HTATSF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HTATSF1
Entrez Gene ID	27336
Full Name	HIV-1 Tat specific factor 1
Synonyms	TAT-SF1,TATSF1,dJ196E23.2
General protein information	Preferred Names HIV-1 Tat specific factor 1 Names HIV Tat-specific factor 1 HIV TAT specific factor 1 cofactor required for Tat activation of HIV-1 transcription
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq26.3
Summary	The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Sep 2009].
Disorder MIM:	300346

mRNA and Protein(s)

mRNA	Protein	Name
NM_014500.4	NP_055315.2	HIV Tat-specific factor 1
XM_005262404.3	XP_005262461.1	HIV Tat-specific factor 1 isoform X1
XM_005262404.4	XP_005262461.1	HIV Tat-specific factor 1 isoform X1
NM_014500.5	NP_055315.2	HIV Tat-specific factor 1
NM_001163280.1	NP_001156752.1	HIV Tat-specific factor 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HTATSF1	NP_001156752.1
Mus musculus (house mouse)	Htatsf1	NP_083647.1
Rattus norvegicus (Norway rat)	Htatsf1	NP_001101729.1
Gallus gallus (chicken)	HTATSF1	XP_001233032.1
Xenopus tropicalis (tropical clawed frog)	htatsf1	XP_002932639.2
Pan troglodytes (chimpanzee)	HTATSF1	XP_003317777.1
Canis lupus familiaris (dog)	HTATSF1	XP_538183.3
Danio rerio (zebrafish)	htatsf1	NP_957497.1
	HTATSF1	XP_001082302.2
Bos taurus (cattle)	HTATSF1	NP_001193343.1

Gene Ontology
Bibliography

Related articles in PubMed

Impact of sustained RNAi-mediated suppression of cellular cofactor Tat-SF1 on HIV-1 replication in CD4+ T cells.
Green VA, Arbuthnot P, Weinberg MS
Virology journal9272(2012 Nov)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Identification of Tat-SF1 cellular targets by exon array analysis reveals dual roles in transcription and splicing.
Miller HB, Robinson TJ, Gord?n R, Hartemink AJ, Garcia-Blanco MA
RNA (New York, N.Y.)17(4)665-74(2011 Apr)

DSIF, the Paf1 complex, and Tat-SF1 have nonredundant, cooperative roles in RNA polymerase II elongation.
Chen Y, Yamaguchi Y, Tsugeno Y, Yamamoto J, Yamada T, Nakamura M, Hisatake K, Handa H
Genes & development23(23)2765-77(2009 Dec)

Tat-SF1 is not required for Tat transactivation but does regulate the relative levels of unspliced and spliced HIV-1 RNAs.
Miller HB, Saunders KO, Tomaras GD, Garcia-Blanco MA
PloS one4(5)e5710(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study validates Tat-SF1 as an HDF in CD4+ T cell-derived SupT1 cells.
Title: Impact of sustained RNAi-mediated suppression of cellular cofactor Tat-SF1 on HIV-1 replication in CD4+ T cells.

these findings suggest that Tat-SF1 functions independently in transcription and splicing of cellular genes.
Title: Identification of Tat-SF1 cellular targets by exon array analysis reveals dual roles in transcription and splicing.

Tat-SF1 is not required for regulating HIV-1 transcription, but is required for maintaining the ratios of different classes of HIV-1 transcripts.
Title: Tat-SF1 is not required for Tat transactivation but does regulate the relative levels of unspliced and spliced HIV-1 RNAs.

Intracellular HIV-1 Tat protein represses constitutive LMP2 transcription increasing proteasome activity by interfering with the binding of IRF-1 to STAT1
Title: Intracellular HIV-1 Tat protein represses constitutive LMP2 transcription increasing proteasome activity by interfering with the binding of IRF-1 to STAT1.

phosphorylated by P-TEFb kinase during HIV-1 transcription in Tat/TAR dependent manner
Title: Coordination of transcription factor phosphorylation and histone methylation by the P-TEFb kinase during human immunodeficiency virus type 1 transcription.

The following HTATSF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HTATSF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18558	NM_001163280.1 Latest version!	Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu18558	NM_014500.5 Latest version!	Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu18558	XM_005262404.4 Latest version!	Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OHu18558	XM_005262404.3	Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu18558	NM_014500.4	Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HTATSF1 ( NM_014500.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055315.2 Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18558
Clone ID Related Accession (Same CDS sequence)	NM_014500.4 , XM_005262404.3 , XM_005262404.4 , NM_014500.5 , NM_001163280.1
Accession Version	NM_014500.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	HIV Tat-specific factor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC009896.2 and BU633070.1. This sequence is a reference standard in the RefSeqGene project. On Jul 15, 2009 this sequence version replaced NM_014500.3. Transcript Variant: This variant (2) is missing a 5' non-coding exon, and therefore, has a different 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009896.2, SRR1803616.67990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGGCA CCAACTTGGA TGGGAACGAT GAGTTTGATG AGCAGTTGCG AATGCAAGAA 
TTGTACGGAG ACGGCAAGGA TGGTGACACC CAGACCGATG CCGGCGGAGA ACCCGATTCT 
CTCGGGCAGC AGCCGACGGA CACTCCCTAC GAGTGGGACC TGGACAAAAA GGCTTGGTTC 
CCCAAGATTA CTGAAGATTT CATTGCTACA TATCAGGCCA ATTATGGCTT CTCTAACGAT 
GGCGCATCTA GTTCTACCGC AAATGTTGAA GATGTCCATG CTAGGACTGC AGAGGAACCT 
CCACAAGAAA AAGCCCCGGA ACCCACTGAT GCCAGAAAGA AGGGAGAAAA AAGAAAGGCT 
GAGTCAGGAT GGTTTCATGT TGAAGAAGAC AGAAATACAA ATGTATACGT GTCTGGTTTG 
CCTCCAGATA TTACAGTGGA TGAATTTATA CAACTTATGT CCAAGTTTGG CATTATTATG 
AGAGATCCTC AGACAGAAGA ATTTAAGGTC AAACTTTACA AAGATAATCA AGGAAATCTT 
AAAGGAGACG GTCTTTGCTG TTATTTGAAA AGAGAATCTG TGGAACTTGC ATTAAAACTT 
TTGGATGAAG ATGAAATTAG AGGCTACAAA TTACATGTTG AGGTGGCAAA GTTTCAACTG 
AAGGGAGAAT ATGATGCCTC AAAGAAGAAG AAGAAGTGCA AAGACTATAA GAAGAAGCTG 
TCTATGCAAC AAAAGCAGTT GGATTGGAGA CCTGAGAGGC GAGCCGGACC ATCCCGGATG 
CGCCATGAGC GAGTTGTCAT CATCAAGAAT ATGTTTCATC CTATGGATTT TGAGGATGAT 
CCGTTGGTGC TGAATGAGAT CAGAGAAGAC CTTCGAGTAG AGTGTTCGAA GTTTGGACAA 
ATTAGGAAAC TCCTTCTCTT TGATAGGCAC CCAGATGGTG TGGCCTCTGT GTCCTTTCGG 
GATCCAGAGG AAGCTGATTA TTGTATTCAG ACTCTCGATG GAAGATGGTT TGGTGGCCGT 
CAAATCACTG CCCAGGCATG GGATGGGACT ACAGATTATC AGGTGGAGGA AACCTCAAGA 
GAAAGGGAGG AAAGGCTGAG AGGATGGGAG GCTTTCCTCA ATGCTCCTGA GGCCAACAGA 
GGCCTTAGGC GTTCAGATTC TGTCTCTGCT TCCGAAAGGG CAGGGCCTTC TAGAGCAAGG 
CATTTTTCAG AGCACCCCAG CACATCTAAA ATGAATGCTC AAGAAACTGC AACTGGAATG 
GCGTTTGAAG AACCTATAGA TGAGAAGAAG TTTGAAAAGA CAGAAGATGG GGGAGAATTT 
GAAGAAGGTG CTTCTGAAAA CAATGCTAAG GAAAGTAGCC CCGAAAAAGA GGCTGAAGAA 
GGCTGCCCTG AAAAAGAATC TGAAGAGGGC TGCCCCAAAA GAGGGTTTGA AGGCAGCTGC 
TCCCAAAAAG AGTCTGAAGA AGGCAATCCC GTAAGAGGAT CTGAAGAGGA TAGTCCTAAA 
AAAGAGTCTA AAAAGAAGAC ACTCAAAAAT GATTGTGAAG AGAATGGCCT TGCAAAGGAA 
TCTGAAGATG ACCTCAACAA GGAGTCTGAA GAGGAGGTTG GCCCCACAAA AGAGTCCGAA 
GAAGATGACT CAGAGAAAGA GTCTGATGAA GACTGCTCTG AAAAACAGTC TGAAGATGGC 
TCCGAAAGAG AATTTGAAGA AAATGGTCTC GAGAAAGATT TGGACGAGGA AGGTTCTGAA 
AAGGAGCTTC ATGAAAATGT TCTTGACAAA GAGTTAGAAG AAAATGACTC TGAAAACTCC 
GAATTTGAAG ATGACGGCTC TGAAAAAGTG TTAGATGAGG AAGGCTCTGA GAGAGAGTTT 
GACGAAGATT CAGATGAAAA GGAAGAAGAG GAGGATACAT ATGAAAAAGT ATTTGATGAT 
GAGTCTGATG AGAAAGAGGA TGAAGAATAT GCAGATGAAA AGGGGCTTGA AGCTGCTGAT 
AAAAAGGCGG AAGAAGGTGA TGCAGATGAA AAGCTGTTTG AAGAGTCAGA TGACAAGGAA 
GATGAAGATG CAGATGGAAA GGAAGTTGAA GATGCTGACG AAAAGTTGTT CGAAGATGAT 
GATTCCAATG AGAAGTTGTT TGATGAGGAG GAAGATTCCA GTGAGAAGTT GTTTGACGAT 
TCTGATGAGA GGGGGACTTT GGGTGGTTTT GGGAGTGTTG AAGAAGGGCC CCTATCCACT 
GGCAGCAGCT TTATTCTCAG TAGCGATGAT GATGACGATG ATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055315.2
CDS	174..2441
Translation	MSGTNLDGNDEFDEQLRMQELYGDGKDGDTQTDAGGEPDSLGQQPTDTPYEWDLDKKAWFPKITEDFIATYQANYGFSNDGASSSTANVEDVHARTAEEPPQEKAPEPTDARKKGEKRKAESGWFHVEEDRNTNVYVSGLPPDITVDEFIQLMSKFGIIMRDPQTEEFKVKLYKDNQGNLKGDGLCCYLKRESVELALKLLDEDEIRGYKLHVEVAKFQLKGEYDASKKKKKCKDYKKKLSMQQKQLDWRPERRAGPSRMRHERVVIIKNMFHPMDFEDDPLVLNEIREDLRVECSKFGQIRKLLLFDRHPDGVASVSFRDPEEADYCIQTLDGRWFGGRQITAQAWDGTTDYQVEETSREREERLRGWEAFLNAPEANRGLRRSDSVSASERAGPSRARHFSEHPSTSKMNAQETATGMAFEEPIDEKKFEKTEDGGEFEEGASENNAKESSPEKEAEEGCPEKESEEGCPKRGFEGSCSQKESEEGNPVRGSEEDSPKKESKKKTLKNDCEENGLAKESEDDLNKESEEEVGPTKESEEDDSEKESDEDCSEKQSEDGSEREFEENGLEKDLDEEGSEKELHENVLDKELEENDSENSEFEDDGSEKVLDEEGSEREFDEDSDEKEEEEDTYEKVFDDESDEKEDEEYADEKGLEAADKKAEEGDADEKLFEESDDKEDEDADGKEVEDADEKLFEDDDSNEKLFDEEEDSSEKLFDDSDERGTLGGFGSVEEGPLSTGSSFILSSDDDDDDI

Target ORF information:

RefSeq Version	NM_014500.4
Organism	Homo sapiens(human)
Definition	Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014500.4

ORF Insert Sequence:

ATGAGCGGCA CCAACTTGGA TGGGAACGAT GAGTTTGATG AGCAGTTGCG AATGCAAGAA 
TTGTACGGAG ACGGCAAGGA TGGTGACACC CAGACCGATG CCGGCGGAGA ACCCGATTCT 
CTCGGGCAGC AGCCGACGGA CACTCCCTAC GAGTGGGACC TGGACAAAAA GGCTTGGTTC 
CCCAAGATTA CTGAAGATTT CATTGCTACA TATCAGGCCA ATTATGGCTT CTCTAACGAT 
GGCGCATCTA GTTCTACCGC AAATGTTGAA GATGTCCATG CTAGGACTGC AGAGGAACCT 
CCACAAGAAA AAGCCCCGGA ACCCACTGAT GCCAGAAAGA AGGGAGAAAA AAGAAAGGCT 
GAGTCAGGAT GGTTTCATGT TGAAGAAGAC AGAAATACAA ATGTATACGT GTCTGGTTTG 
CCTCCAGATA TTACAGTGGA TGAATTTATA CAACTTATGT CCAAGTTTGG CATTATTATG 
AGAGATCCTC AGACAGAAGA ATTTAAGGTC AAACTTTACA AAGATAATCA AGGAAATCTT 
AAAGGAGACG GTCTTTGCTG TTATTTGAAA AGAGAATCTG TGGAACTTGC ATTAAAACTT 
TTGGATGAAG ATGAAATTAG AGGCTACAAA TTACATGTTG AGGTGGCAAA GTTTCAACTG 
AAGGGAGAAT ATGATGCCTC AAAGAAGAAG AAGAAGTGCA AAGACTATAA GAAGAAGCTG 
TCTATGCAAC AAAAGCAGTT GGATTGGAGA CCTGAGAGGC GAGCCGGACC ATCCCGGATG 
CGCCATGAGC GAGTTGTCAT CATCAAGAAT ATGTTTCATC CTATGGATTT TGAGGATGAT 
CCGTTGGTGC TGAATGAGAT CAGAGAAGAC CTTCGAGTAG AGTGTTCGAA GTTTGGACAA 
ATTAGGAAAC TCCTTCTCTT TGATAGGCAC CCAGATGGTG TGGCCTCTGT GTCCTTTCGG 
GATCCAGAGG AAGCTGATTA TTGTATTCAG ACTCTCGATG GAAGATGGTT TGGTGGCCGT 
CAAATCACTG CCCAGGCATG GGATGGGACT ACAGATTATC AGGTGGAGGA AACCTCAAGA 
GAAAGGGAGG AAAGGCTGAG AGGATGGGAG GCTTTCCTCA ATGCTCCTGA GGCCAACAGA 
GGCCTTAGGC GTTCAGATTC TGTCTCTGCT TCCGAAAGGG CAGGGCCTTC TAGAGCAAGG 
CATTTTTCAG AGCACCCCAG CACATCTAAA ATGAATGCTC AAGAAACTGC AACTGGAATG 
GCGTTTGAAG AACCTATAGA TGAGAAGAAG TTTGAAAAGA CAGAAGATGG GGGAGAATTT 
GAAGAAGGTG CTTCTGAAAA CAATGCTAAG GAAAGTAGCC CCGAAAAAGA GGCTGAAGAA 
GGCTGCCCTG AAAAAGAATC TGAAGAGGGC TGCCCCAAAA GAGGGTTTGA AGGCAGCTGC 
TCCCAAAAAG AGTCTGAAGA AGGCAATCCC GTAAGAGGAT CTGAAGAGGA TAGTCCTAAA 
AAAGAGTCTA AAAAGAAGAC ACTCAAAAAT GATTGTGAAG AGAATGGCCT TGCAAAGGAA 
TCTGAAGATG ACCTCAACAA GGAGTCTGAA GAGGAGGTTG GCCCCACAAA AGAGTCCGAA 
GAAGATGACT CAGAGAAAGA GTCTGATGAA GACTGCTCTG AAAAACAGTC TGAAGATGGC 
TCCGAAAGAG AATTTGAAGA AAATGGTCTC GAGAAAGATT TGGACGAGGA AGGTTCTGAA 
AAGGAGCTTC ATGAAAATGT TCTTGACAAA GAGTTAGAAG AAAATGACTC TGAAAACTCC 
GAATTTGAAG ATGACGGCTC TGAAAAAGTG TTAGATGAGG AAGGCTCTGA GAGAGAGTTT 
GACGAAGATT CAGATGAAAA GGAAGAAGAG GAGGATACAT ATGAAAAAGT ATTTGATGAT 
GAGTCTGATG AGAAAGAGGA TGAAGAATAT GCAGATGAAA AGGGGCTTGA AGCTGCTGAT 
AAAAAGGCGG AAGAAGGTGA TGCAGATGAA AAGCTGTTTG AAGAGTCAGA TGACAAGGAA 
GATGAAGATG CAGATGGAAA GGAAGTTGAA GATGCTGACG AAAAGTTGTT CGAAGATGAT 
GATTCCAATG AGAAGTTGTT TGATGAGGAG GAAGATTCCA GTGAGAAGTT GTTTGACGAT 
TCTGATGAGA GGGGGACTTT GGGTGGTTTT GGGAGTGTTG AAGAAGGGCC CCTATCCACT 
GGCAGCAGCT TTATTCTCAG TAGCGATGAT GATGACGATG ATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HTATSF1 ( NM_014500.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055315.2 Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18558
Clone ID Related Accession (Same CDS sequence)	NM_014500.4 , XM_005262404.3 , XM_005262404.4 , NM_014500.5 , NM_001163280.1
Accession Version	NM_014500.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	HIV Tat-specific factor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC009896.2 and BU633070.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_014500.4. Transcript Variant: This variant (2) is missing a 5' non-coding exon, and therefore, has a different 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.836266.1, SRR1660807.5254.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000218364.5/ ENSP00000218364.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCGGCA CCAACTTGGA TGGGAACGAT GAGTTTGATG AGCAGTTGCG AATGCAAGAA 
TTGTACGGAG ACGGCAAGGA TGGTGACACC CAGACCGATG CCGGCGGAGA ACCCGATTCT 
CTCGGGCAGC AGCCGACGGA CACTCCCTAC GAGTGGGACC TGGACAAAAA GGCTTGGTTC 
CCCAAGATTA CTGAAGATTT CATTGCTACA TATCAGGCCA ATTATGGCTT CTCTAACGAT 
GGCGCATCTA GTTCTACCGC AAATGTTGAA GATGTCCATG CTAGGACTGC AGAGGAACCT 
CCACAAGAAA AAGCCCCGGA ACCCACTGAT GCCAGAAAGA AGGGAGAAAA AAGAAAGGCT 
GAGTCAGGAT GGTTTCATGT TGAAGAAGAC AGAAATACAA ATGTATACGT GTCTGGTTTG 
CCTCCAGATA TTACAGTGGA TGAATTTATA CAACTTATGT CCAAGTTTGG CATTATTATG 
AGAGATCCTC AGACAGAAGA ATTTAAGGTC AAACTTTACA AAGATAATCA AGGAAATCTT 
AAAGGAGACG GTCTTTGCTG TTATTTGAAA AGAGAATCTG TGGAACTTGC ATTAAAACTT 
TTGGATGAAG ATGAAATTAG AGGCTACAAA TTACATGTTG AGGTGGCAAA GTTTCAACTG 
AAGGGAGAAT ATGATGCCTC AAAGAAGAAG AAGAAGTGCA AAGACTATAA GAAGAAGCTG 
TCTATGCAAC AAAAGCAGTT GGATTGGAGA CCTGAGAGGC GAGCCGGACC ATCCCGGATG 
CGCCATGAGC GAGTTGTCAT CATCAAGAAT ATGTTTCATC CTATGGATTT TGAGGATGAT 
CCGTTGGTGC TGAATGAGAT CAGAGAAGAC CTTCGAGTAG AGTGTTCGAA GTTTGGACAA 
ATTAGGAAAC TCCTTCTCTT TGATAGGCAC CCAGATGGTG TGGCCTCTGT GTCCTTTCGG 
GATCCAGAGG AAGCTGATTA TTGTATTCAG ACTCTCGATG GAAGATGGTT TGGTGGCCGT 
CAAATCACTG CCCAGGCATG GGATGGGACT ACAGATTATC AGGTGGAGGA AACCTCAAGA 
GAAAGGGAGG AAAGGCTGAG AGGATGGGAG GCTTTCCTCA ATGCTCCTGA GGCCAACAGA 
GGCCTTAGGC GTTCAGATTC TGTCTCTGCT TCCGAAAGGG CAGGGCCTTC TAGAGCAAGG 
CATTTTTCAG AGCACCCCAG CACATCTAAA ATGAATGCTC AAGAAACTGC AACTGGAATG 
GCGTTTGAAG AACCTATAGA TGAGAAGAAG TTTGAAAAGA CAGAAGATGG GGGAGAATTT 
GAAGAAGGTG CTTCTGAAAA CAATGCTAAG GAAAGTAGCC CCGAAAAAGA GGCTGAAGAA 
GGCTGCCCTG AAAAAGAATC TGAAGAGGGC TGCCCCAAAA GAGGGTTTGA AGGCAGCTGC 
TCCCAAAAAG AGTCTGAAGA AGGCAATCCC GTAAGAGGAT CTGAAGAGGA TAGTCCTAAA 
AAAGAGTCTA AAAAGAAGAC ACTCAAAAAT GATTGTGAAG AGAATGGCCT TGCAAAGGAA 
TCTGAAGATG ACCTCAACAA GGAGTCTGAA GAGGAGGTTG GCCCCACAAA AGAGTCCGAA 
GAAGATGACT CAGAGAAAGA GTCTGATGAA GACTGCTCTG AAAAACAGTC TGAAGATGGC 
TCCGAAAGAG AATTTGAAGA AAATGGTCTC GAGAAAGATT TGGACGAGGA AGGTTCTGAA 
AAGGAGCTTC ATGAAAATGT TCTTGACAAA GAGTTAGAAG AAAATGACTC TGAAAACTCC 
GAATTTGAAG ATGACGGCTC TGAAAAAGTG TTAGATGAGG AAGGCTCTGA GAGAGAGTTT 
GACGAAGATT CAGATGAAAA GGAAGAAGAG GAGGATACAT ATGAAAAAGT ATTTGATGAT 
GAGTCTGATG AGAAAGAGGA TGAAGAATAT GCAGATGAAA AGGGGCTTGA AGCTGCTGAT 
AAAAAGGCGG AAGAAGGTGA TGCAGATGAA AAGCTGTTTG AAGAGTCAGA TGACAAGGAA 
GATGAAGATG CAGATGGAAA GGAAGTTGAA GATGCTGACG AAAAGTTGTT CGAAGATGAT 
GATTCCAATG AGAAGTTGTT TGATGAGGAG GAAGATTCCA GTGAGAAGTT GTTTGACGAT 
TCTGATGAGA GGGGGACTTT GGGTGGTTTT GGGAGTGTTG AAGAAGGGCC CCTATCCACT 
GGCAGCAGCT TTATTCTCAG TAGCGATGAT GATGACGATG ATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055315.2
CDS	94..2361
Translation	MSGTNLDGNDEFDEQLRMQELYGDGKDGDTQTDAGGEPDSLGQQPTDTPYEWDLDKKAWFPKITEDFIATYQANYGFSNDGASSSTANVEDVHARTAEEPPQEKAPEPTDARKKGEKRKAESGWFHVEEDRNTNVYVSGLPPDITVDEFIQLMSKFGIIMRDPQTEEFKVKLYKDNQGNLKGDGLCCYLKRESVELALKLLDEDEIRGYKLHVEVAKFQLKGEYDASKKKKKCKDYKKKLSMQQKQLDWRPERRAGPSRMRHERVVIIKNMFHPMDFEDDPLVLNEIREDLRVECSKFGQIRKLLLFDRHPDGVASVSFRDPEEADYCIQTLDGRWFGGRQITAQAWDGTTDYQVEETSREREERLRGWEAFLNAPEANRGLRRSDSVSASERAGPSRARHFSEHPSTSKMNAQETATGMAFEEPIDEKKFEKTEDGGEFEEGASENNAKESSPEKEAEEGCPEKESEEGCPKRGFEGSCSQKESEEGNPVRGSEEDSPKKESKKKTLKNDCEENGLAKESEDDLNKESEEEVGPTKESEEDDSEKESDEDCSEKQSEDGSEREFEENGLEKDLDEEGSEKELHENVLDKELEENDSENSEFEDDGSEKVLDEEGSEREFDEDSDEKEEEEDTYEKVFDDESDEKEDEEYADEKGLEAADKKAEEGDADEKLFEESDDKEDEDADGKEVEDADEKLFEDDDSNEKLFDEEEDSSEKLFDDSDERGTLGGFGSVEEGPLSTGSSFILSSDDDDDDI

Target ORF information:

RefSeq Version	NM_014500.5
Organism	Homo sapiens(human)
Definition	Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014500.5

ORF Insert Sequence:

ATGAGCGGCA CCAACTTGGA TGGGAACGAT GAGTTTGATG AGCAGTTGCG AATGCAAGAA 
TTGTACGGAG ACGGCAAGGA TGGTGACACC CAGACCGATG CCGGCGGAGA ACCCGATTCT 
CTCGGGCAGC AGCCGACGGA CACTCCCTAC GAGTGGGACC TGGACAAAAA GGCTTGGTTC 
CCCAAGATTA CTGAAGATTT CATTGCTACA TATCAGGCCA ATTATGGCTT CTCTAACGAT 
GGCGCATCTA GTTCTACCGC AAATGTTGAA GATGTCCATG CTAGGACTGC AGAGGAACCT 
CCACAAGAAA AAGCCCCGGA ACCCACTGAT GCCAGAAAGA AGGGAGAAAA AAGAAAGGCT 
GAGTCAGGAT GGTTTCATGT TGAAGAAGAC AGAAATACAA ATGTATACGT GTCTGGTTTG 
CCTCCAGATA TTACAGTGGA TGAATTTATA CAACTTATGT CCAAGTTTGG CATTATTATG 
AGAGATCCTC AGACAGAAGA ATTTAAGGTC AAACTTTACA AAGATAATCA AGGAAATCTT 
AAAGGAGACG GTCTTTGCTG TTATTTGAAA AGAGAATCTG TGGAACTTGC ATTAAAACTT 
TTGGATGAAG ATGAAATTAG AGGCTACAAA TTACATGTTG AGGTGGCAAA GTTTCAACTG 
AAGGGAGAAT ATGATGCCTC AAAGAAGAAG AAGAAGTGCA AAGACTATAA GAAGAAGCTG 
TCTATGCAAC AAAAGCAGTT GGATTGGAGA CCTGAGAGGC GAGCCGGACC ATCCCGGATG 
CGCCATGAGC GAGTTGTCAT CATCAAGAAT ATGTTTCATC CTATGGATTT TGAGGATGAT 
CCGTTGGTGC TGAATGAGAT CAGAGAAGAC CTTCGAGTAG AGTGTTCGAA GTTTGGACAA 
ATTAGGAAAC TCCTTCTCTT TGATAGGCAC CCAGATGGTG TGGCCTCTGT GTCCTTTCGG 
GATCCAGAGG AAGCTGATTA TTGTATTCAG ACTCTCGATG GAAGATGGTT TGGTGGCCGT 
CAAATCACTG CCCAGGCATG GGATGGGACT ACAGATTATC AGGTGGAGGA AACCTCAAGA 
GAAAGGGAGG AAAGGCTGAG AGGATGGGAG GCTTTCCTCA ATGCTCCTGA GGCCAACAGA 
GGCCTTAGGC GTTCAGATTC TGTCTCTGCT TCCGAAAGGG CAGGGCCTTC TAGAGCAAGG 
CATTTTTCAG AGCACCCCAG CACATCTAAA ATGAATGCTC AAGAAACTGC AACTGGAATG 
GCGTTTGAAG AACCTATAGA TGAGAAGAAG TTTGAAAAGA CAGAAGATGG GGGAGAATTT 
GAAGAAGGTG CTTCTGAAAA CAATGCTAAG GAAAGTAGCC CCGAAAAAGA GGCTGAAGAA 
GGCTGCCCTG AAAAAGAATC TGAAGAGGGC TGCCCCAAAA GAGGGTTTGA AGGCAGCTGC 
TCCCAAAAAG AGTCTGAAGA AGGCAATCCC GTAAGAGGAT CTGAAGAGGA TAGTCCTAAA 
AAAGAGTCTA AAAAGAAGAC ACTCAAAAAT GATTGTGAAG AGAATGGCCT TGCAAAGGAA 
TCTGAAGATG ACCTCAACAA GGAGTCTGAA GAGGAGGTTG GCCCCACAAA AGAGTCCGAA 
GAAGATGACT CAGAGAAAGA GTCTGATGAA GACTGCTCTG AAAAACAGTC TGAAGATGGC 
TCCGAAAGAG AATTTGAAGA AAATGGTCTC GAGAAAGATT TGGACGAGGA AGGTTCTGAA 
AAGGAGCTTC ATGAAAATGT TCTTGACAAA GAGTTAGAAG AAAATGACTC TGAAAACTCC 
GAATTTGAAG ATGACGGCTC TGAAAAAGTG TTAGATGAGG AAGGCTCTGA GAGAGAGTTT 
GACGAAGATT CAGATGAAAA GGAAGAAGAG GAGGATACAT ATGAAAAAGT ATTTGATGAT 
GAGTCTGATG AGAAAGAGGA TGAAGAATAT GCAGATGAAA AGGGGCTTGA AGCTGCTGAT 
AAAAAGGCGG AAGAAGGTGA TGCAGATGAA AAGCTGTTTG AAGAGTCAGA TGACAAGGAA 
GATGAAGATG CAGATGGAAA GGAAGTTGAA GATGCTGACG AAAAGTTGTT CGAAGATGAT 
GATTCCAATG AGAAGTTGTT TGATGAGGAG GAAGATTCCA GTGAGAAGTT GTTTGACGAT 
TCTGATGAGA GGGGGACTTT GGGTGGTTTT GGGAGTGTTG AAGAAGGGCC CCTATCCACT 
GGCAGCAGCT TTATTCTCAG TAGCGATGAT GATGACGATG ATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HTATSF1 ( NM_001163280.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001156752.1 Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 1, mRNA.

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CloneID	OHu18558
Clone ID Related Accession (Same CDS sequence)	NM_014500.4 , XM_005262404.3 , XM_005262404.4 , NM_014500.5 , NM_001163280.1
Accession Version	NM_001163280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	HIV Tat-specific factor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB209303.1, BC009896.2 and BU633070.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209303.1, SRR1660805.87940.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGGCA CCAACTTGGA TGGGAACGAT GAGTTTGATG AGCAGTTGCG AATGCAAGAA 
TTGTACGGAG ACGGCAAGGA TGGTGACACC CAGACCGATG CCGGCGGAGA ACCCGATTCT 
CTCGGGCAGC AGCCGACGGA CACTCCCTAC GAGTGGGACC TGGACAAAAA GGCTTGGTTC 
CCCAAGATTA CTGAAGATTT CATTGCTACA TATCAGGCCA ATTATGGCTT CTCTAACGAT 
GGCGCATCTA GTTCTACCGC AAATGTTGAA GATGTCCATG CTAGGACTGC AGAGGAACCT 
CCACAAGAAA AAGCCCCGGA ACCCACTGAT GCCAGAAAGA AGGGAGAAAA AAGAAAGGCT 
GAGTCAGGAT GGTTTCATGT TGAAGAAGAC AGAAATACAA ATGTATACGT GTCTGGTTTG 
CCTCCAGATA TTACAGTGGA TGAATTTATA CAACTTATGT CCAAGTTTGG CATTATTATG 
AGAGATCCTC AGACAGAAGA ATTTAAGGTC AAACTTTACA AAGATAATCA AGGAAATCTT 
AAAGGAGACG GTCTTTGCTG TTATTTGAAA AGAGAATCTG TGGAACTTGC ATTAAAACTT 
TTGGATGAAG ATGAAATTAG AGGCTACAAA TTACATGTTG AGGTGGCAAA GTTTCAACTG 
AAGGGAGAAT ATGATGCCTC AAAGAAGAAG AAGAAGTGCA AAGACTATAA GAAGAAGCTG 
TCTATGCAAC AAAAGCAGTT GGATTGGAGA CCTGAGAGGC GAGCCGGACC ATCCCGGATG 
CGCCATGAGC GAGTTGTCAT CATCAAGAAT ATGTTTCATC CTATGGATTT TGAGGATGAT 
CCGTTGGTGC TGAATGAGAT CAGAGAAGAC CTTCGAGTAG AGTGTTCGAA GTTTGGACAA 
ATTAGGAAAC TCCTTCTCTT TGATAGGCAC CCAGATGGTG TGGCCTCTGT GTCCTTTCGG 
GATCCAGAGG AAGCTGATTA TTGTATTCAG ACTCTCGATG GAAGATGGTT TGGTGGCCGT 
CAAATCACTG CCCAGGCATG GGATGGGACT ACAGATTATC AGGTGGAGGA AACCTCAAGA 
GAAAGGGAGG AAAGGCTGAG AGGATGGGAG GCTTTCCTCA ATGCTCCTGA GGCCAACAGA 
GGCCTTAGGC GTTCAGATTC TGTCTCTGCT TCCGAAAGGG CAGGGCCTTC TAGAGCAAGG 
CATTTTTCAG AGCACCCCAG CACATCTAAA ATGAATGCTC AAGAAACTGC AACTGGAATG 
GCGTTTGAAG AACCTATAGA TGAGAAGAAG TTTGAAAAGA CAGAAGATGG GGGAGAATTT 
GAAGAAGGTG CTTCTGAAAA CAATGCTAAG GAAAGTAGCC CCGAAAAAGA GGCTGAAGAA 
GGCTGCCCTG AAAAAGAATC TGAAGAGGGC TGCCCCAAAA GAGGGTTTGA AGGCAGCTGC 
TCCCAAAAAG AGTCTGAAGA AGGCAATCCC GTAAGAGGAT CTGAAGAGGA TAGTCCTAAA 
AAAGAGTCTA AAAAGAAGAC ACTCAAAAAT GATTGTGAAG AGAATGGCCT TGCAAAGGAA 
TCTGAAGATG ACCTCAACAA GGAGTCTGAA GAGGAGGTTG GCCCCACAAA AGAGTCCGAA 
GAAGATGACT CAGAGAAAGA GTCTGATGAA GACTGCTCTG AAAAACAGTC TGAAGATGGC 
TCCGAAAGAG AATTTGAAGA AAATGGTCTC GAGAAAGATT TGGACGAGGA AGGTTCTGAA 
AAGGAGCTTC ATGAAAATGT TCTTGACAAA GAGTTAGAAG AAAATGACTC TGAAAACTCC 
GAATTTGAAG ATGACGGCTC TGAAAAAGTG TTAGATGAGG AAGGCTCTGA GAGAGAGTTT 
GACGAAGATT CAGATGAAAA GGAAGAAGAG GAGGATACAT ATGAAAAAGT ATTTGATGAT 
GAGTCTGATG AGAAAGAGGA TGAAGAATAT GCAGATGAAA AGGGGCTTGA AGCTGCTGAT 
AAAAAGGCGG AAGAAGGTGA TGCAGATGAA AAGCTGTTTG AAGAGTCAGA TGACAAGGAA 
GATGAAGATG CAGATGGAAA GGAAGTTGAA GATGCTGACG AAAAGTTGTT CGAAGATGAT 
GATTCCAATG AGAAGTTGTT TGATGAGGAG GAAGATTCCA GTGAGAAGTT GTTTGACGAT 
TCTGATGAGA GGGGGACTTT GGGTGGTTTT GGGAGTGTTG AAGAAGGGCC CCTATCCACT 
GGCAGCAGCT TTATTCTCAG TAGCGATGAT GATGACGATG ATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156752.1
CDS	423..2690
Translation	MSGTNLDGNDEFDEQLRMQELYGDGKDGDTQTDAGGEPDSLGQQPTDTPYEWDLDKKAWFPKITEDFIATYQANYGFSNDGASSSTANVEDVHARTAEEPPQEKAPEPTDARKKGEKRKAESGWFHVEEDRNTNVYVSGLPPDITVDEFIQLMSKFGIIMRDPQTEEFKVKLYKDNQGNLKGDGLCCYLKRESVELALKLLDEDEIRGYKLHVEVAKFQLKGEYDASKKKKKCKDYKKKLSMQQKQLDWRPERRAGPSRMRHERVVIIKNMFHPMDFEDDPLVLNEIREDLRVECSKFGQIRKLLLFDRHPDGVASVSFRDPEEADYCIQTLDGRWFGGRQITAQAWDGTTDYQVEETSREREERLRGWEAFLNAPEANRGLRRSDSVSASERAGPSRARHFSEHPSTSKMNAQETATGMAFEEPIDEKKFEKTEDGGEFEEGASENNAKESSPEKEAEEGCPEKESEEGCPKRGFEGSCSQKESEEGNPVRGSEEDSPKKESKKKTLKNDCEENGLAKESEDDLNKESEEEVGPTKESEEDDSEKESDEDCSEKQSEDGSEREFEENGLEKDLDEEGSEKELHENVLDKELEENDSENSEFEDDGSEKVLDEEGSEREFDEDSDEKEEEEDTYEKVFDDESDEKEDEEYADEKGLEAADKKAEEGDADEKLFEESDDKEDEDADGKEVEDADEKLFEDDDSNEKLFDEEEDSSEKLFDDSDERGTLGGFGSVEEGPLSTGSSFILSSDDDDDDI

Target ORF information:

RefSeq Version	NM_001163280.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163280.1

ORF Insert Sequence:

ATGAGCGGCA CCAACTTGGA TGGGAACGAT GAGTTTGATG AGCAGTTGCG AATGCAAGAA 
TTGTACGGAG ACGGCAAGGA TGGTGACACC CAGACCGATG CCGGCGGAGA ACCCGATTCT 
CTCGGGCAGC AGCCGACGGA CACTCCCTAC GAGTGGGACC TGGACAAAAA GGCTTGGTTC 
CCCAAGATTA CTGAAGATTT CATTGCTACA TATCAGGCCA ATTATGGCTT CTCTAACGAT 
GGCGCATCTA GTTCTACCGC AAATGTTGAA GATGTCCATG CTAGGACTGC AGAGGAACCT 
CCACAAGAAA AAGCCCCGGA ACCCACTGAT GCCAGAAAGA AGGGAGAAAA AAGAAAGGCT 
GAGTCAGGAT GGTTTCATGT TGAAGAAGAC AGAAATACAA ATGTATACGT GTCTGGTTTG 
CCTCCAGATA TTACAGTGGA TGAATTTATA CAACTTATGT CCAAGTTTGG CATTATTATG 
AGAGATCCTC AGACAGAAGA ATTTAAGGTC AAACTTTACA AAGATAATCA AGGAAATCTT 
AAAGGAGACG GTCTTTGCTG TTATTTGAAA AGAGAATCTG TGGAACTTGC ATTAAAACTT 
TTGGATGAAG ATGAAATTAG AGGCTACAAA TTACATGTTG AGGTGGCAAA GTTTCAACTG 
AAGGGAGAAT ATGATGCCTC AAAGAAGAAG AAGAAGTGCA AAGACTATAA GAAGAAGCTG 
TCTATGCAAC AAAAGCAGTT GGATTGGAGA CCTGAGAGGC GAGCCGGACC ATCCCGGATG 
CGCCATGAGC GAGTTGTCAT CATCAAGAAT ATGTTTCATC CTATGGATTT TGAGGATGAT 
CCGTTGGTGC TGAATGAGAT CAGAGAAGAC CTTCGAGTAG AGTGTTCGAA GTTTGGACAA 
ATTAGGAAAC TCCTTCTCTT TGATAGGCAC CCAGATGGTG TGGCCTCTGT GTCCTTTCGG 
GATCCAGAGG AAGCTGATTA TTGTATTCAG ACTCTCGATG GAAGATGGTT TGGTGGCCGT 
CAAATCACTG CCCAGGCATG GGATGGGACT ACAGATTATC AGGTGGAGGA AACCTCAAGA 
GAAAGGGAGG AAAGGCTGAG AGGATGGGAG GCTTTCCTCA ATGCTCCTGA GGCCAACAGA 
GGCCTTAGGC GTTCAGATTC TGTCTCTGCT TCCGAAAGGG CAGGGCCTTC TAGAGCAAGG 
CATTTTTCAG AGCACCCCAG CACATCTAAA ATGAATGCTC AAGAAACTGC AACTGGAATG 
GCGTTTGAAG AACCTATAGA TGAGAAGAAG TTTGAAAAGA CAGAAGATGG GGGAGAATTT 
GAAGAAGGTG CTTCTGAAAA CAATGCTAAG GAAAGTAGCC CCGAAAAAGA GGCTGAAGAA 
GGCTGCCCTG AAAAAGAATC TGAAGAGGGC TGCCCCAAAA GAGGGTTTGA AGGCAGCTGC 
TCCCAAAAAG AGTCTGAAGA AGGCAATCCC GTAAGAGGAT CTGAAGAGGA TAGTCCTAAA 
AAAGAGTCTA AAAAGAAGAC ACTCAAAAAT GATTGTGAAG AGAATGGCCT TGCAAAGGAA 
TCTGAAGATG ACCTCAACAA GGAGTCTGAA GAGGAGGTTG GCCCCACAAA AGAGTCCGAA 
GAAGATGACT CAGAGAAAGA GTCTGATGAA GACTGCTCTG AAAAACAGTC TGAAGATGGC 
TCCGAAAGAG AATTTGAAGA AAATGGTCTC GAGAAAGATT TGGACGAGGA AGGTTCTGAA 
AAGGAGCTTC ATGAAAATGT TCTTGACAAA GAGTTAGAAG AAAATGACTC TGAAAACTCC 
GAATTTGAAG ATGACGGCTC TGAAAAAGTG TTAGATGAGG AAGGCTCTGA GAGAGAGTTT 
GACGAAGATT CAGATGAAAA GGAAGAAGAG GAGGATACAT ATGAAAAAGT ATTTGATGAT 
GAGTCTGATG AGAAAGAGGA TGAAGAATAT GCAGATGAAA AGGGGCTTGA AGCTGCTGAT 
AAAAAGGCGG AAGAAGGTGA TGCAGATGAA AAGCTGTTTG AAGAGTCAGA TGACAAGGAA 
GATGAAGATG CAGATGGAAA GGAAGTTGAA GATGCTGACG AAAAGTTGTT CGAAGATGAT 
GATTCCAATG AGAAGTTGTT TGATGAGGAG GAAGATTCCA GTGAGAAGTT GTTTGACGAT 
TCTGATGAGA GGGGGACTTT GGGTGGTTTT GGGAGTGTTG AAGAAGGGCC CCTATCCACT 
GGCAGCAGCT TTATTCTCAG TAGCGATGAT GATGACGATG ATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HTATSF1 ( XM_005262404.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005262461.1 Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu18558
Clone ID Related Accession (Same CDS sequence)	NM_014500.4 , XM_005262404.3 , XM_005262404.4 , NM_014500.5 , NM_001163280.1
Accession Version	XM_005262404.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	HIV Tat-specific factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005262404.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCA CCAACTTGGA TGGGAACGAT GAGTTTGATG AGCAGTTGCG AATGCAAGAA 
TTGTACGGAG ACGGCAAGGA TGGTGACACC CAGACCGATG CCGGCGGAGA ACCCGATTCT 
CTCGGGCAGC AGCCGACGGA CACTCCCTAC GAGTGGGACC TGGACAAAAA GGCTTGGTTC 
CCCAAGATTA CTGAAGATTT CATTGCTACA TATCAGGCCA ATTATGGCTT CTCTAACGAT 
GGCGCATCTA GTTCTACCGC AAATGTTGAA GATGTCCATG CTAGGACTGC AGAGGAACCT 
CCACAAGAAA AAGCCCCGGA ACCCACTGAT GCCAGAAAGA AGGGAGAAAA AAGAAAGGCT 
GAGTCAGGAT GGTTTCATGT TGAAGAAGAC AGAAATACAA ATGTATACGT GTCTGGTTTG 
CCTCCAGATA TTACAGTGGA TGAATTTATA CAACTTATGT CCAAGTTTGG CATTATTATG 
AGAGATCCTC AGACAGAAGA ATTTAAGGTC AAACTTTACA AAGATAATCA AGGAAATCTT 
AAAGGAGACG GTCTTTGCTG TTATTTGAAA AGAGAATCTG TGGAACTTGC ATTAAAACTT 
TTGGATGAAG ATGAAATTAG AGGCTACAAA TTACATGTTG AGGTGGCAAA GTTTCAACTG 
AAGGGAGAAT ATGATGCCTC AAAGAAGAAG AAGAAGTGCA AAGACTATAA GAAGAAGCTG 
TCTATGCAAC AAAAGCAGTT GGATTGGAGA CCTGAGAGGC GAGCCGGACC ATCCCGGATG 
CGCCATGAGC GAGTTGTCAT CATCAAGAAT ATGTTTCATC CTATGGATTT TGAGGATGAT 
CCGTTGGTGC TGAATGAGAT CAGAGAAGAC CTTCGAGTAG AGTGTTCGAA GTTTGGACAA 
ATTAGGAAAC TCCTTCTCTT TGATAGGCAC CCAGATGGTG TGGCCTCTGT GTCCTTTCGG 
GATCCAGAGG AAGCTGATTA TTGTATTCAG ACTCTCGATG GAAGATGGTT TGGTGGCCGT 
CAAATCACTG CCCAGGCATG GGATGGGACT ACAGATTATC AGGTGGAGGA AACCTCAAGA 
GAAAGGGAGG AAAGGCTGAG AGGATGGGAG GCTTTCCTCA ATGCTCCTGA GGCCAACAGA 
GGCCTTAGGC GTTCAGATTC TGTCTCTGCT TCCGAAAGGG CAGGGCCTTC TAGAGCAAGG 
CATTTTTCAG AGCACCCCAG CACATCTAAA ATGAATGCTC AAGAAACTGC AACTGGAATG 
GCGTTTGAAG AACCTATAGA TGAGAAGAAG TTTGAAAAGA CAGAAGATGG GGGAGAATTT 
GAAGAAGGTG CTTCTGAAAA CAATGCTAAG GAAAGTAGCC CCGAAAAAGA GGCTGAAGAA 
GGCTGCCCTG AAAAAGAATC TGAAGAGGGC TGCCCCAAAA GAGGGTTTGA AGGCAGCTGC 
TCCCAAAAAG AGTCTGAAGA AGGCAATCCC GTAAGAGGAT CTGAAGAGGA TAGTCCTAAA 
AAAGAGTCTA AAAAGAAGAC ACTCAAAAAT GATTGTGAAG AGAATGGCCT TGCAAAGGAA 
TCTGAAGATG ACCTCAACAA GGAGTCTGAA GAGGAGGTTG GCCCCACAAA AGAGTCCGAA 
GAAGATGACT CAGAGAAAGA GTCTGATGAA GACTGCTCTG AAAAACAGTC TGAAGATGGC 
TCCGAAAGAG AATTTGAAGA AAATGGTCTC GAGAAAGATT TGGACGAGGA AGGTTCTGAA 
AAGGAGCTTC ATGAAAATGT TCTTGACAAA GAGTTAGAAG AAAATGACTC TGAAAACTCC 
GAATTTGAAG ATGACGGCTC TGAAAAAGTG TTAGATGAGG AAGGCTCTGA GAGAGAGTTT 
GACGAAGATT CAGATGAAAA GGAAGAAGAG GAGGATACAT ATGAAAAAGT ATTTGATGAT 
GAGTCTGATG AGAAAGAGGA TGAAGAATAT GCAGATGAAA AGGGGCTTGA AGCTGCTGAT 
AAAAAGGCGG AAGAAGGTGA TGCAGATGAA AAGCTGTTTG AAGAGTCAGA TGACAAGGAA 
GATGAAGATG CAGATGGAAA GGAAGTTGAA GATGCTGACG AAAAGTTGTT CGAAGATGAT 
GATTCCAATG AGAAGTTGTT TGATGAGGAG GAAGATTCCA GTGAGAAGTT GTTTGACGAT 
TCTGATGAGA GGGGGACTTT GGGTGGTTTT GGGAGTGTTG AAGAAGGGCC CCTATCCACT 
GGCAGCAGCT TTATTCTCAG TAGCGATGAT GATGACGATG ATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005262461.1
CDS	518..2785
Translation	MSGTNLDGNDEFDEQLRMQELYGDGKDGDTQTDAGGEPDSLGQQPTDTPYEWDLDKKAWFPKITEDFIATYQANYGFSNDGASSSTANVEDVHARTAEEPPQEKAPEPTDARKKGEKRKAESGWFHVEEDRNTNVYVSGLPPDITVDEFIQLMSKFGIIMRDPQTEEFKVKLYKDNQGNLKGDGLCCYLKRESVELALKLLDEDEIRGYKLHVEVAKFQLKGEYDASKKKKKCKDYKKKLSMQQKQLDWRPERRAGPSRMRHERVVIIKNMFHPMDFEDDPLVLNEIREDLRVECSKFGQIRKLLLFDRHPDGVASVSFRDPEEADYCIQTLDGRWFGGRQITAQAWDGTTDYQVEETSREREERLRGWEAFLNAPEANRGLRRSDSVSASERAGPSRARHFSEHPSTSKMNAQETATGMAFEEPIDEKKFEKTEDGGEFEEGASENNAKESSPEKEAEEGCPEKESEEGCPKRGFEGSCSQKESEEGNPVRGSEEDSPKKESKKKTLKNDCEENGLAKESEDDLNKESEEEVGPTKESEEDDSEKESDEDCSEKQSEDGSEREFEENGLEKDLDEEGSEKELHENVLDKELEENDSENSEFEDDGSEKVLDEEGSEREFDEDSDEKEEEEDTYEKVFDDESDEKEDEEYADEKGLEAADKKAEEGDADEKLFEESDDKEDEDADGKEVEDADEKLFEDDDSNEKLFDEEEDSSEKLFDDSDERGTLGGFGSVEEGPLSTGSSFILSSDDDDDDI

Target ORF information:

RefSeq Version	XM_005262404.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262404.3

ORF Insert Sequence:

ATGAGCGGCA CCAACTTGGA TGGGAACGAT GAGTTTGATG AGCAGTTGCG AATGCAAGAA 
TTGTACGGAG ACGGCAAGGA TGGTGACACC CAGACCGATG CCGGCGGAGA ACCCGATTCT 
CTCGGGCAGC AGCCGACGGA CACTCCCTAC GAGTGGGACC TGGACAAAAA GGCTTGGTTC 
CCCAAGATTA CTGAAGATTT CATTGCTACA TATCAGGCCA ATTATGGCTT CTCTAACGAT 
GGCGCATCTA GTTCTACCGC AAATGTTGAA GATGTCCATG CTAGGACTGC AGAGGAACCT 
CCACAAGAAA AAGCCCCGGA ACCCACTGAT GCCAGAAAGA AGGGAGAAAA AAGAAAGGCT 
GAGTCAGGAT GGTTTCATGT TGAAGAAGAC AGAAATACAA ATGTATACGT GTCTGGTTTG 
CCTCCAGATA TTACAGTGGA TGAATTTATA CAACTTATGT CCAAGTTTGG CATTATTATG 
AGAGATCCTC AGACAGAAGA ATTTAAGGTC AAACTTTACA AAGATAATCA AGGAAATCTT 
AAAGGAGACG GTCTTTGCTG TTATTTGAAA AGAGAATCTG TGGAACTTGC ATTAAAACTT 
TTGGATGAAG ATGAAATTAG AGGCTACAAA TTACATGTTG AGGTGGCAAA GTTTCAACTG 
AAGGGAGAAT ATGATGCCTC AAAGAAGAAG AAGAAGTGCA AAGACTATAA GAAGAAGCTG 
TCTATGCAAC AAAAGCAGTT GGATTGGAGA CCTGAGAGGC GAGCCGGACC ATCCCGGATG 
CGCCATGAGC GAGTTGTCAT CATCAAGAAT ATGTTTCATC CTATGGATTT TGAGGATGAT 
CCGTTGGTGC TGAATGAGAT CAGAGAAGAC CTTCGAGTAG AGTGTTCGAA GTTTGGACAA 
ATTAGGAAAC TCCTTCTCTT TGATAGGCAC CCAGATGGTG TGGCCTCTGT GTCCTTTCGG 
GATCCAGAGG AAGCTGATTA TTGTATTCAG ACTCTCGATG GAAGATGGTT TGGTGGCCGT 
CAAATCACTG CCCAGGCATG GGATGGGACT ACAGATTATC AGGTGGAGGA AACCTCAAGA 
GAAAGGGAGG AAAGGCTGAG AGGATGGGAG GCTTTCCTCA ATGCTCCTGA GGCCAACAGA 
GGCCTTAGGC GTTCAGATTC TGTCTCTGCT TCCGAAAGGG CAGGGCCTTC TAGAGCAAGG 
CATTTTTCAG AGCACCCCAG CACATCTAAA ATGAATGCTC AAGAAACTGC AACTGGAATG 
GCGTTTGAAG AACCTATAGA TGAGAAGAAG TTTGAAAAGA CAGAAGATGG GGGAGAATTT 
GAAGAAGGTG CTTCTGAAAA CAATGCTAAG GAAAGTAGCC CCGAAAAAGA GGCTGAAGAA 
GGCTGCCCTG AAAAAGAATC TGAAGAGGGC TGCCCCAAAA GAGGGTTTGA AGGCAGCTGC 
TCCCAAAAAG AGTCTGAAGA AGGCAATCCC GTAAGAGGAT CTGAAGAGGA TAGTCCTAAA 
AAAGAGTCTA AAAAGAAGAC ACTCAAAAAT GATTGTGAAG AGAATGGCCT TGCAAAGGAA 
TCTGAAGATG ACCTCAACAA GGAGTCTGAA GAGGAGGTTG GCCCCACAAA AGAGTCCGAA 
GAAGATGACT CAGAGAAAGA GTCTGATGAA GACTGCTCTG AAAAACAGTC TGAAGATGGC 
TCCGAAAGAG AATTTGAAGA AAATGGTCTC GAGAAAGATT TGGACGAGGA AGGTTCTGAA 
AAGGAGCTTC ATGAAAATGT TCTTGACAAA GAGTTAGAAG AAAATGACTC TGAAAACTCC 
GAATTTGAAG ATGACGGCTC TGAAAAAGTG TTAGATGAGG AAGGCTCTGA GAGAGAGTTT 
GACGAAGATT CAGATGAAAA GGAAGAAGAG GAGGATACAT ATGAAAAAGT ATTTGATGAT 
GAGTCTGATG AGAAAGAGGA TGAAGAATAT GCAGATGAAA AGGGGCTTGA AGCTGCTGAT 
AAAAAGGCGG AAGAAGGTGA TGCAGATGAA AAGCTGTTTG AAGAGTCAGA TGACAAGGAA 
GATGAAGATG CAGATGGAAA GGAAGTTGAA GATGCTGACG AAAAGTTGTT CGAAGATGAT 
GATTCCAATG AGAAGTTGTT TGATGAGGAG GAAGATTCCA GTGAGAAGTT GTTTGACGAT 
TCTGATGAGA GGGGGACTTT GGGTGGTTTT GGGAGTGTTG AAGAAGGGCC CCTATCCACT 
GGCAGCAGCT TTATTCTCAG TAGCGATGAT GATGACGATG ATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HTATSF1 ( XM_005262404.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005262461.1 Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu18558
Clone ID Related Accession (Same CDS sequence)	NM_014500.4 , XM_005262404.3 , XM_005262404.4 , NM_014500.5 , NM_001163280.1
Accession Version	XM_005262404.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	HIV Tat-specific factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005262404.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCA CCAACTTGGA TGGGAACGAT GAGTTTGATG AGCAGTTGCG AATGCAAGAA 
TTGTACGGAG ACGGCAAGGA TGGTGACACC CAGACCGATG CCGGCGGAGA ACCCGATTCT 
CTCGGGCAGC AGCCGACGGA CACTCCCTAC GAGTGGGACC TGGACAAAAA GGCTTGGTTC 
CCCAAGATTA CTGAAGATTT CATTGCTACA TATCAGGCCA ATTATGGCTT CTCTAACGAT 
GGCGCATCTA GTTCTACCGC AAATGTTGAA GATGTCCATG CTAGGACTGC AGAGGAACCT 
CCACAAGAAA AAGCCCCGGA ACCCACTGAT GCCAGAAAGA AGGGAGAAAA AAGAAAGGCT 
GAGTCAGGAT GGTTTCATGT TGAAGAAGAC AGAAATACAA ATGTATACGT GTCTGGTTTG 
CCTCCAGATA TTACAGTGGA TGAATTTATA CAACTTATGT CCAAGTTTGG CATTATTATG 
AGAGATCCTC AGACAGAAGA ATTTAAGGTC AAACTTTACA AAGATAATCA AGGAAATCTT 
AAAGGAGACG GTCTTTGCTG TTATTTGAAA AGAGAATCTG TGGAACTTGC ATTAAAACTT 
TTGGATGAAG ATGAAATTAG AGGCTACAAA TTACATGTTG AGGTGGCAAA GTTTCAACTG 
AAGGGAGAAT ATGATGCCTC AAAGAAGAAG AAGAAGTGCA AAGACTATAA GAAGAAGCTG 
TCTATGCAAC AAAAGCAGTT GGATTGGAGA CCTGAGAGGC GAGCCGGACC ATCCCGGATG 
CGCCATGAGC GAGTTGTCAT CATCAAGAAT ATGTTTCATC CTATGGATTT TGAGGATGAT 
CCGTTGGTGC TGAATGAGAT CAGAGAAGAC CTTCGAGTAG AGTGTTCGAA GTTTGGACAA 
ATTAGGAAAC TCCTTCTCTT TGATAGGCAC CCAGATGGTG TGGCCTCTGT GTCCTTTCGG 
GATCCAGAGG AAGCTGATTA TTGTATTCAG ACTCTCGATG GAAGATGGTT TGGTGGCCGT 
CAAATCACTG CCCAGGCATG GGATGGGACT ACAGATTATC AGGTGGAGGA AACCTCAAGA 
GAAAGGGAGG AAAGGCTGAG AGGATGGGAG GCTTTCCTCA ATGCTCCTGA GGCCAACAGA 
GGCCTTAGGC GTTCAGATTC TGTCTCTGCT TCCGAAAGGG CAGGGCCTTC TAGAGCAAGG 
CATTTTTCAG AGCACCCCAG CACATCTAAA ATGAATGCTC AAGAAACTGC AACTGGAATG 
GCGTTTGAAG AACCTATAGA TGAGAAGAAG TTTGAAAAGA CAGAAGATGG GGGAGAATTT 
GAAGAAGGTG CTTCTGAAAA CAATGCTAAG GAAAGTAGCC CCGAAAAAGA GGCTGAAGAA 
GGCTGCCCTG AAAAAGAATC TGAAGAGGGC TGCCCCAAAA GAGGGTTTGA AGGCAGCTGC 
TCCCAAAAAG AGTCTGAAGA AGGCAATCCC GTAAGAGGAT CTGAAGAGGA TAGTCCTAAA 
AAAGAGTCTA AAAAGAAGAC ACTCAAAAAT GATTGTGAAG AGAATGGCCT TGCAAAGGAA 
TCTGAAGATG ACCTCAACAA GGAGTCTGAA GAGGAGGTTG GCCCCACAAA AGAGTCCGAA 
GAAGATGACT CAGAGAAAGA GTCTGATGAA GACTGCTCTG AAAAACAGTC TGAAGATGGC 
TCCGAAAGAG AATTTGAAGA AAATGGTCTC GAGAAAGATT TGGACGAGGA AGGTTCTGAA 
AAGGAGCTTC ATGAAAATGT TCTTGACAAA GAGTTAGAAG AAAATGACTC TGAAAACTCC 
GAATTTGAAG ATGACGGCTC TGAAAAAGTG TTAGATGAGG AAGGCTCTGA GAGAGAGTTT 
GACGAAGATT CAGATGAAAA GGAAGAAGAG GAGGATACAT ATGAAAAAGT ATTTGATGAT 
GAGTCTGATG AGAAAGAGGA TGAAGAATAT GCAGATGAAA AGGGGCTTGA AGCTGCTGAT 
AAAAAGGCGG AAGAAGGTGA TGCAGATGAA AAGCTGTTTG AAGAGTCAGA TGACAAGGAA 
GATGAAGATG CAGATGGAAA GGAAGTTGAA GATGCTGACG AAAAGTTGTT CGAAGATGAT 
GATTCCAATG AGAAGTTGTT TGATGAGGAG GAAGATTCCA GTGAGAAGTT GTTTGACGAT 
TCTGATGAGA GGGGGACTTT GGGTGGTTTT GGGAGTGTTG AAGAAGGGCC CCTATCCACT 
GGCAGCAGCT TTATTCTCAG TAGCGATGAT GATGACGATG ATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005262461.1
CDS	223..2490
Translation	MSGTNLDGNDEFDEQLRMQELYGDGKDGDTQTDAGGEPDSLGQQPTDTPYEWDLDKKAWFPKITEDFIATYQANYGFSNDGASSSTANVEDVHARTAEEPPQEKAPEPTDARKKGEKRKAESGWFHVEEDRNTNVYVSGLPPDITVDEFIQLMSKFGIIMRDPQTEEFKVKLYKDNQGNLKGDGLCCYLKRESVELALKLLDEDEIRGYKLHVEVAKFQLKGEYDASKKKKKCKDYKKKLSMQQKQLDWRPERRAGPSRMRHERVVIIKNMFHPMDFEDDPLVLNEIREDLRVECSKFGQIRKLLLFDRHPDGVASVSFRDPEEADYCIQTLDGRWFGGRQITAQAWDGTTDYQVEETSREREERLRGWEAFLNAPEANRGLRRSDSVSASERAGPSRARHFSEHPSTSKMNAQETATGMAFEEPIDEKKFEKTEDGGEFEEGASENNAKESSPEKEAEEGCPEKESEEGCPKRGFEGSCSQKESEEGNPVRGSEEDSPKKESKKKTLKNDCEENGLAKESEDDLNKESEEEVGPTKESEEDDSEKESDEDCSEKQSEDGSEREFEENGLEKDLDEEGSEKELHENVLDKELEENDSENSEFEDDGSEKVLDEEGSEREFDEDSDEKEEEEDTYEKVFDDESDEKEDEEYADEKGLEAADKKAEEGDADEKLFEESDDKEDEDADGKEVEDADEKLFEDDDSNEKLFDEEEDSSEKLFDDSDERGTLGGFGSVEEGPLSTGSSFILSSDDDDDDI

Target ORF information:

RefSeq Version	XM_005262404.4
Organism	Homo sapiens(human)
Definition	Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262404.4

ORF Insert Sequence:

ATGAGCGGCA CCAACTTGGA TGGGAACGAT GAGTTTGATG AGCAGTTGCG AATGCAAGAA 
TTGTACGGAG ACGGCAAGGA TGGTGACACC CAGACCGATG CCGGCGGAGA ACCCGATTCT 
CTCGGGCAGC AGCCGACGGA CACTCCCTAC GAGTGGGACC TGGACAAAAA GGCTTGGTTC 
CCCAAGATTA CTGAAGATTT CATTGCTACA TATCAGGCCA ATTATGGCTT CTCTAACGAT 
GGCGCATCTA GTTCTACCGC AAATGTTGAA GATGTCCATG CTAGGACTGC AGAGGAACCT 
CCACAAGAAA AAGCCCCGGA ACCCACTGAT GCCAGAAAGA AGGGAGAAAA AAGAAAGGCT 
GAGTCAGGAT GGTTTCATGT TGAAGAAGAC AGAAATACAA ATGTATACGT GTCTGGTTTG 
CCTCCAGATA TTACAGTGGA TGAATTTATA CAACTTATGT CCAAGTTTGG CATTATTATG 
AGAGATCCTC AGACAGAAGA ATTTAAGGTC AAACTTTACA AAGATAATCA AGGAAATCTT 
AAAGGAGACG GTCTTTGCTG TTATTTGAAA AGAGAATCTG TGGAACTTGC ATTAAAACTT 
TTGGATGAAG ATGAAATTAG AGGCTACAAA TTACATGTTG AGGTGGCAAA GTTTCAACTG 
AAGGGAGAAT ATGATGCCTC AAAGAAGAAG AAGAAGTGCA AAGACTATAA GAAGAAGCTG 
TCTATGCAAC AAAAGCAGTT GGATTGGAGA CCTGAGAGGC GAGCCGGACC ATCCCGGATG 
CGCCATGAGC GAGTTGTCAT CATCAAGAAT ATGTTTCATC CTATGGATTT TGAGGATGAT 
CCGTTGGTGC TGAATGAGAT CAGAGAAGAC CTTCGAGTAG AGTGTTCGAA GTTTGGACAA 
ATTAGGAAAC TCCTTCTCTT TGATAGGCAC CCAGATGGTG TGGCCTCTGT GTCCTTTCGG 
GATCCAGAGG AAGCTGATTA TTGTATTCAG ACTCTCGATG GAAGATGGTT TGGTGGCCGT 
CAAATCACTG CCCAGGCATG GGATGGGACT ACAGATTATC AGGTGGAGGA AACCTCAAGA 
GAAAGGGAGG AAAGGCTGAG AGGATGGGAG GCTTTCCTCA ATGCTCCTGA GGCCAACAGA 
GGCCTTAGGC GTTCAGATTC TGTCTCTGCT TCCGAAAGGG CAGGGCCTTC TAGAGCAAGG 
CATTTTTCAG AGCACCCCAG CACATCTAAA ATGAATGCTC AAGAAACTGC AACTGGAATG 
GCGTTTGAAG AACCTATAGA TGAGAAGAAG TTTGAAAAGA CAGAAGATGG GGGAGAATTT 
GAAGAAGGTG CTTCTGAAAA CAATGCTAAG GAAAGTAGCC CCGAAAAAGA GGCTGAAGAA 
GGCTGCCCTG AAAAAGAATC TGAAGAGGGC TGCCCCAAAA GAGGGTTTGA AGGCAGCTGC 
TCCCAAAAAG AGTCTGAAGA AGGCAATCCC GTAAGAGGAT CTGAAGAGGA TAGTCCTAAA 
AAAGAGTCTA AAAAGAAGAC ACTCAAAAAT GATTGTGAAG AGAATGGCCT TGCAAAGGAA 
TCTGAAGATG ACCTCAACAA GGAGTCTGAA GAGGAGGTTG GCCCCACAAA AGAGTCCGAA 
GAAGATGACT CAGAGAAAGA GTCTGATGAA GACTGCTCTG AAAAACAGTC TGAAGATGGC 
TCCGAAAGAG AATTTGAAGA AAATGGTCTC GAGAAAGATT TGGACGAGGA AGGTTCTGAA 
AAGGAGCTTC ATGAAAATGT TCTTGACAAA GAGTTAGAAG AAAATGACTC TGAAAACTCC 
GAATTTGAAG ATGACGGCTC TGAAAAAGTG TTAGATGAGG AAGGCTCTGA GAGAGAGTTT 
GACGAAGATT CAGATGAAAA GGAAGAAGAG GAGGATACAT ATGAAAAAGT ATTTGATGAT 
GAGTCTGATG AGAAAGAGGA TGAAGAATAT GCAGATGAAA AGGGGCTTGA AGCTGCTGAT 
AAAAAGGCGG AAGAAGGTGA TGCAGATGAA AAGCTGTTTG AAGAGTCAGA TGACAAGGAA 
GATGAAGATG CAGATGGAAA GGAAGTTGAA GATGCTGACG AAAAGTTGTT CGAAGATGAT 
GATTCCAATG AGAAGTTGTT TGATGAGGAG GAAGATTCCA GTGAGAAGTT GTTTGACGAT 
TCTGATGAGA GGGGGACTTT GGGTGGTTTT GGGAGTGTTG AAGAAGGGCC CCTATCCACT 
GGCAGCAGCT TTATTCTCAG TAGCGATGAT GATGACGATG ATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Impact of sustained RNAi-mediated suppression of cellular cofactor Tat-SF1 on HIV-1 replication in CD4+ T cells.
Virology journal9272(2012 Nov)
Green VA,Arbuthnot P,Weinberg MS

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Identification of Tat-SF1 cellular targets by exon array analysis reveals dual roles in transcription and splicing.
RNA (New York, N.Y.)17(4)665-74(2011 Apr)
Miller HB,Robinson TJ,Gord?n R,Hartemink AJ,Garcia-Blanco MA

DSIF, the Paf1 complex, and Tat-SF1 have nonredundant, cooperative roles in RNA polymerase II elongation.
Genes & development23(23)2765-77(2009 Dec)
Chen Y,Yamaguchi Y,Tsugeno Y,Yamamoto J,Yamada T,Nakamura M,Hisatake K,Handa H

Tat-SF1 is not required for Tat transactivation but does regulate the relative levels of unspliced and spliced HIV-1 RNAs.
PloS one4(5)e5710(2009 May)
Miller HB,Saunders KO,Tomaras GD,Garcia-Blanco MA

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HTATSF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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