTTCT 
AAAACATATG AAGAAGTTCA GAGTATCATT AGTCAGCAAA GTGGGGAAGC AGAAATATGT 
GTAAGACTGG ACCTCAATAT GCTATCAGAT TCTGAAAATT CCCAGCATCT GGAACTTCAT 
GAGCCACCAA AAGCTGTGGA TAAGGCGAAA TCCCCAGGGG TTGATCCTAA GCAGTTGGCA 
GCAGAACTCC AGAAGGTTTC ACTACAGCAG TCACCGCTGG TTCTGTCATC AGTTGTTGAA 
AAAGGATCTC ATGTTCATTC AGGTCCTACA TCAGCAGGAT CCAGTTCCGT TCCCAGCCCT 
GGGCAACCAG GGTCCCCCTC AGTGAGCAAA AAGAAGCACG GCAGCAGCAA GCCTACCGAT 
GGAACAAAGG TTGTCTCTCA TCCAATTACA GGAGAAATTC AGCTTCAAAT TAACTATGAT 
CTTGGAAATC TCATAATACA TATTCTCCAA GCAAGAAATC TTGTTCCTCG AGACAACAAT 
GGTTATTCTG ACCCTTTTGT GAAAGTGTAC CTTCTTCCAG GGAGAGGTGC TGAGTACAAG 
AGAAGGACTA AACATGTCCA GAAAAGTCTT AATCCTGAGT GGAATCAAAC AGTAATTTAT 
AAAAGTATTT CCATGGAACA GCTCAAGAAG AAAACACTGG AGGTGACAGT TTGGGATTAT 
GATAGATTTT CATCCAACGA CTTCCTTGGG GAGGTATTGA TTGATTTATC TAGCACATCT 
CACCTCGATA ACACTCCAAG GTGGTATCCT CTCAAAGAAC AGACTGAAAG CATTGATCAT 
GGCAAGTCTC ATTCCAGTCA GAGCAGCCAG CAGTCCCCAA AGCCATCTGT TATCAAAAGC 
AGAAGCCATG GTATCTTCCC TGACCCATCA AAGGACATGC AGGTTCCCAC CATTGAGAAA 
TCCCATAGTA GTCCTGGTAG CTCAAAATCA TCATCAGAAG GCCATCTCCG TTCTCATGGA 
CCATCTCGCA GTCAAAGCAA AACCAGCGTC ACTCAGACCC ACCTGGAAGA TGCAGGGGCT 
GCCATAGCTG CTGCCGAAGC TGCCGTGCAA CAACTCCGCA TTCAACCAAG TAAAAGACGC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-Wide Significance for PCLO as a Gene for Major Depressive Disorder.
Twin research and human genetics : the official journal of the International Society for Twin Studies20(4)267-270(2017 Aug)
Mbarek H,Milaneschi Y,Hottenga JJ,Ligthart L,de Geus EJC,Ehli EA,Willemsen G,Davies GE,Smit JH,Boomsma DI,Penninx BWJH

Piccolo mediates EGFR signaling and acts as a prognostic biomarker in esophageal squamous cell carcinoma.
Oncogene36(27)3890-3902(2017 Jul)
Zhang W,Hong R,Xue L,Ou Y,Liu X,Zhao Z,Xiao W,Dong D,Dong L,Fu M,Ma L,Lu N,Chen H,Song Y,Zhan Q

A 7q21.11 microdeletion presenting with apparent intellectual disability without epilepsy.
American journal of medical genetics. Part A173(4)1128-1130(2017 04)
Siddique A,Willoughby J,,McNeill A

Quantitative proteomics reveals Piccolo as a candidate serological correlate of recovery from Guillain-Barr? syndrome.
Oncotarget7(46)74582-74591(2016 11)
Mateos-Hern?ndez L,Villar M,Doncel-P?rez E,Trevisan-Herraz M,Garc?a-Forcada ?,Ganuza FR,V?zquez J,de la Fuente J

GWAS-identified risk variants for major depressive disorder: Preliminary support for an association with late-life depressive symptoms and brain structural alterations.
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology26(1)113-25(2016 Jan)
Ryan J,Artero S,Carri?re I,Maller JJ,Meslin C,Ritchie K,Ancelin ML

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PCLO cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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