HCAR1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HCAR1
Entrez Gene ID	27198
Full Name	hydroxycarboxylic acid receptor 1
Synonyms	FKSG80,GPR104,GPR81,HCA1,LACR1,TA-GPCR,TAGPCR
General protein information	Preferred Names hydroxycarboxylic acid receptor 1 Names hydroxycarboxylic acid receptor 1 G protein-coupled receptor 104 G-protein coupled receptor 81 T-cell activation G protein-coupled receptor hydroxy-carboxylic acid receptor 1 lactate receptor 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.31
Summary	G protein-coupled receptors (GPCRs, or GPRs), such as GPR81, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.[supplied by OMIM, Feb 2005].
Disorder MIM:	606923

mRNA and Protein(s)

mRNA	Protein	Name
NM_032554.3	NP_115943.1	hydroxycarboxylic acid receptor 1
NM_032554.4	NP_115943.1	hydroxycarboxylic acid receptor 1

Pathways from BioSystems

Homologies

	HCAR1	NP_001138726.1
Bos taurus (cattle)	HCAR1	NP_001138706.1
Pan troglodytes (chimpanzee)	HCAR1	NP_001233564.1
Canis lupus familiaris (dog)	HCAR1	NP_001138703.1
Rattus norvegicus (Norway rat)	Gpr81	NP_001138806.1
Homo sapiens (human)	HCAR1	NP_115943.1
Mus musculus (house mouse)	Gpr81	NP_780729.2

Gene Ontology
Bibliography

Related articles in PubMed

Tumor cell-derived lactate induces TAZ-dependent upregulation of PD-L1 through GPR81 in human lung cancer cells.
Feng J, Yang H, Zhang Y, Wei H, Zhu Z, Zhu B, Yang M, Cao W, Wang L, Wu Z
Oncogene36(42)5829-5839(2017 Oct)

The lactate receptor (HCAR1/GPR81) contributes to doxorubicin chemoresistance via ABCB1 transporter up-regulation in human cervical cancer HeLa cells.
Wagner W, Kania KD, Blauz A, Ciszewski WM
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society68(4)555-564(2017 Aug)

Stimulation of lactate receptor (HCAR1) affects cellular DNA repair capacity.
Wagner W, Kania KD, Ciszewski WM
DNA repair5249-58(2017 04)

G-protein-coupled receptor 81 promotes a malignant phenotype in breast cancer through angiogenic factor secretion.
Lee YJ, Shin KJ, Park SA, Park KS, Park S, Heo K, Seo YK, Noh DY, Ryu SH, Suh PG
Oncotarget7(43)70898-70911(2016 Oct)

L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation.
Wagner W, Ciszewski WM, Kania KD
Cell communication and signaling : CCS1336(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Activation of GPR81 decreases intracellular cAMP levels and inhibits PKA activity, leading to activation of TAZ and upregulation of PD-L1. This study reveals a critical role for lactate in the immune checkpoint pathway and an unexpected function of TAZ in tumor evasion of the T-cell-mediated immune response.
Title: Tumor cell-derived lactate induces TAZ-dependent upregulation of PD-L1 through GPR81 in human lung cancer cells.

Lactate receptor/HCAR1 expression in cervical carcinoma cells may contribute to the modulation of cellular DNA repair mechanisms.
Title: Stimulation of lactate receptor (HCAR1) affects cellular DNA repair capacity.

HCA1/3 are necessary for breast cancer cells to balance lipid/fatty acid metabolism.
Title: Hydroxycarboxylic acid receptors are essential for breast cancer cells to control their lipid/fatty acid metabolism.

HCAR1 and monocarboxylate transporters activity is required for the L- and D-lactate-mediated enhancement of DNA repair and of HeLa cell survival.
Title: L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation.

Data support that GPR81 is important for cancer cell regulation of lactate transport mechanisms.
Title: Cell surface lactate receptor GPR81 is crucial for cancer cell survival.

The following HCAR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCAR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30348	NM_032554.4 Latest version!	Homo sapiens hydroxycarboxylic acid receptor 1 (HCAR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu30348	NM_032554.3	Homo sapiens hydroxycarboxylic acid receptor 1 (HCAR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HCAR1 ( NM_032554.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_115943.1 Homo sapiens hydroxycarboxylic acid receptor 1 (HCAR1), mRNA.

Price & Availability↑

CloneID	OHu30348
Clone ID Related Accession (Same CDS sequence)	NM_032554.3 , NM_032554.4
Accession Version	NM_032554.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	hydroxycarboxylic acid receptor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC026333.28 and AF345568.1. On Feb 11, 2006 this sequence version replaced NM_032554.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGTACAACG GGTCGTGCTG CCGCATCGAG GGGGACACCA TCTCCCAGGT GATGCCGCCG 
CTGCTCATTG TGGCCTTTGT GCTGGGCGCA CTAGGCAATG GGGTCGCCCT GTGTGGTTTC 
TGCTTCCACA TGAAGACCTG GAAGCCCAGC ACTGTTTACC TTTTCAATTT GGCCGTGGCT 
GATTTCCTCC TTATGATCTG CCTGCCTTTT CGGACAGACT ATTACCTCAG ACGTAGACAC 
TGGGCTTTTG GGGACATTCC CTGCCGAGTG GGGCTCTTCA CGTTGGCCAT GAACAGGGCC 
GGGAGCATCG TGTTCCTTAC GGTGGTGGCT GCGGACAGGT ATTTCAAAGT GGTCCACCCC 
CACCACGCGG TGAACACTAT CTCCACCCGG GTGGCGGCTG GCATCGTCTG CACCCTGTGG 
GCCCTGGTCA TCCTGGGAAC AGTGTATCTT TTGCTGGAGA ACCATCTCTG CGTGCAAGAG 
ACGGCCGTCT CCTGTGAGAG CTTCATCATG GAGTCGGCCA ATGGCTGGCA TGACATCATG 
TTCCAGCTGG AGTTCTTTAT GCCCCTCGGC ATCATCTTAT TTTGCTCCTT CAAGATTGTT 
TGGAGCCTGA GGCGGAGGCA GCAGCTGGCC AGACAGGCTC GGATGAAGAA GGCGACCCGG 
TTCATCATGG TGGTGGCAAT TGTGTTCATC ACATGCTACC TGCCCAGCGT GTCTGCTAGA 
CTCTATTTCC TCTGGACGGT GCCCTCGAGT GCCTGCGATC CCTCTGTCCA TGGGGCCCTG 
CACATAACCC TCAGCTTCAC CTACATGAAC AGCATGCTGG ATCCCCTGGT GTATTATTTT 
TCAAGCCCCT CCTTTCCCAA ATTCTACAAC AAGCTCAAAA TCTGCAGTCT GAAACCCAAG 
CAGCCAGGAC ACTCAAAAAC ACAAAGGCCG GAAGAGATGC CAATTTCGAA CCTCGGTCGC 
AGGAGTTGCA TCAGTGTGGC AAATAGTTTC CAAAGCCAGT CTGATGGGCA ATGGGATCCC 
CACATTGTTG AGTGGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115943.1
CDS	244..1284
Translation	MYNGSCCRIEGDTISQVMPPLLIVAFVLGALGNGVALCGFCFHMKTWKPSTVYLFNLAVADFLLMICLPFRTDYYLRRRHWAFGDIPCRVGLFTLAMNRAGSIVFLTVVAADRYFKVVHPHHAVNTISTRVAAGIVCTLWALVILGTVYLLLENHLCVQETAVSCESFIMESANGWHDIMFQLEFFMPLGIILFCSFKIVWSLRRRQQLARQARMKKATRFIMVVAIVFITCYLPSVSARLYFLWTVPSSACDPSVHGALHITLSFTYMNSMLDPLVYYFSSPSFPKFYNKLKICSLKPKQPGHSKTQRPEEMPISNLGRRSCISVANSFQSQSDGQWDPHIVEWH

Target ORF information:

RefSeq Version	NM_032554.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hydroxycarboxylic acid receptor 1 (HCAR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032554.3

ORF Insert Sequence:

ATGTACAACG GGTCGTGCTG CCGCATCGAG GGGGACACCA TCTCCCAGGT GATGCCGCCG 
CTGCTCATTG TGGCCTTTGT GCTGGGCGCA CTAGGCAATG GGGTCGCCCT GTGTGGTTTC 
TGCTTCCACA TGAAGACCTG GAAGCCCAGC ACTGTTTACC TTTTCAATTT GGCCGTGGCT 
GATTTCCTCC TTATGATCTG CCTGCCTTTT CGGACAGACT ATTACCTCAG ACGTAGACAC 
TGGGCTTTTG GGGACATTCC CTGCCGAGTG GGGCTCTTCA CGTTGGCCAT GAACAGGGCC 
GGGAGCATCG TGTTCCTTAC GGTGGTGGCT GCGGACAGGT ATTTCAAAGT GGTCCACCCC 
CACCACGCGG TGAACACTAT CTCCACCCGG GTGGCGGCTG GCATCGTCTG CACCCTGTGG 
GCCCTGGTCA TCCTGGGAAC AGTGTATCTT TTGCTGGAGA ACCATCTCTG CGTGCAAGAG 
ACGGCCGTCT CCTGTGAGAG CTTCATCATG GAGTCGGCCA ATGGCTGGCA TGACATCATG 
TTCCAGCTGG AGTTCTTTAT GCCCCTCGGC ATCATCTTAT TTTGCTCCTT CAAGATTGTT 
TGGAGCCTGA GGCGGAGGCA GCAGCTGGCC AGACAGGCTC GGATGAAGAA GGCGACCCGG 
TTCATCATGG TGGTGGCAAT TGTGTTCATC ACATGCTACC TGCCCAGCGT GTCTGCTAGA 
CTCTATTTCC TCTGGACGGT GCCCTCGAGT GCCTGCGATC CCTCTGTCCA TGGGGCCCTG 
CACATAACCC TCAGCTTCAC CTACATGAAC AGCATGCTGG ATCCCCTGGT GTATTATTTT 
TCAAGCCCCT CCTTTCCCAA ATTCTACAAC AAGCTCAAAA TCTGCAGTCT GAAACCCAAG 
CAGCCAGGAC ACTCAAAAAC ACAAAGGCCG GAAGAGATGC CAATTTCGAA CCTCGGTCGC 
AGGAGTTGCA TCAGTGTGGC AAATAGTTTC CAAAGCCAGT CTGATGGGCA ATGGGATCCC 
CACATTGTTG AGTGGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCAR1 ( NM_032554.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_115943.1 Homo sapiens hydroxycarboxylic acid receptor 1 (HCAR1), mRNA.

Price & Availability↑

CloneID	OHu30348
Clone ID Related Accession (Same CDS sequence)	NM_032554.3 , NM_032554.4
Accession Version	NM_032554.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	hydroxycarboxylic acid receptor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC026333.28 and AF345568.1. On May 17, 2019 this sequence version replaced NM_032554.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: SRR1803615.5340.1, AK289557.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000432564.3/ ENSP00000389255.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGTACAACG GGTCGTGCTG CCGCATCGAG GGGGACACCA TCTCCCAGGT GATGCCGCCG 
CTGCTCATTG TGGCCTTTGT GCTGGGCGCA CTAGGCAATG GGGTCGCCCT GTGTGGTTTC 
TGCTTCCACA TGAAGACCTG GAAGCCCAGC ACTGTTTACC TTTTCAATTT GGCCGTGGCT 
GATTTCCTCC TTATGATCTG CCTGCCTTTT CGGACAGACT ATTACCTCAG ACGTAGACAC 
TGGGCTTTTG GGGACATTCC CTGCCGAGTG GGGCTCTTCA CGTTGGCCAT GAACAGGGCC 
GGGAGCATCG TGTTCCTTAC GGTGGTGGCT GCGGACAGGT ATTTCAAAGT GGTCCACCCC 
CACCACGCGG TGAACACTAT CTCCACCCGG GTGGCGGCTG GCATCGTCTG CACCCTGTGG 
GCCCTGGTCA TCCTGGGAAC AGTGTATCTT TTGCTGGAGA ACCATCTCTG CGTGCAAGAG 
ACGGCCGTCT CCTGTGAGAG CTTCATCATG GAGTCGGCCA ATGGCTGGCA TGACATCATG 
TTCCAGCTGG AGTTCTTTAT GCCCCTCGGC ATCATCTTAT TTTGCTCCTT CAAGATTGTT 
TGGAGCCTGA GGCGGAGGCA GCAGCTGGCC AGACAGGCTC GGATGAAGAA GGCGACCCGG 
TTCATCATGG TGGTGGCAAT TGTGTTCATC ACATGCTACC TGCCCAGCGT GTCTGCTAGA 
CTCTATTTCC TCTGGACGGT GCCCTCGAGT GCCTGCGATC CCTCTGTCCA TGGGGCCCTG 
CACATAACCC TCAGCTTCAC CTACATGAAC AGCATGCTGG ATCCCCTGGT GTATTATTTT 
TCAAGCCCCT CCTTTCCCAA ATTCTACAAC AAGCTCAAAA TCTGCAGTCT GAAACCCAAG 
CAGCCAGGAC ACTCAAAAAC ACAAAGGCCG GAAGAGATGC CAATTTCGAA CCTCGGTCGC 
AGGAGTTGCA TCAGTGTGGC AAATAGTTTC CAAAGCCAGT CTGATGGGCA ATGGGATCCC 
CACATTGTTG AGTGGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115943.1
CDS	506..1546
Translation	MYNGSCCRIEGDTISQVMPPLLIVAFVLGALGNGVALCGFCFHMKTWKPSTVYLFNLAVADFLLMICLPFRTDYYLRRRHWAFGDIPCRVGLFTLAMNRAGSIVFLTVVAADRYFKVVHPHHAVNTISTRVAAGIVCTLWALVILGTVYLLLENHLCVQETAVSCESFIMESANGWHDIMFQLEFFMPLGIILFCSFKIVWSLRRRQQLARQARMKKATRFIMVVAIVFITCYLPSVSARLYFLWTVPSSACDPSVHGALHITLSFTYMNSMLDPLVYYFSSPSFPKFYNKLKICSLKPKQPGHSKTQRPEEMPISNLGRRSCISVANSFQSQSDGQWDPHIVEWH

Target ORF information:

RefSeq Version	NM_032554.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hydroxycarboxylic acid receptor 1 (HCAR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032554.4

ORF Insert Sequence:

ATGTACAACG GGTCGTGCTG CCGCATCGAG GGGGACACCA TCTCCCAGGT GATGCCGCCG 
CTGCTCATTG TGGCCTTTGT GCTGGGCGCA CTAGGCAATG GGGTCGCCCT GTGTGGTTTC 
TGCTTCCACA TGAAGACCTG GAAGCCCAGC ACTGTTTACC TTTTCAATTT GGCCGTGGCT 
GATTTCCTCC TTATGATCTG CCTGCCTTTT CGGACAGACT ATTACCTCAG ACGTAGACAC 
TGGGCTTTTG GGGACATTCC CTGCCGAGTG GGGCTCTTCA CGTTGGCCAT GAACAGGGCC 
GGGAGCATCG TGTTCCTTAC GGTGGTGGCT GCGGACAGGT ATTTCAAAGT GGTCCACCCC 
CACCACGCGG TGAACACTAT CTCCACCCGG GTGGCGGCTG GCATCGTCTG CACCCTGTGG 
GCCCTGGTCA TCCTGGGAAC AGTGTATCTT TTGCTGGAGA ACCATCTCTG CGTGCAAGAG 
ACGGCCGTCT CCTGTGAGAG CTTCATCATG GAGTCGGCCA ATGGCTGGCA TGACATCATG 
TTCCAGCTGG AGTTCTTTAT GCCCCTCGGC ATCATCTTAT TTTGCTCCTT CAAGATTGTT 
TGGAGCCTGA GGCGGAGGCA GCAGCTGGCC AGACAGGCTC GGATGAAGAA GGCGACCCGG 
TTCATCATGG TGGTGGCAAT TGTGTTCATC ACATGCTACC TGCCCAGCGT GTCTGCTAGA 
CTCTATTTCC TCTGGACGGT GCCCTCGAGT GCCTGCGATC CCTCTGTCCA TGGGGCCCTG 
CACATAACCC TCAGCTTCAC CTACATGAAC AGCATGCTGG ATCCCCTGGT GTATTATTTT 
TCAAGCCCCT CCTTTCCCAA ATTCTACAAC AAGCTCAAAA TCTGCAGTCT GAAACCCAAG 
CAGCCAGGAC ACTCAAAAAC ACAAAGGCCG GAAGAGATGC CAATTTCGAA CCTCGGTCGC 
AGGAGTTGCA TCAGTGTGGC AAATAGTTTC CAAAGCCAGT CTGATGGGCA ATGGGATCCC 
CACATTGTTG AGTGGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tumor cell-derived lactate induces TAZ-dependent upregulation of PD-L1 through GPR81 in human lung cancer cells.
Oncogene36(42)5829-5839(2017 Oct)
Feng J,Yang H,Zhang Y,Wei H,Zhu Z,Zhu B,Yang M,Cao W,Wang L,Wu Z

The lactate receptor (HCAR1/GPR81) contributes to doxorubicin chemoresistance via ABCB1 transporter up-regulation in human cervical cancer HeLa cells.
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society68(4)555-564(2017 Aug)
Wagner W,Kania KD,Blauz A,Ciszewski WM

Stimulation of lactate receptor (HCAR1) affects cellular DNA repair capacity.
DNA repair5249-58(2017 04)
Wagner W,Kania KD,Ciszewski WM

G-protein-coupled receptor 81 promotes a malignant phenotype in breast cancer through angiogenic factor secretion.
Oncotarget7(43)70898-70911(2016 Oct)
Lee YJ,Shin KJ,Park SA,Park KS,Park S,Heo K,Seo YK,Noh DY,Ryu SH,Suh PG

L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation.
Cell communication and signaling : CCS1336(2015 Jul)
Wagner W,Ciszewski WM,Kania KD

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HCAR1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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