DKK2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DKK2
Entrez Gene ID	27123
Full Name	dickkopf WNT signaling pathway inhibitor 2
Synonyms	DKK-2
General protein information	Preferred Names dickkopf WNT signaling pathway inhibitor 2 Names dickkopf-related protein 2 dickkopf 2 homolog dickkopf homolog 2 dickkopf-2 hDkk-2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q25
Summary	This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6). [provided by RefSeq, Jul 2008].
Disorder MIM:	605415

mRNA and Protein(s)

mRNA	Protein	Name
NM_014421.3	NP_055236.1	dickkopf-related protein 2 precursor
NM_014421.2	NP_055236.1	dickkopf-related protein 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	DKK2	NP_055236.1
Pan troglodytes (chimpanzee)	DKK2	XP_001170814.1
Canis lupus familiaris (dog)	DKK2	XP_535681.3
Danio rerio (zebrafish)	dkk2	NP_001104679.1
Rattus norvegicus (Norway rat)	Dkk2	NP_001099942.1
	DKK2	XP_001085254.1
Bos taurus (cattle)	DKK2	NP_001076084.1
Mus musculus (house mouse)	Dkk2	NP_064661.2
Gallus gallus (chicken)	DKK2	XP_420494.1
Xenopus tropicalis (tropical clawed frog)	dkk2	XP_002940336.1

Gene Ontology
Bibliography

Related articles in PubMed

Dickkopf-related protein 2 induces G0/G1 arrest and apoptosis through suppressing Wnt/?-catenin signaling and is frequently methylated in breast cancer.
Mu J, Hui T, Shao B, Li L, Du Z, Lu L, Ye L, Li S, Li Q, Xiao Q, Qiu Z, Zhang Y, Fan J, Ren G, Tao Q, Xiang T
Oncotarget8(24)39443-39459(2017 Jun)

Concurrent Hypermethylation of
Wang H, Duan XL, Qi XL, Meng L, Xu YS, Wu T, Dai PG
Molecules and cells40(1)45-53(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

epigenetic silencing of Wnt antagonists was associated with gastric carcinogenesis, and concurrent hypermethylation of SFRP2 and DKK2 could be a potential marker for a prognosis of poor overall survival.
Title: Concurrent Hypermethylation of

targeting of DKK2 by miR-154 leads to upregulation of beta-catenin expression and activation of the classical Wnt signaling pathway and cardiac fibroblasts.
Title: MiR-154 directly suppresses DKK2 to activate Wnt signaling pathway and enhance activation of cardiac fibroblasts.

The present report suggested that DKK2 downregulation suppressed the proliferation and invasion of prostate cancer cells by inhibiting the Wnt/betacatenin signaling pathway.
Title: Dickkopf?2?promotes proliferation and invasion via Wnt signaling in prostate cancer.

Knock down of the dickkopf WNT signaling pathway inhibitor 2 (DKK2) resulted in a significant suppression of HOXD10, HOXD11 and HOXD13 while over-expression of DKK2 and stimulation with factors of the WNT signaling pathway.
Title: The posterior HOXD locus: Its contribution to phenotype and malignancy of Ewing sarcoma.

these findings reveal that the miR-187-5p-DKK2 pathway regulates Wnt/beta-catenin signaling, cell growth, and apoptosis. Our findings provide the first evidence of a role for miR-187-5p in promotion of B-cell ALL.
Title: miR-187-5p Regulates Cell Growth and Apoptosis in Acute Lymphoblastic Leukemia via DKK2.

The following DKK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DKK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31152	NM_014421.3 Latest version!	Homo sapiens dickkopf WNT signaling pathway inhibitor 2 (DKK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
View Old Accession Versions >>
OHu31152	NM_014421.2	Homo sapiens dickkopf WNT signaling pathway inhibitor 2 (DKK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DKK2 ( NM_014421.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055236.1 Homo sapiens dickkopf WNT signaling pathway inhibitor 2 (DKK2), mRNA.

Price & Availability↑

CloneID	OHu31152
Clone ID Related Accession (Same CDS sequence)	NM_014421.3 , NM_014421.2
Accession Version	NM_014421.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	dickkopf-related protein 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001819.2, AF177395.1 and AY358414.1. On Nov 22, 2018 this sequence version replaced NM_014421.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF177395.1, SRR1660807.85450.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000285311.8/ ENSP00000285311.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCGCGT TGATGCGGAG CAAGGATTCG TCCTGCTGCC TGCTCCTACT GGCCGCGGTG 
CTGATGGTGG AGAGCTCACA GATCGGCAGT TCGCGGGCCA AACTCAACTC CATCAAGTCC 
TCTCTGGGCG GGGAGACGCC TGGTCAGGCC GCCAATCGAT CTGCGGGCAT GTACCAAGGA 
CTGGCATTCG GCGGCAGTAA GAAGGGCAAA AACCTGGGGC AGGCCTACCC TTGTAGCAGT 
GATAAGGAGT GTGAAGTTGG GAGGTATTGC CACAGTCCCC ACCAAGGATC ATCGGCCTGC 
ATGGTGTGTC GGAGAAAAAA GAAGCGCTGC CACCGAGATG GCATGTGCTG CCCCAGTACC 
CGCTGCAATA ATGGCATCTG TATCCCAGTT ACTGAAAGCA TCTTAACCCC TCACATCCCG 
GCTCTGGATG GTACTCGGCA CAGAGATCGA AACCACGGTC ATTACTCAAA CCATGACTTG 
GGATGGCAGA ATCTAGGAAG ACCACACACT AAGATGTCAC ATATAAAAGG GCATGAAGGA 
GACCCCTGCC TACGATCATC AGACTGCATT GAAGGGTTTT GCTGTGCTCG TCATTTCTGG 
ACCAAAATCT GCAAACCAGT GCTCCATCAG GGGGAAGTCT GTACCAAACA ACGCAAGAAG 
GGTTCTCATG GGCTGGAAAT TTTCCAGCGT TGCGACTGTG CGAAGGGCCT GTCTTGCAAA 
GTATGGAAAG ATGCCACCTA CTCCTCCAAA GCCAGACTCC ATGTGTGTCA GAAAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055236.1
CDS	723..1502
Translation	MAALMRSKDSSCCLLLLAAVLMVESSQIGSSRAKLNSIKSSLGGETPGQAANRSAGMYQGLAFGGSKKGKNLGQAYPCSSDKECEVGRYCHSPHQGSSACMVCRRKKKRCHRDGMCCPSTRCNNGICIPVTESILTPHIPALDGTRHRDRNHGHYSNHDLGWQNLGRPHTKMSHIKGHEGDPCLRSSDCIEGFCCARHFWTKICKPVLHQGEVCTKQRKKGSHGLEIFQRCDCAKGLSCKVWKDATYSSKARLHVCQKI

Target ORF information:

RefSeq Version	NM_014421.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dickkopf WNT signaling pathway inhibitor 2 (DKK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014421.3

ORF Insert Sequence:

ATGGCCGCGT TGATGCGGAG CAAGGATTCG TCCTGCTGCC TGCTCCTACT GGCCGCGGTG 
CTGATGGTGG AGAGCTCACA GATCGGCAGT TCGCGGGCCA AACTCAACTC CATCAAGTCC 
TCTCTGGGCG GGGAGACGCC TGGTCAGGCC GCCAATCGAT CTGCGGGCAT GTACCAAGGA 
CTGGCATTCG GCGGCAGTAA GAAGGGCAAA AACCTGGGGC AGGCCTACCC TTGTAGCAGT 
GATAAGGAGT GTGAAGTTGG GAGGTATTGC CACAGTCCCC ACCAAGGATC ATCGGCCTGC 
ATGGTGTGTC GGAGAAAAAA GAAGCGCTGC CACCGAGATG GCATGTGCTG CCCCAGTACC 
CGCTGCAATA ATGGCATCTG TATCCCAGTT ACTGAAAGCA TCTTAACCCC TCACATCCCG 
GCTCTGGATG GTACTCGGCA CAGAGATCGA AACCACGGTC ATTACTCAAA CCATGACTTG 
GGATGGCAGA ATCTAGGAAG ACCACACACT AAGATGTCAC ATATAAAAGG GCATGAAGGA 
GACCCCTGCC TACGATCATC AGACTGCATT GAAGGGTTTT GCTGTGCTCG TCATTTCTGG 
ACCAAAATCT GCAAACCAGT GCTCCATCAG GGGGAAGTCT GTACCAAACA ACGCAAGAAG 
GGTTCTCATG GGCTGGAAAT TTTCCAGCGT TGCGACTGTG CGAAGGGCCT GTCTTGCAAA 
GTATGGAAAG ATGCCACCTA CTCCTCCAAA GCCAGACTCC ATGTGTGTCA GAAAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DKK2 ( NM_014421.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055236.1 Homo sapiens dickkopf WNT signaling pathway inhibitor 2 (DKK2), mRNA.

Price & Availability↑

CloneID	OHu31152
Clone ID Related Accession (Same CDS sequence)	NM_014421.3 , NM_014421.2
Accession Version	NM_014421.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	dickkopf-related protein 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF177395.1 and AY358414.1. On Oct 28, 2004 this sequence version replaced NM_014421.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF177395.1, SRR1660807.85450.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCGT TGATGCGGAG CAAGGATTCG TCCTGCTGCC TGCTCCTACT GGCCGCGGTG 
CTGATGGTGG AGAGCTCACA GATCGGCAGT TCGCGGGCCA AACTCAACTC CATCAAGTCC 
TCTCTGGGCG GGGAGACGCC TGGTCAGGCC GCCAATCGAT CTGCGGGCAT GTACCAAGGA 
CTGGCATTCG GCGGCAGTAA GAAGGGCAAA AACCTGGGGC AGGCCTACCC TTGTAGCAGT 
GATAAGGAGT GTGAAGTTGG GAGGTATTGC CACAGTCCCC ACCAAGGATC ATCGGCCTGC 
ATGGTGTGTC GGAGAAAAAA GAAGCGCTGC CACCGAGATG GCATGTGCTG CCCCAGTACC 
CGCTGCAATA ATGGCATCTG TATCCCAGTT ACTGAAAGCA TCTTAACCCC TCACATCCCG 
GCTCTGGATG GTACTCGGCA CAGAGATCGA AACCACGGTC ATTACTCAAA CCATGACTTG 
GGATGGCAGA ATCTAGGAAG ACCACACACT AAGATGTCAC ATATAAAAGG GCATGAAGGA 
GACCCCTGCC TACGATCATC AGACTGCATT GAAGGGTTTT GCTGTGCTCG TCATTTCTGG 
ACCAAAATCT GCAAACCAGT GCTCCATCAG GGGGAAGTCT GTACCAAACA ACGCAAGAAG 
GGTTCTCATG GGCTGGAAAT TTTCCAGCGT TGCGACTGTG CGAAGGGCCT GTCTTGCAAA 
GTATGGAAAG ATGCCACCTA CTCCTCCAAA GCCAGACTCC ATGTGTGTCA GAAAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055236.1
CDS	706..1485
Translation	MAALMRSKDSSCCLLLLAAVLMVESSQIGSSRAKLNSIKSSLGGETPGQAANRSAGMYQGLAFGGSKKGKNLGQAYPCSSDKECEVGRYCHSPHQGSSACMVCRRKKKRCHRDGMCCPSTRCNNGICIPVTESILTPHIPALDGTRHRDRNHGHYSNHDLGWQNLGRPHTKMSHIKGHEGDPCLRSSDCIEGFCCARHFWTKICKPVLHQGEVCTKQRKKGSHGLEIFQRCDCAKGLSCKVWKDATYSSKARLHVCQKI

Target ORF information:

RefSeq Version	NM_014421.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dickkopf WNT signaling pathway inhibitor 2 (DKK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014421.2

ORF Insert Sequence:

ATGGCCGCGT TGATGCGGAG CAAGGATTCG TCCTGCTGCC TGCTCCTACT GGCCGCGGTG 
CTGATGGTGG AGAGCTCACA GATCGGCAGT TCGCGGGCCA AACTCAACTC CATCAAGTCC 
TCTCTGGGCG GGGAGACGCC TGGTCAGGCC GCCAATCGAT CTGCGGGCAT GTACCAAGGA 
CTGGCATTCG GCGGCAGTAA GAAGGGCAAA AACCTGGGGC AGGCCTACCC TTGTAGCAGT 
GATAAGGAGT GTGAAGTTGG GAGGTATTGC CACAGTCCCC ACCAAGGATC ATCGGCCTGC 
ATGGTGTGTC GGAGAAAAAA GAAGCGCTGC CACCGAGATG GCATGTGCTG CCCCAGTACC 
CGCTGCAATA ATGGCATCTG TATCCCAGTT ACTGAAAGCA TCTTAACCCC TCACATCCCG 
GCTCTGGATG GTACTCGGCA CAGAGATCGA AACCACGGTC ATTACTCAAA CCATGACTTG 
GGATGGCAGA ATCTAGGAAG ACCACACACT AAGATGTCAC ATATAAAAGG GCATGAAGGA 
GACCCCTGCC TACGATCATC AGACTGCATT GAAGGGTTTT GCTGTGCTCG TCATTTCTGG 
ACCAAAATCT GCAAACCAGT GCTCCATCAG GGGGAAGTCT GTACCAAACA ACGCAAGAAG 
GGTTCTCATG GGCTGGAAAT TTTCCAGCGT TGCGACTGTG CGAAGGGCCT GTCTTGCAAA 
GTATGGAAAG ATGCCACCTA CTCCTCCAAA GCCAGACTCC ATGTGTGTCA GAAAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dickkopf-related protein 2 induces G0/G1 arrest and apoptosis through suppressing Wnt/?-catenin signaling and is frequently methylated in breast cancer.
Oncotarget8(24)39443-39459(2017 Jun)
Mu J,Hui T,Shao B,Li L,Du Z,Lu L,Ye L,Li S,Li Q,Xiao Q,Qiu Z,Zhang Y,Fan J,Ren G,Tao Q,Xiang T

Concurrent Hypermethylation of
Molecules and cells40(1)45-53(2017 Jan)
Wang H,Duan XL,Qi XL,Meng L,Xu YS,Wu T,Dai PG

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DKK2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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