DIMT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DIMT1
Entrez Gene ID	27292
Full Name	DIM1 dimethyladenosine transferase 1 homolog
Synonyms	DIM1,DIMT1L,HSA9761,HUSSY5
General protein information	Preferred Names DIM1 dimethyladenosine transferase 1 homolog Names probable dimethyladenosine transferase 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase 18S rRNA dimethylase DIM1 dimethyladenosine transferase 1-like S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase dimethyladenosine transferase probable 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase probable 18S rRNA dimethylase probable S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q12.1
Summary	The protein encoded by this gene is a methyltransferase that is responsible for dimethylation of adjacent adenosines near the 18S rRNA decoding site. The encoded protein is essential for ribosome biogenesis, although its catalytic activity is not involved in the process. The yeast ortholog of this protein functions in the cytoplasm while this protein functions in the nucleus. [provided by RefSeq, Jan 2017].
Disorder MIM:	612499

mRNA and Protein(s)

mRNA	Protein	Name
XM_011543323.2	XP_011541625.1	probable dimethyladenosine transferase isoform X1
NM_014473.3	NP_055288.1	probable dimethyladenosine transferase isoform 1
NM_001348076.2	NP_001335005.1	probable dimethyladenosine transferase isoform 2
NM_001348077.1	NP_001335006.1	probable dimethyladenosine transferase isoform 3
NM_014473.2	NP_055288.1	probable dimethyladenosine transferase
NM_001348076.1	NP_001335005.1	probable dimethyladenosine transferase isoform 2
NM_001348077.2	NP_001335006.1	probable dimethyladenosine transferase isoform 3
NM_014473.4	NP_055288.1	probable dimethyladenosine transferase isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIMT1	NP_055288.1
Mus musculus (house mouse)	Dimt1	NP_079723.1
Eremothecium gossypii	AGOS_ACR026W	NP_983429.1
Arabidopsis thaliana (thale cress)	AT2G47420	NP_182264.1
Saccharomyces cerevisiae (baker's yeast)	DIM1	NP_015057.1
Pan troglodytes (chimpanzee)	DIMT1	XP_517743.2
Caenorhabditis elegans (roundworm)	E02H1.1	NP_496061.2
Neurospora crassa	NCU08968	XP_960790.1
Oryza sativa (rice)	Os10g0183900	NP_001064257.1
Bos taurus (cattle)	DIMT1	NP_001039501.1
Rattus norvegicus (Norway rat)	Dimt1	NP_001099878.2
Gallus gallus (chicken)	DIMT1	XP_424746.3
Danio rerio (zebrafish)	dimt1l	NP_001003556.1
Drosophila melanogaster (fruit fly)	CG11837	NP_651660.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004465	XP_313763.2
Kluyveromyces lactis	KLLA0D00594g	XP_453098.1
Schizosaccharomyces pombe (fission yeast)	SPBC336.02	NP_596122.1
Magnaporthe oryzae (rice blast fungus)	MGG_17491	XP_003718855.1
Xenopus tropicalis (tropical clawed frog)	dimt1	NP_001116194.1

Gene Ontology
Bibliography

Related articles in PubMed

DIMT1 overexpression correlates with progression and prognosis in gastric carcinoma.
Liu G, Peng X, Cai Y, Cheng A, Zha L, Wang Z
Human pathology7035-42(2017 Dec)

Hypoxia-inducible microRNA-210 regulates the DIMT1-IRF4 oncogenic axis in multiple myeloma.
Ikeda S, Kitadate A, Abe F, Saitoh H, Michishita Y, Hatano Y, Kawabata Y, Kitabayashi A, Teshima K, Kume M, Takahashi N, Tagawa H
Cancer science108(4)641-652(2017 Apr)

The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis.
Zorbas C, Nicolas E, Wacheul L, Huvelle E, Heurgu?-Hamard V, Lafontaine DL
Molecular biology of the cell26(11)2080-95(2015 Jun)

Epigenetic silencing of miR-210 increases the proliferation of gastric epithelium during chronic Helicobacter pylori infection.
Kiga K, Mimuro H, Suzuki M, Shinozaki-Ushiku A, Kobayashi T, Sanada T, Kim M, Ogawa M, Iwasaki YW, Kayo H, Fukuda-Yuzawa Y, Yashiro M, Fukayama M, Fukao T, Sasakawa C
Nature communications54497(2014 Sep)

Characterization of 16 novel human genes showing high similarity to yeast sequences.
Stanchi F, Bertocco E, Toppo S, Dioguardi R, Simionati B, Cannata N, Zimbello R, Lanfranchi G, Valle G
Yeast (Chichester, England)18(1)69-80(2001 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies show that miroRNA miR-210 acts as a tumor suppressor in hypoxic conditions by downregulating the 18S rRNA base methyltransferase (DIMT1)-interferon regulatory factor 4 (IRF4) axis.
Title: Hypoxia-inducible microRNA-210 regulates the DIMT1-IRF4 oncogenic axis in multiple myeloma.

DIMT1L and WBSCR22-TRMT112 are required for distinct pre-rRNA processing reactions leading to synthesis of 18S rRNA. Ribosome biogenesis requires the presence of the modification enzyme rather than its RNA-modifying catalytic activity.
Title: The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis.

Epigenetic silencing of miR-210 increases the proliferation of gastric epithelium during chronic Helicobacter pylori infection by activating STMN1 and DIMT1.
Title: Epigenetic silencing of miR-210 increases the proliferation of gastric epithelium during chronic Helicobacter pylori infection.

The following DIMT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIMT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06406	NM_014473.4 Latest version!	Homo sapiens DIMT1 rRNA methyltransferase and ribosome maturation factor (DIMT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu67177	NM_001348077.2 Latest version!	Homo sapiens DIMT1 rRNA methyltransferase and ribosome maturation factor (DIMT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu67177	XM_011543323.2 Latest version!	Homo sapiens DIM1 dimethyladenosine transferase 1 homolog (DIMT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu102340	NM_001348076.2 Latest version!	Homo sapiens DIMT1 rRNA methyltransferase and ribosome maturation factor (DIMT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu06406	NM_014473.2	Homo sapiens DIM1 dimethyladenosine transferase 1 homolog (DIMT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu102340	NM_001348076.1	Homo sapiens DIMT1 rRNA methyltransferase and ribosome maturation factor (DIMT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu67177	NM_001348077.1	Homo sapiens DIMT1 rRNA methyltransferase and ribosome maturation factor (DIMT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu06406	NM_014473.3	Homo sapiens DIM1 dimethyladenosine transferase 1 homolog (DIMT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DIMT1 ( NM_014473.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055288.1 Homo sapiens DIM1 dimethyladenosine transferase 1 homolog (DIMT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06406
Clone ID Related Accession (Same CDS sequence)	NM_014473.3 , NM_014473.2 , NM_014473.4
Accession Version	NM_014473.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	probable dimethyladenosine transferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC398380.1, AK292723.1, AC016637.7 and BQ009563.1. On Feb 8, 2017 this sequence version replaced NM_014473.2. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803616.29635.1, SRR1660805.29519.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGAAGG TCAAGTCGGG GGCCATCGGC CGCCGCCGCG GGCGGCAGGA GCAGCGCCGG 
GAGCTGAAGA GCGCTGGAGG ACTCATGTTC AACACGGGGA TTGGGCAGCA CATTTTGAAA 
AATCCTCTCA TTATTAACAG CATTATCGAT AAGGCTGCCT TAAGACCAAC TGATGTAGTG 
CTGGAAGTTG GACCTGGAAC TGGCAACATG ACTGTAAAGT TGTTAGAAAA GGCAAAAAAG 
GTTGTTGCTT GTGAACTTGA CCCAAGGCTA GTAGCTGAAC TTCACAAAAG AGTTCAGGGC 
ACGCCTGTGG CCAGCAAACT TCAAGTACTG GTGGGTGATG TGCTGAAAAC AGATTTGCCA 
TTCTTTGATA CTTGTGTGGC AAATTTGCCT TATCAGATCT CTTCACCTTT TGTCTTCAAG 
CTGTTGCTAC ATCGACCTTT TTTCAGGTGT GCTATACTTA TGTTTCAAAG AGAATTTGCC 
CTCCGACTGG TTGCAAAACC TGGAGATAAG TTATACTGCA GACTCTCAAT TAATACACAG 
CTGTTGGCAC GTGTGGACCA TCTAATGAAA GTGGGAAAGA ATAACTTCAG ACCACCGCCC 
AAGGTGGAAT CCAGTGTTGT AAGGATAGAA CCTAAGAATC CACCACCACC CATCAATTTT 
CAGGAATGGG ATGGTCTAGT AAGGATAACC TTTGTTAGGA AAAACAAGAC ACTCTCTGCT 
GCATTTAAAT CAAGTGCAGT GCAACAACTC TTGGAAAAAA ACTACAGAAT TCACTGTTCA 
GTCCATAATA TTATAATACC AGAAGATTTC AGCATAGCAG ATAAAATACA GCAAATCCTA 
ACCAGCACAG GTTTTAGTGA CAAACGGGCC CGTTCCATGG ACATAGATGA CTTCATCAGA 
TTGCTACATG GATTCAACGC AGAAGGTATT CATTTTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055288.1
CDS	189..1130
Translation	MPKVKSGAIGRRRGRQEQRRELKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCVANLPYQISSPFVFKLLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIIIPEDFSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS

Target ORF information:

RefSeq Version	NM_014473.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DIM1 dimethyladenosine transferase 1 homolog (DIMT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014473.3

ORF Insert Sequence:

ATGCCGAAGG TCAAGTCGGG GGCCATCGGC CGCCGCCGCG GGCGGCAGGA GCAGCGCCGG 
GAGCTGAAGA GCGCTGGAGG ACTCATGTTC AACACGGGGA TTGGGCAGCA CATTTTGAAA 
AATCCTCTCA TTATTAACAG CATTATCGAT AAGGCTGCCT TAAGACCAAC TGATGTAGTG 
CTGGAAGTTG GACCTGGAAC TGGCAACATG ACTGTAAAGT TGTTAGAAAA GGCAAAAAAG 
GTTGTTGCTT GTGAACTTGA CCCAAGGCTA GTAGCTGAAC TTCACAAAAG AGTTCAGGGC 
ACGCCTGTGG CCAGCAAACT TCAAGTACTG GTGGGTGATG TGCTGAAAAC AGATTTGCCA 
TTCTTTGATA CTTGTGTGGC AAATTTGCCT TATCAGATCT CTTCACCTTT TGTCTTCAAG 
CTGTTGCTAC ATCGACCTTT TTTCAGGTGT GCTATACTTA TGTTTCAAAG AGAATTTGCC 
CTCCGACTGG TTGCAAAACC TGGAGATAAG TTATACTGCA GACTCTCAAT TAATACACAG 
CTGTTGGCAC GTGTGGACCA TCTAATGAAA GTGGGAAAGA ATAACTTCAG ACCACCGCCC 
AAGGTGGAAT CCAGTGTTGT AAGGATAGAA CCTAAGAATC CACCACCACC CATCAATTTT 
CAGGAATGGG ATGGTCTAGT AAGGATAACC TTTGTTAGGA AAAACAAGAC ACTCTCTGCT 
GCATTTAAAT CAAGTGCAGT GCAACAACTC TTGGAAAAAA ACTACAGAAT TCACTGTTCA 
GTCCATAATA TTATAATACC AGAAGATTTC AGCATAGCAG ATAAAATACA GCAAATCCTA 
ACCAGCACAG GTTTTAGTGA CAAACGGGCC CGTTCCATGG ACATAGATGA CTTCATCAGA 
TTGCTACATG GATTCAACGC AGAAGGTATT CATTTTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIMT1 ( NM_014473.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055288.1 Homo sapiens DIM1 dimethyladenosine transferase 1 homolog (DIMT1), mRNA.

Price & Availability↑

CloneID	OHu06406
Clone ID Related Accession (Same CDS sequence)	NM_014473.3 , NM_014473.2 , NM_014473.4
Accession Version	NM_014473.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-03
Organism	Homo sapiens(Human)
Product	probable dimethyladenosine transferase
Comment

ATGCCGAAGG TCAAGTCGGG GGCCATCGGC CGCCGCCGCG GGCGGCAGGA GCAGCGCCGG 
GAGCTGAAGA GCGCTGGAGG ACTCATGTTC AACACGGGGA TTGGGCAGCA CATTTTGAAA 
AATCCTCTCA TTATTAACAG CATTATCGAT AAGGCTGCCT TAAGACCAAC TGATGTAGTG 
CTGGAAGTTG GACCTGGAAC TGGCAACATG ACTGTAAAGT TGTTAGAAAA GGCAAAAAAG 
GTTGTTGCTT GTGAACTTGA CCCAAGGCTA GTAGCTGAAC TTCACAAAAG AGTTCAGGGC 
ACGCCTGTGG CCAGCAAACT TCAAGTACTG GTGGGTGATG TGCTGAAAAC AGATTTGCCA 
TTCTTTGATA CTTGTGTGGC AAATTTGCCT TATCAGATCT CTTCACCTTT TGTCTTCAAG 
CTGTTGCTAC ATCGACCTTT TTTCAGGTGT GCTATACTTA TGTTTCAAAG AGAATTTGCC 
CTCCGACTGG TTGCAAAACC TGGAGATAAG TTATACTGCA GACTCTCAAT TAATACACAG 
CTGTTGGCAC GTGTGGACCA TCTAATGAAA GTGGGAAAGA ATAACTTCAG ACCACCGCCC 
AAGGTGGAAT CCAGTGTTGT AAGGATAGAA CCTAAGAATC CACCACCACC CATCAATTTT 
CAGGAATGGG ATGGTCTAGT AAGGATAACC TTTGTTAGGA AAAACAAGAC ACTCTCTGCT 
GCATTTAAAT CAAGTGCAGT GCAACAACTC TTGGAAAAAA ACTACAGAAT TCACTGTTCA 
GTCCATAATA TTATAATACC AGAAGATTTC AGCATAGCAG ATAAAATACA GCAAATCCTA 
ACCAGCACAG GTTTTAGTGA CAAACGGGCC CGTTCCATGG ACATAGATGA CTTCATCAGA 
TTGCTACATG GATTCAACGC AGAAGGTATT CATTTTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055288.1
Translation	MPKVKSGAIGRRRGRQEQRRELKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCVANLPYQISSPFVFKLLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIIIPEDFSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS

Target ORF information:

RefSeq Version	NM_014473.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens DIM1 dimethyladenosine transferase 1 homolog (DIMT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014473.2

ORF Insert Sequence:

ATGCCGAAGG TCAAGTCGGG GGCCATCGGC CGCCGCCGCG GGCGGCAGGA GCAGCGCCGG 
GAGCTGAAGA GCGCTGGAGG ACTCATGTTC AACACGGGGA TTGGGCAGCA CATTTTGAAA 
AATCCTCTCA TTATTAACAG CATTATCGAT AAGGCTGCCT TAAGACCAAC TGATGTAGTG 
CTGGAAGTTG GACCTGGAAC TGGCAACATG ACTGTAAAGT TGTTAGAAAA GGCAAAAAAG 
GTTGTTGCTT GTGAACTTGA CCCAAGGCTA GTAGCTGAAC TTCACAAAAG AGTTCAGGGC 
ACGCCTGTGG CCAGCAAACT TCAAGTACTG GTGGGTGATG TGCTGAAAAC AGATTTGCCA 
TTCTTTGATA CTTGTGTGGC AAATTTGCCT TATCAGATCT CTTCACCTTT TGTCTTCAAG 
CTGTTGCTAC ATCGACCTTT TTTCAGGTGT GCTATACTTA TGTTTCAAAG AGAATTTGCC 
CTCCGACTGG TTGCAAAACC TGGAGATAAG TTATACTGCA GACTCTCAAT TAATACACAG 
CTGTTGGCAC GTGTGGACCA TCTAATGAAA GTGGGAAAGA ATAACTTCAG ACCACCGCCC 
AAGGTGGAAT CCAGTGTTGT AAGGATAGAA CCTAAGAATC CACCACCACC CATCAATTTT 
CAGGAATGGG ATGGTCTAGT AAGGATAACC TTTGTTAGGA AAAACAAGAC ACTCTCTGCT 
GCATTTAAAT CAAGTGCAGT GCAACAACTC TTGGAAAAAA ACTACAGAAT TCACTGTTCA 
GTCCATAATA TTATAATACC AGAAGATTTC AGCATAGCAG ATAAAATACA GCAAATCCTA 
ACCAGCACAG GTTTTAGTGA CAAACGGGCC CGTTCCATGG ACATAGATGA CTTCATCAGA 
TTGCTACATG GATTCAACGC AGAAGGTATT CATTTTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIMT1 ( NM_014473.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055288.1 Homo sapiens DIMT1 rRNA methyltransferase and ribosome maturation factor (DIMT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06406
Clone ID Related Accession (Same CDS sequence)	NM_014473.3 , NM_014473.2 , NM_014473.4
Accession Version	NM_014473.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	probable dimethyladenosine transferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292723.1, AC016637.7 and BQ009563.1. On Nov 22, 2018 this sequence version replaced NM_014473.3. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803611.450841.1, SRR1660805.29519.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000199320.9/ ENSP00000199320.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGAAGG TCAAGTCGGG GGCCATCGGC CGCCGCCGCG GGCGGCAGGA GCAGCGCCGG 
GAGCTGAAGA GCGCTGGAGG ACTCATGTTC AACACGGGGA TTGGGCAGCA CATTTTGAAA 
AATCCTCTCA TTATTAACAG CATTATCGAT AAGGCTGCCT TAAGACCAAC TGATGTAGTG 
CTGGAAGTTG GACCTGGAAC TGGCAACATG ACTGTAAAGT TGTTAGAAAA GGCAAAAAAG 
GTTGTTGCTT GTGAACTTGA CCCAAGGCTA GTAGCTGAAC TTCACAAAAG AGTTCAGGGC 
ACGCCTGTGG CCAGCAAACT TCAAGTACTG GTGGGTGATG TGCTGAAAAC AGATTTGCCA 
TTCTTTGATA CTTGTGTGGC AAATTTGCCT TATCAGATCT CTTCACCTTT TGTCTTCAAG 
CTGTTGCTAC ATCGACCTTT TTTCAGGTGT GCTATACTTA TGTTTCAAAG AGAATTTGCC 
CTCCGACTGG TTGCAAAACC TGGAGATAAG TTATACTGCA GACTCTCAAT TAATACACAG 
CTGTTGGCAC GTGTGGACCA TCTAATGAAA GTGGGAAAGA ATAACTTCAG ACCACCGCCC 
AAGGTGGAAT CCAGTGTTGT AAGGATAGAA CCTAAGAATC CACCACCACC CATCAATTTT 
CAGGAATGGG ATGGTCTAGT AAGGATAACC TTTGTTAGGA AAAACAAGAC ACTCTCTGCT 
GCATTTAAAT CAAGTGCAGT GCAACAACTC TTGGAAAAAA ACTACAGAAT TCACTGTTCA 
GTCCATAATA TTATAATACC AGAAGATTTC AGCATAGCAG ATAAAATACA GCAAATCCTA 
ACCAGCACAG GTTTTAGTGA CAAACGGGCC CGTTCCATGG ACATAGATGA CTTCATCAGA 
TTGCTACATG GATTCAACGC AGAAGGTATT CATTTTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055288.1
CDS	134..1075
Translation	MPKVKSGAIGRRRGRQEQRRELKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCVANLPYQISSPFVFKLLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIIIPEDFSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS

Target ORF information:

RefSeq Version	NM_014473.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DIMT1 rRNA methyltransferase and ribosome maturation factor (DIMT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014473.4

ORF Insert Sequence:

ATGCCGAAGG TCAAGTCGGG GGCCATCGGC CGCCGCCGCG GGCGGCAGGA GCAGCGCCGG 
GAGCTGAAGA GCGCTGGAGG ACTCATGTTC AACACGGGGA TTGGGCAGCA CATTTTGAAA 
AATCCTCTCA TTATTAACAG CATTATCGAT AAGGCTGCCT TAAGACCAAC TGATGTAGTG 
CTGGAAGTTG GACCTGGAAC TGGCAACATG ACTGTAAAGT TGTTAGAAAA GGCAAAAAAG 
GTTGTTGCTT GTGAACTTGA CCCAAGGCTA GTAGCTGAAC TTCACAAAAG AGTTCAGGGC 
ACGCCTGTGG CCAGCAAACT TCAAGTACTG GTGGGTGATG TGCTGAAAAC AGATTTGCCA 
TTCTTTGATA CTTGTGTGGC AAATTTGCCT TATCAGATCT CTTCACCTTT TGTCTTCAAG 
CTGTTGCTAC ATCGACCTTT TTTCAGGTGT GCTATACTTA TGTTTCAAAG AGAATTTGCC 
CTCCGACTGG TTGCAAAACC TGGAGATAAG TTATACTGCA GACTCTCAAT TAATACACAG 
CTGTTGGCAC GTGTGGACCA TCTAATGAAA GTGGGAAAGA ATAACTTCAG ACCACCGCCC 
AAGGTGGAAT CCAGTGTTGT AAGGATAGAA CCTAAGAATC CACCACCACC CATCAATTTT 
CAGGAATGGG ATGGTCTAGT AAGGATAACC TTTGTTAGGA AAAACAAGAC ACTCTCTGCT 
GCATTTAAAT CAAGTGCAGT GCAACAACTC TTGGAAAAAA ACTACAGAAT TCACTGTTCA 
GTCCATAATA TTATAATACC AGAAGATTTC AGCATAGCAG ATAAAATACA GCAAATCCTA 
ACCAGCACAG GTTTTAGTGA CAAACGGGCC CGTTCCATGG ACATAGATGA CTTCATCAGA 
TTGCTACATG GATTCAACGC AGAAGGTATT CATTTTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIMT1 ( NM_001348077.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335006.1 Homo sapiens DIMT1 rRNA methyltransferase and ribosome maturation factor (DIMT1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu67177
Clone ID Related Accession (Same CDS sequence)	XM_011543323.2 , NM_001348077.1 , NM_001348077.2
Accession Version	NM_001348077.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	probable dimethyladenosine transferase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC016637.7. Transcript Variant: This variant (3) uses an alternate splice junction compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.94508.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTCAAA GAGAATTTGC CCTCCGACTG GTTGCAAAAC CTGGAGATAA GTTATACTGC 
AGACTCTCAA TTAATACACA GCTGTTGGCA CGTGTGGACC ATCTAATGAA AGTGGGAAAG 
AATAACTTCA GACCACCGCC CAAGGTGGAA TCCAGTGTTG TAAGGATAGA ACCTAAGAAT 
CCACCACCAC CCATCAATTT TCAGGAATGG GATGGTCTAG TAAGGATAAC CTTTGTTAGG 
AAAAACAAGA CACTCTCTGC TGCATTTAAA TCAAGTGCAG TGCAACAACT CTTGGAAAAA 
AACTACAGAA TTCACTGTTC AGTCCATAAT ATTATAATAC CAGAAGATTT CAGCATAGCA 
GATAAAATAC AGCAAATCCT AACCAGCACA GGTTTTAGTG ACAAACGGGC CCGTTCCATG 
GACATAGATG ACTTCATCAG ATTGCTACAT GGATTCAACG CAGAAGGTAT TCATTTTTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335006.1
CDS	730..1212
Translation	MFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIIIPEDFSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS

Target ORF information:

RefSeq Version	NM_001348077.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DIMT1 rRNA methyltransferase and ribosome maturation factor (DIMT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348077.1

ORF Insert Sequence:

ATGTTTCAAA GAGAATTTGC CCTCCGACTG GTTGCAAAAC CTGGAGATAA GTTATACTGC 
AGACTCTCAA TTAATACACA GCTGTTGGCA CGTGTGGACC ATCTAATGAA AGTGGGAAAG 
AATAACTTCA GACCACCGCC CAAGGTGGAA TCCAGTGTTG TAAGGATAGA ACCTAAGAAT 
CCACCACCAC CCATCAATTT TCAGGAATGG GATGGTCTAG TAAGGATAAC CTTTGTTAGG 
AAAAACAAGA CACTCTCTGC TGCATTTAAA TCAAGTGCAG TGCAACAACT CTTGGAAAAA 
AACTACAGAA TTCACTGTTC AGTCCATAAT ATTATAATAC CAGAAGATTT CAGCATAGCA 
GATAAAATAC AGCAAATCCT AACCAGCACA GGTTTTAGTG ACAAACGGGC CCGTTCCATG 
GACATAGATG ACTTCATCAG ATTGCTACAT GGATTCAACG CAGAAGGTAT TCATTTTTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIMT1 ( NM_001348077.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335006.1 Homo sapiens DIMT1 rRNA methyltransferase and ribosome maturation factor (DIMT1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu67177
Clone ID Related Accession (Same CDS sequence)	XM_011543323.2 , NM_001348077.1 , NM_001348077.2
Accession Version	NM_001348077.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	probable dimethyladenosine transferase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC016637.7. On May 31, 2019 this sequence version replaced NM_001348077.1. Transcript Variant: This variant (3) uses an alternate splice junction compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.94508.1, SRR3476690.652924.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTCAAA GAGAATTTGC CCTCCGACTG GTTGCAAAAC CTGGAGATAA GTTATACTGC 
AGACTCTCAA TTAATACACA GCTGTTGGCA CGTGTGGACC ATCTAATGAA AGTGGGAAAG 
AATAACTTCA GACCACCGCC CAAGGTGGAA TCCAGTGTTG TAAGGATAGA ACCTAAGAAT 
CCACCACCAC CCATCAATTT TCAGGAATGG GATGGTCTAG TAAGGATAAC CTTTGTTAGG 
AAAAACAAGA CACTCTCTGC TGCATTTAAA TCAAGTGCAG TGCAACAACT CTTGGAAAAA 
AACTACAGAA TTCACTGTTC AGTCCATAAT ATTATAATAC CAGAAGATTT CAGCATAGCA 
GATAAAATAC AGCAAATCCT AACCAGCACA GGTTTTAGTG ACAAACGGGC CCGTTCCATG 
GACATAGATG ACTTCATCAG ATTGCTACAT GGATTCAACG CAGAAGGTAT TCATTTTTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335006.1
CDS	675..1157
Translation	MFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIIIPEDFSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS

Target ORF information:

RefSeq Version	NM_001348077.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DIMT1 rRNA methyltransferase and ribosome maturation factor (DIMT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348077.2

ORF Insert Sequence:

ATGTTTCAAA GAGAATTTGC CCTCCGACTG GTTGCAAAAC CTGGAGATAA GTTATACTGC 
AGACTCTCAA TTAATACACA GCTGTTGGCA CGTGTGGACC ATCTAATGAA AGTGGGAAAG 
AATAACTTCA GACCACCGCC CAAGGTGGAA TCCAGTGTTG TAAGGATAGA ACCTAAGAAT 
CCACCACCAC CCATCAATTT TCAGGAATGG GATGGTCTAG TAAGGATAAC CTTTGTTAGG 
AAAAACAAGA CACTCTCTGC TGCATTTAAA TCAAGTGCAG TGCAACAACT CTTGGAAAAA 
AACTACAGAA TTCACTGTTC AGTCCATAAT ATTATAATAC CAGAAGATTT CAGCATAGCA 
GATAAAATAC AGCAAATCCT AACCAGCACA GGTTTTAGTG ACAAACGGGC CCGTTCCATG 
GACATAGATG ACTTCATCAG ATTGCTACAT GGATTCAACG CAGAAGGTAT TCATTTTTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIMT1 ( XM_011543323.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541625.1 Homo sapiens DIM1 dimethyladenosine transferase 1 homolog (DIMT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu67177
Clone ID Related Accession (Same CDS sequence)	XM_011543323.2 , NM_001348077.1 , NM_001348077.2
Accession Version	XM_011543323.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	probable dimethyladenosine transferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543323.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCAAA GAGAATTTGC CCTCCGACTG GTTGCAAAAC CTGGAGATAA GTTATACTGC 
AGACTCTCAA TTAATACACA GCTGTTGGCA CGTGTGGACC ATCTAATGAA AGTGGGAAAG 
AATAACTTCA GACCACCGCC CAAGGTGGAA TCCAGTGTTG TAAGGATAGA ACCTAAGAAT 
CCACCACCAC CCATCAATTT TCAGGAATGG GATGGTCTAG TAAGGATAAC CTTTGTTAGG 
AAAAACAAGA CACTCTCTGC TGCATTTAAA TCAAGTGCAG TGCAACAACT CTTGGAAAAA 
AACTACAGAA TTCACTGTTC AGTCCATAAT ATTATAATAC CAGAAGATTT CAGCATAGCA 
GATAAAATAC AGCAAATCCT AACCAGCACA GGTTTTAGTG ACAAACGGGC CCGTTCCATG 
GACATAGATG ACTTCATCAG ATTGCTACAT GGATTCAACG CAGAAGGTAT TCATTTTTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541625.1
CDS	709..1191
Translation	MFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIIIPEDFSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS

Target ORF information:

RefSeq Version	XM_011543323.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DIM1 dimethyladenosine transferase 1 homolog (DIMT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543323.2

ORF Insert Sequence:

ATGTTTCAAA GAGAATTTGC CCTCCGACTG GTTGCAAAAC CTGGAGATAA GTTATACTGC 
AGACTCTCAA TTAATACACA GCTGTTGGCA CGTGTGGACC ATCTAATGAA AGTGGGAAAG 
AATAACTTCA GACCACCGCC CAAGGTGGAA TCCAGTGTTG TAAGGATAGA ACCTAAGAAT 
CCACCACCAC CCATCAATTT TCAGGAATGG GATGGTCTAG TAAGGATAAC CTTTGTTAGG 
AAAAACAAGA CACTCTCTGC TGCATTTAAA TCAAGTGCAG TGCAACAACT CTTGGAAAAA 
AACTACAGAA TTCACTGTTC AGTCCATAAT ATTATAATAC CAGAAGATTT CAGCATAGCA 
GATAAAATAC AGCAAATCCT AACCAGCACA GGTTTTAGTG ACAAACGGGC CCGTTCCATG 
GACATAGATG ACTTCATCAG ATTGCTACAT GGATTCAACG CAGAAGGTAT TCATTTTTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIMT1 ( NM_001348076.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335005.1 Homo sapiens DIMT1 rRNA methyltransferase and ribosome maturation factor (DIMT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu102340
Clone ID Related Accession (Same CDS sequence)	NM_001348076.2 , NM_001348076.1
Accession Version	NM_001348076.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	probable dimethyladenosine transferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC398380.1, BC002841.2 and AC016637.7. On May 31, 2019 this sequence version replaced NM_001348076.1. Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC002841.2, SRR1163657.488682.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGAAGG TCAAGTCGGG GGCCATCGGC CGCCGCCGCG GGCGGCAGGA GCAGCGCCGG 
GAGCTGAAGA GCGCTGGAGG ACTCATGTTC AACACGGGGA TTGGGCAGCA CATTTTGAAA 
AATCCTCTCA TTATTAACAG CATTATCGAT AAGGCTGCCT TAAGACCAAC TGATGTAGTG 
CTGGAAGTTG GACCTGGAAC TGGCAACATG ACTGTAAAGT TGTTAGAAAA GGCAAAAAAG 
GTTGTTGCTT GTGAACTTGA CCCAAGGCTA GTAGCTGAAC TTCACAAAAG AGTTCAGGGC 
ACGCCTGTGG CCAGCAAACT TCAAGTACTG GTGGGTGATG TGCTGAAAAC AGATTTGCCA 
TTCTTTGATA CTTGTGTGGC AAATTTGCCT TATCAGATCT CTTCACCTTT TGTCTTCAAG 
CTGTTGCTAC ATCGACCTTT TTTCAGGTGT GCTATACTTA TGTTTCAAAG AGAATTTGCC 
CTCCGACTGG TTGCAAAACC TGGAGATAAG TTATACTGCA GACTCTCAAT TAATACACAG 
CTGTTGGCAC GTGTGGACCA TCTAATGAAA GTGGGAAAGA ATAACTTCAG ACCACCGCCC 
AAGGTGGAAT CCAGTGTTGT AAGGATAGAA CCTAAGAATC CACCACCACC CATCAATTTT 
CAGGAATGGG ATGGTCTAGT AAGGATAACC TTTGTTAGGA AAAACAAGAC ACTCTCTGCT 
GCATTTAAAT CAAGTGCAGT GCAACAACTC TTGGAAAAAA ACTACAGAAT TCACTGTTCA 
GTCCATAATA TTGTCAGTGC TGCTGTGTAT CCTGTGAAAC AGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335005.1
CDS	134..961
Translation	MPKVKSGAIGRRRGRQEQRRELKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCVANLPYQISSPFVFKLLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIVSAAVYPVKQI

Target ORF information:

RefSeq Version	NM_001348076.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DIMT1 rRNA methyltransferase and ribosome maturation factor (DIMT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348076.2

ORF Insert Sequence:

ATGCCGAAGG TCAAGTCGGG GGCCATCGGC CGCCGCCGCG GGCGGCAGGA GCAGCGCCGG 
GAGCTGAAGA GCGCTGGAGG ACTCATGTTC AACACGGGGA TTGGGCAGCA CATTTTGAAA 
AATCCTCTCA TTATTAACAG CATTATCGAT AAGGCTGCCT TAAGACCAAC TGATGTAGTG 
CTGGAAGTTG GACCTGGAAC TGGCAACATG ACTGTAAAGT TGTTAGAAAA GGCAAAAAAG 
GTTGTTGCTT GTGAACTTGA CCCAAGGCTA GTAGCTGAAC TTCACAAAAG AGTTCAGGGC 
ACGCCTGTGG CCAGCAAACT TCAAGTACTG GTGGGTGATG TGCTGAAAAC AGATTTGCCA 
TTCTTTGATA CTTGTGTGGC AAATTTGCCT TATCAGATCT CTTCACCTTT TGTCTTCAAG 
CTGTTGCTAC ATCGACCTTT TTTCAGGTGT GCTATACTTA TGTTTCAAAG AGAATTTGCC 
CTCCGACTGG TTGCAAAACC TGGAGATAAG TTATACTGCA GACTCTCAAT TAATACACAG 
CTGTTGGCAC GTGTGGACCA TCTAATGAAA GTGGGAAAGA ATAACTTCAG ACCACCGCCC 
AAGGTGGAAT CCAGTGTTGT AAGGATAGAA CCTAAGAATC CACCACCACC CATCAATTTT 
CAGGAATGGG ATGGTCTAGT AAGGATAACC TTTGTTAGGA AAAACAAGAC ACTCTCTGCT 
GCATTTAAAT CAAGTGCAGT GCAACAACTC TTGGAAAAAA ACTACAGAAT TCACTGTTCA 
GTCCATAATA TTGTCAGTGC TGCTGTGTAT CCTGTGAAAC AGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIMT1 ( NM_001348076.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335005.1 Homo sapiens DIMT1 rRNA methyltransferase and ribosome maturation factor (DIMT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu102340
Clone ID Related Accession (Same CDS sequence)	NM_001348076.2 , NM_001348076.1
Accession Version	NM_001348076.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	probable dimethyladenosine transferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC398380.1, BC002841.2 and AC016637.7. Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC002841.2, SRR1163657.488682.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGAAGG TCAAGTCGGG GGCCATCGGC CGCCGCCGCG GGCGGCAGGA GCAGCGCCGG 
GAGCTGAAGA GCGCTGGAGG ACTCATGTTC AACACGGGGA TTGGGCAGCA CATTTTGAAA 
AATCCTCTCA TTATTAACAG CATTATCGAT AAGGCTGCCT TAAGACCAAC TGATGTAGTG 
CTGGAAGTTG GACCTGGAAC TGGCAACATG ACTGTAAAGT TGTTAGAAAA GGCAAAAAAG 
GTTGTTGCTT GTGAACTTGA CCCAAGGCTA GTAGCTGAAC TTCACAAAAG AGTTCAGGGC 
ACGCCTGTGG CCAGCAAACT TCAAGTACTG GTGGGTGATG TGCTGAAAAC AGATTTGCCA 
TTCTTTGATA CTTGTGTGGC AAATTTGCCT TATCAGATCT CTTCACCTTT TGTCTTCAAG 
CTGTTGCTAC ATCGACCTTT TTTCAGGTGT GCTATACTTA TGTTTCAAAG AGAATTTGCC 
CTCCGACTGG TTGCAAAACC TGGAGATAAG TTATACTGCA GACTCTCAAT TAATACACAG 
CTGTTGGCAC GTGTGGACCA TCTAATGAAA GTGGGAAAGA ATAACTTCAG ACCACCGCCC 
AAGGTGGAAT CCAGTGTTGT AAGGATAGAA CCTAAGAATC CACCACCACC CATCAATTTT 
CAGGAATGGG ATGGTCTAGT AAGGATAACC TTTGTTAGGA AAAACAAGAC ACTCTCTGCT 
GCATTTAAAT CAAGTGCAGT GCAACAACTC TTGGAAAAAA ACTACAGAAT TCACTGTTCA 
GTCCATAATA TTGTCAGTGC TGCTGTGTAT CCTGTGAAAC AGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335005.1
CDS	189..1016
Translation	MPKVKSGAIGRRRGRQEQRRELKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCVANLPYQISSPFVFKLLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIVSAAVYPVKQI

Target ORF information:

RefSeq Version	NM_001348076.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DIMT1 rRNA methyltransferase and ribosome maturation factor (DIMT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348076.1

ORF Insert Sequence:

ATGCCGAAGG TCAAGTCGGG GGCCATCGGC CGCCGCCGCG GGCGGCAGGA GCAGCGCCGG 
GAGCTGAAGA GCGCTGGAGG ACTCATGTTC AACACGGGGA TTGGGCAGCA CATTTTGAAA 
AATCCTCTCA TTATTAACAG CATTATCGAT AAGGCTGCCT TAAGACCAAC TGATGTAGTG 
CTGGAAGTTG GACCTGGAAC TGGCAACATG ACTGTAAAGT TGTTAGAAAA GGCAAAAAAG 
GTTGTTGCTT GTGAACTTGA CCCAAGGCTA GTAGCTGAAC TTCACAAAAG AGTTCAGGGC 
ACGCCTGTGG CCAGCAAACT TCAAGTACTG GTGGGTGATG TGCTGAAAAC AGATTTGCCA 
TTCTTTGATA CTTGTGTGGC AAATTTGCCT TATCAGATCT CTTCACCTTT TGTCTTCAAG 
CTGTTGCTAC ATCGACCTTT TTTCAGGTGT GCTATACTTA TGTTTCAAAG AGAATTTGCC 
CTCCGACTGG TTGCAAAACC TGGAGATAAG TTATACTGCA GACTCTCAAT TAATACACAG 
CTGTTGGCAC GTGTGGACCA TCTAATGAAA GTGGGAAAGA ATAACTTCAG ACCACCGCCC 
AAGGTGGAAT CCAGTGTTGT AAGGATAGAA CCTAAGAATC CACCACCACC CATCAATTTT 
CAGGAATGGG ATGGTCTAGT AAGGATAACC TTTGTTAGGA AAAACAAGAC ACTCTCTGCT 
GCATTTAAAT CAAGTGCAGT GCAACAACTC TTGGAAAAAA ACTACAGAAT TCACTGTTCA 
GTCCATAATA TTGTCAGTGC TGCTGTGTAT CCTGTGAAAC AGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DIMT1 overexpression correlates with progression and prognosis in gastric carcinoma.
Human pathology7035-42(2017 Dec)
Liu G,Peng X,Cai Y,Cheng A,Zha L,Wang Z

Hypoxia-inducible microRNA-210 regulates the DIMT1-IRF4 oncogenic axis in multiple myeloma.
Cancer science108(4)641-652(2017 Apr)
Ikeda S,Kitadate A,Abe F,Saitoh H,Michishita Y,Hatano Y,Kawabata Y,Kitabayashi A,Teshima K,Kume M,Takahashi N,Tagawa H

The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis.
Molecular biology of the cell26(11)2080-95(2015 Jun)
Zorbas C,Nicolas E,Wacheul L,Huvelle E,Heurgu?-Hamard V,Lafontaine DL

Epigenetic silencing of miR-210 increases the proliferation of gastric epithelium during chronic Helicobacter pylori infection.
Nature communications54497(2014 Sep)
Kiga K,Mimuro H,Suzuki M,Shinozaki-Ushiku A,Kobayashi T,Sanada T,Kim M,Ogawa M,Iwasaki YW,Kayo H,Fukuda-Yuzawa Y,Yashiro M,Fukayama M,Fukao T,Sasakawa C

Characterization of 16 novel human genes showing high similarity to yeast sequences.
Yeast (Chichester, England)18(1)69-80(2001 Jan)
Stanchi F,Bertocco E,Toppo S,Dioguardi R,Simionati B,Cannata N,Zimbello R,Lanfranchi G,Valle G

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DIMT1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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