APEX2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	APEX2
Entrez Gene ID	27301
Full Name	apurinic/apyrimidinic endodeoxyribonuclease 2
Synonyms	APE2,APEXL2,XTH2,ZGRF2
General protein information	Preferred Names apurinic/apyrimidinic endodeoxyribonuclease 2 Names DNA-(apurinic or apyrimidinic site) lyase 2 AP endonuclease 2 AP endonuclease XTH2 APEX nuclease (apurinic/apyrimidinic endonuclease) 2 apurinic/apyrimidinic endonuclease-like 2 zinc finger, GRF-type containing 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.21
Summary	Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012].
Disorder MIM:	300773

mRNA and Protein(s)

mRNA	Protein	Name
NM_001271748.1	NP_001258677.1	DNA-(apurinic or apyrimidinic site) lyase 2 isoform 2
NM_001271748.2	NP_001258677.1	DNA-(apurinic or apyrimidinic site) lyase 2 isoform 2
NM_014481.3	NP_055296.2	DNA-(apurinic or apyrimidinic site) lyase 2 isoform 1
NM_014481.4	NP_055296.2	DNA-(apurinic or apyrimidinic site) lyase 2 isoform 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	apex2	NP_956440.1
Oryza sativa (rice)	Os09g0536000	NP_001063783.1
Pan troglodytes (chimpanzee)	APEX2	XP_003317535.1
	APEX2	XP_001092143.1
Canis lupus familiaris (dog)	APEX2	XP_005641455.1
Neurospora crassa	NCU01961	XP_964325.2
Homo sapiens (human)	APEX2	NP_055296.2
Bos taurus (cattle)	APEX2	NP_001015577.1
Magnaporthe oryzae (rice blast fungus)	MGG_02980	XP_003720720.1
Mus musculus (house mouse)	Apex2	NP_084219.1
Schizosaccharomyces pombe (fission yeast)	apn2	NP_595522.1
Rattus norvegicus (Norway rat)	Apex2	NP_001073361.1
Danio rerio (zebrafish)	LOC101885601	XP_005167134.1
Arabidopsis thaliana (thale cress)	AT4G36050	NP_974691.2
Xenopus tropicalis (tropical clawed frog)	apex2	NP_001006804.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of PCNA-dependent stimulation of 3'-phosphodiesterase and 3'-5' exonuclease activities of human Ape2 in repair of oxidative DNA damage.
Burkovics P, Hajd? I, Szukacsov V, Unk I, Haracska L
Nucleic acids research37(13)4247-55(2009 Jul)

Human Ape2 protein has a 3'-5' exonuclease activity that acts preferentially on mismatched base pairs.
Burkovics P, Szukacsov V, Unk I, Haracska L
Nucleic acids research34(9)2508-15(2006)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Variation within DNA repair pathway genes and risk of multiple sclerosis.

Ape2 may be involved in repair of oxidative DNA damage and PCNA-dependent repair synthesis.
Title: Role of PCNA-dependent stimulation of 3'-phosphodiesterase and 3'-5' exonuclease activities of human Ape2 in repair of oxidative DNA damage.

Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia.
Title: Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia.

Ape2 exhibits strong 3'-5' exonuclease and 3'-phosphodiesterase activities and has only a very weak AP-endonuclease activity.
Title: Human Ape2 protein has a 3'-5' exonuclease activity that acts preferentially on mismatched base pairs.

Determinants in nuclease specificity of Ape1 and Ape2, human homologues of Escherichia coli exonuclease III
Title: Determinants in nuclease specificity of Ape1 and Ape2, human homologues of Escherichia coli exonuclease III.

The following APEX2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APEX2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20076	NM_014481.4 Latest version!	Homo sapiens apurinic/apyrimidinic endodeoxyribonuclease 2 (APEX2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu09706	NM_001271748.2 Latest version!	Homo sapiens apurinic/apyrimidinic endodeoxyribonuclease 2 (APEX2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
View Old Accession Versions >>
OHu09706	NM_001271748.1	Homo sapiens apurinic/apyrimidinic endodeoxyribonuclease 2 (APEX2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu20076	NM_014481.3	Homo sapiens apurinic/apyrimidinic endodeoxyribonuclease 2 (APEX2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APEX2 ( NM_014481.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055296.2 Homo sapiens apurinic/apyrimidinic endodeoxyribonuclease 2 (APEX2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20076
Clone ID Related Accession (Same CDS sequence)	NM_014481.3 , NM_014481.4
Accession Version	NM_014481.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	DNA-(apurinic or apyrimidinic site) lyase 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP363514.1, BC002959.1 and BM795632.1. This sequence is a reference standard in the RefSeqGene project. On Nov 14, 2012 this sequence version replaced NM_014481.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803612.247420.1, SRR1660803.245155.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTGCGCG TGGTGAGCTG GAACATCAAT GGGATTCGGA GACCCCTGCA AGGGGTGGCA 
AATCAGGAAC CCAGCAACTG TGCCGCCGTG GCCGTGGGGC GCATTTTGGA CGAGCTGGAT 
GCGGATATCG TCTGTCTCCA GGAAACCAAA GTGACCAGGG ATGCACTGAC AGAGCCCCTG 
GCTATCGTTG AGGGTTATAA CTCCTATTTC AGCTTCAGCC GCAACCGTAG CGGCTATTCT 
GGTGTAGCCA CCTTCTGTAA GGACAATGCT ACCCCAGTGG CTGCTGAAGA AGGCCTGAGT 
GGCCTGTTTG CCACCCAGAA TGGGGATGTT GGTTGCTATG GAAACATGGA TGAGTTTACC 
CAAGAGGAAC TCCGGGCTCT GGATAGTGAG GGCAGGGCCC TCCTCACACA GCATAAGATC 
CGCACATGGG AAGGTAAGGA GAAGACCTTG ACCCTAATCA ACGTGTACTG CCCCCATGCG 
GACCCTGGGA GGCCTGAGCG GCTAGTCTTT AAGATGCGCT TCTATCGTTT GCTGCAAATC 
CGAGCAGAAG CCCTCCTGGC GGCAGGCAGC CATGTGATCA TTCTGGGTGA CCTGAATACA 
GCCCACCGCC CCATTGACCA CTGGGATGCA GTCAACCTGG AATGCTTTGA AGAGGACCCA 
GGGCGCAAGT GGATGGACAG CTTGCTCAGT AACTTGGGGT GCCAGTCTGC CTCTCATGTA 
GGGCCCTTCA TCGATAGCTA CCGCTGCTTC CAACCAAAGC AGGAGGGGGC CTTCACCTGC 
TGGTCAGCAG TCACTGGCGC CCGCCATCTC AACTATGGCT CCCGGCTTGA CTATGTGCTG 
GGGGACAGGA CCCTGGTCAT AGACACCTTT CAGGCCTCTT TCCTGCTGCC TGAGGTGATG 
GGCTCTGACC ACTGCCCTGT GGGTGCAGTC TTGAGTGTGT CCTCTGTGCC TGCAAAACAG 
TGCCCACCTC TGTGCACCCG CTTCCTCCCT GAGTTTGCAG GCACCCAGCT CAAGATCCTT 
CGCTTCCTAG TTCCTCTCGA ACAAAGTCCT GTGTTGGAGC AGTCGACGCT GCAGCACAAC 
AATCAAACCC GGGTACAGAC ATGCCAAAAC AAAGCCCAAG TGCGCTCAAC CAGGCCTCAG 
CCCAGTCAGG TTGGCTCTAG CAGAGGCCAG AAAAACCTGA AGAGCTACTT TCAGCCCTCC 
CCTAGCTGTC CCCAAGCCTC TCCTGACATA GAGCTGCCTA GCCTACCACT GATGAGCGCC 
CTCATGACCC CGAAGACTCC AGAAGAGAAG GCAGTGGCCA AAGTGGTGAA GGGGCAGGCC 
AAGACTTCAG AAGCCAAAGA TGAGAAGGAG TTACGGACCT CATTCTGGAA GTCTGTGCTG 
GCGGGGCCCT TGCGCACACC CCTCTGTGGG GGCCACAGGG AGCCATGTGT GATGCGTACT 
GTGAAGAAGC CAGGACCCAA CTTGGGCCGC CGCTTCTACA TGTGTGCCAG GCCCCGGGGT 
CCTCCCACTG ACCCCTCCTC CCGGTGCAAC TTCTTCCTCT GGAGCAGGCC CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055296.2
CDS	101..1657
Translation	MLRVVSWNINGIRRPLQGVANQEPSNCAAVAVGRILDELDADIVCLQETKVTRDALTEPLAIVEGYNSYFSFSRNRSGYSGVATFCKDNATPVAAEEGLSGLFATQNGDVGCYGNMDEFTQEELRALDSEGRALLTQHKIRTWEGKEKTLTLINVYCPHADPGRPERLVFKMRFYRLLQIRAEALLAAGSHVIILGDLNTAHRPIDHWDAVNLECFEEDPGRKWMDSLLSNLGCQSASHVGPFIDSYRCFQPKQEGAFTCWSAVTGARHLNYGSRLDYVLGDRTLVIDTFQASFLLPEVMGSDHCPVGAVLSVSSVPAKQCPPLCTRFLPEFAGTQLKILRFLVPLEQSPVLEQSTLQHNNQTRVQTCQNKAQVRSTRPQPSQVGSSRGQKNLKSYFQPSPSCPQASPDIELPSLPLMSALMTPKTPEEKAVAKVVKGQAKTSEAKDEKELRTSFWKSVLAGPLRTPLCGGHREPCVMRTVKKPGPNLGRRFYMCARPRGPPTDPSSRCNFFLWSRPS

Target ORF information:

RefSeq Version	NM_014481.3
Organism	Homo sapiens(human)
Definition	Homo sapiens apurinic/apyrimidinic endodeoxyribonuclease 2 (APEX2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014481.3

ORF Insert Sequence:

ATGTTGCGCG TGGTGAGCTG GAACATCAAT GGGATTCGGA GACCCCTGCA AGGGGTGGCA 
AATCAGGAAC CCAGCAACTG TGCCGCCGTG GCCGTGGGGC GCATTTTGGA CGAGCTGGAT 
GCGGATATCG TCTGTCTCCA GGAAACCAAA GTGACCAGGG ATGCACTGAC AGAGCCCCTG 
GCTATCGTTG AGGGTTATAA CTCCTATTTC AGCTTCAGCC GCAACCGTAG CGGCTATTCT 
GGTGTAGCCA CCTTCTGTAA GGACAATGCT ACCCCAGTGG CTGCTGAAGA AGGCCTGAGT 
GGCCTGTTTG CCACCCAGAA TGGGGATGTT GGTTGCTATG GAAACATGGA TGAGTTTACC 
CAAGAGGAAC TCCGGGCTCT GGATAGTGAG GGCAGGGCCC TCCTCACACA GCATAAGATC 
CGCACATGGG AAGGTAAGGA GAAGACCTTG ACCCTAATCA ACGTGTACTG CCCCCATGCG 
GACCCTGGGA GGCCTGAGCG GCTAGTCTTT AAGATGCGCT TCTATCGTTT GCTGCAAATC 
CGAGCAGAAG CCCTCCTGGC GGCAGGCAGC CATGTGATCA TTCTGGGTGA CCTGAATACA 
GCCCACCGCC CCATTGACCA CTGGGATGCA GTCAACCTGG AATGCTTTGA AGAGGACCCA 
GGGCGCAAGT GGATGGACAG CTTGCTCAGT AACTTGGGGT GCCAGTCTGC CTCTCATGTA 
GGGCCCTTCA TCGATAGCTA CCGCTGCTTC CAACCAAAGC AGGAGGGGGC CTTCACCTGC 
TGGTCAGCAG TCACTGGCGC CCGCCATCTC AACTATGGCT CCCGGCTTGA CTATGTGCTG 
GGGGACAGGA CCCTGGTCAT AGACACCTTT CAGGCCTCTT TCCTGCTGCC TGAGGTGATG 
GGCTCTGACC ACTGCCCTGT GGGTGCAGTC TTGAGTGTGT CCTCTGTGCC TGCAAAACAG 
TGCCCACCTC TGTGCACCCG CTTCCTCCCT GAGTTTGCAG GCACCCAGCT CAAGATCCTT 
CGCTTCCTAG TTCCTCTCGA ACAAAGTCCT GTGTTGGAGC AGTCGACGCT GCAGCACAAC 
AATCAAACCC GGGTACAGAC ATGCCAAAAC AAAGCCCAAG TGCGCTCAAC CAGGCCTCAG 
CCCAGTCAGG TTGGCTCTAG CAGAGGCCAG AAAAACCTGA AGAGCTACTT TCAGCCCTCC 
CCTAGCTGTC CCCAAGCCTC TCCTGACATA GAGCTGCCTA GCCTACCACT GATGAGCGCC 
CTCATGACCC CGAAGACTCC AGAAGAGAAG GCAGTGGCCA AAGTGGTGAA GGGGCAGGCC 
AAGACTTCAG AAGCCAAAGA TGAGAAGGAG TTACGGACCT CATTCTGGAA GTCTGTGCTG 
GCGGGGCCCT TGCGCACACC CCTCTGTGGG GGCCACAGGG AGCCATGTGT GATGCGTACT 
GTGAAGAAGC CAGGACCCAA CTTGGGCCGC CGCTTCTACA TGTGTGCCAG GCCCCGGGGT 
CCTCCCACTG ACCCCTCCTC CCGGTGCAAC TTCTTCCTCT GGAGCAGGCC CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APEX2 ( NM_014481.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055296.2 Homo sapiens apurinic/apyrimidinic endodeoxyribonuclease 2 (APEX2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20076
Clone ID Related Accession (Same CDS sequence)	NM_014481.3 , NM_014481.4
Accession Version	NM_014481.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	DNA-(apurinic or apyrimidinic site) lyase 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP363514.1, BC002959.1, BM795632.1 and AL020991.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_014481.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AB021260.1, SRR1660803.245155.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000374987.4/ ENSP00000364126.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTGCGCG TGGTGAGCTG GAACATCAAT GGGATTCGGA GACCCCTGCA AGGGGTGGCA 
AATCAGGAAC CCAGCAACTG TGCCGCCGTG GCCGTGGGGC GCATTTTGGA CGAGCTGGAT 
GCGGATATCG TCTGTCTCCA GGAAACCAAA GTGACCAGGG ATGCACTGAC AGAGCCCCTG 
GCTATCGTTG AGGGTTATAA CTCCTATTTC AGCTTCAGCC GCAACCGTAG CGGCTATTCT 
GGTGTAGCCA CCTTCTGTAA GGACAATGCT ACCCCAGTGG CTGCTGAAGA AGGCCTGAGT 
GGCCTGTTTG CCACCCAGAA TGGGGATGTT GGTTGCTATG GAAACATGGA TGAGTTTACC 
CAAGAGGAAC TCCGGGCTCT GGATAGTGAG GGCAGGGCCC TCCTCACACA GCATAAGATC 
CGCACATGGG AAGGTAAGGA GAAGACCTTG ACCCTAATCA ACGTGTACTG CCCCCATGCG 
GACCCTGGGA GGCCTGAGCG GCTAGTCTTT AAGATGCGCT TCTATCGTTT GCTGCAAATC 
CGAGCAGAAG CCCTCCTGGC GGCAGGCAGC CATGTGATCA TTCTGGGTGA CCTGAATACA 
GCCCACCGCC CCATTGACCA CTGGGATGCA GTCAACCTGG AATGCTTTGA AGAGGACCCA 
GGGCGCAAGT GGATGGACAG CTTGCTCAGT AACTTGGGGT GCCAGTCTGC CTCTCATGTA 
GGGCCCTTCA TCGATAGCTA CCGCTGCTTC CAACCAAAGC AGGAGGGGGC CTTCACCTGC 
TGGTCAGCAG TCACTGGCGC CCGCCATCTC AACTATGGCT CCCGGCTTGA CTATGTGCTG 
GGGGACAGGA CCCTGGTCAT AGACACCTTT CAGGCCTCTT TCCTGCTGCC TGAGGTGATG 
GGCTCTGACC ACTGCCCTGT GGGTGCAGTC TTGAGTGTGT CCTCTGTGCC TGCAAAACAG 
TGCCCACCTC TGTGCACCCG CTTCCTCCCT GAGTTTGCAG GCACCCAGCT CAAGATCCTT 
CGCTTCCTAG TTCCTCTCGA ACAAAGTCCT GTGTTGGAGC AGTCGACGCT GCAGCACAAC 
AATCAAACCC GGGTACAGAC ATGCCAAAAC AAAGCCCAAG TGCGCTCAAC CAGGCCTCAG 
CCCAGTCAGG TTGGCTCTAG CAGAGGCCAG AAAAACCTGA AGAGCTACTT TCAGCCCTCC 
CCTAGCTGTC CCCAAGCCTC TCCTGACATA GAGCTGCCTA GCCTACCACT GATGAGCGCC 
CTCATGACCC CGAAGACTCC AGAAGAGAAG GCAGTGGCCA AAGTGGTGAA GGGGCAGGCC 
AAGACTTCAG AAGCCAAAGA TGAGAAGGAG TTACGGACCT CATTCTGGAA GTCTGTGCTG 
GCGGGGCCCT TGCGCACACC CCTCTGTGGG GGCCACAGGG AGCCATGTGT GATGCGTACT 
GTGAAGAAGC CAGGACCCAA CTTGGGCCGC CGCTTCTACA TGTGTGCCAG GCCCCGGGGT 
CCTCCCACTG ACCCCTCCTC CCGGTGCAAC TTCTTCCTCT GGAGCAGGCC CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055296.2
CDS	61..1617
Translation	MLRVVSWNINGIRRPLQGVANQEPSNCAAVAVGRILDELDADIVCLQETKVTRDALTEPLAIVEGYNSYFSFSRNRSGYSGVATFCKDNATPVAAEEGLSGLFATQNGDVGCYGNMDEFTQEELRALDSEGRALLTQHKIRTWEGKEKTLTLINVYCPHADPGRPERLVFKMRFYRLLQIRAEALLAAGSHVIILGDLNTAHRPIDHWDAVNLECFEEDPGRKWMDSLLSNLGCQSASHVGPFIDSYRCFQPKQEGAFTCWSAVTGARHLNYGSRLDYVLGDRTLVIDTFQASFLLPEVMGSDHCPVGAVLSVSSVPAKQCPPLCTRFLPEFAGTQLKILRFLVPLEQSPVLEQSTLQHNNQTRVQTCQNKAQVRSTRPQPSQVGSSRGQKNLKSYFQPSPSCPQASPDIELPSLPLMSALMTPKTPEEKAVAKVVKGQAKTSEAKDEKELRTSFWKSVLAGPLRTPLCGGHREPCVMRTVKKPGPNLGRRFYMCARPRGPPTDPSSRCNFFLWSRPS

Target ORF information:

RefSeq Version	NM_014481.4
Organism	Homo sapiens(human)
Definition	Homo sapiens apurinic/apyrimidinic endodeoxyribonuclease 2 (APEX2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014481.4

ORF Insert Sequence:

ATGTTGCGCG TGGTGAGCTG GAACATCAAT GGGATTCGGA GACCCCTGCA AGGGGTGGCA 
AATCAGGAAC CCAGCAACTG TGCCGCCGTG GCCGTGGGGC GCATTTTGGA CGAGCTGGAT 
GCGGATATCG TCTGTCTCCA GGAAACCAAA GTGACCAGGG ATGCACTGAC AGAGCCCCTG 
GCTATCGTTG AGGGTTATAA CTCCTATTTC AGCTTCAGCC GCAACCGTAG CGGCTATTCT 
GGTGTAGCCA CCTTCTGTAA GGACAATGCT ACCCCAGTGG CTGCTGAAGA AGGCCTGAGT 
GGCCTGTTTG CCACCCAGAA TGGGGATGTT GGTTGCTATG GAAACATGGA TGAGTTTACC 
CAAGAGGAAC TCCGGGCTCT GGATAGTGAG GGCAGGGCCC TCCTCACACA GCATAAGATC 
CGCACATGGG AAGGTAAGGA GAAGACCTTG ACCCTAATCA ACGTGTACTG CCCCCATGCG 
GACCCTGGGA GGCCTGAGCG GCTAGTCTTT AAGATGCGCT TCTATCGTTT GCTGCAAATC 
CGAGCAGAAG CCCTCCTGGC GGCAGGCAGC CATGTGATCA TTCTGGGTGA CCTGAATACA 
GCCCACCGCC CCATTGACCA CTGGGATGCA GTCAACCTGG AATGCTTTGA AGAGGACCCA 
GGGCGCAAGT GGATGGACAG CTTGCTCAGT AACTTGGGGT GCCAGTCTGC CTCTCATGTA 
GGGCCCTTCA TCGATAGCTA CCGCTGCTTC CAACCAAAGC AGGAGGGGGC CTTCACCTGC 
TGGTCAGCAG TCACTGGCGC CCGCCATCTC AACTATGGCT CCCGGCTTGA CTATGTGCTG 
GGGGACAGGA CCCTGGTCAT AGACACCTTT CAGGCCTCTT TCCTGCTGCC TGAGGTGATG 
GGCTCTGACC ACTGCCCTGT GGGTGCAGTC TTGAGTGTGT CCTCTGTGCC TGCAAAACAG 
TGCCCACCTC TGTGCACCCG CTTCCTCCCT GAGTTTGCAG GCACCCAGCT CAAGATCCTT 
CGCTTCCTAG TTCCTCTCGA ACAAAGTCCT GTGTTGGAGC AGTCGACGCT GCAGCACAAC 
AATCAAACCC GGGTACAGAC ATGCCAAAAC AAAGCCCAAG TGCGCTCAAC CAGGCCTCAG 
CCCAGTCAGG TTGGCTCTAG CAGAGGCCAG AAAAACCTGA AGAGCTACTT TCAGCCCTCC 
CCTAGCTGTC CCCAAGCCTC TCCTGACATA GAGCTGCCTA GCCTACCACT GATGAGCGCC 
CTCATGACCC CGAAGACTCC AGAAGAGAAG GCAGTGGCCA AAGTGGTGAA GGGGCAGGCC 
AAGACTTCAG AAGCCAAAGA TGAGAAGGAG TTACGGACCT CATTCTGGAA GTCTGTGCTG 
GCGGGGCCCT TGCGCACACC CCTCTGTGGG GGCCACAGGG AGCCATGTGT GATGCGTACT 
GTGAAGAAGC CAGGACCCAA CTTGGGCCGC CGCTTCTACA TGTGTGCCAG GCCCCGGGGT 
CCTCCCACTG ACCCCTCCTC CCGGTGCAAC TTCTTCCTCT GGAGCAGGCC CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APEX2 ( NM_001271748.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258677.1 Homo sapiens apurinic/apyrimidinic endodeoxyribonuclease 2 (APEX2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09706
Clone ID Related Accession (Same CDS sequence)	NM_001271748.1 , NM_001271748.2
Accession Version	NM_001271748.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-17
Organism	Homo sapiens(human)
Product	DNA-(apurinic or apyrimidinic site) lyase 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP363514.1, AK316186.1, AK301555.1 and BM795632.1. Transcript Variant: This variant (2) lacks a 5' exon compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a weak Kozak sequence and a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG. The encoded protein (isoform 2) has a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.206499.1, SRR1163658.91309.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGCTTCT ATCGTTTGCT GCAAATCCGA GCAGAAGCCC TCCTGGCGGC AGGCAGCCAT 
GTGATCATTC TGGGTGACCT GAATACAGCC CACCGCCCCA TTGACCACTG GGATGCAGTC 
AACCTGGAAT GCTTTGAAGA GGACCCAGGG CGCAAGTGGA TGGACAGCTT GCTCAGTAAC 
TTGGGGTGCC AGTCTGCCTC TCATGTAGGG CCCTTCATCG ATAGCTACCG CTGCTTCCAA 
CCAAAGCAGG AGGGGGCCTT CACCTGCTGG TCAGCAGTCA CTGGCGCCCG CCATCTCAAC 
TATGGCTCCC GGCTTGACTA TGTGCTGGGG GACAGGACCC TGGTCATAGA CACCTTTCAG 
GCCTCTTTCC TGCTGCCTGA GGTGATGGGC TCTGACCACT GCCCTGTGGG TGCAGTCTTG 
AGTGTGTCCT CTGTGCCTGC AAAACAGTGC CCACCTCTGT GCACCCGCTT CCTCCCTGAG 
TTTGCAGGCA CCCAGCTCAA GATCCTTCGC TTCCTAGTTC CTCTCGAACA AAGTCCTGTG 
TTGGAGCAGT CGACGCTGCA GCACAACAAT CAAACCCGGG TACAGACATG CCAAAACAAA 
GCCCAAGTGC GCTCAACCAG GCCTCAGCCC AGTCAGGTTG GCTCTAGCAG AGGCCAGAAA 
AACCTGAAGA GCTACTTTCA GCCCTCCCCT AGCTGTCCCC AAGCCTCTCC TGACATAGAG 
CTGCCTAGCC TACCACTGAT GAGCGCCCTC ATGACCCCGA AGACTCCAGA AGAGAAGGCA 
GTGGCCAAAG TGGTGAAGGG GCAGGCCAAG ACTTCAGAAG CCAAAGATGA GAAGGAGTTA 
CGGACCTCAT TCTGGAAGTC TGTGCTGGCG GGGCCCTTGC GCACACCCCT CTGTGGGGGC 
CACAGGGAGC CATGTGTGAT GCGTACTGTG AAGAAGCCAG GACCCAACTT GGGCCGCCGC 
TTCTACATGT GTGCCAGGCC CCGGGGTCCT CCCACTGACC CCTCCTCCCG GTGCAACTTC 
TTCCTCTGGA GCAGGCCCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258677.1
CDS	433..1476
Translation	MRFYRLLQIRAEALLAAGSHVIILGDLNTAHRPIDHWDAVNLECFEEDPGRKWMDSLLSNLGCQSASHVGPFIDSYRCFQPKQEGAFTCWSAVTGARHLNYGSRLDYVLGDRTLVIDTFQASFLLPEVMGSDHCPVGAVLSVSSVPAKQCPPLCTRFLPEFAGTQLKILRFLVPLEQSPVLEQSTLQHNNQTRVQTCQNKAQVRSTRPQPSQVGSSRGQKNLKSYFQPSPSCPQASPDIELPSLPLMSALMTPKTPEEKAVAKVVKGQAKTSEAKDEKELRTSFWKSVLAGPLRTPLCGGHREPCVMRTVKKPGPNLGRRFYMCARPRGPPTDPSSRCNFFLWSRPS

Target ORF information:

RefSeq Version	NM_001271748.1
Organism	Homo sapiens(human)
Definition	Homo sapiens apurinic/apyrimidinic endodeoxyribonuclease 2 (APEX2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271748.1

ORF Insert Sequence:

ATGCGCTTCT ATCGTTTGCT GCAAATCCGA GCAGAAGCCC TCCTGGCGGC AGGCAGCCAT 
GTGATCATTC TGGGTGACCT GAATACAGCC CACCGCCCCA TTGACCACTG GGATGCAGTC 
AACCTGGAAT GCTTTGAAGA GGACCCAGGG CGCAAGTGGA TGGACAGCTT GCTCAGTAAC 
TTGGGGTGCC AGTCTGCCTC TCATGTAGGG CCCTTCATCG ATAGCTACCG CTGCTTCCAA 
CCAAAGCAGG AGGGGGCCTT CACCTGCTGG TCAGCAGTCA CTGGCGCCCG CCATCTCAAC 
TATGGCTCCC GGCTTGACTA TGTGCTGGGG GACAGGACCC TGGTCATAGA CACCTTTCAG 
GCCTCTTTCC TGCTGCCTGA GGTGATGGGC TCTGACCACT GCCCTGTGGG TGCAGTCTTG 
AGTGTGTCCT CTGTGCCTGC AAAACAGTGC CCACCTCTGT GCACCCGCTT CCTCCCTGAG 
TTTGCAGGCA CCCAGCTCAA GATCCTTCGC TTCCTAGTTC CTCTCGAACA AAGTCCTGTG 
TTGGAGCAGT CGACGCTGCA GCACAACAAT CAAACCCGGG TACAGACATG CCAAAACAAA 
GCCCAAGTGC GCTCAACCAG GCCTCAGCCC AGTCAGGTTG GCTCTAGCAG AGGCCAGAAA 
AACCTGAAGA GCTACTTTCA GCCCTCCCCT AGCTGTCCCC AAGCCTCTCC TGACATAGAG 
CTGCCTAGCC TACCACTGAT GAGCGCCCTC ATGACCCCGA AGACTCCAGA AGAGAAGGCA 
GTGGCCAAAG TGGTGAAGGG GCAGGCCAAG ACTTCAGAAG CCAAAGATGA GAAGGAGTTA 
CGGACCTCAT TCTGGAAGTC TGTGCTGGCG GGGCCCTTGC GCACACCCCT CTGTGGGGGC 
CACAGGGAGC CATGTGTGAT GCGTACTGTG AAGAAGCCAG GACCCAACTT GGGCCGCCGC 
TTCTACATGT GTGCCAGGCC CCGGGGTCCT CCCACTGACC CCTCCTCCCG GTGCAACTTC 
TTCCTCTGGA GCAGGCCCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APEX2 ( NM_001271748.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258677.1 Homo sapiens apurinic/apyrimidinic endodeoxyribonuclease 2 (APEX2), transcript variant 2, mRNA.

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CloneID	OHu09706
Clone ID Related Accession (Same CDS sequence)	NM_001271748.1 , NM_001271748.2
Accession Version	NM_001271748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	DNA-(apurinic or apyrimidinic site) lyase 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP363514.1, AK316186.1, AK301555.1, BM795632.1 and AL020991.1. On May 31, 2019 this sequence version replaced NM_001271748.1. Transcript Variant: This variant (2) lacks a 5' exon compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a weak Kozak sequence and a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG. The encoded protein (isoform 2) has a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.206499.1, AK301555.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGCTTCT ATCGTTTGCT GCAAATCCGA GCAGAAGCCC TCCTGGCGGC AGGCAGCCAT 
GTGATCATTC TGGGTGACCT GAATACAGCC CACCGCCCCA TTGACCACTG GGATGCAGTC 
AACCTGGAAT GCTTTGAAGA GGACCCAGGG CGCAAGTGGA TGGACAGCTT GCTCAGTAAC 
TTGGGGTGCC AGTCTGCCTC TCATGTAGGG CCCTTCATCG ATAGCTACCG CTGCTTCCAA 
CCAAAGCAGG AGGGGGCCTT CACCTGCTGG TCAGCAGTCA CTGGCGCCCG CCATCTCAAC 
TATGGCTCCC GGCTTGACTA TGTGCTGGGG GACAGGACCC TGGTCATAGA CACCTTTCAG 
GCCTCTTTCC TGCTGCCTGA GGTGATGGGC TCTGACCACT GCCCTGTGGG TGCAGTCTTG 
AGTGTGTCCT CTGTGCCTGC AAAACAGTGC CCACCTCTGT GCACCCGCTT CCTCCCTGAG 
TTTGCAGGCA CCCAGCTCAA GATCCTTCGC TTCCTAGTTC CTCTCGAACA AAGTCCTGTG 
TTGGAGCAGT CGACGCTGCA GCACAACAAT CAAACCCGGG TACAGACATG CCAAAACAAA 
GCCCAAGTGC GCTCAACCAG GCCTCAGCCC AGTCAGGTTG GCTCTAGCAG AGGCCAGAAA 
AACCTGAAGA GCTACTTTCA GCCCTCCCCT AGCTGTCCCC AAGCCTCTCC TGACATAGAG 
CTGCCTAGCC TACCACTGAT GAGCGCCCTC ATGACCCCGA AGACTCCAGA AGAGAAGGCA 
GTGGCCAAAG TGGTGAAGGG GCAGGCCAAG ACTTCAGAAG CCAAAGATGA GAAGGAGTTA 
CGGACCTCAT TCTGGAAGTC TGTGCTGGCG GGGCCCTTGC GCACACCCCT CTGTGGGGGC 
CACAGGGAGC CATGTGTGAT GCGTACTGTG AAGAAGCCAG GACCCAACTT GGGCCGCCGC 
TTCTACATGT GTGCCAGGCC CCGGGGTCCT CCCACTGACC CCTCCTCCCG GTGCAACTTC 
TTCCTCTGGA GCAGGCCCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258677.1
CDS	393..1436
Translation	MRFYRLLQIRAEALLAAGSHVIILGDLNTAHRPIDHWDAVNLECFEEDPGRKWMDSLLSNLGCQSASHVGPFIDSYRCFQPKQEGAFTCWSAVTGARHLNYGSRLDYVLGDRTLVIDTFQASFLLPEVMGSDHCPVGAVLSVSSVPAKQCPPLCTRFLPEFAGTQLKILRFLVPLEQSPVLEQSTLQHNNQTRVQTCQNKAQVRSTRPQPSQVGSSRGQKNLKSYFQPSPSCPQASPDIELPSLPLMSALMTPKTPEEKAVAKVVKGQAKTSEAKDEKELRTSFWKSVLAGPLRTPLCGGHREPCVMRTVKKPGPNLGRRFYMCARPRGPPTDPSSRCNFFLWSRPS

Target ORF information:

RefSeq Version	NM_001271748.2
Organism	Homo sapiens(human)
Definition	Homo sapiens apurinic/apyrimidinic endodeoxyribonuclease 2 (APEX2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271748.2

ORF Insert Sequence:

ATGCGCTTCT ATCGTTTGCT GCAAATCCGA GCAGAAGCCC TCCTGGCGGC AGGCAGCCAT 
GTGATCATTC TGGGTGACCT GAATACAGCC CACCGCCCCA TTGACCACTG GGATGCAGTC 
AACCTGGAAT GCTTTGAAGA GGACCCAGGG CGCAAGTGGA TGGACAGCTT GCTCAGTAAC 
TTGGGGTGCC AGTCTGCCTC TCATGTAGGG CCCTTCATCG ATAGCTACCG CTGCTTCCAA 
CCAAAGCAGG AGGGGGCCTT CACCTGCTGG TCAGCAGTCA CTGGCGCCCG CCATCTCAAC 
TATGGCTCCC GGCTTGACTA TGTGCTGGGG GACAGGACCC TGGTCATAGA CACCTTTCAG 
GCCTCTTTCC TGCTGCCTGA GGTGATGGGC TCTGACCACT GCCCTGTGGG TGCAGTCTTG 
AGTGTGTCCT CTGTGCCTGC AAAACAGTGC CCACCTCTGT GCACCCGCTT CCTCCCTGAG 
TTTGCAGGCA CCCAGCTCAA GATCCTTCGC TTCCTAGTTC CTCTCGAACA AAGTCCTGTG 
TTGGAGCAGT CGACGCTGCA GCACAACAAT CAAACCCGGG TACAGACATG CCAAAACAAA 
GCCCAAGTGC GCTCAACCAG GCCTCAGCCC AGTCAGGTTG GCTCTAGCAG AGGCCAGAAA 
AACCTGAAGA GCTACTTTCA GCCCTCCCCT AGCTGTCCCC AAGCCTCTCC TGACATAGAG 
CTGCCTAGCC TACCACTGAT GAGCGCCCTC ATGACCCCGA AGACTCCAGA AGAGAAGGCA 
GTGGCCAAAG TGGTGAAGGG GCAGGCCAAG ACTTCAGAAG CCAAAGATGA GAAGGAGTTA 
CGGACCTCAT TCTGGAAGTC TGTGCTGGCG GGGCCCTTGC GCACACCCCT CTGTGGGGGC 
CACAGGGAGC CATGTGTGAT GCGTACTGTG AAGAAGCCAG GACCCAACTT GGGCCGCCGC 
TTCTACATGT GTGCCAGGCC CCGGGGTCCT CCCACTGACC CCTCCTCCCG GTGCAACTTC 
TTCCTCTGGA GCAGGCCCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of PCNA-dependent stimulation of 3'-phosphodiesterase and 3'-5' exonuclease activities of human Ape2 in repair of oxidative DNA damage.
Nucleic acids research37(13)4247-55(2009 Jul)
Burkovics P,Hajd? I,Szukacsov V,Unk I,Haracska L

Human Ape2 protein has a 3'-5' exonuclease activity that acts preferentially on mismatched base pairs.
Nucleic acids research34(9)2508-15(2006)
Burkovics P,Szukacsov V,Unk I,Haracska L

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APEX2 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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