SIGLEC8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SIGLEC8
Entrez Gene ID	27181
Full Name	sialic acid binding Ig like lectin 8
Synonyms	SAF2,SIGLEC-8,SIGLEC8L
General protein information	Preferred Names sialic acid binding Ig like lectin 8 Names sialic acid-binding Ig-like lectin 8 CDw329 SAF-2 sialoadhesin family member 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.41
Summary	Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010].
Disorder MIM:	605639

mRNA and Protein(s)

mRNA	Protein	Name
NM_014442.3	NP_055257.2	sialic acid-binding Ig-like lectin 8 isoform 1 precursor
NM_001363548.1	NP_001350477.1	sialic acid-binding Ig-like lectin 8 isoform 2 precursor
NM_014442.2	NP_055257.2	sialic acid-binding Ig-like lectin 8 precursor
XM_011526734.2	XP_011525036.1	sialic acid-binding Ig-like lectin 8 isoform X1
XM_011526735.2	XP_011525037.1	sialic acid-binding Ig-like lectin 8 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SIGLEC8	NP_055257.2
Mus musculus (house mouse)	Siglec5	NP_663556.1
Rattus norvegicus (Norway rat)	Siglec5	NP_001099719.2
Canis lupus familiaris (dog)	SIGLEC12	XP_005616307.1
Bos taurus (cattle)	LOC618463	XP_002695268.2

Gene Ontology
Bibliography

Related articles in PubMed

Sialic acid-binding immunoglobulin-like lectin 8 (Siglec-8) is an activating receptor mediating ?
Carroll DJ, O'Sullivan JA, Nix DB, Cao Y, Tiemeyer M, Bochner BS
The Journal of allergy and clinical immunology(2017 Sep)

Leveraging Siglec-8 endocytic mechanisms to kill human eosinophils and malignant mast cells.
O'Sullivan JA, Carroll DJ, Cao Y, Salicru AN, Bochner BS
The Journal of allergy and clinical immunology(2017 Jul)

Olig2 is expressed late in human eosinophil development and controls Siglec-8 expression.
Hwang SM, Uhm TG, Lee SK, Kong SK, Jung KH, Binas B, Chai YG, Park SW, Chung IY
Journal of leukocyte biology100(4)711-723(2016 10)

Decreased expression of Siglec-8 associates with poor prognosis in patients with gastric cancer after surgical resection.
Cao Y, Liu H, Zhang H, Lin C, Li R, Zhang W, Shen Z, Xu J
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(8)10883-91(2016 Aug)

Role of MiR-215 in Hirschsprung's Disease Pathogenesis by Targeting SIGLEC-8.
Lei H, Li H, Xie H, Du C, Xia Y, Tang W
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology40(6)1646-1655(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In differentiating ,eosinophils, SIGLEC-8 messenger RNA and protein were markedly down-regulated in parallel with OLIG2 by an OLIG2 small interfering RNA or a short hairpin RNA. An E-box in the first intron was found to stimulate SIGLEC-8 gene transcription and to bind OLIG2.
Title: Olig2 is expressed late in human eosinophil development and controls Siglec-8 expression.

Our study suggested that intratumoral Siglec-8 expression was an independent prognostic factor for overall survival of patients with gastric cancer
Title: Decreased expression of Siglec-8 associates with poor prognosis in patients with gastric cancer after surgical resection.

Ligands for Siglec-8 and Siglec-9 may regulate the function of eosinophils, mast cells, neutrophils, and other cells in sinus mucosa.
Title: Regulation of airway inflammation by Siglec-8 and Siglec-9 sialoglycan ligand expression.

miR-215 acts in concert with the host gene IARS2 to affect neuron migration and proliferation through the target gene SIGLEC-8.
Title: Role of MiR-215 in Hirschsprung's Disease Pathogenesis by Targeting SIGLEC-8.

Inflammation results in up-regulation of immuneinhibitory Siglec-8 and Siglec-9 sialoglycan ligands on human airways.
Title: Expression of ligands for Siglec-8 and Siglec-9 in human airways and airway cells.

The following SIGLEC8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIGLEC8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06385	NM_014442.3 Latest version!	Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu67134	NM_001363548.1 Latest version!	Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu67133	XM_011526734.2 Latest version!	Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu67134	XM_011526735.2 Latest version!	Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu06385	NM_014442.2	Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIGLEC8 ( NM_014442.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055257.2 Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06385
Clone ID Related Accession (Same CDS sequence)	NM_014442.3 , NM_014442.2
Accession Version	NM_014442.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	sialic acid-binding Ig-like lectin 8 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA388103.1, BC053319.1 and AF195092.1. On Nov 22, 2018 this sequence version replaced NM_014442.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053319.1, SRR1803611.271576.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159764 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000321424.7/ ENSP00000321077.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCTGC TGCTGCTGCT GCTGCCCCTG CTCTGGGGGA CAAAGGGGAT GGAGGGAGAC 
AGACAATATG GGGATGGTTA CTTGCTGCAA GTGCAGGAGC TGGTGACGGT GCAGGAGGGC 
CTGTGTGTCC ATGTGCCCTG CTCCTTCTCC TACCCCCAGG ATGGCTGGAC TGACTCTGAC 
CCAGTTCATG GCTACTGGTT CCGGGCAGGA GACAGACCAT ACCAAGACGC TCCAGTGGCC 
ACAAACAACC CAGACAGAGA AGTGCAGGCA GAGACCCAGG GCCGATTCCA ACTCCTTGGG 
GACATTTGGA GCAACGACTG CTCCCTGAGC ATCAGAGACG CCAGGAAGAG GGATAAGGGG 
TCATATTTCT TTCGGCTAGA GAGAGGAAGC ATGAAATGGA GTTACAAATC ACAGTTGAAT 
TACAAAACTA AGCAGCTGTC TGTGTTTGTG ACAGCCCTGA CCCATAGGCC TGACATCCTC 
ATCCTAGGGA CCCTAGAGTC TGGCCACTCC AGGAACCTGA CCTGCTCTGT GCCCTGGGCC 
TGTAAGCAGG GGACACCCCC CATGATCTCC TGGATTGGGG CCTCCGTGTC CTCCCCGGGC 
CCCACTACTG CCCGCTCCTC AGTGCTCACC CTTACCCCAA AGCCCCAGGA CCACGGCACC 
AGCCTCACCT GTCAGGTGAC CTTGCCTGGG ACAGGTGTGA CCACGACCAG TACCGTCCGC 
CTCGATGTGT CCTACCCTCC TTGGAACTTG ACCATGACTG TCTTCCAAGG AGATGCCACA 
GCATCCACAG CCCTGGGAAA TGGCTCATCT CTTTCAGTCC TTGAGGGCCA GTCTCTGCGC 
CTGGTCTGTG CTGTCAACAG CAATCCCCCT GCCAGGCTGA GCTGGACCCG GGGGAGCCTG 
ACCCTGTGCC CCTCACGGTC CTCAAACCCT GGGCTGCTGG AGCTGCCTCG AGTGCACGTG 
AGGGATGAAG GGGAATTCAC CTGCCGAGCT CAGAACGCTC AGGGCTCCCA GCACATTTCC 
CTGAGCCTCT CCCTGCAGAA TGAGGGCACA GGCACCTCAA GACCTGTATC ACAAGTGACA 
CTGGCAGCAG TCGGGGGAGC TGGAGCCACA GCCCTGGCCT TCCTGTCCTT CTGCATCATC 
TTCATCATAG TGAGGTCCTG CAGGAAGAAA TCGGCAAGGC CAGCAGCGGG CGTGGGGGAT 
ACAGGCATGG AAGATGCAAA GGCCATCAGG GGCTCGGCCT CTCAGGGACC CCTGACTGAA 
TCCTGGAAAG ATGGCAACCC CCTGAAGAAG CCTCCCCCAG CTGTTGCCCC CTCGTCAGGG 
GAGGAAGGAG AGCTCCATTA TGCAACCCTC AGCTTCCATA AAGTGAAGCC TCAGGACCCG 
CAGGGACAGG AGGCCACTGA CAGTGAATAC TCGGAGATCA AGATCCACAA GCGAGAAACT 
GCAGAGACTC AGGCCTGTTT GAGGAATCAC AACCCCTCCA GCAAAGAAGT CAGAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055257.2
CDS	68..1567
Translation	MLLLLLLLPLLWGTKGMEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTALTHRPDILILGTLESGHSRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLTLTPKPQDHGTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAAVGGAGATALAFLSFCIIFIIVRSCRKKSARPAAGVGDTGMEDAKAIRGSASQGPLTESWKDGNPLKKPPPAVAPSSGEEGELHYATLSFHKVKPQDPQGQEATDSEYSEIKIHKRETAETQACLRNHNPSSKEVRG

Target ORF information:

RefSeq Version	NM_014442.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014442.3

ORF Insert Sequence:

ATGCTGCTGC TGCTGCTGCT GCTGCCCCTG CTCTGGGGGA CAAAGGGGAT GGAGGGAGAC 
AGACAATATG GGGATGGTTA CTTGCTGCAA GTGCAGGAGC TGGTGACGGT GCAGGAGGGC 
CTGTGTGTCC ATGTGCCCTG CTCCTTCTCC TACCCCCAGG ATGGCTGGAC TGACTCTGAC 
CCAGTTCATG GCTACTGGTT CCGGGCAGGA GACAGACCAT ACCAAGACGC TCCAGTGGCC 
ACAAACAACC CAGACAGAGA AGTGCAGGCA GAGACCCAGG GCCGATTCCA ACTCCTTGGG 
GACATTTGGA GCAACGACTG CTCCCTGAGC ATCAGAGACG CCAGGAAGAG GGATAAGGGG 
TCATATTTCT TTCGGCTAGA GAGAGGAAGC ATGAAATGGA GTTACAAATC ACAGTTGAAT 
TACAAAACTA AGCAGCTGTC TGTGTTTGTG ACAGCCCTGA CCCATAGGCC TGACATCCTC 
ATCCTAGGGA CCCTAGAGTC TGGCCACTCC AGGAACCTGA CCTGCTCTGT GCCCTGGGCC 
TGTAAGCAGG GGACACCCCC CATGATCTCC TGGATTGGGG CCTCCGTGTC CTCCCCGGGC 
CCCACTACTG CCCGCTCCTC AGTGCTCACC CTTACCCCAA AGCCCCAGGA CCACGGCACC 
AGCCTCACCT GTCAGGTGAC CTTGCCTGGG ACAGGTGTGA CCACGACCAG TACCGTCCGC 
CTCGATGTGT CCTACCCTCC TTGGAACTTG ACCATGACTG TCTTCCAAGG AGATGCCACA 
GCATCCACAG CCCTGGGAAA TGGCTCATCT CTTTCAGTCC TTGAGGGCCA GTCTCTGCGC 
CTGGTCTGTG CTGTCAACAG CAATCCCCCT GCCAGGCTGA GCTGGACCCG GGGGAGCCTG 
ACCCTGTGCC CCTCACGGTC CTCAAACCCT GGGCTGCTGG AGCTGCCTCG AGTGCACGTG 
AGGGATGAAG GGGAATTCAC CTGCCGAGCT CAGAACGCTC AGGGCTCCCA GCACATTTCC 
CTGAGCCTCT CCCTGCAGAA TGAGGGCACA GGCACCTCAA GACCTGTATC ACAAGTGACA 
CTGGCAGCAG TCGGGGGAGC TGGAGCCACA GCCCTGGCCT TCCTGTCCTT CTGCATCATC 
TTCATCATAG TGAGGTCCTG CAGGAAGAAA TCGGCAAGGC CAGCAGCGGG CGTGGGGGAT 
ACAGGCATGG AAGATGCAAA GGCCATCAGG GGCTCGGCCT CTCAGGGACC CCTGACTGAA 
TCCTGGAAAG ATGGCAACCC CCTGAAGAAG CCTCCCCCAG CTGTTGCCCC CTCGTCAGGG 
GAGGAAGGAG AGCTCCATTA TGCAACCCTC AGCTTCCATA AAGTGAAGCC TCAGGACCCG 
CAGGGACAGG AGGCCACTGA CAGTGAATAC TCGGAGATCA AGATCCACAA GCGAGAAACT 
GCAGAGACTC AGGCCTGTTT GAGGAATCAC AACCCCTCCA GCAAAGAAGT CAGAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIGLEC8 ( NM_014442.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055257.2 Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8), mRNA.

Price & Availability↑

CloneID	OHu06385
Clone ID Related Accession (Same CDS sequence)	NM_014442.3 , NM_014442.2
Accession Version	NM_014442.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	sialic acid-binding Ig-like lectin 8 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA388103.1, BC053319.1 and AF195092.1. On May 4, 2006 this sequence version replaced NM_014442.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053319.1, SRR1803611.271576.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159764 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGC TGCTGCTGCT GCTGCCCCTG CTCTGGGGGA CAAAGGGGAT GGAGGGAGAC 
AGACAATATG GGGATGGTTA CTTGCTGCAA GTGCAGGAGC TGGTGACGGT GCAGGAGGGC 
CTGTGTGTCC ATGTGCCCTG CTCCTTCTCC TACCCCCAGG ATGGCTGGAC TGACTCTGAC 
CCAGTTCATG GCTACTGGTT CCGGGCAGGA GACAGACCAT ACCAAGACGC TCCAGTGGCC 
ACAAACAACC CAGACAGAGA AGTGCAGGCA GAGACCCAGG GCCGATTCCA ACTCCTTGGG 
GACATTTGGA GCAACGACTG CTCCCTGAGC ATCAGAGACG CCAGGAAGAG GGATAAGGGG 
TCATATTTCT TTCGGCTAGA GAGAGGAAGC ATGAAATGGA GTTACAAATC ACAGTTGAAT 
TACAAAACTA AGCAGCTGTC TGTGTTTGTG ACAGCCCTGA CCCATAGGCC TGACATCCTC 
ATCCTAGGGA CCCTAGAGTC TGGCCACTCC AGGAACCTGA CCTGCTCTGT GCCCTGGGCC 
TGTAAGCAGG GGACACCCCC CATGATCTCC TGGATTGGGG CCTCCGTGTC CTCCCCGGGC 
CCCACTACTG CCCGCTCCTC AGTGCTCACC CTTACCCCAA AGCCCCAGGA CCACGGCACC 
AGCCTCACCT GTCAGGTGAC CTTGCCTGGG ACAGGTGTGA CCACGACCAG TACCGTCCGC 
CTCGATGTGT CCTACCCTCC TTGGAACTTG ACCATGACTG TCTTCCAAGG AGATGCCACA 
GCATCCACAG CCCTGGGAAA TGGCTCATCT CTTTCAGTCC TTGAGGGCCA GTCTCTGCGC 
CTGGTCTGTG CTGTCAACAG CAATCCCCCT GCCAGGCTGA GCTGGACCCG GGGGAGCCTG 
ACCCTGTGCC CCTCACGGTC CTCAAACCCT GGGCTGCTGG AGCTGCCTCG AGTGCACGTG 
AGGGATGAAG GGGAATTCAC CTGCCGAGCT CAGAACGCTC AGGGCTCCCA GCACATTTCC 
CTGAGCCTCT CCCTGCAGAA TGAGGGCACA GGCACCTCAA GACCTGTATC ACAAGTGACA 
CTGGCAGCAG TCGGGGGAGC TGGAGCCACA GCCCTGGCCT TCCTGTCCTT CTGCATCATC 
TTCATCATAG TGAGGTCCTG CAGGAAGAAA TCGGCAAGGC CAGCAGCGGG CGTGGGGGAT 
ACAGGCATGG AAGATGCAAA GGCCATCAGG GGCTCGGCCT CTCAGGGACC CCTGACTGAA 
TCCTGGAAAG ATGGCAACCC CCTGAAGAAG CCTCCCCCAG CTGTTGCCCC CTCGTCAGGG 
GAGGAAGGAG AGCTCCATTA TGCAACCCTC AGCTTCCATA AAGTGAAGCC TCAGGACCCG 
CAGGGACAGG AGGCCACTGA CAGTGAATAC TCGGAGATCA AGATCCACAA GCGAGAAACT 
GCAGAGACTC AGGCCTGTTT GAGGAATCAC AACCCCTCCA GCAAAGAAGT CAGAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055257.2
CDS	68..1567
Translation	MLLLLLLLPLLWGTKGMEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTALTHRPDILILGTLESGHSRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLTLTPKPQDHGTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAAVGGAGATALAFLSFCIIFIIVRSCRKKSARPAAGVGDTGMEDAKAIRGSASQGPLTESWKDGNPLKKPPPAVAPSSGEEGELHYATLSFHKVKPQDPQGQEATDSEYSEIKIHKRETAETQACLRNHNPSSKEVRG

Target ORF information:

RefSeq Version	NM_014442.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014442.2

ORF Insert Sequence:

ATGCTGCTGC TGCTGCTGCT GCTGCCCCTG CTCTGGGGGA CAAAGGGGAT GGAGGGAGAC 
AGACAATATG GGGATGGTTA CTTGCTGCAA GTGCAGGAGC TGGTGACGGT GCAGGAGGGC 
CTGTGTGTCC ATGTGCCCTG CTCCTTCTCC TACCCCCAGG ATGGCTGGAC TGACTCTGAC 
CCAGTTCATG GCTACTGGTT CCGGGCAGGA GACAGACCAT ACCAAGACGC TCCAGTGGCC 
ACAAACAACC CAGACAGAGA AGTGCAGGCA GAGACCCAGG GCCGATTCCA ACTCCTTGGG 
GACATTTGGA GCAACGACTG CTCCCTGAGC ATCAGAGACG CCAGGAAGAG GGATAAGGGG 
TCATATTTCT TTCGGCTAGA GAGAGGAAGC ATGAAATGGA GTTACAAATC ACAGTTGAAT 
TACAAAACTA AGCAGCTGTC TGTGTTTGTG ACAGCCCTGA CCCATAGGCC TGACATCCTC 
ATCCTAGGGA CCCTAGAGTC TGGCCACTCC AGGAACCTGA CCTGCTCTGT GCCCTGGGCC 
TGTAAGCAGG GGACACCCCC CATGATCTCC TGGATTGGGG CCTCCGTGTC CTCCCCGGGC 
CCCACTACTG CCCGCTCCTC AGTGCTCACC CTTACCCCAA AGCCCCAGGA CCACGGCACC 
AGCCTCACCT GTCAGGTGAC CTTGCCTGGG ACAGGTGTGA CCACGACCAG TACCGTCCGC 
CTCGATGTGT CCTACCCTCC TTGGAACTTG ACCATGACTG TCTTCCAAGG AGATGCCACA 
GCATCCACAG CCCTGGGAAA TGGCTCATCT CTTTCAGTCC TTGAGGGCCA GTCTCTGCGC 
CTGGTCTGTG CTGTCAACAG CAATCCCCCT GCCAGGCTGA GCTGGACCCG GGGGAGCCTG 
ACCCTGTGCC CCTCACGGTC CTCAAACCCT GGGCTGCTGG AGCTGCCTCG AGTGCACGTG 
AGGGATGAAG GGGAATTCAC CTGCCGAGCT CAGAACGCTC AGGGCTCCCA GCACATTTCC 
CTGAGCCTCT CCCTGCAGAA TGAGGGCACA GGCACCTCAA GACCTGTATC ACAAGTGACA 
CTGGCAGCAG TCGGGGGAGC TGGAGCCACA GCCCTGGCCT TCCTGTCCTT CTGCATCATC 
TTCATCATAG TGAGGTCCTG CAGGAAGAAA TCGGCAAGGC CAGCAGCGGG CGTGGGGGAT 
ACAGGCATGG AAGATGCAAA GGCCATCAGG GGCTCGGCCT CTCAGGGACC CCTGACTGAA 
TCCTGGAAAG ATGGCAACCC CCTGAAGAAG CCTCCCCCAG CTGTTGCCCC CTCGTCAGGG 
GAGGAAGGAG AGCTCCATTA TGCAACCCTC AGCTTCCATA AAGTGAAGCC TCAGGACCCG 
CAGGGACAGG AGGCCACTGA CAGTGAATAC TCGGAGATCA AGATCCACAA GCGAGAAACT 
GCAGAGACTC AGGCCTGTTT GAGGAATCAC AACCCCTCCA GCAAAGAAGT CAGAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIGLEC8 ( NM_001363548.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350477.1 Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu67134
Clone ID Related Accession (Same CDS sequence)	NM_001363548.1 , XM_011526735.2
Accession Version	NM_001363548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	sialic acid-binding Ig-like lectin 8 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA388103.1, BC053319.1, BP309940.1 and AF195092.1. On May 24, 2018 this sequence version replaced XM_011526735.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.592987.1, SRR1803613.128924.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGC TGCTGCTGCT GCTGCCCCTG CTCTGGGGGA CAAAGGGGAT GGAGGGAGAC 
AGACAATATG GGGATGGTTA CTTGCTGCAA GTGCAGGAGC TGGTGACGGT GCAGGAGGGC 
CTGTGTGTCC ATGTGCCCTG CTCCTTCTCC TACCCCCAGG ATGGCTGGAC TGACTCTGAC 
CCAGTTCATG GCTACTGGTT CCGGGCAGGA GACAGACCAT ACCAAGACGC TCCAGTGGCC 
ACAAACAACC CAGACAGAGA AGTGCAGGCA GAGACCCAGG GCCGATTCCA ACTCCTTGGG 
GACATTTGGA GCAACGACTG CTCCCTGAGC ATCAGAGACG CCAGGAAGAG GGATAAGGGG 
TCATATTTCT TTCGGCTAGA GAGAGGAAGC ATGAAATGGA GTTACAAATC ACAGTTGAAT 
TACAAAACTA AGCAGCTGTC TGTGTTTGTG ACAGACCCTC CTTGGAACTT GACCATGACT 
GTCTTCCAAG GAGATGCCAC AGCATCCACA GCCCTGGGAA ATGGCTCATC TCTTTCAGTC 
CTTGAGGGCC AGTCTCTGCG CCTGGTCTGT GCTGTCAACA GCAATCCCCC TGCCAGGCTG 
AGCTGGACCC GGGGGAGCCT GACCCTGTGC CCCTCACGGT CCTCAAACCC TGGGCTGCTG 
GAGCTGCCTC GAGTGCACGT GAGGGATGAA GGGGAATTCA CCTGCCGAGC TCAGAACGCT 
CAGGGCTCCC AGCACATTTC CCTGAGCCTC TCCCTGCAGA ATGAGGGCAC AGGCACCTCA 
AGACCTGTAT CACAAGTGAC ACTGGCAGCA GTCGGGGGAG CTGGAGCCAC AGCCCTGGCC 
TTCCTGTCCT TCTGCATCAT CTTCATCATA GTGAGGTCCT GCAGGAAGAA ATCGGCAAGG 
CCAGCAGCGG GCGTGGGGGA TACAGGCATG GAAGATGCAA AGGCCATCAG GGGCTCGGCC 
TCTCAGGGAC CCCTGACTGA ATCCTGGAAA GATGGCAACC CCCTGAAGAA GCCTCCCCCA 
GCTGTTGCCC CCTCGTCAGG GGAGGAAGGA GAGCTCCATT ATGCAACCCT CAGCTTCCAT 
AAAGTGAAGC CTCAGGACCC GCAGGGACAG GAGGCCACTG ACAGTGAATA CTCGGAGATC 
AAGATCCACA AGCGAGAAAC TGCAGAGACT CAGGCCTGTT TGAGGAATCA CAACCCCTCC 
AGCAAAGAAG TCAGAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350477.1
CDS	68..1288
Translation	MLLLLLLLPLLWGTKGMEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTDPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAAVGGAGATALAFLSFCIIFIIVRSCRKKSARPAAGVGDTGMEDAKAIRGSASQGPLTESWKDGNPLKKPPPAVAPSSGEEGELHYATLSFHKVKPQDPQGQEATDSEYSEIKIHKRETAETQACLRNHNPSSKEVRG

Target ORF information:

RefSeq Version	NM_001363548.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363548.1

ORF Insert Sequence:

ATGCTGCTGC TGCTGCTGCT GCTGCCCCTG CTCTGGGGGA CAAAGGGGAT GGAGGGAGAC 
AGACAATATG GGGATGGTTA CTTGCTGCAA GTGCAGGAGC TGGTGACGGT GCAGGAGGGC 
CTGTGTGTCC ATGTGCCCTG CTCCTTCTCC TACCCCCAGG ATGGCTGGAC TGACTCTGAC 
CCAGTTCATG GCTACTGGTT CCGGGCAGGA GACAGACCAT ACCAAGACGC TCCAGTGGCC 
ACAAACAACC CAGACAGAGA AGTGCAGGCA GAGACCCAGG GCCGATTCCA ACTCCTTGGG 
GACATTTGGA GCAACGACTG CTCCCTGAGC ATCAGAGACG CCAGGAAGAG GGATAAGGGG 
TCATATTTCT TTCGGCTAGA GAGAGGAAGC ATGAAATGGA GTTACAAATC ACAGTTGAAT 
TACAAAACTA AGCAGCTGTC TGTGTTTGTG ACAGACCCTC CTTGGAACTT GACCATGACT 
GTCTTCCAAG GAGATGCCAC AGCATCCACA GCCCTGGGAA ATGGCTCATC TCTTTCAGTC 
CTTGAGGGCC AGTCTCTGCG CCTGGTCTGT GCTGTCAACA GCAATCCCCC TGCCAGGCTG 
AGCTGGACCC GGGGGAGCCT GACCCTGTGC CCCTCACGGT CCTCAAACCC TGGGCTGCTG 
GAGCTGCCTC GAGTGCACGT GAGGGATGAA GGGGAATTCA CCTGCCGAGC TCAGAACGCT 
CAGGGCTCCC AGCACATTTC CCTGAGCCTC TCCCTGCAGA ATGAGGGCAC AGGCACCTCA 
AGACCTGTAT CACAAGTGAC ACTGGCAGCA GTCGGGGGAG CTGGAGCCAC AGCCCTGGCC 
TTCCTGTCCT TCTGCATCAT CTTCATCATA GTGAGGTCCT GCAGGAAGAA ATCGGCAAGG 
CCAGCAGCGG GCGTGGGGGA TACAGGCATG GAAGATGCAA AGGCCATCAG GGGCTCGGCC 
TCTCAGGGAC CCCTGACTGA ATCCTGGAAA GATGGCAACC CCCTGAAGAA GCCTCCCCCA 
GCTGTTGCCC CCTCGTCAGG GGAGGAAGGA GAGCTCCATT ATGCAACCCT CAGCTTCCAT 
AAAGTGAAGC CTCAGGACCC GCAGGGACAG GAGGCCACTG ACAGTGAATA CTCGGAGATC 
AAGATCCACA AGCGAGAAAC TGCAGAGACT CAGGCCTGTT TGAGGAATCA CAACCCCTCC 
AGCAAAGAAG TCAGAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIGLEC8 ( XM_011526734.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525036.1 Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu67133
Clone ID Related Accession (Same CDS sequence)	XM_011526734.2
Accession Version	XM_011526734.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sialic acid-binding Ig-like lectin 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011526734.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC TGCTGCTGCT GCTGCCCCTG CTCTGGGGGA CAAAGGGGAT GGAGGGAGAC 
AGACAATATG GGGATGGTTA CTTGCTGCAA GTGCAGGAGC TGGTGACGGT GCAGGAGGGC 
CTGTGTGTCC ATGTGCCCTG CTCCTTCTCC TACCCCCAGG ATGGCTGGAC TGACTCTGAC 
CCAGTTCATG GCTACTGGTT CCGGGCAGGA GACAGACCAT ACCAAGACGC TCCAGTGGCC 
ACAAACAACC CAGACAGAGA AGTGCAGGCA GAGACCCAGG GCCGATTCCA ACTCCTTGGG 
GACATTTGGA GCAACGACTG CTCCCTGAGC ATCAGAGACG CCAGGAAGAG GGATAAGGGG 
TCATATTTCT TTCGGCTAGA GAGAGGAAGC ATGAAATGGA GTTACAAATC ACAGTTGAAT 
TACAAAACTA AGCAGCTGTC TGTGTTTGTG ACAGGGACCC TAGAGTCTGG CCACTCCAGG 
AACCTGACCT GCTCTGTGCC CTGGGCCTGT AAGCAGGGGA CACCCCCCAT GATCTCCTGG 
ATTGGGGCCT CCGTGTCCTC CCCGGGCCCC ACTACTGCCC GCTCCTCAGT GCTCACCCTT 
ACCCCAAAGC CCCAGGACCA CGGCACCAGC CTCACCTGTC AGGTGACCTT GCCTGGGACA 
GGTGTGACCA CGACCAGTAC CGTCCGCCTC GATGTGTCCT ACCCTCCTTG GAACTTGACC 
ATGACTGTCT TCCAAGGAGA TGCCACAGCA TCCACAGCCC TGGGAAATGG CTCATCTCTT 
TCAGTCCTTG AGGGCCAGTC TCTGCGCCTG GTCTGTGCTG TCAACAGCAA TCCCCCTGCC 
AGGCTGAGCT GGACCCGGGG GAGCCTGACC CTGTGCCCCT CACGGTCCTC AAACCCTGGG 
CTGCTGGAGC TGCCTCGAGT GCACGTGAGG GATGAAGGGG AATTCACCTG CCGAGCTCAG 
AACGCTCAGG GCTCCCAGCA CATTTCCCTG AGCCTCTCCC TGCAGAATGA GGGCACAGGC 
ACCTCAAGAC CTGTATCACA AGTGACACTG GCAGCAGTCG GGGGAGCTGG AGCCACAGCC 
CTGGCCTTCC TGTCCTTCTG CATCATCTTC ATCATAGTGA GGTCCTGCAG GAAGAAATCG 
GCAAGGCCAG CAGCGGGCGT GGGGGATACA GGCATGGAAG ATGCAAAGGC CATCAGGGGC 
TCGGCCTCTC AGGGACCCCT GACTGAATCC TGGAAAGATG GCAACCCCCT GAAGAAGCCT 
CCCCCAGCTG TTGCCCCCTC GTCAGGGGAG GAAGGAGAGC TCCATTATGC AACCCTCAGC 
TTCCATAAAG TGAAGCCTCA GGACCCGCAG GGACAGGAGG CCACTGACAG TGAATACTCG 
GAGATCAAGA TCCACAAGCG AGAAACTGCA GAGACTCAGG CCTGTTTGAG GAATCACAAC 
CCCTCCAGCA AAGAAGTCAG AGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525036.1
CDS	62..1528
Translation	MLLLLLLLPLLWGTKGMEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTGTLESGHSRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLTLTPKPQDHGTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAAVGGAGATALAFLSFCIIFIIVRSCRKKSARPAAGVGDTGMEDAKAIRGSASQGPLTESWKDGNPLKKPPPAVAPSSGEEGELHYATLSFHKVKPQDPQGQEATDSEYSEIKIHKRETAETQACLRNHNPSSKEVRG

Target ORF information:

RefSeq Version	XM_011526734.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011526734.2

ORF Insert Sequence:

ATGCTGCTGC TGCTGCTGCT GCTGCCCCTG CTCTGGGGGA CAAAGGGGAT GGAGGGAGAC 
AGACAATATG GGGATGGTTA CTTGCTGCAA GTGCAGGAGC TGGTGACGGT GCAGGAGGGC 
CTGTGTGTCC ATGTGCCCTG CTCCTTCTCC TACCCCCAGG ATGGCTGGAC TGACTCTGAC 
CCAGTTCATG GCTACTGGTT CCGGGCAGGA GACAGACCAT ACCAAGACGC TCCAGTGGCC 
ACAAACAACC CAGACAGAGA AGTGCAGGCA GAGACCCAGG GCCGATTCCA ACTCCTTGGG 
GACATTTGGA GCAACGACTG CTCCCTGAGC ATCAGAGACG CCAGGAAGAG GGATAAGGGG 
TCATATTTCT TTCGGCTAGA GAGAGGAAGC ATGAAATGGA GTTACAAATC ACAGTTGAAT 
TACAAAACTA AGCAGCTGTC TGTGTTTGTG ACAGGGACCC TAGAGTCTGG CCACTCCAGG 
AACCTGACCT GCTCTGTGCC CTGGGCCTGT AAGCAGGGGA CACCCCCCAT GATCTCCTGG 
ATTGGGGCCT CCGTGTCCTC CCCGGGCCCC ACTACTGCCC GCTCCTCAGT GCTCACCCTT 
ACCCCAAAGC CCCAGGACCA CGGCACCAGC CTCACCTGTC AGGTGACCTT GCCTGGGACA 
GGTGTGACCA CGACCAGTAC CGTCCGCCTC GATGTGTCCT ACCCTCCTTG GAACTTGACC 
ATGACTGTCT TCCAAGGAGA TGCCACAGCA TCCACAGCCC TGGGAAATGG CTCATCTCTT 
TCAGTCCTTG AGGGCCAGTC TCTGCGCCTG GTCTGTGCTG TCAACAGCAA TCCCCCTGCC 
AGGCTGAGCT GGACCCGGGG GAGCCTGACC CTGTGCCCCT CACGGTCCTC AAACCCTGGG 
CTGCTGGAGC TGCCTCGAGT GCACGTGAGG GATGAAGGGG AATTCACCTG CCGAGCTCAG 
AACGCTCAGG GCTCCCAGCA CATTTCCCTG AGCCTCTCCC TGCAGAATGA GGGCACAGGC 
ACCTCAAGAC CTGTATCACA AGTGACACTG GCAGCAGTCG GGGGAGCTGG AGCCACAGCC 
CTGGCCTTCC TGTCCTTCTG CATCATCTTC ATCATAGTGA GGTCCTGCAG GAAGAAATCG 
GCAAGGCCAG CAGCGGGCGT GGGGGATACA GGCATGGAAG ATGCAAAGGC CATCAGGGGC 
TCGGCCTCTC AGGGACCCCT GACTGAATCC TGGAAAGATG GCAACCCCCT GAAGAAGCCT 
CCCCCAGCTG TTGCCCCCTC GTCAGGGGAG GAAGGAGAGC TCCATTATGC AACCCTCAGC 
TTCCATAAAG TGAAGCCTCA GGACCCGCAG GGACAGGAGG CCACTGACAG TGAATACTCG 
GAGATCAAGA TCCACAAGCG AGAAACTGCA GAGACTCAGG CCTGTTTGAG GAATCACAAC 
CCCTCCAGCA AAGAAGTCAG AGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIGLEC8 ( XM_011526735.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525037.1 Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu67134
Clone ID Related Accession (Same CDS sequence)	NM_001363548.1 , XM_011526735.2
Accession Version	XM_011526735.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sialic acid-binding Ig-like lectin 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011526735.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC TGCTGCTGCT GCTGCCCCTG CTCTGGGGGA CAAAGGGGAT GGAGGGAGAC 
AGACAATATG GGGATGGTTA CTTGCTGCAA GTGCAGGAGC TGGTGACGGT GCAGGAGGGC 
CTGTGTGTCC ATGTGCCCTG CTCCTTCTCC TACCCCCAGG ATGGCTGGAC TGACTCTGAC 
CCAGTTCATG GCTACTGGTT CCGGGCAGGA GACAGACCAT ACCAAGACGC TCCAGTGGCC 
ACAAACAACC CAGACAGAGA AGTGCAGGCA GAGACCCAGG GCCGATTCCA ACTCCTTGGG 
GACATTTGGA GCAACGACTG CTCCCTGAGC ATCAGAGACG CCAGGAAGAG GGATAAGGGG 
TCATATTTCT TTCGGCTAGA GAGAGGAAGC ATGAAATGGA GTTACAAATC ACAGTTGAAT 
TACAAAACTA AGCAGCTGTC TGTGTTTGTG ACAGACCCTC CTTGGAACTT GACCATGACT 
GTCTTCCAAG GAGATGCCAC AGCATCCACA GCCCTGGGAA ATGGCTCATC TCTTTCAGTC 
CTTGAGGGCC AGTCTCTGCG CCTGGTCTGT GCTGTCAACA GCAATCCCCC TGCCAGGCTG 
AGCTGGACCC GGGGGAGCCT GACCCTGTGC CCCTCACGGT CCTCAAACCC TGGGCTGCTG 
GAGCTGCCTC GAGTGCACGT GAGGGATGAA GGGGAATTCA CCTGCCGAGC TCAGAACGCT 
CAGGGCTCCC AGCACATTTC CCTGAGCCTC TCCCTGCAGA ATGAGGGCAC AGGCACCTCA 
AGACCTGTAT CACAAGTGAC ACTGGCAGCA GTCGGGGGAG CTGGAGCCAC AGCCCTGGCC 
TTCCTGTCCT TCTGCATCAT CTTCATCATA GTGAGGTCCT GCAGGAAGAA ATCGGCAAGG 
CCAGCAGCGG GCGTGGGGGA TACAGGCATG GAAGATGCAA AGGCCATCAG GGGCTCGGCC 
TCTCAGGGAC CCCTGACTGA ATCCTGGAAA GATGGCAACC CCCTGAAGAA GCCTCCCCCA 
GCTGTTGCCC CCTCGTCAGG GGAGGAAGGA GAGCTCCATT ATGCAACCCT CAGCTTCCAT 
AAAGTGAAGC CTCAGGACCC GCAGGGACAG GAGGCCACTG ACAGTGAATA CTCGGAGATC 
AAGATCCACA AGCGAGAAAC TGCAGAGACT CAGGCCTGTT TGAGGAATCA CAACCCCTCC 
AGCAAAGAAG TCAGAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525037.1
CDS	64..1284
Translation	MLLLLLLLPLLWGTKGMEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTDPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAAVGGAGATALAFLSFCIIFIIVRSCRKKSARPAAGVGDTGMEDAKAIRGSASQGPLTESWKDGNPLKKPPPAVAPSSGEEGELHYATLSFHKVKPQDPQGQEATDSEYSEIKIHKRETAETQACLRNHNPSSKEVRG

Target ORF information:

RefSeq Version	XM_011526735.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011526735.2

ORF Insert Sequence:

ATGCTGCTGC TGCTGCTGCT GCTGCCCCTG CTCTGGGGGA CAAAGGGGAT GGAGGGAGAC 
AGACAATATG GGGATGGTTA CTTGCTGCAA GTGCAGGAGC TGGTGACGGT GCAGGAGGGC 
CTGTGTGTCC ATGTGCCCTG CTCCTTCTCC TACCCCCAGG ATGGCTGGAC TGACTCTGAC 
CCAGTTCATG GCTACTGGTT CCGGGCAGGA GACAGACCAT ACCAAGACGC TCCAGTGGCC 
ACAAACAACC CAGACAGAGA AGTGCAGGCA GAGACCCAGG GCCGATTCCA ACTCCTTGGG 
GACATTTGGA GCAACGACTG CTCCCTGAGC ATCAGAGACG CCAGGAAGAG GGATAAGGGG 
TCATATTTCT TTCGGCTAGA GAGAGGAAGC ATGAAATGGA GTTACAAATC ACAGTTGAAT 
TACAAAACTA AGCAGCTGTC TGTGTTTGTG ACAGACCCTC CTTGGAACTT GACCATGACT 
GTCTTCCAAG GAGATGCCAC AGCATCCACA GCCCTGGGAA ATGGCTCATC TCTTTCAGTC 
CTTGAGGGCC AGTCTCTGCG CCTGGTCTGT GCTGTCAACA GCAATCCCCC TGCCAGGCTG 
AGCTGGACCC GGGGGAGCCT GACCCTGTGC CCCTCACGGT CCTCAAACCC TGGGCTGCTG 
GAGCTGCCTC GAGTGCACGT GAGGGATGAA GGGGAATTCA CCTGCCGAGC TCAGAACGCT 
CAGGGCTCCC AGCACATTTC CCTGAGCCTC TCCCTGCAGA ATGAGGGCAC AGGCACCTCA 
AGACCTGTAT CACAAGTGAC ACTGGCAGCA GTCGGGGGAG CTGGAGCCAC AGCCCTGGCC 
TTCCTGTCCT TCTGCATCAT CTTCATCATA GTGAGGTCCT GCAGGAAGAA ATCGGCAAGG 
CCAGCAGCGG GCGTGGGGGA TACAGGCATG GAAGATGCAA AGGCCATCAG GGGCTCGGCC 
TCTCAGGGAC CCCTGACTGA ATCCTGGAAA GATGGCAACC CCCTGAAGAA GCCTCCCCCA 
GCTGTTGCCC CCTCGTCAGG GGAGGAAGGA GAGCTCCATT ATGCAACCCT CAGCTTCCAT 
AAAGTGAAGC CTCAGGACCC GCAGGGACAG GAGGCCACTG ACAGTGAATA CTCGGAGATC 
AAGATCCACA AGCGAGAAAC TGCAGAGACT CAGGCCTGTT TGAGGAATCA CAACCCCTCC 
AGCAAAGAAG TCAGAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sialic acid-binding immunoglobulin-like lectin 8 (Siglec-8) is an activating receptor mediating ?
The Journal of allergy and clinical immunology(2017 Sep)
Carroll DJ,O'Sullivan JA,Nix DB,Cao Y,Tiemeyer M,Bochner BS

Leveraging Siglec-8 endocytic mechanisms to kill human eosinophils and malignant mast cells.
The Journal of allergy and clinical immunology(2017 Jul)
O'Sullivan JA,Carroll DJ,Cao Y,Salicru AN,Bochner BS

Olig2 is expressed late in human eosinophil development and controls Siglec-8 expression.
Journal of leukocyte biology100(4)711-723(2016 10)
Hwang SM,Uhm TG,Lee SK,Kong SK,Jung KH,Binas B,Chai YG,Park SW,Chung IY

Decreased expression of Siglec-8 associates with poor prognosis in patients with gastric cancer after surgical resection.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(8)10883-91(2016 Aug)
Cao Y,Liu H,Zhang H,Lin C,Li R,Zhang W,Shen Z,Xu J

Role of MiR-215 in Hirschsprung's Disease Pathogenesis by Targeting SIGLEC-8.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology40(6)1646-1655(2016)
Lei H,Li H,Xie H,Du C,Xia Y,Tang W

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SIGLEC8 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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